AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004006.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004006.01.T01 MTR_1g072690 88.767 365 34 2 1 362 1 361 0.0 590
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004006.01.T01 AT4G13670 52.316 367 157 9 3 362 1 356 6.12e-112 332
MsG0180004006.01.T01 AT4G13670 50.992 353 155 9 3 348 1 342 8.97e-102 305
MsG0180004006.01.T01 AT4G13670 53.731 268 114 6 3 265 1 263 1.08e-77 241
MsG0180004006.01.T01 AT4G13670 53.731 268 114 6 3 265 1 263 1.08e-77 241

Find 104 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACTTGAGGTTCAATATT+TGG 0.133095 1:+71702997 None:intergenic
TCTTCTTGAGAGGCTATATT+TGG 0.177538 1:-71704582 MsG0180004006.01.T01:CDS
ATAGGAAGCATTGCTAAAAT+TGG 0.187653 1:-71705302 MsG0180004006.01.T01:intron
GATGGGATGCTTCTGTTTCT+TGG 0.211385 1:+71703804 None:intergenic
ATGAAATGCCTTCAATGTTC+TGG 0.265689 1:-71703686 MsG0180004006.01.T01:CDS
AGAGGCTATATTTGGATATA+AGG 0.293358 1:-71704574 MsG0180004006.01.T01:CDS
GGTAAAGGAATTGAATTTAT+AGG 0.293857 1:+71705901 None:intergenic
GGTGTTGAAGGATAAGAATT+TGG 0.307976 1:-71705636 MsG0180004006.01.T01:CDS
ACCTTCGTCGAAAGTCTTGA+TGG 0.348199 1:+71703743 None:intergenic
TTTGAGAAATGAGAGGGTTT+TGG 0.351215 1:+71705937 None:intergenic
CGACGAAGGTTAGAGTTGAT+AGG 0.357851 1:-71703730 MsG0180004006.01.T01:CDS
CATTGTGAAACGGCGGGAAT+TGG 0.358756 1:+71706027 None:intergenic
GAGTTCTAGGAAGTTGAAAA+TGG 0.370611 1:-71703890 MsG0180004006.01.T01:intron
AGAGTTACAGTGACGGTTAT+GGG 0.371248 1:+71705983 None:intergenic
TAGAGTTACAGTGACGGTTA+TGG 0.378603 1:+71705982 None:intergenic
TTGAATTTGAAGTTTAAGTT+CGG 0.384376 1:+71705742 None:intergenic
AGATGAAGTGGAAGAAGAAA+AGG 0.404194 1:-71705561 MsG0180004006.01.T01:CDS
GAATGTTGCGGCGTTGCTAC+AGG 0.410320 1:-71705657 MsG0180004006.01.T01:CDS
GTTAGTGCTTGAGGAGGAAA+AGG 0.421005 1:-71705585 MsG0180004006.01.T01:CDS
GTGTTCTTCGCGATCGGGAA+TGG 0.425539 1:+71705868 None:intergenic
AGAAGAGGTTCAAAAGTTGC+AGG 0.426132 1:-71705447 MsG0180004006.01.T01:intron
CAAATATTGAACCTCAAGTA+AGG 0.426753 1:-71702996 MsG0180004006.01.T01:CDS
TAGGAAGCATTGCTAAAATT+GGG 0.430808 1:-71705301 MsG0180004006.01.T01:CDS
TGAAATGCCTTCAATGTTCT+GGG 0.435833 1:-71703685 MsG0180004006.01.T01:CDS
TATTGTTTAACAGTCTTCGT+TGG 0.443547 1:-71704636 MsG0180004006.01.T01:intron
CAGCGGTGTTCTTCGCGATC+GGG 0.449847 1:+71705863 None:intergenic
GACGAAGGTTAGAGTTGATA+GGG 0.456347 1:-71703729 MsG0180004006.01.T01:CDS
CCAGCGGTGTTCTTCGCGAT+CGG 0.462132 1:+71705862 None:intergenic
AATGGAGCAGTGAGCGGTAA+AGG 0.462535 1:+71705886 None:intergenic
GAAGCCATCGTTGTTCTTCA+CGG 0.463148 1:+71705837 None:intergenic
TACCTTGCCCAGAACATTGA+AGG 0.467337 1:+71703678 None:intergenic
ATTGTTTAACAGTCTTCGTT+GGG 0.467627 1:-71704635 MsG0180004006.01.T01:intron
TCCCCAAGGAGGAAAACTCT+TGG 0.467714 1:+71703782 None:intergenic
GACGTCATGATCCCATCCTC+AGG 0.480208 1:+71704608 None:intergenic
TCGTTGGGCATCCCTGAGGA+TGG 0.484266 1:-71704620 MsG0180004006.01.T01:CDS
CTGAGCGTGCTGTGAAGACT+TGG 0.485165 1:-71705228 MsG0180004006.01.T01:CDS
CAAGGTATCTTGTTGTGTAC+AGG 0.491562 1:-71703662 MsG0180004006.01.T01:intron
GAATCGATGGAATCGTGAAC+GGG 0.492158 1:-71705786 MsG0180004006.01.T01:CDS
GGCGTTGCTACAGGTGTTGA+AGG 0.493616 1:-71705648 MsG0180004006.01.T01:CDS
GGAGGAAAAGGAAGATGAAG+TGG 0.494506 1:-71705573 MsG0180004006.01.T01:CDS
GGAATCTGTTGAGGTTGTAA+AGG 0.496533 1:-71705540 MsG0180004006.01.T01:CDS
TCAGAGGAGGTTAGTGCTTG+AGG 0.509466 1:-71705594 MsG0180004006.01.T01:CDS
TTTGGTGGGGAAAGAGAGTG+TGG 0.512214 1:+71705955 None:intergenic
TGTTGAGAAACCTGCTGCTA+AGG 0.513290 1:-71705507 MsG0180004006.01.T01:CDS
AAGAATTTGGTTCTTGAGAG+TGG 0.516277 1:-71705623 MsG0180004006.01.T01:CDS
