AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049272


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049272.t1 MTR_4g118610 69.231 65 16 2 59 123 375 435 1.81e-17 77.4
MS.gene049272.t1 MTR_4g118610 92.308 39 3 0 59 97 375 413 5.71e-17 75.9
MS.gene049272.t1 MTR_2g020710 52.308 65 27 2 59 123 372 432 2.38e-11 60.1
MS.gene049272.t1 MTR_2g020710 58.696 46 19 0 59 104 372 417 4.56e-11 59.3
MS.gene049272.t1 MTR_2g020710 52.308 65 27 2 59 123 283 343 6.66e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049272.t1 AT5G18840 82.051 39 7 0 60 98 368 406 1.91e-13 66.2

Find 21 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAACCCTTCCAGCTTCT+TGG 0.243031 3.3:-79884252 None:intergenic
ATTAGCTGTTGTCGGCATTC+TGG 0.328804 3.3:+79884683 MS.gene049272:CDS
TTGGAATGGGACCGGTTCCT+TGG 0.379291 3.3:+79884837 MS.gene049272:CDS
GTGTGTTGCCATTATGTTGT+TGG 0.386442 3.3:+79884221 MS.gene049272:CDS
TGATAAATCCAACAACATAA+TGG 0.420838 3.3:-79884229 None:intergenic
ATGCCGACAACAGCTAATAT+TGG 0.437641 3.3:-79884678 None:intergenic
GTTGGCAGTACTGCTGCTGC+TGG 0.442298 3.3:+79885033 MS.gene049272:CDS
TGGAATGGGACCGGTTCCTT+GGG 0.453222 3.3:+79884838 MS.gene049272:CDS
ATCCAAGTCACACACAATCT+TGG 0.455959 3.3:+79884969 MS.gene049272:CDS
TACATTATTACCCAAGGAAC+CGG 0.469917 3.3:-79884848 None:intergenic
TGGATTTATCACCAAGAAGC+TGG 0.490960 3.3:+79884241 MS.gene049272:CDS
CACCAAGATTGTGTGTGACT+TGG 0.490961 3.3:-79884971 None:intergenic
CACCAAGAAGCTGGAAGGGT+TGG 0.493905 3.3:+79884250 MS.gene049272:CDS
TCCTTGGGTAATAATGTATG+AGG 0.499064 3.3:+79884853 MS.gene049272:CDS
TCAGAACTTGCTGCTTGAGT+TGG 0.516261 3.3:+79884653 MS.gene049272:CDS
GTACCAATATTAGCTGTTGT+CGG 0.525827 3.3:+79884675 MS.gene049272:CDS
GAGATTGCTTGATTTGCAGA+TGG 0.540081 3.3:+79884015 MS.gene049272:CDS
TTTATCACCAAGAAGCTGGA+AGG 0.559494 3.3:+79884245 MS.gene049272:CDS
ACCTCATACATTATTACCCA+AGG 0.600251 3.3:-79884854 None:intergenic
TTATCACCAAGAAGCTGGAA+GGG 0.610575 3.3:+79884246 MS.gene049272:CDS
GAGGGAAATTCGTTATGGCG+AGG 0.635294 3.3:+79883993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTGAAAATGTTAATCA+AGG + chr3.3:79884712-79884731 MS.gene049272:intron 15.0%
!!! GTTGTTTAAATTTGTTTATT+TGG + chr3.3:79884128-79884147 MS.gene049272:intron 15.0%
!!! TTTGATAATACCATTAATAA+GGG + chr3.3:79884489-79884508 MS.gene049272:intron 15.0%
!!! TTTTGATAATACCATTAATA+AGG + chr3.3:79884488-79884507 MS.gene049272:intron 15.0%
!! ATACAATTTGATACAATTTC+AGG - chr3.3:79884792-79884811 None:intergenic 20.0%
!! ATATGCTTACGATAAAAATA+TGG + chr3.3:79884077-79884096 MS.gene049272:CDS 20.0%
!! ATTGTATGAGAAATTCATAT+AGG - chr3.3:79884315-79884334 None:intergenic 20.0%
!! CATATATTAAATTGAATGAC+AGG + chr3.3:79884370-79884389 MS.gene049272:intron 20.0%
