AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003779.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003779.01.T01 MTR_1g069465 99.375 160 1 0 11 170 23 182 1.35e-118 333
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003779.01.T01 AT4G28660 68.966 145 45 0 25 169 37 181 8.60e-72 214
MsG0180003779.01.T01 AT4G28660 68.966 145 45 0 25 169 52 196 1.77e-71 214

Find 38 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGAATGAATTGAATTT+TGG 0.043355 1:+68113853 None:intergenic
GGAGAAGTAGGCGACATTAC+TGG 0.365446 1:-68113725 MsG0180003779.01.T01:CDS
CCCAAAATTCAATTCATTCA+AGG 0.375868 1:-68113854 MsG0180003779.01.T01:CDS
AACTTAAATTCCTCATTCAA+TGG 0.412356 1:-68113965 MsG0180003779.01.T01:CDS
TTCCCCGCCATCATGATAAC+CGG 0.429760 1:+68113878 None:intergenic
TGGAGTCCGCATTATATACT+TGG 0.434350 1:+68113616 None:intergenic
CAAATGGCATGGCTATCTTC+AGG 0.434591 1:-68113778 MsG0180003779.01.T01:CDS
ATTTGCATGTGCCAAGTTTG+AGG 0.436765 1:-68113937 MsG0180003779.01.T01:CDS
TAATGCGGACTCCACGGGAC+TGG 0.442232 1:-68113607 MsG0180003779.01.T01:CDS
AAATCAAAGGATGGAACAAA+TGG 0.447952 1:-68113794 MsG0180003779.01.T01:CDS
TTTGATTCTTCTGGAGAAGT+AGG 0.453284 1:-68113737 MsG0180003779.01.T01:CDS
GGATAGGTTCATGAGATTCA+TGG 0.460311 1:-68113585 MsG0180003779.01.T01:CDS
CATTTACATCAACAGATTGA+AGG 0.465513 1:+68113675 None:intergenic
GCCACTTCTTTAATTATCCA+TGG 0.476745 1:-68113995 MsG0180003779.01.T01:CDS
GAGCGATACTCTGATGCTAA+TGG 0.480616 1:-68113563 MsG0180003779.01.T01:CDS
AATTTAAGTTCAAACTTCCA+TGG 0.492114 1:+68113978 None:intergenic
CATGATAACCGGCATGTGCA+TGG 0.501061 1:+68113889 None:intergenic
ATGCCGGTTATCATGATGGC+GGG 0.504435 1:-68113881 MsG0180003779.01.T01:CDS
AATGCGGACTCCACGGGACT+GGG 0.524053 1:-68113606 MsG0180003779.01.T01:CDS
GGACTCCACGGGACTGGGAT+AGG 0.532501 1:-68113601 MsG0180003779.01.T01:CDS
GTATATAATGCGGACTCCAC+GGG 0.541871 1:-68113612 MsG0180003779.01.T01:CDS
GCACATGCCGGTTATCATGA+TGG 0.545209 1:-68113885 MsG0180003779.01.T01:CDS
ATACTTGGCTTCAATTCCTG+AGG 0.546032 1:+68113631 None:intergenic
ATCATCGGTAACCTCAAACT+TGG 0.546137 1:+68113926 None:intergenic
ATGATAACCGGCATGTGCAT+GGG 0.547496 1:+68113890 None:intergenic
TCCATGGATAATTAAAGAAG+TGG 0.555425 1:+68113994 None:intergenic
TTTGTCAACGGAAAGCCCTC+AGG 0.559746 1:-68113647 MsG0180003779.01.T01:CDS
AGTATATAATGCGGACTCCA+CGG 0.565595 1:-68113613 MsG0180003779.01.T01:CDS
TACTTGGCTTCAATTCCTGA+GGG 0.571164 1:+68113632 None:intergenic
AAGCTGACAAAATCAAAGGA+TGG 0.597295 1:-68113803 MsG0180003779.01.T01:CDS
GTAAATGCTAAATTTGTCAA+CGG 0.597700 1:-68113659 MsG0180003779.01.T01:CDS
CAAATGTTGACCATTGAATG+AGG 0.613868 1:+68113955 None:intergenic
TGCCGGTTATCATGATGGCG+GGG 0.623175 1:-68113880 MsG0180003779.01.T01:CDS
AAAGGATGGAACAAATGGCA+TGG 0.632758 1:-68113789 MsG0180003779.01.T01:CDS
CATGCCGGTTATCATGATGG+CGG 0.658092 1:-68113882 MsG0180003779.01.T01:CDS
TGTGAAGCTGACAAAATCAA+AGG 0.662632 1:-68113807 MsG0180003779.01.T01:CDS
ATGAACCTATCCCAGTCCCG+TGG 0.667895 1:+68113596 None:intergenic
TTGAAGCCAAGTATATAATG+CGG 0.690031 1:-68113622 MsG0180003779.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTAAATTCCTCATTCAA+TGG - Chr1:68113607-68113626 MsG0180003779.01.T01:CDS 25.0%
! GTAAATGCTAAATTTGTCAA+CGG - Chr1:68113913-68113932 MsG0180003779.01.T01:CDS 25.0%
!! AATTTAAGTTCAAACTTCCA+TGG + Chr1:68113597-68113616 None:intergenic 25.0%
!! CCTTGAATGAATTGAATTTT+GGG + Chr1:68113721-68113740 None:intergenic 25.0%
!! TCCTTGAATGAATTGAATTT+TGG + Chr1:68113722-68113741 None:intergenic 25.0%
!! TGGTTTTTATATGATTGATG+AGG - Chr1:68113867-68113886 MsG0180003779.01.T01:CDS 25.0%
!! TTTTATATGATTGATGAGGA+AGG - Chr1:68113871-68113890 MsG0180003779.01.T01:CDS 25.0%
