AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056390.t1 MTR_4g067340 98.507 201 3 0 1 201 71 271 3.05e-140 392
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056390.t1 AT3G59520 82.090 201 34 1 1 201 71 269 1.17e-114 327

Find 42 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTGGGCACTTACCTTAT+TGG 0.205224 4.1:-33965397 MS.gene056390:CDS
TACTGGGCACTTACCTTATT+GGG 0.220182 4.1:-33965396 MS.gene056390:CDS
TCTTCCAAGTTGGAGCTCTT+TGG 0.276633 4.1:-33965567 MS.gene056390:CDS
GGTGGTTGTATCCGGGGTTT+TGG 0.310982 4.1:-33965712 MS.gene056390:CDS
CTTGTGTTGGTGGTTGTATC+CGG 0.344944 4.1:-33965720 MS.gene056390:CDS
TTCATGGGATGACTAATTAC+TGG 0.352877 4.1:-33965413 MS.gene056390:CDS
TGAGTGCACTTTGGAGTCTT+GGG 0.365778 4.1:-33965800 MS.gene056390:CDS
TCAAACGGAGCAAAACTAAT+AGG 0.372282 4.1:+33965531 None:intergenic
ATAGCTTGGGGTTTGATTCA+TGG 0.377631 4.1:-33965429 MS.gene056390:CDS
TGTTGGATATGCTATAGCTT+GGG 0.379122 4.1:-33965442 MS.gene056390:CDS
AAGAACAATCCAACCCAATA+AGG 0.380469 4.1:+33965383 None:intergenic
CACTTCGATTATTGTTCCTC+AGG 0.392946 4.1:-33965496 MS.gene056390:CDS
TAGCTTGGGGTTTGATTCAT+GGG 0.402889 4.1:-33965428 MS.gene056390:CDS
CTGGCAATGCACTCATCTGC+AGG 0.403334 4.1:+33965241 None:intergenic
CATTTATCTGGGATTGTTGT+TGG 0.403713 4.1:-33965459 MS.gene056390:CDS
ATGAGTGCACTTTGGAGTCT+TGG 0.411154 4.1:-33965801 MS.gene056390:CDS
TCTTGGGGTTGTAGAGCAAT+TGG 0.420492 4.1:-33965784 MS.gene056390:CDS
TTGTGTTGGTGGTTGTATCC+GGG 0.423966 4.1:-33965719 MS.gene056390:CDS
CTTGTTGATTCAAAGATTCA+AGG 0.430998 4.1:-33965673 MS.gene056390:CDS
GGGCACTTACCTTATTGGGT+TGG 0.473467 4.1:-33965392 MS.gene056390:CDS
TTTCGTCGAGTGACGGCTGT+TGG 0.482713 4.1:-33965642 MS.gene056390:CDS
GCAGTATACACTTGTGTTGG+TGG 0.485085 4.1:-33965730 MS.gene056390:CDS
TTGTTGGATATGCTATAGCT+TGG 0.497279 4.1:-33965443 MS.gene056390:CDS
ACAATGTCAACATTTCCATC+TGG 0.497361 4.1:+33965222 None:intergenic
AAATCCAAAGAGCTCCAACT+TGG 0.500176 4.1:+33965563 None:intergenic
GAAGCAACCTTCTTCCAAGT+TGG 0.503075 4.1:-33965577 MS.gene056390:CDS
CAATTGGATCATGTTGGCCT+TGG 0.510981 4.1:-33965768 MS.gene056390:CDS
AATCGCACAGACGGTAATGA+AGG 0.514515 4.1:+33965288 None:intergenic
AAGAGCTCCAACTTGGAAGA+AGG 0.537007 4.1:+33965570 None:intergenic
TCATGGGATGACTAATTACT+GGG 0.568103 4.1:-33965412 MS.gene056390:CDS
GTGAAAATAAGCGATTCAAA+CGG 0.570383 4.1:+33965516 None:intergenic
GTTGGATATGCTATAGCTTG+GGG 0.574371 4.1:-33965441 MS.gene056390:CDS
GAGTGCACTTTGGAGTCTTG+GGG 0.582155 4.1:-33965799 MS.gene056390:CDS
CACAGACGGTAATGAAGGAT+CGG 0.600871 4.1:+33965293 None:intergenic
TGGCAATGCACTCATCTGCA+GGG 0.609281 4.1:+33965242 None:intergenic
TTTGCAGTATACACTTGTGT+TGG 0.612877 4.1:-33965733 MS.gene056390:CDS
CAGATGAGTGCATTGCCAGA+TGG 0.613817 4.1:-33965237 MS.gene056390:CDS
GTAGAGCAATTGGATCATGT+TGG 0.632439 4.1:-33965774 MS.gene056390:CDS
GCAAATAATACTCGACACCA+AGG 0.639015 4.1:+33965751 None:intergenic
TGTGTTGGTGGTTGTATCCG+GGG 0.653479 4.1:-33965718 MS.gene056390:CDS
CATTGCCAGAACCAAAACCC+CGG 0.701359 4.1:+33965701 None:intergenic
TGTCCAATAAAACTTGCCTG+AGG 0.704440 4.1:+33965480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTATTGGACATTTATC+TGG - chr4.1:33965548-33965567 MS.gene056390:CDS 25.0%
!!! GTTTTATTGGACATTTATCT+GGG - chr4.1:33965549-33965568 MS.gene056390:CDS 25.0%
GTGAAAATAAGCGATTCAAA+CGG + chr4.1:33965506-33965525 None:intergenic 30.0%
!! CTTGTTGATTCAAAGATTCA+AGG - chr4.1:33965346-33965365 MS.gene056390:CDS 30.0%
!!! GTTTTGAGTTTGAAGAAATC+TGG - chr4.1:33965665-33965684 MS.gene056390:CDS 30.0%
AAGAACAATCCAACCCAATA+AGG + chr4.1:33965639-33965658 None:intergenic 35.0%
ACAATGTCAACATTTCCATC+TGG + chr4.1:33965800-33965819 None:intergenic 35.0%
TCAAACGGAGCAAAACTAAT+AGG + chr4.1:33965491-33965510 None:intergenic 35.0%
TCATGGGATGACTAATTACT+GGG - chr4.1:33965607-33965626 MS.gene056390:CDS 35.0%