!! TTGTATGAGAAATTCATATA+GGG - chr3.3:79884314-79884333 None:intergenic 20.0%
!!! AATTTGTTTATTTGGCATTT+CGG + chr3.3:79884136-79884155 MS.gene049272:intron 20.0%
!!! TTGATAATACCATTAATAAG+GGG + chr3.3:79884490-79884509 MS.gene049272:intron 20.0%
! GAAAATGTTAATCAAGGAAT+TGG + chr3.3:79884718-79884737 MS.gene049272:intron 25.0%
! TGATAAATCCAACAACATAA+TGG - chr3.3:79884232-79884251 None:intergenic 25.0%
! TGCATATAAACACTTCTATT+AGG - chr3.3:79884521-79884540 None:intergenic 25.0%
! TTACCGTTATAACTTATAAG+CGG - chr3.3:79884446-79884465 None:intergenic 25.0%
!! AACTTTGCTATATTATTCCA+TGG + chr3.3:79884623-79884642 MS.gene049272:intron 25.0%
AGACCGCTTATAAGTTATAA+CGG + chr3.3:79884440-79884459 MS.gene049272:intron 30.0%
CTTATTAACAATTGTGCTCA+TGG + chr3.3:79884931-79884950 MS.gene049272:intron 30.0%
GAGATATAGTGTACATCATT+TGG + chr3.3:79884184-79884203 MS.gene049272:intron 30.0%
TATGCAAAAAGTTGCAGATA+GGG + chr3.3:79884276-79884295 MS.gene049272:CDS 30.0%
TCCAAACTTATCCATCAAAA+TGG - chr3.3:79884417-79884436 None:intergenic 30.0%
! ACAGGTTCCATTCATTATTT+TGG + chr3.3:79884388-79884407 MS.gene049272:intron 30.0%
! CAGGTTCCATTCATTATTTT+GGG + chr3.3:79884389-79884408 MS.gene049272:intron 30.0%
!! AAGCACACTTAATGGTTTTA+TGG + chr3.3:79884892-79884911 MS.gene049272:intron 30.0%
!! AGAAGTGTTTATATGCATGT+AGG + chr3.3:79884524-79884543 MS.gene049272:intron 30.0%
AAAAAATAAGCAAGCTGCAC+AGG - chr3.3:79884999-79885018 None:intergenic 35.0%
AAAAATAAGCAAGCTGCACA+GGG - chr3.3:79884998-79885017 None:intergenic 35.0%
ACCTCATACATTATTACCCA+AGG - chr3.3:79884857-79884876 None:intergenic 35.0%
ATGCAAAAAGTTGCAGATAG+GGG + chr3.3:79884277-79884296 MS.gene049272:CDS 35.0%
ATGGCTCCCAAAATAATGAA+TGG - chr3.3:79884398-79884417 None:intergenic 35.0%
ATTTGGCAAAGCACACTTAA+TGG + chr3.3:79884884-79884903 MS.gene049272:intron 35.0%
CTATGCAAAAAGTTGCAGAT+AGG + chr3.3:79884275-79884294 MS.gene049272:CDS 35.0%
GCTATATTATTCCATGGATC+AGG + chr3.3:79884629-79884648 MS.gene049272:intron 35.0%
GTACCAATATTAGCTGTTGT+CGG + chr3.3:79884675-79884694 MS.gene049272:CDS 35.0%
TACATTATTACCCAAGGAAC+CGG - chr3.3:79884851-79884870 None:intergenic 35.0%
TAGGCATTTCCCCTTATTAA+TGG - chr3.3:79884502-79884521 None:intergenic 35.0%
TCCTTGGGTAATAATGTATG+AGG + chr3.3:79884853-79884872 MS.gene049272:CDS 35.0%
! ATTTCGGTTGAAGCATTTAG+TGG + chr3.3:79884152-79884171 MS.gene049272:intron 35.0%
! TGTATGAGGTATGTGTCATT+TGG + chr3.3:79884867-79884886 MS.gene049272:intron 35.0%
!! GCCATTTTGATGGATAAGTT+TGG + chr3.3:79884413-79884432 MS.gene049272:intron 35.0%
!! TATTTTGGGAGCCATTTTGA+TGG + chr3.3:79884403-79884422 MS.gene049272:intron 35.0%
!!! CAGCTTGCTTATTTTTTGCT+TGG + chr3.3:79885002-79885021 MS.gene049272:CDS 35.0%
!!! GCTTATTTTTTGCTTGGTAG+AGG + chr3.3:79885008-79885027 MS.gene049272:CDS 35.0%