AAATCAAAGGATGGAACAAA+TGG - Chr1:68113778-68113797 MsG0180003779.01.T01:CDS 30.0%
CATTTACATCAACAGATTGA+AGG + Chr1:68113900-68113919 None:intergenic 30.0%
CCCAAAATTCAATTCATTCA+AGG - Chr1:68113718-68113737 MsG0180003779.01.T01:CDS 30.0%
TCCATGGATAATTAAAGAAG+TGG + Chr1:68113581-68113600 None:intergenic 30.0%
TTGAAGCCAAGTATATAATG+CGG - Chr1:68113950-68113969 MsG0180003779.01.T01:CDS 30.0%
!!! GGAGTTTTTTGAGTAATCAT+CGG + Chr1:68113664-68113683 None:intergenic 30.0%
AAGCTGACAAAATCAAAGGA+TGG - Chr1:68113769-68113788 MsG0180003779.01.T01:CDS 35.0%
CAAATGTTGACCATTGAATG+AGG + Chr1:68113620-68113639 None:intergenic 35.0%
CCAGAAGAATCAAAAACTGA+GGG + Chr1:68113829-68113848 None:intergenic 35.0%
GCCACTTCTTTAATTATCCA+TGG - Chr1:68113577-68113596 MsG0180003779.01.T01:CDS 35.0%
TCCAGAAGAATCAAAAACTG+AGG + Chr1:68113830-68113849 None:intergenic 35.0%
TGTGAAGCTGACAAAATCAA+AGG - Chr1:68113765-68113784 MsG0180003779.01.T01:CDS 35.0%
! TTTGATTCTTCTGGAGAAGT+AGG - Chr1:68113835-68113854 MsG0180003779.01.T01:CDS 35.0%
AAAGGATGGAACAAATGGCA+TGG - Chr1:68113783-68113802 MsG0180003779.01.T01:CDS 40.0%
AGTATATAATGCGGACTCCA+CGG - Chr1:68113959-68113978 MsG0180003779.01.T01:CDS 40.0%
ATACTTGGCTTCAATTCCTG+AGG + Chr1:68113944-68113963 None:intergenic 40.0%
ATCATCGGTAACCTCAAACT+TGG + Chr1:68113649-68113668 None:intergenic 40.0%
ATTTGCATGTGCCAAGTTTG+AGG - Chr1:68113635-68113654 MsG0180003779.01.T01:CDS 40.0%
GGATAGGTTCATGAGATTCA+TGG - Chr1:68113987-68114006 MsG0180003779.01.T01:CDS 40.0%
TACTTGGCTTCAATTCCTGA+GGG + Chr1:68113943-68113962 None:intergenic 40.0%
TGGAGTCCGCATTATATACT+TGG + Chr1:68113959-68113978 None:intergenic 40.0%
! GATTTTGTCAGCTTCACATC+AGG + Chr1:68113763-68113782 None:intergenic 40.0%
!! CCCTCAGTTTTTGATTCTTC+TGG - Chr1:68113826-68113845 MsG0180003779.01.T01:CDS 40.0%
AAAAACTCCCATGCACATGC+CGG - Chr1:68113675-68113694 MsG0180003779.01.T01:CDS 45.0%
ATGATAACCGGCATGTGCAT+GGG + Chr1:68113685-68113704 None:intergenic 45.0%
CAAATGGCATGGCTATCTTC+AGG - Chr1:68113794-68113813 MsG0180003779.01.T01:CDS 45.0%
GAGCGATACTCTGATGCTAA+TGG - Chr1:68114009-68114028 MsG0180003779.01.T01:CDS 45.0%
GTATATAATGCGGACTCCAC+GGG - Chr1:68113960-68113979 MsG0180003779.01.T01:CDS 45.0%
CATGATAACCGGCATGTGCA+TGG + Chr1:68113686-68113705 None:intergenic 50.0%
GGAGAAGTAGGCGACATTAC+TGG - Chr1:68113847-68113866 MsG0180003779.01.T01:CDS 50.0%
TTCCCCGCCATCATGATAAC+CGG + Chr1:68113697-68113716 None:intergenic 50.0%
TTTGTCAACGGAAAGCCCTC+AGG - Chr1:68113925-68113944 MsG0180003779.01.T01:CDS 50.0%
! ATGCCGGTTATCATGATGGC+GGG - Chr1:68113691-68113710 MsG0180003779.01.T01:CDS 50.0%
! CATGCCGGTTATCATGATGG+CGG - Chr1:68113690-68113709 MsG0180003779.01.T01:CDS 50.0%
! GCACATGCCGGTTATCATGA+TGG - Chr1:68113687-68113706 MsG0180003779.01.T01:CDS 50.0%
ATGAACCTATCCCAGTCCCG+TGG + Chr1:68113979-68113998 None:intergenic 55.0%
! TGCCGGTTATCATGATGGCG+GGG - Chr1:68113692-68113711 MsG0180003779.01.T01:CDS 55.0%
AATGCGGACTCCACGGGACT+GGG - Chr1:68113966-68113985 MsG0180003779.01.T01:CDS 60.0%
TAATGCGGACTCCACGGGAC+TGG - Chr1:68113965-68113984 MsG0180003779.01.T01:CDS 60.0%
GGACTCCACGGGACTGGGAT+AGG - Chr1:68113971-68113990 MsG0180003779.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 68113541 68114053 68113541 ID=MsG0180003779.01;Name=MsG0180003779.01
Chr1 mRNA 68113541 68114053 68113541 ID=MsG0180003779.01.T01;Parent=MsG0180003779.01;Name=MsG0180003779.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|170
Chr1 exon 68113541 68114053 68113541 ID=MsG0180003779.01.T01:exon:11015;Parent=MsG0180003779.01.T01
Chr1 CDS 68113541 68114053 68113541 ID=MsG0180003779.01.T01:cds;Parent=MsG0180003779.01.T01
Gene Sequence

