Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000287.01.T01 | XP_003589150.1 | 92.634 | 448 | 28 | 4 | 1 | 445 | 1 | 446 | 0 | 813 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000287.01.T01 | G7I2D2 | 92.634 | 448 | 28 | 4 | 1 | 445 | 1 | 446 | 0.0 | 813 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048889.01 | MsG0180000287.01 | 0.806271 | 8.920343e-50 | 4.821381e-47 |
| MsG0080049023.01 | MsG0180000287.01 | -0.803513 | 3.363619e-49 | 1.693613e-46 |
| MsG0080049066.01 | MsG0180000287.01 | -0.803651 | 3.148597e-49 | 1.590891e-46 |
| MsG0180000287.01 | MsG0180000870.01 | 0.821576 | 3.751905e-53 | 3.064111e-50 |
| MsG0180000287.01 | MsG0180001497.01 | 0.800500 | 1.400214e-48 | 6.529869e-46 |
| MsG0180000287.01 | MsG0180001513.01 | 0.817791 | 2.744866e-52 | 2.018461e-49 |
| MsG0180000287.01 | MsG0180001515.01 | 0.814259 | 1.687690e-51 | 1.126943e-48 |
| MsG0180000287.01 | MsG0180002364.01 | 0.821326 | 4.284298e-53 | 3.474758e-50 |
| MsG0180000287.01 | MsG0180002808.01 | 0.819524 | 1.109846e-52 | 8.561389e-50 |
| MsG0180000287.01 | MsG0180003375.01 | 0.817891 | 2.605943e-52 | 1.921468e-49 |
| MsG0180000287.01 | MsG0180003482.01 | 0.800811 | 1.209616e-48 | 5.685648e-46 |
| MsG0180000287.01 | MsG0180003484.01 | 0.805106 | 1.566912e-49 | 8.219351e-47 |
| MsG0180000287.01 | MsG0180004359.01 | 0.800916 | 1.151646e-48 | 5.427981e-46 |
| MsG0180000287.01 | MsG0180005353.01 | 0.812700 | 3.714666e-51 | 2.378256e-48 |
| MsG0180000287.01 | MsG0180005369.01 | -0.810851 | 9.384758e-51 | 5.719982e-48 |
| MsG0180000287.01 | MsG0180005417.01 | 0.819444 | 1.157441e-52 | 8.908633e-50 |
| MsG0180000287.01 | MsG0180005757.01 | 0.816146 | 6.428942e-52 | 4.519213e-49 |
| MsG0180000287.01 | MsG0280006316.01 | 0.804743 | 1.865816e-49 | 9.696987e-47 |
| MsG0180000287.01 | MsG0280006381.01 | -0.801594 | 8.366267e-49 | 4.010967e-46 |
| MsG0180000287.01 | MsG0280006575.01 | 0.827211 | 1.773431e-54 | 1.700814e-51 |
| MsG0180000287.01 | MsG0280008328.01 | 0.800139 | 1.657740e-48 | 7.660710e-46 |
| MsG0180000287.01 | MsG0280009742.01 | -0.812267 | 4.620461e-51 | 2.924387e-48 |
| MsG0180000287.01 | MsG0280010515.01 | 0.811321 | 7.421723e-51 | 4.579724e-48 |
| MsG0180000287.01 | MsG0280011267.01 | 0.836434 | 9.382161e-57 | 1.182241e-53 |
| MsG0180000287.01 | MsG0380011974.01 | 0.809637 | 1.715005e-50 | 1.012339e-47 |
| MsG0180000287.01 | MsG0380012889.01 | 0.827083 | 1.903028e-54 | 1.818297e-51 |
| MsG0180000287.01 | MsG0380012900.01 | 0.837441 | 5.188673e-57 | 6.740792e-54 |
| MsG0180000287.01 | MsG0380013272.01 | 0.803657 | 3.139939e-49 | 1.586744e-46 |
| MsG0180000287.01 | MsG0380013285.01 | 0.809204 | 2.124075e-50 | 1.239411e-47 |
| MsG0180000287.01 | MsG0380013415.01 | 0.817838 | 2.679301e-52 | 1.972757e-49 |
| MsG0180000287.01 | MsG0380013843.01 | 0.800927 | 1.145438e-48 | 5.400230e-46 |
| MsG0180000287.01 | MsG0380014410.01 | 0.800320 | 1.523305e-48 | 7.071882e-46 |
| MsG0180000287.01 | MsG0380014639.01 | -0.818293 | 2.114241e-52 | 1.576222e-49 |
| MsG0180000287.01 | MsG0380014642.01 | -0.824805 | 6.615150e-54 | 5.922296e-51 |
| MsG0180000287.01 | MsG0380014735.01 | -0.806964 | 6.368841e-50 | 3.503852e-47 |
| MsG0180000287.01 | MsG0380015080.01 | 0.802231 | 6.188097e-49 | 3.015275e-46 |
| MsG0180000287.01 | MsG0380016476.01 | 0.824011 | 1.017006e-53 | 8.898993e-51 |
| MsG0180000287.01 | MsG0380016935.01 | -0.805537 | 1.272499e-49 | 6.749168e-47 |
| MsG0180000287.01 | MsG0380017405.01 | -0.802555 | 5.307425e-49 | 2.607489e-46 |
