AlfalfaGEDB | MsG0480021038.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021038.01

KEGG: -
GO: GO:0016021 GO:0022857 GO:0055085
PFAM: PF00083

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021038.01.T01	KEH30181.1	100	81	0	0	1	81	1	81	6.15E-48	156

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021038.01.T01	Q0WWW9	85.185	81	12	0	1	81	478	558	3.17E-40	141

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021038.01.T01	A0A072ULA2	100.000	81	0	0	1	81	1	81	2.94e-48	156

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048995.01	MsG0480021038.01	0.835313	1.804225e-56	2.198333e-53
MsG0080049069.01	MsG0480021038.01	0.829769	4.276580e-55	4.420044e-52
MsG0080049070.01	MsG0480021038.01	0.887916	9.366604e-73	7.102104e-69
MsG0180000549.01	MsG0480021038.01	0.845437	4.064493e-59	6.773001e-56
MsG0180000833.01	MsG0480021038.01	0.824059	9.909163e-54	8.682460e-51
MsG0180000834.01	MsG0480021038.01	0.817915	2.574540e-52	1.899569e-49
MsG0180001548.01	MsG0480021038.01	0.907360	5.512941e-81	9.747332e-77
MsG0180002700.01	MsG0480021038.01	0.818289	2.118281e-52	1.579057e-49
MsG0180003196.01	MsG0480021038.01	0.859737	3.361851e-63	8.970542e-60
MsG0180003637.01	MsG0480021038.01	-0.839743	1.320123e-57	1.840093e-54
MsG0180004006.01	MsG0480021038.01	0.834751	2.499915e-56	2.994263e-53
MsG0180004121.01	MsG0480021038.01	-0.800744	1.248376e-48	5.858009e-46
MsG0180004792.01	MsG0480021038.01	0.841272	5.255318e-58	7.681446e-55
MsG0180004954.01	MsG0480021038.01	-0.831834	1.333182e-55	1.463982e-52
MsG0180004979.01	MsG0480021038.01	0.844024	9.766643e-59	1.556517e-55
MsG0180005395.01	MsG0480021038.01	-0.805247	1.463557e-49	7.704882e-47
MsG0180005636.01	MsG0480021038.01	-0.804865	1.759658e-49	9.174006e-47
MsG0180005666.01	MsG0480021038.01	0.811698	6.145282e-51	3.829984e-48
MsG0180005822.01	MsG0480021038.01	0.831936	1.258467e-55	1.386090e-52
MsG0180005964.01	MsG0480021038.01	0.846952	1.571962e-59	2.750847e-56
MsG0180006028.01	MsG0480021038.01	0.851933	6.427129e-61	1.321327e-57
MsG0180006217.01	MsG0480021038.01	0.835547	1.574836e-56	1.931991e-53
MsG0280011218.01	MsG0480021038.01	0.820367	7.122420e-53	5.624582e-50
MsG0380012658.01	MsG0480021038.01	-0.815654	8.277683e-52	5.741187e-49
MsG0380012868.01	MsG0480021038.01	0.858402	8.442476e-63	2.152121e-59
MsG0480018676.01	MsG0480021038.01	0.809386	1.941317e-50	1.138272e-47
MsG0480019644.01	MsG0480021038.01	0.805981	1.026878e-49	5.509160e-47
MsG0480019812.01	MsG0480021038.01	0.898453	5.228316e-77	6.183849e-73
MsG0480020473.01	MsG0480021038.01	0.854949	8.751118e-62	1.987708e-58
MsG0480020596.01	MsG0480021038.01	0.859051	5.404272e-63	1.408235e-59
MsG0480021038.01	MsG0480021101.01	0.819426	1.169006e-52	8.992849e-50
MsG0480021038.01	MsG0480021154.01	-0.810996	8.728346e-51	5.340980e-48
MsG0480021038.01	MsG0480021213.01	0.881003	3.444115e-70	1.983904e-66
