Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043092.01.T01 | XP_013444749.1 | 98.78 | 82 | 1 | 0 | 1 | 82 | 173 | 254 | 1.21E-52 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043092.01.T01 | A0A072TMQ2 | 98.780 | 82 | 1 | 0 | 1 | 82 | 173 | 254 | 5.79e-53 | 174 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049069.01 | MsG0880043092.01 | 0.814096 | 1.833271e-51 | 1.218736e-48 |
| MsG0080049070.01 | MsG0880043092.01 | 0.815079 | 1.110819e-51 | 7.583425e-49 |
| MsG0180001548.01 | MsG0880043092.01 | 0.843791 | 1.127559e-58 | 1.783764e-55 |
| MsG0180004006.01 | MsG0880043092.01 | 0.810880 | 9.251017e-51 | 5.642836e-48 |
| MsG0180005964.01 | MsG0880043092.01 | 0.827697 | 1.355507e-54 | 1.318457e-51 |
| MsG0880042987.01 | MsG0880043092.01 | -0.800386 | 1.476850e-48 | 6.867624e-46 |
| MsG0280010985.01 | MsG0880043092.01 | 0.800473 | 1.417708e-48 | 6.606887e-46 |
| MsG0380012868.01 | MsG0880043092.01 | 0.808383 | 3.181215e-50 | 1.816421e-47 |
| MsG0680032850.01 | MsG0880043092.01 | 0.824614 | 7.335150e-54 | 6.531243e-51 |
| MsG0480019812.01 | MsG0880043092.01 | 0.820189 | 7.824544e-53 | 6.148723e-50 |
| MsG0480021038.01 | MsG0880043092.01 | 0.831764 | 1.387275e-55 | 1.520278e-52 |
| MsG0480021213.01 | MsG0880043092.01 | 0.806796 | 6.913458e-50 | 3.786994e-47 |
| MsG0480022113.01 | MsG0880043092.01 | 0.836113 | 1.131580e-56 | 1.412226e-53 |
| MsG0480022162.01 | MsG0880043092.01 | 0.804290 | 2.319085e-49 | 1.191191e-46 |
| MsG0580025627.01 | MsG0880043092.01 | 0.804362 | 2.240533e-49 | 1.152980e-46 |
| MsG0380015024.01 | MsG0880043092.01 | 0.824745 | 6.834529e-54 | 6.108185e-51 |
| MsG0380017427.01 | MsG0880043092.01 | 0.856181 | 3.826690e-62 | 9.054924e-59 |
| MsG0380017793.01 | MsG0880043092.01 | 0.819101 | 1.386161e-52 | 1.056772e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043092.01.T01 | MTR_8g028425 | 98.780 | 82 | 1 | 0 | 1 | 82 | 173 | 254 | 1.47e-56 | 174 |
| MsG0880043092.01.T01 | MTR_2g064370 | 56.944 | 72 | 29 | 1 | 2 | 73 | 160 | 229 | 5.17e-26 | 97.1 |
| MsG0880043092.01.T01 | MTR_8g046460 | 54.795 | 73 | 30 | 2 | 1 | 72 | 159 | 229 | 1.14e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043092.01.T01 | AT2G46220 | 70.667 | 75 | 22 | 0 | 1 | 75 | 165 | 239 | 1.75e-39 | 130 |
| MsG0880043092.01.T01 | AT1G79510 | 53.425 | 73 | 31 | 2 | 1 | 72 | 165 | 235 | 2.21e-24 | 92.4 |
| MsG0880043092.01.T01 | AT1G79510 | 53.425 | 73 | 31 | 2 | 1 | 72 | 165 | 235 | 2.21e-24 | 92.4 |
| MsG0880043092.01.T01 | AT1G16320 | 52.703 | 74 | 33 | 2 | 1 | 73 | 163 | 235 | 4.07e-23 | 89.4 |
