Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001802.01.T01 | RHN78606.1 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 2.66E-63 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001802.01.T01 | A0A396JK39 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 1.27e-63 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049042.01 | MsG0180001802.01 | 0.834699 | 2.577633e-56 | 3.082403e-53 |
| MsG0180001079.01 | MsG0180001802.01 | 0.820882 | 5.423253e-53 | 4.344561e-50 |
| MsG0180001801.01 | MsG0180001802.01 | 0.990976 | 2.918180e-185 | 2.110488e-178 |
| MsG0180001802.01 | MsG0180001803.01 | 0.931994 | 1.654919e-94 | 1.081996e-89 |
| MsG0180001802.01 | MsG0180002935.01 | 0.824463 | 7.962832e-54 | 7.057752e-51 |
| MsG0180001802.01 | MsG0180003955.01 | 0.806469 | 8.104308e-50 | 4.402314e-47 |
| MsG0180001802.01 | MsG0180004491.01 | 0.820323 | 7.287149e-53 | 5.747378e-50 |
| MsG0180001802.01 | MsG0180005720.01 | 0.825601 | 4.288356e-54 | 3.927091e-51 |
| MsG0180001802.01 | MsG0180005817.01 | -0.846130 | 2.635520e-59 | 4.490878e-56 |
| MsG0180001802.01 | MsG0280006485.01 | 0.880740 | 4.278416e-70 | 2.440518e-66 |
| MsG0180001802.01 | MsG0280006932.01 | 0.819491 | 1.129636e-52 | 8.705864e-50 |
| MsG0180001802.01 | MsG0280007759.01 | 0.812393 | 4.337558e-51 | 2.754664e-48 |
| MsG0180001802.01 | MsG0280007760.01 | 0.811981 | 5.333977e-51 | 3.349688e-48 |
| MsG0180001802.01 | MsG0280008659.01 | 0.838970 | 2.094940e-57 | 2.851726e-54 |
| MsG0180001802.01 | MsG0280009416.01 | 0.849090 | 4.041181e-60 | 7.575598e-57 |
| MsG0180001802.01 | MsG0280009489.01 | 0.800805 | 1.213332e-48 | 5.702313e-46 |
| MsG0180001802.01 | MsG0280009656.01 | 0.831920 | 1.269819e-55 | 1.397939e-52 |
| MsG0180001802.01 | MsG0280010296.01 | 0.831133 | 1.983966e-55 | 2.133518e-52 |
| MsG0180001802.01 | MsG0280010410.01 | 0.807908 | 4.017140e-50 | 2.264895e-47 |
| MsG0180001802.01 | MsG0280010463.01 | 0.802827 | 4.662581e-49 | 2.306953e-46 |
| MsG0180001802.01 | MsG0280010502.01 | 0.829456 | 5.097299e-55 | 5.219556e-52 |
| MsG0180001802.01 | MsG0280011299.01 | -0.803181 | 3.941326e-49 | 1.967553e-46 |
| MsG0180001802.01 | MsG0380011635.01 | 0.803984 | 2.685572e-49 | 1.368694e-46 |
| MsG0180001802.01 | MsG0380011638.01 | 0.823671 | 1.221650e-53 | 1.058942e-50 |
| MsG0180001802.01 | MsG0380011974.01 | 0.804064 | 2.584329e-49 | 1.319817e-46 |
| MsG0180001802.01 | MsG0380013272.01 | 0.803276 | 3.765291e-49 | 1.884281e-46 |
| MsG0180001802.01 | MsG0380013409.01 | 0.805057 | 1.604012e-49 | 8.403566e-47 |
| MsG0180001802.01 | MsG0380015115.01 | 0.818132 | 2.298936e-52 | 1.706374e-49 |
| MsG0180001802.01 | MsG0380016476.01 | 0.806772 | 6.993346e-50 | 3.828398e-47 |
| MsG0180001802.01 | MsG0380017582.01 | 0.858671 | 7.018577e-63 | 1.805360e-59 |
