AlfalfaGEDB | MS.gene95552

M. sativa cultivar XinJiangDaYe / MS.gene95552

KEGG: K03327
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95552.t1	XP_024638064.1	96.7	120	4	0	1	120	1	120	1.40E-56	228.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95552.t1	Q9LVD9	61.8	110	31	2	11	120	9	107	1.2e-27	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95552.t1	G7JDH9	96.7	120	4	0	1	120	1	120	1.0e-56	228.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050680	MS.gene95552	0.829775	4.26E-55	-1.69E-46
MS.gene050750	MS.gene95552	0.808601	2.86E-50	-1.69E-46
MS.gene051368	MS.gene95552	0.83313	6.36E-56	-1.69E-46
MS.gene051568	MS.gene95552	0.803554	3.30E-49	-1.69E-46
MS.gene052190	MS.gene95552	0.826179	3.13E-54	-1.69E-46
MS.gene052333	MS.gene95552	0.881612	2.08E-70	-1.69E-46
MS.gene053166	MS.gene95552	0.845912	3.02E-59	-1.69E-46
MS.gene053584	MS.gene95552	0.806021	1.01E-49	-1.69E-46
MS.gene054183	MS.gene95552	0.800956	1.13E-48	-1.69E-46
MS.gene054291	MS.gene95552	-0.801055	1.08E-48	-1.69E-46
MS.gene054468	MS.gene95552	0.807496	4.91E-50	-1.69E-46
MS.gene054559	MS.gene95552	0.849011	4.25E-60	-1.69E-46
MS.gene055356	MS.gene95552	0.83861	2.60E-57	-1.69E-46
MS.gene055358	MS.gene95552	0.829431	5.17E-55	-1.69E-46
MS.gene055367	MS.gene95552	0.840959	6.35E-58	-1.69E-46
MS.gene055701	MS.gene95552	0.851626	7.85E-61	-1.69E-46
MS.gene05595	MS.gene95552	0.840318	9.35E-58	-1.69E-46
MS.gene056506	MS.gene95552	0.803899	2.80E-49	-1.69E-46
MS.gene057156	MS.gene95552	-0.829879	4.02E-55	-1.69E-46
MS.gene058935	MS.gene95552	0.829622	4.64E-55	-1.69E-46
MS.gene059128	MS.gene95552	0.832387	9.73E-56	-1.69E-46
MS.gene059161	MS.gene95552	0.803029	4.24E-49	-1.69E-46
MS.gene059306	MS.gene95552	0.804841	1.78E-49	-1.69E-46
MS.gene060011	MS.gene95552	0.830075	3.60E-55	-1.69E-46
MS.gene061114	MS.gene95552	-0.833006	6.83E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95552.t1	MTR_4g048000	96.667	120	4	0	1	120	1	120	1.05e-78	241
MS.gene95552.t1	MTR_2g088400	70.435	115	20	2	8	120	6	108	2.55e-46	155
MS.gene95552.t1	MTR_2g088400	70.435	115	20	2	8	120	6	108	7.08e-46	155
MS.gene95552.t1	MTR_2g022980	56.122	98	39	1	23	120	11	104	4.75e-28	107
MS.gene95552.t1	MTR_5g090290	36.275	102	63	2	19	120	3	102	1.03e-13	66.6
MS.gene95552.t1	MTR_5g032720	37.500	88	46	1	42	120	5	92	2.31e-12	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95552.t1	AT3G21690	75.610	82	20	0	39	120	26	107	6.80e-36	128
MS.gene95552.t1	AT1G61890	53.488	86	40	0	35	120	17	102	5.59e-24	95.5
MS.gene95552.t1	AT1G61890	53.488	86	40	0	35	120	17	102	7.00e-24	95.5
MS.gene95552.t1	AT1G61890	53.488	86	40	0	35	120	17	102	7.64e-24	95.5
MS.gene95552.t1	AT1G11670	52.326	86	41	0	35	120	20	105	1.42e-23	94.7
MS.gene95552.t1	AT4G21910	50.000	78	38	1	43	120	35	111	4.95e-18	79.0
MS.gene95552.t1	AT4G21910	50.000	78	38	1	43	120	35	111	4.95e-18	79.0
MS.gene95552.t1	AT4G21910	43.590	78	43	1	43	120	33	109	2.44e-16	74.3
MS.gene95552.t1	AT4G21910	43.590	78	43	1	43	120	33	109	2.44e-16	74.3
MS.gene95552.t1	AT3G59030	39.535	86	49	2	37	120	22	106	4.90e-12	61.6
MS.gene95552.t1	AT3G59030	40.476	84	47	2	39	120	24	106	1.18e-11	60.8

