AlfalfaGEDB | MS.gene92758

M. sativa cultivar XinJiangDaYe / MS.gene92758

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92758.t1	XP_013449250.1	94	168	10	0	1	168	1	168	2.10E-76	295

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92758.t1	A0A072U0R7	94.0	168	10	0	1	168	1	168	1.5e-76	295.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049989	MS.gene92758	0.849882	2.43E-60	-1.69E-46
MS.gene050297	MS.gene92758	0.849627	2.86E-60	-1.69E-46
MS.gene050310	MS.gene92758	0.801768	7.70E-49	-1.69E-46
MS.gene051423	MS.gene92758	0.810901	9.16E-51	-1.69E-46
MS.gene051629	MS.gene92758	0.807948	3.94E-50	-1.69E-46
MS.gene051742	MS.gene92758	0.816857	4.46E-52	-1.69E-46
MS.gene052143	MS.gene92758	0.860738	1.68E-63	-1.69E-46
MS.gene052308	MS.gene92758	0.844779	6.12E-59	-1.69E-46
MS.gene052367	MS.gene92758	0.874496	6.45E-68	-1.69E-46
MS.gene053173	MS.gene92758	0.805322	1.41E-49	-1.69E-46
MS.gene053348	MS.gene92758	0.805821	1.11E-49	-1.69E-46
MS.gene053416	MS.gene92758	0.826532	2.58E-54	-1.69E-46
MS.gene053417	MS.gene92758	0.827778	1.30E-54	-1.69E-46
MS.gene054551	MS.gene92758	0.807631	4.60E-50	-1.69E-46
MS.gene055308	MS.gene92758	0.807967	3.90E-50	-1.69E-46
MS.gene055578	MS.gene92758	0.823601	1.27E-53	-1.69E-46
MS.gene055957	MS.gene92758	0.834409	3.05E-56	-1.69E-46
MS.gene056041	MS.gene92758	0.868094	8.43E-66	-1.69E-46
MS.gene056042	MS.gene92758	0.829189	5.92E-55	-1.69E-46
MS.gene056526	MS.gene92758	0.800326	1.52E-48	-1.69E-46
MS.gene056870	MS.gene92758	0.806948	6.42E-50	-1.69E-46
MS.gene057483	MS.gene92758	0.823801	1.14E-53	-1.69E-46
MS.gene058196	MS.gene92758	0.826422	2.74E-54	-1.69E-46
MS.gene058197	MS.gene92758	0.825856	3.73E-54	-1.69E-46
MS.gene058198	MS.gene92758	0.829549	4.84E-55	-1.69E-46
MS.gene058827	MS.gene92758	0.80337	3.60E-49	-1.69E-46
MS.gene058849	MS.gene92758	0.801504	8.73E-49	-1.69E-46
MS.gene058853	MS.gene92758	0.800719	1.26E-48	-1.69E-46
MS.gene059300	MS.gene92758	0.817826	2.70E-52	-1.69E-46
MS.gene060473	MS.gene92758	0.841158	5.63E-58	-1.69E-46
MS.gene060565	MS.gene92758	0.843506	1.34E-58	-1.69E-46
MS.gene06057	MS.gene92758	0.847312	1.25E-59	-1.69E-46
MS.gene060825	MS.gene92758	0.835844	1.32E-56	-1.69E-46
MS.gene061099	MS.gene92758	0.823088	1.67E-53	-1.69E-46
MS.gene061260	MS.gene92758	0.847491	1.12E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92758.t1	MTR_7g073500	94.048	168	10	0	1	168	1	168	8.15e-111	314

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene92758.t1	AT5G01910	46.715	137	61	2	19	155	4	128	1.74e-29	106
MS.gene92758.t1	AT5G01910	46.715	137	61	2	19	155	4	128	3.13e-29	106

