AlfalfaGEDB | MsG0180005707.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005707.01

KEGG: -
GO: -
PFAM: PF01657

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005707.01.T01	XP_013469936.1	94.505	91	5	0	1	91	141	231	6.44E-56	183

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005707.01.T01	O23081	42.857	84	47	1	3	85	156	239	6.41E-13	66.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005707.01.T01	G8A0Z3	94.505	91	5	0	1	91	141	231	3.07e-56	183

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001486.01	MsG0180005707.01	0.804961	1.679977e-49	8.780177e-47
MsG0180001803.01	MsG0180005707.01	0.821252	4.455744e-53	3.606211e-50
MsG0180002935.01	MsG0180005707.01	0.822844	1.905832e-53	1.613757e-50
MsG0180003779.01	MsG0180005707.01	-0.814563	1.445298e-51	9.731006e-49
MsG0180005707.01	MsG0180005720.01	0.972835	2.031058e-135	2.456884e-129
MsG0180005707.01	MsG0280007899.01	0.809276	2.049520e-50	1.198175e-47
MsG0180005707.01	MsG0280009656.01	0.804923	1.711117e-49	8.934461e-47
MsG0180005707.01	MsG0280011050.01	0.808660	2.776925e-50	1.596941e-47
MsG0180005707.01	MsG0280011301.01	-0.806426	8.275365e-50	4.490232e-47
MsG0180005707.01	MsG0380012281.01	-0.807544	4.799895e-50	2.680799e-47
MsG0180005707.01	MsG0380016451.01	0.852327	4.964415e-61	1.033731e-57
MsG0180005707.01	MsG0480019923.01	0.822714	2.043120e-53	1.723627e-50
MsG0180005707.01	MsG0480020958.01	0.813632	2.320303e-51	1.523249e-48
MsG0180005707.01	MsG0480022765.01	-0.816659	4.935117e-52	3.518213e-49
MsG0180005707.01	MsG0580025117.01	0.812764	3.597542e-51	2.307035e-48
MsG0180005707.01	MsG0580025420.01	0.841024	6.105430e-58	8.855490e-55
MsG0180005707.01	MsG0580025663.01	-0.805341	1.398831e-49	7.382094e-47
MsG0180005707.01	MsG0680035841.01	0.819288	1.256224e-52	9.626315e-50
MsG0180005707.01	MsG0780037751.01	0.800584	1.346011e-48	6.290620e-46
MsG0180005707.01	MsG0780040034.01	0.813200	2.886544e-51	1.872777e-48
MsG0180005707.01	MsG0780040977.01	0.821962	3.054111e-53	2.521723e-50
MsG0180005707.01	MsG0780041720.01	-0.813214	2.866545e-51	1.860485e-48
MsG0180005707.01	MsG0880043345.01	0.800824	1.202356e-48	5.653455e-46
MsG0180005707.01	MsG0880045804.01	0.805802	1.119750e-49	5.979809e-47
MsG0180005707.01	MsG0880047318.01	-0.802121	6.520619e-49	3.168350e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005707.01.T01	MTR_1g105885	94.505	91	5	0	1	91	141	231	7.80e-60	183
MsG0180005707.01.T01	MTR_1g105860	67.010	97	32	0	1	97	161	257	1.95e-43	142
MsG0180005707.01.T01	MTR_1g105860	67.010	97	32	0	1	97	144	240	1.98e-43	142
MsG0180005707.01.T01	MTR_1g105855	58.947	95	30	1	1	86	161	255	3.00e-32	114
MsG0180005707.01.T01	MTR_1g021638	54.348	92	41	1	1	91	141	232	4.96e-27	100
MsG0180005707.01.T01	MTR_0341s0010	50.000	96	45	2	1	93	141	236	1.18e-25	97.4
MsG0180005707.01.T01	MTR_1g021632	52.222	90	42	1	3	91	143	232	1.23e-25	99.8