TCCTCCTTGGGGAAAATCGG+TGG 0.518446 1:-71703772 MsG0180004006.01.T01:CDS
TGAGGTTGTAAAGGAGATTG+TGG 0.520541 1:-71705531 MsG0180004006.01.T01:CDS
AGAAAAGAGGCCGTGGATTG+TGG 0.525209 1:+71706050 None:intergenic
CAAGGAGGAAAACTCTTGGA+TGG 0.534791 1:+71703786 None:intergenic
ATTGCAGAATGTGATGGATC+TGG 0.539845 1:-71703024 MsG0180004006.01.T01:intron
ATGAGGTTTGAGAAATGAGA+GGG 0.543349 1:+71705931 None:intergenic
CGATGCGGTTTCGAATGTTG+CGG 0.545301 1:-71705669 MsG0180004006.01.T01:CDS
TTGAGAGTGGTTCTAGTCAG+AGG 0.547189 1:-71705610 MsG0180004006.01.T01:CDS
CGTTGGGCATCCCTGAGGAT+GGG 0.552075 1:-71704619 MsG0180004006.01.T01:CDS
AAAGGAATTGAATTTATAGG+AGG 0.553750 1:+71705904 None:intergenic
TTCTTCTACGTCTTCCGATG+CGG 0.555273 1:-71705684 MsG0180004006.01.T01:CDS
TGCCTTCAATGTTCTGGGCA+AGG 0.563469 1:-71703680 MsG0180004006.01.T01:CDS
GCACAATTATCCCTTACTTG+AGG 0.564833 1:+71702985 None:intergenic
TACAGTGACGGTTATGGGAA+CGG 0.566716 1:+71705988 None:intergenic
ATATTTGGATATAAGGACTA+CGG 0.568308 1:-71704567 MsG0180004006.01.T01:CDS
GATGGCTTCGCGAGGAGCAA+CGG 0.569267 1:-71705823 MsG0180004006.01.T01:CDS
AGTTGATAGGGGCAAGAACA+AGG 0.571690 1:-71703717 MsG0180004006.01.T01:CDS
AACCTCTGAAATTTCTGTCA+CGG 0.573766 1:+71703853 None:intergenic
GAGAGACATTGTGAAACGGC+GGG 0.577677 1:+71706021 None:intergenic
TCTTTGATTGCAGAATGTGA+TGG 0.581458 1:-71703030 MsG0180004006.01.T01:intron
CCGATCGCGAAGAACACCGC+TGG 0.583146 1:-71705862 MsG0180004006.01.T01:CDS
CCTCCTTGGGGAAAATCGGT+GGG 0.583402 1:-71703771 MsG0180004006.01.T01:CDS
GGCTTCGCGAGGAGCAACGG+TGG 0.584221 1:-71705820 MsG0180004006.01.T01:CDS
CGCAACATTCGAAACCGCAT+CGG 0.587059 1:+71705670 None:intergenic
AGAATCGATGGAATCGTGAA+CGG 0.587227 1:-71705787 MsG0180004006.01.T01:CDS
CGGCGGGAATTGGAGAAAAG+AGG 0.588986 1:+71706037 None:intergenic
AAGGAGGAAAACTCTTGGAT+GGG 0.591075 1:+71703787 None:intergenic
AAGATATAATTCGATGCTCG+AGG 0.592000 1:+71705717 None:intergenic
TCGGAAGACGTAGAAGAAGA+AGG 0.592316 1:+71705689 None:intergenic
GGATTCGCGAGGAGAATCGA+TGG 0.592474 1:-71705799 MsG0180004006.01.T01:CDS
TTACTCGGGTGAGGAGGACA+TGG 0.594106 1:-71705275 MsG0180004006.01.T01:CDS
GAATTGGAGAAAAGAGGCCG+TGG 0.594938 1:+71706043 None:intergenic
AAATATTGAACCTCAAGTAA+GGG 0.601280 1:-71702995 MsG0180004006.01.T01:CDS
AATGAGGTTTGAGAAATGAG+AGG 0.606074 1:+71705930 None:intergenic
TAGAGAGAGACATTGTGAAA+CGG 0.607092 1:+71706017 None:intergenic
AACAGGTTCTGAAGGAGAAG+AGG 0.607825 1:-71705462 MsG0180004006.01.T01:CDS
GAATTCTCTAGCTTCTCAAG+TGG 0.612169 1:-71705253 MsG0180004006.01.T01:CDS
AGAAGAAAAGGAATCTGTTG+AGG 0.612843 1:-71705549 MsG0180004006.01.T01:CDS
ATGGAAATATCCACAATCCA+CGG 0.621840 1:-71706060 None:intergenic
AATTCTCTAGCTTCTCAAGT+GGG 0.622250 1:-71705252 MsG0180004006.01.T01:CDS
TTCCGTGACAGAAATTTCAG+AGG 0.631894 1:-71703855 MsG0180004006.01.T01:CDS
AGAGTGGTTCTAGTCAGAGG+AGG 0.634878 1:-71705607 MsG0180004006.01.T01:CDS
GTGACGGTTATGGGAACGGT+AGG 0.637180 1:+71705992 None:intergenic
CTGCTGCTAAGGTTGAAAAG+AGG 0.637914 1:-71705496 MsG0180004006.01.T01:CDS
GTTGTTCTTCACGGAGCCAG+CGG 0.640610 1:+71705846 None:intergenic
ATCGGGAATGGAGCAGTGAG+CGG 0.642837 1:+71705880 None:intergenic
AGATATAATTCGATGCTCGA+GGG 0.643521 1:+71705718 None:intergenic
ACCATCAAGACTTTCGACGA+AGG 0.643727 1:-71703744 MsG0180004006.01.T01:CDS
GGAGCAACGGTGGATTCGCG+AGG 0.645147 1:-71705810 MsG0180004006.01.T01:CDS
AGAGAGACATTGTGAAACGG+CGG 0.649579 1:+71706020 None:intergenic
TGACGTCTAATCTTCTTGAG+AGG 0.653400 1:-71704592 MsG0180004006.01.T01:CDS
ACGTCATGATCCCATCCTCA+GGG 0.653901 1:+71704609 None:intergenic
ACGAAGGTTAGAGTTGATAG+GGG 0.654349 1:-71703728 MsG0180004006.01.T01:CDS
GAGGAGGTTAGTGCTTGAGG+AGG 0.654807 1:-71705591 MsG0180004006.01.T01:CDS
