AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037158.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037158.01.T01 MTR_0268s0080 98.718 312 4 0 1 312 19 330 0.0 638
MsG0780037158.01.T01 MTR_1g050472 98.077 312 6 0 1 312 19 330 0.0 634
MsG0780037158.01.T01 MTR_7g029110 97.756 312 7 0 1 312 19 330 0.0 631
MsG0780037158.01.T01 MTR_8g469010 92.628 312 18 1 1 312 19 325 0.0 598
MsG0780037158.01.T01 MTR_4g117370 77.564 312 69 1 1 312 31 341 0.0 516
MsG0780037158.01.T01 MTR_6g005270 67.442 43 14 0 247 289 25 67 9.84e-14 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037158.01.T01 AT3G27060 84.076 314 48 2 1 312 19 332 0.0 525
MsG0780037158.01.T01 AT3G23580 77.564 312 69 1 1 312 31 341 0.0 506

Find 52 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTACATTTCCCAGATTT+TGG 0.151339 7:+20792367 None:intergenic
ATTTGTTGCTGATAGATTAT+TGG 0.266016 7:-20791658 MsG0780037158.01.T01:CDS
AATTCCATGTATCGCGAAAA+AGG 0.267050 7:-20792033 MsG0780037158.01.T01:CDS
CAATCCAATACCCCAAAATC+TGG 0.320986 7:-20792379 MsG0780037158.01.T01:CDS
GTTGCATTTGCTTGTGTAGA+AGG 0.323501 7:-20791957 MsG0780037158.01.T01:CDS
TGCAATCTGGAAACCGTAAA+AGG 0.337326 7:+20792151 None:intergenic
ATTTCTTTGAGAAGCGCGTT+GGG 0.337568 7:-20791554 MsG0780037158.01.T01:CDS
TTTGTTGCTGATAGATTATT+GGG 0.363819 7:-20791657 MsG0780037158.01.T01:CDS
CGAAATAAGCTCGTTTGAAA+AGG 0.374698 7:+20791866 None:intergenic
AATCGCTTATATACGTTTCA+AGG 0.387103 7:+20792093 None:intergenic
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 7:-20791608 MsG0780037158.01.T01:CDS
ACCGTAAAAGGCACGTGCTT+CGG 0.395979 7:+20792163 None:intergenic
GCGCTTCCTTGCGCGTTGGT+TGG 0.401864 7:-20791714 MsG0780037158.01.T01:CDS
AGGCTGATTGGGCAATGAAA+TGG 0.412010 7:-20792013 MsG0780037158.01.T01:CDS
TGCGCGTTGGTTGGAATGAA+TGG 0.417042 7:-20791705 MsG0780037158.01.T01:CDS
TCAGAATCCGTTTGATTGGA+TGG 0.443597 7:-20791604 MsG0780037158.01.T01:CDS
TCCGTCTGAGGCGGCGAAGA+AGG 0.447235 7:+20792235 None:intergenic
GCCTTCTTCGCCGCCTCAGA+CGG 0.453049 7:-20792236 MsG0780037158.01.T01:CDS
CTGTTATGAATAGCTTGAAT+GGG 0.453833 7:-20791515 MsG0780037158.01.T01:CDS
TCTGTTATGAATAGCTTGAA+TGG 0.458759 7:-20791516 MsG0780037158.01.T01:CDS
CGACGCGCTTCCTTGCGCGT+TGG 0.462960 7:-20791718 MsG0780037158.01.T01:CDS
GGCGGTCCAGAATGAGGCTT+CGG 0.476778 7:+20792340 None:intergenic
