AlfalfaGEDB | MsG0180001801.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001801.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001801.01.T01	RHN78606.1	96.078	102	4	0	1	102	13	114	2.66E-63	197

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001801.01.T01	A0A396JK39	96.078	102	4	0	1	102	13	114	1.27e-63	197

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049042.01	MsG0180001801.01	0.819904	9.091635e-53	7.087927e-50
MsG0180001079.01	MsG0180001801.01	0.801774	7.682548e-49	3.700167e-46
MsG0180001801.01	MsG0180001802.01	0.990976	2.918180e-185	2.110488e-178
MsG0180001801.01	MsG0180001803.01	0.921180	4.976992e-88	1.752925e-83
MsG0180001801.01	MsG0180002935.01	0.807734	4.373971e-50	2.455104e-47
MsG0180001801.01	MsG0180004491.01	0.802740	4.859527e-49	2.398926e-46
MsG0180001801.01	MsG0180005720.01	0.807651	4.554037e-50	2.550562e-47
MsG0180001801.01	MsG0180005817.01	-0.837484	5.058082e-57	6.579938e-54
MsG0180001801.01	MsG0280006485.01	0.874165	8.354394e-68	3.722413e-64
MsG0180001801.01	MsG0280006932.01	0.810587	1.070411e-50	6.478017e-48
MsG0180001801.01	MsG0280007759.01	0.806582	7.669179e-50	4.177914e-47
MsG0180001801.01	MsG0280007760.01	0.806978	6.324846e-50	3.480972e-47
MsG0180001801.01	MsG0280008659.01	0.821676	3.557364e-53	2.913528e-50
MsG0180001801.01	MsG0280009416.01	0.844150	9.036036e-59	1.445743e-55
MsG0180001801.01	MsG0280009656.01	0.815214	1.036692e-51	7.103202e-49
MsG0180001801.01	MsG0280010296.01	0.831042	2.088174e-55	2.239527e-52
MsG0180001801.01	MsG0280010463.01	0.800065	1.716323e-48	7.916460e-46
MsG0180001801.01	MsG0280010502.01	0.835259	1.862578e-56	2.265623e-53
MsG0180001801.01	MsG0380011638.01	0.819634	1.047987e-52	8.108580e-50
MsG0180001801.01	MsG0380013409.01	0.800994	1.109931e-48	5.242006e-46
MsG0180001801.01	MsG0380015115.01	0.815382	9.511509e-52	6.547764e-49
MsG0180001801.01	MsG0380017582.01	0.859832	3.148637e-63	8.430338e-60
MsG0180001801.01	MsG0380017590.01	0.819670	1.027914e-52	7.961533e-50
MsG0180001801.01	MsG0480018440.01	0.812457	4.199176e-51	2.671406e-48
MsG0180001801.01	MsG0480019923.01	0.803306	3.711802e-49	1.858913e-46
MsG0180001801.01	MsG0480019954.01	0.824532	7.672307e-54	6.814420e-51
MsG0180001801.01	MsG0480021912.01	-0.815344	9.702512e-52	6.672184e-49
MsG0180001801.01	MsG0480022735.01	0.811785	5.884132e-51	3.675842e-48
MsG0180001801.01	MsG0580025117.01	0.823222	1.556147e-53	1.331729e-50
MsG0180001801.01	MsG0580027036.01	0.809055	2.286476e-50	1.328767e-47
MsG0180001801.01	MsG0580028023.01	0.818608	1.793993e-52	1.349103e-49
MsG0180001801.01	MsG0680035841.01	0.838921	2.157331e-57	2.932264e-54
MsG0180001801.01	MsG0680035842.01	0.814248	1.696737e-51	1.132660e-48
MsG0180001801.01	MsG0780037751.01	0.839359	1.660769e-57	2.288069e-54
MsG0180001801.01	MsG0780039190.01	0.805590	1.240201e-49	6.587058e-47
MsG0180001801.01	MsG0780040480.01	0.823834	1.118951e-53	9.743522e-51
