AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002364.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002364.01.T01 MTR_1g006590 95.200 125 6 0 12 136 123 247 8.30e-84 246
MsG0180002364.01.T01 MTR_1g006490 89.600 125 13 0 12 136 123 247 1.24e-77 230
MsG0180002364.01.T01 MTR_2g101370 56.296 135 55 1 2 132 114 248 2.81e-48 155
MsG0180002364.01.T01 MTR_8g013680 57.037 135 54 1 2 132 113 247 3.43e-48 155
MsG0180002364.01.T01 MTR_5g012810 55.645 124 54 1 10 133 124 246 1.51e-44 146
MsG0180002364.01.T01 MTR_4g063090 57.031 128 51 1 2 125 115 242 2.70e-44 145
MsG0180002364.01.T01 MTR_7g103030 59.167 120 49 0 10 129 126 245 1.50e-40 135
MsG0180002364.01.T01 MTR_1g115405 51.880 133 59 2 2 129 115 247 4.10e-40 135
MsG0180002364.01.T01 MTR_7g023350 53.383 133 57 2 2 129 115 247 4.38e-36 124
MsG0180002364.01.T01 MTR_6g069040 48.333 120 58 1 18 133 135 254 1.03e-35 123
MsG0180002364.01.T01 MTR_1g077540 47.500 120 56 1 21 133 138 257 3.32e-34 120
MsG0180002364.01.T01 MTR_2g060360 45.161 93 50 1 29 121 144 235 5.92e-23 90.5
MsG0180002364.01.T01 MTR_5g063930 41.441 111 60 3 26 136 155 260 2.44e-19 81.3
MsG0180002364.01.T01 MTR_2g094270 34.615 130 76 4 8 130 153 280 6.51e-17 75.1
MsG0180002364.01.T01 MTR_7g101190 39.130 115 61 4 16 123 159 271 1.95e-16 73.9
MsG0180002364.01.T01 MTR_3g008920 39.048 105 60 3 17 121 134 234 1.82e-15 70.9
MsG0180002364.01.T01 MTR_5g079570 35.780 109 67 2 26 134 156 261 2.17e-15 70.9
MsG0180002364.01.T01 MTR_4g059390 38.318 107 57 4 31 130 174 278 3.90e-15 70.5
MsG0180002364.01.T01 MTR_1g095070 37.500 120 66 4 18 130 95 212 1.27e-14 68.2
MsG0180002364.01.T01 MTR_2g017620 35.714 112 68 2 26 136 74 182 2.28e-14 66.6
MsG0180002364.01.T01 MTR_4g084880 39.423 104 59 2 26 129 154 253 4.82e-14 67.0
MsG0180002364.01.T01 MTR_2g017620 35.714 112 68 2 26 136 141 249 5.51e-14 67.0
MsG0180002364.01.T01 MTR_2g017620 35.714 112 68 2 26 136 155 263 7.27e-14 66.6
MsG0180002364.01.T01 MTR_2g017660 38.462 104 58 4 26 128 156 254 7.97e-14 66.6
MsG0180002364.01.T01 MTR_3g118010 36.449 107 59 4 31 130 166 270 1.07e-13 66.2
MsG0180002364.01.T01 MTR_4g415300 37.273 110 61 3 16 118 170 278 1.33e-13 66.2
MsG0180002364.01.T01 MTR_1g070385 37.000 100 58 3 23 121 175 270 1.35e-13 66.6
MsG0180002364.01.T01 MTR_2g017590 35.714 112 68 2 26 136 153 261 6.10e-13 64.3
MsG0180002364.01.T01 MTR_2g104940 40.000 95 52 3 17 110 188 278 9.34e-13 63.9
