AlfalfaGEDB | MsG0580024657.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024657.01

KEGG: K15731
GO: GO:0016791
PFAM: PF03031

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024657.01.T01	XP_003593807.1	97.619	294	7	0	1	294	1	294	0	585

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024657.01.T01	P58465	46.626	163	86	1	106	268	105	266	1.97E-37	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024657.01.T01	G7ILM8	97.619	294	7	0	1	294	1	294	0.0	585

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048258.01	MsG0580024657.01	0.820959	5.206290e-53	4.179967e-50
MsG0080048311.01	MsG0580024657.01	0.800380	1.481196e-48	6.886851e-46
MsG0080048775.01	MsG0580024657.01	0.838401	2.938778e-57	3.931793e-54
MsG0080048781.01	MsG0580024657.01	0.861382	1.066849e-63	3.013271e-60
MsG0080049140.01	MsG0580024657.01	0.814272	1.676134e-51	1.119650e-48
MsG0180001293.01	MsG0580024657.01	0.822164	2.742522e-53	2.277520e-50
MsG0180002218.01	MsG0580024657.01	0.811880	5.610262e-51	3.513569e-48
MsG0180002397.01	MsG0580024657.01	0.848285	6.756378e-60	1.233841e-56
MsG0180002421.01	MsG0580024657.01	0.801381	9.249794e-49	4.410987e-46
MsG0180002429.01	MsG0580024657.01	0.806828	6.805984e-50	3.731247e-47
MsG0180002583.01	MsG0580024657.01	0.821109	4.807118e-53	3.875435e-50
MsG0180002984.01	MsG0580024657.01	0.811193	7.911789e-51	4.865907e-48
MsG0180003427.01	MsG0580024657.01	0.817610	3.015655e-52	2.206540e-49
MsG0180003485.01	MsG0580024657.01	0.811703	6.131672e-51	3.822046e-48
MsG0180003658.01	MsG0580024657.01	0.854978	8.584288e-62	1.951693e-58
MsG0180003698.01	MsG0580024657.01	0.822619	2.149641e-53	1.808764e-50
MsG0180003796.01	MsG0580024657.01	0.849908	2.390716e-60	4.602652e-57
MsG0180003857.01	MsG0580024657.01	0.843496	1.351760e-58	2.118804e-55
MsG0180004027.01	MsG0580024657.01	0.804902	1.727986e-49	9.017876e-47
MsG0180004120.01	MsG0580024657.01	0.806240	9.057124e-50	4.891371e-47
MsG0180004126.01	MsG0580024657.01	0.891296	4.522415e-74	3.942023e-70
MsG0180004185.01	MsG0580024657.01	0.829231	5.778034e-55	5.878271e-52
MsG0180004299.01	MsG0580024657.01	0.844556	7.024992e-59	1.138441e-55
MsG0180004538.01	MsG0580024657.01	0.823388	1.422776e-53	1.223203e-50
MsG0180004583.01	MsG0580024657.01	0.822256	2.610411e-53	2.173595e-50
MsG0180004806.01	MsG0580024657.01	0.805700	1.175971e-49	6.263992e-47
MsG0180004995.01	MsG0580024657.01	0.834115	3.611590e-56	4.244662e-53
MsG0180005002.01	MsG0580024657.01	0.836162	1.099757e-56	1.374552e-53
MsG0180005048.01	MsG0580024657.01	0.841760	3.909133e-58	5.801644e-55
MsG0180005435.01	MsG0580024657.01	0.815732	7.952782e-52	5.527754e-49
MsG0180005835.01	MsG0580024657.01	0.819765	9.779341e-53	7.594858e-50
MsG0180006133.01	MsG0580024657.01	0.861480	9.959548e-64	2.822179e-60
MsG0280010579.01	MsG0580024657.01	0.822726	2.030208e-53	1.713339e-50
MsG0280011154.01	MsG0580024657.01	0.817088	3.953003e-52	2.851307e-49
MsG0280011331.01	MsG0580024657.01	0.826648	2.417501e-54	2.280722e-51
MsG0380012788.01	MsG0580024657.01	0.801008	1.102603e-48	5.209182e-46
MsG0380013041.01	MsG0580024657.01	0.822396	2.422583e-53	2.025443e-50
MsG0380013584.01	MsG0580024657.01	0.801381	9.249040e-49	4.410644e-46
MsG0380014666.01	MsG0580024657.01	0.811097	8.300146e-51	5.091952e-48