GATAGGGGCAAGAACAAGGA+CGG 0.659961 1:-71703713 MsG0180004006.01.T01:CDS
AGAACAACGATGGCTTCGCG+AGG 0.677962 1:-71705831 MsG0180004006.01.T01:CDS
GGCTCCGTGAAGAACAACGA+TGG 0.678007 1:-71705841 MsG0180004006.01.T01:CDS
GGAGTTTAGAGTTACAGTGA+CGG 0.682062 1:+71705976 None:intergenic
GTCTTCGTTGGGCATCCCTG+AGG 0.685757 1:-71704624 MsG0180004006.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCATACATAATATATTT+AGG + Chr1:71705922-71705941 None:intergenic 10.0%
!! AAATTATCCTTATTAACTAT+TGG + Chr1:71704218-71704237 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG - Chr1:71704217-71704236 MsG0180004006.01.T01:intron 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG + Chr1:71704091-71704110 None:intergenic 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG + Chr1:71704194-71704213 None:intergenic 15.0%
!!! TTTTTTTTTTTCATATGTTG+AGG + Chr1:71704913-71704932 None:intergenic 15.0%
!! AAACTAACCAATAGTTAATA+AGG - Chr1:71704208-71704227 MsG0180004006.01.T01:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + Chr1:71704131-71704150 None:intergenic 20.0%
!! ATTATGTATGATATCAACTT+TGG - Chr1:71705928-71705947 MsG0180004006.01.T01:CDS 20.0%
!! GATAAAGAATCAAATACAAA+TGG + Chr1:71705106-71705125 None:intergenic 20.0%
!! TTATATAACAAACATACCTT+CGG + Chr1:71704524-71704543 None:intergenic 20.0%
!!! TAAAACATTAGCAGATTTTT+TGG + Chr1:71704501-71704520 None:intergenic 20.0%
!!! TTGAATTTGAAGTTTAAGTT+CGG + Chr1:71703282-71703301 None:intergenic 20.0%
! AAAGGAATTGAATTTATAGG+AGG + Chr1:71703120-71703139 None:intergenic 25.0%
! AAATATTGAACCTCAAGTAA+GGG - Chr1:71706026-71706045 MsG0180004006.01.T01:exon 25.0%
! AACTAAAAGTGAAAACAACA+GGG + Chr1:71705396-71705415 None:intergenic 25.0%
! AATTTATAGGAGGAAAAATG+AGG + Chr1:71703110-71703129 None:intergenic 25.0%
! ATATTTGGATATAAGGACTA+CGG - Chr1:71704454-71704473 MsG0180004006.01.T01:intron 25.0%
! CATAAATAATGACCATTACA+TGG - Chr1:71705866-71705885 MsG0180004006.01.T01:CDS 25.0%
! CTTTACATTGAATACATGTT+TGG - Chr1:71704660-71704679 MsG0180004006.01.T01:intron 25.0%
! GAAGAGAATAATAACTGTAT+TGG - Chr1:71703856-71703875 MsG0180004006.01.T01:CDS 25.0%
! GGTAAAGGAATTGAATTTAT+AGG + Chr1:71703123-71703142 None:intergenic 25.0%
! TAAGAGTAACCACAAAAATA+TGG + Chr1:71704584-71704603 None:intergenic 25.0%
! TCATTATTTGACAGTTATCA+AGG - Chr1:71704990-71705009 MsG0180004006.01.T01:intron 25.0%
!! ACCTAAAAGCTTAAACTTTT+AGG + Chr1:71705025-71705044 None:intergenic 25.0%
!! TCCTAAAAGTTTAAGCTTTT+AGG - Chr1:71705021-71705040 MsG0180004006.01.T01:intron 25.0%
!! TTTAACTGTCATGATTTTGA+TGG - Chr1:71704354-71704373 MsG0180004006.01.T01:intron 25.0%
!!! AGTATATGCTATGCTATTTT+TGG - Chr1:71704327-71704346 MsG0180004006.01.T01:intron 25.0%
!!! TTAACTGTCATGATTTTGAT+GGG - Chr1:71704355-71704374 MsG0180004006.01.T01:intron 25.0%
AACAAAACAACCTAGAGAAA+AGG + Chr1:71705483-71705502 None:intergenic 30.0%
AATGGCTATTGTGTGAATTT+GGG - Chr1:71705743-71705762 MsG0180004006.01.T01:CDS 30.0%
ACATTCTGCAATCAAAGAAA+CGG + Chr1:71705989-71706008 None:intergenic 30.0%
AGAGGCTATATTTGGATATA+AGG - Chr1:71704447-71704466 MsG0180004006.01.T01:intron 30.0%
AGTTCAGTTTGATGTGATAA+TGG - Chr1:71705725-71705744 MsG0180004006.01.T01:CDS 30.0%
ATAGTAGTATTCATAACAGC+AGG - Chr1:71705779-71705798 MsG0180004006.01.T01:CDS 30.0%
ATTAGAGCTTCACAAAATGA+CGG + Chr1:71703941-71703960 None:intergenic 30.0%
ATTGTTTAACAGTCTTCGTT+GGG - Chr1:71704386-71704405 MsG0180004006.01.T01:intron 30.0%
CAAATATTGAACCTCAAGTA+AGG - Chr1:71706025-71706044 MsG0180004006.01.T01:exon 30.0%
CAACTAAAAGTGAAAACAAC+AGG + Chr1:71705397-71705416 None:intergenic 30.0%
CTTACTTGAGGTTCAATATT+TGG + Chr1:71706027-71706046 None:intergenic 30.0%
GAAAATCAACTGTGTGATAA+TGG - Chr1:71705698-71705717 MsG0180004006.01.T01:CDS 30.0%