ATCCAAGTCACACACAATCT+TGG + chr3.3:79884969-79884988 MS.gene049272:CDS 40.0%
ATGCCGACAACAGCTAATAT+TGG - chr3.3:79884681-79884700 None:intergenic 40.0%
GTGTGTTGCCATTATGTTGT+TGG + chr3.3:79884221-79884240 MS.gene049272:CDS 40.0%
TGGATTTATCACCAAGAAGC+TGG + chr3.3:79884241-79884260 MS.gene049272:CDS 40.0%
TTATCACCAAGAAGCTGGAA+GGG + chr3.3:79884246-79884265 MS.gene049272:CDS 40.0%
TTTATCACCAAGAAGCTGGA+AGG + chr3.3:79884245-79884264 MS.gene049272:CDS 40.0%
! AGCAGCATTTTCGATTGGAA+TGG + chr3.3:79884823-79884842 MS.gene049272:CDS 40.0%
! ATTTTCGATTGGAATGGGAC+CGG + chr3.3:79884829-79884848 MS.gene049272:CDS 40.0%
! GCAGCATTTTCGATTGGAAT+GGG + chr3.3:79884824-79884843 MS.gene049272:CDS 40.0%
! TACGTAGCAGCATTTTCGAT+TGG + chr3.3:79884818-79884837 MS.gene049272:CDS 40.0%
! TTTTGCTTGGTAGAGGTTGT+TGG + chr3.3:79885015-79885034 MS.gene049272:CDS 40.0%
!! GAGATTGCTTGATTTGCAGA+TGG + chr3.3:79884015-79884034 MS.gene049272:CDS 40.0%
ATTAGCTGTTGTCGGCATTC+TGG + chr3.3:79884683-79884702 MS.gene049272:CDS 45.0%
CACCAAGATTGTGTGTGACT+TGG - chr3.3:79884974-79884993 None:intergenic 45.0%
TGCATGTAGGCTTCAACAAG+TGG + chr3.3:79884537-79884556 MS.gene049272:intron 45.0%
! CAAGTTCTGATCCTGATCCA+TGG - chr3.3:79884643-79884662 None:intergenic 45.0%
!! TCAGAACTTGCTGCTTGAGT+TGG + chr3.3:79884653-79884672 MS.gene049272:CDS 45.0%
AACCAACCCTTCCAGCTTCT+TGG - chr3.3:79884255-79884274 None:intergenic 50.0%
CACCAAGAAGCTGGAAGGGT+TGG + chr3.3:79884250-79884269 MS.gene049272:CDS 55.0%
! TGGAATGGGACCGGTTCCTT+GGG + chr3.3:79884838-79884857 MS.gene049272:CDS 55.0%
! TTGGAATGGGACCGGTTCCT+TGG + chr3.3:79884837-79884856 MS.gene049272:CDS 55.0%
GTTGGCAGTACTGCTGCTGC+TGG + chr3.3:79885033-79885052 MS.gene049272:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 79884007 79885061 79884007 ID=MS.gene049272
chr3.3 mRNA 79884007 79885061 79884007 ID=MS.gene049272.t1;Parent=MS.gene049272
chr3.3 exon 79884007 79884098 79884007 ID=MS.gene049272.t1.exon1;Parent=MS.gene049272.t1
chr3.3 CDS 79884007 79884098 79884007 ID=cds.MS.gene049272.t1;Parent=MS.gene049272.t1
chr3.3 exon 79884217 79884298 79884217 ID=MS.gene049272.t1.exon2;Parent=MS.gene049272.t1
chr3.3 CDS 79884217 79884298 79884217 ID=cds.MS.gene049272.t1;Parent=MS.gene049272.t1
chr3.3 exon 79884651 79884704 79884651 ID=MS.gene049272.t1.exon3;Parent=MS.gene049272.t1
chr3.3 CDS 79884651 79884704 79884651 ID=cds.MS.gene049272.t1;Parent=MS.gene049272.t1
chr3.3 exon 79884815 79884874 79884815 ID=MS.gene049272.t1.exon4;Parent=MS.gene049272.t1
chr3.3 CDS 79884815 79884874 79884815 ID=cds.MS.gene049272.t1;Parent=MS.gene049272.t1
chr3.3 exon 79884957 79885061 79884957 ID=MS.gene049272.t1.exon5;Parent=MS.gene049272.t1
chr3.3 CDS 79884957 79885061 79884957 ID=cds.MS.gene049272.t1;Parent=MS.gene049272.t1
Gene Sequence