Find 27 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGCCGCGGTAGGTTTGA+TGG | 0.435547 | 8:+18984334 | MsG0880043092.01.T01:CDS |
| ATGGTTCGCTGGACGGTTCA+CGG | 0.441932 | 8:+18984292 | MsG0880043092.01.T01:CDS |
| TGGTTCGCTGGACGGTTCAC+GGG | 0.442263 | 8:+18984293 | MsG0880043092.01.T01:CDS |
| ATTTCAAAGTAAGTTGGTCC+TGG | 0.452612 | 8:-18984493 | None:intergenic |
| GAAGAATTGATTCAATCTCT+TGG | 0.484168 | 8:+18984457 | MsG0880043092.01.T01:CDS |
| CTTGGCTGTCCGCAGACCCC+AGG | 0.492505 | 8:+18984475 | MsG0880043092.01.T01:CDS |
| TTTCAAAGTAAGTTGGTCCT+GGG | 0.497418 | 8:-18984492 | None:intergenic |
| GAAGATATTTCAAAGTAAGT+TGG | 0.497988 | 8:-18984499 | None:intergenic |
| CGGCTTTCCCATGGAACTCG+TGG | 0.505084 | 8:-18984319 | None:intergenic |
| AGGCAAGATTTACGAACATC+GGG | 0.513299 | 8:+18984384 | MsG0880043092.01.T01:CDS |
| ACGGGATCCCACGAGTTCCA+TGG | 0.525112 | 8:+18984311 | MsG0880043092.01.T01:CDS |
| AACCTACCGCGGCTTTCCCA+TGG | 0.527295 | 8:-18984328 | None:intergenic |
| CGGGATCCCACGAGTTCCAT+GGG | 0.531141 | 8:+18984312 | MsG0880043092.01.T01:CDS |
| ATGTAATATGGTTCGCTGGA+CGG | 0.536091 | 8:+18984285 | None:intergenic |
| ACTGACATCACTTTAAACCT+AGG | 0.556783 | 8:-18984433 | None:intergenic |
| GGCTTTCCCATGGAACTCGT+GGG | 0.560830 | 8:-18984318 | None:intergenic |
| AAGGCAAGATTTACGAACAT+CGG | 0.568854 | 8:+18984383 | MsG0880043092.01.T01:CDS |
| TTTGTCAAGTTTATACTCAG+AGG | 0.572049 | 8:-18984359 | None:intergenic |
| GACATCACTTTAAACCTAGG+AGG | 0.582014 | 8:-18984430 | None:intergenic |
| TAAGTTGGTCCTGGGGTCTG+CGG | 0.605321 | 8:-18984484 | None:intergenic |
| TTCAAAGTAAGTTGGTCCTG+GGG | 0.626091 | 8:-18984491 | None:intergenic |
| GAGAATGTAATATGGTTCGC+TGG | 0.628696 | 8:+18984281 | None:intergenic |
| TAGGTTTAAAGTGATGTCAG+TGG | 0.634165 | 8:+18984435 | MsG0880043092.01.T01:CDS |
| TTCCATGGGAAAGCCGCGGT+AGG | 0.661780 | 8:+18984326 | MsG0880043092.01.T01:CDS |
| GAGTATAAACTTGACAAACA+AGG | 0.672357 | 8:+18984364 | MsG0880043092.01.T01:CDS |
| CGAGTTCCATGGGAAAGCCG+CGG | 0.685486 | 8:+18984322 | MsG0880043092.01.T01:CDS |
| AGGTTCCATCAAACCTACCG+CGG | 0.698339 | 8:-18984339 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGATATTTCAAAGTAAGT+TGG | - | Chr8:18984502-18984521 | None:intergenic | 25.0% |
| GAGTATAAACTTGACAAACA+AGG | + | Chr8:18984364-18984383 | MsG0880043092.01.T01:CDS | 30.0% | |
| TTTGTCAAGTTTATACTCAG+AGG | - | Chr8:18984362-18984381 | None:intergenic | 30.0% | |
| ! | GAAGAATTGATTCAATCTCT+TGG | + | Chr8:18984457-18984476 | MsG0880043092.01.T01:CDS | 30.0% |
