Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94401.t1 | XP_003615184.1 | 93.1 | 391 | 22 | 1 | 1 | 391 | 12 | 397 | 2.90E-169 | 604.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94401.t1 | Q9QYW0 | 28.8 | 312 | 181 | 8 | 90 | 362 | 183 | 492 | 1.7e-24 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94401.t1 | G7KAI8 | 93.1 | 391 | 22 | 1 | 1 | 391 | 12 | 397 | 2.1e-169 | 604.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060830 | MS.gene94401 | 0.800867 | 1.18E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene94401 | MS.gene003175 | PPI |
| MS.gene003846 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene88378 | PPI |
| MS.gene062301 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene28303 | PPI |
| MS.gene063568 | MS.gene94401 | PPI |
| MS.gene01984 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene65479 | PPI |
| MS.gene28304 | MS.gene94401 | PPI |
| MS.gene063209 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene038409 | PPI |
| MS.gene94401 | MS.gene45448 | PPI |
| MS.gene031356 | MS.gene94401 | PPI |
| MS.gene02854 | MS.gene94401 | PPI |
| MS.gene030707 | MS.gene94401 | PPI |
| MS.gene28161 | MS.gene94401 | PPI |
| MS.gene029825 | MS.gene94401 | PPI |
| MS.gene017174 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene056617 | PPI |
| MS.gene065496 | MS.gene94401 | PPI |
| MS.gene75013 | MS.gene94401 | PPI |
| MS.gene94401 | MS.gene58159 | PPI |
| MS.gene94401 | MS.gene048301 | PPI |
| MS.gene94401 | MS.gene053381 | PPI |
| MS.gene94401 | MS.gene70303 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94401.t1 | MTR_5g064850 | 97.024 | 336 | 10 | 0 | 56 | 391 | 62 | 397 | 0.0 | 681 |
| MS.gene94401.t1 | MTR_7g007890 | 96.450 | 338 | 12 | 0 | 54 | 391 | 60 | 397 | 0.0 | 677 |
| MS.gene94401.t1 | MTR_5g064850 | 96.726 | 336 | 9 | 1 | 56 | 391 | 62 | 395 | 0.0 | 673 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94401.t1 | AT5G61330 | 61.264 | 364 | 126 | 5 | 33 | 390 | 82 | 436 | 3.63e-143 | 414 |
| MS.gene94401.t1 | AT5G61330 | 61.264 | 364 | 126 | 5 | 33 | 390 | 82 | 436 | 3.63e-143 | 414 |
| MS.gene94401.t1 | AT5G61330 | 61.264 | 364 | 126 | 5 | 33 | 390 | 93 | 447 | 3.91e-143 | 414 |
Find 66 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 7.1:-86273741 | MS.gene94401:CDS |
| TTTAGAAGTTTCTAACAATT+TGG | 0.183126 | 7.1:-86274428 | MS.gene94401:CDS |
| TGAAAAGATAGTAAACTTTA+TGG | 0.190174 | 7.1:-86271238 | MS.gene94401:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.242515 | 7.1:-86273935 | MS.gene94401:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 7.1:-86276359 | MS.gene94401:CDS |
| AATCTCACAGCTCCTGTTCT+TGG | 0.278560 | 7.1:+86278545 | None:intergenic |
| CATGAAGAACTCACCATTAC+TGG | 0.321407 | 7.1:+86275711 | None:intergenic |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 7.1:+86273773 | None:intergenic |
| GAATTAGACGTGACAGTTAG+AGG | 0.346942 | 7.1:-86276412 | MS.gene94401:CDS |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 7.1:-86276371 | MS.gene94401:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 7.1:+86273802 | None:intergenic |
| TCAGAATCGTCTAGAAGTTC+AGG | 0.365325 | 7.1:+86272825 | None:intergenic |
| ATGAATTGGTACCTCGTTAT+AGG | 0.369864 | 7.1:+86278803 | None:intergenic |
| ATGCTATTTGATGATCTGTT+TGG | 0.374783 | 7.1:-86271180 | MS.gene94401:CDS |
| GAGGAGGAAGAGGAGGAAGA+TGG | 0.401220 | 7.1:-86278676 | MS.gene94401:CDS |