MsG0180002364.01.T01 MTR_1g097840 43.333 90 46 3 26 114 190 275 1.16e-12 63.5
MsG0180002364.01.T01 MTR_8g087720 35.577 104 63 2 26 129 155 254 1.41e-12 63.2
MsG0180002364.01.T01 MTR_4g006630 38.372 86 49 2 26 110 104 186 1.77e-12 62.4
MsG0180002364.01.T01 MTR_4g006730 38.372 86 49 2 26 110 104 186 1.81e-12 62.4
MsG0180002364.01.T01 MTR_5g010150 36.283 113 58 4 13 118 170 275 2.19e-12 62.8
MsG0180002364.01.T01 MTR_8g098375 36.441 118 60 5 13 123 170 279 4.19e-12 62.0
MsG0180002364.01.T01 MTR_7g010650 35.870 92 57 1 26 117 153 242 4.39e-12 62.0
MsG0180002364.01.T01 MTR_4g006730 38.372 86 49 2 26 110 191 273 4.51e-12 62.0
MsG0180002364.01.T01 MTR_8g087710 32.743 113 71 3 17 129 148 255 9.53e-12 60.8
MsG0180002364.01.T01 MTR_3g070210 36.752 117 59 5 14 123 174 282 9.83e-12 60.8
MsG0180002364.01.T01 MTR_5g082070 36.607 112 57 4 13 117 173 277 1.39e-11 60.5
MsG0180002364.01.T01 MTR_8g468730 33.333 93 43 2 29 121 8 81 4.57e-11 56.2
MsG0180002364.01.T01 MTR_2g017610 38.372 86 49 2 26 110 155 237 9.48e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002364.01.T01 AT2G25810 81.967 122 22 0 13 134 124 245 2.12e-69 209
MsG0180002364.01.T01 AT2G36830 56.818 132 53 1 2 129 115 246 1.12e-46 151
MsG0180002364.01.T01 AT3G16240 58.974 117 47 1 14 129 129 245 1.63e-45 148
MsG0180002364.01.T01 AT4G17340 52.555 137 60 2 2 134 113 248 4.15e-45 147
MsG0180002364.01.T01 AT5G47450 55.118 127 53 1 2 124 113 239 7.14e-45 147
MsG0180002364.01.T01 AT3G26520 60.000 115 46 0 11 125 129 243 4.12e-43 142
MsG0180002364.01.T01 AT1G73190 53.600 125 54 2 2 122 123 247 1.77e-39 134
MsG0180002364.01.T01 AT4G01470 50.365 137 63 2 2 133 115 251 4.46e-38 129
MsG0180002364.01.T01 AT1G17810 49.194 124 55 1 18 133 143 266 1.23e-37 129
MsG0180002364.01.T01 AT3G47440 44.248 113 62 1 10 122 129 240 7.84e-27 100
MsG0180002364.01.T01 AT1G52180 47.727 88 46 0 10 97 35 122 1.99e-24 91.3
MsG0180002364.01.T01 AT2G37170 36.000 125 71 4 8 125 192 314 8.06e-17 75.5
MsG0180002364.01.T01 AT2G37170 36.000 125 71 4 8 125 149 271 8.71e-17 74.7
MsG0180002364.01.T01 AT5G60660 37.705 122 67 4 16 130 159 278 1.15e-16 74.7
MsG0180002364.01.T01 AT5G37820 41.346 104 57 3 26 129 160 259 1.37e-16 74.3
MsG0180002364.01.T01 AT2G39010 39.130 115 61 4 16 123 158 270 1.44e-16 74.3
MsG0180002364.01.T01 AT5G37820 41.346 104 57 3 26 129 196 295 1.95e-16 74.3