| MsG0180000287.01 | MsG0480018439.01 | 0.804489 | 2.107943e-49 | 1.088248e-46 |
| MsG0180000287.01 | MsG0480018559.01 | 0.801900 | 7.239697e-49 | 3.498154e-46 |
| MsG0180000287.01 | MsG0480019666.01 | 0.821116 | 4.791414e-53 | 3.863421e-50 |
| MsG0180000287.01 | MsG0480020722.01 | 0.803479 | 3.418373e-49 | 1.719650e-46 |
| MsG0180000287.01 | MsG0480020814.01 | 0.812033 | 5.197595e-51 | 3.268494e-48 |
| MsG0180000287.01 | MsG0480021129.01 | 0.812261 | 4.634153e-51 | 2.932595e-48 |
| MsG0180000287.01 | MsG0480021133.01 | 0.817150 | 3.829143e-52 | 2.766658e-49 |
| MsG0180000287.01 | MsG0480021360.01 | 0.822346 | 2.488099e-53 | 2.077187e-50 |
| MsG0180000287.01 | MsG0480021573.01 | 0.830887 | 2.279976e-55 | 2.434144e-52 |
| MsG0180000287.01 | MsG0480022309.01 | 0.811654 | 6.283543e-51 | 3.911518e-48 |
| MsG0180000287.01 | MsG0480022310.01 | 0.815244 | 1.020985e-51 | 7.001414e-49 |
| MsG0180000287.01 | MsG0480022428.01 | 0.816382 | 5.690712e-52 | 4.026204e-49 |
| MsG0180000287.01 | MsG0480022833.01 | 0.810114 | 1.354119e-50 | 8.093481e-48 |
| MsG0180000287.01 | MsG0480023322.01 | 0.806944 | 6.431512e-50 | 3.536522e-47 |
| MsG0180000287.01 | MsG0580025023.01 | 0.853903 | 1.756823e-61 | 3.855062e-58 |
| MsG0180000287.01 | MsG0580025117.01 | 0.800786 | 1.223670e-48 | 5.748079e-46 |
| MsG0180000287.01 | MsG0580025961.01 | 0.834124 | 3.592675e-56 | 4.223318e-53 |
| MsG0180000287.01 | MsG0580026073.01 | -0.801405 | 9.143612e-49 | 4.363049e-46 |
| MsG0180000287.01 | MsG0580026148.01 | 0.826396 | 2.776333e-54 | 2.600593e-51 |
| MsG0180000287.01 | MsG0580026492.01 | 0.809425 | 1.903985e-50 | 1.117542e-47 |
| MsG0180000287.01 | MsG0580027276.01 | -0.809759 | 1.614240e-50 | 9.559510e-48 |
| MsG0180000287.01 | MsG0580029177.01 | -0.803267 | 3.781334e-49 | 1.891878e-46 |
| MsG0180000287.01 | MsG0680030960.01 | 0.823471 | 1.360674e-53 | 1.172652e-50 |
| MsG0180000287.01 | MsG0680032343.01 | 0.831439 | 1.668104e-55 | 1.810240e-52 |
| MsG0180000287.01 | MsG0680033015.01 | 0.809909 | 1.498568e-50 | 8.909021e-48 |
| MsG0180000287.01 | MsG0680033347.01 | 0.846381 | 2.250843e-59 | 3.867179e-56 |
| MsG0180000287.01 | MsG0680035584.01 | -0.810754 | 9.847940e-51 | 5.986789e-48 |
| MsG0180000287.01 | MsG0780036208.01 | 0.816732 | 4.752257e-52 | 3.394514e-49 |
| MsG0180000287.01 | MsG0780036427.01 | 0.800642 | 1.309401e-48 | 6.128647e-46 |
| MsG0180000287.01 | MsG0780036472.01 | 0.800620 | 1.323468e-48 | 6.190874e-46 |
| MsG0180000287.01 | MsG0780038519.01 | 0.807018 | 6.204517e-50 | 3.418242e-47 |
| MsG0180000287.01 | MsG0780039237.01 | 0.819923 | 8.999107e-53 | 7.019841e-50 |
| MsG0180000287.01 | MsG0780039431.01 | 0.816519 | 5.304082e-52 | 3.766644e-49 |
| MsG0180000287.01 | MsG0780039836.01 | -0.807929 | 3.975149e-50 | 2.242522e-47 |
| MsG0180000287.01 | MsG0780039839.01 | -0.802900 | 4.504468e-49 | 2.232665e-46 |
| MsG0180000287.01 | MsG0780039916.01 | 0.810160 | 1.323645e-50 | 7.920437e-48 |
| MsG0180000287.01 | MsG0780040034.01 | 0.805420 | 1.346429e-49 | 7.119976e-47 |
| MsG0180000287.01 | MsG0780040977.01 | 0.818211 | 2.206569e-52 | 1.641338e-49 |
| MsG0180000287.01 | MsG0780041036.01 | 0.802434 | 5.619621e-49 | 2.752589e-46 |
| MsG0180000287.01 | MsG0780041180.01 | 0.809818 | 1.567824e-50 | 9.298533e-48 |
| MsG0180000287.01 | MsG0780041316.01 | 0.804671 | 1.931715e-49 | 1.001991e-46 |
| MsG0180000287.01 | MsG0780041397.01 | 0.803036 | 4.221479e-49 | 2.099590e-46 |
| MsG0180000287.01 | MsG0780041783.01 | -0.800769 | 1.233745e-48 | 5.792913e-46 |
| MsG0180000287.01 | MsG0880042122.01 | 0.822119 | 2.808635e-53 | 2.329427e-50 |
