AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006485.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006485.01.T01 MTR_2g009450 75.676 74 15 3 1 71 1 74 1.24e-17 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAATGAAAATTGCTAAAA+TGG 0.217571 2:+2617408 None:intergenic
CACAAATGACAATGTATATA+TGG 0.287137 2:+2617027 None:intergenic
ATAGATGGCTGAGTAGGTTT+TGG 0.351476 2:+2617218 None:intergenic
TTTGGCAATGCTGGGATTGT+AGG 0.373136 2:+2617236 None:intergenic
CATAGTGATTGGGATTGTAT+AGG 0.385126 2:+2617131 None:intergenic
TGGGATTGTATAGGAATGTT+TGG 0.392653 2:+2617140 None:intergenic
GGGAATGTTGGCATAGTGAT+TGG 0.393983 2:+2617120 None:intergenic
AATTGCTAAAATGGAACTAT+AGG 0.396458 2:+2617417 None:intergenic
TGAGGCAATGTTGGAATTGT+TGG 0.400561 2:+2617278 None:intergenic
TTGAAGGTGGTGGAGAAAGA+AGG 0.429584 2:+2617098 None:intergenic
TATACATTGTCATTTGTGTT+GGG 0.434569 2:-2617022 MsG0280006485.01.T01:CDS
AACACTTGACACGAACTTCA+AGG 0.443717 2:+2617066 None:intergenic
TGAAGGTGGTGGAGAAAGAA+GGG 0.449250 2:+2617099 None:intergenic
GGGATAGTACTAGGAATAGA+TGG 0.460146 2:+2617203 None:intergenic
TGCTATTTCCTTCTCAAACA+TGG 0.466175 2:-2617389 MsG0280006485.01.T01:CDS
ATATACATTGTCATTTGTGT+TGG 0.474128 2:-2617023 MsG0280006485.01.T01:CDS
ACATTTCCTTGAGGCAATGT+TGG 0.483996 2:+2617269 None:intergenic
AAGAAGATTGCGAGCTGCTA+GGG 0.488157 2:+2617360 None:intergenic
TGGAGAAAGAAGGGGAATGT+TGG 0.490440 2:+2617108 None:intergenic
GGAATGTTGGCATAGTGATT+GGG 0.491312 2:+2617121 None:intergenic
TGCTAAAATGGAACTATAGG+AGG 0.524832 2:+2617420 None:intergenic
GAAGAAGATTGCGAGCTGCT+AGG 0.531513 2:+2617359 None:intergenic
TGTATAGGAATGTTTGGAAG+TGG 0.534499 2:+2617146 None:intergenic
CTAGGAATAGATGGCTGAGT+AGG 0.540861 2:+2617212 None:intergenic
GCTGGGATTGTAGGCAAAGG+TGG 0.549782 2:+2617245 None:intergenic
ATAGGAATGTTTGGAAGTGG+TGG 0.557391 2:+2617149 None:intergenic
AATGGAACTATAGGAGGCCA+TGG 0.562785 2:+2617426 None:intergenic
GGTAATGTCCATGTTTGAGA+AGG 0.594515 2:+2617381 None:intergenic
TTGACACGAACTTCAAGGAG+TGG 0.601056 2:+2617071 None:intergenic
GAAGGTGGTGGAGAAAGAAG+GGG 0.618928 2:+2617100 None:intergenic
ACAATTCCAACATTGCCTCA+AGG 0.633830 2:-2617275 MsG0280006485.01.T01:CDS
AAAGGTGGAACATTTCCTTG+AGG 0.706888 2:+2617260 None:intergenic
AATGCTGGGATTGTAGGCAA+AGG 0.711188 2:+2617242 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGCTAAAATGGAACTAT+AGG + Chr2:2617029-2617048 None:intergenic 25.0%
! AGCAATGAAAATTGCTAAAA+TGG + Chr2:2617038-2617057 None:intergenic 25.0%
