Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89108.t1 | XP_003597774.1 | 99.4 | 311 | 0 | 1 | 1 | 309 | 1 | 311 | 1.90E-123 | 452.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89108.t1 | Q9S7T5 | 41.3 | 303 | 123 | 7 | 1 | 281 | 1 | 270 | 1.4e-26 | 121.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89108.t1 | G7II51 | 99.4 | 311 | 0 | 1 | 1 | 309 | 1 | 311 | 1.4e-123 | 452.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene89108.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049264 | MS.gene89108 | 0.803281 | 3.76E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene89108 | 0.821885 | 3.18E-53 | -1.69E-46 |
| MS.gene051210 | MS.gene89108 | 0.822847 | 1.90E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene89108 | 0.80402 | 2.64E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene89108 | 0.808531 | 2.96E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene89108 | 0.804707 | 1.90E-49 | -1.69E-46 |
| MS.gene054826 | MS.gene89108 | 0.831342 | 1.76E-55 | -1.69E-46 |
| MS.gene055701 | MS.gene89108 | 0.835199 | 1.93E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene89108 | 0.807633 | 4.60E-50 | -1.69E-46 |
| MS.gene057194 | MS.gene89108 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene89108 | 0.807954 | 3.93E-50 | -1.69E-46 |
| MS.gene059440 | MS.gene89108 | 0.806818 | 6.84E-50 | -1.69E-46 |
| MS.gene059952 | MS.gene89108 | 0.825567 | 4.37E-54 | -1.69E-46 |
| MS.gene06052 | MS.gene89108 | 0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene060850 | MS.gene89108 | 0.805891 | 1.07E-49 | -1.69E-46 |
| MS.gene060851 | MS.gene89108 | 0.813247 | 2.82E-51 | -1.69E-46 |
| MS.gene060852 | MS.gene89108 | 0.812386 | 4.35E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene89108 | 0.809761 | 1.61E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene89108 | 0.804867 | 1.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89108.t1 | MTR_2g102140 | 99.035 | 311 | 1 | 1 | 1 | 309 | 1 | 311 | 0.0 | 630 |
| MS.gene89108.t1 | MTR_4g012060 | 46.562 | 320 | 130 | 8 | 1 | 309 | 1 | 290 | 1.33e-76 | 236 |
| MS.gene89108.t1 | MTR_1g072110 | 38.406 | 138 | 61 | 4 | 140 | 271 | 115 | 234 | 2.37e-18 | 82.8 |
| MS.gene89108.t1 | MTR_7g095150 | 47.887 | 71 | 35 | 1 | 201 | 271 | 132 | 200 | 4.61e-15 | 73.2 |
| MS.gene89108.t1 | MTR_1g111640 | 28.340 | 247 | 96 | 9 | 29 | 268 | 26 | 198 | 5.88e-13 | 67.8 |
| MS.gene89108.t1 | MTR_1g103640 | 42.188 | 64 | 34 | 1 | 206 | 269 | 117 | 177 | 5.03e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89108.t1 | AT1G79960 | 36.335 | 322 | 147 | 10 | 1 | 298 | 1 | 288 | 6.06e-47 | 160 |
