AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008659.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008659.01.T01 MTR_0097s0070 94.527 201 11 0 1 201 1 201 1.37e-132 370
MsG0280008659.01.T01 MTR_8g089300 72.680 194 52 1 1 194 1 193 9.08e-94 272
MsG0280008659.01.T01 MTR_4g118810 36.957 138 81 3 29 163 35 169 7.53e-22 89.0
MsG0280008659.01.T01 MTR_7g108130 31.609 174 112 3 17 189 18 185 5.06e-21 86.3
MsG0280008659.01.T01 MTR_0004s0210 32.927 164 94 2 29 192 10 157 1.31e-20 84.7
MsG0280008659.01.T01 MTR_2g020060 29.268 164 105 3 31 189 27 184 6.05e-15 70.1
MsG0280008659.01.T01 MTR_7g011090 27.419 186 122 3 6 188 10 185 9.22e-14 67.0
MsG0280008659.01.T01 MTR_4g081880 30.189 159 96 4 31 185 33 180 1.10e-11 61.6
MsG0280008659.01.T01 MTR_5g041900 29.677 155 102 1 31 185 32 179 4.13e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008659.01.T01 AT5G44550 59.302 172 70 0 23 194 14 185 2.01e-66 202
MsG0280008659.01.T01 AT4G20390 54.706 170 76 1 22 190 12 181 8.30e-54 171
MsG0280008659.01.T01 AT4G15620 29.032 186 125 4 5 189 10 189 8.66e-19 80.5
MsG0280008659.01.T01 AT4G15630 27.473 182 126 3 9 189 13 189 6.22e-18 78.2
MsG0280008659.01.T01 AT4G15610 31.250 144 92 3 31 172 33 171 6.24e-17 75.9
MsG0280008659.01.T01 AT1G03700 27.439 164 103 3 29 192 11 158 2.31e-16 73.6
MsG0280008659.01.T01 AT5G15290 30.769 143 90 3 31 172 32 166 3.72e-15 70.9
MsG0280008659.01.T01 AT1G14160 27.742 155 105 1 31 185 55 202 6.52e-15 70.5
MsG0280008659.01.T01 AT3G06390 30.827 133 84 3 32 161 42 169 3.27e-14 68.6
MsG0280008659.01.T01 AT4G03540 27.481 131 79 3 44 174 26 140 4.47e-12 62.0

Find 44 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGAGTTTGATTAAATT+TGG 0.215698 2:+36007801 None:intergenic
TATGCAATCCAATACTTTAT+TGG 0.216311 2:+36006735 None:intergenic
GCCCTTACTGCTTCTTTCAT+TGG 0.229079 2:-36006969 MsG0280008659.01.T01:CDS
GCAGAAATAGTAGCAGTTAT+TGG 0.231666 2:+36007823 None:intergenic
CAGAAATAGTAGCAGTTATT+GGG 0.254239 2:+36007824 None:intergenic
AAGTCCTTTGTAATCATATT+GGG 0.274380 2:+36007268 None:intergenic
GTTAAGTGCTTATTACAAAA+TGG 0.283915 2:-36008031 MsG0280008659.01.T01:exon
TACAAAATGGCTTCAAGAAA+TGG 0.318606 2:-36008018 MsG0280008659.01.T01:exon
GAAGTCCTTTGTAATCATAT+TGG 0.328703 2:+36007267 None:intergenic
GGTGCCAACAATAAAACTTT+TGG 0.346894 2:+36007858 None:intergenic
TTTGAAAGCTATTGCAATAG+AGG 0.418883 2:-36006999 MsG0280008659.01.T01:CDS
ATGTCAGCATTGGGAAGAAA+TGG 0.423404 2:-36007059 MsG0280008659.01.T01:CDS
TTGAAAACTTACACAAAAGC+TGG 0.437745 2:+36007784 None:intergenic
TTGAAAGACCAATAAAGTAT+TGG 0.445012 2:-36006743 MsG0280008659.01.T01:three_prime_UTR
ATGCAATCATCACCAAGTTA+TGG 0.467360 2:+36007306 None:intergenic