MsG0480021038.01	MsG0480021967.01	0.836642	8.303555e-57	1.052749e-53
MsG0480021038.01	MsG0480022113.01	0.877029	8.716170e-69	4.317802e-65
MsG0480021038.01	MsG0480022154.01	0.808035	3.774951e-50	2.135589e-47
MsG0480021038.01	MsG0480022162.01	0.878328	3.069887e-69	1.596317e-65
MsG0480021038.01	MsG0480023406.01	0.812809	3.517003e-51	2.257934e-48
MsG0480021038.01	MsG0480023454.01	-0.816710	4.805253e-52	3.430307e-49
MsG0480021038.01	MsG0480023466.01	0.847513	1.103108e-59	1.965449e-56
MsG0480021038.01	MsG0480024011.01	0.825712	4.036437e-54	3.708026e-51
MsG0480021038.01	MsG0580025610.01	0.808156	3.556547e-50	2.018605e-47
MsG0480021038.01	MsG0580025627.01	0.822293	2.559875e-53	2.133846e-50
MsG0480021038.01	MsG0580027183.01	0.883519	4.187856e-71	2.663903e-67
MsG0480021038.01	MsG0580027189.01	0.876853	1.003321e-68	4.939638e-65
MsG0480021038.01	MsG0580029325.01	0.809308	2.017674e-50	1.180524e-47
MsG0480021038.01	MsG0580029511.01	0.845329	4.347619e-59	7.220441e-56
MsG0480021038.01	MsG0580029760.01	0.819845	9.374564e-53	7.296536e-50
MsG0480021038.01	MsG0680031826.01	0.875549	2.823531e-68	1.323866e-64
MsG0480021038.01	MsG0680032850.01	0.877129	8.045160e-69	4.000791e-65
MsG0480021038.01	MsG0680035532.01	0.826246	3.014528e-54	2.811701e-51
MsG0480021038.01	MsG0780035965.01	0.833254	5.927987e-56	6.789224e-53
MsG0480021038.01	MsG0780035976.01	-0.824624	7.296710e-54	6.498871e-51
MsG0480021038.01	MsG0780037351.01	0.804943	1.694421e-49	8.851808e-47
MsG0480021038.01	MsG0780037363.01	0.827058	1.929340e-54	1.841992e-51
MsG0480021038.01	MsG0780037507.01	0.823659	1.229812e-53	1.065634e-50
MsG0480021038.01	MsG0780038056.01	0.802725	4.895915e-49	2.415965e-46
MsG0480021038.01	MsG0780039161.01	0.831968	1.235244e-55	1.361751e-52
MsG0480021038.01	MsG0780040214.01	0.800304	1.534602e-48	7.121549e-46
MsG0480021038.01	MsG0780040236.01	0.829623	4.641687e-55	4.776348e-52
MsG0480021038.01	MsG0780040550.01	0.827175	1.809211e-54	1.733288e-51
MsG0480021038.01	MsG0780040684.01	0.825941	3.561895e-54	3.293667e-51
MsG0480021038.01	MsG0780040965.01	0.821114	4.795447e-53	3.866448e-50
MsG0480021038.01	MsG0780041174.01	0.805932	1.051161e-49	5.632375e-47
MsG0480021038.01	MsG0780041331.01	0.880426	5.541627e-70	3.121355e-66
MsG0480021038.01	MsG0780041361.01	0.859114	5.175110e-63	1.351354e-59
MsG0480021038.01	MsG0780041474.01	0.803830	2.890688e-49	1.467368e-46
MsG0480021038.01	MsG0880041847.01	0.868100	8.390473e-66	2.998514e-62
MsG0480021038.01	MsG0880042086.01	0.824418	8.158088e-54	7.221428e-51
MsG0480021038.01	MsG0880042415.01	0.862256	5.765309e-64	1.677692e-60
MsG0480021038.01	MsG0880043024.01	0.812433	4.249642e-51	2.701855e-48
MsG0480021038.01	MsG0880043092.01	0.831764	1.387275e-55	1.520278e-52
MsG0480021038.01	MsG0880043476.01	0.833805	4.318172e-56	5.028214e-53
