AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004098.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004098.01.T01 MTR_1g075170 79.554 269 36 2 1 251 1 268 1.40e-144 420
MsG0180004098.01.T01 MTR_1g075153 51.587 252 108 4 12 250 13 263 3.25e-74 238
MsG0180004098.01.T01 MTR_1g075180 44.770 239 117 4 27 252 7 243 2.89e-57 193
MsG0180004098.01.T01 MTR_1g040360 35.424 271 136 6 19 251 13 282 1.03e-34 131
MsG0180004098.01.T01 MTR_8g063020 42.484 153 70 2 83 234 49 184 2.22e-26 105
MsG0180004098.01.T01 MTR_1g074950 31.835 267 134 7 31 252 21 284 1.02e-24 102
MsG0180004098.01.T01 MTR_1g074990 27.820 266 142 6 31 250 20 281 3.11e-23 98.6
MsG0180004098.01.T01 MTR_1g075095 26.592 267 146 6 31 250 20 283 3.22e-20 89.7
MsG0180004098.01.T01 MTR_1g075030 24.907 269 148 7 31 250 20 283 2.51e-18 84.3
MsG0180004098.01.T01 MTR_1g075230 25.379 264 151 7 31 250 20 281 3.07e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004098.01.T01 AT3G01680 30.882 136 83 3 89 216 123 255 1.56e-11 64.3

Find 62 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACTTGTCACATTCTTAT+TGG 0.141753 1:+73342497 MsG0180004098.01.T01:CDS
CTTGGGAGGTACCATTCTTT+TGG 0.182584 1:-73341679 None:intergenic
ATCAATATCGTCAAGCTTAA+AGG 0.190937 1:-73341726 None:intergenic
GTGCAATTACGTATCTTAAT+AGG 0.248309 1:+73342394 MsG0180004098.01.T01:CDS
GCCATTGATTGGTACATCTT+TGG 0.251729 1:-73341648 None:intergenic
GAAAGTTGCAAAGTGGAAAT+TGG 0.271554 1:+73342847 MsG0180004098.01.T01:CDS
TCTGCCTCAACTCAGATTTC+TGG 0.271962 1:+73341836 MsG0180004098.01.T01:CDS
AACTCTAGCTGCATTCTCTT+TGG 0.285355 1:+73342365 MsG0180004098.01.T01:CDS
GCTGCATTCTCTTTGGAGTA+TGG 0.291746 1:+73342372 MsG0180004098.01.T01:CDS
GGAGCTGAGATTTCTGTTCT+TGG 0.330206 1:-73341697 None:intergenic
TCGTCAAGCTTAAAGGGATT+AGG 0.343557 1:-73341719 None:intergenic
TAACGAGTTGAGGCCTCATT+TGG 0.343868 1:+73342825 MsG0180004098.01.T01:CDS
GAGCTGAGATTTCTGTTCTT+GGG 0.379012 1:-73341696 None:intergenic
CGTCAAGCTTAAAGGGATTA+GGG 0.380837 1:-73341718 None:intergenic
GGATTCCTCTTGTTGTGTAT+TGG 0.381172 1:+73342610 MsG0180004098.01.T01:CDS
ATCACCTCAGAGACTTCTCC+TGG 0.410904 1:+73342325 MsG0180004098.01.T01:CDS
TGGGTCGTAGTGCCATTGAT+TGG 0.424583 1:-73341659 None:intergenic
TGGGCAGCATGGTCAGCTTT+CGG 0.428459 1:+73342543 MsG0180004098.01.T01:CDS
GCTACCATTGTTGCCTGTAC+TGG 0.428950 1:+73342639 MsG0180004098.01.T01:CDS
GGGTGATATAAACTTTGTTA+AGG 0.430161 1:-73341751 None:intergenic
TCAATATCGTCAAGCTTAAA+GGG 0.433331 1:-73341725 None:intergenic
GTCAGCTTTCGGTTATGACT+TGG 0.459956 1:+73342554 MsG0180004098.01.T01:CDS
TGTTGGAGACAAGCTTGAAA+AGG 0.463060 1:-73341808 None:intergenic
CACCCATGTCAAAGATTATG+AGG 0.466569 1:+73341769 MsG0180004098.01.T01:CDS
GCCTGTACTGGTAACCTTGT+CGG 0.472360 1:+73342651 MsG0180004098.01.T01:CDS