Find 20 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTGGTCATCTTGGTAATT+TGG	0.143264	4.3:+50943208	MS.gene95552:CDS
GGTATCCAAATCTTTGCTTA+TGG	0.262856	4.3:+50943260	MS.gene95552:CDS
GCTGCTTCCCTTGGAAATAA+TGG	0.345684	4.3:+50943239	MS.gene95552:CDS
TCATCATTCACTCATTCATT+TGG	0.367294	4.3:+50943008	MS.gene95552:CDS
GAGCTTGCTGCTGCTTCCCT+TGG	0.368536	4.3:+50943230	MS.gene95552:CDS
TGAGTCCATAAGCAAAGATT+TGG	0.378864	4.3:-50943265	None:intergenic
AGTATTGGTGTTGGTTCACT+TGG	0.433830	4.3:-50942981	None:intergenic
TGTCTAATACGTTTCACAAA+AGG	0.464084	4.3:-50943086	None:intergenic
TTTGGATACCATTATTTCCA+AGG	0.506390	4.3:-50943247	None:intergenic
AATCTTTGCTTATGGACTCA+TGG	0.512650	4.3:+50943268	MS.gene95552:CDS
TTGACATCACATAATTGATA+AGG	0.514223	4.3:-50943169	None:intergenic
GGTTCACTTGGTACTAGCAA+AGG	0.520440	4.3:-50942969	None:intergenic
AACGTATTAGACATGCTACA+TGG	0.535997	4.3:+50943096	MS.gene95552:CDS
GTGAATGATGATAGTAGTAT+TGG	0.560516	4.3:-50942996	None:intergenic
GGTTCAAAGCATGAATCAGA+TGG	0.585307	4.3:+50943029	MS.gene95552:CDS
TTGGATACCATTATTTCCAA+GGG	0.585736	4.3:-50943246	None:intergenic
GATGATAGTAGTATTGGTGT+TGG	0.589523	4.3:-50942990	None:intergenic
CAATTACCGCAGGTGCAGCT+AGG	0.592518	4.3:-50943142	None:intergenic
ACGTATTAGACATGCTACAT+GGG	0.638198	4.3:+50943097	MS.gene95552:CDS
ATAAGGTACACAATTACCGC+AGG	0.652901	4.3:-50943152	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGACATCACATAATTGATA+AGG	-	chr4.3:50943172-50943191	None:intergenic	25.0%
!!	ATGTCTACTCAAATATTTTC+TGG	+	chr4.3:50943191-50943210	MS.gene95552:CDS	25.0%
	GTGAATGATGATAGTAGTAT+TGG	-	chr4.3:50942999-50943018	None:intergenic	30.0%
	TCATCATTCACTCATTCATT+TGG	+	chr4.3:50943008-50943027	MS.gene95552:CDS	30.0%
	TGTCTAATACGTTTCACAAA+AGG	-	chr4.3:50943089-50943108	None:intergenic	30.0%
	TTGGATACCATTATTTCCAA+GGG	-	chr4.3:50943249-50943268	None:intergenic	30.0%
	TTTGGATACCATTATTTCCA+AGG	-	chr4.3:50943250-50943269	None:intergenic	30.0%
!!	CAAATATTTTCTGGTCATCT+TGG	+	chr4.3:50943200-50943219	MS.gene95552:CDS	30.0%
!!!	ATTCTCTCTGTTTTAGTACT+TGG	+	chr4.3:50943304-50943323	MS.gene95552:intron	30.0%
	AACGTATTAGACATGCTACA+TGG	+	chr4.3:50943096-50943115	MS.gene95552:CDS	35.0%