MsG0180005707.01.T01	MTR_1g021630	50.000	96	45	2	1	93	141	236	3.82e-25	98.2
MsG0180005707.01.T01	MTR_5g068260	49.438	89	44	1	3	91	138	225	3.06e-22	90.1
MsG0180005707.01.T01	MTR_5g068290	50.000	84	40	2	8	91	22	103	3.77e-22	88.6
MsG0180005707.01.T01	MTR_1g021635	48.889	90	45	1	3	91	143	232	1.01e-21	88.6
MsG0180005707.01.T01	MTR_1g105820	46.667	90	47	1	3	91	142	231	3.84e-21	86.7
MsG0180005707.01.T01	MTR_1g105850	47.253	91	48	0	1	91	147	237	3.86e-21	85.5
MsG0180005707.01.T01	MTR_1g021610	49.438	89	44	1	3	91	145	232	5.95e-21	86.3
MsG0180005707.01.T01	MTR_1g105755	47.191	89	45	1	3	91	142	228	1.48e-20	85.1
MsG0180005707.01.T01	MTR_1g021642	50.575	87	42	1	1	87	156	241	2.15e-20	84.7
MsG0180005707.01.T01	MTR_0341s0020	46.591	88	45	2	7	93	119	205	4.72e-20	83.6
MsG0180005707.01.T01	MTR_1g105750	46.667	90	47	1	3	91	143	232	1.43e-19	82.4
MsG0180005707.01.T01	MTR_1g105840	43.820	89	50	0	3	91	131	219	6.40e-19	80.5
MsG0180005707.01.T01	MTR_5g068250	46.667	90	47	1	2	91	142	230	6.90e-19	80.5
MsG0180005707.01.T01	MTR_1g105800	47.619	84	42	1	8	91	148	229	3.67e-18	78.2
MsG0180005707.01.T01	MTR_5g068190	40.698	86	47	2	3	84	136	221	6.78e-15	68.9
MsG0180005707.01.T01	MTR_1g105700	37.079	89	48	2	7	87	142	230	1.09e-13	65.5
MsG0180005707.01.T01	MTR_5g065130	40.741	81	47	1	3	83	130	209	1.93e-13	64.7
MsG0180005707.01.T01	MTR_1g105710	41.026	78	39	2	17	87	148	225	2.85e-13	64.3
MsG0180005707.01.T01	MTR_5g005530	40.506	79	45	1	9	87	148	224	1.01e-11	60.1
MsG0180005707.01.T01	MTR_6g463660	36.364	99	59	2	1	96	139	236	1.66e-11	59.3
MsG0180005707.01.T01	MTR_6g057680	36.364	99	59	2	1	96	139	236	1.66e-11	59.3
MsG0180005707.01.T01	MTR_5g005450	41.791	67	38	1	18	84	157	222	7.51e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005707.01.T01	AT4G00970	44.872	78	42	1	9	85	162	239	3.88e-14	67.0
MsG0180005707.01.T01	AT4G00970	42.857	84	47	1	3	85	156	239	6.53e-14	66.2
MsG0180005707.01.T01	AT4G38830	43.421	76	41	2	17	91	152	226	3.32e-13	64.3
MsG0180005707.01.T01	AT4G21410	40.845	71	42	0	17	87	157	227	9.06e-12	60.1
MsG0180005707.01.T01	AT4G21410	40.845	71	42	0	17	87	32	102	9.11e-12	60.1
MsG0180005707.01.T01	AT4G21410	40.845	71	42	0	17	87	157	227	1.04e-11	60.1
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	2.42e-11	58.9
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	2.44e-11	58.9
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	2.44e-11	58.9
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	2.44e-11	58.9
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	32	106	3.06e-11	58.5
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	3.29e-11	58.5
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	159	233	3.32e-11	58.5
MsG0180005707.01.T01	AT4G21400	37.333	75	47	0	17	91	133	207	3.59e-11	58.5
MsG0180005707.01.T01	AT3G22060	39.759	83	48	1	8	90	149	229	4.46e-11	57.8