>MS.gene049272

ATGGCGAGGAGATTGCTTGATTTGCAGATGGATATATTGTTGTGTGAAATGAGTATAATTCGTCTGCATCATATGCTTACGATAAAAATATGGTAAACTCATATTTGTTAATATGTTTTTTGTTGTTTAAATTTGTTTATTTGGCATTTCGGTTGAAGCATTTAGTGGTTTTGTTTTGAGATATAGTGTACATCATTTGGTAATTTTGAGTTTTGTGTGTTGCCATTATGTTGTTGGATTTATCACCAAGAAGCTGGAAGGGTTGGTTCTATGCAAAAAGTTGCAGATAGGGGTAGTTTACTATCCCTATATGAATTTCTCATACAATTATTTATTGATAATATAATTGTACTTTTATATCAGCATATATTAAATTGAATGACAGGTTCCATTCATTATTTTGGGAGCCATTTTGATGGATAAGTTTGGAAGAAGACCGCTTATAAGTTATAACGGTAATTCTGTTTTCAAGTATTTAAAATTTTGATAATACCATTAATAAGGGGAAATGCCTAATAGAAGTGTTTATATGCATGTAGGCTTCAACAAGTGGAACATTTTTAATCATATTTTCTCTTTAGATTATGATGTGTATCTCTCAATTAGTCAATTTTGAAACTTTGCTATATTATTCCATGGATCAGGATCAGAACTTGCTGCTTGAGTTGGTACCAATATTAGCTGTTGTCGGCATTCTGGTGAGTCTAATTTGAAAATGTTAATCAAGGAATTGGAGTTAATGAAAAGTTAGTTAATCAGATACATTCTTTAAGCATATTTGACCTGAAATTGTATCAAATTGTATCAGATATACGTAGCAGCATTTTCGATTGGAATGGGACCGGTTCCTTGGGTAATAATGTATGAGGTATGTGTCATTTGGCAAAGCACACTTAATGGTTTTATGGCTTTCGTAGTGCAACTCTTATTAACAATTGTGCTCATGGAAGATATTGAGAAAAATCCAAGTCACACACAATCTTGGTGTCCCTGTGCAGCTTGCTTATTTTTTGCTTGGTAGAGGTTGTTGGCAGTACTGCTGCTGCTGGTTTTGA

Protein sequence

>MS.gene049272.t1

MARRLLDLQMDILLCEMSIIRLHHMLTIKICFVCCHYVVGFITKKLEGLVLCKKLQIGDQNLLLELVPILAVVGILIYVAAFSIGMGPVPWVIMYEILRKIQVTHNLGVPVQLAYFLLGRGCWQYCCCWF