ATGGAATTGATTTCGCTTCA+AGG 0.479094 7:-20791585 MsG0780037158.01.T01:CDS
ATCAATTCCATCCAATCAAA+CGG 0.480364 7:+20791597 None:intergenic
GCAATGCAGTTGAAATTGAA+AGG 0.492366 7:-20791752 MsG0780037158.01.T01:CDS
GGAGGAAAGATCGACTTCTT+CGG 0.499480 7:+20792319 None:intergenic
GGGTGAACTTGGTTGTGAAA+AGG 0.510958 7:-20791637 MsG0780037158.01.T01:CDS
TCGTTTGAAAAGGTTAATCC+TGG 0.512613 7:+20791876 None:intergenic
GATAGATTATTGGGTGAACT+TGG 0.513434 7:-20791648 MsG0780037158.01.T01:CDS
GTTGTTCCAGTGCTTGAGAT+CGG 0.515525 7:+20792298 None:intergenic
AATTTCTTTGAGAAGCGCGT+TGG 0.523856 7:-20791555 MsG0780037158.01.T01:CDS
TTCTTCGGCGGTCCAGAATG+AGG 0.524296 7:+20792334 None:intergenic
TTCGAGGACAATTCCGTCTG+AGG 0.536756 7:+20792223 None:intergenic
TTTCCTCCGATCTCAAGCAC+TGG 0.537267 7:-20792304 MsG0780037158.01.T01:CDS
CAATGCAGTTGAAATTGAAA+GGG 0.547072 7:-20791751 MsG0780037158.01.T01:CDS
TGGAATTGATTTCGCTTCAA+GGG 0.551257 7:-20791584 MsG0780037158.01.T01:CDS
CGAGGACGTGTGAGATGAAG+TGG 0.551534 7:+20792258 None:intergenic
GAGCTTATTTCGCGTGACGA+AGG 0.555351 7:-20791855 MsG0780037158.01.T01:CDS
AATCCAATACCCCAAAATCT+GGG 0.556684 7:-20792378 MsG0780037158.01.T01:CDS
ATTGTCCTCGAAAATCTCGC+TGG 0.568595 7:-20792212 MsG0780037158.01.T01:CDS
CACTGGAACAACCTCACAGA+CGG 0.586656 7:-20792287 MsG0780037158.01.T01:CDS
TTCATTCCAACCAACGCGCA+AGG 0.590197 7:+20791708 None:intergenic
TTTCTTTGAGAAGCGCGTTG+GGG 0.598671 7:-20791553 MsG0780037158.01.T01:CDS
GAAGTGAATACATCTCAGAA+TGG 0.599540 7:+20792117 None:intergenic
GAGGACAATTCCGTCTGAGG+CGG 0.603534 7:+20792226 None:intergenic
ATGAATAGCTTGAATGGGAA+TGG 0.603815 7:-20791510 MsG0780037158.01.T01:CDS
CTGAGGCGGCGAAGAAGGCG+AGG 0.610767 7:+20792240 None:intergenic
ACTAACTTCAGAGCGTGTGA+AGG 0.618034 7:-20791784 MsG0780037158.01.T01:CDS
GTTCCAGTGCTTGAGATCGG+AGG 0.623496 7:+20792301 None:intergenic
GCGTTGGTTGGAATGAATGG+TGG 0.654887 7:-20791702 MsG0780037158.01.T01:CDS
GGAAAGATCGACTTCTTCGG+CGG 0.687608 7:+20792322 None:intergenic
AGTGGCGTTCGCCGTCTGTG+AGG 0.694180 7:+20792276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGTTGCTGATAGATTATT+GGG - Chr7:20792199-20792218 MsG0780037158.01.T01:CDS 25.0%
!! ATTTGTTGCTGATAGATTAT+TGG - Chr7:20792198-20792217 MsG0780037158.01.T01:CDS 25.0%
AATCGCTTATATACGTTTCA+AGG + Chr7:20791766-20791785 None:intergenic 30.0%
ATCAATTCCATCCAATCAAA+CGG + Chr7:20792262-20792281 None:intergenic 30.0%