MsG0480018526.01	MsG0580024657.01	0.861268	1.155850e-63	3.251843e-60
MsG0480018785.01	MsG0580024657.01	0.836032	1.186775e-56	1.477360e-53
MsG0480018885.01	MsG0580024657.01	0.813333	2.698747e-51	1.757377e-48
MsG0480020856.01	MsG0580024657.01	0.816583	5.132108e-52	3.650913e-49
MsG0480021016.01	MsG0580024657.01	0.811823	5.772880e-51	3.609962e-48
MsG0480021301.01	MsG0580024657.01	0.809769	1.606689e-50	9.517110e-48
MsG0480022320.01	MsG0580024657.01	0.801542	8.572986e-49	4.104847e-46
MsG0480022559.01	MsG0580024657.01	0.836424	9.433679e-57	1.188383e-53
MsG0480022784.01	MsG0580024657.01	0.836941	6.964902e-57	8.911420e-54
MsG0480022982.01	MsG0580024657.01	0.811977	5.345402e-51	3.356478e-48
MsG0480022991.01	MsG0580024657.01	0.807836	4.160184e-50	2.341288e-47
MsG0480023003.01	MsG0580024657.01	0.813104	3.030325e-51	1.960863e-48
MsG0480023064.01	MsG0580024657.01	0.815989	6.966458e-52	4.876124e-49
MsG0480023194.01	MsG0580024657.01	0.839402	1.618654e-57	2.233016e-54
MsG0480023262.01	MsG0580024657.01	0.811382	7.200433e-51	4.450445e-48
MsG0480023296.01	MsG0580024657.01	0.863267	2.812929e-64	8.477551e-61
MsG0480023484.01	MsG0580024657.01	0.808250	3.396898e-50	1.932780e-47
MsG0480023653.01	MsG0580024657.01	0.829196	5.894618e-55	5.990791e-52
MsG0480023672.01	MsG0580024657.01	0.855051	8.177429e-62	1.863710e-58
MsG0480023706.01	MsG0580024657.01	0.831611	1.513492e-55	1.650928e-52
MsG0480024002.01	MsG0580024657.01	0.818199	2.220603e-52	1.651257e-49
MsG0580024657.01	MsG0580024742.01	0.801944	7.088323e-49	3.428713e-46
MsG0580024657.01	MsG0580024787.01	0.879041	1.721337e-69	9.194502e-66
MsG0580024657.01	MsG0580024813.01	0.844568	6.973664e-59	1.130560e-55
MsG0580024657.01	MsG0580024954.01	0.844177	8.884374e-59	1.422669e-55
MsG0580024657.01	MsG0580025093.01	0.857752	1.317235e-62	3.285525e-59
MsG0580024657.01	MsG0580025177.01	0.832388	9.728026e-56	1.085703e-52
MsG0580024657.01	MsG0580025186.01	0.804951	1.688474e-49	8.822251e-47
MsG0580024657.01	MsG0580025464.01	0.803539	3.322077e-49	1.673850e-46
MsG0580024657.01	MsG0580025506.01	0.817041	4.050912e-52	2.918229e-49
MsG0580024657.01	MsG0580025515.01	0.804987	1.658734e-49	8.674634e-47
MsG0580024657.01	MsG0580025775.01	0.834671	2.619719e-56	3.130346e-53
MsG0580024657.01	MsG0580028756.01	0.846279	2.400586e-59	4.110450e-56
MsG0580024657.01	MsG0580028923.01	0.820717	5.917924e-53	4.718782e-50
MsG0580024657.01	MsG0580028927.01	0.812839	3.463404e-51	2.225313e-48
MsG0580024657.01	MsG0580029123.01	0.804168	2.458524e-49	1.258846e-46
MsG0580024657.01	MsG0580029197.01	0.801757	7.744310e-49	3.728199e-46
MsG0580024657.01	MsG0580029261.01	0.818593	1.807748e-52	1.358912e-49
MsG0580024657.01	MsG0580029268.01	0.808588	2.877639e-50	1.651741e-47
MsG0580024657.01	MsG0580029591.01	0.853778	1.908531e-61	4.170393e-58
MsG0580024657.01	MsG0680031616.01	0.877636	5.362172e-69	2.715548e-65
MsG0580024657.01	MsG0680032772.01	0.807429	5.077550e-50	2.827450e-47
MsG0580024657.01	MsG0680033405.01	0.817717	2.852608e-52	2.093390e-49
MsG0580024657.01	MsG0680033696.01	0.898177	6.849971e-77	8.007976e-73
MsG0580024657.01	MsG0680034651.01	0.806138	9.516714e-50	5.126060e-47
MsG0580024657.01	MsG0680034764.01	0.803901	2.793569e-49	1.420739e-46