| MsG0180001802.01 | MsG0380017590.01 | 0.825346 | 4.927746e-54 | 4.479813e-51 |
| MsG0180001802.01 | MsG0380017690.01 | 0.806977 | 6.330097e-50 | 3.483707e-47 |
| MsG0180001802.01 | MsG0480018440.01 | 0.826286 | 2.948401e-54 | 2.753151e-51 |
| MsG0180001802.01 | MsG0480018965.01 | 0.807733 | 4.375510e-50 | 2.455917e-47 |
| MsG0180001802.01 | MsG0480019923.01 | 0.822495 | 2.297255e-53 | 1.925954e-50 |
| MsG0180001802.01 | MsG0480019954.01 | 0.832420 | 9.550893e-56 | 1.066956e-52 |
| MsG0180001802.01 | MsG0480020814.01 | 0.813250 | 2.814877e-51 | 1.828740e-48 |
| MsG0180001802.01 | MsG0480020958.01 | 0.805201 | 1.496316e-49 | 7.868397e-47 |
| MsG0180001802.01 | MsG0480021553.01 | 0.808286 | 3.336435e-50 | 1.900158e-47 |
| MsG0180001802.01 | MsG0480021912.01 | -0.831928 | 1.263559e-55 | 1.391410e-52 |
| MsG0180001802.01 | MsG0480022735.01 | 0.817859 | 2.649784e-52 | 1.952149e-49 |
| MsG0180001802.01 | MsG0580025117.01 | 0.841751 | 3.930202e-58 | 5.831055e-55 |
| MsG0180001802.01 | MsG0580027036.01 | 0.820222 | 7.686068e-53 | 6.045292e-50 |
| MsG0180001802.01 | MsG0580028023.01 | 0.826148 | 3.179703e-54 | 2.957473e-51 |
| MsG0180001802.01 | MsG0680035841.01 | 0.855408 | 6.438654e-62 | 1.485122e-58 |
| MsG0180001802.01 | MsG0680035842.01 | 0.823592 | 1.275153e-53 | 1.102729e-50 |
| MsG0180001802.01 | MsG0780037751.01 | 0.851777 | 7.114534e-61 | 1.455653e-57 |
| MsG0180001802.01 | MsG0780038857.01 | 0.806053 | 9.915535e-50 | 5.329370e-47 |
| MsG0180001802.01 | MsG0780039190.01 | 0.815797 | 7.688672e-52 | 5.353715e-49 |
| MsG0180001802.01 | MsG0780040480.01 | 0.828087 | 1.092452e-54 | 1.074847e-51 |
| MsG0180001802.01 | MsG0780040553.01 | 0.805306 | 1.422865e-49 | 7.502038e-47 |
| MsG0180001802.01 | MsG0780040878.01 | 0.808000 | 3.839769e-50 | 2.170331e-47 |
| MsG0180001802.01 | MsG0780040977.01 | 0.822956 | 1.795157e-53 | 1.524805e-50 |
| MsG0180001802.01 | MsG0780041079.01 | 0.806418 | 8.307290e-50 | 4.506551e-47 |
| MsG0180001802.01 | MsG0880043261.01 | 0.813355 | 2.668986e-51 | 1.739094e-48 |
| MsG0180001802.01 | MsG0880043364.01 | 0.800754 | 1.242762e-48 | 5.833013e-46 |
| MsG0180001802.01 | MsG0880043447.01 | 0.811029 | 8.589037e-51 | 5.259892e-48 |
| MsG0180001802.01 | MsG0880044855.01 | 0.836225 | 1.060131e-56 | 1.327677e-53 |
| MsG0180001802.01 | MsG0880045804.01 | 0.826214 | 3.066550e-54 | 2.857659e-51 |
| MsG0180001802.01 | MsG0880046463.01 | 0.852925 | 3.350955e-61 | 7.114970e-58 |
| MsG0180001802.01 | MsG0880046838.01 | -0.815950 | 7.108896e-52 | 4.970347e-49 |
| MsG0180001802.01 | MsG0880047493.01 | 0.807258 | 5.518504e-50 | 3.059411e-47 |