CAATGCAGTTGAAATTGAAA+GGG - Chr7:20792105-20792124 MsG0780037158.01.T01:CDS 30.0%
CTGTTATGAATAGCTTGAAT+GGG - Chr7:20792341-20792360 MsG0780037158.01.T01:CDS 30.0%
TCTGTTATGAATAGCTTGAA+TGG - Chr7:20792340-20792359 MsG0780037158.01.T01:CDS 30.0%
! TTGTACATTTCCCAGATTTT+GGG + Chr7:20791491-20791510 None:intergenic 30.0%
! TTTGTACATTTCCCAGATTT+TGG + Chr7:20791492-20791511 None:intergenic 30.0%
!!! ATTTTCTGGTTGAAAAAACG+CGG - Chr7:20791950-20791969 MsG0780037158.01.T01:CDS 30.0%
!!! TGCTGATTTTTGCTTTAGTT+TGG - Chr7:20792372-20792391 MsG0780037158.01.T01:CDS 30.0%
AATCCAATACCCCAAAATCT+GGG - Chr7:20791478-20791497 MsG0780037158.01.T01:CDS 35.0%
AATTCCATGTATCGCGAAAA+AGG - Chr7:20791823-20791842 MsG0780037158.01.T01:CDS 35.0%
ATGAATAGCTTGAATGGGAA+TGG - Chr7:20792346-20792365 MsG0780037158.01.T01:CDS 35.0%
ATGTTCAGAATCCGTTTGAT+TGG - Chr7:20792248-20792267 MsG0780037158.01.T01:CDS 35.0%
CGAAATAAGCTCGTTTGAAA+AGG + Chr7:20791993-20792012 None:intergenic 35.0%
GAAGTGAATACATCTCAGAA+TGG + Chr7:20791742-20791761 None:intergenic 35.0%
GATAGATTATTGGGTGAACT+TGG - Chr7:20792208-20792227 MsG0780037158.01.T01:CDS 35.0%
GCAATGCAGTTGAAATTGAA+AGG - Chr7:20792104-20792123 MsG0780037158.01.T01:CDS 35.0%
TCGTTTGAAAAGGTTAATCC+TGG + Chr7:20791983-20792002 None:intergenic 35.0%
! ATGGAATTGATTTCGCTTCA+AGG - Chr7:20792271-20792290 MsG0780037158.01.T01:CDS 35.0%
! GGATGTTTTCAATTGCAATC+TGG + Chr7:20791721-20791740 None:intergenic 35.0%
! GTAGAAGGAATTTTCTTCTC+TGG - Chr7:20791914-20791933 MsG0780037158.01.T01:CDS 35.0%
! TGGAATTGATTTCGCTTCAA+GGG - Chr7:20792272-20792291 MsG0780037158.01.T01:CDS 35.0%
! TGTACATTTCCCAGATTTTG+GGG + Chr7:20791490-20791509 None:intergenic 35.0%
!!! AGATTTTGGGGTATTGGATT+GGG + Chr7:20791478-20791497 None:intergenic 35.0%
AAAAAACGCGGTTTAATGCC+AGG - Chr7:20791962-20791981 MsG0780037158.01.T01:CDS 40.0%
CAATCCAATACCCCAAAATC+TGG - Chr7:20791477-20791496 MsG0780037158.01.T01:CDS 40.0%
GTTGCATTTGCTTGTGTAGA+AGG - Chr7:20791899-20791918 MsG0780037158.01.T01:CDS 40.0%
TATCGCGAAAAAGGCTGATT+GGG - Chr7:20791832-20791851 MsG0780037158.01.T01:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG - Chr7:20792252-20792271 MsG0780037158.01.T01:CDS 40.0%
TGCAATCTGGAAACCGTAAA+AGG + Chr7:20791708-20791727 None:intergenic 40.0%
! AATTTCTTTGAGAAGCGCGT+TGG - Chr7:20792301-20792320 MsG0780037158.01.T01:CDS 40.0%