>MsG0180003779.01.T01

ATGCATGATGAAAATTATAGACTATTTGTTTATGCAGCCACTTCTTTAATTATCCATGGAAGTTTGAACTTAAATTCCTCATTCAATGGTCAACATTTGCATGTGCCAAGTTTGAGGTTACCGATGATTACTCAAAAAACTCCCATGCACATGCCGGTTATCATGATGGCGGGGAAGCCCAAAATTCAATTCATTCAAGGAACTGATGAACTGACAATACCTGATGTGAAGCTGACAAAATCAAAGGATGGAACAAATGGCATGGCTATCTTCAGGTTTGATCAACCCTCAGTTTTTGATTCTTCTGGAGAAGTAGGCGACATTACTGGTTTTTATATGATTGATGAGGAAGGAGTCCTTCAATCTGTTGATGTAAATGCTAAATTTGTCAACGGAAAGCCCTCAGGAATTGAAGCCAAGTATATAATGCGGACTCCACGGGACTGGGATAGGTTCATGAGATTCATGGAGCGATACTCTGATGCTAATGGTTTGCAATTCATAAAATATTGA

Protein sequence

>MsG0180003779.01.T01

MHDENYRLFVYAATSLIIHGSLNLNSSFNGQHLHVPSLRLPMITQKTPMHMPVIMMAGKPKIQFIQGTDELTIPDVKLTKSKDGTNGMAIFRFDQPSVFDSSGEVGDITGFYMIDEEGVLQSVDVNAKFVNGKPSGIEAKYIMRTPRDWDRFMRFMERYSDANGLQFIKY*