TGTTGGATATGCTATAGCTT+GGG - chr4.1:33965577-33965596 MS.gene056390:CDS 35.0%
TTCATGGGATGACTAATTAC+TGG - chr4.1:33965606-33965625 MS.gene056390:CDS 35.0%
TTGTTGGATATGCTATAGCT+TGG - chr4.1:33965576-33965595 MS.gene056390:CDS 35.0%
TTTGCAGTATACACTTGTGT+TGG - chr4.1:33965286-33965305 MS.gene056390:CDS 35.0%
! CATTTATCTGGGATTGTTGT+TGG - chr4.1:33965560-33965579 MS.gene056390:CDS 35.0%
!!! ATTCGTGTGTTGTTTTTGGT+TGG - chr4.1:33965402-33965421 MS.gene056390:CDS 35.0%
!!! GGTTATTCGTGTGTTGTTTT+TGG - chr4.1:33965398-33965417 MS.gene056390:CDS 35.0%
AAATCCAAAGAGCTCCAACT+TGG + chr4.1:33965459-33965478 None:intergenic 40.0%
ATAGCTTGGGGTTTGATTCA+TGG - chr4.1:33965590-33965609 MS.gene056390:CDS 40.0%
CACTTCGATTATTGTTCCTC+AGG - chr4.1:33965523-33965542 MS.gene056390:CDS 40.0%
GCAAATAATACTCGACACCA+AGG + chr4.1:33965271-33965290 None:intergenic 40.0%
GTAGAGCAATTGGATCATGT+TGG - chr4.1:33965245-33965264 MS.gene056390:CDS 40.0%
GTTGGATATGCTATAGCTTG+GGG - chr4.1:33965578-33965597 MS.gene056390:CDS 40.0%
TACTGGGCACTTACCTTATT+GGG - chr4.1:33965623-33965642 MS.gene056390:CDS 40.0%
TAGCTTGGGGTTTGATTCAT+GGG - chr4.1:33965591-33965610 MS.gene056390:CDS 40.0%
TGTCCAATAAAACTTGCCTG+AGG + chr4.1:33965542-33965561 None:intergenic 40.0%
TTACTGGGCACTTACCTTAT+TGG - chr4.1:33965622-33965641 MS.gene056390:CDS 40.0%
! ATTACCGTCTGTGCGATTTT+TGG - chr4.1:33965736-33965755 MS.gene056390:CDS 40.0%
! TGAGTATTTTCGTCGAGTGA+CGG - chr4.1:33965370-33965389 MS.gene056390:CDS 40.0%
! TTACCGTCTGTGCGATTTTT+GGG - chr4.1:33965737-33965756 MS.gene056390:CDS 40.0%
!! GTTCCTCAGGCAAGTTTTAT+TGG - chr4.1:33965536-33965555 MS.gene056390:CDS 40.0%
!!! CGATTTTTGGGATCTAGCAA+TGG - chr4.1:33965749-33965768 MS.gene056390:CDS 40.0%
AAGAGCTCCAACTTGGAAGA+AGG + chr4.1:33965452-33965471 None:intergenic 45.0%
AATCGCACAGACGGTAATGA+AGG + chr4.1:33965734-33965753 None:intergenic 45.0%
CACAGACGGTAATGAAGGAT+CGG + chr4.1:33965729-33965748 None:intergenic 45.0%
GAAGCAACCTTCTTCCAAGT+TGG - chr4.1:33965442-33965461 MS.gene056390:CDS 45.0%
GATCCCAAAAATCGCACAGA+CGG + chr4.1:33965743-33965762 None:intergenic 45.0%
GCAGTATACACTTGTGTTGG+TGG - chr4.1:33965289-33965308 MS.gene056390:CDS 45.0%
TCTTGGGGTTGTAGAGCAAT+TGG - chr4.1:33965235-33965254 MS.gene056390:CDS 45.0%
! CAATTGGATCATGTTGGCCT+TGG - chr4.1:33965251-33965270 MS.gene056390:CDS 45.0%
! TCTTCCAAGTTGGAGCTCTT+TGG - chr4.1:33965452-33965471 MS.gene056390:CDS 45.0%
!! ATGAGTGCACTTTGGAGTCT+TGG - chr4.1:33965218-33965237 MS.gene056390:CDS 45.0%
!! CTTGTGTTGGTGGTTGTATC+CGG - chr4.1:33965299-33965318 MS.gene056390:CDS 45.0%
!! TGAGTGCACTTTGGAGTCTT+GGG - chr4.1:33965219-33965238 MS.gene056390:CDS 45.0%
!! TTGTGTTGGTGGTTGTATCC+GGG - chr4.1:33965300-33965319 MS.gene056390:CDS 45.0%
CAGATGAGTGCATTGCCAGA+TGG - chr4.1:33965782-33965801 MS.gene056390:CDS 50.0%
CATTGCCAGAACCAAAACCC+CGG + chr4.1:33965321-33965340 None:intergenic 50.0%
GGGCACTTACCTTATTGGGT+TGG - chr4.1:33965627-33965646 MS.gene056390:CDS 50.0%
TGGCAATGCACTCATCTGCA+GGG + chr4.1:33965780-33965799 None:intergenic 50.0%
!! GAGTGCACTTTGGAGTCTTG+GGG - chr4.1:33965220-33965239 MS.gene056390:CDS 50.0%
!! TGTGTTGGTGGTTGTATCCG+GGG - chr4.1:33965301-33965320 MS.gene056390:CDS 50.0%
!!! TGTATCCGGGGTTTTGGTTC+TGG - chr4.1:33965313-33965332 MS.gene056390:CDS 50.0%
CTGGCAATGCACTCATCTGC+AGG + chr4.1:33965781-33965800 None:intergenic 55.0%
TTTCGTCGAGTGACGGCTGT+TGG - chr4.1:33965377-33965396 MS.gene056390:CDS 55.0%
! GGTGGTTGTATCCGGGGTTT+TGG - chr4.1:33965307-33965326 MS.gene056390:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 33965218 33965823 33965218 ID=MS.gene056390
chr4.1 mRNA 33965218 33965823 33965218 ID=MS.gene056390.t1;Parent=MS.gene056390
chr4.1 exon 33965218 33965823 33965218 ID=MS.gene056390.t1.exon1;Parent=MS.gene056390.t1
chr4.1 CDS 33965218 33965823 33965218 ID=cds.MS.gene056390.t1;Parent=MS.gene056390.t1
Gene Sequence

