Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001803.01.T01 | GAU25257.1 | 67.01 | 97 | 24 | 2 | 1 | 90 | 1 | 96 | 4.93E-33 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001803.01.T01 | A0A2Z6N7M3 | 67.010 | 97 | 24 | 2 | 1 | 90 | 1 | 96 | 2.35e-33 | 120 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001801.01 | MsG0180001803.01 | 0.921180 | 4.976992e-88 | 1.752925e-83 |
| MsG0180001802.01 | MsG0180001803.01 | 0.931994 | 1.654919e-94 | 1.081996e-89 |
| MsG0180001803.01 | MsG0180002935.01 | 0.810721 | 1.001239e-50 | 6.081270e-48 |
| MsG0180001803.01 | MsG0180005707.01 | 0.821252 | 4.455744e-53 | 3.606211e-50 |
| MsG0180001803.01 | MsG0180005720.01 | 0.851222 | 1.021352e-60 | 2.052945e-57 |
| MsG0180001803.01 | MsG0180005817.01 | -0.811350 | 7.315801e-51 | 4.517754e-48 |
| MsG0180001803.01 | MsG0280006485.01 | 0.842771 | 2.109314e-58 | 3.231400e-55 |
| MsG0180001803.01 | MsG0280008659.01 | 0.811855 | 5.681182e-51 | 3.555622e-48 |
| MsG0180001803.01 | MsG0280011299.01 | -0.800382 | 1.479540e-48 | 6.879551e-46 |
| MsG0180001803.01 | MsG0380017582.01 | 0.834499 | 2.892901e-56 | 3.439479e-53 |
| MsG0180001803.01 | MsG0380017590.01 | 0.806070 | 9.834604e-50 | 5.288066e-47 |
| MsG0180001803.01 | MsG0480019923.01 | 0.802091 | 6.611870e-49 | 3.210349e-46 |
| MsG0180001803.01 | MsG0480021604.01 | 0.804717 | 1.889157e-49 | 9.811330e-47 |
| MsG0180001803.01 | MsG0480021912.01 | -0.806497 | 7.993502e-50 | 4.345155e-47 |
| MsG0180001803.01 | MsG0580025117.01 | 0.828334 | 9.526565e-55 | 9.439980e-52 |
| MsG0180001803.01 | MsG0580025663.01 | -0.800949 | 1.133416e-48 | 5.346635e-46 |
| MsG0180001803.01 | MsG0580027036.01 | 0.805721 | 1.164517e-49 | 6.206217e-47 |
| MsG0180001803.01 | MsG0680035841.01 | 0.833224 | 6.031189e-56 | 6.901362e-53 |
| MsG0180001803.01 | MsG0780037751.01 | 0.825585 | 4.325946e-54 | 3.959546e-51 |
| MsG0180001803.01 | MsG0780040977.01 | 0.812612 | 3.883048e-51 | 2.480293e-48 |
| MsG0180001803.01 | MsG0880045804.01 | 0.817090 | 3.948059e-52 | 2.847947e-49 |
| MsG0180001803.01 | MsG0880046463.01 | 0.816175 | 6.331691e-52 | 4.454448e-49 |
| MsG0180001803.01 | MsG0880046838.01 | -0.819567 | 1.085331e-52 | 8.382168e-50 |
| MsG0180001803.01 | MsG0880047714.01 | 0.805632 | 1.215493e-49 | 6.462838e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001803.01.T01 | MTR_1g043320 | 82.353 | 102 | 6 | 2 | 1 | 90 | 1 | 102 | 1.03e-29 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACATAGTGAGATGTATT+TGG | 0.212305 | 1:-27237828 | None:intergenic |
| ATCTCACTATGTCTTCTATT+AGG | 0.217073 | 1:+27237836 | MsG0180001803.01.T01:CDS |