| MsG0180001802.01 | MsG0880047714.01 | 0.818724 | 1.688263e-52 | 1.273723e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001802.01.T01 | MTR_1g043320 | 96.078 | 102 | 4 | 0 | 1 | 102 | 1 | 102 | 4.79e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGTGGCCAGTGTTTCTT+AGG | 0.235134 | 1:-27227492 | None:intergenic |
| ACTTCTCTAGCAGATCTTTC+AGG | 0.273797 | 1:+27227426 | MsG0180001802.01.T01:CDS |
| AAGACACAGTGAGATGTATT+TGG | 0.277898 | 1:-27227376 | None:intergenic |
| GGGATTGTTTGGTCCACTTT+TGG | 0.317502 | 1:-27227555 | None:intergenic |
| GTAGGAGGCCAGTGTTTCTT+TGG | 0.327069 | 1:-27227510 | None:intergenic |
| TGGAAGAAGCATCCTCCTTC+AGG | 0.366754 | 1:+27227648 | MsG0180001802.01.T01:CDS |
| ATCTCACTGTGTCTTCTACT+AGG | 0.408144 | 1:+27227384 | MsG0180001802.01.T01:CDS |
| AGACACAGTGAGATGTATTT+GGG | 0.409412 | 1:-27227375 | None:intergenic |
| CTTCTCTAGCAGATCTTTCA+GGG | 0.458151 | 1:+27227427 | MsG0180001802.01.T01:CDS |
| GGAGGCCAGTGTTTCTTTGG+TGG | 0.468858 | 1:-27227507 | None:intergenic |
| CTTGTGACTATCCTCAACTA+TGG | 0.473523 | 1:-27227607 | None:intergenic |
| ACAAAATGATCTTCCAAAAG+TGG | 0.476864 | 1:+27227542 | MsG0180001802.01.T01:CDS |
| GAAGGAGGATGCTTCTTCCA+TGG | 0.522552 | 1:-27227645 | None:intergenic |
| TCTCACTGTGTCTTCTACTA+GGG | 0.525074 | 1:+27227385 | MsG0180001802.01.T01:CDS |
| GACTATCCTCAACTATGGTA+TGG | 0.539163 | 1:-27227602 | None:intergenic |
| CCTCTGTGAGTGTTAATGCT+TGG | 0.552898 | 1:+27227457 | MsG0180001802.01.T01:CDS |
| ATTAATCAATTTCCTGAAGG+AGG | 0.566620 | 1:-27227660 | None:intergenic |
| AGCATCACCACAAGCGTCCA+TGG | 0.568001 | 1:+27227628 | MsG0180001802.01.T01:CDS |
| ATGATTAATCAATTTCCTGA+AGG | 0.570240 | 1:-27227663 | None:intergenic |
| ACTGGCCACCAAAGAAACAC+TGG | 0.593745 | 1:+27227502 | MsG0180001802.01.T01:CDS |
| GCTTCTTCCATGGACGCTTG+TGG | 0.612657 | 1:-27227635 | None:intergenic |
| ATAAGCAACCTAAGAAACAC+TGG | 0.614646 | 1:+27227484 | MsG0180001802.01.T01:CDS |
| AGAAGACCATACCATAGTTG+AGG | 0.630682 | 1:+27227596 | MsG0180001802.01.T01:CDS |
| TGAAAGATCTGCTAGAGAAG+TGG | 0.631719 | 1:-27227424 | None:intergenic |
| AAGATCTGCTAGAGAAGTGG+TGG | 0.634386 | 1:-27227421 | None:intergenic |
| TTCTCTAGCAGATCTTTCAG+GGG | 0.636352 | 1:+27227428 | MsG0180001802.01.T01:CDS |
| CCAAGCATTAACACTCACAG+AGG | 0.666199 | 1:-27227457 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAATGATCTTCCAAAAG+TGG | + | Chr1:27227542-27227561 | MsG0180001802.01.T01:CDS | 30.0% | |
| ! | AAGATCATTTTGTGTAGTTG+TGG | - | Chr1:27227535-27227554 | None:intergenic | 30.0% |
| AAGACACAGTGAGATGTATT+TGG | - | Chr1:27227379-27227398 | None:intergenic | 35.0% | |
| AGACACAGTGAGATGTATTT+GGG | - | Chr1:27227378-27227397 | None:intergenic | 35.0% | |
| ATAAGCAACCTAAGAAACAC+TGG | + | Chr1:27227484-27227503 | MsG0180001802.01.T01:CDS | 35.0% | |
| ! | TCATTTTGTGTAGTTGTGGT+AGG | - | Chr1:27227531-27227550 | None:intergenic | 35.0% |
