Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96146.t1 | XP_003609563.1 | 68.2 | 66 | 17 | 2 | 18 | 83 | 374 | 435 | 9.20E-10 | 72.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96146.t1 | Q8LBI9 | 84.2 | 38 | 6 | 0 | 20 | 57 | 368 | 405 | 1.1e-08 | 60.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96146.t1 | G7JJ10 | 68.2 | 66 | 17 | 2 | 18 | 83 | 374 | 435 | 6.7e-10 | 72.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049272 | MS.gene96146 | 0.874491 | 6.48E-68 | -1.69E-46 |
| MS.gene050156 | MS.gene96146 | 0.823988 | 1.03E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene96146 | 0.800584 | 1.35E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene96146 | 0.824204 | 9.16E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene96146 | 0.812958 | 3.26E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene96146 | 0.810644 | 1.04E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene96146 | 0.814841 | 1.25E-51 | -1.69E-46 |
| MS.gene052042 | MS.gene96146 | 0.803062 | 4.17E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene96146 | 0.822418 | 2.39E-53 | -1.69E-46 |
| MS.gene053221 | MS.gene96146 | 0.800738 | 1.25E-48 | -1.69E-46 |
| MS.gene054139 | MS.gene96146 | 0.804313 | 2.29E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene96146 | 0.840639 | 7.70E-58 | -1.69E-46 |
| MS.gene055818 | MS.gene96146 | 0.823752 | 1.17E-53 | -1.69E-46 |
| MS.gene056506 | MS.gene96146 | 0.804204 | 2.42E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene96146 | 0.84887 | 4.65E-60 | -1.69E-46 |
| MS.gene058037 | MS.gene96146 | 0.804189 | 2.43E-49 | -1.69E-46 |
| MS.gene058829 | MS.gene96146 | 0.823864 | 1.10E-53 | -1.69E-46 |
| MS.gene059570 | MS.gene96146 | 0.803789 | 2.95E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene96146 | 0.810832 | 9.47E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene96146 | 0.833084 | 6.53E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene96146 | 0.814899 | 1.22E-51 | -1.69E-46 |
| MS.gene060781 | MS.gene96146 | 0.802476 | 5.51E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96146.t1 | MTR_4g118610 | 68.182 | 66 | 17 | 2 | 18 | 83 | 374 | 435 | 1.23e-18 | 79.0 |
| MS.gene96146.t1 | MTR_4g118610 | 90.000 | 40 | 4 | 0 | 18 | 57 | 374 | 413 | 4.05e-18 | 77.4 |
| MS.gene96146.t1 | MTR_2g020710 | 51.515 | 66 | 28 | 2 | 18 | 83 | 371 | 432 | 3.04e-12 | 60.8 |
| MS.gene96146.t1 | MTR_2g020710 | 57.447 | 47 | 20 | 0 | 18 | 64 | 371 | 417 | 5.42e-12 | 60.1 |
| MS.gene96146.t1 | MTR_2g020710 | 51.515 | 66 | 28 | 2 | 18 | 83 | 282 | 343 | 7.53e-12 | 59.7 |