TGCACATTCTGCTGTGCCGC+GGG 0.492853 1:-73342277 None:intergenic
GAACCTTTCTGAATTGAACT+TGG 0.497414 1:-73342461 None:intergenic
ACTTTGCAACTTTCCAAATG+AGG 0.497491 1:-73342838 None:intergenic
ACCGACAAGGTTACCAGTAC+AGG 0.500684 1:-73342652 None:intergenic
GGAATCCATTGCATAGCATC+AGG 0.501216 1:-73342594 None:intergenic
AAATTAATAAGATTTGATAC+AGG 0.501510 1:-73341592 None:intergenic
TCCAAAGATGTACCAATCAA+TGG 0.506446 1:+73341647 MsG0180004098.01.T01:CDS
GTTGGAGACAAGCTTGAAAA+GGG 0.511266 1:-73341807 None:intergenic
AGGAGAAGTCTCTGAGGTGA+TGG 0.514175 1:-73342323 None:intergenic
TAGCCAAGTTCAATTCAGAA+AGG 0.519066 1:+73342458 MsG0180004098.01.T01:CDS
GGAGAATCCACATTGTTGTC+TGG 0.519858 1:-73342302 None:intergenic
AGTTGAGGCAGAGAGGATGT+TGG 0.533320 1:-73341825 None:intergenic
GGCACTACGACCCAAAAGAA+TGG 0.536228 1:+73341668 MsG0180004098.01.T01:CDS
CTGAGATTTCTGTTCTTGGG+AGG 0.538881 1:-73341693 None:intergenic
ACAAGTTCAGTTATATCAGC+AGG 0.544593 1:-73342483 None:intergenic
TTCCTCATAATCTTTGACAT+GGG 0.560884 1:-73341771 None:intergenic
ATTCCTCATAATCTTTGACA+TGG 0.565424 1:-73341772 None:intergenic
GTGCACATTCTGCTGTGCCG+CGG 0.581489 1:-73342278 None:intergenic
CTTTGCCTGATGCTATGCAA+TGG 0.584464 1:+73342589 MsG0180004098.01.T01:CDS
ACTGTCCAATACACAACAAG+AGG 0.585978 1:-73342615 None:intergenic
ATGTGGTGGTTAACGAGTTG+AGG 0.588601 1:+73342815 MsG0180004098.01.T01:CDS
AGTTCCAGAAATCTGAGTTG+AGG 0.604866 1:-73341840 None:intergenic
TACATCTTTGGACAATGCAG+TGG 0.608358 1:-73341636 None:intergenic
ACATTAAACAGTGGGCAGCA+TGG 0.610506 1:+73342532 MsG0180004098.01.T01:CDS
ATTCAATCATCAGACACACT+TGG 0.616321 1:+73342417 MsG0180004098.01.T01:CDS
TTTGGACAATGCAGTGGACA+TGG 0.617395 1:-73341630 None:intergenic
TCATTTGGAAAGTTGCAAAG+TGG 0.618816 1:+73342840 MsG0180004098.01.T01:CDS
GTTACCAGTACAGGCAACAA+TGG 0.619153 1:-73342643 None:intergenic
AAATCTGAGTTGAGGCAGAG+AGG 0.625519 1:-73341832 None:intergenic
GTTACAAGACATTAAACAGT+GGG 0.627387 1:+73342524 MsG0180004098.01.T01:CDS
TCACCTCAGAGACTTCTCCT+GGG 0.638066 1:+73342326 MsG0180004098.01.T01:CDS
CACCTAGCAGAAATTAAGCA+AGG 0.644336 1:+73341546 None:intergenic
CTTCCCAGGAGAAGTCTCTG+AGG 0.675866 1:-73342329 None:intergenic
TGTTACAAGACATTAAACAG+TGG 0.717621 1:+73342523 MsG0180004098.01.T01:CDS
TGACAGATGATAACGACCCG+CGG 0.734185 1:+73342261 MsG0180004098.01.T01:intron
GTGCACACCAGACAACAATG+TGG 0.739193 1:+73342295 MsG0180004098.01.T01:CDS
GAACTTACGATATACCGACA+AGG 0.746980 1:-73342665 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATCAGATTATAAAAAA+AGG + Chr1:73342785-73342804 MsG0180004098.01.T01:CDS 10.0%
!! AAATTAATAAGATTTGATAC+AGG - Chr1:73341595-73341614 None:intergenic 15.0%
!! ATAAAAAAAAAAAACTCCAT+CGG + Chr1:73342104-73342123 MsG0180004098.01.T01:intron 15.0%