MsG0180001801.01	MsG0780040977.01	0.800252	1.572422e-48	7.287190e-46
MsG0180001801.01	MsG0880043447.01	0.809923	1.488121e-50	8.850222e-48
MsG0180001801.01	MsG0880044855.01	0.826001	3.447237e-54	3.192963e-51
MsG0180001801.01	MsG0880045804.01	0.808041	3.763210e-50	2.129292e-47
MsG0180001801.01	MsG0880046463.01	0.838512	2.752052e-57	3.694434e-54
MsG0180001801.01	MsG0880047714.01	0.801532	8.611865e-49	4.122562e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001801.01.T01	MTR_1g043320	96.078	102	4	0	1	102	1	102	4.79e-67	196

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGTGGCCAGTGTTTCTT+AGG	0.235134	1:-27204928	None:intergenic
ACTTCTCTAGCAGATCTTTC+AGG	0.273797	1:+27204862	MsG0180001801.01.T01:CDS
AAGACACAGTGAGATGTATT+TGG	0.277898	1:-27204812	None:intergenic
GGGATTGTTTGGTCCACTTT+TGG	0.317502	1:-27204991	None:intergenic
GTAGGAGGCCAGTGTTTCTT+TGG	0.327069	1:-27204946	None:intergenic
TGGAAGAAGCATCCTCCTTC+AGG	0.366754	1:+27205084	MsG0180001801.01.T01:CDS
ATCTCACTGTGTCTTCTACT+AGG	0.408144	1:+27204820	MsG0180001801.01.T01:CDS
AGACACAGTGAGATGTATTT+GGG	0.409412	1:-27204811	None:intergenic
CTTCTCTAGCAGATCTTTCA+GGG	0.458151	1:+27204863	MsG0180001801.01.T01:CDS
GGAGGCCAGTGTTTCTTTGG+TGG	0.468858	1:-27204943	None:intergenic
CTTGTGACTATCCTCAACTA+TGG	0.473523	1:-27205043	None:intergenic
ACAAAATGATCTTCCAAAAG+TGG	0.476864	1:+27204978	MsG0180001801.01.T01:CDS
GAAGGAGGATGCTTCTTCCA+TGG	0.522552	1:-27205081	None:intergenic
TCTCACTGTGTCTTCTACTA+GGG	0.525074	1:+27204821	MsG0180001801.01.T01:CDS
GACTATCCTCAACTATGGTA+TGG	0.539163	1:-27205038	None:intergenic
CCTCTGTGAGTGTTAATGCT+TGG	0.552898	1:+27204893	MsG0180001801.01.T01:CDS
ATTAATCAATTTCCTGAAGG+AGG	0.566620	1:-27205096	None:intergenic
AGCATCACCACAAGCGTCCA+TGG	0.568001	1:+27205064	MsG0180001801.01.T01:CDS
ATGATTAATCAATTTCCTGA+AGG	0.570240	1:-27205099	None:intergenic
ACTGGCCACCAAAGAAACAC+TGG	0.593745	1:+27204938	MsG0180001801.01.T01:CDS
GCTTCTTCCATGGACGCTTG+TGG	0.612657	1:-27205071	None:intergenic
ATAAGCAACCTAAGAAACAC+TGG	0.614646	1:+27204920	MsG0180001801.01.T01:CDS
AGAAGACCATACCATAGTTG+AGG	0.630682	1:+27205032	MsG0180001801.01.T01:CDS
TGAAAGATCTGCTAGAGAAG+TGG	0.631719	1:-27204860	None:intergenic
AAGATCTGCTAGAGAAGTGG+TGG	0.634386	1:-27204857	None:intergenic
TTCTCTAGCAGATCTTTCAG+GGG	0.636352	1:+27204864	MsG0180001801.01.T01:CDS
CCAAGCATTAACACTCACAG+AGG	0.666199	1:-27204893	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAAAATGATCTTCCAAAAG+TGG	+	Chr1:27204978-27204997	MsG0180001801.01.T01:CDS	30.0%
!	AAGATCATTTTGTGTAGTTG+TGG	-	Chr1:27204971-27204990	None:intergenic	30.0%
	AAGACACAGTGAGATGTATT+TGG	-	Chr1:27204815-27204834	None:intergenic	35.0%
	AGACACAGTGAGATGTATTT+GGG	-	Chr1:27204814-27204833	None:intergenic	35.0%
	ATAAGCAACCTAAGAAACAC+TGG	+	Chr1:27204920-27204939	MsG0180001801.01.T01:CDS	35.0%
!	TCATTTTGTGTAGTTGTGGT+AGG	-	Chr1:27204967-27204986	None:intergenic	35.0%