MsG0580024657.01	MsG0680035350.01	0.805386	1.368987e-49	7.232774e-47
MsG0580024657.01	MsG0680035776.01	0.837510	4.983081e-57	6.487648e-54
MsG0580024657.01	MsG0680035878.01	0.812516	4.076524e-51	2.597306e-48
MsG0580024657.01	MsG0780036534.01	0.808748	2.658968e-50	1.532663e-47
MsG0580024657.01	MsG0780037741.01	0.809024	2.320726e-50	1.347544e-47
MsG0580024657.01	MsG0780038393.01	0.819481	1.135262e-52	8.747120e-50
MsG0580024657.01	MsG0780038402.01	0.822083	2.864334e-53	2.373090e-50
MsG0580024657.01	MsG0780038445.01	0.816917	4.318485e-52	3.100374e-49
MsG0580024657.01	MsG0780038736.01	0.803027	4.240070e-49	2.108312e-46
MsG0580024657.01	MsG0780039578.01	0.817566	3.086239e-52	2.255395e-49
MsG0580024657.01	MsG0780039861.01	0.864482	1.177847e-64	3.703771e-61
MsG0580024657.01	MsG0780040364.01	0.872742	2.517483e-67	1.065278e-63
MsG0580024657.01	MsG0780040564.01	0.804094	2.547360e-49	1.301906e-46
MsG0580024657.01	MsG0780040620.01	0.838955	2.114548e-57	2.877073e-54
MsG0580024657.01	MsG0780040641.01	0.818837	1.591478e-52	1.204534e-49
MsG0580024657.01	MsG0780040828.01	0.811532	6.677742e-51	4.143979e-48
MsG0580024657.01	MsG0780041132.01	0.817844	2.671199e-52	1.967128e-49
MsG0580024657.01	MsG0780041141.01	0.835930	1.259591e-56	1.563185e-53
MsG0580024657.01	MsG0780041142.01	0.843062	1.765431e-58	2.729308e-55
MsG0580024657.01	MsG0780041143.01	0.872287	3.571683e-67	1.486093e-63
MsG0580024657.01	MsG0780041340.01	0.845533	3.826964e-59	6.397069e-56
MsG0580024657.01	MsG0780041359.01	0.824252	8.925728e-54	7.863723e-51
MsG0580024657.01	MsG0880041930.01	0.805854	1.091795e-49	5.838577e-47
MsG0580024657.01	MsG0880042906.01	0.828160	1.048917e-54	1.034226e-51
MsG0580024657.01	MsG0880042927.01	0.832152	1.112925e-55	1.233343e-52
MsG0580024657.01	MsG0880042929.01	0.814201	1.737854e-51	1.158589e-48
MsG0580024657.01	MsG0880043714.01	0.803538	3.324066e-49	1.674793e-46
MsG0580024657.01	MsG0880043752.01	0.802517	5.404544e-49	2.652628e-46
MsG0580024657.01	MsG0880044103.01	0.809706	1.657511e-50	9.801369e-48
MsG0580024657.01	MsG0880045439.01	0.809450	1.881171e-50	1.104812e-47
MsG0580024657.01	MsG0880045584.01	0.806973	6.341869e-50	3.489838e-47
MsG0580024657.01	MsG0880045612.01	0.847414	1.174231e-59	2.085487e-56
MsG0580024657.01	MsG0880045647.01	0.810334	1.213815e-50	7.296471e-48
MsG0580024657.01	MsG0880046431.01	0.824243	8.972689e-54	7.903089e-51
MsG0580024657.01	MsG0880046885.01	0.828294	9.738259e-55	9.638679e-52
MsG0580024657.01	MsG0880046904.01	0.830443	2.928445e-55	3.085623e-52
MsG0580024657.01	MsG0880046906.01	0.815126	1.084324e-51	7.411982e-49
MsG0580024657.01	MsG0880046991.01	0.813522	2.452677e-51	1.605399e-48
MsG0580024657.01	MsG0880047223.01	0.835393	1.722787e-56	2.103955e-53
MsG0580024657.01	MsG0880047226.01	0.824001	1.022614e-53	8.945313e-51
MsG0580024657.01	MsG0880047466.01	0.825968	3.510298e-54	3.248359e-51
MsG0580024657.01	MsG0880047640.01	0.815216	1.035912e-51	7.098087e-49
MsG0580024657.01	MsG0880047649.01	0.848444	6.105898e-60	1.120805e-56
MsG0380015219.01	MsG0580024657.01	0.840861	6.736569e-58	9.722869e-55
MsG0380015394.01	MsG0580024657.01	0.830399	3.002076e-55	3.159239e-52
MsG0380015816.01	MsG0580024657.01	0.813557	2.410298e-51	1.579121e-48