!! TATTAAAATGTATAAGTGAA+AGG - Chr1:73341893-73341912 None:intergenic 15.0%
!!! TTTTATTAATCTGTATGAAA+TGG + Chr1:73342179-73342198 MsG0180004098.01.T01:intron 15.0%
!! TATATACATGTAAGTGTAAA+TGG + Chr1:73342737-73342756 MsG0180004098.01.T01:intron 20.0%
! AAATTATCCTTATTAACCGA+TGG - Chr1:73342123-73342142 None:intergenic 25.0%
! ATGTATATACGATATAGTGA+CGG - Chr1:73342726-73342745 None:intergenic 25.0%
!! AATGGTTCTTTTATTCTTGT+AGG + Chr1:73342755-73342774 MsG0180004098.01.T01:intron 25.0%
!! ATGGTTCTTTTATTCTTGTA+GGG + Chr1:73342756-73342775 MsG0180004098.01.T01:intron 25.0%
ATCAATATCGTCAAGCTTAA+AGG - Chr1:73341729-73341748 None:intergenic 30.0%
ATTCCTCATAATCTTTGACA+TGG - Chr1:73341775-73341794 None:intergenic 30.0%
GGGTGATATAAACTTTGTTA+AGG - Chr1:73341754-73341773 None:intergenic 30.0%
GTGCAATTACGTATCTTAAT+AGG + Chr1:73342394-73342413 MsG0180004098.01.T01:CDS 30.0%
GTTACAAGACATTAAACAGT+GGG + Chr1:73342524-73342543 MsG0180004098.01.T01:CDS 30.0%
TCAATATCGTCAAGCTTAAA+GGG - Chr1:73341728-73341747 None:intergenic 30.0%
TGAACTTGTCACATTCTTAT+TGG + Chr1:73342497-73342516 MsG0180004098.01.T01:CDS 30.0%
TGTTACAAGACATTAAACAG+TGG + Chr1:73342523-73342542 MsG0180004098.01.T01:CDS 30.0%
TTCCTCATAATCTTTGACAT+GGG - Chr1:73341774-73341793 None:intergenic 30.0%
! AAAAACTCCATCGGTTAATA+AGG + Chr1:73342113-73342132 MsG0180004098.01.T01:intron 30.0%
ACAAGTTCAGTTATATCAGC+AGG - Chr1:73342486-73342505 None:intergenic 35.0%
ACTTTGCAACTTTCCAAATG+AGG - Chr1:73342841-73342860 None:intergenic 35.0%
ATTCAATCATCAGACACACT+TGG + Chr1:73342417-73342436 MsG0180004098.01.T01:CDS 35.0%
GAAAGTTGCAAAGTGGAAAT+TGG + Chr1:73342847-73342866 MsG0180004098.01.T01:CDS 35.0%
GAACCTTTCTGAATTGAACT+TGG - Chr1:73342464-73342483 None:intergenic 35.0%
GATAGACGAAAGAATCAAGA+TGG - Chr1:73342156-73342175 None:intergenic 35.0%
TAAAAAAAGGCTCTCTCATG+TGG + Chr1:73342798-73342817 MsG0180004098.01.T01:CDS 35.0%
TAGCCAAGTTCAATTCAGAA+AGG + Chr1:73342458-73342477 MsG0180004098.01.T01:CDS 35.0%
TCATTTGGAAAGTTGCAAAG+TGG + Chr1:73342840-73342859 MsG0180004098.01.T01:CDS 35.0%
TCCAAAGATGTACCAATCAA+TGG + Chr1:73341647-73341666 MsG0180004098.01.T01:CDS 35.0%
! GTCAGGTTAGTTGTTAATGT+CGG + Chr1:73342043-73342062 MsG0180004098.01.T01:intron 35.0%
AACTCTAGCTGCATTCTCTT+TGG + Chr1:73342365-73342384 MsG0180004098.01.T01:CDS 40.0%
ACTGTCCAATACACAACAAG+AGG - Chr1:73342618-73342637 None:intergenic 40.0%
AGTTCCAGAAATCTGAGTTG+AGG - Chr1:73341843-73341862 None:intergenic 40.0%
CACCCATGTCAAAGATTATG+AGG + Chr1:73341769-73341788 MsG0180004098.01.T01:CDS 40.0%
CGTCAAGCTTAAAGGGATTA+GGG - Chr1:73341721-73341740 None:intergenic 40.0%
GAACTTACGATATACCGACA+AGG - Chr1:73342668-73342687 None:intergenic 40.0%