| MS.gene96146.t1 | MTR_2g020710 | 66.667 | 39 | 13 | 0 | 18 | 56 | 371 | 409 | 1.41e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96146.t1 | AT5G18840 | 82.051 | 39 | 7 | 0 | 20 | 58 | 368 | 406 | 2.92e-14 | 66.6 |
Find 20 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAGCTGTTGTCGGCATTC+TGG | 0.328804 | 1.4:+21263210 | MS.gene96146:CDS |
| TTGGAATGGGACCGGTTCCT+TGG | 0.379291 | 1.4:+21263364 | MS.gene96146:CDS |
| CGCACGGACACCGACAAACT+TGG | 0.409012 | 1.4:-21262310 | None:intergenic |
| ATGCCGACAACAGCTAATAT+TGG | 0.437641 | 1.4:-21263205 | None:intergenic |
| GTTGGCAGTACTGCTGCTGC+TGG | 0.442298 | 1.4:+21263560 | MS.gene96146:CDS |
| TGGAATGGGACCGGTTCCTT+GGG | 0.453222 | 1.4:+21263365 | MS.gene96146:CDS |
| ATCCAAGTCACACACAATCT+TGG | 0.455959 | 1.4:+21263496 | MS.gene96146:CDS |
| TACATTATTACCCAAGGAAC+CGG | 0.469917 | 1.4:-21263375 | None:intergenic |
| CACCAAGATTGTGTGTGACT+TGG | 0.490961 | 1.4:-21263498 | None:intergenic |
| CGACAAACTTGGAGCGTTGG+TGG | 0.492822 | 1.4:-21262299 | None:intergenic |
| TCCTTGGGTAATAATGTATG+AGG | 0.499064 | 1.4:+21263380 | MS.gene96146:CDS |
| AGCGTTGGTGGTTCCGTTGG+TGG | 0.514223 | 1.4:-21262287 | None:intergenic |
| TCAGAACTTGCTGCTTGAGT+TGG | 0.516261 | 1.4:+21263180 | MS.gene96146:CDS |
| CACCAACGCTCCAAGTTTGT+CGG | 0.519106 | 1.4:+21262300 | MS.gene96146:CDS |
| GTACCAATATTAGCTGTTGT+CGG | 0.525827 | 1.4:+21263202 | MS.gene96146:CDS |
| CACCGACAAACTTGGAGCGT+TGG | 0.534988 | 1.4:-21262302 | None:intergenic |
| TGGAGCGTTGGTGGTTCCGT+TGG | 0.543892 | 1.4:-21262290 | None:intergenic |
| CAGTTTCATGGAACCACCAA+CGG | 0.584419 | 1.4:+21262274 | None:intergenic |
| CTATTGCAGCTTACTTCGCA+CGG | 0.591502 | 1.4:-21262326 | None:intergenic |
| ACCTCATACATTATTACCCA+AGG | 0.600251 | 1.4:-21263381 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTTGAAAATGTTAATCA+AGG | + | chr1.4:21263239-21263258 | MS.gene96146:intron | 15.0% |
| !!! | GTTGTTTAAATTTGTTTATT+TGG | + | chr1.4:21262655-21262674 | MS.gene96146:intron | 15.0% |
| !!! | TTTGATAATACCATTAATAA+GGG | + | chr1.4:21263016-21263035 | MS.gene96146:intron | 15.0% |
| !!! | TTTTGATAATACCATTAATA+AGG | + | chr1.4:21263015-21263034 | MS.gene96146:intron | 15.0% |
| !! | ATACAATTTGATACAATTTC+AGG | - | chr1.4:21263319-21263338 | None:intergenic | 20.0% |
| !! | ATATGCTTACGATAAAAATA+TGG | + | chr1.4:21262604-21262623 | MS.gene96146:intron | 20.0% |
| !! | ATTGTATGAGAAATTCATAT+AGG | - | chr1.4:21262842-21262861 | None:intergenic | 20.0% |
| !! | CATATATTAAATTGAATGAC+AGG | + | chr1.4:21262897-21262916 | MS.gene96146:intron | 20.0% |
| !! | TTGTATGAGAAATTCATATA+GGG | - | chr1.4:21262841-21262860 | None:intergenic | 20.0% |
| !!! | AATTTGTTTATTTGGCATTT+CGG | + | chr1.4:21262663-21262682 | MS.gene96146:intron | 20.0% |