| MS.gene89108.t1 | AT5G04820 | 35.338 | 133 | 62 | 4 | 137 | 269 | 102 | 210 | 2.77e-14 | 72.0 |
| MS.gene89108.t1 | AT4G14860 | 45.312 | 64 | 28 | 2 | 207 | 268 | 109 | 167 | 1.54e-11 | 62.8 |
Find 51 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGTCCTTACGACGATTTC+AGG | 0.179517 | 2.4:-2803425 | MS.gene89108:CDS |
| AAGCAGGTTACCATAGAATT+TGG | 0.233067 | 2.4:-2803150 | MS.gene89108:CDS |
| GAAAATTGATTGGGACTTCA+TGG | 0.309098 | 2.4:-2803346 | MS.gene89108:CDS |
| TTTGCAGCCAGAGAGCATTT+TGG | 0.332403 | 2.4:+2803965 | None:intergenic |
| CAGGAACCACCAATACGTTT+TGG | 0.387081 | 2.4:+2803714 | None:intergenic |
| ACAATCAAAGGAAAATTGAT+TGG | 0.389239 | 2.4:-2803356 | MS.gene89108:CDS |
| CTAAGAGGAACCAAATTCTA+TGG | 0.390296 | 2.4:+2803140 | None:intergenic |
| TTTGTTGTTATTGTTGTTAA+CGG | 0.409570 | 2.4:+2803884 | None:intergenic |
| GTTAATCGAATTTGAGGCTG+TGG | 0.438345 | 2.4:+2803987 | None:intergenic |
| TATTGGTGGTTCCTGGTTGT+TGG | 0.439269 | 2.4:-2803706 | MS.gene89108:CDS |
| TTAGGATTGGTAGGGAAGTC+AGG | 0.440786 | 2.4:-2803185 | MS.gene89108:CDS |
| CAAAACGTATTGGTGGTTCC+TGG | 0.454127 | 2.4:-2803713 | MS.gene89108:CDS |
| GAGTTGGTGGAATCAAAGTA+TGG | 0.465659 | 2.4:-2803390 | MS.gene89108:CDS |
| GAAGAATTCAGAGGCTGCTC+CGG | 0.469764 | 2.4:-2803232 | MS.gene89108:CDS |
| CGATTAACCAAAATGCTCTC+TGG | 0.470779 | 2.4:-2803972 | MS.gene89108:CDS |
| CGTGTAGCGTGAGAACTGTT+AGG | 0.472831 | 2.4:-2803203 | MS.gene89108:CDS |
| AACAGTGCAACACAATTCTC+AGG | 0.479484 | 2.4:+2803459 | None:intergenic |
| AGGTAACTCAGGATCATTGA+TGG | 0.483746 | 2.4:-2803616 | MS.gene89108:CDS |
| ACACCACCTCAAGATCTTTG+TGG | 0.490451 | 2.4:-2803663 | MS.gene89108:CDS |
| CAATCAAAGGAAAATTGATT+GGG | 0.491479 | 2.4:-2803355 | MS.gene89108:CDS |
| TGAAATTCTCAAAGAGAAAA+CGG | 0.493044 | 2.4:+2803829 | None:intergenic |
| ATCAATGATCCTGAGTTACC+TGG | 0.499280 | 2.4:+2803618 | None:intergenic |
| AACAGCATCACCTTCAAAAG+AGG | 0.511692 | 2.4:-2803523 | MS.gene89108:CDS |
| TGAGAACTGTTAGGATTGGT+AGG | 0.512241 | 2.4:-2803194 | MS.gene89108:CDS |
| GCGATCAATGCAAGAGTTGG+TGG | 0.513161 | 2.4:-2803403 | MS.gene89108:CDS |
| CTGAAATCGTCGTAAGGACT+CGG | 0.524949 | 2.4:+2803426 | None:intergenic |
| TTTGTTGAGGTAATCTTGAA+GGG | 0.525082 | 2.4:+2804025 | None:intergenic |
| CAGGCGATCAATGCAAGAGT+TGG | 0.529425 | 2.4:-2803406 | MS.gene89108:CDS |
| TTGATCTGATCACTGTTATG+AGG | 0.534780 | 2.4:-2803254 | MS.gene89108:CDS |
| AAATTCTATGGTAACCTGCT+TGG | 0.538786 | 2.4:+2803152 | None:intergenic |
