Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95340.t1 | PNX91497.1 | 84.2 | 184 | 27 | 1 | 1 | 182 | 1 | 184 | 3.40E-78 | 301.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95340.t1 | P10978 | 35.7 | 168 | 105 | 2 | 7 | 171 | 5 | 172 | 1.1e-24 | 115.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95340.t1 | A0A2K3ML10 | 84.2 | 184 | 27 | 1 | 1 | 182 | 1 | 184 | 2.5e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene95340 | 0.844035 | 9.70E-59 | -1.69E-46 |
| MS.gene049983 | MS.gene95340 | 0.820427 | 6.90E-53 | -1.69E-46 |
| MS.gene050085 | MS.gene95340 | 0.801828 | 7.49E-49 | -1.69E-46 |
| MS.gene050213 | MS.gene95340 | 0.822838 | 1.91E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene95340 | 0.826471 | 2.66E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene95340 | 0.840185 | 1.01E-57 | -1.69E-46 |
| MS.gene050941 | MS.gene95340 | 0.816115 | 6.53E-52 | -1.69E-46 |
| MS.gene051275 | MS.gene95340 | 0.829741 | 4.34E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene95340 | 0.834746 | 2.51E-56 | -1.69E-46 |
| MS.gene052307 | MS.gene95340 | 0.815478 | 9.06E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene95340 | 0.812945 | 3.28E-51 | -1.69E-46 |
| MS.gene052776 | MS.gene95340 | 0.815467 | 9.11E-52 | -1.69E-46 |
| MS.gene053726 | MS.gene95340 | 0.822983 | 1.77E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene95340 | 0.809264 | 2.06E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene95340 | 0.815845 | 7.50E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene95340 | 0.820774 | 5.74E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene95340 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene95340 | 0.828158 | 1.05E-54 | -1.69E-46 |
| MS.gene05615 | MS.gene95340 | 0.837449 | 5.16E-57 | -1.69E-46 |
| MS.gene056181 | MS.gene95340 | 0.810227 | 1.28E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene95340 | 0.80046 | 1.43E-48 | -1.69E-46 |
| MS.gene057208 | MS.gene95340 | 0.802738 | 4.86E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene95340 | -0.811915 | 5.51E-51 | -1.69E-46 |
| MS.gene058081 | MS.gene95340 | 0.814637 | 1.39E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene95340 | 0.82608 | 3.30E-54 | -1.69E-46 |
| MS.gene058429 | MS.gene95340 | 0.805441 | 1.33E-49 | -1.69E-46 |
| MS.gene059007 | MS.gene95340 | 0.80777 | 4.30E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene95340 | 0.803183 | 3.94E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene95340 | 0.811009 | 8.67E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene95340 | 0.815362 | 9.61E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene95340 | 0.814918 | 1.21E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene95340 | 0.815304 | 9.90E-52 | -1.69E-46 |
