AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044835.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044835.01.T01 MTR_8g469010 99.023 307 3 0 1 307 19 325 0.0 629
MsG0880044835.01.T01 MTR_0268s0080 91.667 312 21 1 1 307 19 330 0.0 590
MsG0880044835.01.T01 MTR_1g050472 91.987 312 20 1 1 307 19 330 0.0 590
MsG0880044835.01.T01 MTR_7g029110 91.346 312 22 1 1 307 19 330 0.0 568
MsG0880044835.01.T01 MTR_4g117370 77.170 311 67 1 1 307 31 341 0.0 512
MsG0880044835.01.T01 MTR_6g005270 69.767 43 13 0 247 289 25 67 2.18e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044835.01.T01 AT3G23580 77.492 311 66 1 1 307 31 341 0.0 505
MsG0880044835.01.T01 AT3G27060 80.573 314 54 3 1 307 19 332 1.06e-179 499

Find 55 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGATTTGATTTCGCTTC+AGG 0.266722 8:-49939833 MsG0880044835.01.T01:CDS
TCCGAAGCGCGTGCTTTCTA+TGG 0.277078 8:-49940411 MsG0880044835.01.T01:CDS
CACTTCCTTGCGCGTTGGTT+GGG 0.285926 8:-49939960 MsG0880044835.01.T01:CDS
ATTTCTTTGAGAAGCGCGTT+GGG 0.337568 8:-49939801 MsG0880044835.01.T01:CDS
AAGCTGATTGGGCTTTGAAA+TGG 0.371966 8:-49940260 MsG0880044835.01.T01:CDS
TGCTGATTATTGCTTTACTT+TGG 0.386701 8:-49939746 MsG0880044835.01.T01:CDS
AATCGCTTATATACGTTTCA+AGG 0.387103 8:+49940340 None:intergenic
GCACTTCCTTGCGCGTTGGT+TGG 0.390027 8:-49939961 MsG0880044835.01.T01:CDS
ATGTTCAGAATCCGTTTGAT+TGG 0.390491 8:-49939855 MsG0880044835.01.T01:CDS
GAAAATCGCGCAGAAGCTTC+CGG 0.409737 8:+49940170 None:intergenic
GTTTGTTGCTGATAGGTTGT+TGG 0.410068 8:-49939905 MsG0880044835.01.T01:CDS
ACCATAGAAAGCACGCGCTT+CGG 0.432842 8:+49940410 None:intergenic
TCAGAATCCGTTTGATTGGA+TGG 0.443597 8:-49939851 MsG0880044835.01.T01:CDS
GATAGGTTGTTGGGTGAACT+TGG 0.452257 8:-49939895 MsG0880044835.01.T01:CDS
TTTGTTGCTGATAGGTTGTT+GGG 0.461848 8:-49939904 MsG0880044835.01.T01:CDS
TACTTCTTTCATGAATCTCC+CGG 0.464331 8:+49940440 None:intergenic
TATTGCTAAGAAAGCTGATT+GGG 0.465189 8:-49940271 MsG0880044835.01.T01:CDS
GCCTTCTTCGCCGCTTCCGA+CGG 0.470916 8:-49940483 MsG0880044835.01.T01:CDS
ACCGTCGGAAGCGGCGAAGA+AGG 0.474533 8:+49940482 None:intergenic
ATCAAATCCATCCAATCAAA+CGG 0.489030 8:+49939844 None:intergenic
ATGGATTTGATTTCGCTTCA+GGG 0.493555 8:-49939832 MsG0880044835.01.T01:CDS
TTCTTCAGCGGTCCAGAAAG+AGG 0.494376 8:+49940581 None:intergenic
AGCGGTCCAGAAAGAGGCTT+CGG 0.495985 8:+49940587 None:intergenic
CGACGCACTTCCTTGCGCGT+TGG 0.502621 8:-49939965 MsG0880044835.01.T01:CDS