| GTTGAAGGAGGAGGAGGAAG+AGG | 0.405386 | 7.1:-86278686 | MS.gene94401:CDS |
| GGAGGAAGATGGAGAGGATG+AGG | 0.413025 | 7.1:-86278665 | MS.gene94401:CDS |
| ACAACTTGAGAAAGAGTATA+GGG | 0.419407 | 7.1:-86278578 | MS.gene94401:CDS |
| AAATGATGATAGCGAGTTGA+AGG | 0.429024 | 7.1:-86278701 | MS.gene94401:CDS |
| GCCATGGGAGGAAGATTTGC+CGG | 0.449865 | 7.1:+86271207 | None:intergenic |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 7.1:-86278602 | MS.gene94401:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 7.1:-86272768 | MS.gene94401:intron |
| GATGAGGGACAAGAGGAAAG+TGG | 0.454854 | 7.1:-86278622 | MS.gene94401:CDS |
| AGATTTCTCGGATGCACAAC+AGG | 0.459754 | 7.1:-86274378 | MS.gene94401:intron |
| AAGAGTGGTCACAGATTTCT+CGG | 0.468215 | 7.1:-86274390 | MS.gene94401:CDS |
| ATCAAATAGCATTGGCGCCA+TGG | 0.471359 | 7.1:+86271191 | None:intergenic |
| TGATGAGGAAGAAGAAGATG+AGG | 0.475179 | 7.1:-86278638 | MS.gene94401:CDS |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 7.1:+86276363 | None:intergenic |
| TAATGATAGCTCTAATGAAC+CGG | 0.480771 | 7.1:-86273613 | MS.gene94401:intron |
| CACAGCCAACCGTGATTATG+AGG | 0.481456 | 7.1:+86271128 | None:intergenic |
| TAGCGAGTTGAAGGAGGAGG+AGG | 0.487451 | 7.1:-86278692 | MS.gene94401:CDS |
| AGTCGATTTGAACTTGAGAA+TGG | 0.488419 | 7.1:+86276555 | None:intergenic |
| TGATGATAGCGAGTTGAAGG+AGG | 0.491832 | 7.1:-86278698 | MS.gene94401:CDS |
| GAGACAAGTCAATCAACAAA+TGG | 0.495909 | 7.1:-86273979 | MS.gene94401:CDS |
| AAACTTTATGGCTCCTCGAC+CGG | 0.496516 | 7.1:-86271226 | MS.gene94401:CDS |
| GGATCTTCATCACCAAGAAC+AGG | 0.499445 | 7.1:-86278557 | MS.gene94401:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 7.1:-86273953 | MS.gene94401:CDS |
| AGAAGAAGATGAGGGACAAG+AGG | 0.506023 | 7.1:-86278629 | MS.gene94401:CDS |
| GAGGAAGATGGAGAGGATGA+GGG | 0.507143 | 7.1:-86278664 | MS.gene94401:CDS |
| TGATAGCGAGTTGAAGGAGG+AGG | 0.507347 | 7.1:-86278695 | MS.gene94401:CDS |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 7.1:+86272759 | None:intergenic |
| AACAACTTGAGAAAGAGTAT+AGG | 0.516904 | 7.1:-86278579 | MS.gene94401:CDS |
| GAAGGAGGAGGAGGAAGAGG+AGG | 0.517844 | 7.1:-86278683 | MS.gene94401:CDS |
| ACCGGCAAATCTTCCTCCCA+TGG | 0.522237 | 7.1:-86271208 | MS.gene94401:CDS |
| GGAAGAGGAGGAAGATGGAG+AGG | 0.534241 | 7.1:-86278671 | MS.gene94401:CDS |
| TCAAATAGCATTGGCGCCAT+GGG | 0.534558 | 7.1:+86271192 | None:intergenic |
| GGAGGAAGATTTGCCGGTCG+AGG | 0.535642 | 7.1:+86271213 | None:intergenic |
| TTCTATATTGGAACTTCAAG+AGG | 0.536268 | 7.1:-86276347 | MS.gene94401:intron |
| AACAGATCATCAAATAGCAT+TGG | 0.546718 | 7.1:+86271183 | None:intergenic |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 7.1:+86273774 | None:intergenic |
| AGGGACAAGAGGAAAGTGGA+TGG | 0.557551 | 7.1:-86278618 | MS.gene94401:CDS |
| AGAGGATGAGGGAAGTGATG+AGG | 0.561924 | 7.1:-86278653 | MS.gene94401:CDS |
| TTACTGGCTTGAGCAACTGA+TGG | 0.562545 | 7.1:+86275727 | None:intergenic |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 7.1:-86276380 | MS.gene94401:CDS |
| GATGAGGAAGAAGAAGATGA+GGG | 0.565891 | 7.1:-86278637 | MS.gene94401:CDS |
| AAGTTCAAATCGACTACCCC+AGG | 0.575981 | 7.1:-86276547 | MS.gene94401:intron |
| TCAGTTGCTCAAGCCAGTAA+TGG | 0.587160 | 7.1:-86275724 | MS.gene94401:intron |
| CTTATATGTCCAGGTTGCCG+AGG | 0.602529 | 7.1:-86273637 | MS.gene94401:intron |