GTGATGAATTGTGTTACAAT+AGG - Chr1:71703701-71703720 MsG0180004006.01.T01:CDS 30.0%
TAATGGCTATTGTGTGAATT+TGG - Chr1:71705742-71705761 MsG0180004006.01.T01:CDS 30.0%
TATTGTTTAACAGTCTTCGT+TGG - Chr1:71704385-71704404 MsG0180004006.01.T01:intron 30.0%
TCAAATAACTCGGATGAATT+GGG + Chr1:71705597-71705616 None:intergenic 30.0%
TCTTTATCAATCTGAGTTCT+AGG - Chr1:71705118-71705137 MsG0180004006.01.T01:intron 30.0%
TGTTATTAGTATCTACACGT+TGG - Chr1:71704763-71704782 MsG0180004006.01.T01:intron 30.0%
TTCAAATAACTCGGATGAAT+TGG + Chr1:71705598-71705617 None:intergenic 30.0%
TTCATATCTTGTCTCTTCAT+TGG + Chr1:71705083-71705102 None:intergenic 30.0%
TTTATGAAGACCTAAATGTG+AGG + Chr1:71705852-71705871 None:intergenic 30.0%
! AAGAATTGATTTCCTTACTG+GGG + Chr1:71705430-71705449 None:intergenic 30.0%
! CAAGAATTGATTTCCTTACT+GGG + Chr1:71705431-71705450 None:intergenic 30.0%
! CTAAAATTGGGATTTTACTC+GGG - Chr1:71703732-71703751 MsG0180004006.01.T01:CDS 30.0%
! GCTAAAATTGGGATTTTACT+CGG - Chr1:71703731-71703750 MsG0180004006.01.T01:CDS 30.0%
! TCAAGAATTGATTTCCTTAC+TGG + Chr1:71705432-71705451 None:intergenic 30.0%
! TGTAGCTTTTCTCAAAATCA+CGG - Chr1:71703962-71703981 MsG0180004006.01.T01:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG - Chr1:71704300-71704319 MsG0180004006.01.T01:intron 30.0%
!! AACATGCAACCATATTTTTG+TGG - Chr1:71704572-71704591 MsG0180004006.01.T01:CDS 30.0%
!! ATAGGAAGCATTGCTAAAAT+TGG - Chr1:71703719-71703738 MsG0180004006.01.T01:CDS 30.0%
!! ATTTGGATTGATGATGACAT+TGG + Chr1:71704006-71704025 None:intergenic 30.0%
!! TAGGAAGCATTGCTAAAATT+GGG - Chr1:71703720-71703739 MsG0180004006.01.T01:CDS 30.0%
!! TATTTTTGTTACTGCGTGTT+TGG - Chr1:71703882-71703901 MsG0180004006.01.T01:CDS 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + Chr1:71704299-71704318 None:intergenic 30.0%
AAGATATAATTCGATGCTCG+AGG + Chr1:71703307-71703326 None:intergenic 35.0%
AATGAGGTTTGAGAAATGAG+AGG + Chr1:71703094-71703113 None:intergenic 35.0%
ACAATGTCATCTTGTTGACA+AGG - Chr1:71705560-71705579 MsG0180004006.01.T01:CDS 35.0%
ACACTATAATAGCACCAAGT+TGG + Chr1:71704030-71704049 None:intergenic 35.0%
AGAAGAAAAGGAATCTGTTG+AGG - Chr1:71703472-71703491 MsG0180004006.01.T01:intron 35.0%
AGATATAATTCGATGCTCGA+GGG + Chr1:71703306-71703325 None:intergenic 35.0%
AGATGAAGTGGAAGAAGAAA+AGG - Chr1:71703460-71703479 MsG0180004006.01.T01:intron 35.0%
ATGAAATGCCTTCAATGTTC+TGG - Chr1:71705335-71705354 MsG0180004006.01.T01:intron 35.0%
ATGAGGTTTGAGAAATGAGA+GGG + Chr1:71703093-71703112 None:intergenic 35.0%
CATCAATCCAAATTCCAACT+TGG - Chr1:71704013-71704032 MsG0180004006.01.T01:intron 35.0%
GACTTGAGACTTGAGATATA+TGG + Chr1:71705961-71705980 None:intergenic 35.0%
GCTCAGCATCTTTATTATAC+TGG - Chr1:71705893-71705912 MsG0180004006.01.T01:CDS 35.0%
GTTAGTCAATCCACTCAAAT+TGG - Chr1:71705644-71705663 MsG0180004006.01.T01:CDS 35.0%
TAGAGAGAGACATTGTGAAA+CGG + Chr1:71703007-71703026 None:intergenic 35.0%
TCTAGTTAAGCCAATTTGAG+TGG + Chr1:71705657-71705676 None:intergenic 35.0%
TCTTCTTGAGAGGCTATATT+TGG - Chr1:71704439-71704458 MsG0180004006.01.T01:intron 35.0%
TCTTGATGGAAATCTGAACA+AGG - Chr1:71704867-71704886 MsG0180004006.01.T01:intron 35.0%
TCTTTGATTGCAGAATGTGA+TGG - Chr1:71705991-71706010 MsG0180004006.01.T01:CDS 35.0%
TGAAATGCCTTCAATGTTCT+GGG - Chr1:71705336-71705355 MsG0180004006.01.T01:intron 35.0%
TGAATATATAAGCCCCAGTA+AGG - Chr1:71705415-71705434 MsG0180004006.01.T01:intron 35.0%
TGATTTCAGCAAATTCATGG+TGG - Chr1:71703913-71703932 MsG0180004006.01.T01:intron 35.0%
! AATAGCACCAAGTTGGAATT+TGG + Chr1:71704023-71704042 None:intergenic 35.0%
! AATTCTCTAGCTTCTCAAGT+GGG - Chr1:71703769-71703788 MsG0180004006.01.T01:CDS 35.0%
! ATCTGCTAATGTTTTACCGA+AGG - Chr1:71704505-71704524 MsG0180004006.01.T01:intron 35.0%