MsG0180002364.01.T01 AT5G37810 41.346 104 57 2 26 129 160 259 2.23e-16 73.6
MsG0180002364.01.T01 AT3G53420 37.607 117 64 4 16 125 159 273 2.77e-16 73.6
MsG0180002364.01.T01 AT3G53420 37.607 117 64 4 16 125 159 273 2.77e-16 73.6
MsG0180002364.01.T01 AT2G37180 38.261 115 62 4 18 125 159 271 1.20e-15 71.6
MsG0180002364.01.T01 AT4G35100 36.364 132 68 6 13 130 142 271 1.71e-15 71.2
MsG0180002364.01.T01 AT4G35100 36.364 132 68 6 13 130 142 271 1.71e-15 71.2
MsG0180002364.01.T01 AT2G16850 33.858 127 75 4 16 135 150 274 9.88e-15 69.3
MsG0180002364.01.T01 AT4G10380 39.604 101 57 2 26 125 194 291 4.03e-14 67.8
MsG0180002364.01.T01 AT1G31885 40.698 86 49 1 25 110 112 195 4.87e-14 67.0
MsG0180002364.01.T01 AT1G31885 40.698 86 49 1 25 110 166 249 5.44e-14 67.4
MsG0180002364.01.T01 AT3G54820 36.283 113 63 4 18 123 160 270 1.10e-13 66.2
MsG0180002364.01.T01 AT4G00430 34.286 140 69 6 1 118 137 275 3.34e-13 65.1
MsG0180002364.01.T01 AT1G80760 38.889 90 51 2 22 110 192 278 7.00e-13 64.3
MsG0180002364.01.T01 AT4G18910 36.207 116 67 4 15 129 166 275 1.15e-12 63.5
MsG0180002364.01.T01 AT1G01620 36.066 122 63 5 11 118 82 202 1.96e-12 62.0
MsG0180002364.01.T01 AT1G01620 36.066 122 63 5 11 118 154 274 2.34e-12 62.8
MsG0180002364.01.T01 AT4G23400 37.273 110 55 4 16 118 173 275 3.35e-12 62.4
MsG0180002364.01.T01 AT2G45960 36.697 109 61 3 16 117 166 273 4.19e-12 62.0
MsG0180002364.01.T01 AT3G61430 36.697 109 61 3 16 117 166 273 4.63e-12 62.0
MsG0180002364.01.T01 AT3G61430 36.697 109 61 3 16 117 166 273 4.63e-12 62.0
MsG0180002364.01.T01 AT4G19030 34.286 105 63 3 26 129 179 278 3.52e-11 59.7
MsG0180002364.01.T01 AT3G06100 30.693 101 69 1 19 119 74 173 5.21e-11 58.2

Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGAAAGGAGGACTTTC+TGG 0.139053 1:-37417982 MsG0180002364.01.T01:CDS
ACAACAAATCCAACCAAAAT+TGG 0.151579 1:+37417952 None:intergenic
CTCATTGGTGGTGGGCTTGC+TGG 0.168087 1:-37417808 MsG0180002364.01.T01:CDS
TTCTGGACTTGGCCCAATTT+TGG 0.189335 1:-37417965 MsG0180002364.01.T01:CDS
CCAGAAAGTCCTCCTTTCTT+TGG 0.192856 1:+37417982 None:intergenic
CTGCATTGGTTAGTGGAAAT+TGG 0.223669 1:-37417861 MsG0180002364.01.T01:CDS
ATGAATCCAGCAAGATCTTT+TGG 0.260996 1:-37417886 MsG0180002364.01.T01:CDS
GGGCTAACATCTTGGCTGGC+GGG 0.363981 1:-37417927 MsG0180002364.01.T01:CDS
GGTGTACTCAAGGAGTAGTT+TGG 0.378665 1:-37418059 MsG0180002364.01.T01:CDS