MsG0380016027.01	MsG0580024657.01	0.801283	9.685704e-49	4.607618e-46
MsG0380016091.01	MsG0580024657.01	0.840750	7.203758e-58	1.036115e-54
MsG0380016092.01	MsG0580024657.01	0.806659	7.386551e-50	4.031734e-47
MsG0380016217.01	MsG0580024657.01	0.844163	8.961518e-59	1.434336e-55
MsG0380016225.01	MsG0580024657.01	0.831565	1.553501e-55	1.692261e-52
MsG0380017164.01	MsG0580024657.01	0.823532	1.316984e-53	1.136949e-50
MsG0380017240.01	MsG0580024657.01	0.804191	2.431679e-49	1.245815e-46
MsG0380017332.01	MsG0580024657.01	0.807260	5.514023e-50	3.057067e-47
MsG0380017361.01	MsG0580024657.01	0.853723	1.979580e-61	4.317859e-58
MsG0380017605.01	MsG0580024657.01	0.813857	2.070287e-51	1.367391e-48
MsG0380017632.01	MsG0580024657.01	0.810705	1.009247e-50	6.127275e-48
MsG0280006540.01	MsG0580024657.01	0.849548	3.013110e-60	5.731860e-57
MsG0280006563.01	MsG0580024657.01	0.871073	9.020806e-67	3.591057e-63
MsG0280007003.01	MsG0580024657.01	0.816347	5.795112e-52	4.096315e-49
MsG0280007359.01	MsG0580024657.01	0.822043	2.925310e-53	2.420900e-50
MsG0280007419.01	MsG0580024657.01	0.827355	1.637877e-54	1.577601e-51
MsG0280007761.01	MsG0580024657.01	0.816602	5.080635e-52	3.616268e-49
MsG0280009752.01	MsG0580024657.01	0.806204	9.214821e-50	4.972057e-47
MsG0280009866.01	MsG0580024657.01	0.802662	5.043785e-49	2.484778e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024657.01.T01	MTR_2g017810	97.619	294	7	0	1	294	1	294	0.0	585
MsG0580024657.01.T01	MTR_5g045920	37.714	175	103	2	101	274	123	292	2.07e-28	111
MsG0580024657.01.T01	MTR_3g006690	39.572	187	105	3	90	269	272	457	1.40e-26	108
MsG0580024657.01.T01	MTR_2g049420	36.325	234	138	5	54	280	103	332	2.43e-26	106
MsG0580024657.01.T01	MTR_2g049420	36.325	234	138	5	54	280	240	469	6.30e-26	107
MsG0580024657.01.T01	MTR_2g049420	36.325	234	138	5	54	280	231	460	7.39e-26	106
MsG0580024657.01.T01	MTR_3g085400	40.972	144	83	2	109	251	323	465	2.12e-25	105
MsG0580024657.01.T01	MTR_3g085395	39.583	144	85	2	109	251	201	343	5.15e-23	97.8
MsG0580024657.01.T01	MTR_3g070810	38.217	157	78	4	106	243	70	226	3.65e-20	88.2
MsG0580024657.01.T01	MTR_5g082660	37.580	157	79	4	106	243	96	252	6.43e-17	79.7
MsG0580024657.01.T01	MTR_5g095530	31.210	157	89	2	91	244	172	312	1.27e-15	76.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024657.01.T01	AT5G45700	43.463	283	146	6	1	280	1	272	2.02e-62	199
MsG0580024657.01.T01	AT1G29780	55.814	172	75	1	107	277	49	220	6.81e-59	188
MsG0580024657.01.T01	AT1G29770	52.571	175	81	2	107	279	102	276	3.53e-57	185
MsG0580024657.01.T01	AT5G11860	37.576	165	101	2	109	272	113	276	3.33e-27	108
MsG0580024657.01.T01	AT5G11860	37.576	165	101	2	109	272	113	276	3.33e-27	108
MsG0580024657.01.T01	AT5G11860	37.576	165	101	2	109	272	113	276	3.33e-27	108
MsG0580024657.01.T01	AT5G11860	37.576	165	101	2	109	272	113	276	3.33e-27	108
MsG0580024657.01.T01	AT5G11860	37.576	165	101	2	109	272	113	276	3.33e-27	108
MsG0580024657.01.T01	AT5G46410	39.884	173	102	2	109	280	285	456	3.84e-25	104
MsG0580024657.01.T01	AT5G46410	39.884	173	102	2	109	280	282	453	4.66e-25	104
MsG0580024657.01.T01	AT5G46410	39.884	173	102	2	109	280	282	453	4.66e-25	104
MsG0580024657.01.T01	AT4G18140	39.884	173	102	2	109	280	270	441	1.58e-23	100