>MS.gene056390

ATGAGTGCACTTTGGAGTCTTGGGGTTGTAGAGCAATTGGATCATGTTGGCCTTGGTGTCGAGTATTATTTGCAGTATACACTTGTGTTGGTGGTTGTATCCGGGGTTTTGGTTCTGGCAATGTATCACTTGTTGATTCAAAGATTCAAGGTTGAGTATTTTCGTCGAGTGACGGCTGTTGGTTATTCGTGTGTTGTTTTTGGTTGGATGACGATTCTTTCTGTGAAGCAACCTTCTTCCAAGTTGGAGCTCTTTGGATTTCTTTCGCTTCCTATTAGTTTTGCTCCGTTTGAATCGCTTATTTTCACTTCGATTATTGTTCCTCAGGCAAGTTTTATTGGACATTTATCTGGGATTGTTGTTGGATATGCTATAGCTTGGGGTTTGATTCATGGGATGACTAATTACTGGGCACTTACCTTATTGGGTTGGATTGTTCTTGTCTTTGTTTTGAGTTTGAAGAAATCTGGTGCACTTGACATAAGCTTCATTGAGATTGAATCTGTTACCGATCCTTCATTACCGTCTGTGCGATTTTTGGGATCTAGCAATGGCAGAACCCTGCAGATGAGTGCATTGCCAGATGGAAATGTTGACATTGTCTAA

Protein sequence

>MS.gene056390.t1

MSALWSLGVVEQLDHVGLGVEYYLQYTLVLVVVSGVLVLAMYHLLIQRFKVEYFRRVTAVGYSCVVFGWMTILSVKQPSSKLELFGFLSLPISFAPFESLIFTSIIVPQASFIGHLSGIVVGYAIAWGLIHGMTNYWALTLLGWIVLVFVLSLKKSGALDISFIEIESVTDPSLPSVRFLGSSNGRTLQMSALPDGNVDIV