Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91255.t1 | KEH38887.1 | 97.7 | 44 | 1 | 0 | 48 | 91 | 201 | 244 | 4.30E-15 | 90.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91255.t1 | Q9MAT6 | 52.9 | 51 | 24 | 0 | 41 | 91 | 158 | 208 | 4.8e-07 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91255.t1 | A0A072VBD4 | 97.7 | 44 | 1 | 0 | 48 | 91 | 215 | 258 | 3.1e-15 | 90.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene91255 | 0.822282 | 2.58E-53 | -1.69E-46 |
| MS.gene050026 | MS.gene91255 | 0.801076 | 1.07E-48 | -1.69E-46 |
| MS.gene050156 | MS.gene91255 | 0.804474 | 2.12E-49 | -1.69E-46 |
| MS.gene050355 | MS.gene91255 | 0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene91255 | 0.806058 | 9.89E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene91255 | 0.805028 | 1.63E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene91255 | 0.815532 | 8.81E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene91255 | 0.813066 | 3.09E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene91255 | 0.830986 | 2.16E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene91255 | 0.821451 | 4.01E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene91255 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene91255 | 0.80513 | 1.55E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene91255 | 0.813207 | 2.88E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene91255 | -0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene91255 | 0.824985 | 6.00E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene91255 | 0.822367 | 2.46E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene91255 | 0.800625 | 1.32E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene91255 | 0.816628 | 5.01E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene91255 | 0.815011 | 1.15E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene91255 | 0.818714 | 1.70E-52 | -1.69E-46 |
| MS.gene056252 | MS.gene91255 | 0.801954 | 7.06E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene91255 | 0.818177 | 2.25E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene91255 | 0.802955 | 4.39E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene91255 | 0.803941 | 2.74E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene91255 | 0.808567 | 2.91E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene91255 | 0.808055 | 3.74E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91255.t1 | MTR_2g084410 | 66.038 | 106 | 10 | 4 | 1 | 91 | 164 | 258 | 1.21e-32 | 114 |
| MS.gene91255.t1 | MTR_2g084375 | 65.094 | 106 | 11 | 4 | 1 | 91 | 150 | 244 | 5.50e-32 | 112 |
| MS.gene91255.t1 | MTR_3g009890 | 77.193 | 57 | 13 | 0 | 35 | 91 | 155 | 211 | 8.60e-24 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGGATGCGCTCTTGGTTT+TGG | 0.248738 | 2.3:+13751440 | MS.gene91255:CDS |
| AGTGTCTGTGCTGGACTAAT+TGG | 0.366436 | 2.3:-13752365 | None:intergenic |
| AAAAGAAGAGGATGCGCTCT+TGG | 0.439362 | 2.3:+13751434 | MS.gene91255:CDS |