MsG0580024657.01.T01	AT4G18140	39.884	173	102	2	109	280	270	441	1.58e-23	100
MsG0580024657.01.T01	AT4G18140	39.884	173	102	2	109	280	270	441	1.58e-23	100
MsG0580024657.01.T01	AT4G18140	40.606	165	96	2	109	272	270	433	4.97e-23	98.6
MsG0580024657.01.T01	AT4G18140	40.606	165	96	2	109	272	270	433	4.97e-23	98.6
MsG0580024657.01.T01	AT4G18140	40.606	165	96	2	109	272	270	433	6.17e-23	98.2
MsG0580024657.01.T01	AT1G55900	31.792	173	99	2	91	260	171	327	4.51e-19	86.7
MsG0580024657.01.T01	AT1G55900	31.792	173	99	2	91	260	171	327	4.97e-19	86.7
MsG0580024657.01.T01	AT3G55960	36.184	152	78	4	106	238	96	247	2.52e-16	78.2

Find 61 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGAAGCTTGTGAAGGATT+TGG	0.246705	5:+7809661	MsG0580024657.01.T01:CDS
TTCAAAGCTTCTCACAAAAC+TGG	0.265788	5:+7809190	MsG0580024657.01.T01:CDS
GGGAAGCTTGTGAAGGATTT+GGG	0.271030	5:+7809662	MsG0580024657.01.T01:CDS
TTATTTCGCATCGGCTTTAT+AGG	0.299291	5:+7809618	MsG0580024657.01.T01:CDS
GCTTAAGGAGTATGCGAGTT+TGG	0.339130	5:+7809568	MsG0580024657.01.T01:CDS
GGTTGATTCGAAAATGAAAC+TGG	0.340128	5:-7809731	None:intergenic
GTTAATGTCTTTGATTGATT+TGG	0.348380	5:-7809084	None:intergenic
TTCCACCTTCAATATCTAAT+CGG	0.378188	5:+7809351	MsG0580024657.01.T01:CDS
TATTTCGCATCGGCTTTATA+GGG	0.378274	5:+7809619	MsG0580024657.01.T01:CDS
CATCTGATCAAAGGCGTGTC+TGG	0.397775	5:+7809908	MsG0580024657.01.T01:CDS
GCCGCGACGACGTTCTTTAT+CGG	0.415564	5:-7809131	None:intergenic
TGGACCGGTTGGACCGGAAC+GGG	0.415871	5:+7809594	MsG0580024657.01.T01:CDS
TGTGGGTAGGGATTTGAAGA+AGG	0.416733	5:+7809688	MsG0580024657.01.T01:CDS
ATGCTTGTGATCGTGAGCTT+TGG	0.419497	5:+7809789	MsG0580024657.01.T01:CDS
TTAATCAAAATCGCATTCTC+AGG	0.423966	5:-7809752	None:intergenic
GTAGTCCGGCCGGTGATTGA+TGG	0.425963	5:+7809449	MsG0580024657.01.T01:CDS
CTCGTCTCAGTTCTTCAAAT+CGG	0.429741	5:+7809219	MsG0580024657.01.T01:CDS
TTTGAAGAAGGTTGTTATTG+TGG	0.430715	5:+7809700	MsG0580024657.01.T01:CDS
TCTTGATTTAGATGAAACTT+TGG	0.436405	5:+7809385	MsG0580024657.01.T01:CDS
TAAACCCGTTCCGGTCCAAC+CGG	0.468555	5:-7809598	None:intergenic
GATGCGAAATAAACCCGTTC+CGG	0.471268	5:-7809607	None:intergenic
ACCGGGGATTGATGAATTGT+TGG	0.482144	5:+7809502	MsG0580024657.01.T01:CDS
GTGGACCGGTTGGACCGGAA+CGG	0.486922	5:+7809593	MsG0580024657.01.T01:CDS
CTTTGAAATGCGGCGGTGGA+TGG	0.490657	5:-7809174	None:intergenic
GGAGTATGCGAGTTTGGTTG+TGG	0.507228	5:+7809574	MsG0580024657.01.T01:CDS
TTCTACGTGTTGAAGCGACC+GGG	0.513582	5:+7809485	MsG0580024657.01.T01:CDS
TAGGGATTCGTGTAGGAATG+TGG	0.519949	5:+7809637	MsG0580024657.01.T01:CDS
AAATCAAATTTCTCCGGCGG+TGG	0.522620	5:-7809425	None:intergenic
TCACAAGCATCATCAACAAA+AGG	0.529060	5:-7809776	None:intergenic
AGCGTTGAAATATGAGGTGG+TGG	0.529684	5:+7809532	MsG0580024657.01.T01:CDS
CGTGAGCTTTGGAAATTGAG+AGG	0.530120	5:+7809800	MsG0580024657.01.T01:CDS
TTTCTACGTGTTGAAGCGAC+CGG	0.530989	5:+7809484	MsG0580024657.01.T01:CDS
TGGTTGTGGACCGGTTGGAC+CGG	0.534625	5:+7809588	MsG0580024657.01.T01:CDS
ATGCGAGTTTGGTTGTGGAC+CGG	0.538407	5:+7809579	MsG0580024657.01.T01:CDS
TCAAAGCTTCTCACAAAACT+GGG	0.544321	5:+7809191	MsG0580024657.01.T01:CDS
ACTACAAAATCAAATTTCTC+CGG	0.568154	5:-7809431	None:intergenic
GGAACGGGTTTATTTCGCAT+CGG	0.571199	5:+7809609	MsG0580024657.01.T01:CDS
GATTCGTGTAGGAATGTGGA+AGG	0.572672	5:+7809641	MsG0580024657.01.T01:CDS