GGAGCATGGTTAATGCGATT+AGG 0.487055 2:+36006894 None:intergenic
AGCAACATTCATGTCAGCAT+TGG 0.488005 2:-36007069 MsG0280008659.01.T01:CDS
ACTATGGCTCTGGCATCAGC+TGG 0.494373 2:-36007101 MsG0280008659.01.T01:CDS
GCGATTAGGCTTGAGAAGCT+TGG 0.494528 2:+36006908 None:intergenic
GCAACATTCATGTCAGCATT+GGG 0.495269 2:-36007068 MsG0280008659.01.T01:CDS
AAATGCTAGTCTCCATAACT+TGG 0.496794 2:-36007318 MsG0280008659.01.T01:CDS
GGTGAAGTTAACTATGGCTC+TGG 0.500555 2:-36007111 MsG0280008659.01.T01:intron
AACCAATGAAAGAAGCAGTA+AGG 0.506108 2:+36006967 None:intergenic
TGGTAATTCACATGCAAGAT+GGG 0.512674 2:-36007039 MsG0280008659.01.T01:CDS
ATGGTAATTCACATGCAAGA+TGG 0.522902 2:-36007040 MsG0280008659.01.T01:CDS
ATGGAGACTAGCATTTCCAT+TGG 0.523367 2:+36007325 None:intergenic
AGGTTCTTTGTGATAGCCAA+TGG 0.526185 2:-36007341 MsG0280008659.01.T01:CDS
TATATATGTTAATCATATCA+AGG 0.528309 2:+36006867 None:intergenic
CACAAGTGTTGCAGCTGCAG+TGG 0.536886 2:+36007900 None:intergenic
GCTCTGGCATCAGCTGGAGA+TGG 0.540036 2:-36007095 MsG0280008659.01.T01:CDS
TGTTAATCATATCAAGGACT+AGG 0.549879 2:+36006873 None:intergenic
TAACTTGGTGATGATTGCAT+TGG 0.550469 2:-36007303 MsG0280008659.01.T01:CDS
AGGCTTGAGAAGCTTGGCAA+TGG 0.561801 2:+36006914 None:intergenic
CTAAAATTGCAATAAATGCA+AGG 0.578209 2:+36007243 None:intergenic
ACCAATGAAAGAAGCAGTAA+GGG 0.579511 2:+36006968 None:intergenic
AGCTATTGCAATAGAGGTGG+TGG 0.598102 2:-36006993 MsG0280008659.01.T01:CDS
TGCAGCTGCAACACTTGTGA+TGG 0.606205 2:-36007896 MsG0280008659.01.T01:CDS
CATATCAAGGACTAGGAGCA+TGG 0.606251 2:+36006880 None:intergenic
GGACCCCAATATGATTACAA+AGG 0.614918 2:-36007272 MsG0280008659.01.T01:CDS
GAAAGCTATTGCAATAGAGG+TGG 0.619990 2:-36006996 MsG0280008659.01.T01:CDS
AGTTATTGGGGTGTTACCAA+CGG 0.622547 2:+36007837 None:intergenic
AGTCCTTTGTAATCATATTG+GGG 0.628572 2:+36007269 None:intergenic
AGAAATAGTAGCAGTTATTG+GGG 0.633112 2:+36007825 None:intergenic
GGTGAAGAGAAAGTTGAACG+TGG 0.756724 2:-36007997 MsG0280008659.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTTTAATTTTTTCTT+AGG - Chr2:36007591-36007610 MsG0280008659.01.T01:intron 10.0%
!! AATCAATACTACTTATATTT+CGG + Chr2:36007152-36007171 None:intergenic 15.0%
!! TATATATGTTAATCATATCA+AGG + Chr2:36007907-36007926 None:intergenic 15.0%
!! TTATATTAACACAAAATTGT+GGG - Chr2:36007400-36007419 MsG0280008659.01.T01:intron 15.0%
!! TTTATATTAACACAAAATTG+TGG - Chr2:36007399-36007418 MsG0280008659.01.T01:intron 15.0%
!!! TAGAGATATGTTTAATTTTT+TGG - Chr2:36007334-36007353 MsG0280008659.01.T01:CDS 15.0%
!! AAAGAAAAACAAAGAATGAT+TGG - Chr2:36006814-36006833 MsG0280008659.01.T01:three_prime_UTR 20.0%
!! AATATTGAGAAAATAGAGAA+AGG - Chr2:36007258-36007277 MsG0280008659.01.T01:CDS 20.0%
