Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99719.t1 | XP_003615940.1 | 88.8 | 160 | 18 | 0 | 1 | 160 | 1 | 160 | 2.30E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99719.t1 | G7K5S3 | 88.8 | 160 | 18 | 0 | 1 | 160 | 1 | 160 | 1.6e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene99719 | 0.81343 | 2.57E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene99719 | 0.830436 | 2.94E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene99719 | 0.803432 | 3.50E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene99719 | 0.833623 | 4.80E-56 | -1.69E-46 |
| MS.gene051043 | MS.gene99719 | 0.800413 | 1.46E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene99719 | 0.81524 | 1.02E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene99719 | 0.832146 | 1.12E-55 | -1.69E-46 |
| MS.gene053931 | MS.gene99719 | 0.817884 | 2.62E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene99719 | 0.821339 | 4.26E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene99719 | 0.805151 | 1.53E-49 | -1.69E-46 |
| MS.gene054413 | MS.gene99719 | 0.814823 | 1.27E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene99719 | 0.81043 | 1.16E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene99719 | 0.808732 | 2.68E-50 | -1.69E-46 |
| MS.gene058781 | MS.gene99719 | 0.80083 | 1.20E-48 | -1.69E-46 |
| MS.gene059146 | MS.gene99719 | 0.807273 | 5.48E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene99719 | -0.801263 | 9.78E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene99719 | 0.893508 | 5.89E-75 | -1.69E-46 |
| MS.gene060933 | MS.gene99719 | 0.804377 | 2.22E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene99719 | 0.816009 | 6.90E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene99719 | 0.820782 | 5.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene99719 | MS.gene004251 | PPI |
| MS.gene99719 | MS.gene54297 | PPI |
| MS.gene99719 | MS.gene01564 | PPI |
| MS.gene99719 | MS.gene002580 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99719.t1 | MTR_5g074290 | 98.125 | 160 | 3 | 0 | 1 | 160 | 1 | 160 | 1.21e-112 | 316 |
| MS.gene99719.t1 | MTR_3g031140 | 65.116 | 172 | 44 | 4 | 1 | 158 | 1 | 170 | 2.44e-67 | 202 |
| MS.gene99719.t1 | MTR_2g016150 | 42.778 | 180 | 80 | 5 | 1 | 157 | 1 | 180 | 7.64e-26 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99719.t1 | AT2G30230 | 56.425 | 179 | 55 | 4 | 1 | 159 | 1 | 176 | 4.75e-58 | 179 |
| MS.gene99719.t1 | AT1G06980 | 59.302 | 172 | 53 | 4 | 1 | 159 | 1 | 168 | 8.55e-58 | 178 |
| MS.gene99719.t1 | AT1G29195 | 39.683 | 189 | 81 | 5 | 1 | 157 | 1 | 188 | 1.78e-34 | 119 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGTATAGTTCATTTAAA+TGG | 0.184956 | 5.2:+62140054 | MS.gene99719:CDS |
| AGAACTTCCCCAGCTGAAAT+TGG | 0.250090 | 5.2:-62140099 | None:intergenic |
| GAATAGCTTAAGGTGTTGTT+TGG | 0.353238 | 5.2:+62139999 | MS.gene99719:CDS |
| GGTTTGCTTAGAACATGATT+TGG | 0.371952 | 5.2:-62140132 | None:intergenic |