MsG0480021038.01	MsG0880045312.01	0.811188	7.932488e-51	4.877918e-48
MsG0480021038.01	MsG0880045327.01	0.806873	6.658669e-50	3.654740e-47
MsG0480021038.01	MsG0880045648.01	0.800106	1.683813e-48	7.774458e-46
MsG0380016057.01	MsG0480021038.01	0.834922	2.265138e-56	2.727403e-53
MsG0380016172.01	MsG0480021038.01	0.825076	5.709483e-54	5.151013e-51
MsG0380017162.01	MsG0480021038.01	0.826403	2.765065e-54	2.590682e-51
MsG0380017427.01	MsG0480021038.01	0.919506	4.132896e-87	1.331906e-82
MsG0380017793.01	MsG0480021038.01	0.813087	3.056972e-51	1.977235e-48
MsG0480018107.01	MsG0480021038.01	0.802329	5.908121e-49	2.886192e-46
MsG0480018337.01	MsG0480021038.01	0.909199	7.411202e-82	1.424749e-77
MsG0480018338.01	MsG0480021038.01	0.877692	5.124141e-69	2.601281e-65
MsG0280006505.01	MsG0480021038.01	-0.822862	1.887346e-53	1.598917e-50
MsG0280007406.01	MsG0480021038.01	0.905563	3.762932e-80	6.120989e-76
MsG0280007647.01	MsG0480021038.01	0.817685	2.900523e-52	2.126675e-49
MsG0280007852.01	MsG0480021038.01	0.844106	9.284150e-59	1.483422e-55
MsG0280007853.01	MsG0480021038.01	0.857621	1.440489e-62	3.577567e-59
MsG0280008223.01	MsG0480021038.01	0.801264	9.774467e-49	4.647508e-46
MsG0280008343.01	MsG0480021038.01	0.817000	4.137268e-52	2.977269e-49
MsG0280008452.01	MsG0480021038.01	-0.821219	4.536481e-53	3.668471e-50
MsG0280010468.01	MsG0480021038.01	-0.840568	8.037794e-58	1.149549e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021038.01.T01	MTR_4g064807	100.000	81	0	0	1	81	1	81	7.45e-52	156
MsG0480021038.01.T01	MTR_4g077740	71.250	80	23	0	1	80	422	501	2.04e-31	114
MsG0480021038.01.T01	MTR_2g026160	46.835	79	42	0	3	81	480	558	3.36e-15	68.9
MsG0480021038.01.T01	MTR_5g075300	44.000	75	42	0	3	77	406	480	4.63e-14	65.9
MsG0480021038.01.T01	MTR_4g072030	42.667	75	43	0	3	77	411	485	8.76e-14	64.7
MsG0480021038.01.T01	MTR_2g049020	38.667	75	46	0	3	77	477	551	1.39e-12	61.6
MsG0480021038.01.T01	MTR_7g446340	39.474	76	46	0	1	76	424	499	2.56e-12	60.8
MsG0480021038.01.T01	MTR_6g007340	39.474	76	46	0	1	76	424	499	2.56e-12	60.8
MsG0480021038.01.T01	MTR_3g116240	37.975	79	49	0	3	81	415	493	4.09e-12	60.1
MsG0480021038.01.T01	MTR_5g077580	39.241	79	47	1	3	81	474	551	1.11e-11	58.9
MsG0480021038.01.T01	MTR_4g118610	38.961	77	46	1	1	77	409	484	1.34e-11	58.5
MsG0480021038.01.T01	MTR_4g069500	36.709	79	50	0	3	81	365	443	2.14e-11	58.2
MsG0480021038.01.T01	MTR_4g069500	36.709	79	50	0	3	81	415	493	2.24e-11	58.2
MsG0480021038.01.T01	MTR_4g069500	36.709	79	50	0	3	81	226	304	2.26e-11	57.8
MsG0480021038.01.T01	MTR_8g077890	40.541	74	44	0	3	76	406	479	2.58e-11	57.8
MsG0480021038.01.T01	MTR_1g116650	39.189	74	45	0	3	76	436	509	3.06e-11	57.8
MsG0480021038.01.T01	MTR_3g116060	37.333	75	47	0	1	75	639	713	3.40e-11	57.4
MsG0480021038.01.T01	MTR_7g094110	36.709	79	49	1	1	79	398	475	6.32e-11	56.6