GTTCACTGTGTATGCAACTA+TGG 0.382620 1:-37418010 MsG0180002364.01.T01:CDS
AAAAGATCTTGCTGGATTCA+TGG 0.384219 1:+37417888 None:intergenic
TTGGCTGGCGGGGCTTTCGC+AGG 0.386405 1:-37417916 MsG0180002364.01.T01:CDS
TTTGGACCTGCATTGGTTAG+TGG 0.401163 1:-37417868 MsG0180002364.01.T01:CDS
TGTTGTAGGGGCTAACATCT+TGG 0.405323 1:-37417935 MsG0180002364.01.T01:CDS
TTGGGCCTCTCATTGGTGGT+GGG 0.408100 1:-37417816 MsG0180002364.01.T01:CDS
TGGGTTGGGCCTCTCATTGG+TGG 0.430148 1:-37417820 MsG0180002364.01.T01:CDS
GTTGGGCCTCTCATTGGTGG+TGG 0.433600 1:-37417817 MsG0180002364.01.T01:CDS
CAACAAATCCAACCAAAATT+GGG 0.439747 1:+37417953 None:intergenic
AAAGGAGGACTTTCTGGACT+TGG 0.441144 1:-37417976 MsG0180002364.01.T01:CDS
GCAGGTCCAAAAGATCTTGC+TGG 0.456957 1:+37417880 None:intergenic
TGGAAATTGGACTGACCAAT+GGG 0.457985 1:-37417848 MsG0180002364.01.T01:CDS
GGCTAACATCTTGGCTGGCG+GGG 0.461965 1:-37417926 MsG0180002364.01.T01:CDS
GTGTACTCAAGGAGTAGTTT+GGG 0.480313 1:-37418058 MsG0180002364.01.T01:CDS
CATACACTTAAAAGTGGAGT+AGG 0.484564 1:-37418081 MsG0180002364.01.T01:CDS
CCAGTTCATACACTTAAAAG+TGG 0.496805 1:-37418087 MsG0180002364.01.T01:CDS
GTGGAAATTGGACTGACCAA+TGG 0.502211 1:-37417849 MsG0180002364.01.T01:CDS
GGGGCTAACATCTTGGCTGG+CGG 0.514242 1:-37417928 MsG0180002364.01.T01:CDS
GTAGGGGCTAACATCTTGGC+TGG 0.532312 1:-37417931 MsG0180002364.01.T01:CDS
CAATTTCCACTAACCAATGC+AGG 0.555256 1:+37417862 None:intergenic
ACTATGGTTGATCCAAAGAA+AGG 0.565384 1:-37417994 MsG0180002364.01.T01:CDS
AACTCATGTTCCTCTCCTTG+TGG 0.565628 1:-37417755 MsG0180002364.01.T01:CDS
TTTGGTTGGATTTGTTGTAG+GGG 0.576606 1:-37417947 MsG0180002364.01.T01:CDS
GCAAGCCCACCACCAATGAG+AGG 0.582625 1:+37417811 None:intergenic
CTTTCTTCATCCACAAGGAG+AGG 0.595545 1:+37417745 None:intergenic
AGTGGAGTAGGGTGTACTCA+AGG 0.614383 1:-37418069 MsG0180002364.01.T01:CDS
AGTAGCTTTCTTCATCCACA+AGG 0.615821 1:+37417740 None:intergenic
ATACACTTAAAAGTGGAGTA+GGG 0.631270 1:-37418080 MsG0180002364.01.T01:CDS
ATGGTTGATCCAAAGAAAGG+AGG 0.657733 1:-37417991 MsG0180002364.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAACAAATCCAACCAAAAT+TGG + Chr1:37417915-37417934 None:intergenic 30.0%
ATACACTTAAAAGTGGAGTA+GGG - Chr1:37417784-37417803 MsG0180002364.01.T01:CDS 30.0%
CAACAAATCCAACCAAAATT+GGG + Chr1:37417914-37417933 None:intergenic 30.0%
!!! ATTTTGGTTGGATTTGTTGT+AGG - Chr1:37417915-37417934 MsG0180002364.01.T01:CDS 30.0%