| CTCACGCTACACGGCTTCGC+CGG | 0.538841 | 2.4:+2803213 | None:intergenic |
| GAGGAACCACCAAAACGTAT+TGG | 0.549662 | 2.4:-2803723 | MS.gene89108:CDS |
| ATTGTTGTTAACGGCGTTTG+AGG | 0.555849 | 2.4:+2803893 | None:intergenic |
| GCACGATTCTTCGTGAAACC+AGG | 0.558059 | 2.4:-2803636 | MS.gene89108:CDS |
| TATGTTTCTGCCGTTATCCA+AGG | 0.558329 | 2.4:+2803920 | None:intergenic |
| CTAACAGTTCTCACGCTACA+CGG | 0.576486 | 2.4:+2803204 | None:intergenic |
| TTAATCGAATTTGAGGCTGT+GGG | 0.576513 | 2.4:+2803988 | None:intergenic |
| TTCGTGAAACCAGGTAACTC+AGG | 0.588008 | 2.4:-2803627 | MS.gene89108:CDS |
| AGCGTGAGAACTGTTAGGAT+TGG | 0.588361 | 2.4:-2803198 | MS.gene89108:CDS |
| CAGGAAGAAGAAGACCAAGC+AGG | 0.606262 | 2.4:-2803166 | MS.gene89108:CDS |
| TCTCGGTGACTCCAACAACC+AGG | 0.608261 | 2.4:+2803695 | None:intergenic |
| GTAAGATTGAAAACAATCAA+AGG | 0.608557 | 2.4:-2803368 | MS.gene89108:CDS |
| GAACCACCAAAACGTATTGG+TGG | 0.614019 | 2.4:-2803720 | MS.gene89108:CDS |
| GCTGAGCCACAAAGATCTTG+AGG | 0.618030 | 2.4:+2803657 | None:intergenic |
| TGTGATGATTGTGATCATCA+TGG | 0.625055 | 2.4:+2803487 | None:intergenic |
| GATCGCCTGAAATCGTCGTA+AGG | 0.629682 | 2.4:+2803420 | None:intergenic |
| GAGAACTGTTAGGATTGGTA+GGG | 0.631326 | 2.4:-2803193 | MS.gene89108:CDS |
| GGTGGTGTTGTAATGAATCT+CGG | 0.636568 | 2.4:+2803678 | None:intergenic |
| AATTGATTGGGACTTCATGG+AGG | 0.677814 | 2.4:-2803343 | MS.gene89108:CDS |
| TGTTATGAGGAAGAATTCAG+AGG | 0.682074 | 2.4:-2803241 | MS.gene89108:CDS |
| GAGCCACAAAGATCTTGAGG+TGG | 0.753269 | 2.4:+2803660 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGTTGTTATTGTTGTTAA+CGG | + | chr2.4:2803306-2803325 | None:intergenic | 20.0% |
| !!! | TTTTGTTTTTGATTTTGTTG+AGG | + | chr2.4:2803178-2803197 | None:intergenic | 20.0% |
| ! | ACAATCAAAGGAAAATTGAT+TGG | - | chr2.4:2803831-2803850 | MS.gene89108:CDS | 25.0% |
| ! | CAACAAAAACAAAAGAATCT+CGG | - | chr2.4:2803427-2803446 | MS.gene89108:CDS | 25.0% |
| ! | CAATCAAAGGAAAATTGATT+GGG | - | chr2.4:2803832-2803851 | MS.gene89108:CDS | 25.0% |
| ! | GTAAGATTGAAAACAATCAA+AGG | - | chr2.4:2803819-2803838 | MS.gene89108:CDS | 25.0% |
| ! | TGAAATTCTCAAAGAGAAAA+CGG | + | chr2.4:2803361-2803380 | None:intergenic | 25.0% |
| !!! | ATTTTGGTTAATCGAATTTG+AGG | + | chr2.4:2803209-2803228 | None:intergenic | 25.0% |
| TTTGTTGAGGTAATCTTGAA+GGG | + | chr2.4:2803165-2803184 | None:intergenic | 30.0% | |
| ! | AAGGAAAAAGATGGTGTTTT+GGG | + | chr2.4:2803251-2803270 | None:intergenic | 30.0% |
| ! | ACTACAATAACCTCTTTTGA+AGG | + | chr2.4:2803677-2803696 | None:intergenic | 30.0% |