| MsG0180000287.01 | MsG0880042740.01 | 0.800004 | 1.766146e-48 | 8.133471e-46 |
| MsG0180000287.01 | MsG0880043217.01 | 0.812694 | 3.726792e-51 | 2.385639e-48 |
| MsG0180000287.01 | MsG0880043481.01 | 0.800171 | 1.633159e-48 | 7.552930e-46 |
| MsG0180000287.01 | MsG0880043550.01 | 0.825996 | 3.456422e-54 | 3.201069e-51 |
| MsG0180000287.01 | MsG0880043607.01 | 0.809415 | 1.913755e-50 | 1.122977e-47 |
| MsG0180000287.01 | MsG0880045369.01 | -0.800519 | 1.387411e-48 | 6.473441e-46 |
| MsG0180000287.01 | MsG0880045385.01 | -0.809730 | 1.637955e-50 | 9.692162e-48 |
| MsG0180000287.01 | MsG0880045645.01 | 0.800300 | 1.537229e-48 | 7.133126e-46 |
| MsG0180000287.01 | MsG0880045851.01 | 0.811343 | 7.341220e-51 | 4.532616e-48 |
| MsG0180000287.01 | MsG0880045852.01 | 0.802501 | 5.445094e-49 | 2.671504e-46 |
| MsG0180000287.01 | MsG0880046271.01 | 0.837062 | 6.485740e-57 | 8.328188e-54 |
| MsG0180000287.01 | MsG0880046273.01 | 0.813370 | 2.649316e-51 | 1.726972e-48 |
| MsG0180000287.01 | MsG0880046463.01 | 0.803519 | 3.353406e-49 | 1.688748e-46 |
| MsG0180000287.01 | MsG0880047714.01 | 0.816661 | 4.930320e-52 | 3.514945e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000287.01.T01 | MTR_1g019040 | 92.634 | 448 | 28 | 4 | 1 | 445 | 1 | 446 | 0.0 | 813 |
| MsG0180000287.01.T01 | MTR_3g102120 | 58.936 | 470 | 157 | 11 | 7 | 443 | 3 | 469 | 6.35e-179 | 508 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000287.01.T01 | AT4G06536 | 52.071 | 169 | 63 | 3 | 279 | 437 | 3 | 163 | 7.88e-52 | 172 |
| MsG0180000287.01.T01 | AT4G06534 | 37.751 | 249 | 124 | 9 | 48 | 282 | 72 | 303 | 1.55e-33 | 130 |
Find 92 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGATTGTTTCTTTCT+TGG | 0.143166 | 1:-3884120 | None:intergenic |
| AAATCACTATATTGCATTCT+TGG | 0.179683 | 1:+3884536 | MsG0180000287.01.T01:CDS |
| GGCTGCAAATATAGACATTA+TGG | 0.216280 | 1:+3885350 | MsG0180000287.01.T01:CDS |
| CAAGTGTTGCTTCTGTTATT+AGG | 0.239162 | 1:+3884449 | MsG0180000287.01.T01:CDS |
| AAATATAGACATTATGGTTT+TGG | 0.262776 | 1:+3885356 | MsG0180000287.01.T01:CDS |
| GCTTCTGATGCTATAGAAAA+TGG | 0.269588 | 1:+3884205 | MsG0180000287.01.T01:CDS |
| TACCTCCACATGATCCTAAT+AGG | 0.278603 | 1:-3884294 | None:intergenic |
| TCAACTTCATCAACCCTATT+AGG | 0.286336 | 1:+3884280 | MsG0180000287.01.T01:CDS |
| GAGTTGAAACCTATGCTTCT+AGG | 0.290274 | 1:-3884757 | None:intergenic |
| GGTGTTCTTTGTGCATTTGC+AGG | 0.344174 | 1:-3885411 | None:intergenic |
| AATGGTTCTGTGTTTCTTGA+TGG | 0.346638 | 1:+3884781 | MsG0180000287.01.T01:CDS |
| AGGACCTCTCTGCCTCTTCC+TGG | 0.361354 | 1:+3884469 | MsG0180000287.01.T01:CDS |
| CTTCACATTAAACTCAGAAT+TGG | 0.364848 | 1:+3885272 | MsG0180000287.01.T01:CDS |
| ATGTTGTCATTGGGGTTAAC+AGG | 0.367061 | 1:+3884697 | MsG0180000287.01.T01:CDS |
| GGTGTTCCTCTAAGAGTTCC+AGG | 0.390256 | 1:+3884727 | MsG0180000287.01.T01:CDS |
| CATTCCCCTGGTGCTGCACT+TGG | 0.393098 | 1:+3885462 | MsG0180000287.01.T01:CDS |
| TGTGGAGGTAGTGATGAATT+TGG | 0.393678 | 1:+3884307 | MsG0180000287.01.T01:CDS |
| TAAGTTCCTGGAACTCTTAG+AGG | 0.405025 | 1:-3884733 | None:intergenic |
| TTGGTTGTGGATTTGATCCT+AGG | 0.407304 | 1:+3885235 | MsG0180000287.01.T01:CDS |
| CTTGGAATGGAGATTATGAT+TGG | 0.430140 | 1:+3884554 | MsG0180000287.01.T01:CDS |
| GTTGAAGAAATGAAAGTTGA+AGG | 0.430549 | 1:+3884649 | MsG0180000287.01.T01:CDS |
| ATACTCAGTGTCCTTATCAT+AGG | 0.434137 | 1:+3885659 | MsG0180000287.01.T01:CDS |
| ACCACATGAAAACAAACAAA+AGG | 0.437599 | 1:-3884030 | None:intergenic |
