AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017427.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017427.01.T01 MTR_3g107397 60.000 80 8 1 1 56 61 140 5.89e-24 87.0
MsG0380017427.01.T01 MTR_4g114180 40.000 80 23 2 1 55 53 132 1.01e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017427.01.T01 AT3G47650 43.750 80 21 1 1 56 57 136 4.46e-16 67.0

Find 20 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATGAAGTATGTAATAA+TGG 0.243773 3:+96172526 None:intergenic
TCAGCACAGATGACACTATT+TGG 0.263290 3:+96173377 None:intergenic
TGCAATGGAGCTGGATTTAT+CGG 0.322631 3:-96172796 MsG0380017427.01.T01:CDS
TCAATGAAGTATGTAATAAT+GGG 0.409734 3:+96172527 None:intergenic
TTATTACATACTTCATTGAA+TGG 0.423113 3:-96172523 None:intergenic
TTGGATCTGTCCCTCCCTAC+TGG 0.458506 3:+96172752 None:intergenic
CAGTTCACTTGTAGCCATAG+TGG 0.465843 3:-96172638 MsG0380017427.01.T01:three_prime_UTR
CTGAACTTGTTCGCAGTCGT+TGG 0.486092 3:+96172733 None:intergenic
TGTGGGAATTGCAATGGAGC+TGG 0.521634 3:-96172805 MsG0380017427.01.T01:CDS
GGCAGGAAGGAGATGTTATG+TGG 0.530714 3:-96172823 MsG0380017427.01.T01:CDS
TGTGCTGATTGTGATGGAAA+TGG 0.538508 3:-96173362 MsG0380017427.01.T01:CDS
GCAGGAAGGAGATGTTATGT+GGG 0.542628 3:-96172822 MsG0380017427.01.T01:CDS
CTTGAGCACATATGACCAGT+AGG 0.554604 3:-96172767 MsG0380017427.01.T01:exon
ATGTTATGTGGGAATTGCAA+TGG 0.609174 3:-96172811 MsG0380017427.01.T01:CDS
AATGGAGCTGGATTTATCGG+TGG 0.609254 3:-96172793 MsG0380017427.01.T01:CDS
TTGAGCACATATGACCAGTA+GGG 0.613108 3:-96172766 MsG0380017427.01.T01:exon
GTCATCTGTGCTGATTGTGA+TGG 0.623087 3:-96173368 MsG0380017427.01.T01:CDS
AGCACATATGACCAGTAGGG+AGG 0.624035 3:-96172763 MsG0380017427.01.T01:exon
CACTATCATACTGATGCTCA+AGG 0.627147 3:-96172585 MsG0380017427.01.T01:three_prime_UTR
GCACATATGACCAGTAGGGA+GGG 0.642431 3:-96172762 MsG0380017427.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATAAAAAAATGC+AGG - Chr3:96173322-96173341 MsG0380017427.01.T01:intron 10.0%
!! AAAATTTGCATTTAACAATT+AGG + Chr3:96172967-96172986 None:intergenic 15.0%
!!! ATAGAATGATTTTACTAATT+AGG + Chr3:96172907-96172926 None:intergenic 15.0%
!!! TTATATTTTGTGTAATTAAC+AGG - Chr3:96172782-96172801 MsG0380017427.01.T01:CDS 15.0%
!! CGATGAACAATAATAATATA+AGG + Chr3:96173074-96173093 None:intergenic 20.0%
!! TCAATGAAGTATGTAATAAT+GGG + Chr3:96173407-96173426 None:intergenic 20.0%
!! TTATTACATACTTCATTGAA+TGG - Chr3:96173408-96173427 MsG0380017427.01.T01:CDS 20.0%
!! TTCAATGAAGTATGTAATAA+TGG + Chr3:96173408-96173427 None:intergenic 20.0%
!!! AGATGACATATGATTTTATA+AGG - Chr3:96172594-96172613 MsG0380017427.01.T01:three_prime_UTR 20.0%
! CAAGAGAAAATATACATCTA+CGG - Chr3:96172746-96172765 MsG0380017427.01.T01:three_prime_UTR 25.0%
! TAATAATATAAGGCACATTG+TGG + Chr3:96173064-96173083 None:intergenic 25.0%
!!! TATTTGGTTTTGTGTTTTGT+TGG + Chr3:96172541-96172560 None:intergenic 25.0%
!!! TTTTTTTTTAGCAACCACTA+TGG + Chr3:96173310-96173329 None:intergenic 25.0%
AACGATTTACATGCTCATAA+CGG + Chr3:96172672-96172691 None:intergenic 30.0%
AATGTATTTGTGGTTGTTGA+AGG - Chr3:96172717-96172736 MsG0380017427.01.T01:three_prime_UTR 30.0%
TGCTTCTTGTAATGTATTTG+TGG - Chr3:96172707-96172726 MsG0380017427.01.T01:three_prime_UTR 30.0%
! AACTCTGTTGATATTTTCGA+TGG - Chr3:96172842-96172861 MsG0380017427.01.T01:intron 30.0%
AAGGCTTCATTGTATTCTAG+AGG + Chr3:96173002-96173021 None:intergenic 35.0%
ATGTTATGTGGGAATTGCAA+TGG - Chr3:96173120-96173139 MsG0380017427.01.T01:intron 35.0%
! AATGAAGCCTTATGATCAGT+AGG - Chr3:96173011-96173030 MsG0380017427.01.T01:intron 35.0%
! ATGAAGCCTTATGATCAGTA+GGG - Chr3:96173012-96173031 MsG0380017427.01.T01:intron 35.0%
!!! TTCATCGTTTTTTACTCCAG+AGG - Chr3:96173087-96173106 MsG0380017427.01.T01:intron 35.0%
ATTGTCTGCTCTCAATGCAA+AGG - Chr3:96172809-96172828 MsG0380017427.01.T01:CDS 40.0%
CACTATCATACTGATGCTCA+AGG - Chr3:96173346-96173365 MsG0380017427.01.T01:CDS 40.0%
TCAGCACAGATGACACTATT+TGG + Chr3:96172557-96172576 None:intergenic 40.0%
TGCAATGGAGCTGGATTTAT+CGG - Chr3:96173135-96173154 MsG0380017427.01.T01:intron 40.0%
TTGAGCACATATGACCAGTA+GGG - Chr3:96173165-96173184 MsG0380017427.01.T01:intron 40.0%
!! TGTGCTGATTGTGATGGAAA+TGG - Chr3:96172569-96172588 MsG0380017427.01.T01:three_prime_UTR 40.0%
!! TTCGATGGACTGTTTAAAGC+TGG - Chr3:96172857-96172876 MsG0380017427.01.T01:intron 40.0%
AATGGAGCTGGATTTATCGG+TGG - Chr3:96173138-96173157 MsG0380017427.01.T01:intron 45.0%
CAGTTCACTTGTAGCCATAG+TGG - Chr3:96173293-96173312 MsG0380017427.01.T01:intron 45.0%
CTTGAGCACATATGACCAGT+AGG - Chr3:96173164-96173183 MsG0380017427.01.T01:intron 45.0%
GCAGGAAGGAGATGTTATGT+GGG - Chr3:96173109-96173128 MsG0380017427.01.T01:intron 45.0%
GTTCCTCCCTACTGATCATA+AGG + Chr3:96173021-96173040 None:intergenic 45.0%
TGCTCTCAATGCAAAGGTAG+CGG - Chr3:96172815-96172834 MsG0380017427.01.T01:CDS 45.0%
! AAGCCTTATGATCAGTAGGG+AGG - Chr3:96173015-96173034 MsG0380017427.01.T01:intron 45.0%
! TTTTTACTCCAGAGGCAGGA+AGG - Chr3:96173095-96173114 MsG0380017427.01.T01:intron 45.0%
!! GTCATCTGTGCTGATTGTGA+TGG - Chr3:96172563-96172582 MsG0380017427.01.T01:three_prime_UTR 45.0%
!!! TCGTTTTTTACTCCAGAGGC+AGG - Chr3:96173091-96173110 MsG0380017427.01.T01:intron 45.0%
AGCACATATGACCAGTAGGG+AGG - Chr3:96173168-96173187 MsG0380017427.01.T01:intron 50.0%
GCACATATGACCAGTAGGGA+GGG - Chr3:96173169-96173188 MsG0380017427.01.T01:intron 50.0%
GGCAGGAAGGAGATGTTATG+TGG - Chr3:96173108-96173127 MsG0380017427.01.T01:intron 50.0%
TAACATCTCCTTCCTGCCTC+TGG + Chr3:96173106-96173125 None:intergenic 50.0%
TGTGGGAATTGCAATGGAGC+TGG - Chr3:96173126-96173145 MsG0380017427.01.T01:intron 50.0%
TTAAAGCTGGTGCCTCCTGT+TGG - Chr3:96172870-96172889 MsG0380017427.01.T01:intron 50.0%
! CTGAACTTGTTCGCAGTCGT+TGG + Chr3:96173201-96173220 None:intergenic 50.0%
TAGGTACCCGCAAAGCCAAC+AGG + Chr3:96172888-96172907 None:intergenic 55.0%
TTGGATCTGTCCCTCCCTAC+TGG + Chr3:96173182-96173201 None:intergenic 55.0%
GTACCCGCAAAGCCAACAGG+AGG + Chr3:96172885-96172904 None:intergenic 60.0%
!! GGTGCCTCCTGTTGGCTTTG+CGG - Chr3:96172878-96172897 MsG0380017427.01.T01:intron 60.0%
!! GTGCCTCCTGTTGGCTTTGC+GGG - Chr3:96172879-96172898 MsG0380017427.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 96172524 96173429 96172524 ID=MsG0380017427.01;Name=MsG0380017427.01
Chr3 mRNA 96172524 96173429 96172524 ID=MsG0380017427.01.T01;Parent=MsG0380017427.01;Name=MsG0380017427.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0.5|1|0|0|2|244|56
Chr3 exon 96172524 96172846 96172524 ID=MsG0380017427.01.T01:exon:17451;Parent=MsG0380017427.01.T01
Chr3 exon 96173338 96173429 96173338 ID=MsG0380017427.01.T01:exon:17450;Parent=MsG0380017427.01.T01
Chr3 CDS 96173338 96173429 96173338 ID=MsG0380017427.01.T01:cds;Parent=MsG0380017427.01.T01
Chr3 CDS 96172768 96172846 96172768 ID=MsG0380017427.01.T01:cds;Parent=MsG0380017427.01.T01
Chr3 three_prime_UTR 96172524 96172767 96172524 ID=MsG0380017427.01.T01:three_prime_utr;Parent=MsG0380017427.01.T01
Gene Sequence

>MsG0380017427.01.T01

GCTACAAATGACAACCAACAAAACACAAAACCAAATAGTGTCATCTGTGCTGATTGTGATGGAAATGGTAAGATGACATATGATTTTATAAGAGGCAGGAAGGAGATGTTATGTGGGAATTGCAATGGAGCTGGATTTATCGGTGGCTTCTTGAGCACATATGACCAGTAG

Protein sequence

>MsG0380017427.01.T01

ATNDNQQNTKPNSVICADCDGNGKMTYDFIRGRKEMLCGNCNGAGFIGGFLSTYDQ*