!! TATATTAACACAAAATTGTG+GGG - Chr2:36007401-36007420 MsG0280008659.01.T01:intron 20.0%
!!! ATTTATTGCAATTTTAGACA+TGG - Chr2:36007534-36007553 MsG0280008659.01.T01:intron 20.0%
! AACGAACATAAATTGAAAAG+AGG + Chr2:36007049-36007068 None:intergenic 25.0%
! AAGTCCTTTGTAATCATATT+GGG + Chr2:36007506-36007525 None:intergenic 25.0%
! AATTTCATCGCTTAATTCAA+AGG - Chr2:36007302-36007321 MsG0280008659.01.T01:CDS 25.0%
! CTAAAATTGCAATAAATGCA+AGG + Chr2:36007531-36007550 None:intergenic 25.0%
! GTTAAGTGCTTATTACAAAA+TGG - Chr2:36006740-36006759 MsG0280008659.01.T01:three_prime_UTR 25.0%
! TTGAAAGACCAATAAAGTAT+TGG - Chr2:36008028-36008047 MsG0280008659.01.T01:exon 25.0%
!! ATGATTGCATTGGATATTTT+AGG - Chr2:36007478-36007497 MsG0280008659.01.T01:intron 25.0%
!! ATTGGATTCTTTTATCTCTT+AGG - Chr2:36006832-36006851 MsG0280008659.01.T01:three_prime_UTR 25.0%
!! CTCTATTTTCTCAATATTAG+TGG + Chr2:36007256-36007275 None:intergenic 25.0%
!! TTGGATTCTTTTATCTCTTA+GGG - Chr2:36006833-36006852 MsG0280008659.01.T01:three_prime_UTR 25.0%
!!! ATATTAGTGGTTAATTTGTC+AGG + Chr2:36007243-36007262 None:intergenic 25.0%
!!! CCAAAAAAGTTTTATGTAGA+AGG - Chr2:36007178-36007197 MsG0280008659.01.T01:intron 25.0%
!!! CCTTCTACATAAAACTTTTT+TGG + Chr2:36007181-36007200 None:intergenic 25.0%
!!! TATTAGTGGTTAATTTGTCA+GGG + Chr2:36007242-36007261 None:intergenic 25.0%
AGAAATAGTAGCAGTTATTG+GGG + Chr2:36006949-36006968 None:intergenic 30.0%
AGCTGGAGTTTGATTAAATT+TGG + Chr2:36006973-36006992 None:intergenic 30.0%
AGTCCTTTGTAATCATATTG+GGG + Chr2:36007505-36007524 None:intergenic 30.0%
CAGAAATAGTAGCAGTTATT+GGG + Chr2:36006950-36006969 None:intergenic 30.0%
GAAGTCCTTTGTAATCATAT+TGG + Chr2:36007507-36007526 None:intergenic 30.0%
TACAAAATGGCTTCAAGAAA+TGG - Chr2:36006753-36006772 MsG0280008659.01.T01:three_prime_UTR 30.0%
TGTTAATCATATCAAGGACT+AGG + Chr2:36007901-36007920 None:intergenic 30.0%
TTGAAAACTTACACAAAAGC+TGG + Chr2:36006990-36007009 None:intergenic 30.0%
TTTGAAAGCTATTGCAATAG+AGG - Chr2:36007772-36007791 MsG0280008659.01.T01:intron 30.0%
!! ATTAGTGGTTAATTTGTCAG+GGG + Chr2:36007241-36007260 None:intergenic 30.0%
!!! CAGACCAAAAGTTTTATTGT+TGG - Chr2:36006909-36006928 MsG0280008659.01.T01:CDS 30.0%
!!! TTTTTGGGTGAAGTTAACTA+TGG - Chr2:36007654-36007673 MsG0280008659.01.T01:intron 30.0%
AACCAATGAAAGAAGCAGTA+AGG + Chr2:36007807-36007826 None:intergenic 35.0%
ACCAATGAAAGAAGCAGTAA+GGG + Chr2:36007806-36007825 None:intergenic 35.0%
ATGCAATCATCACCAAGTTA+TGG + Chr2:36007468-36007487 None:intergenic 35.0%
ATGGTAATTCACATGCAAGA+TGG - Chr2:36007731-36007750 MsG0280008659.01.T01:intron 35.0%
GCAGAAATAGTAGCAGTTAT+TGG + Chr2:36006951-36006970 None:intergenic 35.0%
TAGTAGAAGAGGTCGAATAT+CGG + Chr2:36007205-36007224 None:intergenic 35.0%