! ATTTCTTTGAGAAGCGCGTT+GGG - Chr7:20792302-20792321 MsG0780037158.01.T01:CDS 40.0%
!!! CAGATTTTGGGGTATTGGAT+TGG + Chr7:20791479-20791498 None:intergenic 40.0%
!!! TTTCCCAGATTTTGGGGTAT+TGG + Chr7:20791484-20791503 None:intergenic 40.0%
AAAAAGCCGAAGCCTCATTC+TGG - Chr7:20791510-20791529 MsG0780037158.01.T01:CDS 45.0%
ACTAACTTCAGAGCGTGTGA+AGG - Chr7:20792072-20792091 MsG0780037158.01.T01:CDS 45.0%
AGGCTGATTGGGCAATGAAA+TGG - Chr7:20791843-20791862 MsG0780037158.01.T01:CDS 45.0%
ATTGTCCTCGAAAATCTCGC+TGG - Chr7:20791644-20791663 MsG0780037158.01.T01:CDS 45.0%
GGGTGAACTTGGTTGTGAAA+AGG - Chr7:20792219-20792238 MsG0780037158.01.T01:CDS 45.0%
GTATCGCGAAAAAGGCTGAT+TGG - Chr7:20791831-20791850 MsG0780037158.01.T01:CDS 45.0%
GTTGTTCCAGTGCTTGAGAT+CGG + Chr7:20791561-20791580 None:intergenic 45.0%
! ATCTTCCAGCGAGATTTTCG+AGG + Chr7:20791652-20791671 None:intergenic 45.0%
! GGAGGAAAGATCGACTTCTT+CGG + Chr7:20791540-20791559 None:intergenic 45.0%
! TTTCTTTGAGAAGCGCGTTG+GGG - Chr7:20792303-20792322 MsG0780037158.01.T01:CDS 45.0%
!! TCAGCCTTTTTCGCGATACA+TGG + Chr7:20791830-20791849 None:intergenic 45.0%
CACTGGAACAACCTCACAGA+CGG - Chr7:20791569-20791588 MsG0780037158.01.T01:CDS 50.0%
GAGCTTATTTCGCGTGACGA+AGG - Chr7:20792001-20792020 MsG0780037158.01.T01:CDS 50.0%
TTCATTCCAACCAACGCGCA+AGG + Chr7:20792151-20792170 None:intergenic 50.0%
TTCGAGGACAATTCCGTCTG+AGG + Chr7:20791636-20791655 None:intergenic 50.0%
TTTCCTCCGATCTCAAGCAC+TGG - Chr7:20791552-20791571 MsG0780037158.01.T01:CDS 50.0%
! ACCGTAAAAGGCACGTGCTT+CGG + Chr7:20791696-20791715 None:intergenic 50.0%
! GAAGCTTCTGCGCGATTTTC+TGG - Chr7:20791936-20791955 MsG0780037158.01.T01:CDS 50.0%
! GGAAAGATCGACTTCTTCGG+CGG + Chr7:20791537-20791556 None:intergenic 50.0%
! TCCGAAGCACGTGCCTTTTA+CGG - Chr7:20791692-20791711 MsG0780037158.01.T01:CDS 50.0%
!! GCGTTGGTTGGAATGAATGG+TGG - Chr7:20792154-20792173 MsG0780037158.01.T01:CDS 50.0%
!! TGCGCGTTGGTTGGAATGAA+TGG - Chr7:20792151-20792170 MsG0780037158.01.T01:CDS 50.0%
CGAGGACGTGTGAGATGAAG+TGG + Chr7:20791601-20791620 None:intergenic 55.0%
GAGGACAATTCCGTCTGAGG+CGG + Chr7:20791633-20791652 None:intergenic 55.0%
GTTCCAGTGCTTGAGATCGG+AGG + Chr7:20791558-20791577 None:intergenic 55.0%
TTCTTCGGCGGTCCAGAATG+AGG + Chr7:20791525-20791544 None:intergenic 55.0%
GGCGGTCCAGAATGAGGCTT+CGG + Chr7:20791519-20791538 None:intergenic 60.0%