! CACAAATGACAATGTATATA+TGG + Chr2:2617419-2617438 None:intergenic 25.0%
!! ATATACATTGTCATTTGTGT+TGG - Chr2:2617420-2617439 MsG0280006485.01.T01:CDS 25.0%
!! TATACATTGTCATTTGTGTT+GGG - Chr2:2617421-2617440 MsG0280006485.01.T01:CDS 25.0%
AATGTTGGAATTGTTGGCAA+AGG + Chr2:2617162-2617181 None:intergenic 35.0%
CATAGTGATTGGGATTGTAT+AGG + Chr2:2617315-2617334 None:intergenic 35.0%
GTGGAATAGTTGGAATTGTA+GGG + Chr2:2617263-2617282 None:intergenic 35.0%
TGCTAAAATGGAACTATAGG+AGG + Chr2:2617026-2617045 None:intergenic 35.0%
TGGGATTGTATAGGAATGTT+TGG + Chr2:2617306-2617325 None:intergenic 35.0%
TGTATAGGAATGTTTGGAAG+TGG + Chr2:2617300-2617319 None:intergenic 35.0%
TGTGGAATAGTTGGAATTGT+AGG + Chr2:2617264-2617283 None:intergenic 35.0%
! AGTTCGTGTCAAGTGTTTTA+TGG - Chr2:2617383-2617402 MsG0280006485.01.T01:CDS 35.0%
!! TGCTATTTCCTTCTCAAACA+TGG - Chr2:2617054-2617073 MsG0280006485.01.T01:CDS 35.0%
!!! AATGTTGGCAATGTTGGTTT+TGG + Chr2:2617135-2617154 None:intergenic 35.0%
!!! AATGTTGGTTTTGGAAGTGT+TGG + Chr2:2617126-2617145 None:intergenic 35.0%
!!! TTCAAGGAGTGGTTTTTTGA+AGG + Chr2:2617364-2617383 None:intergenic 35.0%
AAAGGTGGAACATTTCCTTG+AGG + Chr2:2617186-2617205 None:intergenic 40.0%
AACACTTGACACGAACTTCA+AGG + Chr2:2617380-2617399 None:intergenic 40.0%
ACAATTCCAACATTGCCTCA+AGG - Chr2:2617168-2617187 MsG0280006485.01.T01:intron 40.0%
ACATTTCCTTGAGGCAATGT+TGG + Chr2:2617177-2617196 None:intergenic 40.0%
AGAGTGAACTGTGGAATAGT+TGG + Chr2:2617273-2617292 None:intergenic 40.0%
ATAGGAATGTTTGGAAGTGG+TGG + Chr2:2617297-2617316 None:intergenic 40.0%
GGAATGTTGGCATAGTGATT+GGG + Chr2:2617325-2617344 None:intergenic 40.0%
GGAATTGTAGGGATAGTACT+AGG + Chr2:2617252-2617271 None:intergenic 40.0%
GGGATAGTACTAGGAATAGA+TGG + Chr2:2617243-2617262 None:intergenic 40.0%
GGTAATGTCCATGTTTGAGA+AGG + Chr2:2617065-2617084 None:intergenic 40.0%
TGAGGCAATGTTGGAATTGT+TGG + Chr2:2617168-2617187 None:intergenic 40.0%
! ATAGATGGCTGAGTAGGTTT+TGG + Chr2:2617228-2617247 None:intergenic 40.0%
!! TTTGGAAGTGTTGGTGTAGT+TGG + Chr2:2617117-2617136 None:intergenic 40.0%
!!! AAGGAGTGGTTTTTTGAAGG+TGG + Chr2:2617361-2617380 None:intergenic 40.0%
AAGAAGATTGCGAGCTGCTA+GGG + Chr2:2617086-2617105 None:intergenic 45.0%
AATGCTGGGATTGTAGGCAA+AGG + Chr2:2617204-2617223 None:intergenic 45.0%
CTAGGAATAGATGGCTGAGT+AGG + Chr2:2617234-2617253 None:intergenic 45.0%
GGGAATGTTGGCATAGTGAT+TGG + Chr2:2617326-2617345 None:intergenic 45.0%
