Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002429.01.T01 | GAV64270.1 | 66.99 | 103 | 34 | 0 | 1 | 103 | 55 | 157 | 2.05E-34 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002429.01.T01 | P20560 | 51.948 | 77 | 37 | 0 | 1 | 77 | 1 | 77 | 6.81E-17 | 72 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002429.01.T01 | A0A1Q3B8H7 | 66.990 | 103 | 34 | 0 | 1 | 103 | 55 | 157 | 9.79e-35 | 126 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048781.01 | MsG0180002429.01 | 0.818262 | 2.148266e-52 | 1.600192e-49 |
| MsG0180002397.01 | MsG0180002429.01 | 0.801968 | 7.009586e-49 | 3.392607e-46 |
| MsG0180002421.01 | MsG0180002429.01 | 0.807942 | 3.950530e-50 | 2.229430e-47 |
| MsG0180002429.01 | MsG0180003485.01 | 0.825172 | 5.419778e-54 | 4.903030e-51 |
| MsG0180002429.01 | MsG0180003796.01 | 0.817749 | 2.806486e-52 | 2.061408e-49 |
| MsG0180002429.01 | MsG0180004126.01 | 0.818611 | 1.790954e-52 | 1.346941e-49 |
| MsG0180002429.01 | MsG0180006133.01 | 0.807966 | 3.903454e-50 | 2.204273e-47 |
| MsG0180002429.01 | MsG0280006540.01 | 0.821818 | 3.298115e-53 | 2.712247e-50 |
| MsG0180002429.01 | MsG0280006563.01 | 0.842381 | 2.676611e-58 | 4.051472e-55 |
| MsG0180002429.01 | MsG0280007359.01 | 0.806647 | 7.432136e-50 | 4.055247e-47 |
| MsG0180002429.01 | MsG0280011154.01 | 0.812411 | 4.296909e-51 | 2.730240e-48 |
| MsG0180002429.01 | MsG0380012788.01 | 0.816606 | 5.071517e-52 | 3.610167e-49 |
| MsG0180002429.01 | MsG0380015219.01 | 0.801239 | 9.891897e-49 | 4.700362e-46 |
| MsG0180002429.01 | MsG0380017164.01 | 0.812152 | 4.896015e-51 | 3.089082e-48 |
| MsG0180002429.01 | MsG0380017361.01 | 0.822803 | 1.948429e-53 | 1.647806e-50 |
| MsG0180002429.01 | MsG0480018526.01 | 0.824782 | 6.697676e-54 | 5.992068e-51 |
| MsG0180002429.01 | MsG0480018785.01 | 0.815398 | 9.435184e-52 | 6.497919e-49 |
| MsG0180002429.01 | MsG0480022991.01 | 0.813759 | 2.175283e-51 | 1.432946e-48 |
| MsG0180002429.01 | MsG0480023296.01 | 0.824670 | 7.119493e-54 | 6.349251e-51 |
| MsG0180002429.01 | MsG0480023672.01 | 0.810045 | 1.400892e-50 | 8.358264e-48 |
| MsG0180002429.01 | MsG0580024489.01 | 0.811233 | 7.755349e-51 | 4.774773e-48 |
| MsG0180002429.01 | MsG0580024657.01 | 0.806828 | 6.805984e-50 | 3.731247e-47 |
| MsG0180002429.01 | MsG0580024787.01 | 0.805232 | 1.474506e-49 | 7.759641e-47 |
| MsG0180002429.01 | MsG0580024813.01 | 0.804489 | 2.108108e-49 | 1.088330e-46 |
| MsG0180002429.01 | MsG0580025093.01 | 0.836052 | 1.172953e-56 | 1.461135e-53 |
| MsG0180002429.01 | MsG0580025177.01 | 0.816870 | 4.425198e-52 | 3.173022e-49 |
| MsG0180002429.01 | MsG0580028756.01 | 0.856369 | 3.371741e-62 | 8.029713e-59 |
| MsG0180002429.01 | MsG0580029591.01 | 0.813782 | 2.149682e-51 | 1.416982e-48 |
| MsG0180002429.01 | MsG0680031616.01 | 0.811665 | 6.249988e-51 | 3.891742e-48 |
| MsG0180002429.01 | MsG0680032773.01 | 0.803725 | 3.039291e-49 | 1.538616e-46 |
| MsG0180002429.01 | MsG0780037158.01 | 0.819975 | 8.758311e-53 | 6.841842e-50 |
| MsG0180002429.01 | MsG0780039861.01 | 0.824480 | 7.888723e-54 | 6.995843e-51 |
| MsG0180002429.01 | MsG0780040364.01 | 0.815235 | 1.025448e-51 | 7.030376e-49 |
| MsG0180002429.01 | MsG0780040620.01 | 0.816155 | 6.396460e-52 | 4.497664e-49 |