!!!	CTTGTGCTTGTTTGTTTTGT+GGG	-	Chr1:27205014-27205033	None:intergenic	35.0%
!!!	GTTTGTTTTGTGGGATTGTT+TGG	-	Chr1:27205005-27205024	None:intergenic	35.0%
!!!	TCTTGTGCTTGTTTGTTTTG+TGG	-	Chr1:27205015-27205034	None:intergenic	35.0%
	ACTTCTCTAGCAGATCTTTC+AGG	+	Chr1:27204862-27204881	MsG0180001801.01.T01:CDS	40.0%
	AGAAGACCATACCATAGTTG+AGG	+	Chr1:27205032-27205051	MsG0180001801.01.T01:CDS	40.0%
	ATCTCACTGTGTCTTCTACT+AGG	+	Chr1:27204820-27204839	MsG0180001801.01.T01:CDS	40.0%
	CTTCTCTAGCAGATCTTTCA+GGG	+	Chr1:27204863-27204882	MsG0180001801.01.T01:CDS	40.0%
	CTTGTGACTATCCTCAACTA+TGG	-	Chr1:27205046-27205065	None:intergenic	40.0%
	GACTATCCTCAACTATGGTA+TGG	-	Chr1:27205041-27205060	None:intergenic	40.0%
	TCTCACTGTGTCTTCTACTA+GGG	+	Chr1:27204821-27204840	MsG0180001801.01.T01:CDS	40.0%
	TGAAAGATCTGCTAGAGAAG+TGG	-	Chr1:27204863-27204882	None:intergenic	40.0%
	TTCTCTAGCAGATCTTTCAG+GGG	+	Chr1:27204864-27204883	MsG0180001801.01.T01:CDS	40.0%
!	TTTTGTGTAGTTGTGGTAGG+AGG	-	Chr1:27204964-27204983	None:intergenic	40.0%
	AAGATCTGCTAGAGAAGTGG+TGG	-	Chr1:27204860-27204879	None:intergenic	45.0%
	CCAAGCATTAACACTCACAG+AGG	-	Chr1:27204896-27204915	None:intergenic	45.0%
	CCTCTGTGAGTGTTAATGCT+TGG	+	Chr1:27204893-27204912	MsG0180001801.01.T01:CDS	45.0%
!	GGGATTGTTTGGTCCACTTT+TGG	-	Chr1:27204994-27205013	None:intergenic	45.0%
!	TTTGGTGGCCAGTGTTTCTT+AGG	-	Chr1:27204931-27204950	None:intergenic	45.0%
	ACTGGCCACCAAAGAAACAC+TGG	+	Chr1:27204938-27204957	MsG0180001801.01.T01:CDS	50.0%
	GAAGGAGGATGCTTCTTCCA+TGG	-	Chr1:27205084-27205103	None:intergenic	50.0%
	GTAGGAGGCCAGTGTTTCTT+TGG	-	Chr1:27204949-27204968	None:intergenic	50.0%
!!	TGGAAGAAGCATCCTCCTTC+AGG	+	Chr1:27205084-27205103	MsG0180001801.01.T01:CDS	50.0%
	AGCATCACCACAAGCGTCCA+TGG	+	Chr1:27205064-27205083	MsG0180001801.01.T01:CDS	55.0%
	GCTTCTTCCATGGACGCTTG+TGG	-	Chr1:27205074-27205093	None:intergenic	55.0%
	GGAGGCCAGTGTTTCTTTGG+TGG	-	Chr1:27204946-27204965	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	27204805	27205113	27204805	ID=MsG0180001801.01;Name=MsG0180001801.01
Chr1	mRNA	27204805	27205113	27204805	ID=MsG0180001801.01.T01;Parent=MsG0180001801.01;Name=MsG0180001801.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|102
Chr1	exon	27204805	27205113	27204805	ID=MsG0180001801.01.T01:exon:34846;Parent=MsG0180001801.01.T01
Chr1	CDS	27204805	27205113	27204805	ID=MsG0180001801.01.T01:cds;Parent=MsG0180001801.01.T01

Gene Sequence	>MsG0180001801.01.T01 ATGTCTCCCAAATACATCTCACTGTGTCTTCTACTAGGGATAGTGCTTCTTGCCACCACTTCTCTAGCAGATCTTTCAGGGGATGAATCCTCTGTGAGTGTTAATGCTTGGAAACATAAGCAACCTAAGAAACACTGGCCACCAAAGAAACACTGGCCTCCTACCACAACTACACAAAATGATCTTCCAAAAGTGGACCAAACAATCCCACAAAACAAACAAGCACAAGAAGACCATACCATAGTTGAGGATAGTCACAAGCATCACCACAAGCGTCCATGGAAGAAGCATCCTCCTTCAGGAAATTGA
Protein sequence	>MsG0180001801.01.T01 MSPKYISLCLLLGIVLLATTSLADLSGDESSVSVNAWKHKQPKKHWPPKKHWPPTTTTQNDLPKVDQTIPQNKQAQEDHTIVEDSHKHHHKRPWKKHPPSGN*