| AAGGCAAGATTTACGAACAT+CGG | + | Chr8:18984383-18984402 | MsG0880043092.01.T01:CDS | 35.0% | |
| ACTGACATCACTTTAAACCT+AGG | - | Chr8:18984436-18984455 | None:intergenic | 35.0% | |
| ! | TAGGTTTAAAGTGATGTCAG+TGG | + | Chr8:18984435-18984454 | MsG0880043092.01.T01:CDS | 35.0% |
| !! | ATTTCAAAGTAAGTTGGTCC+TGG | - | Chr8:18984496-18984515 | None:intergenic | 35.0% |
| !! | TTGCTTTTAACAAACCTCCT+AGG | + | Chr8:18984416-18984435 | MsG0880043092.01.T01:CDS | 35.0% |
| !! | TTTCAAAGTAAGTTGGTCCT+GGG | - | Chr8:18984495-18984514 | None:intergenic | 35.0% |
| AGGCAAGATTTACGAACATC+GGG | + | Chr8:18984384-18984403 | MsG0880043092.01.T01:CDS | 40.0% | |
| GACATCACTTTAAACCTAGG+AGG | - | Chr8:18984433-18984452 | None:intergenic | 40.0% | |
| !! | TTCAAAGTAAGTTGGTCCTG+GGG | - | Chr8:18984494-18984513 | None:intergenic | 40.0% |
| AGGTTCCATCAAACCTACCG+CGG | - | Chr8:18984342-18984361 | None:intergenic | 50.0% | |
| ATGGTTCGCTGGACGGTTCA+CGG | + | Chr8:18984292-18984311 | MsG0880043092.01.T01:CDS | 55.0% | |
| GAAAGCCGCGGTAGGTTTGA+TGG | + | Chr8:18984334-18984353 | MsG0880043092.01.T01:CDS | 55.0% | |
| GGCTTTCCCATGGAACTCGT+GGG | - | Chr8:18984321-18984340 | None:intergenic | 55.0% | |
| !! | TAAGTTGGTCCTGGGGTCTG+CGG | - | Chr8:18984487-18984506 | None:intergenic | 55.0% |
| AACCTACCGCGGCTTTCCCA+TGG | - | Chr8:18984331-18984350 | None:intergenic | 60.0% | |
| ACGGGATCCCACGAGTTCCA+TGG | + | Chr8:18984311-18984330 | MsG0880043092.01.T01:CDS | 60.0% | |
| CGAGTTCCATGGGAAAGCCG+CGG | + | Chr8:18984322-18984341 | MsG0880043092.01.T01:CDS | 60.0% | |
| CGGCTTTCCCATGGAACTCG+TGG | - | Chr8:18984322-18984341 | None:intergenic | 60.0% | |
| CGGGATCCCACGAGTTCCAT+GGG | + | Chr8:18984312-18984331 | MsG0880043092.01.T01:CDS | 60.0% | |
| TGGTTCGCTGGACGGTTCAC+GGG | + | Chr8:18984293-18984312 | MsG0880043092.01.T01:CDS | 60.0% | |
| TTCCATGGGAAAGCCGCGGT+AGG | + | Chr8:18984326-18984345 | MsG0880043092.01.T01:CDS | 60.0% | |
| CTTGGCTGTCCGCAGACCCC+AGG | + | Chr8:18984475-18984494 | MsG0880043092.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 18984292 | 18984540 | 18984292 | ID=MsG0880043092.01;Name=MsG0880043092.01 |
| Chr8 | mRNA | 18984292 | 18984540 | 18984292 | ID=MsG0880043092.01.T01;Parent=MsG0880043092.01;Name=MsG0880043092.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|82 |
| Chr8 | exon | 18984292 | 18984540 | 18984292 | ID=MsG0880043092.01.T01:exon:3767;Parent=MsG0880043092.01.T01 |
| Chr8 | CDS | 18984292 | 18984540 | 18984292 | ID=MsG0880043092.01.T01:cds;Parent=MsG0880043092.01.T01 |
| Gene Sequence |
| Protein sequence |