| GTAGGAGGCCAGTGTTTCTT+AGG | 0.267431 | 1:-27237944 | None:intergenic |
| ACTTCTCTAGCAGATCTTTC+AGG | 0.273797 | 1:+27237878 | MsG0180001803.01.T01:CDS |
| GGGATTGTTTGGTCCACTTT+TGG | 0.317502 | 1:-27237989 | None:intergenic |
| TCTCACTATGTCTTCTATTA+GGG | 0.339738 | 1:+27237837 | MsG0180001803.01.T01:CDS |
| TGGAAGAAGCATCCTCCTTC+AGG | 0.366754 | 1:+27238064 | MsG0180001803.01.T01:CDS |
| AGACATAGTGAGATGTATTT+GGG | 0.372850 | 1:-27237827 | None:intergenic |
| CTTCTCTAGCAGATCTTTCA+GGG | 0.458151 | 1:+27237879 | MsG0180001803.01.T01:CDS |
| ACAAAATGACCTTCCAAAAG+TGG | 0.469837 | 1:+27237976 | MsG0180001803.01.T01:CDS |
| TTCCATGGACGCTTGTGGTG+AGG | 0.521261 | 1:-27238046 | None:intergenic |
| GAAGGAGGATGCTTCTTCCA+TGG | 0.522552 | 1:-27238061 | None:intergenic |
| GGAGGCCAGTGTTTCTTAGG+TGG | 0.526873 | 1:-27237941 | None:intergenic |
| TCAGGGGATGAATCCTCTGT+GGG | 0.528609 | 1:+27237896 | MsG0180001803.01.T01:CDS |
| CCTCTGTGGGTGTTAATGCT+TGG | 0.545279 | 1:+27237909 | MsG0180001803.01.T01:CDS |
| ACAAAACATACAAGCACAAG+AGG | 0.560306 | 1:+27238012 | MsG0180001803.01.T01:CDS |
| ATTAATCAATTTCCTGAAGG+AGG | 0.566620 | 1:-27238076 | None:intergenic |
| TTCAGGGGATGAATCCTCTG+TGG | 0.567230 | 1:+27237895 | MsG0180001803.01.T01:CDS |
| AGCCTCACCACAAGCGTCCA+TGG | 0.568001 | 1:+27238044 | MsG0180001803.01.T01:CDS |
| ATGATTAATCAATTTCCTGA+AGG | 0.570240 | 1:-27238079 | None:intergenic |
| ATAAGCCACCTAAGAAACAC+TGG | 0.600071 | 1:+27237936 | MsG0180001803.01.T01:CDS |
| GCTTCTTCCATGGACGCTTG+TGG | 0.612657 | 1:-27238051 | None:intergenic |
| TGAAAGATCTGCTAGAGAAG+TGG | 0.631719 | 1:-27237876 | None:intergenic |
| AAGATCTGCTAGAGAAGTGG+TGG | 0.634386 | 1:-27237873 | None:intergenic |
| TTCTCTAGCAGATCTTTCAG+GGG | 0.636352 | 1:+27237880 | MsG0180001803.01.T01:CDS |
| CCAAGCATTAACACCCACAG+AGG | 0.671261 | 1:-27237909 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGACATAGTGAGATGTATT+TGG | - | Chr1:27237831-27237850 | None:intergenic | 30.0% | |
| AGACATAGTGAGATGTATTT+GGG | - | Chr1:27237830-27237849 | None:intergenic | 30.0% | |
| ATCTCACTATGTCTTCTATT+AGG | + | Chr1:27237836-27237855 | MsG0180001803.01.T01:CDS | 30.0% | |
| TCTCACTATGTCTTCTATTA+GGG | + | Chr1:27237837-27237856 | MsG0180001803.01.T01:CDS | 30.0% | |
| ACAAAACATACAAGCACAAG+AGG | + | Chr1:27238012-27238031 | MsG0180001803.01.T01:CDS | 35.0% | |
| ACAAAATGACCTTCCAAAAG+TGG | + | Chr1:27237976-27237995 | MsG0180001803.01.T01:CDS | 35.0% | |
| ! | CTTGTGCTTGTATGTTTTGT+GGG | - | Chr1:27238012-27238031 | None:intergenic | 35.0% |
| ! | GTATGTTTTGTGGGATTGTT+TGG | - | Chr1:27238003-27238022 | None:intergenic | 35.0% |