AAGACATTAACCGTCGTCGT+TGG	0.574857	5:+7809096	MsG0580024657.01.T01:CDS
TCGTGTTCACATCTGATCAA+AGG	0.577857	5:+7809899	MsG0580024657.01.T01:CDS
GAAGCTTTGAAATGCGGCGG+TGG	0.579230	5:-7809178	None:intergenic
TTGTGAGAAGCTTTGAAATG+CGG	0.588582	5:-7809184	None:intergenic
ATCGGTTCACCATCAATCAC+CGG	0.589525	5:-7809458	None:intergenic
TCCAACAATTCATCAATCCC+CGG	0.590132	5:-7809503	None:intergenic
TGTGGAAGGGAAGCTTGTGA+AGG	0.595546	5:+7809655	MsG0580024657.01.T01:CDS
TCTACGTGTTGAAGCGACCG+GGG	0.598046	5:+7809486	MsG0580024657.01.T01:CDS
GATGGAAGAAAGAACGGCGG+AGG	0.608748	5:-7809156	None:intergenic
TCCGATAAAGAACGTCGTCG+CGG	0.612729	5:+7809130	MsG0580024657.01.T01:CDS
TTCAACACGTAGAAATCCAT+CGG	0.617017	5:-7809476	None:intergenic
GGTGATTGATGGTGAACCGA+TGG	0.621208	5:+7809460	MsG0580024657.01.T01:CDS
GGCTTTATAGGGATTCGTGT+AGG	0.622796	5:+7809630	MsG0580024657.01.T01:CDS
GCGGTGGATGGAAGAAAGAA+CGG	0.625468	5:-7809162	None:intergenic
TTCCGATTAGATATTGAAGG+TGG	0.632258	5:-7809353	None:intergenic
AGCGTTAGCGTTGAAATATG+AGG	0.636943	5:+7809526	MsG0580024657.01.T01:CDS
GTTCACCATCAATCACCGGC+CGG	0.662293	5:-7809454	None:intergenic
TGAGAAGCTTTGAAATGCGG+CGG	0.672239	5:-7809181	None:intergenic
ACAAAATCAAATTTCTCCGG+CGG	0.674025	5:-7809428	None:intergenic
ATTCGTGTAGGAATGTGGAA+GGG	0.678685	5:+7809642	MsG0580024657.01.T01:CDS
GAGTTTGGTTGTGGACCGGT+TGG	0.681067	5:+7809583	MsG0580024657.01.T01:CDS
GTTAGCGTTGAAATATGAGG+TGG	0.719947	5:+7809529	MsG0580024657.01.T01:CDS
GTGGATGGAAGAAAGAACGG+CGG	0.776465	5:-7809159	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCATCTAAATCAAGAAAAA+CGG	-	Chr5:7809381-7809400	None:intergenic	20.0%
!	ACTACAAAATCAAATTTCTC+CGG	-	Chr5:7809434-7809453	None:intergenic	25.0%
!	GTTAATGTCTTTGATTGATT+TGG	-	Chr5:7809087-7809106	None:intergenic	25.0%
!	TCTTGATTTAGATGAAACTT+TGG	+	Chr5:7809385-7809404	MsG0580024657.01.T01:CDS	25.0%
!!!	AGAAATTTGATTTTGTAGTC+CGG	+	Chr5:7809435-7809454	MsG0580024657.01.T01:CDS	25.0%
!!!	TGATTGTTTTGATGATATGA+GGG	+	Chr5:7809838-7809857	MsG0580024657.01.T01:CDS	25.0%
	TTAATCAAAATCGCATTCTC+AGG	-	Chr5:7809755-7809774	None:intergenic	30.0%
	TTCCACCTTCAATATCTAAT+CGG	+	Chr5:7809351-7809370	MsG0580024657.01.T01:CDS	30.0%
!	GTTTTCCGATTAGATATTGA+AGG	-	Chr5:7809359-7809378	None:intergenic	30.0%
!	TTTGAAGAAGGTTGTTATTG+TGG	+	Chr5:7809700-7809719	MsG0580024657.01.T01:CDS	30.0%
!!!	AAATTGAGAGGCTTTTTTGA+TGG	+	Chr5:7809812-7809831	MsG0580024657.01.T01:CDS	30.0%
!!!	AATTGAGAGGCTTTTTTGAT+GGG	+	Chr5:7809813-7809832	MsG0580024657.01.T01:CDS	30.0%
!!!	GTGATTGTTTTGATGATATG+AGG	+	Chr5:7809837-7809856	MsG0580024657.01.T01:CDS	30.0%
	ACAAAATCAAATTTCTCCGG+CGG	-	Chr5:7809431-7809450	None:intergenic	35.0%
	TCAAAGCTTCTCACAAAACT+GGG	+	Chr5:7809191-7809210	MsG0580024657.01.T01:CDS	35.0%
	TCACAAGCATCATCAACAAA+AGG	-	Chr5:7809779-7809798	None:intergenic	35.0%
	TTCAAAGCTTCTCACAAAAC+TGG	+	Chr5:7809190-7809209	MsG0580024657.01.T01:CDS	35.0%
	TTCAACACGTAGAAATCCAT+CGG	-	Chr5:7809479-7809498	None:intergenic	35.0%
	TTCCGATTAGATATTGAAGG+TGG	-	Chr5:7809356-7809375	None:intergenic	35.0%
!	AAAAAGCAGGGTTTTGCAAA+CGG	-	Chr5:7809306-7809325	None:intergenic	35.0%
!!	ATATGAGGTGGTGGTTTTTA+CGG	+	Chr5:7809541-7809560	MsG0580024657.01.T01:CDS	35.0%