TTATTGCTTTACTTTGGATG+AGG 0.510830 8:-49939740 MsG0880044835.01.T01:CDS
TTTCCTCCGATCTTCAACAC+TGG 0.515193 8:-49940551 MsG0880044835.01.T01:CDS
GTATTGCTAAGAAAGCTGAT+TGG 0.515724 8:-49940272 MsG0880044835.01.T01:CDS
TGCGCGTTGGTTGGGATGAA+CGG 0.518015 8:-49939952 MsG0880044835.01.T01:CDS
CAGAAAGCTTCTGTTATGAA+TGG 0.519026 8:-49939772 MsG0880044835.01.T01:CDS
GTGATGCAGTTGAGATTGAA+AGG 0.522321 8:-49939999 MsG0880044835.01.T01:CDS
AATTTCTTTGAGAAGCGCGT+TGG 0.523856 8:-49939802 MsG0880044835.01.T01:CDS
ATCGTCCTCGAAAACCTCGC+CGG 0.534897 8:-49940459 MsG0880044835.01.T01:CDS
ATATTGAGTTTGTTGCTGAT+AGG 0.535233 8:-49939912 MsG0880044835.01.T01:CDS
TCGTCCTCGAAAACCTCGCC+GGG 0.543275 8:-49940458 MsG0880044835.01.T01:CDS
GTTGTTCCAGTGTTGAAGAT+CGG 0.557909 8:+49940545 None:intergenic
GACGAAGTGGCGTTCGCCGT+CGG 0.563379 8:+49940518 None:intergenic
GAGGACGATACCGTCGGAAG+CGG 0.563447 8:+49940473 None:intergenic
TGATGCAGTTGAGATTGAAA+GGG 0.582117 8:-49939998 MsG0880044835.01.T01:CDS
TTCATCCCAACCAACGCGCA+AGG 0.597180 8:+49939955 None:intergenic
TTTCTTTGAGAAGCGCGTTG+GGG 0.598671 8:-49939800 MsG0880044835.01.T01:CDS
CGGAAGCGGCGAAGAAGGCG+AGG 0.605540 8:+49940487 None:intergenic
CTTTCATGAATCTCCCGGCG+AGG 0.609684 8:+49940445 None:intergenic
GAGCTTATTTCGTGTGACGA+AGG 0.610492 8:-49940102 MsG0880044835.01.T01:CDS
GAAGCTGAGTTCAGAGCGAG+TGG 0.611132 8:-49940034 MsG0880044835.01.T01:CDS
CACTGGAACAACCTCACCGA+CGG 0.611309 8:-49940534 MsG0880044835.01.T01:CDS
TCATTTGAGAAAGTTAATCC+CGG 0.611975 8:+49940123 None:intergenic
AGTGGCGTTCGCCGTCGGTG+AGG 0.618653 8:+49940523 None:intergenic
TGGATTTGATTTCGCTTCAG+GGG 0.620413 8:-49939831 MsG0880044835.01.T01:CDS
GTTCCAGTGTTGAAGATCGG+AGG 0.622372 8:+49940548 None:intergenic
CGAGGATGTGAGAGACGAAG+TGG 0.630959 8:+49940505 None:intergenic
TCAGCTTTCTTAGCAATACA+CGG 0.642160 8:+49940276 None:intergenic
AAGGAGAAGAGAATACATCT+CGG 0.671542 8:+49940359 None:intergenic
AAAGCTTCTGTTATGAATGG+TGG 0.674687 8:-49939769 MsG0880044835.01.T01:CDS
GGAAAGATCGACTTCTTCAG+CGG 0.689628 8:+49940569 None:intergenic
GAAGAGAATACATCTCGGAG+TGG 0.695258 8:+49940364 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTCAATACCCAAAAATCT+GGG - Chr8:49939740-49939759 MsG0880044835.01.T01:CDS 25.0%
!!! AGATTTTTGGGTATTGAATT+GGG + Chr8:49939740-49939759 None:intergenic 25.0%
AATCGCTTATATACGTTTCA+AGG + Chr8:49940028-49940047 None:intergenic 30.0%