| ! | TTTTGTTGAGGTAATCTTGA+AGG | + | chr2.4:2803166-2803185 | None:intergenic | 30.0% |
| AAATTCTATGGTAACCTGCT+TGG | + | chr2.4:2804038-2804057 | None:intergenic | 35.0% | |
| AAGCAGGTTACCATAGAATT+TGG | - | chr2.4:2804037-2804056 | MS.gene89108:CDS | 35.0% | |
| CTAAGAGGAACCAAATTCTA+TGG | + | chr2.4:2804050-2804069 | None:intergenic | 35.0% | |
| GAAAATTGATTGGGACTTCA+TGG | - | chr2.4:2803841-2803860 | MS.gene89108:CDS | 35.0% | |
| TGTGATGATTGTGATCATCA+TGG | + | chr2.4:2803703-2803722 | None:intergenic | 35.0% | |
| TGTTATGAGGAAGAATTCAG+AGG | - | chr2.4:2803946-2803965 | MS.gene89108:CDS | 35.0% | |
| TTGATCTGATCACTGTTATG+AGG | - | chr2.4:2803933-2803952 | MS.gene89108:CDS | 35.0% | |
| ! | CAAAACACCATCTTTTTCCT+TGG | - | chr2.4:2803250-2803269 | MS.gene89108:CDS | 35.0% |
| ! | CAAGGAAAAAGATGGTGTTT+TGG | + | chr2.4:2803252-2803271 | None:intergenic | 35.0% |
| ! | CCATCTTTTTCCTTGGATAA+CGG | - | chr2.4:2803257-2803276 | MS.gene89108:CDS | 35.0% |
| ! | TTAATCGAATTTGAGGCTGT+GGG | + | chr2.4:2803202-2803221 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTTGAAGCTTCTT+AGG | + | chr2.4:2803146-2803165 | None:intergenic | 35.0% |
| AACAGCATCACCTTCAAAAG+AGG | - | chr2.4:2803664-2803683 | MS.gene89108:CDS | 40.0% | |
| AACAGTGCAACACAATTCTC+AGG | + | chr2.4:2803731-2803750 | None:intergenic | 40.0% | |
| AATTGATTGGGACTTCATGG+AGG | - | chr2.4:2803844-2803863 | MS.gene89108:CDS | 40.0% | |
| AGGTAACTCAGGATCATTGA+TGG | - | chr2.4:2803571-2803590 | MS.gene89108:CDS | 40.0% | |
| ATCAATGATCCTGAGTTACC+TGG | + | chr2.4:2803572-2803591 | None:intergenic | 40.0% | |
| CCGTTATCCAAGGAAAAAGA+TGG | + | chr2.4:2803260-2803279 | None:intergenic | 40.0% | |
| CGATTAACCAAAATGCTCTC+TGG | - | chr2.4:2803215-2803234 | MS.gene89108:CDS | 40.0% | |
| CTCGGATGAAAAAAACAACG+AGG | - | chr2.4:2803445-2803464 | MS.gene89108:CDS | 40.0% | |
| ! | ATTGTTGTTAACGGCGTTTG+AGG | + | chr2.4:2803297-2803316 | None:intergenic | 40.0% |
| ! | GAGAACTGTTAGGATTGGTA+GGG | - | chr2.4:2803994-2804013 | MS.gene89108:CDS | 40.0% |
| ! | GTTAATCGAATTTGAGGCTG+TGG | + | chr2.4:2803203-2803222 | None:intergenic | 40.0% |
| ! | TATGTTTCTGCCGTTATCCA+AGG | + | chr2.4:2803270-2803289 | None:intergenic | 40.0% |
| ! | TGAGAACTGTTAGGATTGGT+AGG | - | chr2.4:2803993-2804012 | MS.gene89108:CDS | 40.0% |
| !! | GAGTTGGTGGAATCAAAGTA+TGG | - | chr2.4:2803797-2803816 | MS.gene89108:CDS | 40.0% |
| !! | GGTGGTGTTGTAATGAATCT+CGG | + | chr2.4:2803512-2803531 | None:intergenic | 40.0% |