| ACAAAAGGAAATAACATCAA+TGG | 0.438406 | 1:+3885552 | MsG0180000287.01.T01:CDS |
| ACCTCCACATGATCCTAATA+GGG | 0.444884 | 1:-3884293 | None:intergenic |
| TCTATATTTGCAGCCAAAGT+TGG | 0.444908 | 1:-3885342 | None:intergenic |
| ACACTTGTAGAACTGTTGTT+TGG | 0.449080 | 1:-3884433 | None:intergenic |
| ATAGCATCAGAAGCTAGATA+TGG | 0.452505 | 1:-3884196 | None:intergenic |
| TATGATAAGGACACTGAGTA+TGG | 0.460716 | 1:-3885657 | None:intergenic |
| CAAAAGGAAATAACATCAAT+GGG | 0.463014 | 1:+3885553 | MsG0180000287.01.T01:CDS |
| AAAATGAGGCTGAAATAAGC+TGG | 0.463554 | 1:+3884335 | MsG0180000287.01.T01:CDS |
| ATGCTTCTAGGATAAGTTCC+TGG | 0.477399 | 1:-3884745 | None:intergenic |
| GAAGAAAAGATTTGTTCAGC+TGG | 0.481259 | 1:+3884164 | MsG0180000287.01.T01:CDS |
| GAAACTGTGATGTTGTCATT+GGG | 0.481926 | 1:+3884688 | MsG0180000287.01.T01:CDS |
| ACTGATACAGTAATTGGTTG+TGG | 0.482195 | 1:+3885222 | MsG0180000287.01.T01:CDS |
| ATGATTGGTGGAAGAAGAGA+AGG | 0.484206 | 1:+3884569 | MsG0180000287.01.T01:CDS |
| ACTATATTGCATTCTTGGAA+TGG | 0.486469 | 1:+3884541 | MsG0180000287.01.T01:CDS |
| TGAAACTGTGATGTTGTCAT+TGG | 0.490342 | 1:+3884687 | MsG0180000287.01.T01:CDS |
| AATCTGAATTGATGAGTCAA+TGG | 0.490494 | 1:+3883957 | None:intergenic |
| GGTGCTATGAAGCATGGATT+TGG | 0.494107 | 1:-3885432 | None:intergenic |
| GCTATTATAGCAGCCTTAGT+TGG | 0.494202 | 1:+3883995 | MsG0180000287.01.T01:CDS |
| CAGAATCGATCGGCAACAAA+AGG | 0.495388 | 1:+3885537 | MsG0180000287.01.T01:CDS |
| GTGGGAACTGATACAGTAAT+TGG | 0.504282 | 1:+3885216 | MsG0180000287.01.T01:CDS |
| ATCAAGAAACACAGAACCAT+TGG | 0.508071 | 1:-3884779 | None:intergenic |
| ATTTCAAAATAAGCTTCTTG+TGG | 0.514560 | 1:-3884517 | None:intergenic |
| TGATTGGTGGAAGAAGAGAA+GGG | 0.514573 | 1:+3884570 | MsG0180000287.01.T01:CDS |
| TCAAATCCAAGTGCAGCACC+AGG | 0.515251 | 1:-3885468 | None:intergenic |
| CACTCCACTATGTCCAACTT+TGG | 0.516191 | 1:+3885329 | MsG0180000287.01.T01:CDS |
| TTGGGGTTAACAGGTGGAGG+AGG | 0.519908 | 1:+3884706 | MsG0180000287.01.T01:CDS |
| GCAGCCAAAGTTGGACATAG+TGG | 0.521249 | 1:-3885333 | None:intergenic |
| GACAAAACTACTATTGAAGA+TGG | 0.525613 | 1:+3884598 | MsG0180000287.01.T01:CDS |
| ACTTGGATTTGATGACAGTA+AGG | 0.526846 | 1:+3885479 | MsG0180000287.01.T01:CDS |
| GGAACTTATCCTAGAAGCAT+AGG | 0.531788 | 1:+3884748 | MsG0180000287.01.T01:CDS |
| GGTTGAAATACATCCAACTA+AGG | 0.537760 | 1:-3884008 | None:intergenic |
| CAGTAAGGAACTATTCTCCA+TGG | 0.538917 | 1:+3885494 | MsG0180000287.01.T01:CDS |
| TGACTTATTTGAAATTGTGT+TGG | 0.550933 | 1:+3885617 | MsG0180000287.01.T01:CDS |
| AAGCTTCTTGTGGAAATGCA+TGG | 0.553279 | 1:-3884507 | None:intergenic |
| AGCATAGGTTTCAACTCCAA+TGG | 0.557174 | 1:+3884763 | MsG0180000287.01.T01:CDS |
| TCATTGGGGTTAACAGGTGG+AGG | 0.559181 | 1:+3884703 | MsG0180000287.01.T01:CDS |
| GAGTGCTGAATTCCTCTGAT+TGG | 0.561029 | 1:-3885311 | None:intergenic |
| CCACATGAAAACAAACAAAA+GGG | 0.561806 | 1:-3884029 | None:intergenic |
| GGAATGGAGATTATGATTGG+TGG | 0.571400 | 1:+3884557 | MsG0180000287.01.T01:CDS |
| GCACCACTTGTACATTCCCC+TGG | 0.572742 | 1:+3885450 | MsG0180000287.01.T01:CDS |
| CGGAGAATCAATCCTTCCCA+TGG | 0.577675 | 1:-3885511 | None:intergenic |
| TTATTTGAAATTGTGTTGGA+TGG | 0.586901 | 1:+3885621 | MsG0180000287.01.T01:CDS |
| GAAGATGGTTCAAATGATGA+AGG | 0.592118 | 1:+3884613 | MsG0180000287.01.T01:CDS |
| TTGTCATTGGGGTTAACAGG+TGG | 0.592233 | 1:+3884700 | MsG0180000287.01.T01:CDS |
| GATCAAAGAAGAAAGAGCAA+AGG | 0.592962 | 1:+3884142 | MsG0180000287.01.T01:CDS |
| AAATGAGGCTGAAATAAGCT+GGG | 0.599661 | 1:+3884336 | MsG0180000287.01.T01:CDS |