Find 38 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATCGGCGGGAGTGTTTA+TGG	0.245932	7.4:-31102974	None:intergenic
CGATAGATTGTTCCGTTTGA+AGG	0.364571	7.4:+31103278	MS.gene92758:CDS
TAATTGATCGAGCTTGGGTT+TGG	0.365621	7.4:-31103019	None:intergenic
AAAACATAATTGATCGAGCT+TGG	0.395643	7.4:-31103025	None:intergenic
AATTGATCGAGCTTGGGTTT+GGG	0.402211	7.4:-31103018	None:intergenic
CCGAAAATCCGGCCACGCTC+TGG	0.408098	7.4:+31102934	MS.gene92758:CDS
GAGCTTGGGTTTGGGCTTTG+TGG	0.415984	7.4:-31103010	None:intergenic
CCACCGGAACACCGAAAATC+CGG	0.421575	7.4:+31102923	MS.gene92758:CDS
GTGTCCGGCCAGAGCGTGGC+CGG	0.445137	7.4:-31102942	None:intergenic
AAACATAATTGATCGAGCTT+GGG	0.445986	7.4:-31103024	None:intergenic
GTTTGGGCTTTGTGGAAAGA+TGG	0.456431	7.4:-31103002	None:intergenic
AGATCGGCGGGAGTGTTTAT+GGG	0.461473	7.4:-31102973	None:intergenic
CGCCACCGTGACTTCTCCAC+TGG	0.478503	7.4:+31103086	MS.gene92758:CDS
TATTCGAACCCTTAGAGAAA+AGG	0.487625	7.4:+31103416	MS.gene92758:CDS
GTTGCTTGACGCGAAGATGA+AGG	0.492065	7.4:+31103209	MS.gene92758:CDS
GAAGTCACGGTGGCGGTGAT+CGG	0.499844	7.4:-31103078	None:intergenic
AGATGGTGAGTGAGATCGGC+GGG	0.512562	7.4:-31102985	None:intergenic
GATATTTGAGTTCCTTCAAA+CGG	0.520675	7.4:-31103290	None:intergenic
AAATCCGGCCACGCTCTGGC+CGG	0.529930	7.4:+31102938	MS.gene92758:CDS
TCCACTGGAGACGTCGCTTG+CGG	0.547190	7.4:+31103101	MS.gene92758:CDS
TTATGGGCTGAAGTGGTGTC+CGG	0.550025	7.4:-31102957	None:intergenic
TCCGCAAGCGACGTCTCCAG+TGG	0.551194	7.4:-31103102	None:intergenic
GACCGCGATGAAGAAGAAGC+TGG	0.558819	7.4:+31103131	MS.gene92758:CDS
TGACGCGAAGATGAAGGAGA+TGG	0.559497	7.4:+31103215	MS.gene92758:CDS
AGTGGTGTCCGGCCAGAGCG+TGG	0.560975	7.4:-31102946	None:intergenic
ACTGGAGACGTCGCTTGCGG+AGG	0.568299	7.4:+31103104	MS.gene92758:CDS
GGAGTGTTTATGGGCTGAAG+TGG	0.581810	7.4:-31102964	None:intergenic
GGAAAGATGGTGAGTGAGAT+CGG	0.586737	7.4:-31102989	None:intergenic
CAGTGGAGAAGTCACGGTGG+CGG	0.593899	7.4:-31103085	None:intergenic
TGAAATCACACCGATCCAGA+AGG	0.600226	7.4:+31103047	MS.gene92758:CDS
CTTATGCAAACACCCGCCAC+CGG	0.606622	7.4:+31102907	MS.gene92758:CDS
TTTCATACCTCTGATGTCGA+TGG	0.613539	7.4:-31103468	None:intergenic
CGTCTCCAGTGGAGAAGTCA+CGG	0.624834	7.4:-31103091	None:intergenic
GGAACTCAAATATCGATGCA+TGG	0.636808	7.4:+31103299	MS.gene92758:CDS
CTCCAGCTTCTTCTTCATCG+CGG	0.643638	7.4:-31103133	None:intergenic
AACAGCACCATCGACATCAG+AGG	0.666142	7.4:+31103461	MS.gene92758:CDS
AAGATGGTGAGTGAGATCGG+CGG	0.726270	7.4:-31102986	None:intergenic
CTCCAGTGGAGAAGTCACGG+TGG	0.790221	7.4:-31103088	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAACATAATTGATCGAGCT+TGG	-	chr7.4:31103028-31103047	None:intergenic	30.0%
	AAACATAATTGATCGAGCTT+GGG	-	chr7.4:31103027-31103046	None:intergenic	30.0%
	AGAAACAAAACGAAACTGAA+TGG	-	chr7.4:31103353-31103372	None:intergenic	30.0%
!	GATATTTGAGTTCCTTCAAA+CGG	-	chr7.4:31103293-31103312	None:intergenic	30.0%
!	TTCATGTTCCTTTTCTCTAA+GGG	-	chr7.4:31103427-31103446	None:intergenic	30.0%
!	TTTCATGTTCCTTTTCTCTA+AGG	-	chr7.4:31103428-31103447	None:intergenic	30.0%
	TATTCGAACCCTTAGAGAAA+AGG	+	chr7.4:31103416-31103435	MS.gene92758:CDS	35.0%
!!	TTTTCTCTAAGGGTTCGAAT+AGG	-	chr7.4:31103417-31103436	None:intergenic	35.0%
	AATTGATCGAGCTTGGGTTT+GGG	-	chr7.4:31103021-31103040	None:intergenic	40.0%
	CGATAGATTGTTCCGTTTGA+AGG	+	chr7.4:31103278-31103297	MS.gene92758:CDS	40.0%
	GGAACTCAAATATCGATGCA+TGG	+	chr7.4:31103299-31103318	MS.gene92758:CDS	40.0%