AGTGGCGTTCGCCGTCTGTG+AGG + Chr7:20791583-20791602 None:intergenic 65.0%
GCCTTCTTCGCCGCCTCAGA+CGG - Chr7:20791620-20791639 MsG0780037158.01.T01:CDS 65.0%
! GCGCTTCCTTGCGCGTTGGT+TGG - Chr7:20792142-20792161 MsG0780037158.01.T01:CDS 65.0%
! TCCGTCTGAGGCGGCGAAGA+AGG + Chr7:20791624-20791643 None:intergenic 65.0%
CGACGCGCTTCCTTGCGCGT+TGG - Chr7:20792138-20792157 MsG0780037158.01.T01:CDS 70.0%
CTGAGGCGGCGAAGAAGGCG+AGG + Chr7:20791619-20791638 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 20791470 20792408 20791470 ID=MsG0780037158.01;Name=MsG0780037158.01
Chr7 mRNA 20791470 20792408 20791470 ID=MsG0780037158.01.T01;Parent=MsG0780037158.01;Name=MsG0780037158.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|312
Chr7 exon 20791470 20792408 20791470 ID=MsG0780037158.01.T01:exon:5142;Parent=MsG0780037158.01.T01
Chr7 CDS 20791470 20792408 20791470 ID=MsG0780037158.01.T01:cds;Parent=MsG0780037158.01.T01
Gene Sequence

>MsG0780037158.01.T01

ATGTTCCCAATCCAATACCCCAAAATCTGGGAAATGTACAAAAAAGCCGAAGCCTCATTCTGGACCGCCGAAGAAGTCGATCTTTCCTCCGATCTCAAGCACTGGAACAACCTCACAGACGGCGAACGCCACTTCATCTCACACGTCCTCGCCTTCTTCGCCGCCTCAGACGGAATTGTCCTCGAAAATCTCGCTGGAAGATTCATGAAAGAAGTTCAAGTCTCCGAAGCACGTGCCTTTTACGGTTTCCAGATTGCAATTGAAAACATCCATTCTGAGATGTATTCACTTCTCCTTGAAACGTATATAAGCGATTCTGCTGAAAAGAATCGCTTATTTCACGCAATTGAAACAATTCCATGTATCGCGAAAAAGGCTGATTGGGCAATGAAATGGATTGATTCAAGCGATTCTTTTGCAGAAAGAATCGTTGCATTTGCTTGTGTAGAAGGAATTTTCTTCTCTGGAAGCTTCTGCGCGATTTTCTGGTTGAAAAAACGCGGTTTAATGCCAGGATTAACCTTTTCAAACGAGCTTATTTCGCGTGACGAAGGTTTACATTGTGATTTCGCTTGTTTGTTATACTCACTTCTACGCAAGAAACTAACTTCAGAGCGTGTGAAGGAAATTGTATGCAATGCAGTTGAAATTGAAAGGGAATTTGTCTGCGACGCGCTTCCTTGCGCGTTGGTTGGAATGAATGGTGGATTGATGAGTACTTATATTGAATTTGTTGCTGATAGATTATTGGGTGAACTTGGTTGTGAAAAGGTTTATAATGTTCAGAATCCGTTTGATTGGATGGAATTGATTTCGCTTCAAGGGAAGACGAATTTCTTTGAGAAGCGCGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATAGCTTGAATGGGAATGGTGCTGCTGATTTTTGCTTTAGTTTGGATGAAGATTTTTAG

Protein sequence

>MsG0780037158.01.T01

MFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLKHWNNLTDGERHFISHVLAFFAASDGIVLENLAGRFMKEVQVSEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIETIPCIAKKADWAMKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLTSERVKEIVCNAVEIEREFVCDALPCALVGMNGGLMSTYIEFVADRLLGELGCEKVYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMNSLNGNGAADFCFSLDEDF*