AlfalfaGEDB | MsG0180002364.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002364.01

KEGG: K09873
GO: GO:0015267 GO:0016020 GO:0055085
PFAM: PF00230

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002364.01.T01	XP_003588378.2	95.2	125	6	0	12	136	123	247	6.85E-80	246

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002364.01.T01	O82316	81.967	122	22	0	13	134	124	245	2.08E-68	209

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002364.01.T01	G7IB42	95.200	125	6	0	12	136	123	247	3.27e-80	246

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048134.01	MsG0180002364.01	0.806881	6.632706e-50	3.641219e-47
MsG0180000287.01	MsG0180002364.01	0.821326	4.284298e-53	3.474758e-50
MsG0180000870.01	MsG0180002364.01	0.982736	7.266895e-156	2.176315e-149
MsG0180002323.01	MsG0180002364.01	0.870678	1.216920e-66	4.774606e-63
MsG0180002364.01	MsG0180003320.01	0.801280	9.699546e-49	4.613825e-46
MsG0180002364.01	MsG0280007922.01	0.829566	4.792920e-55	4.923510e-52
MsG0180002364.01	MsG0280011267.01	0.810274	1.250278e-50	7.503927e-48
MsG0180002364.01	MsG0380012889.01	0.804909	1.722263e-49	8.989645e-47
MsG0180002364.01	MsG0380012981.01	0.809071	2.268192e-50	1.318731e-47
MsG0180002364.01	MsG0380013285.01	0.801508	8.712859e-49	4.168300e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002364.01.T01	MTR_1g006590	95.200	125	6	0	12	136	123	247	8.30e-84	246
MsG0180002364.01.T01	MTR_1g006490	89.600	125	13	0	12	136	123	247	1.24e-77	230
MsG0180002364.01.T01	MTR_2g101370	56.296	135	55	1	2	132	114	248	2.81e-48	155
MsG0180002364.01.T01	MTR_8g013680	57.037	135	54	1	2	132	113	247	3.43e-48	155
MsG0180002364.01.T01	MTR_5g012810	55.645	124	54	1	10	133	124	246	1.51e-44	146
MsG0180002364.01.T01	MTR_4g063090	57.031	128	51	1	2	125	115	242	2.70e-44	145
MsG0180002364.01.T01	MTR_7g103030	59.167	120	49	0	10	129	126	245	1.50e-40	135
MsG0180002364.01.T01	MTR_1g115405	51.880	133	59	2	2	129	115	247	4.10e-40	135
MsG0180002364.01.T01	MTR_7g023350	53.383	133	57	2	2	129	115	247	4.38e-36	124
MsG0180002364.01.T01	MTR_6g069040	48.333	120	58	1	18	133	135	254	1.03e-35	123

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002364.01.T01	AT2G25810	81.967	122	22	0	13	134	124	245	2.12e-69	209
MsG0180002364.01.T01	AT2G36830	56.818	132	53	1	2	129	115	246	1.12e-46	151
MsG0180002364.01.T01	AT3G16240	58.974	117	47	1	14	129	129	245	1.63e-45	148
MsG0180002364.01.T01	AT4G17340	52.555	137	60	2	2	134	113	248	4.15e-45	147
MsG0180002364.01.T01	AT5G47450	55.118	127	53	1	2	124	113	239	7.14e-45	147
MsG0180002364.01.T01	AT3G26520	60.000	115	46	0	11	125	129	243	4.12e-43	142
MsG0180002364.01.T01	AT1G73190	53.600	125	54	2	2	122	123	247	1.77e-39	134
MsG0180002364.01.T01	AT4G01470	50.365	137	63	2	2	133	115	251	4.46e-38	129
MsG0180002364.01.T01	AT1G17810	49.194	124	55	1	18	133	143	266	1.23e-37	129
MsG0180002364.01.T01	AT3G47440	44.248	113	62	1	10	122	129	240	7.84e-27	100