	TAATTGATCGAGCTTGGGTT+TGG	-	chr7.4:31103022-31103041	None:intergenic	40.0%
	GGAAAGATGGTGAGTGAGAT+CGG	-	chr7.4:31102992-31103011	None:intergenic	45.0%
	TGAAATCACACCGATCCAGA+AGG	+	chr7.4:31103047-31103066	MS.gene92758:CDS	45.0%
!	GGACAGTTTTCCTTCTGGAT+CGG	-	chr7.4:31103060-31103079	None:intergenic	45.0%
!	GTTTGGGCTTTGTGGAAAGA+TGG	-	chr7.4:31103005-31103024	None:intergenic	45.0%
!	TGATCGGACAGTTTTCCTTC+TGG	-	chr7.4:31103065-31103084	None:intergenic	45.0%
	AACAGCACCATCGACATCAG+AGG	+	chr7.4:31103461-31103480	MS.gene92758:CDS	50.0%
	AAGATGGTGAGTGAGATCGG+CGG	-	chr7.4:31102989-31103008	None:intergenic	50.0%
	CTCCAGCTTCTTCTTCATCG+CGG	-	chr7.4:31103136-31103155	None:intergenic	50.0%
	GTTGCTTGACGCGAAGATGA+AGG	+	chr7.4:31103209-31103228	MS.gene92758:CDS	50.0%
	TGACGCGAAGATGAAGGAGA+TGG	+	chr7.4:31103215-31103234	MS.gene92758:CDS	50.0%
!	GGAGTGTTTATGGGCTGAAG+TGG	-	chr7.4:31102967-31102986	None:intergenic	50.0%
!	TTATGGGCTGAAGTGGTGTC+CGG	-	chr7.4:31102960-31102979	None:intergenic	50.0%
!!	AGATCGGCGGGAGTGTTTAT+GGG	-	chr7.4:31102976-31102995	None:intergenic	50.0%
	AGATGGTGAGTGAGATCGGC+GGG	-	chr7.4:31102988-31103007	None:intergenic	55.0%
	CCACCGGAACACCGAAAATC+CGG	+	chr7.4:31102923-31102942	MS.gene92758:CDS	55.0%
	CGTCTCCAGTGGAGAAGTCA+CGG	-	chr7.4:31103094-31103113	None:intergenic	55.0%
	CTTATGCAAACACCCGCCAC+CGG	+	chr7.4:31102907-31102926	MS.gene92758:CDS	55.0%
	GACCGCGATGAAGAAGAAGC+TGG	+	chr7.4:31103131-31103150	MS.gene92758:CDS	55.0%
	GAGCTTGGGTTTGGGCTTTG+TGG	-	chr7.4:31103013-31103032	None:intergenic	55.0%
!!	GAGATCGGCGGGAGTGTTTA+TGG	-	chr7.4:31102977-31102996	None:intergenic	55.0%
!!!	ATTTTCGGTGTTCCGGTGGC+GGG	-	chr7.4:31102922-31102941	None:intergenic	55.0%
!!!	GATTTTCGGTGTTCCGGTGG+CGG	-	chr7.4:31102923-31102942	None:intergenic	55.0%
!!!	TGGCCGGATTTTCGGTGTTC+CGG	-	chr7.4:31102929-31102948	None:intergenic	55.0%
	CAGTGGAGAAGTCACGGTGG+CGG	-	chr7.4:31103088-31103107	None:intergenic	60.0%
	CTCCAGTGGAGAAGTCACGG+TGG	-	chr7.4:31103091-31103110	None:intergenic	60.0%
	TCCACTGGAGACGTCGCTTG+CGG	+	chr7.4:31103101-31103120	MS.gene92758:CDS	60.0%
!	CCAGAGCGTGGCCGGATTTT+CGG	-	chr7.4:31102937-31102956	None:intergenic	60.0%
!	GAAGTCACGGTGGCGGTGAT+CGG	-	chr7.4:31103081-31103100	None:intergenic	60.0%
!!!	CCGGATTTTCGGTGTTCCGG+TGG	-	chr7.4:31102926-31102945	None:intergenic	60.0%
	AAATCCGGCCACGCTCTGGC+CGG	+	chr7.4:31102938-31102957	MS.gene92758:CDS	65.0%
	ACTGGAGACGTCGCTTGCGG+AGG	+	chr7.4:31103104-31103123	MS.gene92758:CDS	65.0%
	CCGAAAATCCGGCCACGCTC+TGG	+	chr7.4:31102934-31102953	MS.gene92758:CDS	65.0%
	CGCCACCGTGACTTCTCCAC+TGG	+	chr7.4:31103086-31103105	MS.gene92758:CDS	65.0%
	TCCGCAAGCGACGTCTCCAG+TGG	-	chr7.4:31103105-31103124	None:intergenic	65.0%
	AGTGGTGTCCGGCCAGAGCG+TGG	-	chr7.4:31102949-31102968	None:intergenic	70.0%
	GTGTCCGGCCAGAGCGTGGC+CGG	-	chr7.4:31102945-31102964	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	31102898	31103488	31102898	ID=MS.gene92758
chr7.4	mRNA	31102898	31103488	31102898	ID=MS.gene92758.t1;Parent=MS.gene92758
chr7.4	exon	31102898	31103320	31102898	ID=MS.gene92758.t1.exon1;Parent=MS.gene92758.t1
chr7.4	CDS	31102898	31103320	31102898	ID=cds.MS.gene92758.t1;Parent=MS.gene92758.t1
chr7.4	exon	31103405	31103488	31103405	ID=MS.gene92758.t1.exon2;Parent=MS.gene92758.t1
chr7.4	CDS	31103405	31103488	31103405	ID=cds.MS.gene92758.t1;Parent=MS.gene92758.t1