! CTCGAGGTATTCAAATAACT+CGG + Chr1:71705607-71705626 None:intergenic 35.0%
! GGTGTTGAAGGATAAGAATT+TGG - Chr1:71703385-71703404 MsG0180004006.01.T01:intron 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG + Chr1:71704169-71704188 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG + Chr1:71704165-71704184 None:intergenic 35.0%
! TTTGAGAAATGAGAGGGTTT+TGG + Chr1:71703087-71703106 None:intergenic 35.0%
!! AACCTCTGAAATTTCTGTCA+CGG + Chr1:71705171-71705190 None:intergenic 35.0%
!! AAGAATTTGGTTCTTGAGAG+TGG - Chr1:71703398-71703417 MsG0180004006.01.T01:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + Chr1:71704065-71704084 None:intergenic 35.0%
!! AGCTTAAACTTTTAGGAGAG+TGG + Chr1:71705018-71705037 None:intergenic 35.0%
!! GAGTTCTAGGAAGTTGAAAA+TGG - Chr1:71705131-71705150 MsG0180004006.01.T01:intron 35.0%
!! GCTTAAACTTTTAGGAGAGT+GGG + Chr1:71705017-71705036 None:intergenic 35.0%
!! GTACTTTTTGCTCTTGAGTA+CGG - Chr1:71704547-71704566 MsG0180004006.01.T01:CDS 35.0%
!! TGAGAGTATACCTTTTCTCT+AGG - Chr1:71705470-71705489 MsG0180004006.01.T01:CDS 35.0%
!!! TTCACATGGTTTTTTTCTCG+AGG + Chr1:71705623-71705642 None:intergenic 35.0%
AACTCGGATGAATTGGGATT+AGG + Chr1:71705591-71705610 None:intergenic 40.0%
AAGGAGGAAAACTCTTGGAT+GGG + Chr1:71705237-71705256 None:intergenic 40.0%
AGAAGAGGTTCAAAAGTTGC+AGG - Chr1:71703574-71703593 MsG0180004006.01.T01:intron 40.0%
AGAATCGATGGAATCGTGAA+CGG - Chr1:71703234-71703253 MsG0180004006.01.T01:intron 40.0%
AGAGTTACAGTGACGGTTAT+GGG + Chr1:71703041-71703060 None:intergenic 40.0%
AGTATTCATAACAGCAGGCA+TGG - Chr1:71705784-71705803 MsG0180004006.01.T01:CDS 40.0%
ATGAAACACTGACAGAGACA+TGG + Chr1:71704804-71704823 None:intergenic 40.0%
CGGTGATTTCAGCAAATTCA+TGG - Chr1:71703910-71703929 MsG0180004006.01.T01:intron 40.0%
CTACAAAATCTACTACTCCC+AGG + Chr1:71705053-71705072 None:intergenic 40.0%
GATAGATGCCATGTCTCTAA+GGG - Chr1:71704828-71704847 MsG0180004006.01.T01:intron 40.0%
GCACAATTATCCCTTACTTG+AGG + Chr1:71706039-71706058 None:intergenic 40.0%
GGAATCTGTTGAGGTTGTAA+AGG - Chr1:71703481-71703500 MsG0180004006.01.T01:intron 40.0%
GGTGGTTTATCCTCACATTT+AGG - Chr1:71705839-71705858 MsG0180004006.01.T01:CDS 40.0%
GTCAACAAGATGACATTGTG+AGG + Chr1:71705559-71705578 None:intergenic 40.0%
TAGAGTTACAGTGACGGTTA+TGG + Chr1:71703042-71703061 None:intergenic 40.0%
TGACGTCTAATCTTCTTGAG+AGG - Chr1:71704429-71704448 MsG0180004006.01.T01:intron 40.0%
TGAGGTTGTAAAGGAGATTG+TGG - Chr1:71703490-71703509 MsG0180004006.01.T01:intron 40.0%
TGATAGATGCCATGTCTCTA+AGG - Chr1:71704827-71704846 MsG0180004006.01.T01:intron 40.0%
TGATGACATTGGCAGAATCA+CGG + Chr1:71703995-71704014 None:intergenic 40.0%
TGGATTGACTAACGTTCACA+TGG + Chr1:71705637-71705656 None:intergenic 40.0%
TTCCGTGACAGAAATTTCAG+AGG - Chr1:71705166-71705185 MsG0180004006.01.T01:intron 40.0%
TTGTTACTGCGTGTTTGGAT+TGG - Chr1:71703887-71703906 MsG0180004006.01.T01:intron 40.0%
! AGTGTTGTTGCTACATAGCA+TGG + Chr1:71704642-71704661 None:intergenic 40.0%
! ATCCAAGAGTTTTCCTCCTT+GGG - Chr1:71705237-71705256 MsG0180004006.01.T01:CDS 40.0%
! CAAGGTATCTTGTTGTGTAC+AGG - Chr1:71705359-71705378 MsG0180004006.01.T01:intron 40.0%
! GAATTCTCTAGCTTCTCAAG+TGG - Chr1:71703768-71703787 MsG0180004006.01.T01:CDS 40.0%
! GGAGTTTAGAGTTACAGTGA+CGG + Chr1:71703048-71703067 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG + Chr1:71704166-71704185 None:intergenic 40.0%
! GTGTTGTTGCTACATAGCAT+GGG + Chr1:71704641-71704660 None:intergenic 40.0%
! TTTTCCTCCTTGGGGAAAAT+CGG - Chr1:71705246-71705265 MsG0180004006.01.T01:CDS 40.0%
!! ACGAAGGTTAGAGTTGATAG+GGG - Chr1:71705293-71705312 MsG0180004006.01.T01:CDS 40.0%