!!! TTTTGGTTGGATTTGTTGTA+GGG - Chr1:37417916-37417935 MsG0180002364.01.T01:CDS 30.0%
AAAAGATCTTGCTGGATTCA+TGG + Chr1:37417979-37417998 None:intergenic 35.0%
ACTATGGTTGATCCAAAGAA+AGG - Chr1:37417870-37417889 MsG0180002364.01.T01:CDS 35.0%
CATACACTTAAAAGTGGAGT+AGG - Chr1:37417783-37417802 MsG0180002364.01.T01:CDS 35.0%
CCAGTTCATACACTTAAAAG+TGG - Chr1:37417777-37417796 MsG0180002364.01.T01:CDS 35.0%
! ATGAATCCAGCAAGATCTTT+TGG - Chr1:37417978-37417997 MsG0180002364.01.T01:CDS 35.0%
!! CCACTTTTAAGTGTATGAAC+TGG + Chr1:37417780-37417799 None:intergenic 35.0%
!! TTTGGTTGGATTTGTTGTAG+GGG - Chr1:37417917-37417936 MsG0180002364.01.T01:CDS 35.0%
AGTAGCTTTCTTCATCCACA+AGG + Chr1:37418127-37418146 None:intergenic 40.0%
ATGGTTGATCCAAAGAAAGG+AGG - Chr1:37417873-37417892 MsG0180002364.01.T01:CDS 40.0%
CAATTTCCACTAACCAATGC+AGG + Chr1:37418005-37418024 None:intergenic 40.0%
GTGTACTCAAGGAGTAGTTT+GGG - Chr1:37417806-37417825 MsG0180002364.01.T01:CDS 40.0%
GTTCACTGTGTATGCAACTA+TGG - Chr1:37417854-37417873 MsG0180002364.01.T01:CDS 40.0%
TGGAAATTGGACTGACCAAT+GGG - Chr1:37418016-37418035 MsG0180002364.01.T01:CDS 40.0%
! AAGATCTTTTGGACCTGCAT+TGG - Chr1:37417989-37418008 MsG0180002364.01.T01:CDS 40.0%
!! CTGCATTGGTTAGTGGAAAT+TGG - Chr1:37418003-37418022 MsG0180002364.01.T01:CDS 40.0%
!!! ACCAATGGGTTTTTTGGGTT+GGG - Chr1:37418030-37418049 MsG0180002364.01.T01:CDS 40.0%
!!! GACTGACCAATGGGTTTTTT+GGG - Chr1:37418025-37418044 MsG0180002364.01.T01:CDS 40.0%
AAAGGAGGACTTTCTGGACT+TGG - Chr1:37417888-37417907 MsG0180002364.01.T01:CDS 45.0%
AACTCATGTTCCTCTCCTTG+TGG - Chr1:37418109-37418128 MsG0180002364.01.T01:CDS 45.0%
CCAAAGAAAGGAGGACTTTC+TGG - Chr1:37417882-37417901 MsG0180002364.01.T01:CDS 45.0%
CCAGAAAGTCCTCCTTTCTT+TGG + Chr1:37417885-37417904 None:intergenic 45.0%
CTTTCTTCATCCACAAGGAG+AGG + Chr1:37418122-37418141 None:intergenic 45.0%
GGTGTACTCAAGGAGTAGTT+TGG - Chr1:37417805-37417824 MsG0180002364.01.T01:CDS 45.0%
GTGGAAATTGGACTGACCAA+TGG - Chr1:37418015-37418034 MsG0180002364.01.T01:CDS 45.0%
TGTTGTAGGGGCTAACATCT+TGG - Chr1:37417929-37417948 MsG0180002364.01.T01:CDS 45.0%
! TTCTGGACTTGGCCCAATTT+TGG - Chr1:37417899-37417918 MsG0180002364.01.T01:CDS 45.0%
!! TTTGGACCTGCATTGGTTAG+TGG - Chr1:37417996-37418015 MsG0180002364.01.T01:CDS 45.0%
!!! GACCAATGGGTTTTTTGGGT+TGG - Chr1:37418029-37418048 MsG0180002364.01.T01:CDS 45.0%