| !!! | TGTTATGATTAATTTGATTG+AGG | + | chr1.4:21262470-21262489 | MS.gene96146:intron | 20.0% |
| !!! | TTGATAATACCATTAATAAG+GGG | + | chr1.4:21263017-21263036 | MS.gene96146:intron | 20.0% |
| ! | GAAAATGTTAATCAAGGAAT+TGG | + | chr1.4:21263245-21263264 | MS.gene96146:intron | 25.0% |
| ! | TGATAAATCCAACAACATAA+TGG | - | chr1.4:21262759-21262778 | None:intergenic | 25.0% |
| ! | TGCATATAAACACTTCTATT+AGG | - | chr1.4:21263048-21263067 | None:intergenic | 25.0% |
| ! | TTACCGTTATAACTTATAAG+CGG | - | chr1.4:21262973-21262992 | None:intergenic | 25.0% |
| ! | TTAGTTTAATGAGAATCCAA+AGG | - | chr1.4:21262371-21262390 | None:intergenic | 25.0% |
| ! | TTTGGATTCTCATTAAACTA+AGG | + | chr1.4:21262370-21262389 | MS.gene96146:intron | 25.0% |
| !! | AACTTTGCTATATTATTCCA+TGG | + | chr1.4:21263150-21263169 | MS.gene96146:intron | 25.0% |
| !! | AATTATGACTGATTCAGTTT+CGG | + | chr1.4:21262400-21262419 | MS.gene96146:intron | 25.0% |
| AGACCGCTTATAAGTTATAA+CGG | + | chr1.4:21262967-21262986 | MS.gene96146:intron | 30.0% | |
| CTTATTAACAATTGTGCTCA+TGG | + | chr1.4:21263458-21263477 | MS.gene96146:intron | 30.0% | |
| GAGATATAGTGTACATCATT+TGG | + | chr1.4:21262711-21262730 | MS.gene96146:intron | 30.0% | |
| TATCCAAAAAGTTGCAGATA+GGG | + | chr1.4:21262803-21262822 | MS.gene96146:intron | 30.0% | |
| TCCAAACTTATCCATCAAAA+TGG | - | chr1.4:21262944-21262963 | None:intergenic | 30.0% | |
| ! | AATCCAAAGGCAAAATTTTC+TGG | - | chr1.4:21262358-21262377 | None:intergenic | 30.0% |
| ! | ACAGGTTCCATTCATTATTT+TGG | + | chr1.4:21262915-21262934 | MS.gene96146:intron | 30.0% |
| ! | CAGGTTCCATTCATTATTTT+GGG | + | chr1.4:21262916-21262935 | MS.gene96146:intron | 30.0% |
| ! | TGATTAATTTGATTGAGGTG+TGG | + | chr1.4:21262475-21262494 | MS.gene96146:intron | 30.0% |
| !! | AAGCACACTTAATGGTTTTA+TGG | + | chr1.4:21263419-21263438 | MS.gene96146:intron | 30.0% |
| !! | AGAAGTGTTTATATGCATGT+AGG | + | chr1.4:21263051-21263070 | MS.gene96146:intron | 30.0% |
| AAAAAATAAGCAAGCTGCAC+AGG | - | chr1.4:21263526-21263545 | None:intergenic | 35.0% | |
| AAAAATAAGCAAGCTGCACA+GGG | - | chr1.4:21263525-21263544 | None:intergenic | 35.0% | |
| ACCTCATACATTATTACCCA+AGG | - | chr1.4:21263384-21263403 | None:intergenic | 35.0% | |
| ATCCAAAAAGTTGCAGATAG+GGG | + | chr1.4:21262804-21262823 | MS.gene96146:intron | 35.0% | |
| ATGGCTCCCAAAATAATGAA+TGG | - | chr1.4:21262925-21262944 | None:intergenic | 35.0% | |
| ATTTGGCAAAGCACACTTAA+TGG | + | chr1.4:21263411-21263430 | MS.gene96146:intron | 35.0% | |
| CTATCCAAAAAGTTGCAGAT+AGG | + | chr1.4:21262802-21262821 | MS.gene96146:intron | 35.0% | |