!! AGAAATGAGAGGGTTTTGGT+GGG + Chr1:71703083-71703102 None:intergenic 40.0%
!! ATTGCAGAATGTGATGGATC+TGG - Chr1:71705997-71706016 MsG0180004006.01.T01:CDS 40.0%
!! CTAAGGGATTGGTCAATGAT+TGG - Chr1:71704844-71704863 MsG0180004006.01.T01:intron 40.0%
!! GACGAAGGTTAGAGTTGATA+GGG - Chr1:71705292-71705311 MsG0180004006.01.T01:CDS 40.0%
!! TGGTCAATGATTGGTCTTGA+TGG - Chr1:71704853-71704872 MsG0180004006.01.T01:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - Chr1:71704304-71704323 MsG0180004006.01.T01:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + Chr1:71704323-71704304 None:intergenic 40.0%
ACCATCAAGACTTTCGACGA+AGG - Chr1:71705277-71705296 MsG0180004006.01.T01:CDS 45.0%
ACCTTCGTCGAAAGTCTTGA+TGG + Chr1:71705281-71705300 None:intergenic 45.0%
AGAGAGACATTGTGAAACGG+CGG + Chr1:71703004-71703023 None:intergenic 45.0%
AGAGGTTCAGCAAAAAGTCG+TGG - Chr1:71705184-71705203 MsG0180004006.01.T01:intron 45.0%
AGAGTATGAGAAGCTCCAGT+TGG + Chr1:71705509-71705528 None:intergenic 45.0%
CAAGGAGGAAAACTCTTGGA+TGG + Chr1:71705238-71705257 None:intergenic 45.0%
CTGCTGCTAAGGTTGAAAAG+AGG - Chr1:71703525-71703544 MsG0180004006.01.T01:intron 45.0%
GAAGCCATCGTTGTTCTTCA+CGG + Chr1:71703187-71703206 None:intergenic 45.0%
GAATCGATGGAATCGTGAAC+GGG - Chr1:71703235-71703254 MsG0180004006.01.T01:intron 45.0%
GAGGTTCAGCAAAAAGTCGT+GGG - Chr1:71705185-71705204 MsG0180004006.01.T01:intron 45.0%
GCAGCTTCATTGGAGTCATT+GGG + Chr1:71705810-71705829 None:intergenic 45.0%
GGAGGAAAAGGAAGATGAAG+TGG - Chr1:71703448-71703467 MsG0180004006.01.T01:intron 45.0%
GGATGAATTGGGATTAGGAG+AGG + Chr1:71705586-71705605 None:intergenic 45.0%
GTTAGTGCTTGAGGAGGAAA+AGG - Chr1:71703436-71703455 MsG0180004006.01.T01:intron 45.0%
TACAGTGACGGTTATGGGAA+CGG + Chr1:71703036-71703055 None:intergenic 45.0%
TACCTTGCCCAGAACATTGA+AGG + Chr1:71705346-71705365 None:intergenic 45.0%
TCGGAAGACGTAGAAGAAGA+AGG + Chr1:71703335-71703354 None:intergenic 45.0%
TCTACACGTTGGTATCGTGT+CGG - Chr1:71704774-71704793 MsG0180004006.01.T01:intron 45.0%
TGACCAATCCCTTAGAGACA+TGG + Chr1:71704839-71704858 None:intergenic 45.0%
TGCTGAGCTCTTCCATGTAA+TGG + Chr1:71705881-71705900 None:intergenic 45.0%
TGTTGAGAAACCTGCTGCTA+AGG - Chr1:71703514-71703533 MsG0180004006.01.T01:intron 45.0%
TTCAATGTGACCACATGTGC+TGG + Chr1:71704723-71704742 None:intergenic 45.0%
TTCTTCTACGTCTTCCGATG+CGG - Chr1:71703337-71703356 MsG0180004006.01.T01:intron 45.0%
! AAGAGGACTGTTTTGCGAAC+AGG - Chr1:71703542-71703561 MsG0180004006.01.T01:intron 45.0%
! ATGCCATGTCTCTAAGGGAT+TGG - Chr1:71704833-71704852 MsG0180004006.01.T01:intron 45.0%
! ATTGGGATTTTACTCGGGTG+AGG - Chr1:71703737-71703756 MsG0180004006.01.T01:CDS 45.0%
! CATCCAAGAGTTTTCCTCCT+TGG - Chr1:71705236-71705255 MsG0180004006.01.T01:CDS 45.0%
! CGACGAAGGTTAGAGTTGAT+AGG - Chr1:71705291-71705310 MsG0180004006.01.T01:CDS 45.0%
! CTCTTTTCAACCTTAGCAGC+AGG + Chr1:71703527-71703546 None:intergenic 45.0%
! GATGGGATGCTTCTGTTTCT+TGG + Chr1:71705220-71705239 None:intergenic 45.0%
! TCCAAGAGTTTTCCTCCTTG+GGG - Chr1:71705238-71705257 MsG0180004006.01.T01:CDS 45.0%
! TTACTGCGTGTTTGGATTGG+CGG - Chr1:71703890-71703909 MsG0180004006.01.T01:intron 45.0%
!! AACAGGTTCTGAAGGAGAAG+AGG - Chr1:71703559-71703578 MsG0180004006.01.T01:intron 45.0%
!! AAGCTTTTAGGTGCAAGCCT+GGG - Chr1:71705033-71705052 MsG0180004006.01.T01:intron 45.0%
!! AGTTGATAGGGGCAAGAACA+AGG - Chr1:71705304-71705323 MsG0180004006.01.T01:intron 45.0%
!! GAAATGAGAGGGTTTTGGTG+GGG + Chr1:71703082-71703101 None:intergenic 45.0%
!! GAGAAATGAGAGGGTTTTGG+TGG + Chr1:71703084-71703103 None:intergenic 45.0%
!! GGTTGTTTTGTTCCTCCAAC+TGG - Chr1:71705491-71705510 MsG0180004006.01.T01:CDS 45.0%
!! GTTTTGCGAACAGGTTCTGA+AGG - Chr1:71703551-71703570 MsG0180004006.01.T01:intron 45.0%