MsG0480021038.01.T01	MTR_5g024740	40.000	75	45	0	1	75	631	705	7.72e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021038.01.T01	AT5G59250	85.185	81	12	0	1	81	478	558	3.23e-41	141
MsG0480021038.01.T01	AT3G03090	71.250	80	23	0	1	80	424	503	7.74e-31	112
MsG0480021038.01.T01	AT5G17010	70.000	80	24	0	1	80	424	503	3.93e-30	110
MsG0480021038.01.T01	AT5G17010	70.000	80	24	0	1	80	424	503	3.93e-30	110
MsG0480021038.01.T01	AT5G17010	70.000	80	24	0	1	80	424	503	3.93e-30	110
MsG0480021038.01.T01	AT5G17010	67.500	80	26	0	1	80	361	440	9.05e-29	106
MsG0480021038.01.T01	AT4G16480	47.297	74	39	0	3	76	484	557	9.45e-16	70.5
MsG0480021038.01.T01	AT4G16480	47.297	74	39	0	3	76	484	557	9.45e-16	70.5
MsG0480021038.01.T01	AT4G16480	47.297	74	39	0	3	76	484	557	9.45e-16	70.5
MsG0480021038.01.T01	AT1G30220	43.243	74	42	0	3	76	480	553	1.96e-14	66.6
MsG0480021038.01.T01	AT2G35740	47.297	74	39	0	3	76	483	556	5.56e-14	65.5
MsG0480021038.01.T01	AT3G18830	41.892	74	43	0	3	76	422	495	1.33e-13	64.3
MsG0480021038.01.T01	AT2G16130	44.000	75	42	0	3	77	413	487	1.81e-13	63.9
MsG0480021038.01.T01	AT5G27360	38.272	81	49	1	1	81	287	366	6.32e-13	62.4
MsG0480021038.01.T01	AT5G27360	38.272	81	49	1	1	81	287	366	6.32e-13	62.4
MsG0480021038.01.T01	AT5G27360	38.272	81	49	1	1	81	439	518	6.78e-13	62.4
MsG0480021038.01.T01	AT5G27360	38.272	81	49	1	1	81	391	470	7.00e-13	62.4
MsG0480021038.01.T01	AT5G27350	40.741	81	47	1	1	81	387	466	1.06e-12	62.0
MsG0480021038.01.T01	AT2G18480	43.243	74	42	0	3	76	407	480	1.85e-12	61.2
MsG0480021038.01.T01	AT2G16120	40.789	76	45	0	2	77	412	487	1.85e-12	61.2
MsG0480021038.01.T01	AT1G08900	33.333	81	53	1	1	81	339	418	3.89e-12	60.1
MsG0480021038.01.T01	AT1G08900	33.333	81	53	1	1	81	373	452	4.04e-12	60.1
MsG0480021038.01.T01	AT1G08900	33.333	81	53	1	1	81	381	460	4.38e-12	60.1
MsG0480021038.01.T01	AT1G08900	33.333	81	53	1	1	81	381	460	4.38e-12	60.1
MsG0480021038.01.T01	AT4G36670	42.667	75	43	0	3	77	403	477	5.61e-12	59.7
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	451	525	6.35e-12	59.7
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	648	722	1.44e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	638	712	1.70e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	638	712	1.70e-11	58.5
MsG0480021038.01.T01	AT4G35300	38.667	75	46	0	1	75	638	712	1.70e-11	58.5
MsG0480021038.01.T01	AT1G20840	36.000	75	48	0	1	75	641	715	2.00e-11	58.2
MsG0480021038.01.T01	AT1G20840	36.000	75	48	0	1	75	641	715	2.00e-11	58.2
MsG0480021038.01.T01	AT5G18840	35.802	81	51	1	1	81	401	480	3.12e-11	57.8
MsG0480021038.01.T01	AT2G48020	37.662	77	47	1	1	77	384	459	4.79e-11	57.0
MsG0480021038.01.T01	AT2G48020	37.662	77	47	1	1	77	384	459	4.79e-11	57.0
MsG0480021038.01.T01	AT3G05165	36.709	79	49	1	1	79	386	463	4.98e-11	57.0
MsG0480021038.01.T01	AT3G05165	36.709	79	49	1	1	79	386	463	4.98e-11	57.0
