Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95714.t1 | KEH29628.1 | 96.4 | 110 | 4 | 0 | 1 | 110 | 1 | 110 | 1.10E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95714.t1 | A0A072UKK4 | 96.4 | 110 | 4 | 0 | 1 | 110 | 1 | 110 | 7.6e-48 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene95714 | 0.839993 | 1.14E-57 | -1.69E-46 |
| MS.gene050883 | MS.gene95714 | 0.824352 | 8.45E-54 | -1.69E-46 |
| MS.gene051959 | MS.gene95714 | 0.819832 | 9.44E-53 | -1.69E-46 |
| MS.gene051960 | MS.gene95714 | 0.820359 | 7.15E-53 | -1.69E-46 |
| MS.gene052053 | MS.gene95714 | 0.867181 | 1.65E-65 | -1.69E-46 |
| MS.gene052089 | MS.gene95714 | 0.818004 | 2.46E-52 | -1.69E-46 |
| MS.gene052090 | MS.gene95714 | 0.804044 | 2.61E-49 | -1.69E-46 |
| MS.gene052091 | MS.gene95714 | 0.863312 | 2.72E-64 | -1.69E-46 |
| MS.gene053932 | MS.gene95714 | 0.829577 | 4.76E-55 | -1.69E-46 |
| MS.gene055150 | MS.gene95714 | 0.814775 | 1.30E-51 | -1.69E-46 |
| MS.gene055549 | MS.gene95714 | 0.888558 | 5.31E-73 | -1.69E-46 |
| MS.gene055550 | MS.gene95714 | 0.891525 | 3.67E-74 | -1.69E-46 |
| MS.gene055551 | MS.gene95714 | 0.869296 | 3.44E-66 | -1.69E-46 |
| MS.gene056311 | MS.gene95714 | 0.814282 | 1.67E-51 | -1.69E-46 |
| MS.gene056365 | MS.gene95714 | 0.854501 | 1.18E-61 | -1.69E-46 |
| MS.gene056530 | MS.gene95714 | 0.873603 | 1.29E-67 | -1.69E-46 |
| MS.gene057001 | MS.gene95714 | 0.802152 | 6.42E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene95714 | 0.821447 | 4.02E-53 | -1.69E-46 |
| MS.gene058494 | MS.gene95714 | 0.814966 | 1.18E-51 | -1.69E-46 |
| MS.gene058628 | MS.gene95714 | 0.832743 | 7.94E-56 | -1.69E-46 |
| MS.gene058629 | MS.gene95714 | 0.815061 | 1.12E-51 | -1.69E-46 |
| MS.gene058630 | MS.gene95714 | 0.824903 | 6.27E-54 | -1.69E-46 |
| MS.gene05884 | MS.gene95714 | 0.82918 | 5.95E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95714.t1 | MTR_4g046147 | 96.364 | 110 | 4 | 0 | 1 | 110 | 1 | 110 | 4.30e-72 | 210 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGCAACCCTCTTTATCTT+AGG | 0.308528 | 4.1:-55073219 | None:intergenic |
| GATAAAGAGGGTTGCTGTTA+AGG | 0.330238 | 4.1:+55073224 | MS.gene95714:CDS |
| AGGCAAGGATATGAGCTTCT+TGG | 0.382110 | 4.1:+55073069 | MS.gene95714:CDS |
| CTAAAAGACCTAAGATAAAG+AGG | 0.402553 | 4.1:+55073211 | MS.gene95714:CDS |
| CTAGCTCCCCTCTTCCTACT+AGG | 0.437842 | 4.1:+55073328 | MS.gene95714:CDS |
| GTTGCGAACAAGTGTGTTCA+TGG | 0.455774 | 4.1:-55073025 | None:intergenic |
| TATGAGCTTCTTGGTTTACA+AGG | 0.464864 | 4.1:+55073078 | MS.gene95714:CDS |
| CCTACTAGGAAGATCCTTTG+AGG | 0.470260 | 4.1:+55073342 | MS.gene95714:CDS |
| CCTCAAAGGATCTTCCTAGT+AGG | 0.477106 | 4.1:-55073342 | None:intergenic |
| ATATCCTTGCCTCCTGCTGA+AGG | 0.499020 | 4.1:-55073058 | None:intergenic |
| CCTCCTGCTGAAGGTGAGAG+TGG | 0.549249 | 4.1:-55073049 | None:intergenic |
| GGATCTTCCTAGTAGGAAGA+GGG | 0.550819 | 4.1:-55073335 | None:intergenic |