| GATTGGTGGAAGAAGAGAAG+GGG | 0.600350 | 1:+3884571 | MsG0180000287.01.T01:CDS |
| GTCTGAGAAAGCACAGTGGG+TGG | 0.603653 | 1:+3885197 | MsG0180000287.01.T01:CDS |
| CTGATGCTATAGAAAATGGA+TGG | 0.609254 | 1:+3884209 | MsG0180000287.01.T01:CDS |
| CAGAAGATAAGACTCAATCC+TGG | 0.610487 | 1:+3884388 | MsG0180000287.01.T01:CDS |
| TGAGTCTGAGAAAGCACAGT+GGG | 0.611118 | 1:+3885194 | MsG0180000287.01.T01:CDS |
| AGGAACTATTCTCCATGGGA+AGG | 0.613811 | 1:+3885499 | MsG0180000287.01.T01:CDS |
| TGAATTTGGAAGAGAAAATG+AGG | 0.614435 | 1:+3884321 | MsG0180000287.01.T01:CDS |
| AGTAAGGAACTATTCTCCAT+GGG | 0.620026 | 1:+3885495 | MsG0180000287.01.T01:CDS |
| TCAACCCTATTAGGATCATG+TGG | 0.623506 | 1:+3884289 | MsG0180000287.01.T01:CDS |
| TTCTCCGTGGCAGAATCGAT+CGG | 0.625199 | 1:+3885527 | MsG0180000287.01.T01:CDS |
| ACAAGTGGTGCTATGAAGCA+TGG | 0.628856 | 1:-3885438 | None:intergenic |
| ATAAGCTGGGAAGTGTGTCA+AGG | 0.639438 | 1:+3884349 | MsG0180000287.01.T01:CDS |
| TCTGAGAAAGCACAGTGGGT+GGG | 0.640383 | 1:+3885198 | MsG0180000287.01.T01:CDS |
| TGTAATCTACTGCCAATCAG+AGG | 0.648989 | 1:+3885299 | MsG0180000287.01.T01:CDS |
| GCACCAGGGGAATGTACAAG+TGG | 0.649933 | 1:-3885453 | None:intergenic |
| AGGACCAGGAAGAGGCAGAG+AGG | 0.652014 | 1:-3884473 | None:intergenic |
| CAAATCCAAGTGCAGCACCA+GGG | 0.652135 | 1:-3885467 | None:intergenic |
| AAATCCAAGTGCAGCACCAG+GGG | 0.660453 | 1:-3885466 | None:intergenic |
| GTTGCCGATCGATTCTGCCA+CGG | 0.661063 | 1:-3885531 | None:intergenic |
| AAACTGTGATGTTGTCATTG+GGG | 0.696690 | 1:+3884689 | MsG0180000287.01.T01:CDS |
| TGGGAAGGATTGATTCTCCG+TGG | 0.703647 | 1:+3885514 | MsG0180000287.01.T01:CDS |
| ACCCTATTAGGATCATGTGG+AGG | 0.719773 | 1:+3884292 | MsG0180000287.01.T01:CDS |
| TTGAGTCTGAGAAAGCACAG+TGG | 0.792356 | 1:+3885193 | MsG0180000287.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAATGTACATGACAA+GGG | - | Chr1:3884824-3884843 | None:intergenic | 20.0% |
| !! | TAAATAAAATGTACATGACA+AGG | - | Chr1:3884825-3884844 | None:intergenic | 20.0% |
| !! | TGACATATAATTGTAACATT+TGG | + | Chr1:3885149-3885168 | MsG0180000287.01.T01:intron | 20.0% |
| !! | TTCATAGATTACATTTCTAA+AGG | + | Chr1:3885016-3885035 | MsG0180000287.01.T01:intron | 20.0% |
| !!! | AAATATAGACATTATGGTTT+TGG | + | Chr1:3885356-3885375 | MsG0180000287.01.T01:CDS | 20.0% |
| !!! | ATTATGGTTTTGGTTAATTT+TGG | + | Chr1:3885366-3885385 | MsG0180000287.01.T01:CDS | 20.0% |
| !!! | TAGAATGTAATGTTTTCAAA+TGG | - | Chr1:3884848-3884867 | None:intergenic | 20.0% |
| ! | AAATCACTATATTGCATTCT+TGG | + | Chr1:3884536-3884555 | MsG0180000287.01.T01:CDS | 25.0% |
| ! | AATAAAATGTACATGACAAG+GGG | - | Chr1:3884823-3884842 | None:intergenic | 25.0% |
| ! | ACAAAAGGAAATAACATCAA+TGG | + | Chr1:3885552-3885571 | MsG0180000287.01.T01:CDS | 25.0% |
| ! | ATTTCAAAATAAGCTTCTTG+TGG | - | Chr1:3884520-3884539 | None:intergenic | 25.0% |
| ! | CAAAAGGAAATAACATCAAT+GGG | + | Chr1:3885553-3885572 | MsG0180000287.01.T01:CDS | 25.0% |
| !! | AAAAAACACTCTTTTAAACC+AGG | - | Chr1:3884409-3884428 | None:intergenic | 25.0% |
| !! | CAAAATGATTGTTTCTTTCT+TGG | - | Chr1:3884123-3884142 | None:intergenic | 25.0% |
| !! | TGACTTATTTGAAATTGTGT+TGG | + | Chr1:3885617-3885636 | MsG0180000287.01.T01:CDS | 25.0% |
| !! | TTATTTGAAATTGTGTTGGA+TGG | + | Chr1:3885621-3885640 | MsG0180000287.01.T01:CDS | 25.0% |
| !! | TTCTAAAGGTCAAAATATGT+TGG | + | Chr1:3885030-3885049 | MsG0180000287.01.T01:intron | 25.0% |
| ACCACATGAAAACAAACAAA+AGG | - | Chr1:3884033-3884052 | None:intergenic | 30.0% | |