| ACACCACCTCAAGATCTTTG+TGG | - | chr2.4:2803524-2803543 | MS.gene89108:CDS | 45.0% | |
| AGCGTGAGAACTGTTAGGAT+TGG | - | chr2.4:2803989-2804008 | MS.gene89108:CDS | 45.0% | |
| CTAACAGTTCTCACGCTACA+CGG | + | chr2.4:2803986-2804005 | None:intergenic | 45.0% | |
| CTGAAATCGTCGTAAGGACT+CGG | + | chr2.4:2803764-2803783 | None:intergenic | 45.0% | |
| GAACCACCAAAACGTATTGG+TGG | - | chr2.4:2803467-2803486 | MS.gene89108:CDS | 45.0% | |
| GAGGAACCACCAAAACGTAT+TGG | - | chr2.4:2803464-2803483 | MS.gene89108:CDS | 45.0% | |
| TTCGTGAAACCAGGTAACTC+AGG | - | chr2.4:2803560-2803579 | MS.gene89108:CDS | 45.0% | |
| ! | CAAAACGTATTGGTGGTTCC+TGG | - | chr2.4:2803474-2803493 | MS.gene89108:CDS | 45.0% |
| ! | CAGGAACCACCAATACGTTT+TGG | + | chr2.4:2803476-2803495 | None:intergenic | 45.0% |
| ! | GAACCACCAATACGTTTTGG+TGG | + | chr2.4:2803473-2803492 | None:intergenic | 45.0% |
| ! | TATTGGTGGTTCCTGGTTGT+TGG | - | chr2.4:2803481-2803500 | MS.gene89108:CDS | 45.0% |
| ! | TTAGGATTGGTAGGGAAGTC+AGG | - | chr2.4:2804002-2804021 | MS.gene89108:CDS | 45.0% |
| !!! | TTTGCAGCCAGAGAGCATTT+TGG | + | chr2.4:2803225-2803244 | None:intergenic | 45.0% |
| CAGGAAGAAGAAGACCAAGC+AGG | - | chr2.4:2804021-2804040 | MS.gene89108:CDS | 50.0% | |
| CAGGCGATCAATGCAAGAGT+TGG | - | chr2.4:2803781-2803800 | MS.gene89108:CDS | 50.0% | |
| CGAGTCCTTACGACGATTTC+AGG | - | chr2.4:2803762-2803781 | MS.gene89108:CDS | 50.0% | |
| CGTGTAGCGTGAGAACTGTT+AGG | - | chr2.4:2803984-2804003 | MS.gene89108:CDS | 50.0% | |
| GAAGAATTCAGAGGCTGCTC+CGG | - | chr2.4:2803955-2803974 | MS.gene89108:CDS | 50.0% | |
| GAGCCACAAAGATCTTGAGG+TGG | + | chr2.4:2803530-2803549 | None:intergenic | 50.0% | |
| GATCGCCTGAAATCGTCGTA+AGG | + | chr2.4:2803770-2803789 | None:intergenic | 50.0% | |
| GCACGATTCTTCGTGAAACC+AGG | - | chr2.4:2803551-2803570 | MS.gene89108:CDS | 50.0% | |
| GCGATCAATGCAAGAGTTGG+TGG | - | chr2.4:2803784-2803803 | MS.gene89108:CDS | 50.0% | |
| GCTGAGCCACAAAGATCTTG+AGG | + | chr2.4:2803533-2803552 | None:intergenic | 50.0% | |
| TCTCGGTGACTCCAACAACC+AGG | + | chr2.4:2803495-2803514 | None:intergenic | 55.0% | |
| CTCACGCTACACGGCTTCGC+CGG | + | chr2.4:2803977-2803996 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 2803140 | 2804069 | 2803140 | ID=MS.gene89108 |
| chr2.4 | mRNA | 2803140 | 2804069 | 2803140 | ID=MS.gene89108.t1;Parent=MS.gene89108 |
| chr2.4 | exon | 2803140 | 2804069 | 2803140 | ID=MS.gene89108.t1.exon1;Parent=MS.gene89108.t1 |
| chr2.4 | CDS | 2803140 | 2804069 | 2803140 | ID=cds.MS.gene89108.t1;Parent=MS.gene89108.t1 |
| Gene Sequence |
| Protein sequence |