!! TAAGCTTTTAGGTGCAAGCC+TGG - Chr1:71705032-71705051 MsG0180004006.01.T01:intron 45.0%
!! TTGAGAGTGGTTCTAGTCAG+AGG - Chr1:71703411-71703430 MsG0180004006.01.T01:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - Chr1:71704303-71704322 MsG0180004006.01.T01:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + Chr1:71704322-71704303 None:intergenic 45.0%
AATGGAGCAGTGAGCGGTAA+AGG + Chr1:71703138-71703157 None:intergenic 50.0%
ACGTCATGATCCCATCCTCA+GGG + Chr1:71704415-71704434 None:intergenic 50.0%
CAATGAAGCTGCGAGAGTTG+AGG - Chr1:71705818-71705837 MsG0180004006.01.T01:CDS 50.0%
CATTGTGAAACGGCGGGAAT+TGG + Chr1:71702997-71703016 None:intergenic 50.0%
CGATGCGGTTTCGAATGTTG+CGG - Chr1:71703352-71703371 MsG0180004006.01.T01:intron 50.0%
CGCAACATTCGAAACCGCAT+CGG + Chr1:71703354-71703373 None:intergenic 50.0%
CGCAGCTTCATTGGAGTCAT+TGG + Chr1:71705811-71705830 None:intergenic 50.0%
CTACACAACACCAGCACATG+TGG - Chr1:71704710-71704729 MsG0180004006.01.T01:intron 50.0%
CTACACGTTGGTATCGTGTC+GGG - Chr1:71704775-71704794 MsG0180004006.01.T01:intron 50.0%
CTCAACTCTCGCAGCTTCAT+TGG + Chr1:71705820-71705839 None:intergenic 50.0%
GAATTGGAGAAAAGAGGCCG+TGG + Chr1:71702981-71703000 None:intergenic 50.0%
GAGAGACATTGTGAAACGGC+GGG + Chr1:71703003-71703022 None:intergenic 50.0%
GATAGGGGCAAGAACAAGGA+CGG - Chr1:71705308-71705327 MsG0180004006.01.T01:intron 50.0%
GTATGAGAAGCTCCAGTTGG+AGG + Chr1:71705506-71705525 None:intergenic 50.0%
TCAGAGGAGGTTAGTGCTTG+AGG - Chr1:71703427-71703446 MsG0180004006.01.T01:intron 50.0%
TCCCCAAGGAGGAAAACTCT+TGG + Chr1:71705242-71705261 None:intergenic 50.0%
TTTGGTGGGGAAAGAGAGTG+TGG + Chr1:71703069-71703088 None:intergenic 50.0%
! AGAAAAGAGGCCGTGGATTG+TGG + Chr1:71702974-71702993 None:intergenic 50.0%
! TGCCTTCAATGTTCTGGGCA+AGG - Chr1:71705341-71705360 MsG0180004006.01.T01:intron 50.0%
!! AGAGTGGTTCTAGTCAGAGG+AGG - Chr1:71703414-71703433 MsG0180004006.01.T01:intron 50.0%
!! CACATGTGCTGGTGTTGTGT+AGG + Chr1:71704712-71704731 None:intergenic 50.0%
!! TGCTGGTGTTGTGTAGGTGA+TGG + Chr1:71704706-71704725 None:intergenic 50.0%
AGAACAACGATGGCTTCGCG+AGG - Chr1:71703190-71703209 MsG0180004006.01.T01:intron 55.0%
ATCGGGAATGGAGCAGTGAG+CGG + Chr1:71703144-71703163 None:intergenic 55.0%
CCTCCTTGGGGAAAATCGGT+GGG - Chr1:71705250-71705269 MsG0180004006.01.T01:CDS 55.0%
CGGCGGGAATTGGAGAAAAG+AGG + Chr1:71702987-71703006 None:intergenic 55.0%
CTGAGCGTGCTGTGAAGACT+TGG - Chr1:71703793-71703812 MsG0180004006.01.T01:CDS 55.0%
GAATGTTGCGGCGTTGCTAC+AGG - Chr1:71703364-71703383 MsG0180004006.01.T01:intron 55.0%
GACGTCATGATCCCATCCTC+AGG + Chr1:71704416-71704435 None:intergenic 55.0%
GAGGAGGTTAGTGCTTGAGG+AGG - Chr1:71703430-71703449 MsG0180004006.01.T01:intron 55.0%
GGATTCGCGAGGAGAATCGA+TGG - Chr1:71703222-71703241 MsG0180004006.01.T01:intron 55.0%
GGCTCCGTGAAGAACAACGA+TGG - Chr1:71703180-71703199 MsG0180004006.01.T01:intron 55.0%
GTGACGGTTATGGGAACGGT+AGG + Chr1:71703032-71703051 None:intergenic 55.0%
GTGTTCTTCGCGATCGGGAA+TGG + Chr1:71703156-71703175 None:intergenic 55.0%
GTTGTTCTTCACGGAGCCAG+CGG + Chr1:71703178-71703197 None:intergenic 55.0%
TCCTCCTTGGGGAAAATCGG+TGG - Chr1:71705249-71705268 MsG0180004006.01.T01:CDS 55.0%
TGAAGCTGCGAGAGTTGAGG+TGG - Chr1:71705821-71705840 MsG0180004006.01.T01:CDS 55.0%
TTACTCGGGTGAGGAGGACA+TGG - Chr1:71703746-71703765 MsG0180004006.01.T01:CDS 55.0%
! GGGATTTTACTCGGGTGAGG+AGG - Chr1:71703740-71703759 MsG0180004006.01.T01:CDS 55.0%
!! GGCGTTGCTACAGGTGTTGA+AGG - Chr1:71703373-71703392 MsG0180004006.01.T01:intron 55.0%
!!! CTTCCCACCGATTTTCCCCA+AGG + Chr1:71705256-71705275 None:intergenic 55.0%
CAGCGGTGTTCTTCGCGATC+GGG + Chr1:71703161-71703180 None:intergenic 60.0%