| ACTATATTGCATTCTTGGAA+TGG | + | Chr1:3884541-3884560 | MsG0180000287.01.T01:CDS | 30.0% | |
| ATAAAATGTACATGACAAGG+GGG | - | Chr1:3884822-3884841 | None:intergenic | 30.0% | |
| ATGAAAGTTGAAGGAAAAAG+TGG | + | Chr1:3884658-3884677 | MsG0180000287.01.T01:CDS | 30.0% | |
| CCACATGAAAACAAACAAAA+GGG | - | Chr1:3884032-3884051 | None:intergenic | 30.0% | |
| CTTCACATTAAACTCAGAAT+TGG | + | Chr1:3885272-3885291 | MsG0180000287.01.T01:CDS | 30.0% | |
| GAAATGTAATCTATGAAGCA+CGG | - | Chr1:3885013-3885032 | None:intergenic | 30.0% | |
| GACAAAACTACTATTGAAGA+TGG | + | Chr1:3884598-3884617 | MsG0180000287.01.T01:CDS | 30.0% | |
| GTCAAAATATGTTGGTCAAT+AGG | + | Chr1:3885038-3885057 | MsG0180000287.01.T01:intron | 30.0% | |
| GTTGAAGAAATGAAAGTTGA+AGG | + | Chr1:3884649-3884668 | MsG0180000287.01.T01:CDS | 30.0% | |
| TAATTGTAACATTTGGTCAC+AGG | + | Chr1:3885156-3885175 | MsG0180000287.01.T01:intron | 30.0% | |
| TCTCAAATTATTATCCGTGT+TGG | + | Chr1:3884976-3884995 | MsG0180000287.01.T01:intron | 30.0% | |
| TGAAAGTTGAAGGAAAAAGT+GGG | + | Chr1:3884659-3884678 | MsG0180000287.01.T01:CDS | 30.0% | |
| TGAATTTGGAAGAGAAAATG+AGG | + | Chr1:3884321-3884340 | MsG0180000287.01.T01:CDS | 30.0% | |
| ! | AAGAAAACTTTCTTTTGCCT+AGG | - | Chr1:3885255-3885274 | None:intergenic | 30.0% |
| !! | TTTTGTTTGTTTTCATGTGG+TGG | + | Chr1:3884032-3884051 | MsG0180000287.01.T01:CDS | 30.0% |
| AAAATGAGGCTGAAATAAGC+TGG | + | Chr1:3884335-3884354 | MsG0180000287.01.T01:CDS | 35.0% | |
| AAACTGTGATGTTGTCATTG+GGG | + | Chr1:3884689-3884708 | MsG0180000287.01.T01:CDS | 35.0% | |
| AAATGAGGCTGAAATAAGCT+GGG | + | Chr1:3884336-3884355 | MsG0180000287.01.T01:CDS | 35.0% | |
| ACACTTGTAGAACTGTTGTT+TGG | - | Chr1:3884436-3884455 | None:intergenic | 35.0% | |
| ACTGATACAGTAATTGGTTG+TGG | + | Chr1:3885222-3885241 | MsG0180000287.01.T01:CDS | 35.0% | |
| AGTAAGGAACTATTCTCCAT+GGG | + | Chr1:3885495-3885514 | MsG0180000287.01.T01:CDS | 35.0% | |
| ATAGCATCAGAAGCTAGATA+TGG | - | Chr1:3884199-3884218 | None:intergenic | 35.0% | |
| ATCAAGAAACACAGAACCAT+TGG | - | Chr1:3884782-3884801 | None:intergenic | 35.0% | |
| CTGATGCTATAGAAAATGGA+TGG | + | Chr1:3884209-3884228 | MsG0180000287.01.T01:CDS | 35.0% | |
| CTTGGAATGGAGATTATGAT+TGG | + | Chr1:3884554-3884573 | MsG0180000287.01.T01:CDS | 35.0% | |
| GAAACTGTGATGTTGTCATT+GGG | + | Chr1:3884688-3884707 | MsG0180000287.01.T01:CDS | 35.0% | |
| GAAGATGGTTCAAATGATGA+AGG | + | Chr1:3884613-3884632 | MsG0180000287.01.T01:CDS | 35.0% | |
| GATCAAAGAAGAAAGAGCAA+AGG | + | Chr1:3884142-3884161 | MsG0180000287.01.T01:CDS | 35.0% | |
| GCTTCTGATGCTATAGAAAA+TGG | + | Chr1:3884205-3884224 | MsG0180000287.01.T01:CDS | 35.0% | |
| GGCTGCAAATATAGACATTA+TGG | + | Chr1:3885350-3885369 | MsG0180000287.01.T01:CDS | 35.0% | |
| GGTTGAAATACATCCAACTA+AGG | - | Chr1:3884011-3884030 | None:intergenic | 35.0% | |
| TATGATAAGGACACTGAGTA+TGG | - | Chr1:3885660-3885679 | None:intergenic | 35.0% | |
| TCAACTTCATCAACCCTATT+AGG | + | Chr1:3884280-3884299 | MsG0180000287.01.T01:CDS | 35.0% | |
| TCTATATTTGCAGCCAAAGT+TGG | - | Chr1:3885345-3885364 | None:intergenic | 35.0% | |
| TGAAACTGTGATGTTGTCAT+TGG | + | Chr1:3884687-3884706 | MsG0180000287.01.T01:CDS | 35.0% | |
| TGTAATCATAGATGTCGTGT+CGG | - | Chr1:3884940-3884959 | None:intergenic | 35.0% | |
| ! | AATGGTTCTGTGTTTCTTGA+TGG | + | Chr1:3884781-3884800 | MsG0180000287.01.T01:CDS | 35.0% |
| ! | ACTTGGATTTGATGACAGTA+AGG | + | Chr1:3885479-3885498 | MsG0180000287.01.T01:CDS | 35.0% |
| ! | GAAGAAAAGATTTGTTCAGC+TGG | + | Chr1:3884164-3884183 | MsG0180000287.01.T01:CDS | 35.0% |