ATCAAATCCATCCAATCAAA+CGG + Chr8:49940524-49940543 None:intergenic 30.0%
CAATTCAATACCCAAAAATC+TGG - Chr8:49939739-49939758 MsG0880044835.01.T01:CDS 30.0%
TATTGCTAAGAAAGCTGATT+GGG - Chr8:49940094-49940113 MsG0880044835.01.T01:CDS 30.0%
TCATTTGAGAAAGTTAATCC+CGG + Chr8:49940245-49940264 None:intergenic 30.0%
TTATTGCTTTACTTTGGATG+AGG - Chr8:49940625-49940644 MsG0880044835.01.T01:CDS 30.0%
! TGCTGATTATTGCTTTACTT+TGG - Chr8:49940619-49940638 MsG0880044835.01.T01:CDS 30.0%
! TGTACATTTCCCAGATTTTT+GGG + Chr8:49939752-49939771 None:intergenic 30.0%
! TTGTACATTTCCCAGATTTT+TGG + Chr8:49939753-49939772 None:intergenic 30.0%
!! ATATTGAGTTTGTTGCTGAT+AGG - Chr8:49940453-49940472 MsG0880044835.01.T01:CDS 30.0%
!!! ATTTTCTGGTTGAAAAAACG+CGG - Chr8:49940212-49940231 MsG0880044835.01.T01:CDS 30.0%
!!! CAGATTTTTGGGTATTGAAT+TGG + Chr8:49939741-49939760 None:intergenic 30.0%
AAAGCTTCTGTTATGAATGG+TGG - Chr8:49940596-49940615 MsG0880044835.01.T01:CDS 35.0%
AAGGAGAAGAGAATACATCT+CGG + Chr8:49940009-49940028 None:intergenic 35.0%
ATGTTCAGAATCCGTTTGAT+TGG - Chr8:49940510-49940529 MsG0880044835.01.T01:CDS 35.0%
CAGAAAGCTTCTGTTATGAA+TGG - Chr8:49940593-49940612 MsG0880044835.01.T01:CDS 35.0%
GTATTGCTAAGAAAGCTGAT+TGG - Chr8:49940093-49940112 MsG0880044835.01.T01:CDS 35.0%
TACTTCTTTCATGAATCTCC+CGG + Chr8:49939928-49939947 None:intergenic 35.0%
TCAGCTTTCTTAGCAATACA+CGG + Chr8:49940092-49940111 None:intergenic 35.0%
TGATGCAGTTGAGATTGAAA+GGG - Chr8:49940367-49940386 MsG0880044835.01.T01:CDS 35.0%
TTTGTTGCTGATAGGTTGTT+GGG - Chr8:49940461-49940480 MsG0880044835.01.T01:CDS 35.0%
! ATGGATTTGATTTCGCTTCA+GGG - Chr8:49940533-49940552 MsG0880044835.01.T01:CDS 35.0%
! GTTGAAGGAATTTTCTTCTC+CGG - Chr8:49940176-49940195 MsG0880044835.01.T01:CDS 35.0%
AAAAAACGCGGTTTAATGCC+GGG - Chr8:49940224-49940243 MsG0880044835.01.T01:CDS 40.0%
GAAAAAACGCGGTTTAATGC+CGG - Chr8:49940223-49940242 MsG0880044835.01.T01:CDS 40.0%
GTGATGCAGTTGAGATTGAA+AGG - Chr8:49940366-49940385 MsG0880044835.01.T01:CDS 40.0%
TCAGAATCCGTTTGATTGGA+TGG - Chr8:49940514-49940533 MsG0880044835.01.T01:CDS 40.0%
! AATTTCTTTGAGAAGCGCGT+TGG - Chr8:49940563-49940582 MsG0880044835.01.T01:CDS 40.0%
! ATTTCTTTGAGAAGCGCGTT+GGG - Chr8:49940564-49940583 MsG0880044835.01.T01:CDS 40.0%
! GATGGATTTGATTTCGCTTC+AGG - Chr8:49940532-49940551 MsG0880044835.01.T01:CDS 40.0%
! GGATGTTTTCAATAGCGATC+TGG + Chr8:49939983-49940002 None:intergenic 40.0%
