Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98927.t1 | XP_003629262.1 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 8.80E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98927.t1 | Q8L7E9 | 80.8 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 8.1e-53 | 207.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98927.t1 | G7LB65 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 6.4e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene98927 | 0.852591 | 4.17E-61 | -1.69E-46 |
| MS.gene049272 | MS.gene98927 | 0.803911 | 2.78E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene98927 | 0.822612 | 2.16E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene98927 | 0.804719 | 1.89E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene98927 | 0.82014 | 8.03E-53 | -1.69E-46 |
| MS.gene050156 | MS.gene98927 | 0.822627 | 2.14E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene98927 | 0.807587 | 4.70E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene98927 | 0.820915 | 5.33E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene98927 | 0.835198 | 1.93E-56 | -1.69E-46 |
| MS.gene052582 | MS.gene98927 | 0.824832 | 6.52E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene98927 | 0.829272 | 5.65E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene98927 | 0.827854 | 1.24E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene98927 | 0.805771 | 1.14E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene98927 | 0.804685 | 1.92E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene98927 | 0.817039 | 4.05E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene98927 | 0.808563 | 2.91E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene98927 | 0.807734 | 4.37E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene98927 | 0.822954 | 1.80E-53 | -1.69E-46 |
| MS.gene054775 | MS.gene98927 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene055166 | MS.gene98927 | 0.808268 | 3.37E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene98927 | 0.821119 | 4.78E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene98927 | 0.809918 | 1.49E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene98927 | 0.802902 | 4.50E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene98927 | 0.85287 | 3.48E-61 | -1.69E-46 |
| MS.gene058037 | MS.gene98927 | 0.816715 | 4.79E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene98927 | 0.812417 | 4.28E-51 | -1.69E-46 |
| MS.gene059570 | MS.gene98927 | 0.805645 | 1.21E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene98927 | 0.817481 | 3.23E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene98927 | 0.824268 | 8.85E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98927.t1 | MTR_8g075150 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 6.70e-82 | 246 |
| MS.gene98927.t1 | MTR_5g022670 | 82.114 | 123 | 22 | 0 | 3 | 125 | 2 | 124 | 8.18e-72 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98927.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 2.22e-70 | 217 |
| MS.gene98927.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 2.22e-70 | 217 |
| MS.gene98927.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 2.22e-70 | 217 |
| MS.gene98927.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 2.22e-70 | 217 |
| MS.gene98927.t1 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 2.22e-70 | 217 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.38e-59 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-58 | 186 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.23e-58 | 186 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.23e-58 | 186 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.61e-58 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.91e-58 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.91e-58 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.91e-58 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.91e-58 | 185 |
| MS.gene98927.t1 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 2.91e-58 | 185 |
Find 24 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGAGGCTCTCGGGTTTC+AGG | 0.140533 | 6.2:+38206226 | None:intergenic |
| CTGCATCTAAGCCTATTAAT+TGG | 0.182088 | 6.2:+38206386 | None:intergenic |