Gene Sequence

>MS.gene92758

ATGGCACAGCTTATGCAAACACCCGCCACCGGAACACCGAAAATCCGGCCACGCTCTGGCCGGACACCACTTCAGCCCATAAACACTCCCGCCGATCTCACTCACCATCTTTCCACAAAGCCCAAACCCAAGCTCGATCAATTATGTTTTGAAATCACACCGATCCAGAAGGAAAACTGTCCGATCACCGCCACCGTGACTTCTCCACTGGAGACGTCGCTTGCGGAGGAGCTGACCGCGATGAAGAAGAAGCTGGAGAGACTGAGAATAGACAAAGAAAAGACAGAGAAAATGCTGAAAGAGAGAGAAGCGTTGCTTGACGCGAAGATGAAGGAGATGGAAGAGAAAAGCGAGATTCAGAAGAATCTCGAAATTCAAGTCGATAGATTGTTCCGTTTGAAGGAACTCAAATATCGATGCATGGTGCGAACAATTAAGATTTAAAGTTTTCACCATTCAGTTTCGTTTTGTTTCTAATTTTGTTTTTTACTTTTTCGACTGTAACAGAGAGTTTCTCCTATTCGAACCCTTAGAGAAAAGGAACATGAAAAGATTATCAACGAAACAGCACCATCGACATCAGAGGTATGA

Protein sequence

>MS.gene92758.t1

MAQLMQTPATGTPKIRPRSGRTPLQPINTPADLTHHLSTKPKPKLDQLCFEITPIQKENCPITATVTSPLETSLAEELTAMKKKLERLRIDKEKTEKMLKEREALLDAKMKEMEEKSEIQKNLEIQVDRLFRLKELKYRCMRVSPIRTLREKEHEKIINETAPSTSEV