| CGAACGACTCCTTCAGAGCT+TGG | 0.393027 | 5.2:-62140153 | None:intergenic |
| ACAGAAACATTGTGAGTCTA+AGG | 0.406561 | 5.2:+62140371 | MS.gene99719:CDS |
| GATCCCATCCACATCGTTAC+CGG | 0.408720 | 5.2:+62140233 | MS.gene99719:CDS |
| AGCCATTTAAATGAACTATA+CGG | 0.418439 | 5.2:-62140056 | None:intergenic |
| GCTTAGAACATGATTTGGAT+TGG | 0.460869 | 5.2:-62140127 | None:intergenic |
| CAATAATGGGGAATAGCTTA+AGG | 0.463109 | 5.2:+62139989 | None:intergenic |
| ATTACACGTCCAATTTCAGC+TGG | 0.481683 | 5.2:+62140090 | MS.gene99719:CDS |
| ATCGCCGGCATAGCCGTAGC+TGG | 0.494180 | 5.2:+62140415 | MS.gene99719:CDS |
| CAAGATGAGGTTGCCAGCTA+CGG | 0.497711 | 5.2:-62140428 | None:intergenic |
| TTCTCCGGTAACGATGTGGA+TGG | 0.498133 | 5.2:-62140237 | None:intergenic |
| GTTGCCAGCTACGGCTATGC+CGG | 0.501915 | 5.2:-62140419 | None:intergenic |
| CTAAGCAAACCAAGCTCTGA+AGG | 0.503856 | 5.2:+62140144 | MS.gene99719:CDS |
| CCGGCGAAAGGATGAGAATC+CGG | 0.510917 | 5.2:-62140176 | None:intergenic |
| CTTCTTGCTCTTCTCTTCAG+AGG | 0.524828 | 5.2:-62140322 | None:intergenic |
| CCGGATTCTCATCCTTTCGC+CGG | 0.527436 | 5.2:+62140176 | MS.gene99719:CDS |
| AAAGCTCGAGGCGAGATCGC+CGG | 0.545398 | 5.2:+62140400 | MS.gene99719:CDS |
| TCTATGATACTTTCAAGATG+AGG | 0.552754 | 5.2:-62140441 | None:intergenic |
| TCTCCGGTAACGATGTGGAT+GGG | 0.553981 | 5.2:-62140236 | None:intergenic |
| GCTCTGAAGGAGTCGTTCGC+CGG | 0.557154 | 5.2:+62140157 | MS.gene99719:CDS |
| TGAGCTCAGTCTCCGGCGAA+AGG | 0.557541 | 5.2:-62140188 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.558760 | 5.2:-62140195 | None:intergenic |
| TTGGCTTGTGTTCTTCCATG+TGG | 0.602379 | 5.2:+62140018 | MS.gene99719:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.612896 | 5.2:+62140195 | MS.gene99719:CDS |
| TACACGTCCAATTTCAGCTG+GGG | 0.613892 | 5.2:+62140092 | MS.gene99719:CDS |
| TTACACGTCCAATTTCAGCT+GGG | 0.627095 | 5.2:+62140091 | MS.gene99719:CDS |
| ATCAAGTCTAGTGCTCCACA+TGG | 0.636272 | 5.2:-62140033 | None:intergenic |
| CTAAGGAGATGAAAAGCTCG+AGG | 0.669226 | 5.2:+62140388 | MS.gene99719:CDS |
| GAGGGTCAGAAGAAGCGTCG+CGG | 0.726452 | 5.2:+62140266 | MS.gene99719:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCCATTTAAATGAACTATA+CGG | - | chr5.2:62140059-62140078 | None:intergenic | 25.0% |
| ! | ATCCGTATAGTTCATTTAAA+TGG | + | chr5.2:62140054-62140073 | MS.gene99719:CDS | 25.0% |
| TCTATGATACTTTCAAGATG+AGG | - | chr5.2:62140444-62140463 | None:intergenic | 30.0% | |
| ACAGAAACATTGTGAGTCTA+AGG | + | chr5.2:62140371-62140390 | MS.gene99719:CDS | 35.0% | |
| ! | GCTTAGAACATGATTTGGAT+TGG | - | chr5.2:62140130-62140149 | None:intergenic | 35.0% |
| ! | GGTTTGCTTAGAACATGATT+TGG | - | chr5.2:62140135-62140154 | None:intergenic | 35.0% |
| !! | GAATAGCTTAAGGTGTTGTT+TGG | + | chr5.2:62139999-62140018 | MS.gene99719:CDS | 35.0% |
| ATTACACGTCCAATTTCAGC+TGG | + | chr5.2:62140090-62140109 | MS.gene99719:CDS | 40.0% | |