!!	GGTTGATTCGAAAATGAAAC+TGG	-	Chr5:7809734-7809753	None:intergenic	35.0%
!!	TATTTCGCATCGGCTTTATA+GGG	+	Chr5:7809619-7809638	MsG0580024657.01.T01:CDS	35.0%
!!	TTATTTCGCATCGGCTTTAT+AGG	+	Chr5:7809618-7809637	MsG0580024657.01.T01:CDS	35.0%
!!	TTGTGAGAAGCTTTGAAATG+CGG	-	Chr5:7809187-7809206	None:intergenic	35.0%
	ATTCGTGTAGGAATGTGGAA+GGG	+	Chr5:7809642-7809661	MsG0580024657.01.T01:CDS	40.0%
	CTCGTCTCAGTTCTTCAAAT+CGG	+	Chr5:7809219-7809238	MsG0580024657.01.T01:CDS	40.0%
	GACGCTATCTTCAAAAAGCA+GGG	-	Chr5:7809318-7809337	None:intergenic	40.0%
	TCCAACAATTCATCAATCCC+CGG	-	Chr5:7809506-7809525	None:intergenic	40.0%
	TCGTGTTCACATCTGATCAA+AGG	+	Chr5:7809899-7809918	MsG0580024657.01.T01:CDS	40.0%
!	AGCGTTAGCGTTGAAATATG+AGG	+	Chr5:7809526-7809545	MsG0580024657.01.T01:CDS	40.0%
!	GTTAGCGTTGAAATATGAGG+TGG	+	Chr5:7809529-7809548	MsG0580024657.01.T01:CDS	40.0%
!!	ATTTGATTTTGTAGTCCGGC+CGG	+	Chr5:7809439-7809458	MsG0580024657.01.T01:CDS	40.0%
!!	GTGAAGGATTTGGGTTTTGT+GGG	+	Chr5:7809671-7809690	MsG0580024657.01.T01:CDS	40.0%
!!	TGTGAAGGATTTGGGTTTTG+TGG	+	Chr5:7809670-7809689	MsG0580024657.01.T01:CDS	40.0%
	AAATCAAATTTCTCCGGCGG+TGG	-	Chr5:7809428-7809447	None:intergenic	45.0%
	AAGACATTAACCGTCGTCGT+TGG	+	Chr5:7809096-7809115	MsG0580024657.01.T01:CDS	45.0%
	ACCGGGGATTGATGAATTGT+TGG	+	Chr5:7809502-7809521	MsG0580024657.01.T01:CDS	45.0%
	AGGGAAGCTTGTGAAGGATT+TGG	+	Chr5:7809661-7809680	MsG0580024657.01.T01:CDS	45.0%
	ATGCTTGTGATCGTGAGCTT+TGG	+	Chr5:7809789-7809808	MsG0580024657.01.T01:CDS	45.0%
	CGACGCTATCTTCAAAAAGC+AGG	-	Chr5:7809319-7809338	None:intergenic	45.0%
	GATGCGAAATAAACCCGTTC+CGG	-	Chr5:7809610-7809629	None:intergenic	45.0%
	GATTCGTGTAGGAATGTGGA+AGG	+	Chr5:7809641-7809660	MsG0580024657.01.T01:CDS	45.0%
	GGGAAGCTTGTGAAGGATTT+GGG	+	Chr5:7809662-7809681	MsG0580024657.01.T01:CDS	45.0%
	TAGGGATTCGTGTAGGAATG+TGG	+	Chr5:7809637-7809656	MsG0580024657.01.T01:CDS	45.0%
	TGTGGGTAGGGATTTGAAGA+AGG	+	Chr5:7809688-7809707	MsG0580024657.01.T01:CDS	45.0%
	TTCAAAAACTTCACCACCGC+CGG	+	Chr5:7809412-7809431	MsG0580024657.01.T01:CDS	45.0%
	TTTCTACGTGTTGAAGCGAC+CGG	+	Chr5:7809484-7809503	MsG0580024657.01.T01:CDS	45.0%
!	AGCGTTGAAATATGAGGTGG+TGG	+	Chr5:7809532-7809551	MsG0580024657.01.T01:CDS	45.0%
!	ATCGGTTCACCATCAATCAC+CGG	-	Chr5:7809461-7809480	None:intergenic	45.0%
!	GCTTAAGGAGTATGCGAGTT+TGG	+	Chr5:7809568-7809587	MsG0580024657.01.T01:CDS	45.0%
!	GGAACGGGTTTATTTCGCAT+CGG	+	Chr5:7809609-7809628	MsG0580024657.01.T01:CDS	45.0%
!	GGCTTTATAGGGATTCGTGT+AGG	+	Chr5:7809630-7809649	MsG0580024657.01.T01:CDS	45.0%
!!	AGGATTTGGGTTTTGTGGGT+AGG	+	Chr5:7809675-7809694	MsG0580024657.01.T01:CDS	45.0%
!!	CGTGAGCTTTGGAAATTGAG+AGG	+	Chr5:7809800-7809819	MsG0580024657.01.T01:CDS	45.0%
!!	GGATTTGGGTTTTGTGGGTA+GGG	+	Chr5:7809676-7809695	MsG0580024657.01.T01:CDS	45.0%
!!	TGAGAAGCTTTGAAATGCGG+CGG	-	Chr5:7809184-7809203	None:intergenic	45.0%
	ATGCGAGTTTGGTTGTGGAC+CGG	+	Chr5:7809579-7809598	MsG0580024657.01.T01:CDS	50.0%
	GCGGTGGATGGAAGAAAGAA+CGG	-	Chr5:7809165-7809184	None:intergenic	50.0%
	GGAGTATGCGAGTTTGGTTG+TGG	+	Chr5:7809574-7809593	MsG0580024657.01.T01:CDS	50.0%
	GTGGATGGAAGAAAGAACGG+CGG	-	Chr5:7809162-7809181	None:intergenic	50.0%
	TCCGATAAAGAACGTCGTCG+CGG	+	Chr5:7809130-7809149	MsG0580024657.01.T01:CDS	50.0%