| MS.gene06121 | MS.gene95340 | 0.80419 | 2.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95340.t1 | MTR_2g039140 | 38.415 | 164 | 68 | 3 | 1 | 163 | 120 | 251 | 7.45e-24 | 97.4 |
| MS.gene95340.t1 | MTR_4g036430 | 89.744 | 39 | 4 | 0 | 1 | 39 | 31 | 69 | 1.66e-19 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95340.t1 | AT3G29785 | 57.778 | 90 | 37 | 1 | 11 | 100 | 7 | 95 | 8.08e-32 | 113 |
Find 54 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATACCAACTGAACTTAAT+TGG | 0.095422 | 1.3:+18035457 | None:intergenic |
| CTGAGAACTACGATGATTTC+TGG | 0.189947 | 1.3:+18035173 | None:intergenic |
| TCATCATCCTTCATCGATTC+TGG | 0.247368 | 1.3:+18035717 | None:intergenic |
| TTCGCCTCTTGATTCTTTGC+TGG | 0.253882 | 1.3:+18035084 | None:intergenic |
| ACTACGATGATTTCTGGATT+TGG | 0.314338 | 1.3:+18035179 | None:intergenic |
| TCGCCTCTTGATTCTTTGCT+GGG | 0.374564 | 1.3:+18035085 | None:intergenic |
| ATGGTGCGGACTTTGGCTTT+TGG | 0.377376 | 1.3:-18035803 | MS.gene95340:CDS |
| GATACCAACTGAACTTAATT+GGG | 0.381678 | 1.3:+18035458 | None:intergenic |
| ACAGTAGCACTCCAACTATC+TGG | 0.413891 | 1.3:+18035390 | None:intergenic |
| AGAAATTCAACCAGAGGAAA+TGG | 0.417568 | 1.3:-18035228 | MS.gene95340:CDS |
| TGTCTTCCCTACCAGATAGT+TGG | 0.424640 | 1.3:-18035401 | MS.gene95340:CDS |
| GAGATCCGACGGAGAGAGTT+GGG | 0.442215 | 1.3:-18035294 | MS.gene95340:CDS |
| GCAAATGTTGCTTCCACCTC+AGG | 0.445655 | 1.3:-18035054 | MS.gene95340:CDS |
| AAGTTCGATGGTGCGGACTT+TGG | 0.452497 | 1.3:-18035810 | MS.gene95340:CDS |
| TGAACTTTATTTGATGCTGA+AGG | 0.474628 | 1.3:+18035564 | None:intergenic |
| TTTACCACGTCCATTTCCTC+TGG | 0.487326 | 1.3:+18035218 | None:intergenic |
| TGAGGCGGTTGTTTACGCTT+CGG | 0.506448 | 1.3:-18035536 | MS.gene95340:CDS |
| TCTATCGAGTGTTGGAATTG+TGG | 0.512167 | 1.3:-18035141 | MS.gene95340:CDS |
| TGTTGGAATTGTGGAAAGAC+AGG | 0.522152 | 1.3:-18035132 | MS.gene95340:CDS |
| AAAGAAACCAGAATCGATGA+AGG | 0.539324 | 1.3:-18035724 | MS.gene95340:CDS |
| CTCTGTATGCAATACTGAAG+AGG | 0.540619 | 1.3:+18035260 | None:intergenic |
| TGCGTCACCTCCTCCTGAGG+TGG | 0.540756 | 1.3:+18035041 | None:intergenic |
| AGAGATCCGACGGAGAGAGT+TGG | 0.549236 | 1.3:-18035295 | MS.gene95340:CDS |
| CAAATAAAGTTCATTTGATG+AGG | 0.550433 | 1.3:-18035554 | MS.gene95340:CDS |
| ACTCAAAATCTATCGAGTGT+TGG | 0.554206 | 1.3:-18035149 | MS.gene95340:CDS |
| AACCACCGCTGGTCTTATGA+AGG | 0.557775 | 1.3:-18035610 | MS.gene95340:CDS |
| TAGCACTCCAACTATCTGGT+AGG | 0.558134 | 1.3:+18035394 | None:intergenic |