| GCTATATTATTCCATGGATC+AGG | + | chr1.4:21263156-21263175 | MS.gene96146:intron | 35.0% | |
| GTACCAATATTAGCTGTTGT+CGG | + | chr1.4:21263202-21263221 | MS.gene96146:CDS | 35.0% | |
| TACATTATTACCCAAGGAAC+CGG | - | chr1.4:21263378-21263397 | None:intergenic | 35.0% | |
| TAGGCATTTCCCCTTATTAA+TGG | - | chr1.4:21263029-21263048 | None:intergenic | 35.0% | |
| TCCTTGGGTAATAATGTATG+AGG | + | chr1.4:21263380-21263399 | MS.gene96146:CDS | 35.0% | |
| ! | ATTTCGGTTGAAGCATTTAG+TGG | + | chr1.4:21262679-21262698 | MS.gene96146:intron | 35.0% |
| ! | CATCCAGAAAATTTTGCCTT+TGG | + | chr1.4:21262352-21262371 | MS.gene96146:intron | 35.0% |
| ! | TGTATGAGGTATGTGTCATT+TGG | + | chr1.4:21263394-21263413 | MS.gene96146:intron | 35.0% |
| !! | GCCATTTTGATGGATAAGTT+TGG | + | chr1.4:21262940-21262959 | MS.gene96146:intron | 35.0% |
| !! | TATTTTGGGAGCCATTTTGA+TGG | + | chr1.4:21262930-21262949 | MS.gene96146:intron | 35.0% |
| !!! | CAGCTTGCTTATTTTTTGCT+TGG | + | chr1.4:21263529-21263548 | MS.gene96146:CDS | 35.0% |
| !!! | GCTTATTTTTTGCTTGGTAG+AGG | + | chr1.4:21263535-21263554 | MS.gene96146:CDS | 35.0% |
| ATCCAAGTCACACACAATCT+TGG | + | chr1.4:21263496-21263515 | MS.gene96146:CDS | 40.0% | |
| ATGCCGACAACAGCTAATAT+TGG | - | chr1.4:21263208-21263227 | None:intergenic | 40.0% | |
| GGTTTGAGGGAAATTCGTTA+TGG | + | chr1.4:21262515-21262534 | MS.gene96146:intron | 40.0% | |
| GTGTGTTGCCATTATGTTGT+TGG | + | chr1.4:21262748-21262767 | MS.gene96146:intron | 40.0% | |
| TGGATTTATCACCAAGAAGC+TGG | + | chr1.4:21262768-21262787 | MS.gene96146:intron | 40.0% | |
| TTATCACCAAGAAGCTGGAA+GGG | + | chr1.4:21262773-21262792 | MS.gene96146:intron | 40.0% | |
| TTTATCACCAAGAAGCTGGA+AGG | + | chr1.4:21262772-21262791 | MS.gene96146:intron | 40.0% | |
| ! | AGCAGCATTTTCGATTGGAA+TGG | + | chr1.4:21263350-21263369 | MS.gene96146:CDS | 40.0% |
| ! | ATTTTCGATTGGAATGGGAC+CGG | + | chr1.4:21263356-21263375 | MS.gene96146:CDS | 40.0% |
| ! | GCAGCATTTTCGATTGGAAT+GGG | + | chr1.4:21263351-21263370 | MS.gene96146:CDS | 40.0% |
| ! | TACCCCTATCTGCAACTTTT+TGG | - | chr1.4:21262809-21262828 | None:intergenic | 40.0% |
| ! | TACGTAGCAGCATTTTCGAT+TGG | + | chr1.4:21263345-21263364 | MS.gene96146:CDS | 40.0% |
| ! | TTTTGCTTGGTAGAGGTTGT+TGG | + | chr1.4:21263542-21263561 | MS.gene96146:CDS | 40.0% |
| !! | GAGATTGCTTGATTTGCAGA+TGG | + | chr1.4:21262542-21262561 | MS.gene96146:intron | 40.0% |
| ATTAGCTGTTGTCGGCATTC+TGG | + | chr1.4:21263210-21263229 | MS.gene96146:CDS | 45.0% | |
| ATTGTGTGTGTGACGGTTTG+AGG | + | chr1.4:21262501-21262520 | MS.gene96146:intron | 45.0% | |
| CACCAAGATTGTGTGTGACT+TGG | - | chr1.4:21263501-21263520 | None:intergenic | 45.0% | |