TGGTAATTCACATGCAAGAT+GGG - Chr2:36007732-36007751 MsG0280008659.01.T01:intron 35.0%
! AAATGCTAGTCTCCATAACT+TGG - Chr2:36007453-36007472 MsG0280008659.01.T01:intron 35.0%
! ACTTTTCTCTGTTTGAGTGT+TGG - Chr2:36007979-36007998 MsG0280008659.01.T01:CDS 35.0%
! GATTCTTTTATCTCTTAGGG+TGG - Chr2:36006836-36006855 MsG0280008659.01.T01:three_prime_UTR 35.0%
! GGTGCCAACAATAAAACTTT+TGG + Chr2:36006916-36006935 None:intergenic 35.0%
!! TAACTTGGTGATGATTGCAT+TGG - Chr2:36007468-36007487 MsG0280008659.01.T01:intron 35.0%
!! TTAGTGGTTAATTTGTCAGG+GGG + Chr2:36007240-36007259 None:intergenic 35.0%
ACAAAATTGTGGGGTGATAC+AGG - Chr2:36007410-36007429 MsG0280008659.01.T01:intron 40.0%
AGCAACATTCATGTCAGCAT+TGG - Chr2:36007702-36007721 MsG0280008659.01.T01:intron 40.0%
AGGTTCTTTGTGATAGCCAA+TGG - Chr2:36007430-36007449 MsG0280008659.01.T01:intron 40.0%
ATGGAGACTAGCATTTCCAT+TGG + Chr2:36007449-36007468 None:intergenic 40.0%
GAAAGCTATTGCAATAGAGG+TGG - Chr2:36007775-36007794 MsG0280008659.01.T01:intron 40.0%
GAGGGAAATGTTAGTAGAAG+AGG + Chr2:36007216-36007235 None:intergenic 40.0%
GCAACATTCATGTCAGCATT+GGG - Chr2:36007703-36007722 MsG0280008659.01.T01:intron 40.0%
GGACCCCAATATGATTACAA+AGG - Chr2:36007499-36007518 MsG0280008659.01.T01:intron 40.0%
! AGTTATTGGGGTGTTACCAA+CGG + Chr2:36006937-36006956 None:intergenic 40.0%
! ATGTCAGCATTGGGAAGAAA+TGG - Chr2:36007712-36007731 MsG0280008659.01.T01:intron 40.0%
!!! AGTTTTATTGTTGGCACCGT+TGG - Chr2:36006918-36006937 MsG0280008659.01.T01:CDS 40.0%
AGCTATTGCAATAGAGGTGG+TGG - Chr2:36007778-36007797 MsG0280008659.01.T01:intron 45.0%
CATATCAAGGACTAGGAGCA+TGG + Chr2:36007894-36007913 None:intergenic 45.0%
GCCCTTACTGCTTCTTTCAT+TGG - Chr2:36007802-36007821 MsG0280008659.01.T01:CDS 45.0%
GGAGCATGGTTAATGCGATT+AGG + Chr2:36007880-36007899 None:intergenic 45.0%
GGTGAAGAGAAAGTTGAACG+TGG - Chr2:36006774-36006793 MsG0280008659.01.T01:three_prime_UTR 45.0%
GGTGAAGTTAACTATGGCTC+TGG - Chr2:36007660-36007679 MsG0280008659.01.T01:intron 45.0%
!! GTTAATTTGTCAGGGGGAGA+GGG + Chr2:36007234-36007253 None:intergenic 45.0%
!!! ATTGATAAATAATATTTTTT+TGG - Chr2:36007638-36007657 MsG0280008659.01.T01:intron 5.0%
!!! TTGATAAATAATATTTTTTT+GGG - Chr2:36007639-36007658 MsG0280008659.01.T01:intron 5.0%
AGGCTTGAGAAGCTTGGCAA+TGG + Chr2:36007860-36007879 None:intergenic 50.0%
GCGATTAGGCTTGAGAAGCT+TGG + Chr2:36007866-36007885 None:intergenic 50.0%
TGCAGCTGCAACACTTGTGA+TGG - Chr2:36006875-36006894 MsG0280008659.01.T01:exon 50.0%
!! GGTTAATTTGTCAGGGGGAG+AGG + Chr2:36007235-36007254 None:intergenic 50.0%
! CACAAGTGTTGCAGCTGCAG+TGG + Chr2:36006874-36006893 None:intergenic 55.0%
!! ACTATGGCTCTGGCATCAGC+TGG - Chr2:36007670-36007689 MsG0280008659.01.T01:intron 55.0%