| TTTGAGAAGGTCTTCTATTT+TGG | 0.212238 | 6.2:-38206188 | MS.gene98927:CDS |
| GGAACGTTGTTTATCAATTT+AGG | 0.249469 | 6.2:-38206117 | MS.gene98927:CDS |
| GCAAATGTAGCCCAATTAAT+AGG | 0.272486 | 6.2:-38206397 | MS.gene98927:CDS |
| GCATCTTCATGGGAACAATT+TGG | 0.278027 | 6.2:-38206427 | MS.gene98927:CDS |
| GCACAACATCTTAAGTTGAT+TGG | 0.319124 | 6.2:-38206292 | MS.gene98927:CDS |
| TAGATGCAGTAAAGTTGATT+AGG | 0.362979 | 6.2:-38206372 | MS.gene98927:CDS |
| CACAACATCTTAAGTTGATT+GGG | 0.378407 | 6.2:-38206291 | MS.gene98927:CDS |
| CTAGTTGTTCTAGAGGCTCT+CGG | 0.379192 | 6.2:+38206218 | None:intergenic |
| GATATAGAATGGCATCTTCA+TGG | 0.416346 | 6.2:-38206438 | None:intergenic |
| TGCATCTAAGCCTATTAATT+GGG | 0.419039 | 6.2:+38206387 | None:intergenic |
| CTAGAGGCTCTCGGGTTTCA+GGG | 0.434211 | 6.2:+38206227 | None:intergenic |
| CTATCTTTATTTGCTCGCCA+TGG | 0.444150 | 6.2:-38206143 | MS.gene98927:CDS |
| TATCTTTATTTGCTCGCCAT+GGG | 0.487896 | 6.2:-38206142 | MS.gene98927:CDS |
| TAGTTGTTCTAGAGGCTCTC+GGG | 0.496041 | 6.2:+38206219 | None:intergenic |
| CATGGCGAGCAAATAAAGAT+AGG | 0.497179 | 6.2:+38206144 | None:intergenic |
| TTTATTTGCTCGCCATGGGA+TGG | 0.519477 | 6.2:-38206138 | MS.gene98927:CDS |
| ATATAGAATGGCATCTTCAT+GGG | 0.542232 | 6.2:-38206437 | None:intergenic |
| GCATCTTCTAGTTGTTCTAG+AGG | 0.556880 | 6.2:+38206211 | None:intergenic |
| CGTTGTTTATCAATTTAGGA+AGG | 0.558364 | 6.2:-38206113 | MS.gene98927:CDS |
| ATAAACAACGTTCCATCCCA+TGG | 0.601527 | 6.2:+38206126 | None:intergenic |
| AACTAGAAGATGCTTTGAGA+AGG | 0.613004 | 6.2:-38206201 | MS.gene98927:CDS |
| CGAAAGCAGCAAATACAGCA+AGG | 0.638455 | 6.2:-38206342 | MS.gene98927:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTTGTTTATCAATTTAGGA+AGG | - | chr6.2:38206389-38206408 | MS.gene98927:CDS | 30.0% | |
| GGAACGTTGTTTATCAATTT+AGG | - | chr6.2:38206385-38206404 | MS.gene98927:CDS | 30.0% | |
| TGCATCTAAGCCTATTAATT+GGG | + | chr6.2:38206118-38206137 | None:intergenic | 30.0% | |
| ! | TAGATGCAGTAAAGTTGATT+AGG | - | chr6.2:38206130-38206149 | MS.gene98927:CDS | 30.0% |
| ! | TTTGAGAAGGTCTTCTATTT+TGG | - | chr6.2:38206314-38206333 | MS.gene98927:CDS | 30.0% |
| !! | CACAACATCTTAAGTTGATT+GGG | - | chr6.2:38206211-38206230 | MS.gene98927:CDS | 30.0% |
| !!! | TATTTTGGTCAATAGTTGTC+AGG | - | chr6.2:38206329-38206348 | MS.gene98927:CDS | 30.0% |
| CTGCATCTAAGCCTATTAAT+TGG | + | chr6.2:38206119-38206138 | None:intergenic | 35.0% | |
| GCAAATGTAGCCCAATTAAT+AGG | - | chr6.2:38206105-38206124 | MS.gene98927:CDS | 35.0% | |
| ! | TATCTTTATTTGCTCGCCAT+GGG | - | chr6.2:38206360-38206379 | MS.gene98927:CDS | 35.0% |
| !! | AACTAGAAGATGCTTTGAGA+AGG | - | chr6.2:38206301-38206320 | MS.gene98927:CDS | 35.0% |
| !! | GCACAACATCTTAAGTTGAT+TGG | - | chr6.2:38206210-38206229 | MS.gene98927:CDS | 35.0% |
| ATAAACAACGTTCCATCCCA+TGG | + | chr6.2:38206379-38206398 | None:intergenic | 40.0% | |
| CATGGCGAGCAAATAAAGAT+AGG | + | chr6.2:38206361-38206380 | None:intergenic | 40.0% | |
| GCATCTTCATGGGAACAATT+TGG | - | chr6.2:38206075-38206094 | MS.gene98927:CDS | 40.0% | |
| ! | CTATCTTTATTTGCTCGCCA+TGG | - | chr6.2:38206359-38206378 | MS.gene98927:CDS | 40.0% |
| ! | GCATCTTCTAGTTGTTCTAG+AGG | + | chr6.2:38206294-38206313 | None:intergenic | 40.0% |
| CGAAAGCAGCAAATACAGCA+AGG | - | chr6.2:38206160-38206179 | MS.gene98927:CDS | 45.0% | |
| TTTATTTGCTCGCCATGGGA+TGG | - | chr6.2:38206364-38206383 | MS.gene98927:CDS | 45.0% | |
| !! | CTAGTTGTTCTAGAGGCTCT+CGG | + | chr6.2:38206287-38206306 | None:intergenic | 45.0% |
| !! | TAGTTGTTCTAGAGGCTCTC+GGG | + | chr6.2:38206286-38206305 | None:intergenic | 45.0% |
| CTAGAGGCTCTCGGGTTTCA+GGG | + | chr6.2:38206278-38206297 | None:intergenic | 55.0% | |
| TCTAGAGGCTCTCGGGTTTC+AGG | + | chr6.2:38206279-38206298 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 38206072 | 38206452 | 38206072 | ID=MS.gene98927 |
| chr6.2 | mRNA | 38206072 | 38206452 | 38206072 | ID=MS.gene98927.t1;Parent=MS.gene98927 |
| chr6.2 | exon | 38206072 | 38206452 | 38206072 | ID=MS.gene98927.t1.exon1;Parent=MS.gene98927.t1 |
| chr6.2 | CDS | 38206072 | 38206452 | 38206072 | ID=cds.MS.gene98927.t1;Parent=MS.gene98927.t1 |
| Gene Sequence |
| Protein sequence |