| ACTTCTTCCAAGAAAATAAG+AGG | 0.454192 | 2.3:+13751515 | MS.gene91255:CDS |
| ATATATAGTCACGGTGGACC+TGG | 0.469482 | 2.3:+13752433 | MS.gene91255:CDS |
| TCAGTGCATGGAACAATTGA+CGG | 0.496206 | 2.3:+13752395 | MS.gene91255:CDS |
| GAAGAACCGAGTGTCTGTGC+TGG | 0.576107 | 2.3:-13752374 | None:intergenic |
| CAGTGCATGGAACAATTGAC+GGG | 0.580953 | 2.3:+13752396 | MS.gene91255:CDS |
| GAGTTGAAAGAGACTTCTGC+AGG | 0.582564 | 2.3:+13751398 | MS.gene91255:CDS |
| CTGGGTTCTGAACAGCTGAA+AGG | 0.586271 | 2.3:+13752452 | MS.gene91255:CDS |
| TATATAGTCACGGTGGACCT+GGG | 0.588733 | 2.3:+13752434 | MS.gene91255:CDS |
| ACTTCTTCCAAGAAAAGAAG+AGG | 0.593684 | 2.3:+13751422 | MS.gene91255:CDS |
| CTTTCAGCTGTTCAGAACCC+AGG | 0.626176 | 2.3:-13752451 | None:intergenic |
| ATTGACGGGAAATTTGATGA+CGG | 0.626327 | 2.3:+13752410 | MS.gene91255:CDS |
| ACACTCGGTTCTTCAGTGCA+TGG | 0.628634 | 2.3:+13752383 | MS.gene91255:CDS |
| ATTAGTCCAGCACAGACACT+CGG | 0.635032 | 2.3:+13752368 | MS.gene91255:CDS |
| TGATGACGGATATATAGTCA+CGG | 0.676944 | 2.3:+13752424 | MS.gene91255:CDS |
| TGACGGATATATAGTCACGG+TGG | 0.799576 | 2.3:+13752427 | MS.gene91255:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATCTGATTTTTTTTGAAAC+TGG | + | chr2.3:13751790-13751809 | MS.gene91255:intron | 20.0% |
| ! | AACAAAGTTCTTATCTATGT+TGG | + | chr2.3:13752282-13752301 | MS.gene91255:intron | 25.0% |
| ! | ATTATGACATCGTTTAATCT+GGG | + | chr2.3:13751943-13751962 | MS.gene91255:intron | 25.0% |
| ! | TCAAAAAATAAGAGAGAAGA+AGG | + | chr2.3:13751464-13751483 | MS.gene91255:CDS | 25.0% |
| ! | TTGTATGTTTACAAGTTTCT+AGG | - | chr2.3:13751551-13751570 | None:intergenic | 25.0% |
| !! | TCGTTTCTTTAATTTAGACT+TGG | + | chr2.3:13751846-13751865 | MS.gene91255:intron | 25.0% |
| !! | TGAAATATTTTTCTGTGATG+TGG | + | chr2.3:13751688-13751707 | MS.gene91255:intron | 25.0% |
| !!! | TGTAATACCTCTTATTTTCT+TGG | - | chr2.3:13751525-13751544 | None:intergenic | 25.0% |
| ACTTCTTCCAAGAAAATAAG+AGG | + | chr2.3:13751515-13751534 | MS.gene91255:CDS | 30.0% | |
| AGAAACGAATCATCTATGAA+TGG | - | chr2.3:13751834-13751853 | None:intergenic | 30.0% | |
| CATTATGACATCGTTTAATC+TGG | + | chr2.3:13751942-13751961 | MS.gene91255:intron | 30.0% | |
| GGACGTATAGTCAATATTAT+TGG | - | chr2.3:13751594-13751613 | None:intergenic | 30.0% | |
| !!! | GAGTTTTGATTGACTTCTTT+TGG | + | chr2.3:13751730-13751749 | MS.gene91255:intron | 30.0% |
| !!! | TTCTGCAGAAGTAGTTTTTT+CGG | - | chr2.3:13751495-13751514 | None:intergenic | 30.0% |
| AAACTCTCCTAGAGAACTAA+TGG | + | chr2.3:13752114-13752133 | MS.gene91255:intron | 35.0% | |
| ACTTCTTCCAAGAAAAGAAG+AGG | + | chr2.3:13751422-13751441 | MS.gene91255:CDS | 35.0% | |
| ATGTATACCCTAACTCATGA+TGG | - | chr2.3:13751615-13751634 | None:intergenic | 35.0% | |
| ATTGACGGGAAATTTGATGA+CGG | + | chr2.3:13752410-13752429 | MS.gene91255:CDS | 35.0% | |
| GTGATGTGGAAATAGAAGTA+AGG | + | chr2.3:13751702-13751721 | MS.gene91255:intron | 35.0% | |
| TATACGTCCATCATGAGTTA+GGG | + | chr2.3:13751605-13751624 | MS.gene91255:intron | 35.0% | |