CGGACCTCCTACTACTCGTG+CGG + Chr2:36007132-36007151 None:intergenic 60.0%
GACCTCCTACTACTCGTGCG+GGG + Chr2:36007130-36007149 None:intergenic 60.0%
GCTCTGGCATCAGCTGGAGA+TGG - Chr2:36007676-36007695 MsG0280008659.01.T01:intron 60.0%
GGACCTCCTACTACTCGTGC+GGG + Chr2:36007131-36007150 None:intergenic 60.0%
CACCCCGCACGAGTAGTAGG+AGG - Chr2:36007125-36007144 MsG0280008659.01.T01:intron 65.0%
CCTACTACTCGTGCGGGGTG+CGG + Chr2:36007125-36007144 None:intergenic 65.0%
CTACTACTCGTGCGGGGTGC+GGG + Chr2:36007124-36007143 None:intergenic 65.0%
TACTACTCGTGCGGGGTGCG+GGG + Chr2:36007123-36007142 None:intergenic 65.0%
CCGCACCCCGCACGAGTAGT+AGG - Chr2:36007122-36007141 MsG0280008659.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 36006738 36008055 36006738 ID=MsG0280008659.01;Name=MsG0280008659.01
Chr2 mRNA 36006738 36008055 36006738 ID=MsG0280008659.01.T01;Parent=MsG0280008659.01;Name=MsG0280008659.01.T01;_AED=0.23;_eAED=0.23;_QI=21|1|1|1|1|1|3|146|201
Chr2 exon 36006738 36007126 36006738 ID=MsG0280008659.01.T01:exon:15456;Parent=MsG0280008659.01.T01
Chr2 exon 36007238 36007361 36007238 ID=MsG0280008659.01.T01:exon:15455;Parent=MsG0280008659.01.T01
Chr2 exon 36007796 36008055 36007796 ID=MsG0280008659.01.T01:exon:15454;Parent=MsG0280008659.01.T01
Chr2 five_prime_UTR 36008035 36008055 36008035 ID=MsG0280008659.01.T01:five_prime_utr;Parent=MsG0280008659.01.T01
Chr2 CDS 36007796 36008034 36007796 ID=MsG0280008659.01.T01:cds;Parent=MsG0280008659.01.T01
Chr2 CDS 36007238 36007361 36007238 ID=MsG0280008659.01.T01:cds;Parent=MsG0280008659.01.T01
Chr2 CDS 36006884 36007126 36006884 ID=MsG0280008659.01.T01:cds;Parent=MsG0280008659.01.T01
Chr2 three_prime_UTR 36006738 36006883 36006738 ID=MsG0280008659.01.T01:three_prime_utr;Parent=MsG0280008659.01.T01
Gene Sequence

>MsG0280008659.01.T01

ATGGCTTCAAGAAATGGTGAAGAGAAAGTTGAACGTGGCTTCAATGATGTTCATGAAAGAAAAACAAAGAATGATTGGATTCTTTTATCTCTTAGGGTGGTTGCATTTTTTGCCACTGCAGCTGCAACACTTGTGATGGCACTCAACAAACAGACCAAAAGTTTTATTGTTGGCACCGTTGGTAACACCCCAATAACTGCTACTATTTCTGCCAAATTTAATCAAACTCCAGCTTTTGTGTTCTTTGTGATAGCCAATGGAAATGCTAGTCTCCATAACTTGGTGATGATTGCATTGGATATTTTAGGACCCCAATATGATTACAAAGGACTTCGCCTTGCATTTATTGCAATTTTAGACATGTTAACTATGGCTCTGGCATCAGCTGGAGATGGTGCAGCAACATTCATGTCAGCATTGGGAAGAAATGGTAATTCACATGCAAGATGGGATAAGATTTGTGACAAATTTGAAAGCTATTGCAATAGAGGTGGTGGTGCCCTTACTGCTTCTTTCATTGGTTTCATTCTTCTCTTGATTATCACTGTGATGTCCATTGCCAAGCTTCTCAAGCCTAATCGCATTAACCATGCTCCTAGTCCTTGA

Protein sequence

>MsG0280008659.01.T01

MASRNGEEKVERGFNDVHERKTKNDWILLSLRVVAFFATAAATLVMALNKQTKSFIVGTVGNTPITATISAKFNQTPAFVFFVIANGNASLHNLVMIALDILGPQYDYKGLRLAFIAILDMLTMALASAGDGAATFMSALGRNGNSHARWDKICDKFESYCNRGGGALTASFIGFILLLIITVMSIAKLLKPNRINHAPSP*