| AATAGCATTGGCGCCATGGG+AGG | 0.603352 | 7.1:+86271195 | None:intergenic |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 7.1:+86273620 | None:intergenic |
| AAAGATGAAGATCTCCTGAA+AGG | 0.616901 | 7.1:-86276827 | MS.gene94401:CDS |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 7.1:-86273973 | MS.gene94401:CDS |
| ATGACAATCTTGATCAAGAG+TGG | 0.634506 | 7.1:-86274405 | MS.gene94401:CDS |
| ACAAGCTGTGAAAAGCCAGA+AGG | 0.675511 | 7.1:-86276804 | MS.gene94401:intron |
| TGATGATATGTCCTATAACG+AGG | 0.731413 | 7.1:-86278814 | MS.gene94401:intron |
| GCTCAGGAAACACATGCTGA+CGG | 0.737841 | 7.1:-86272852 | MS.gene94401:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 86271143 | 86278874 | 86271143 | ID=MS.gene94401 |
| chr7.1 | mRNA | 86271143 | 86278874 | 86271143 | ID=MS.gene94401.t1;Parent=MS.gene94401 |
| chr7.1 | exon | 86278815 | 86278874 | 86278815 | ID=MS.gene94401.t1.exon1;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86278815 | 86278874 | 86278815 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86278553 | 86278725 | 86278553 | ID=MS.gene94401.t1.exon2;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86278553 | 86278725 | 86278553 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86276805 | 86276877 | 86276805 | ID=MS.gene94401.t1.exon3;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86276805 | 86276877 | 86276805 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86276548 | 86276622 | 86276548 | ID=MS.gene94401.t1.exon4;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86276548 | 86276622 | 86276548 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86276348 | 86276458 | 86276348 | ID=MS.gene94401.t1.exon5;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86276348 | 86276458 | 86276348 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86275725 | 86275767 | 86275725 | ID=MS.gene94401.t1.exon6;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86275725 | 86275767 | 86275725 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86274379 | 86274472 | 86274379 | ID=MS.gene94401.t1.exon7;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86274379 | 86274472 | 86274379 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86273913 | 86274015 | 86273913 | ID=MS.gene94401.t1.exon8;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86273913 | 86274015 | 86273913 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86273737 | 86273832 | 86273737 | ID=MS.gene94401.t1.exon9;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86273737 | 86273832 | 86273737 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86273614 | 86273646 | 86273614 | ID=MS.gene94401.t1.exon10;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86273614 | 86273646 | 86273614 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86272769 | 86272868 | 86272769 | ID=MS.gene94401.t1.exon11;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86272769 | 86272868 | 86272769 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86271370 | 86271454 | 86271370 | ID=MS.gene94401.t1.exon12;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86271370 | 86271454 | 86271370 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| chr7.1 | exon | 86271143 | 86271272 | 86271143 | ID=MS.gene94401.t1.exon13;Parent=MS.gene94401.t1 |
| chr7.1 | CDS | 86271143 | 86271272 | 86271143 | ID=cds.MS.gene94401.t1;Parent=MS.gene94401.t1 |
| Gene Sequence |
| Protein sequence |