| TTTACGCTTCGGATGACAGA+AGG | 0.562705 | 1.3:-18035525 | MS.gene95340:CDS |
| GTGAAAATTGAAAAGTTCGA+TGG | 0.563926 | 1.3:-18035822 | MS.gene95340:CDS |
| ACAACCCAATTAAGTTCAGT+TGG | 0.568124 | 1.3:-18035462 | MS.gene95340:CDS |
| GTTGCTTCCACCTCAGGAGG+AGG | 0.568689 | 1.3:-18035048 | MS.gene95340:CDS |
| GATGACAGAAGGCGCATCAG+TGG | 0.593273 | 1.3:-18035514 | MS.gene95340:CDS |
| TTCTCAGTGAAGAGATCCGA+CGG | 0.596721 | 1.3:-18035305 | MS.gene95340:intron |
| AGCACTCCAACTATCTGGTA+GGG | 0.597976 | 1.3:+18035395 | None:intergenic |
| ATAAAGTTCATTTGATGAGG+CGG | 0.598437 | 1.3:-18035551 | MS.gene95340:CDS |
| AAATGGACGTGGTAAATCAA+AGG | 0.601633 | 1.3:-18035211 | MS.gene95340:CDS |
| CTTCTCGATAGACAAGCACT+TGG | 0.601850 | 1.3:-18035687 | MS.gene95340:CDS |
| AATGTTGCTTCCACCTCAGG+AGG | 0.603928 | 1.3:-18035051 | MS.gene95340:CDS |
| AATCGATGAAGGATGATGAG+TGG | 0.606445 | 1.3:-18035713 | MS.gene95340:CDS |
| AATTGAAAAGTTCGATGGTG+CGG | 0.608192 | 1.3:-18035817 | MS.gene95340:CDS |
| CAAGTGCTTGTCTATCGAGA+AGG | 0.612004 | 1.3:+18035688 | None:intergenic |
| TATGGCTGCAGACGAAGGAA+AGG | 0.614378 | 1.3:-18035844 | MS.gene95340:CDS |
| GGAGTTATGGCTGCAGACGA+AGG | 0.617375 | 1.3:-18035849 | None:intergenic |
| ATTCACCCAACTCTCTCCGT+CGG | 0.618585 | 1.3:+18035289 | None:intergenic |
| AGCCTTCATAAGACCAGCGG+TGG | 0.620835 | 1.3:+18035608 | None:intergenic |
| GAATCAAGAGGCGAAAGATG+AGG | 0.623736 | 1.3:-18035076 | MS.gene95340:CDS |
| GCAAAAGAGAAAACCACCGC+TGG | 0.626545 | 1.3:-18035621 | MS.gene95340:CDS |
| CAATGCGTCACCTCCTCCTG+AGG | 0.628987 | 1.3:+18035038 | None:intergenic |
| GTATTGCATACAGAGTAAAG+AGG | 0.635010 | 1.3:-18035252 | MS.gene95340:CDS |
| TGGTATCGAATTTGACGATG+AGG | 0.639552 | 1.3:-18035442 | MS.gene95340:CDS |
| AGAGGAAGAAATTCAACCAG+AGG | 0.648533 | 1.3:-18035234 | MS.gene95340:CDS |
| TCAACCAGAGGAAATGGACG+TGG | 0.665050 | 1.3:-18035222 | MS.gene95340:CDS |
| GCTCCCAGCAAAGAATCAAG+AGG | 0.671318 | 1.3:-18035088 | MS.gene95340:CDS |
| AAGAGCCTTCATAAGACCAG+CGG | 0.707222 | 1.3:+18035605 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAATAAAGTTCATTTGATG+AGG | - | chr1.3:18035326-18035345 | MS.gene95340:intron | 25.0% |
| ATAAAGTTCATTTGATGAGG+CGG | - | chr1.3:18035329-18035348 | MS.gene95340:intron | 30.0% | |
| GATACCAACTGAACTTAATT+GGG | + | chr1.3:18035425-18035444 | None:intergenic | 30.0% | |
| GTGAAAATTGAAAAGTTCGA+TGG | - | chr1.3:18035058-18035077 | MS.gene95340:CDS | 30.0% | |
| ! | TGAACTTTATTTGATGCTGA+AGG | + | chr1.3:18035319-18035338 | None:intergenic | 30.0% |
| AAAGAAACCAGAATCGATGA+AGG | - | chr1.3:18035156-18035175 | MS.gene95340:CDS | 35.0% | |