! GTTGTTCCAGTGTTGAAGAT+CGG + Chr8:49939823-49939842 None:intergenic 40.0%
! GTTTGTTGCTGATAGGTTGT+TGG - Chr8:49940460-49940479 MsG0880044835.01.T01:CDS 40.0%
! TGGATTTGATTTCGCTTCAG+GGG - Chr8:49940534-49940553 MsG0880044835.01.T01:CDS 40.0%
!! AAGCTGATTGGGCTTTGAAA+TGG - Chr8:49940105-49940124 MsG0880044835.01.T01:CDS 40.0%
!!! GTTGCTTTTGCTTGTGTTGA+AGG - Chr8:49940161-49940180 MsG0880044835.01.T01:CDS 40.0%
AAAAAGCCGAAGCCTCTTTC+TGG - Chr8:49939772-49939791 MsG0880044835.01.T01:CDS 45.0%
GAAGAGAATACATCTCGGAG+TGG + Chr8:49940004-49940023 None:intergenic 45.0%
GAGCTTATTTCGTGTGACGA+AGG - Chr8:49940263-49940282 MsG0880044835.01.T01:CDS 45.0%
GATAGGTTGTTGGGTGAACT+TGG - Chr8:49940470-49940489 MsG0880044835.01.T01:CDS 45.0%
TTTCCTCCGATCTTCAACAC+TGG - Chr8:49939814-49939833 MsG0880044835.01.T01:CDS 45.0%
! GGAAAGATCGACTTCTTCAG+CGG + Chr8:49939799-49939818 None:intergenic 45.0%
! TTTCTTTGAGAAGCGCGTTG+GGG - Chr8:49940565-49940584 MsG0880044835.01.T01:CDS 45.0%
GAAAATCGCGCAGAAGCTTC+CGG + Chr8:49940198-49940217 None:intergenic 50.0%
TTCTTCAGCGGTCCAGAAAG+AGG + Chr8:49939787-49939806 None:intergenic 50.0%
! ACCATAGAAAGCACGCGCTT+CGG + Chr8:49939958-49939977 None:intergenic 50.0%
! GAAGCTTCTGCGCGATTTTC+TGG - Chr8:49940198-49940217 MsG0880044835.01.T01:CDS 50.0%
! GTTCCAGTGTTGAAGATCGG+AGG + Chr8:49939820-49939839 None:intergenic 50.0%
!! GTTTTCGAGGACGATACCGT+CGG + Chr8:49939901-49939920 None:intergenic 50.0%
AGCGGTCCAGAAAGAGGCTT+CGG + Chr8:49939781-49939800 None:intergenic 55.0%
ATCGTCCTCGAAAACCTCGC+CGG - Chr8:49939906-49939925 MsG0880044835.01.T01:CDS 55.0%
CACTGGAACAACCTCACCGA+CGG - Chr8:49939831-49939850 MsG0880044835.01.T01:CDS 55.0%
CGAGGATGTGAGAGACGAAG+TGG + Chr8:49939863-49939882 None:intergenic 55.0%
CTTTCATGAATCTCCCGGCG+AGG + Chr8:49939923-49939942 None:intergenic 55.0%
GAAGCTGAGTTCAGAGCGAG+TGG - Chr8:49940331-49940350 MsG0880044835.01.T01:CDS 55.0%
TCCGAAGCGCGTGCTTTCTA+TGG - Chr8:49939954-49939973 MsG0880044835.01.T01:CDS 55.0%
TTCATCCCAACCAACGCGCA+AGG + Chr8:49940413-49940432 None:intergenic 55.0%
!! CACTTCCTTGCGCGTTGGTT+GGG - Chr8:49940405-49940424 MsG0880044835.01.T01:CDS 55.0%
!! TGCGCGTTGGTTGGGATGAA+CGG - Chr8:49940413-49940432 MsG0880044835.01.T01:CDS 55.0%
GAGGACGATACCGTCGGAAG+CGG + Chr8:49939895-49939914 None:intergenic 60.0%
TCGTCCTCGAAAACCTCGCC+GGG - Chr8:49939907-49939926 MsG0880044835.01.T01:CDS 60.0%