| CATCGTTACCGGAGAAAAAA+CGG | + | chr5.2:62140244-62140263 | MS.gene99719:CDS | 40.0% | |
| TTACACGTCCAATTTCAGCT+GGG | + | chr5.2:62140091-62140110 | MS.gene99719:CDS | 40.0% | |
| !!! | TTTTTTCTCCGGTAACGATG+TGG | - | chr5.2:62140244-62140263 | None:intergenic | 40.0% |
| AGAACTTCCCCAGCTGAAAT+TGG | - | chr5.2:62140102-62140121 | None:intergenic | 45.0% | |
| ATCAAGTCTAGTGCTCCACA+TGG | - | chr5.2:62140036-62140055 | None:intergenic | 45.0% | |
| CTAAGGAGATGAAAAGCTCG+AGG | + | chr5.2:62140388-62140407 | MS.gene99719:CDS | 45.0% | |
| CTTCTTGCTCTTCTCTTCAG+AGG | - | chr5.2:62140325-62140344 | None:intergenic | 45.0% | |
| GTTACCGGAGAAAAAACGGA+GGG | + | chr5.2:62140248-62140267 | MS.gene99719:CDS | 45.0% | |
| TACACGTCCAATTTCAGCTG+GGG | + | chr5.2:62140092-62140111 | MS.gene99719:CDS | 45.0% | |
| ! | CTAAGCAAACCAAGCTCTGA+AGG | + | chr5.2:62140144-62140163 | MS.gene99719:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCATG+TGG | + | chr5.2:62140018-62140037 | MS.gene99719:CDS | 45.0% |
| CAAGATGAGGTTGCCAGCTA+CGG | - | chr5.2:62140431-62140450 | None:intergenic | 50.0% | |
| CGTTACCGGAGAAAAAACGG+AGG | + | chr5.2:62140247-62140266 | MS.gene99719:CDS | 50.0% | |
| GATCCCATCCACATCGTTAC+CGG | + | chr5.2:62140233-62140252 | MS.gene99719:CDS | 50.0% | |
| TCTCCGGTAACGATGTGGAT+GGG | - | chr5.2:62140239-62140258 | None:intergenic | 50.0% | |
| TTCTCCGGTAACGATGTGGA+TGG | - | chr5.2:62140240-62140259 | None:intergenic | 50.0% | |
| !!! | CTGACCCTCCGTTTTTTCTC+CGG | - | chr5.2:62140255-62140274 | None:intergenic | 50.0% |
| CCGGATTCTCATCCTTTCGC+CGG | + | chr5.2:62140176-62140195 | MS.gene99719:CDS | 55.0% | |
| CCGGCGAAAGGATGAGAATC+CGG | - | chr5.2:62140179-62140198 | None:intergenic | 55.0% | |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr5.2:62140198-62140217 | None:intergenic | 55.0% | |
| CGAACGACTCCTTCAGAGCT+TGG | - | chr5.2:62140156-62140175 | None:intergenic | 55.0% | |
| AAAGCTCGAGGCGAGATCGC+CGG | + | chr5.2:62140400-62140419 | MS.gene99719:CDS | 60.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr5.2:62140195-62140214 | MS.gene99719:CDS | 60.0% | |
| GTTGCCAGCTACGGCTATGC+CGG | - | chr5.2:62140422-62140441 | None:intergenic | 60.0% | |
| TGAGCTCAGTCTCCGGCGAA+AGG | - | chr5.2:62140191-62140210 | None:intergenic | 60.0% | |
| !! | GAGGGTCAGAAGAAGCGTCG+CGG | + | chr5.2:62140266-62140285 | MS.gene99719:CDS | 60.0% |
| !! | GCTCTGAAGGAGTCGTTCGC+CGG | + | chr5.2:62140157-62140176 | MS.gene99719:CDS | 60.0% |
| ATCGCCGGCATAGCCGTAGC+TGG | + | chr5.2:62140415-62140434 | MS.gene99719:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 62139994 | 62140476 | 62139994 | ID=MS.gene99719 |
| chr5.2 | mRNA | 62139994 | 62140476 | 62139994 | ID=MS.gene99719.t1;Parent=MS.gene99719 |
| chr5.2 | exon | 62139994 | 62140476 | 62139994 | ID=MS.gene99719.t1.exon1;Parent=MS.gene99719.t1 |
| chr5.2 | CDS | 62139994 | 62140476 | 62139994 | ID=cds.MS.gene99719.t1;Parent=MS.gene99719.t1 |
| Gene Sequence |
| Protein sequence |