| AAATGGACGTGGTAAATCAA+AGG | - | chr1.3:18035669-18035688 | MS.gene95340:CDS | 35.0% | |
| AATTGAAAAGTTCGATGGTG+CGG | - | chr1.3:18035063-18035082 | MS.gene95340:CDS | 35.0% | |
| ACAACCCAATTAAGTTCAGT+TGG | - | chr1.3:18035418-18035437 | MS.gene95340:CDS | 35.0% | |
| ACTCAAAATCTATCGAGTGT+TGG | - | chr1.3:18035731-18035750 | MS.gene95340:CDS | 35.0% | |
| AGAAATTCAACCAGAGGAAA+TGG | - | chr1.3:18035652-18035671 | MS.gene95340:CDS | 35.0% | |
| CGATACCAACTGAACTTAAT+TGG | + | chr1.3:18035426-18035445 | None:intergenic | 35.0% | |
| GACACTTCAAAAATCAGTGT+AGG | - | chr1.3:18035770-18035789 | MS.gene95340:CDS | 35.0% | |
| GTATTGCATACAGAGTAAAG+AGG | - | chr1.3:18035628-18035647 | MS.gene95340:CDS | 35.0% | |
| ! | ACTACGATGATTTCTGGATT+TGG | + | chr1.3:18035704-18035723 | None:intergenic | 35.0% |
| AATCGATGAAGGATGATGAG+TGG | - | chr1.3:18035167-18035186 | MS.gene95340:CDS | 40.0% | |
| AGAGGAAGAAATTCAACCAG+AGG | - | chr1.3:18035646-18035665 | MS.gene95340:CDS | 40.0% | |
| ATTTGACGATGTTCGTGATC+TGG | - | chr1.3:18035552-18035571 | MS.gene95340:CDS | 40.0% | |
| CTCTGTATGCAATACTGAAG+AGG | + | chr1.3:18035623-18035642 | None:intergenic | 40.0% | |
| CTGAGAACTACGATGATTTC+TGG | + | chr1.3:18035710-18035729 | None:intergenic | 40.0% | |
| TGTTGGAATTGTGGAAAGAC+AGG | - | chr1.3:18035748-18035767 | MS.gene95340:CDS | 40.0% | |
| ! | TGGTATCGAATTTGACGATG+AGG | - | chr1.3:18035438-18035457 | MS.gene95340:CDS | 40.0% |
| ! | TTTCATCTTTTTGCTTCCCG+AGG | + | chr1.3:18035533-18035552 | None:intergenic | 40.0% |
| !! | TCATCATCCTTCATCGATTC+TGG | + | chr1.3:18035166-18035185 | None:intergenic | 40.0% |
| !! | TCTATCGAGTGTTGGAATTG+TGG | - | chr1.3:18035739-18035758 | MS.gene95340:CDS | 40.0% |
| !! | TCTGGTTTCTTTTCTGTGAG+AGG | + | chr1.3:18035148-18035167 | None:intergenic | 40.0% |
| AAGAGCCTTCATAAGACCAG+CGG | + | chr1.3:18035278-18035297 | None:intergenic | 45.0% | |
| ACAGTAGCACTCCAACTATC+TGG | + | chr1.3:18035493-18035512 | None:intergenic | 45.0% | |
| AGCACTCCAACTATCTGGTA+GGG | + | chr1.3:18035488-18035507 | None:intergenic | 45.0% | |
| CAAGTGCTTGTCTATCGAGA+AGG | + | chr1.3:18035195-18035214 | None:intergenic | 45.0% | |
| CTTCTCGATAGACAAGCACT+TGG | - | chr1.3:18035193-18035212 | MS.gene95340:CDS | 45.0% | |
| GAATCAAGAGGCGAAAGATG+AGG | - | chr1.3:18035804-18035823 | MS.gene95340:CDS | 45.0% | |
| TAGCACTCCAACTATCTGGT+AGG | + | chr1.3:18035489-18035508 | None:intergenic | 45.0% | |
| TCGCCTCTTGATTCTTTGCT+GGG | + | chr1.3:18035798-18035817 | None:intergenic | 45.0% | |
| TGTCTTCCCTACCAGATAGT+TGG | - | chr1.3:18035479-18035498 | MS.gene95340:CDS | 45.0% | |
| TTCGCCTCTTGATTCTTTGC+TGG | + | chr1.3:18035799-18035818 | None:intergenic | 45.0% | |