| !! | CAAGTGTTGCTTCTGTTATT+AGG | + | Chr1:3884449-3884468 | MsG0180000287.01.T01:CDS | 35.0% |
| !! | CCCTTTTGTTTGTTTTCATG+TGG | + | Chr1:3884029-3884048 | MsG0180000287.01.T01:CDS | 35.0% |
| AAGCTTCTTGTGGAAATGCA+TGG | - | Chr1:3884510-3884529 | None:intergenic | 40.0% | |
| AATGCATGGTTCCCCAAAAA+AGG | - | Chr1:3884496-3884515 | None:intergenic | 40.0% | |
| ACCTCCACATGATCCTAATA+GGG | - | Chr1:3884296-3884315 | None:intergenic | 40.0% | |
| AGCATAGGTTTCAACTCCAA+TGG | + | Chr1:3884763-3884782 | MsG0180000287.01.T01:CDS | 40.0% | |
| ATGATTGGTGGAAGAAGAGA+AGG | + | Chr1:3884569-3884588 | MsG0180000287.01.T01:CDS | 40.0% | |
| ATGTTGTCATTGGGGTTAAC+AGG | + | Chr1:3884697-3884716 | MsG0180000287.01.T01:CDS | 40.0% | |
| CAGAAGATAAGACTCAATCC+TGG | + | Chr1:3884388-3884407 | MsG0180000287.01.T01:CDS | 40.0% | |
| CAGTAAGGAACTATTCTCCA+TGG | + | Chr1:3885494-3885513 | MsG0180000287.01.T01:CDS | 40.0% | |
| GAGTTGAAACCTATGCTTCT+AGG | - | Chr1:3884760-3884779 | None:intergenic | 40.0% | |
| GCTATTATAGCAGCCTTAGT+TGG | + | Chr1:3883995-3884014 | MsG0180000287.01.T01:CDS | 40.0% | |
| GGAATGGAGATTATGATTGG+TGG | + | Chr1:3884557-3884576 | MsG0180000287.01.T01:CDS | 40.0% | |
| GTGGGAACTGATACAGTAAT+TGG | + | Chr1:3885216-3885235 | MsG0180000287.01.T01:CDS | 40.0% | |
| TAAGTTCCTGGAACTCTTAG+AGG | - | Chr1:3884736-3884755 | None:intergenic | 40.0% | |
| TACCTCCACATGATCCTAAT+AGG | - | Chr1:3884297-3884316 | None:intergenic | 40.0% | |
| TCAACCCTATTAGGATCATG+TGG | + | Chr1:3884289-3884308 | MsG0180000287.01.T01:CDS | 40.0% | |
| TGATTGGTGGAAGAAGAGAA+GGG | + | Chr1:3884570-3884589 | MsG0180000287.01.T01:CDS | 40.0% | |
| TGTAATCTACTGCCAATCAG+AGG | + | Chr1:3885299-3885318 | MsG0180000287.01.T01:CDS | 40.0% | |
| TGTGGAGGTAGTGATGAATT+TGG | + | Chr1:3884307-3884326 | MsG0180000287.01.T01:CDS | 40.0% | |
| TTGGTTGTGGATTTGATCCT+AGG | + | Chr1:3885235-3885254 | MsG0180000287.01.T01:CDS | 40.0% | |
| ! | GGAACTTATCCTAGAAGCAT+AGG | + | Chr1:3884748-3884767 | MsG0180000287.01.T01:CDS | 40.0% |
| !! | ATGCTTCTAGGATAAGTTCC+TGG | - | Chr1:3884748-3884767 | None:intergenic | 40.0% |
| ACCCTATTAGGATCATGTGG+AGG | + | Chr1:3884292-3884311 | MsG0180000287.01.T01:CDS | 45.0% | |
| AGGAACTATTCTCCATGGGA+AGG | + | Chr1:3885499-3885518 | MsG0180000287.01.T01:CDS | 45.0% | |
| CACTCCACTATGTCCAACTT+TGG | + | Chr1:3885329-3885348 | MsG0180000287.01.T01:CDS | 45.0% | |
| CAGAATCGATCGGCAACAAA+AGG | + | Chr1:3885537-3885556 | MsG0180000287.01.T01:CDS | 45.0% | |
| GATTGGTGGAAGAAGAGAAG+GGG | + | Chr1:3884571-3884590 | MsG0180000287.01.T01:CDS | 45.0% | |
| GGTGTTCTTTGTGCATTTGC+AGG | - | Chr1:3885414-3885433 | None:intergenic | 45.0% | |
| TTGTCATTGGGGTTAACAGG+TGG | + | Chr1:3884700-3884719 | MsG0180000287.01.T01:CDS | 45.0% | |
| ! | ATAAGCTGGGAAGTGTGTCA+AGG | + | Chr1:3884349-3884368 | MsG0180000287.01.T01:CDS | 45.0% |
| ! | CATAGATGTCGTGTCGGTAT+CGG | - | Chr1:3884934-3884953 | None:intergenic | 45.0% |
| ! | GGTGCTATGAAGCATGGATT+TGG | - | Chr1:3885435-3885454 | None:intergenic | 45.0% |
| ! | TGAGTCTGAGAAAGCACAGT+GGG | + | Chr1:3885194-3885213 | MsG0180000287.01.T01:CDS | 45.0% |
| ! | TTGAGTCTGAGAAAGCACAG+TGG | + | Chr1:3885193-3885212 | MsG0180000287.01.T01:CDS | 45.0% |
| !! | ACAAGTGGTGCTATGAAGCA+TGG | - | Chr1:3885441-3885460 | None:intergenic | 45.0% |
| !! | GAGTGCTGAATTCCTCTGAT+TGG | - | Chr1:3885314-3885333 | None:intergenic | 45.0% |
| !!! | CCTCTTCCTGGTCCTTTTTT+GGG | + | Chr1:3884481-3884500 | MsG0180000287.01.T01:CDS | 45.0% |