| !!! | CTTGTGCTTGTTTGTTTTGT+GGG | - | Chr1:27227578-27227597 | None:intergenic | 35.0% |
| !!! | GTTTGTTTTGTGGGATTGTT+TGG | - | Chr1:27227569-27227588 | None:intergenic | 35.0% |
| !!! | TCTTGTGCTTGTTTGTTTTG+TGG | - | Chr1:27227579-27227598 | None:intergenic | 35.0% |
| ACTTCTCTAGCAGATCTTTC+AGG | + | Chr1:27227426-27227445 | MsG0180001802.01.T01:CDS | 40.0% | |
| AGAAGACCATACCATAGTTG+AGG | + | Chr1:27227596-27227615 | MsG0180001802.01.T01:CDS | 40.0% | |
| ATCTCACTGTGTCTTCTACT+AGG | + | Chr1:27227384-27227403 | MsG0180001802.01.T01:CDS | 40.0% | |
| CTTCTCTAGCAGATCTTTCA+GGG | + | Chr1:27227427-27227446 | MsG0180001802.01.T01:CDS | 40.0% | |
| CTTGTGACTATCCTCAACTA+TGG | - | Chr1:27227610-27227629 | None:intergenic | 40.0% | |
| GACTATCCTCAACTATGGTA+TGG | - | Chr1:27227605-27227624 | None:intergenic | 40.0% | |
| TCTCACTGTGTCTTCTACTA+GGG | + | Chr1:27227385-27227404 | MsG0180001802.01.T01:CDS | 40.0% | |
| TGAAAGATCTGCTAGAGAAG+TGG | - | Chr1:27227427-27227446 | None:intergenic | 40.0% | |
| TTCTCTAGCAGATCTTTCAG+GGG | + | Chr1:27227428-27227447 | MsG0180001802.01.T01:CDS | 40.0% | |
| ! | TTTTGTGTAGTTGTGGTAGG+AGG | - | Chr1:27227528-27227547 | None:intergenic | 40.0% |
| AAGATCTGCTAGAGAAGTGG+TGG | - | Chr1:27227424-27227443 | None:intergenic | 45.0% | |
| CCAAGCATTAACACTCACAG+AGG | - | Chr1:27227460-27227479 | None:intergenic | 45.0% | |
| CCTCTGTGAGTGTTAATGCT+TGG | + | Chr1:27227457-27227476 | MsG0180001802.01.T01:CDS | 45.0% | |
| ! | GGGATTGTTTGGTCCACTTT+TGG | - | Chr1:27227558-27227577 | None:intergenic | 45.0% |
| ! | TTTGGTGGCCAGTGTTTCTT+AGG | - | Chr1:27227495-27227514 | None:intergenic | 45.0% |
| ACTGGCCACCAAAGAAACAC+TGG | + | Chr1:27227502-27227521 | MsG0180001802.01.T01:CDS | 50.0% | |
| GAAGGAGGATGCTTCTTCCA+TGG | - | Chr1:27227648-27227667 | None:intergenic | 50.0% | |
| GTAGGAGGCCAGTGTTTCTT+TGG | - | Chr1:27227513-27227532 | None:intergenic | 50.0% | |
| !! | TGGAAGAAGCATCCTCCTTC+AGG | + | Chr1:27227648-27227667 | MsG0180001802.01.T01:CDS | 50.0% |
| AGCATCACCACAAGCGTCCA+TGG | + | Chr1:27227628-27227647 | MsG0180001802.01.T01:CDS | 55.0% | |
| GCTTCTTCCATGGACGCTTG+TGG | - | Chr1:27227638-27227657 | None:intergenic | 55.0% | |
| GGAGGCCAGTGTTTCTTTGG+TGG | - | Chr1:27227510-27227529 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 27227369 | 27227677 | 27227369 | ID=MsG0180001802.01;Name=MsG0180001802.01 |
| Chr1 | mRNA | 27227369 | 27227677 | 27227369 | ID=MsG0180001802.01.T01;Parent=MsG0180001802.01;Name=MsG0180001802.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|102 |
| Chr1 | exon | 27227369 | 27227677 | 27227369 | ID=MsG0180001802.01.T01:exon:34865;Parent=MsG0180001802.01.T01 |
| Chr1 | CDS | 27227369 | 27227677 | 27227369 | ID=MsG0180001802.01.T01:cds;Parent=MsG0180001802.01.T01 |
| Gene Sequence |
| Protein sequence |