| ! | TCATTTTGTGTAGTTGTGGT+AGG | - | Chr1:27237965-27237984 | None:intergenic | 35.0% |
| ! | TCTTGTGCTTGTATGTTTTG+TGG | - | Chr1:27238013-27238032 | None:intergenic | 35.0% |
| !! | AAGGTCATTTTGTGTAGTTG+TGG | - | Chr1:27237969-27237988 | None:intergenic | 35.0% |
| ACTTCTCTAGCAGATCTTTC+AGG | + | Chr1:27237878-27237897 | MsG0180001803.01.T01:CDS | 40.0% | |
| ATAAGCCACCTAAGAAACAC+TGG | + | Chr1:27237936-27237955 | MsG0180001803.01.T01:CDS | 40.0% | |
| CTTCTCTAGCAGATCTTTCA+GGG | + | Chr1:27237879-27237898 | MsG0180001803.01.T01:CDS | 40.0% | |
| TGAAAGATCTGCTAGAGAAG+TGG | - | Chr1:27237879-27237898 | None:intergenic | 40.0% | |
| TTCTCTAGCAGATCTTTCAG+GGG | + | Chr1:27237880-27237899 | MsG0180001803.01.T01:CDS | 40.0% | |
| ! | TTTTGTGTAGTTGTGGTAGG+AGG | - | Chr1:27237962-27237981 | None:intergenic | 40.0% |
| !! | TTGTTTGGTCCACTTTTGGA+AGG | - | Chr1:27237988-27238007 | None:intergenic | 40.0% |
| AAGATCTGCTAGAGAAGTGG+TGG | - | Chr1:27237876-27237895 | None:intergenic | 45.0% | |
| ! | GGGATTGTTTGGTCCACTTT+TGG | - | Chr1:27237992-27238011 | None:intergenic | 45.0% |
| CCAAGCATTAACACCCACAG+AGG | - | Chr1:27237912-27237931 | None:intergenic | 50.0% | |
| CCTCTGTGGGTGTTAATGCT+TGG | + | Chr1:27237909-27237928 | MsG0180001803.01.T01:CDS | 50.0% | |
| GAAGGAGGATGCTTCTTCCA+TGG | - | Chr1:27238064-27238083 | None:intergenic | 50.0% | |
| GTAGGAGGCCAGTGTTTCTT+AGG | - | Chr1:27237947-27237966 | None:intergenic | 50.0% | |
| TCAGGGGATGAATCCTCTGT+GGG | + | Chr1:27237896-27237915 | MsG0180001803.01.T01:CDS | 50.0% | |
| TTCAGGGGATGAATCCTCTG+TGG | + | Chr1:27237895-27237914 | MsG0180001803.01.T01:CDS | 50.0% | |
| !! | TGGAAGAAGCATCCTCCTTC+AGG | + | Chr1:27238064-27238083 | MsG0180001803.01.T01:CDS | 50.0% |
| GCTTCTTCCATGGACGCTTG+TGG | - | Chr1:27238054-27238073 | None:intergenic | 55.0% | |
| GGAGGCCAGTGTTTCTTAGG+TGG | - | Chr1:27237944-27237963 | None:intergenic | 55.0% | |
| TTCCATGGACGCTTGTGGTG+AGG | - | Chr1:27238049-27238068 | None:intergenic | 55.0% | |
| AGCCTCACCACAAGCGTCCA+TGG | + | Chr1:27238044-27238063 | MsG0180001803.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 27237821 | 27238093 | 27237821 | ID=MsG0180001803.01;Name=MsG0180001803.01 |
| Chr1 | mRNA | 27237821 | 27238093 | 27237821 | ID=MsG0180001803.01.T01;Parent=MsG0180001803.01;Name=MsG0180001803.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|90 |
| Chr1 | exon | 27237821 | 27238093 | 27237821 | ID=MsG0180001803.01.T01:exon:34845;Parent=MsG0180001803.01.T01 |
| Chr1 | CDS | 27237821 | 27238093 | 27237821 | ID=MsG0180001803.01.T01:cds;Parent=MsG0180001803.01.T01 |
| Gene Sequence |
| Protein sequence |