!!! GGACTGACCAATGGGTTTTT+TGG - Chr1:37418024-37418043 MsG0180002364.01.T01:CDS 45.0%
AGTGGAGTAGGGTGTACTCA+AGG - Chr1:37417795-37417814 MsG0180002364.01.T01:CDS 50.0%
GCAGGTCCAAAAGATCTTGC+TGG + Chr1:37417987-37418006 None:intergenic 50.0%
GCCCAACCCAAAAAACCCAT+TGG + Chr1:37418034-37418053 None:intergenic 50.0%
!! GGACTTGGCCCAATTTTGGT+TGG - Chr1:37417903-37417922 MsG0180002364.01.T01:CDS 50.0%
!!! TTTTGGGTTGGGCCTCTCAT+TGG - Chr1:37418041-37418060 MsG0180002364.01.T01:CDS 50.0%
GTAGGGGCTAACATCTTGGC+TGG - Chr1:37417933-37417952 MsG0180002364.01.T01:CDS 55.0%
! TTGGGCCTCTCATTGGTGGT+GGG - Chr1:37418048-37418067 MsG0180002364.01.T01:CDS 55.0%
GCAAGCCCACCACCAATGAG+AGG + Chr1:37418056-37418075 None:intergenic 60.0%
GGCTAACATCTTGGCTGGCG+GGG - Chr1:37417938-37417957 MsG0180002364.01.T01:CDS 60.0%
GGGCTAACATCTTGGCTGGC+GGG - Chr1:37417937-37417956 MsG0180002364.01.T01:CDS 60.0%
GGGGCTAACATCTTGGCTGG+CGG - Chr1:37417936-37417955 MsG0180002364.01.T01:CDS 60.0%
GTTGGGCCTCTCATTGGTGG+TGG - Chr1:37418047-37418066 MsG0180002364.01.T01:CDS 60.0%
! TGGGTTGGGCCTCTCATTGG+TGG - Chr1:37418044-37418063 MsG0180002364.01.T01:CDS 60.0%
!! CTCATTGGTGGTGGGCTTGC+TGG - Chr1:37418056-37418075 MsG0180002364.01.T01:CDS 60.0%
TTGGCTGGCGGGGCTTTCGC+AGG - Chr1:37417948-37417967 MsG0180002364.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 37417738 37418148 37417738 ID=MsG0180002364.01;Name=MsG0180002364.01
Chr1 mRNA 37417738 37418148 37417738 ID=MsG0180002364.01.T01;Parent=MsG0180002364.01;Name=MsG0180002364.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136
Chr1 exon 37417738 37418148 37417738 ID=MsG0180002364.01.T01:exon:419;Parent=MsG0180002364.01.T01
Chr1 CDS 37417738 37418148 37417738 ID=MsG0180002364.01.T01:cds;Parent=MsG0180002364.01.T01
Gene Sequence

>MsG0180002364.01.T01

ATGATTGTACATTGTATCTTGCATGATCAACAGACAACTCCAGTTCATACACTTAAAAGTGGAGTAGGGTGTACTCAAGGAGTAGTTTGGGAAATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACTATGGTTGATCCAAAGAAAGGAGGACTTTCTGGACTTGGCCCAATTTTGGTTGGATTTGTTGTAGGGGCTAACATCTTGGCTGGCGGGGCTTTCGCAGGTGCTTCCATGAATCCAGCAAGATCTTTTGGACCTGCATTGGTTAGTGGAAATTGGACTGACCAATGGGTTTTTTGGGTTGGGCCTCTCATTGGTGGTGGGCTTGCTGGTTTCATTTATGAGAATTTTCTTATCAATAGAACTCATGTTCCTCTCCTTGTGGATGAAGAAAGCTACTAA

Protein sequence

>MsG0180002364.01.T01

MIVHCILHDQQTTPVHTLKSGVGCTQGVVWEIVLTFSLLFTVYATMVDPKKGGLSGLGPILVGFVVGANILAGGAFAGASMNPARSFGPALVSGNWTDQWVFWVGPLIGGGLAGFIYENFLINRTHVPLLVDEESY*