| MsG0180002429.01 | MsG0780041143.01 | 0.838755 | 2.382278e-57 | 3.221698e-54 |
| MsG0180002429.01 | MsG0780041340.01 | 0.807331 | 5.325609e-50 | 2.958100e-47 |
| MsG0180002429.01 | MsG0880044835.01 | 0.812776 | 3.576324e-51 | 2.294088e-48 |
| MsG0180002429.01 | MsG0880046885.01 | 0.826099 | 3.267122e-54 | 3.034473e-51 |
| MsG0180002429.01 | MsG0880047226.01 | 0.813229 | 2.844855e-51 | 1.847159e-48 |
| MsG0180002429.01 | MsG0880047466.01 | 0.810464 | 1.137733e-50 | 6.862812e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGATTGGGCGATGAAA+TGG | 0.358474 | 1:+38331124 | None:intergenic |
| AGCAAATGCAACGATTCTTT+CGG | 0.411380 | 1:-38331169 | MsG0180002429.01.T01:CDS |
| GTTGCATTTGCTTGTGTTGA+AGG | 0.436600 | 1:+38331180 | None:intergenic |
| GAAAATCGCACAGAAGCTTC+CGG | 0.456616 | 1:-38331214 | MsG0180002429.01.T01:CDS |
| TGAAATAAGCTCGTTTGAGA+AGG | 0.487505 | 1:-38331271 | MsG0180002429.01.T01:CDS |
| GAGCTTATTTCACGCGACGA+AGG | 0.572724 | 1:+38331282 | None:intergenic |
| TCGTTTGAGAAGGTTAATCC+CGG | 0.587906 | 1:-38331261 | MsG0180002429.01.T01:CDS |
| GCTAAGTTCAGAGCGCGTGA+AGG | 0.615081 | 1:+38331353 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATCGCAAAAAAAGCTGATT+GGG | + | Chr1:38331357-38331376 | None:intergenic | 30.0% | |
| !!! | ATTTTCTGGTTGAAAAAACG+TGG | + | Chr1:38331239-38331258 | None:intergenic | 30.0% |
| AAAAAACGTGGTTTAATGCC+GGG | + | Chr1:38331227-38331246 | None:intergenic | 35.0% | |
| AGCAAATGCAACGATTCTTT+CGG | - | Chr1:38331298-38331317 | MsG0180002429.01.T01:CDS | 35.0% | |
| GAAAAAACGTGGTTTAATGC+CGG | + | Chr1:38331228-38331247 | None:intergenic | 35.0% | |
| GTATCGCAAAAAAAGCTGAT+TGG | + | Chr1:38331358-38331377 | None:intergenic | 35.0% | |
| TGAAATAAGCTCGTTTGAGA+AGG | - | Chr1:38331196-38331215 | MsG0180002429.01.T01:CDS | 35.0% | |
| ! | GTTGAAGGAATCTTTTTCTC+CGG | + | Chr1:38331275-38331294 | None:intergenic | 35.0% |
| !!! | TCAGCTTTTTTTGCGATACA+TGG | - | Chr1:38331359-38331378 | MsG0180002429.01.T01:CDS | 35.0% |
| GTTGCATTTGCTTGTGTTGA+AGG | + | Chr1:38331290-38331309 | None:intergenic | 40.0% | |
| TCGTTTGAGAAGGTTAATCC+CGG | - | Chr1:38331206-38331225 | MsG0180002429.01.T01:CDS | 40.0% | |
| AAGCTGATTGGGCGATGAAA+TGG | + | Chr1:38331346-38331365 | None:intergenic | 45.0% | |
| GAAAATCGCACAGAAGCTTC+CGG | - | Chr1:38331253-38331272 | MsG0180002429.01.T01:CDS | 45.0% | |
| ! | GAAGCTTCTGTGCGATTTTC+TGG | + | Chr1:38331253-38331272 | None:intergenic | 45.0% |
| GAGCTTATTTCACGCGACGA+AGG | + | Chr1:38331188-38331207 | None:intergenic | 50.0% | |
| GCTAAGTTCAGAGCGCGTGA+AGG | + | Chr1:38331117-38331136 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 38331089 | 38331400 | 38331089 | ID=MsG0180002429.01;Name=MsG0180002429.01 |
| Chr1 | mRNA | 38331089 | 38331400 | 38331089 | ID=MsG0180002429.01.T01;Parent=MsG0180002429.01;Name=MsG0180002429.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|103 |
| Chr1 | exon | 38331089 | 38331400 | 38331089 | ID=MsG0180002429.01.T01:exon:13032;Parent=MsG0180002429.01.T01 |
| Chr1 | CDS | 38331089 | 38331400 | 38331089 | ID=MsG0180002429.01.T01:cds;Parent=MsG0180002429.01.T01 |
| Gene Sequence |
| Protein sequence |