! ATCTCCCGGCGAGGTTTTCG+AGG + Chr8:49939914-49939933 None:intergenic 60.0%
! GCACTTCCTTGCGCGTTGGT+TGG - Chr8:49940404-49940423 MsG0880044835.01.T01:CDS 60.0%
ACCGTCGGAAGCGGCGAAGA+AGG + Chr8:49939886-49939905 None:intergenic 65.0%
CGACGCACTTCCTTGCGCGT+TGG - Chr8:49940400-49940419 MsG0880044835.01.T01:CDS 65.0%
GACGAAGTGGCGTTCGCCGT+CGG + Chr8:49939850-49939869 None:intergenic 65.0%
GCCTTCTTCGCCGCTTCCGA+CGG - Chr8:49939882-49939901 MsG0880044835.01.T01:CDS 65.0%
AGTGGCGTTCGCCGTCGGTG+AGG + Chr8:49939845-49939864 None:intergenic 70.0%
CGGAAGCGGCGAAGAAGGCG+AGG + Chr8:49939881-49939900 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 49939732 49940655 49939732 ID=MsG0880044835.01;Name=MsG0880044835.01
Chr8 mRNA 49939732 49940655 49939732 ID=MsG0880044835.01.T01;Parent=MsG0880044835.01;Name=MsG0880044835.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|307
Chr8 exon 49939732 49940655 49939732 ID=MsG0880044835.01.T01:exon:20911;Parent=MsG0880044835.01.T01
Chr8 CDS 49939732 49940655 49939732 ID=MsG0880044835.01.T01:cds;Parent=MsG0880044835.01.T01
Gene Sequence

>MsG0880044835.01.T01

ATGTTCCCAATTCAATACCCAAAAATCTGGGAAATGTACAAAAAAGCCGAAGCCTCTTTCTGGACCGCTGAAGAAGTCGATCTTTCCTCCGATCTTCAACACTGGAACAACCTCACCGACGGCGAACGCCACTTCGTCTCTCACATCCTCGCCTTCTTCGCCGCTTCCGACGGTATCGTCCTCGAAAACCTCGCCGGGAGATTCATGAAAGAAGTACAAATCTCCGAAGCGCGTGCTTTCTATGGTTTCCAGATCGCTATTGAAAACATCCACTCCGAGATGTATTCTCTTCTCCTTGAAACGTATATAAGCGATTCTGCTGAAAAGAATCGATTATTTCACGCAATTGACACAATTCCGTGTATTGCTAAGAAAGCTGATTGGGCTTTGAAATGGATCGATTCAAGCGATTCTTTTGCAGAAAGAATCGTTGCTTTTGCTTGTGTTGAAGGAATTTTCTTCTCCGGAAGCTTCTGCGCGATTTTCTGGTTGAAAAAACGCGGTTTAATGCCGGGATTAACTTTCTCAAATGAGCTTATTTCGTGTGACGAAGGTTTACACTGTGATTTCGCTTGTTTGTTATACTCGCTATTGCGTCAGAAGCTGAGTTCAGAGCGAGTGGTGAAGATTGTTCGTGATGCAGTTGAGATTGAAAGGGAGTTTGTATGCGACGCACTTCCTTGCGCGTTGGTTGGGATGAACGGTGAATTGATGAGTCAGTATATTGAGTTTGTTGCTGATAGGTTGTTGGGTGAACTTGGTTGTGAGAAAGTTTATAATGTTCAGAATCCGTTTGATTGGATGGATTTGATTTCGCTTCAGGGGAAGACGAATTTCTTTGAGAAGCGCGTTGGGGAGTATCAGAAAGCTTCTGTTATGAATGGTGGTGCTGATTATTGCTTTACTTTGGATGAGGATTTTTAG

Protein sequence

>MsG0880044835.01.T01

MFPIQYPKIWEMYKKAEASFWTAEEVDLSSDLQHWNNLTDGERHFVSHILAFFAASDGIVLENLAGRFMKEVQISEARAFYGFQIAIENIHSEMYSLLLETYISDSAEKNRLFHAIDTIPCIAKKADWALKWIDSSDSFAERIVAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISCDEGLHCDFACLLYSLLRQKLSSERVVKIVRDAVEIEREFVCDALPCALVGMNGELMSQYIEFVADRLLGELGCEKVYNVQNPFDWMDLISLQGKTNFFEKRVGEYQKASVMNGGADYCFTLDEDF*