CCAGCGGTGTTCTTCGCGAT+CGG + Chr1:71703162-71703181 None:intergenic 60.0%
CGTTGGGCATCCCTGAGGAT+GGG - Chr1:71704402-71704421 MsG0180004006.01.T01:intron 60.0%
GATGGCTTCGCGAGGAGCAA+CGG - Chr1:71703198-71703217 MsG0180004006.01.T01:intron 60.0%
GTCTTCGTTGGGCATCCCTG+AGG - Chr1:71704397-71704416 MsG0180004006.01.T01:intron 60.0%
TCGTTGGGCATCCCTGAGGA+TGG - Chr1:71704401-71704420 MsG0180004006.01.T01:intron 60.0%
!!! CCCACCGATTTTCCCCAAGG+AGG + Chr1:71705253-71705272 None:intergenic 60.0%
CCGATCGCGAAGAACACCGC+TGG - Chr1:71703159-71703178 MsG0180004006.01.T01:intron 65.0%
! GGAGCAACGGTGGATTCGCG+AGG - Chr1:71703211-71703230 MsG0180004006.01.T01:intron 65.0%
GGCTTCGCGAGGAGCAACGG+TGG - Chr1:71703201-71703220 MsG0180004006.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 71702969 71706074 71702969 ID=MsG0180004006.01;Name=MsG0180004006.01
Chr1 mRNA 71702969 71706074 71702969 ID=MsG0180004006.01.T01;Parent=MsG0180004006.01;Name=MsG0180004006.01.T01;_AED=0.35;_eAED=0.37;_QI=45|1|0.8|1|1|1|5|0|372
Chr1 exon 71702969 71703039 71702969 ID=MsG0180004006.01.T01:exon:4929;Parent=MsG0180004006.01.T01
Chr1 exon 71703663 71703903 71703663 ID=MsG0180004006.01.T01:exon:4928;Parent=MsG0180004006.01.T01
Chr1 exon 71704517 71704645 71704517 ID=MsG0180004006.01.T01:exon:4927;Parent=MsG0180004006.01.T01
Chr1 exon 71705225 71705320 71705225 ID=MsG0180004006.01.T01:exon:4926;Parent=MsG0180004006.01.T01
Chr1 exon 71705448 71706074 71705448 ID=MsG0180004006.01.T01:exon:4925;Parent=MsG0180004006.01.T01
Chr1 five_prime_UTR 71706030 71706074 71706030 ID=MsG0180004006.01.T01:five_prime_utr;Parent=MsG0180004006.01.T01
Chr1 CDS 71705448 71706029 71705448 ID=MsG0180004006.01.T01:cds;Parent=MsG0180004006.01.T01
Chr1 CDS 71705225 71705320 71705225 ID=MsG0180004006.01.T01:cds;Parent=MsG0180004006.01.T01
Chr1 CDS 71704517 71704645 71704517 ID=MsG0180004006.01.T01:cds;Parent=MsG0180004006.01.T01
Chr1 CDS 71703663 71703903 71703663 ID=MsG0180004006.01.T01:cds;Parent=MsG0180004006.01.T01
Chr1 CDS 71702969 71703039 71702969 ID=MsG0180004006.01.T01:cds;Parent=MsG0180004006.01.T01
Gene Sequence

>MsG0180004006.01.T01

ATGTCTCTCTCTACACCTACCGTTCCCATAACCGTCACTGTAACTCTAAACTCCACACTCTCTTTCCCCACCAAAACCCTCTCATTTCTCAAACCTCATTTTTCCTCCTATAAATTCAATTCCTTTACCGCTCACTGCTCCATTCCCGATCGCGAAGAACACCGCTGGCTCCGTGAAGAACAACGATGGCTTCGCGAGGAGCAACGGTGGATTCGCGAGGAGAATCGATGGAATCGTGAACGGGATGAGCTTCTCAGAGAGATTTCCGAACTTAAACTTCAAATTCAATCCCTCGAGCATCGAATTATATCTTCATCACCTTCTTCTTCTACGTCTTCCGATGCGGTTTCGAATGTTGCGGCGTTGCTACAGGTGTTGAAGGATAAGAATTTGGTTCTTGAGAGTGGTTCTAGTCAGAGGAGGTTAGTGCTTGAGGAGGAAAAGGAAGATGAAGTGGAAGAAGAAAAGGAATCTGTTGAGGTTGTAAAGGAGATTGTGGTTGTTGAGAAACCTGCTGCTAAGGTTGAAAAGAGGACTGTTTTGCGAACAGGTTCTGAAGGAGAAGAGGTTCAAAAGTTGCAGGAAGCATTGCTAAAATTGGGATTTTACTCGGGTGAGGAGGACATGGAATTCTCTAGCTTCTCAAGTGGGACTGAGCGTGCTGTGAAGACTTGGCAATCTTCGTTGGGCATCCCTGAGGATGGGATCATGACGTCTAATCTTCTTGAGAGGCTATATTTGGATATAAGGACTACGGATATAGATAGTGTAAATGAAACCAAAAAATCTGCTAATGTTTTACCGAAGGAAGTTGAAAATGGAGCTGCAGTTGCTTCCGTGACAGAAATTTCAGAGGTTCAGCAAAAAGTCGTGGGAGAAGTTGACCAAGAAACAGAAGCATCCCATCCAAGAGTTTTCCTCCTTGGGGAAAATCGGTGGGAAGAACCATCAAGACTTTCGACGAAGGTTAGAGTTGATAGGGGCAAGAACAAGGACGGAACAATGAAATGCCTTCAATGTTCTGGGCAAGGTATCTTGTTGTGTACAGAATGTGATGGATCTGGTGAGCCAAATATTGAACCTCAAGTAAGGGATAATTGTGCTTTTTATGAAAATTAA
Protein sequence

>MsG0180004006.01.T01

MSLSTPTVPITVTVTLNSTLSFPTKTLSFLKPHFSSYKFNSFTAHCSIPDREEHRWLREEQRWLREEQRWIREENRWNRERDELLREISELKLQIQSLEHRIISSSPSSSTSSDAVSNVAALLQVLKDKNLVLESGSSQRRLVLEEEKEDEVEEEKESVEVVKEIVVVEKPAAKVEKRTVLRTGSEGEEVQKLQEALLKLGFYSGEEDMEFSSFSSGTERAVKTWQSSLGIPEDGIMTSNLLERLYLDIRTTDIDSVNETKKSANVLPKEVENGAAVASVTEISEVQQKVVGEVDQETEASHPRVFLLGENRWEEPSRLSTKVRVDRGKNKDGTMKCLQCSGQGILLCTECDGSGEPNIEPQVRDNCAFYEN*