MsG0480021038.01.T01	AT3G05165	36.709	79	49	1	1	79	386	463	4.98e-11	57.0

Find 23 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGCTGTCACTTCAAGATTT+TGG	0.110124	4:+52479765	None:intergenic
CTAATGCAATGGCCGCAAAA+AGG	0.369183	4:+52479854	None:intergenic
CTTTGCATTCTCACCATTGA+AGG	0.377650	4:-52480016	MsG0480021038.01.T01:intron
ATTCCACTGCAGGAGTATCT+TGG	0.414752	4:-52479895	MsG0480021038.01.T01:intron
CAAGTTACTCAAATCACATC+AGG	0.433338	4:-52479626	MsG0480021038.01.T01:three_prime_UTR
TATGATACATTCACATCAAA+AGG	0.439235	4:+52479734	None:intergenic
AACAGCATTGGCAGCGAAGT+TGG	0.454377	4:+52480044	None:intergenic
CCCACTTCGCACTAGAGGAC+GGG	0.489293	4:-52480088	MsG0480021038.01.T01:CDS
ATTGTATATATCACATGTGT+GGG	0.524514	4:-52479702	MsG0480021038.01.T01:three_prime_UTR
CTGATGTGATTTGAGTAACT+TGG	0.529187	4:+52479627	None:intergenic
ACTTCTGTCCCAGAGACAAA+AGG	0.535446	4:-52479811	MsG0480021038.01.T01:CDS
TGCAAAGGTAACAACAGCAT+TGG	0.547945	4:+52480032	None:intergenic
CCCGTCCTCTAGTGCGAAGT+GGG	0.561592	4:+52480088	None:intergenic
CCACTTCGCACTAGAGGACG+GGG	0.565068	4:-52480087	MsG0480021038.01.T01:CDS
TCCCACTTCGCACTAGAGGA+CGG	0.571506	4:-52480089	MsG0480021038.01.T01:CDS
CATTGTATATATCACATGTG+TGG	0.573595	4:-52479703	MsG0480021038.01.T01:three_prime_UTR
ATCTTCCCACTTCGCACTAG+AGG	0.577398	4:-52480093	MsG0480021038.01.T01:CDS
CACAAGTGACACTAATGCAA+TGG	0.600703	4:+52479843	None:intergenic
CCCCGTCCTCTAGTGCGAAG+TGG	0.606766	4:+52480087	None:intergenic
CTTCAATGGTGAGAATGCAA+AGG	0.636883	4:+52480017	None:intergenic
TTCTTCGTGTAATGAACTGA+AGG	0.640507	4:+52479501	None:intergenic
AAGTTACTCAAATCACATCA+GGG	0.687402	4:-52479625	MsG0480021038.01.T01:three_prime_UTR
CACTTCGCACTAGAGGACGG+GGG	0.710664	4:-52480086	MsG0480021038.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTCAATAAGAAAATAAAAC+TGG	+	Chr4:52479641-52479660	None:intergenic	20.0%
!	AGAAAATAAAACTGGAAGTA+AGG	+	Chr4:52479633-52479652	None:intergenic	25.0%
!	ATTGTATATATCACATGTGT+GGG	-	Chr4:52479912-52479931	MsG0480021038.01.T01:intron	25.0%
!	TATGATACATTCACATCAAA+AGG	+	Chr4:52479883-52479902	None:intergenic	25.0%
	AAGGAATCAAAAACCTTCAA+TGG	+	Chr4:52479614-52479633	None:intergenic	30.0%
	AAGTTACTCAAATCACATCA+GGG	-	Chr4:52479989-52480008	MsG0480021038.01.T01:intron	30.0%
	CATTGTATATATCACATGTG+TGG	-	Chr4:52479911-52479930	MsG0480021038.01.T01:intron	30.0%
	TAAATTTGTCTGAATGCTGT+AGG	-	Chr4:52479677-52479696	MsG0480021038.01.T01:three_prime_UTR	30.0%
	CAAGTTACTCAAATCACATC+AGG	-	Chr4:52479988-52480007	MsG0480021038.01.T01:intron	35.0%
	CTGATGTGATTTGAGTAACT+TGG	+	Chr4:52479990-52480009	None:intergenic	35.0%
!	GAACCTTTTTCTCCTTTTTG+CGG	-	Chr4:52479748-52479767	MsG0480021038.01.T01:three_prime_UTR	35.0%
	CACAAGTGACACTAATGCAA+TGG	+	Chr4:52479774-52479793	None:intergenic	40.0%
	CTTCAATGGTGAGAATGCAA+AGG	+	Chr4:52479600-52479619	None:intergenic	40.0%