GAGCTGAGATTTCTGTTCTT+GGG - Chr1:73341699-73341718 None:intergenic 40.0%
GTTGGAGACAAGCTTGAAAA+GGG - Chr1:73341810-73341829 None:intergenic 40.0%
TACATCTTTGGACAATGCAG+TGG - Chr1:73341639-73341658 None:intergenic 40.0%
TCGTCAAGCTTAAAGGGATT+AGG - Chr1:73341722-73341741 None:intergenic 40.0%
TGTTGGAGACAAGCTTGAAA+AGG - Chr1:73341811-73341830 None:intergenic 40.0%
! GCCATTGATTGGTACATCTT+TGG - Chr1:73341651-73341670 None:intergenic 40.0%
! GGATTCCTCTTGTTGTGTAT+TGG + Chr1:73342610-73342629 MsG0180004098.01.T01:CDS 40.0%
! TATCAATGCTTTTGCTTCCC+AGG - Chr1:73342346-73342365 None:intergenic 40.0%
!!! TTGGGAGGTACCATTCTTTT+GGG - Chr1:73341681-73341700 None:intergenic 40.0%
AAAAAGGCTCTCTCATGTGG+TGG + Chr1:73342801-73342820 MsG0180004098.01.T01:CDS 45.0%
ACATTAAACAGTGGGCAGCA+TGG + Chr1:73342532-73342551 MsG0180004098.01.T01:CDS 45.0%
ATGTGGTGGTTAACGAGTTG+AGG + Chr1:73342815-73342834 MsG0180004098.01.T01:CDS 45.0%
CCTGAAGAGGATTTCTTGTC+AGG + Chr1:73342026-73342045 MsG0180004098.01.T01:intron 45.0%
CCTGACAAGAAATCCTCTTC+AGG - Chr1:73342029-73342048 None:intergenic 45.0%
CTGAGATTTCTGTTCTTGGG+AGG - Chr1:73341696-73341715 None:intergenic 45.0%
CTTTGCCTGATGCTATGCAA+TGG + Chr1:73342589-73342608 MsG0180004098.01.T01:CDS 45.0%
GGAATCCATTGCATAGCATC+AGG - Chr1:73342597-73342616 None:intergenic 45.0%
GGAGAATCCACATTGTTGTC+TGG - Chr1:73342305-73342324 None:intergenic 45.0%
GGAGCTGAGATTTCTGTTCT+TGG - Chr1:73341700-73341719 None:intergenic 45.0%
GTCAGCTTTCGGTTATGACT+TGG + Chr1:73342554-73342573 MsG0180004098.01.T01:CDS 45.0%
GTTACCAGTACAGGCAACAA+TGG - Chr1:73342646-73342665 None:intergenic 45.0%
TCTGCCTCAACTCAGATTTC+TGG + Chr1:73341836-73341855 MsG0180004098.01.T01:CDS 45.0%
TTTGGACAATGCAGTGGACA+TGG - Chr1:73341633-73341652 None:intergenic 45.0%
! AAATCTGAGTTGAGGCAGAG+AGG - Chr1:73341835-73341854 None:intergenic 45.0%
! TAACGAGTTGAGGCCTCATT+TGG + Chr1:73342825-73342844 MsG0180004098.01.T01:CDS 45.0%
!! GCTGCATTCTCTTTGGAGTA+TGG + Chr1:73342372-73342391 MsG0180004098.01.T01:CDS 45.0%
!!! CTTGGGAGGTACCATTCTTT+TGG - Chr1:73341682-73341701 None:intergenic 45.0%
ACCGACAAGGTTACCAGTAC+AGG - Chr1:73342655-73342674 None:intergenic 50.0%
AGTTGAGGCAGAGAGGATGT+TGG - Chr1:73341828-73341847 None:intergenic 50.0%
ATCACCTCAGAGACTTCTCC+TGG + Chr1:73342325-73342344 MsG0180004098.01.T01:CDS 50.0%
CAGACTTCTCTGTCCTGAAG+AGG + Chr1:73342013-73342032 MsG0180004098.01.T01:intron 50.0%
CTCTTCAGGACAGAGAAGTC+TGG - Chr1:73342015-73342034 None:intergenic 50.0%
GCTACCATTGTTGCCTGTAC+TGG + Chr1:73342639-73342658 MsG0180004098.01.T01:CDS 50.0%
GGCACTACGACCCAAAAGAA+TGG + Chr1:73341668-73341687 MsG0180004098.01.T01:CDS 50.0%
GTGCACACCAGACAACAATG+TGG + Chr1:73342295-73342314 MsG0180004098.01.T01:CDS 50.0%