	AATCTTTGCTTATGGACTCA+TGG	+	chr4.3:50943268-50943287	MS.gene95552:CDS	35.0%
	ACGTATTAGACATGCTACAT+GGG	+	chr4.3:50943097-50943116	MS.gene95552:CDS	35.0%
	GGTATCCAAATCTTTGCTTA+TGG	+	chr4.3:50943260-50943279	MS.gene95552:CDS	35.0%
	TGAGTCCATAAGCAAAGATT+TGG	-	chr4.3:50943268-50943287	None:intergenic	35.0%
!	TTCTGGTCATCTTGGTAATT+TGG	+	chr4.3:50943208-50943227	MS.gene95552:CDS	35.0%
!!	GATGATAGTAGTATTGGTGT+TGG	-	chr4.3:50942993-50943012	None:intergenic	35.0%
	ATAAGGTACACAATTACCGC+AGG	-	chr4.3:50943155-50943174	None:intergenic	40.0%
	GGTTCAAAGCATGAATCAGA+TGG	+	chr4.3:50943029-50943048	MS.gene95552:CDS	40.0%
!!	AGTATTGGTGTTGGTTCACT+TGG	-	chr4.3:50942984-50943003	None:intergenic	40.0%
	GCTGCTTCCCTTGGAAATAA+TGG	+	chr4.3:50943239-50943258	MS.gene95552:CDS	45.0%
	GGTTCACTTGGTACTAGCAA+AGG	-	chr4.3:50942972-50942991	None:intergenic	45.0%
!	TTTTTACCTAGCTGCACCTG+CGG	+	chr4.3:50943136-50943155	MS.gene95552:CDS	45.0%
	CAATTACCGCAGGTGCAGCT+AGG	-	chr4.3:50943145-50943164	None:intergenic	55.0%
!!	GAGCTTGCTGCTGCTTCCCT+TGG	+	chr4.3:50943230-50943249	MS.gene95552:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	50942930	50943337	50942930	ID=MS.gene95552
chr4.3	mRNA	50942930	50943337	50942930	ID=MS.gene95552.t1;Parent=MS.gene95552
chr4.3	exon	50942930	50943289	50942930	ID=MS.gene95552.t1.exon1;Parent=MS.gene95552.t1
chr4.3	CDS	50942930	50943289	50942930	ID=cds.MS.gene95552.t1;Parent=MS.gene95552.t1
chr4.3	exon	50943320	50943337	50943320	ID=MS.gene95552.t1.exon2;Parent=MS.gene95552.t1
chr4.3	CDS	50943320	50943337	50943320	ID=cds.MS.gene95552.t1;Parent=MS.gene95552.t1

Gene Sequence

>MS.gene95552

ATGGCTACTTCTACTCATGTTCACAAAGATATTGATGAACCTTTGCTAGTACCAAGTGAACCAACACCAATACTACTATCATCATTCACTCATTCATTTGGTTCAAAGCATGAATCAGATGGTGAACTTGAACGAATACTCTCAGATACAAGTGTTCCTTTTGTGAAACGTATTAGACATGCTACATGGGTTGAATTTAAGCTACTTTTTTACCTAGCTGCACCTGCGGTAATTGTGTACCTTATCAATTATGTGATGTCAATGTCTACTCAAATATTTTCTGGTCATCTTGGTAATTTGGAGCTTGCTGCTGCTTCCCTTGGAAATAATGGTATCCAAATCTTTGCTTATGGACTCATGGTATGAATCACTCTATTCTCTCTGTTTTAGTACTTGGATCTTCATTAA

Protein sequence

>MS.gene95552.t1

MATSTHVHKDIDEPLLVPSEPTPILLSSFTHSFGSKHESDGELERILSDTSVPFVKRIRHATWVEFKLLFYLAAPAVIVYLINYVMSMSTQIFSGHLGNLELAAASLGNNGIQIFAYGLMYLDLH