	CTTTGCATTCTCACCATTGA+AGG	-	Chr4:52479598-52479617	MsG0480021038.01.T01:three_prime_UTR	40.0%
	TGCAAAGGTAACAACAGCAT+TGG	+	Chr4:52479585-52479604	None:intergenic	40.0%
!!	CTGCTGTCACTTCAAGATTT+TGG	+	Chr4:52479852-52479871	None:intergenic	40.0%
	ACTTCTGTCCCAGAGACAAA+AGG	-	Chr4:52479803-52479822	MsG0480021038.01.T01:CDS	45.0%
	CTAATGCAATGGCCGCAAAA+AGG	+	Chr4:52479763-52479782	None:intergenic	45.0%
	TCTGTGTTGAATTCCACTGC+AGG	-	Chr4:52479709-52479728	MsG0480021038.01.T01:three_prime_UTR	45.0%
	TGGCCGCAAAAAGGAGAAAA+AGG	+	Chr4:52479754-52479773	None:intergenic	45.0%
!	AAGCTCAAGCCTTTTGTCTC+TGG	+	Chr4:52479815-52479834	None:intergenic	45.0%
!	AGCTCAAGCCTTTTGTCTCT+GGG	+	Chr4:52479814-52479833	None:intergenic	45.0%
!!	ATTCCACTGCAGGAGTATCT+TGG	-	Chr4:52479719-52479738	MsG0480021038.01.T01:three_prime_UTR	45.0%
	ATCTTCCCACTTCGCACTAG+AGG	-	Chr4:52479521-52479540	MsG0480021038.01.T01:three_prime_UTR	50.0%
!	TAGAGGACGGGGGATTAGTA+TGG	-	Chr4:52479538-52479557	MsG0480021038.01.T01:three_prime_UTR	50.0%
!!	AACAGCATTGGCAGCGAAGT+TGG	+	Chr4:52479573-52479592	None:intergenic	50.0%
	GCTCCAAGATACTCCTGCAG+TGG	+	Chr4:52479725-52479744	None:intergenic	55.0%
	TCCCACTTCGCACTAGAGGA+CGG	-	Chr4:52479525-52479544	MsG0480021038.01.T01:three_prime_UTR	55.0%
	CACTTCGCACTAGAGGACGG+GGG	-	Chr4:52479528-52479547	MsG0480021038.01.T01:three_prime_UTR	60.0%
	CCACTTCGCACTAGAGGACG+GGG	-	Chr4:52479527-52479546	MsG0480021038.01.T01:three_prime_UTR	60.0%
	CCCACTTCGCACTAGAGGAC+GGG	-	Chr4:52479526-52479545	MsG0480021038.01.T01:three_prime_UTR	60.0%
	CCCGTCCTCTAGTGCGAAGT+GGG	+	Chr4:52479529-52479548	None:intergenic	60.0%
	CCCCGTCCTCTAGTGCGAAG+TGG	+	Chr4:52479530-52479549	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	52479509	52480127	52479509	ID=MsG0480021038.01;Name=MsG0480021038.01
Chr4	mRNA	52479509	52480127	52479509	ID=MsG0480021038.01.T01;Parent=MsG0480021038.01;Name=MsG0480021038.01.T01;_AED=0.22;_eAED=0.22;_QI=0\|1\|1\|1\|0.07\|0.07\|14\|262\|81
Chr4	exon	52479509	52479905	52479509	ID=MsG0480021038.01.T01:exon:842;Parent=MsG0480021038.01.T01
Chr4	exon	52480017	52480127	52480017	ID=MsG0480021038.01.T01:exon:841;Parent=MsG0480021038.01.T01
Chr4	CDS	52480017	52480127	52480017	ID=MsG0480021038.01.T01:cds;Parent=MsG0480021038.01.T01
Chr4	CDS	52479771	52479905	52479771	ID=MsG0480021038.01.T01:cds;Parent=MsG0480021038.01.T01
Chr4	three_prime_UTR	52479509	52479770	52479509	ID=MsG0480021038.01.T01:three_prime_utr;Parent=MsG0480021038.01.T01

Gene Sequence	>MsG0480021038.01.T01 ATGGTATCTGAAATCTTCCCACTTCGCACTAGAGGACGGGGGATTAGTATGGCTGTTCTTACCAACTTCGCTGCCAATGCTGTTGTTACCTTTGCATTCTCACCATTGAAGGAGTATCTTGGAGCAGAGAACCTTTTTCTCCTTTTTGCGGCCATTGCATTAGTGTCACTTGTGTTCATTGTAACTTCTGTCCCAGAGACAAAAGGCTTGAGCTTAGAAGAAATTGAATCCAAAATCTTGAAGTGA
Protein sequence	>MsG0480021038.01.T01 MVSEIFPLRTRGRGISMAVLTNFAANAVVTFAFSPLKEYLGAENLFLLFAAIALVSLVFIVTSVPETKGLSLEEIESKILK*