TCACCTCAGAGACTTCTCCT+GGG + Chr1:73342326-73342345 MsG0180004098.01.T01:CDS 50.0%
! GCCTGTACTGGTAACCTTGT+CGG + Chr1:73342651-73342670 MsG0180004098.01.T01:CDS 50.0%
! TGACAGATGATAACGACCCG+CGG + Chr1:73342261-73342280 MsG0180004098.01.T01:intron 50.0%
! TGGGTCGTAGTGCCATTGAT+TGG - Chr1:73341662-73341681 None:intergenic 50.0%
!! AGGAGAAGTCTCTGAGGTGA+TGG - Chr1:73342326-73342345 None:intergenic 50.0%
CTTCCCAGGAGAAGTCTCTG+AGG - Chr1:73342332-73342351 None:intergenic 55.0%
TGGGCAGCATGGTCAGCTTT+CGG + Chr1:73342543-73342562 MsG0180004098.01.T01:CDS 55.0%
GTGCACATTCTGCTGTGCCG+CGG - Chr1:73342281-73342300 None:intergenic 60.0%
TGCACATTCTGCTGTGCCGC+GGG - Chr1:73342280-73342299 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 73341561 73342876 73341561 ID=MsG0180004098.01;Name=MsG0180004098.01
Chr1 mRNA 73341561 73342876 73341561 ID=MsG0180004098.01.T01;Parent=MsG0180004098.01;Name=MsG0180004098.01.T01;_AED=0.49;_eAED=0.50;_QI=71|0.5|0.66|1|1|1|3|0|252
Chr1 exon 73341561 73341877 73341561 ID=MsG0180004098.01.T01:exon:5260;Parent=MsG0180004098.01.T01
Chr1 exon 73342267 73342679 73342267 ID=MsG0180004098.01.T01:exon:5261;Parent=MsG0180004098.01.T01
Chr1 exon 73342777 73342876 73342777 ID=MsG0180004098.01.T01:exon:5262;Parent=MsG0180004098.01.T01
Chr1 five_prime_UTR 73341561 73341631 73341561 ID=MsG0180004098.01.T01:five_prime_utr;Parent=MsG0180004098.01.T01
Chr1 CDS 73341632 73341877 73341632 ID=MsG0180004098.01.T01:cds;Parent=MsG0180004098.01.T01
Chr1 CDS 73342267 73342679 73342267 ID=MsG0180004098.01.T01:cds;Parent=MsG0180004098.01.T01
Chr1 CDS 73342777 73342876 73342777 ID=MsG0180004098.01.T01:cds;Parent=MsG0180004098.01.T01
Gene Sequence

>MsG0180004098.01.T01

ATGTCCACTGCATTGTCCAAAGATGTACCAATCAATGGCACTACGACCCAAAAGAATGGTACCTCCCAAGAACAGAAATCTCAGCTCCCTAATCCCTTTAAGCTTGACGATATTGATATCCTTAACAAAGTTTATATCACCCATGTCAAAGATTATGAGGAATATGATAATGAAACCCTTTTCAAGCTTGTCTCCAACATCCTCTCTGCCTCAACTCAGATTTCTGGAACTAATTCTGCACTTAACATGATAACGACCCGCGGCACAGCAGAATGTGCACACCAGACAACAATGTGGATTCTCCATCACCTCAGAGACTTCTCCTGGGAAGCAAAAGCATTGATAACTCTAGCTGCATTCTCTTTGGAGTATGGTGCAATTACGTATCTTAATAGGATTCAATCATCAGACACACTTGGAAACTCATTGAAGCAACTTAGCCAAGTTCAATTCAGAAAGGTTCCTGCTGATATAACTGAACTTGTCACATTCTTATTGGATGTGTTACAAGACATTAAACAGTGGGCAGCATGGTCAGCTTTCGGTTATGACTTGGACGCTGTTCATTCTTTGCCTGATGCTATGCAATGGATTCCTCTTGTTGTGTATTGGACAGTTGCTACCATTGTTGCCTGTACTGGTAACCTTGTCGGTATATCGGAGCATATATTATCAGATTATAAAAAAAGGCTCTCTCATGTGGTGGTTAACGAGTTGAGGCCTCATTTGGAAAGTTGCAAAGTGGAAATTGGTACATAA

Protein sequence

>MsG0180004098.01.T01

MSTALSKDVPINGTTTQKNGTSQEQKSQLPNPFKLDDIDILNKVYITHVKDYEEYDNETLFKLVSNILSASTQISGTNSALNMITTRGTAECAHQTTMWILHHLRDFSWEAKALITLAAFSLEYGAITYLNRIQSSDTLGNSLKQLSQVQFRKVPADITELVTFLLDVLQDIKQWAAWSAFGYDLDAVHSLPDAMQWIPLVVYWTVATIVACTGNLVGISEHILSDYKKRLSHVVVNELRPHLESCKVEIGT*