Find 16 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTGTTGGTACAATATTT+TGG	0.116193	1:-95231536	None:intergenic
AGTAGTGATCAAACAATATA+TGG	0.388142	1:+95231440	MsG0180005707.01.T01:CDS
TTAGAGCAAGCTGTTATTTG+AGG	0.415196	1:+95231572	MsG0180005707.01.T01:CDS
AAATTCTACAACCATCTTGT+TGG	0.418580	1:-95231549	None:intergenic
AAATGTGGCCTGCTATAATT+TGG	0.425646	1:+95231285	None:intergenic
ACAGTGTACACCTGATTTGT+CGG	0.437811	1:+95231469	MsG0180005707.01.T01:CDS
CACTGTGAAACCGACAAATC+AGG	0.458317	1:-95231479	None:intergenic
GAATGTGAACAATGCAACTT+TGG	0.483957	1:+95231310	MsG0180005707.01.T01:CDS
TATATTGTTTGATCACTACT+AGG	0.502209	1:-95231437	None:intergenic
TATGCTGCAGGAACTTTGCC+AGG	0.503711	1:+95231413	MsG0180005707.01.T01:CDS
ACATTCCACCAAATTATAGC+AGG	0.535057	1:-95231293	None:intergenic
AAAATATTGTACCAACAAGA+TGG	0.557644	1:+95231538	MsG0180005707.01.T01:CDS
TCACATCGTAAGTATGCTGC+AGG	0.560986	1:+95231401	MsG0180005707.01.T01:CDS
TGTGGCCTGCTATAATTTGG+TGG	0.578852	1:+95231288	MsG0180005707.01.T01:CDS
GGCAGATCAATTCAATAGTG+TGG	0.581827	1:+95231331	MsG0180005707.01.T01:CDS
GTTTGATCACTACTAGGACC+TGG	0.622316	1:-95231431	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTGAATGATTTAAAAAACA+AGG	+	Chr1:95231364-95231383	MsG0180005707.01.T01:CDS	20.0%
!	AAAATATTGTACCAACAAGA+TGG	+	Chr1:95231538-95231557	MsG0180005707.01.T01:CDS	25.0%
!	AGTAGTGATCAAACAATATA+TGG	+	Chr1:95231440-95231459	MsG0180005707.01.T01:CDS	25.0%
!	TATATTGTTTGATCACTACT+AGG	-	Chr1:95231440-95231459	None:intergenic	25.0%
!	TTAAAAAACAAGGCTAGAAA+TGG	+	Chr1:95231374-95231393	MsG0180005707.01.T01:CDS	25.0%
!!	ATCTTGTTGGTACAATATTT+TGG	-	Chr1:95231539-95231558	None:intergenic	25.0%
!!	TCTTGTTGGTACAATATTTT+GGG	-	Chr1:95231538-95231557	None:intergenic	25.0%
	AAATTCTACAACCATCTTGT+TGG	-	Chr1:95231552-95231571	None:intergenic	30.0%
	ACATTCCACCAAATTATAGC+AGG	-	Chr1:95231296-95231315	None:intergenic	35.0%
	GAATGTGAACAATGCAACTT+TGG	+	Chr1:95231310-95231329	MsG0180005707.01.T01:CDS	35.0%
	GGCAGATCAATTCAATAGTG+TGG	+	Chr1:95231331-95231350	MsG0180005707.01.T01:CDS	40.0%
!	ACAGTGTACACCTGATTTGT+CGG	+	Chr1:95231469-95231488	MsG0180005707.01.T01:CDS	40.0%
	CACTGTGAAACCGACAAATC+AGG	-	Chr1:95231482-95231501	None:intergenic	45.0%
	GTTTGATCACTACTAGGACC+TGG	-	Chr1:95231434-95231453	None:intergenic	45.0%
	TCACATCGTAAGTATGCTGC+AGG	+	Chr1:95231401-95231420	MsG0180005707.01.T01:CDS	45.0%
	TGTGGCCTGCTATAATTTGG+TGG	+	Chr1:95231288-95231307	MsG0180005707.01.T01:CDS	45.0%
	TATGCTGCAGGAACTTTGCC+AGG	+	Chr1:95231413-95231432	MsG0180005707.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	95231287	95231592	95231287	ID=MsG0180005707.01;Name=MsG0180005707.01
Chr1	mRNA	95231287	95231592	95231287	ID=MsG0180005707.01.T01;Parent=MsG0180005707.01;Name=MsG0180005707.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|101
Chr1	exon	95231287	95231592	95231287	ID=MsG0180005707.01.T01:exon:19296;Parent=MsG0180005707.01.T01
Chr1	CDS	95231287	95231592	95231287	ID=MsG0180005707.01.T01:cds;Parent=MsG0180005707.01.T01

Gene Sequence	>MsG0180005707.01.T01 ATGTGGCCTGCTATAATTTGGTGGAATGTGAACAATGCAACTTTGGCAGATCAATTCAATAGTGTGGTGAAACAATTGTTGAATGATTTAAAAAACAAGGCTAGAAATGGTGACTCACATCGTAAGTATGCTGCAGGAACTTTGCCAGGTCCTAGTAGTGATCAAACAATATATGGTCTTGTACAGTGTACACCTGATTTGTCGGTTTCACAGTGTGATGACTGTTTGAATGAATCTATTGCTGAAGTCCCAAAATATTGTACCAACAAGATGGTTGTAGAATTTTTAGAGCAAGCTGTTATTTGA
Protein sequence	>MsG0180005707.01.T01 MWPAIIWWNVNNATLADQFNSVVKQLLNDLKNKARNGDSHRKYAAGTLPGPSSDQTIYGLVQCTPDLSVSQCDDCLNESIAEVPKYCTNKMVVEFLEQAVI*