| ACACCACTCTCACCTTCAGC+AGG | 0.551528 | 4.1:+55073046 | MS.gene95714:CDS |
| AGGATCTTCCTAGTAGGAAG+AGG | 0.558203 | 4.1:-55073336 | None:intergenic |
| CTCACCTTCAGCAGGAGGCA+AGG | 0.577485 | 4.1:+55073054 | MS.gene95714:CDS |
| ATGAGCTTCTTGGTTTACAA+GGG | 0.601511 | 4.1:+55073079 | MS.gene95714:CDS |
| TAAAAGACCTAAGATAAAGA+GGG | 0.605297 | 4.1:+55073212 | MS.gene95714:CDS |
| GACGCAGAAGAGCCAAACAA+AGG | 0.632309 | 4.1:+55073133 | MS.gene95714:CDS |
| GATCTTCCTAGTAGGAAGAG+GGG | 0.633617 | 4.1:-55073334 | None:intergenic |
| CCACTCTCACCTTCAGCAGG+AGG | 0.635931 | 4.1:+55073049 | MS.gene95714:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAAGACCTAAGATAAAGA+GGG | + | chr4.1:55073212-55073231 | MS.gene95714:CDS | 25.0% |
| CTAAAAGACCTAAGATAAAG+AGG | + | chr4.1:55073211-55073230 | MS.gene95714:CDS | 30.0% | |
| ! | CTCTTTATCTTAGGTCTTTT+AGG | - | chr4.1:55073213-55073232 | None:intergenic | 30.0% |
| ! | GAGAAAGATTTTCCTTTGTT+TGG | - | chr4.1:55073148-55073167 | None:intergenic | 30.0% |
| !!! | GTTTTGTTGTTTATGAGAAC+TGG | + | chr4.1:55073267-55073286 | MS.gene95714:CDS | 30.0% |
| ATGAGCTTCTTGGTTTACAA+GGG | + | chr4.1:55073079-55073098 | MS.gene95714:CDS | 35.0% | |
| TATGAGCTTCTTGGTTTACA+AGG | + | chr4.1:55073078-55073097 | MS.gene95714:CDS | 35.0% | |
| ACAGCAACCCTCTTTATCTT+AGG | - | chr4.1:55073222-55073241 | None:intergenic | 40.0% | |
| ! | GATAAAGAGGGTTGCTGTTA+AGG | + | chr4.1:55073224-55073243 | MS.gene95714:CDS | 40.0% |
| AGGATCTTCCTAGTAGGAAG+AGG | - | chr4.1:55073339-55073358 | None:intergenic | 45.0% | |
| AGGCAAGGATATGAGCTTCT+TGG | + | chr4.1:55073069-55073088 | MS.gene95714:CDS | 45.0% | |
| GATCTTCCTAGTAGGAAGAG+GGG | - | chr4.1:55073337-55073356 | None:intergenic | 45.0% | |
| GGATCTTCCTAGTAGGAAGA+GGG | - | chr4.1:55073338-55073357 | None:intergenic | 45.0% | |
| ATATCCTTGCCTCCTGCTGA+AGG | - | chr4.1:55073061-55073080 | None:intergenic | 50.0% | |
| GACGCAGAAGAGCCAAACAA+AGG | + | chr4.1:55073133-55073152 | MS.gene95714:CDS | 50.0% | |
| ACACCACTCTCACCTTCAGC+AGG | + | chr4.1:55073046-55073065 | MS.gene95714:CDS | 55.0% | |
| CTAGCTCCCCTCTTCCTACT+AGG | + | chr4.1:55073328-55073347 | MS.gene95714:CDS | 55.0% | |
| CCACTCTCACCTTCAGCAGG+AGG | + | chr4.1:55073049-55073068 | MS.gene95714:CDS | 60.0% | |
| CCTCCTGCTGAAGGTGAGAG+TGG | - | chr4.1:55073052-55073071 | None:intergenic | 60.0% | |
| CTCACCTTCAGCAGGAGGCA+AGG | + | chr4.1:55073054-55073073 | MS.gene95714:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 55073027 | 55073362 | 55073027 | ID=MS.gene95714 |
| chr4.1 | mRNA | 55073027 | 55073362 | 55073027 | ID=MS.gene95714.t1;Parent=MS.gene95714 |
| chr4.1 | exon | 55073027 | 55073362 | 55073027 | ID=MS.gene95714.t1.exon1;Parent=MS.gene95714.t1 |
| chr4.1 | CDS | 55073027 | 55073362 | 55073027 | ID=cds.MS.gene95714.t1;Parent=MS.gene95714.t1 |
| Gene Sequence |
| Protein sequence |