| CTATTGCAGCTTACTTCGCA+CGG | - | chr1.4:21262329-21262348 | None:intergenic | 45.0% | |
| GTGGTGTATTGTGTGTGTGA+CGG | + | chr1.4:21262494-21262513 | MS.gene96146:intron | 45.0% | |
| TGCATGTAGGCTTCAACAAG+TGG | + | chr1.4:21263064-21263083 | MS.gene96146:intron | 45.0% | |
| TTGTGTGTGTGACGGTTTGA+GGG | + | chr1.4:21262502-21262521 | MS.gene96146:intron | 45.0% | |
| ! | CAAGTTCTGATCCTGATCCA+TGG | - | chr1.4:21263170-21263189 | None:intergenic | 45.0% |
| !! | TCAGAACTTGCTGCTTGAGT+TGG | + | chr1.4:21263180-21263199 | MS.gene96146:CDS | 45.0% |
| AACCAACCCTTCCAGCTTCT+TGG | - | chr1.4:21262782-21262801 | None:intergenic | 50.0% | |
| CACCAACGCTCCAAGTTTGT+CGG | + | chr1.4:21262300-21262319 | MS.gene96146:CDS | 50.0% | |
| GAGGGAAATTCGTTATGGCG+AGG | + | chr1.4:21262520-21262539 | MS.gene96146:intron | 50.0% | |
| CACCAAGAAGCTGGAAGGGT+TGG | + | chr1.4:21262777-21262796 | MS.gene96146:intron | 55.0% | |
| CACCGACAAACTTGGAGCGT+TGG | - | chr1.4:21262305-21262324 | None:intergenic | 55.0% | |
| ! | TGGAATGGGACCGGTTCCTT+GGG | + | chr1.4:21263365-21263384 | MS.gene96146:CDS | 55.0% |
| ! | TTGGAATGGGACCGGTTCCT+TGG | + | chr1.4:21263364-21263383 | MS.gene96146:CDS | 55.0% |
| !! | CGACAAACTTGGAGCGTTGG+TGG | - | chr1.4:21262302-21262321 | None:intergenic | 55.0% |
| CGCACGGACACCGACAAACT+TGG | - | chr1.4:21262313-21262332 | None:intergenic | 60.0% | |
| GTTGGCAGTACTGCTGCTGC+TGG | + | chr1.4:21263560-21263579 | MS.gene96146:CDS | 60.0% | |
| !! | AGCGTTGGTGGTTCCGTTGG+TGG | - | chr1.4:21262290-21262309 | None:intergenic | 60.0% |
| !! | TGGAGCGTTGGTGGTTCCGT+TGG | - | chr1.4:21262293-21262312 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 21262281 | 21263588 | 21262281 | ID=MS.gene96146 |
| chr1.4 | mRNA | 21262281 | 21263588 | 21262281 | ID=MS.gene96146.t1;Parent=MS.gene96146 |
| chr1.4 | exon | 21262281 | 21262334 | 21262281 | ID=MS.gene96146.t1.exon1;Parent=MS.gene96146.t1 |
| chr1.4 | CDS | 21262281 | 21262334 | 21262281 | ID=cds.MS.gene96146.t1;Parent=MS.gene96146.t1 |
| chr1.4 | exon | 21263178 | 21263231 | 21263178 | ID=MS.gene96146.t1.exon2;Parent=MS.gene96146.t1 |
| chr1.4 | CDS | 21263178 | 21263231 | 21263178 | ID=cds.MS.gene96146.t1;Parent=MS.gene96146.t1 |
| chr1.4 | exon | 21263342 | 21263401 | 21263342 | ID=MS.gene96146.t1.exon3;Parent=MS.gene96146.t1 |
| chr1.4 | CDS | 21263342 | 21263401 | 21263342 | ID=cds.MS.gene96146.t1;Parent=MS.gene96146.t1 |
| chr1.4 | exon | 21263484 | 21263588 | 21263484 | ID=MS.gene96146.t1.exon4;Parent=MS.gene96146.t1 |
| chr1.4 | CDS | 21263484 | 21263588 | 21263484 | ID=cds.MS.gene96146.t1;Parent=MS.gene96146.t1 |
| Gene Sequence |
| Protein sequence |