| TCTATCATAAGTTGTGTCGA+TGG | - | chr2.3:13751921-13751940 | None:intergenic | 35.0% | |
| !! | ATCGAAAGCTTTATTGCACT+AGG | - | chr2.3:13751981-13752000 | None:intergenic | 35.0% |
| !! | TGATGACGGATATATAGTCA+CGG | + | chr2.3:13752424-13752443 | MS.gene91255:CDS | 35.0% |
| AACAGTCACCTATGTGCTTA+TGG | - | chr2.3:13752028-13752047 | None:intergenic | 40.0% | |
| AAGCACATAGGTGACTGTTT+GGG | + | chr2.3:13752029-13752048 | MS.gene91255:intron | 40.0% | |
| ATGTACTGCCATAAGCACAT+AGG | + | chr2.3:13752017-13752036 | MS.gene91255:intron | 40.0% | |
| CTATACGTCCATCATGAGTT+AGG | + | chr2.3:13751604-13751623 | MS.gene91255:intron | 40.0% | |
| TAAGCACATAGGTGACTGTT+TGG | + | chr2.3:13752028-13752047 | MS.gene91255:intron | 40.0% | |
| TCAGTGCATGGAACAATTGA+CGG | + | chr2.3:13752395-13752414 | MS.gene91255:CDS | 40.0% | |
| TTGGGAGAGCTTATGGATAT+TGG | + | chr2.3:13752047-13752066 | MS.gene91255:intron | 40.0% | |
| ! | GCTGTTTCCATTAGTTCTCT+AGG | - | chr2.3:13752124-13752143 | None:intergenic | 40.0% |
| !!! | GCAGAAGTAGTTTTTTCGGA+AGG | - | chr2.3:13751491-13751510 | None:intergenic | 40.0% |
| AAAAGAAGAGGATGCGCTCT+TGG | + | chr2.3:13751434-13751453 | MS.gene91255:CDS | 45.0% | |
| AGTGTCTGTGCTGGACTAAT+TGG | - | chr2.3:13752368-13752387 | None:intergenic | 45.0% | |
| ATATATAGTCACGGTGGACC+TGG | + | chr2.3:13752433-13752452 | MS.gene91255:CDS | 45.0% | |
| ATTAGTCCAGCACAGACACT+CGG | + | chr2.3:13752368-13752387 | MS.gene91255:CDS | 45.0% | |
| CAGTGCATGGAACAATTGAC+GGG | + | chr2.3:13752396-13752415 | MS.gene91255:CDS | 45.0% | |
| GAGTTGAAAGAGACTTCTGC+AGG | + | chr2.3:13751398-13751417 | MS.gene91255:CDS | 45.0% | |
| TATATAGTCACGGTGGACCT+GGG | + | chr2.3:13752434-13752453 | MS.gene91255:CDS | 45.0% | |
| TGACTGTTTGGGAGAGCTTA+TGG | + | chr2.3:13752040-13752059 | MS.gene91255:intron | 45.0% | |
| ! | AGCGCATCCTCTTCTTTTCT+TGG | - | chr2.3:13751432-13751451 | None:intergenic | 45.0% |
| ! | TGACGGATATATAGTCACGG+TGG | + | chr2.3:13752427-13752446 | MS.gene91255:CDS | 45.0% |
| ACACTCGGTTCTTCAGTGCA+TGG | + | chr2.3:13752383-13752402 | MS.gene91255:CDS | 50.0% | |
| CTTTCAGCTGTTCAGAACCC+AGG | - | chr2.3:13752454-13752473 | None:intergenic | 50.0% | |
| ! | AGAGGATGCGCTCTTGGTTT+TGG | + | chr2.3:13751440-13751459 | MS.gene91255:CDS | 50.0% |
| !! | CTGGGTTCTGAACAGCTGAA+AGG | + | chr2.3:13752452-13752471 | MS.gene91255:CDS | 50.0% |
| GAAGAACCGAGTGTCTGTGC+TGG | - | chr2.3:13752377-13752396 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 13751395 | 13752493 | 13751395 | ID=MS.gene91255 |
| chr2.3 | mRNA | 13751395 | 13752493 | 13751395 | ID=MS.gene91255.t1;Parent=MS.gene91255 |
| chr2.3 | exon | 13751395 | 13751536 | 13751395 | ID=MS.gene91255.t1.exon1;Parent=MS.gene91255.t1 |
| chr2.3 | CDS | 13751395 | 13751536 | 13751395 | ID=cds.MS.gene91255.t1;Parent=MS.gene91255.t1 |
| chr2.3 | exon | 13752360 | 13752493 | 13752360 | ID=MS.gene91255.t1.exon2;Parent=MS.gene91255.t1 |
| chr2.3 | CDS | 13752360 | 13752493 | 13752360 | ID=cds.MS.gene91255.t1;Parent=MS.gene91255.t1 |
| Gene Sequence |
| Protein sequence |