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Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATCTTGCTGGATTCA+TGG	0.384219	1:+37417888	None:intergenic
AAAGGAGGACTTTCTGGACT+TGG	0.441144	1:-37417976	MsG0180002364.01.T01:CDS
AACTCATGTTCCTCTCCTTG+TGG	0.565628	1:-37417755	MsG0180002364.01.T01:CDS
ACAACAAATCCAACCAAAAT+TGG	0.151579	1:+37417952	None:intergenic
ACTATGGTTGATCCAAAGAA+AGG	0.565384	1:-37417994	MsG0180002364.01.T01:CDS
AGTAGCTTTCTTCATCCACA+AGG	0.615821	1:+37417740	None:intergenic
AGTGGAGTAGGGTGTACTCA+AGG	0.614383	1:-37418069	MsG0180002364.01.T01:CDS
ATACACTTAAAAGTGGAGTA+GGG	0.631270	1:-37418080	MsG0180002364.01.T01:CDS
ATGAATCCAGCAAGATCTTT+TGG	0.260996	1:-37417886	MsG0180002364.01.T01:CDS
ATGGTTGATCCAAAGAAAGG+AGG	0.657733	1:-37417991	MsG0180002364.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAACAAATCCAACCAAAAT+TGG	+	Chr1:37417915-37417934	None:intergenic	30.0%
ATACACTTAAAAGTGGAGTA+GGG	-	Chr1:37417784-37417803	MsG0180002364.01.T01:CDS	30.0%
CAACAAATCCAACCAAAATT+GGG	+	Chr1:37417914-37417933	None:intergenic	30.0%
AAAAGATCTTGCTGGATTCA+TGG	+	Chr1:37417979-37417998	None:intergenic	35.0%
ACTATGGTTGATCCAAAGAA+AGG	-	Chr1:37417870-37417889	MsG0180002364.01.T01:CDS	35.0%
CATACACTTAAAAGTGGAGT+AGG	-	Chr1:37417783-37417802	MsG0180002364.01.T01:CDS	35.0%
CCAGTTCATACACTTAAAAG+TGG	-	Chr1:37417777-37417796	MsG0180002364.01.T01:CDS	35.0%
AGTAGCTTTCTTCATCCACA+AGG	+	Chr1:37418127-37418146	None:intergenic	40.0%
ATGGTTGATCCAAAGAAAGG+AGG	-	Chr1:37417873-37417892	MsG0180002364.01.T01:CDS	40.0%
CAATTTCCACTAACCAATGC+AGG	+	Chr1:37418005-37418024	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	37417738	37418148	37417738	ID=MsG0180002364.01;Name=MsG0180002364.01
Chr1	mRNA	37417738	37418148	37417738	ID=MsG0180002364.01.T01;Parent=MsG0180002364.01;Name=MsG0180002364.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
Chr1	exon	37417738	37418148	37417738	ID=MsG0180002364.01.T01:exon:419;Parent=MsG0180002364.01.T01
Chr1	CDS	37417738	37418148	37417738	ID=MsG0180002364.01.T01:cds;Parent=MsG0180002364.01.T01

Gene Sequence

>MsG0180002364.01.T01

ATGATTGTACATTGTATCTTGCATGATCAACAGACAACTCCAGTTCATACACTTAAAAGTGGAGTAGGGTGTACTCAAGGAGTAGTTTGGGAAATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACTATGGTTGATCCAAAGAAAGGAGGACTTTCTGGACTTGGCCCAATTTTGGTTGGATTTGTTGTAGGGGCTAACATCTTGGCTGGCGGGGCTTTCGCAGGTGCTTCCATGAATCCAGCAAGATCTTTTGGACCTGCATTGGTTAGTGGAAATTGGACTGACCAATGGGTTTTTTGGGTTGGGCCTCTCATTGGTGGTGGGCTTGCTGGTTTCATTTATGAGAATTTTCTTATCAATAGAACTCATGTTCCTCTCCTTGTGGATGAAGAAAGCTACTAA

Protein sequence

>MsG0180002364.01.T01

MIVHCILHDQQTTPVHTLKSGVGCTQGVVWEIVLTFSLLFTVYATMVDPKKGGLSGLGPILVGFVVGANILAGGAFAGASMNPARSFGPALVSGNWTDQWVFWVGPLIGGGLAGFIYENFLINRTHVPLLVDEESY*