	TGTGGAAGGGAAGCTTGTGA+AGG	+	Chr5:7809655-7809674	MsG0580024657.01.T01:CDS	50.0%
	TTCTACGTGTTGAAGCGACC+GGG	+	Chr5:7809485-7809504	MsG0580024657.01.T01:CDS	50.0%
!	CATCTGATCAAAGGCGTGTC+TGG	+	Chr5:7809908-7809927	MsG0580024657.01.T01:CDS	50.0%
!!	GGTGATTGATGGTGAACCGA+TGG	+	Chr5:7809460-7809479	MsG0580024657.01.T01:CDS	50.0%
!!	TGAGGTGGTGGTTTTTACGG+CGG	+	Chr5:7809544-7809563	MsG0580024657.01.T01:CDS	50.0%
!!	TTTTTCGACGCCAACGACGA+CGG	-	Chr5:7809109-7809128	None:intergenic	50.0%
	GAGTTTGGTTGTGGACCGGT+TGG	+	Chr5:7809583-7809602	MsG0580024657.01.T01:CDS	55.0%
	GATGGAAGAAAGAACGGCGG+AGG	-	Chr5:7809159-7809178	None:intergenic	55.0%
	GCCGCGACGACGTTCTTTAT+CGG	-	Chr5:7809134-7809153	None:intergenic	55.0%
	GTTCACCATCAATCACCGGC+CGG	-	Chr5:7809457-7809476	None:intergenic	55.0%
	TAAACCCGTTCCGGTCCAAC+CGG	-	Chr5:7809601-7809620	None:intergenic	55.0%
	TCTACGTGTTGAAGCGACCG+GGG	+	Chr5:7809486-7809505	MsG0580024657.01.T01:CDS	55.0%
!	GGTTTTTACGGCGGCGCTTA+AGG	+	Chr5:7809553-7809572	MsG0580024657.01.T01:CDS	55.0%
!!	CTTTGAAATGCGGCGGTGGA+TGG	-	Chr5:7809177-7809196	None:intergenic	55.0%
!!	GAAGCTTTGAAATGCGGCGG+TGG	-	Chr5:7809181-7809200	None:intergenic	55.0%
	GTAGTCCGGCCGGTGATTGA+TGG	+	Chr5:7809449-7809468	MsG0580024657.01.T01:CDS	60.0%
	TGGTTGTGGACCGGTTGGAC+CGG	+	Chr5:7809588-7809607	MsG0580024657.01.T01:CDS	60.0%
	GTGGACCGGTTGGACCGGAA+CGG	+	Chr5:7809593-7809612	MsG0580024657.01.T01:CDS	65.0%
	TGGACCGGTTGGACCGGAAC+GGG	+	Chr5:7809594-7809613	MsG0580024657.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	7809053	7809937	7809053	ID=MsG0580024657.01;Name=MsG0580024657.01
Chr5	mRNA	7809053	7809937	7809053	ID=MsG0580024657.01.T01;Parent=MsG0580024657.01;Name=MsG0580024657.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|294
Chr5	exon	7809053	7809937	7809053	ID=MsG0580024657.01.T01:exon:19361;Parent=MsG0580024657.01.T01
Chr5	CDS	7809053	7809937	7809053	ID=MsG0580024657.01.T01:cds;Parent=MsG0580024657.01.T01

Gene Sequence

>MsG0580024657.01.T01

ATGGTTTCGAAGACAAAGAAGAAAGCTTCAACCAAATCAATCAAAGACATTAACCGTCGTCGTTGGCGTCGAAAAACTCCGATAAAGAACGTCGTCGCGGCTTCCTCCGCCGTTCTTTCTTCCATCCACCGCCGCATTTCAAAGCTTCTCACAAAACTGGGACTCACTCGTCTCAGTTCTTCAAATCGGAAACGAAACAGCTACAAAATTCTCAAGAAATCAACACATTTCAACTTTCAACAGCAAGACACCGTTTGCAAAACCCTGCTTTTTGAAGATAGCGTCGAAAATTCTCTTCTTCCACCTTCAATATCTAATCGGAAAACCGTTTTTCTTGATTTAGATGAAACTTTGGTTCATTCAAAAACTTCACCACCGCCGGAGAAATTTGATTTTGTAGTCCGGCCGGTGATTGATGGTGAACCGATGGATTTCTACGTGTTGAAGCGACCGGGGATTGATGAATTGTTGGAAGCGTTAGCGTTGAAATATGAGGTGGTGGTTTTTACGGCGGCGCTTAAGGAGTATGCGAGTTTGGTTGTGGACCGGTTGGACCGGAACGGGTTTATTTCGCATCGGCTTTATAGGGATTCGTGTAGGAATGTGGAAGGGAAGCTTGTGAAGGATTTGGGTTTTGTGGGTAGGGATTTGAAGAAGGTTGTTATTGTGGATGATAATCCAGTTTCATTTTCGAATCAACCTGAGAATGCGATTTTGATTAAGCCTTTTGTTGATGATGCTTGTGATCGTGAGCTTTGGAAATTGAGAGGCTTTTTTGATGGGTGTGATTGTTTTGATGATATGAGGGATGCTGTTAAACACTATGCTGCTGAGAAAGAACGTCGCTCGTGTTCACATCTGATCAAAGGCGTGTCTGGTGTCTAA

Protein sequence

>MsG0580024657.01.T01

MVSKTKKKASTKSIKDINRRRWRRKTPIKNVVAASSAVLSSIHRRISKLLTKLGLTRLSSSNRKRNSYKILKKSTHFNFQQQDTVCKTLLFEDSVENSLLPPSISNRKTVFLDLDETLVHSKTSPPPEKFDFVVRPVIDGEPMDFYVLKRPGIDELLEALALKYEVVVFTAALKEYASLVVDRLDRNGFISHRLYRDSCRNVEGKLVKDLGFVGRDLKKVVIVDDNPVSFSNQPENAILIKPFVDDACDRELWKLRGFFDGCDCFDDMRDAVKHYAAEKERRSCSHLIKGVSGV*