| !!! | CTCTTCCTGGTCCTTTTTTG+GGG | + | Chr1:3884482-3884501 | MsG0180000287.01.T01:CDS | 45.0% |
| AAATCCAAGTGCAGCACCAG+GGG | - | Chr1:3885469-3885488 | None:intergenic | 50.0% | |
| CAAATCCAAGTGCAGCACCA+GGG | - | Chr1:3885470-3885489 | None:intergenic | 50.0% | |
| CCCAAAAAAGGACCAGGAAG+AGG | - | Chr1:3884484-3884503 | None:intergenic | 50.0% | |
| CGGAGAATCAATCCTTCCCA+TGG | - | Chr1:3885514-3885533 | None:intergenic | 50.0% | |
| GCAGCCAAAGTTGGACATAG+TGG | - | Chr1:3885336-3885355 | None:intergenic | 50.0% | |
| TCAAATCCAAGTGCAGCACC+AGG | - | Chr1:3885471-3885490 | None:intergenic | 50.0% | |
| TCATTGGGGTTAACAGGTGG+AGG | + | Chr1:3884703-3884722 | MsG0180000287.01.T01:CDS | 50.0% | |
| TGGTTCCCCAAAAAAGGACC+AGG | - | Chr1:3884490-3884509 | None:intergenic | 50.0% | |
| TTCTCCGTGGCAGAATCGAT+CGG | + | Chr1:3885527-3885546 | MsG0180000287.01.T01:CDS | 50.0% | |
| ! | ACGCTTAATCCGAGGAGTGT+CGG | - | Chr1:3884878-3884897 | None:intergenic | 50.0% |
| ! | GGTGTTCCTCTAAGAGTTCC+AGG | + | Chr1:3884727-3884746 | MsG0180000287.01.T01:CDS | 50.0% |
| ! | TCTGAGAAAGCACAGTGGGT+GGG | + | Chr1:3885198-3885217 | MsG0180000287.01.T01:CDS | 50.0% |
| ! | TGGGAAGGATTGATTCTCCG+TGG | + | Chr1:3885514-3885533 | MsG0180000287.01.T01:CDS | 50.0% |
| !! | TATGAAGCACCGACACTCCT+CGG | + | Chr1:3884866-3884885 | MsG0180000287.01.T01:intron | 50.0% |
| !!! | GCCTCTTCCTGGTCCTTTTT+TGG | + | Chr1:3884480-3884499 | MsG0180000287.01.T01:CDS | 50.0% |
| CGGACACTGACACACCAACA+CGG | - | Chr1:3884993-3885012 | None:intergenic | 55.0% | |
| GATTAAGCGTGTCCCGGTGT+TGG | + | Chr1:3884888-3884907 | MsG0180000287.01.T01:intron | 55.0% | |
| GCACCACTTGTACATTCCCC+TGG | + | Chr1:3885450-3885469 | MsG0180000287.01.T01:CDS | 55.0% | |
| GCACCAGGGGAATGTACAAG+TGG | - | Chr1:3885456-3885475 | None:intergenic | 55.0% | |
| TCCTCGGATTAAGCGTGTCC+CGG | + | Chr1:3884882-3884901 | MsG0180000287.01.T01:intron | 55.0% | |
| TTGGGGTTAACAGGTGGAGG+AGG | + | Chr1:3884706-3884725 | MsG0180000287.01.T01:CDS | 55.0% | |
| ! | GTCTGAGAAAGCACAGTGGG+TGG | + | Chr1:3885197-3885216 | MsG0180000287.01.T01:CDS | 55.0% |
| ! | GTTGCCGATCGATTCTGCCA+CGG | - | Chr1:3885534-3885553 | None:intergenic | 55.0% |
| ACCGGGACACGCTTAATCCG+AGG | - | Chr1:3884886-3884905 | None:intergenic | 60.0% | |
| AGGACCAGGAAGAGGCAGAG+AGG | - | Chr1:3884476-3884495 | None:intergenic | 60.0% | |
| AGGACCTCTCTGCCTCTTCC+TGG | + | Chr1:3884469-3884488 | MsG0180000287.01.T01:CDS | 60.0% | |
| ! | TCCCGGTGTTGGACACGAGT+CGG | + | Chr1:3884899-3884918 | MsG0180000287.01.T01:intron | 60.0% |
| !! | ACCGACTCGTGTCCAACACC+GGG | - | Chr1:3884903-3884922 | None:intergenic | 60.0% |
| !! | CACCGACTCGTGTCCAACAC+CGG | - | Chr1:3884904-3884923 | None:intergenic | 60.0% |
| !! | CATTCCCCTGGTGCTGCACT+TGG | + | Chr1:3885462-3885481 | MsG0180000287.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 3883968 | 3885680 | 3883968 | ID=MsG0180000287.01;Name=MsG0180000287.01 |
| Chr1 | mRNA | 3883968 | 3885680 | 3883968 | ID=MsG0180000287.01.T01;Parent=MsG0180000287.01;Name=MsG0180000287.01.T01;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|445 |
| Chr1 | exon | 3883968 | 3884802 | 3883968 | ID=MsG0180000287.01.T01:exon:5852;Parent=MsG0180000287.01.T01 |
| Chr1 | exon | 3885178 | 3885680 | 3885178 | ID=MsG0180000287.01.T01:exon:5851;Parent=MsG0180000287.01.T01 |
| Chr1 | CDS | 3883968 | 3884802 | 3883968 | ID=MsG0180000287.01.T01:cds;Parent=MsG0180000287.01.T01 |
| Chr1 | CDS | 3885178 | 3885680 | 3885178 | ID=MsG0180000287.01.T01:cds;Parent=MsG0180000287.01.T01 |
| Gene Sequence |
| Protein sequence |