GTTGGAATTGTTGGCAAAGG+TGG + Chr2:2617159-2617178 None:intergenic 45.0%
TGGAGAAAGAAGGGGAATGT+TGG + Chr2:2617338-2617357 None:intergenic 45.0%
TTGACACGAACTTCAAGGAG+TGG + Chr2:2617375-2617394 None:intergenic 45.0%
TTTGGCAATGCTGGGATTGT+AGG + Chr2:2617210-2617229 None:intergenic 45.0%
!! GAGTAGGTTTTGGCAATGCT+GGG + Chr2:2617218-2617237 None:intergenic 45.0%
!! TGAAGGTGGTGGAGAAAGAA+GGG + Chr2:2617347-2617366 None:intergenic 45.0%
!! TGAGTAGGTTTTGGCAATGC+TGG + Chr2:2617219-2617238 None:intergenic 45.0%
!! TTGAAGGTGGTGGAGAAAGA+AGG + Chr2:2617348-2617367 None:intergenic 45.0%
!!! GAGTGGTTTTTTGAAGGTGG+TGG + Chr2:2617358-2617377 None:intergenic 45.0%
GAAGAAGATTGCGAGCTGCT+AGG + Chr2:2617087-2617106 None:intergenic 50.0%
GGTGGCAATGTTGGCAATGT+TGG + Chr2:2617141-2617160 None:intergenic 50.0%
GTTGGCAAAGGTGGCAATGT+TGG + Chr2:2617150-2617169 None:intergenic 50.0%
! AGTGGTGGAAGAGTGAACTG+TGG + Chr2:2617282-2617301 None:intergenic 50.0%
!! GAAGGTGGTGGAGAAAGAAG+GGG + Chr2:2617346-2617365 None:intergenic 50.0%
GCTGGGATTGTAGGCAAAGG+TGG + Chr2:2617201-2617220 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 2617019 2617446 2617019 ID=MsG0280006485.01;Name=MsG0280006485.01
Chr2 mRNA 2617019 2617446 2617019 ID=MsG0280006485.01.T01;Parent=MsG0280006485.01;Name=MsG0280006485.01.T01;_AED=0.29;_eAED=0.57;_QI=0|0|0|1|0|0|3|0|116
Chr2 exon 2617019 2617182 2617019 ID=MsG0280006485.01.T01:exon:520;Parent=MsG0280006485.01.T01
Chr2 exon 2617213 2617300 2617213 ID=MsG0280006485.01.T01:exon:519;Parent=MsG0280006485.01.T01
Chr2 exon 2617348 2617446 2617348 ID=MsG0280006485.01.T01:exon:518;Parent=MsG0280006485.01.T01
Chr2 CDS 2617348 2617446 2617348 ID=MsG0280006485.01.T01:cds;Parent=MsG0280006485.01.T01
Chr2 CDS 2617213 2617300 2617213 ID=MsG0280006485.01.T01:cds;Parent=MsG0280006485.01.T01
Chr2 CDS 2617019 2617182 2617019 ID=MsG0280006485.01.T01:cds;Parent=MsG0280006485.01.T01
Gene Sequence

>MsG0280006485.01.T01

ATGGCCTCCTATAGTTCCATTTTAGCAATTTTCATTGCTATTTCCTTCTCAAACATGGACATTACCCTAGCAGCTCGCAATCTTCTTCAACAAATCCAACCAACAATTCCAACATTGCCTCAAGGAAATGTTCCACCTTTGCCTACAATCCCAGCATTGCCAAAACCTACTCAGCCATCTATTCCTAAGTTCACTCTTCCACCACTTCCAAACATTCCTATACAATCCCAATCACTATGCCAACATTCCCCTTCTTTCTCCACCACCTTCAAAAAACCACTCCTTGAAGTTCGTGTCAAGTGTTTTATGGATATATATTACCATATATACATTGTCATTTGTGTTGGGTAA

Protein sequence

>MsG0280006485.01.T01

MASYSSILAIFIAISFSNMDITLAARNLLQQIQPTIPTLPQGNVPPLPTIPALPKPTQPSIPKFTLPPLPNIPIQSQSLCQHSPSFSTTFKKPLLEVRVKCFMDIYYHIYIVICVG*