| TTCTCAGTGAAGAGATCCGA+CGG | - | chr1.3:18035575-18035594 | MS.gene95340:CDS | 45.0% | |
| TTTACCACGTCCATTTCCTC+TGG | + | chr1.3:18035665-18035684 | None:intergenic | 45.0% | |
| TTTACGCTTCGGATGACAGA+AGG | - | chr1.3:18035355-18035374 | MS.gene95340:intron | 45.0% | |
| AAGTTCGATGGTGCGGACTT+TGG | - | chr1.3:18035070-18035089 | MS.gene95340:CDS | 50.0% | |
| ATTCACCCAACTCTCTCCGT+CGG | + | chr1.3:18035594-18035613 | None:intergenic | 50.0% | |
| GCAAAAGAGAAAACCACCGC+TGG | - | chr1.3:18035259-18035278 | MS.gene95340:CDS | 50.0% | |
| GCTCCCAGCAAAGAATCAAG+AGG | - | chr1.3:18035792-18035811 | MS.gene95340:CDS | 50.0% | |
| TCAACCAGAGGAAATGGACG+TGG | - | chr1.3:18035658-18035677 | MS.gene95340:CDS | 50.0% | |
| TGAGGCGGTTGTTTACGCTT+CGG | - | chr1.3:18035344-18035363 | MS.gene95340:intron | 50.0% | |
| ! | AATGTTGCTTCCACCTCAGG+AGG | - | chr1.3:18035829-18035848 | MS.gene95340:CDS | 50.0% |
| ! | GCAAATGTTGCTTCCACCTC+AGG | - | chr1.3:18035826-18035845 | MS.gene95340:CDS | 50.0% |
| !! | AACCACCGCTGGTCTTATGA+AGG | - | chr1.3:18035270-18035289 | MS.gene95340:CDS | 50.0% |
| !!! | ATGGTGCGGACTTTGGCTTT+TGG | - | chr1.3:18035077-18035096 | MS.gene95340:CDS | 50.0% |
| ACAGCTGTGAGTAGCTCCTC+GGG | - | chr1.3:18035514-18035533 | MS.gene95340:CDS | 55.0% | |
| AGAGATCCGACGGAGAGAGT+TGG | - | chr1.3:18035585-18035604 | MS.gene95340:CDS | 55.0% | |
| AGCCTTCATAAGACCAGCGG+TGG | + | chr1.3:18035275-18035294 | None:intergenic | 55.0% | |
| CACAGCTGTGAGTAGCTCCT+CGG | - | chr1.3:18035513-18035532 | MS.gene95340:CDS | 55.0% | |
| GAGATCCGACGGAGAGAGTT+GGG | - | chr1.3:18035586-18035605 | MS.gene95340:CDS | 55.0% | |
| ! | GATGACAGAAGGCGCATCAG+TGG | - | chr1.3:18035366-18035385 | MS.gene95340:intron | 55.0% |
| CAATGCGTCACCTCCTCCTG+AGG | + | chr1.3:18035845-18035864 | None:intergenic | 60.0% | |
| GTTGCTTCCACCTCAGGAGG+AGG | - | chr1.3:18035832-18035851 | MS.gene95340:CDS | 60.0% | |
| TGCGTCACCTCCTCCTGAGG+TGG | + | chr1.3:18035842-18035861 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 18035037 | 18035865 | 18035037 | ID=MS.gene95340 |
| chr1.3 | mRNA | 18035037 | 18035865 | 18035037 | ID=MS.gene95340.t1;Parent=MS.gene95340 |
| chr1.3 | exon | 18035383 | 18035865 | 18035383 | ID=MS.gene95340.t1.exon1;Parent=MS.gene95340.t1 |
| chr1.3 | CDS | 18035383 | 18035865 | 18035383 | ID=cds.MS.gene95340.t1;Parent=MS.gene95340.t1 |
| chr1.3 | exon | 18035037 | 18035315 | 18035037 | ID=MS.gene95340.t1.exon2;Parent=MS.gene95340.t1 |
| chr1.3 | CDS | 18035037 | 18035315 | 18035037 | ID=cds.MS.gene95340.t1;Parent=MS.gene95340.t1 |
| Gene Sequence |
| Protein sequence |
