Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | RHN82474.1 | 95.4 | 587 | 20 | 1 | 1 | 587 | 601 | 1180 | 0 | 1129 |
| MsG0180005997.01.T01 | RHN82474.1 | 89.492 | 590 | 53 | 2 | 2 | 589 | 1195 | 1777 | 0 | 1082 |
| MsG0180005997.01.T01 | RHN82474.1 | 88.983 | 590 | 56 | 2 | 2 | 589 | 6 | 588 | 0 | 1072 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | F4I9E1 | 24.382 | 566 | 389 | 13 | 22 | 579 | 43 | 577 | 4.81E-26 | 116 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | A0A396JZ33 | 95.400 | 587 | 20 | 1 | 1 | 587 | 601 | 1180 | 0.0 | 1129 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0180005997.01 | 0.814846 | 1.251335e-51 | 8.489231e-49 |
| MsG0180001050.01 | MsG0180005997.01 | 0.838649 | 2.537076e-57 | 3.420297e-54 |
| MsG0180005996.01 | MsG0180005997.01 | 0.842081 | 3.215300e-58 | 4.820581e-55 |
| MsG0180005997.01 | MsG0280011053.01 | 0.841804 | 3.805112e-58 | 5.654832e-55 |
| MsG0180005997.01 | MsG0280011152.01 | 0.819262 | 1.273933e-52 | 9.755199e-50 |
| MsG0180005997.01 | MsG0280011350.01 | 0.805532 | 1.275920e-49 | 6.766366e-47 |
| MsG0180005997.01 | MsG0380011613.01 | 0.824724 | 6.910596e-54 | 6.172597e-51 |
| MsG0180005997.01 | MsG0380016644.01 | 0.839591 | 1.446282e-57 | 2.006601e-54 |
| MsG0180005997.01 | MsG0480018904.01 | 0.802384 | 5.756444e-49 | 2.816075e-46 |
| MsG0180005997.01 | MsG0480019106.01 | 0.802740 | 4.860903e-49 | 2.399553e-46 |
| MsG0180005997.01 | MsG0480022125.01 | 0.813083 | 3.062115e-51 | 1.980376e-48 |
| MsG0180005997.01 | MsG0480022507.01 | 0.801164 | 1.024586e-48 | 4.859434e-46 |
| MsG0180005997.01 | MsG0480022508.01 | 0.825999 | 3.451167e-54 | 3.196464e-51 |
| MsG0180005997.01 | MsG0480022680.01 | 0.801737 | 7.819915e-49 | 3.762641e-46 |
| MsG0180005997.01 | MsG0480022736.01 | 0.827737 | 1.325831e-54 | 1.291093e-51 |
| MsG0180005997.01 | MsG0580025180.01 | 0.821855 | 3.233802e-53 | 2.662195e-50 |
| MsG0180005997.01 | MsG0580028758.01 | 0.800733 | 1.254666e-48 | 5.885930e-46 |
| MsG0180005997.01 | MsG0580029930.01 | 0.817920 | 2.566727e-52 | 1.894169e-49 |
| MsG0180005997.01 | MsG0680031429.01 | 0.814848 | 1.250121e-51 | 8.481480e-49 |
| MsG0180005997.01 | MsG0780039968.01 | 0.813836 | 2.091773e-51 | 1.380822e-48 |
| MsG0180005997.01 | MsG0780040060.01 | 0.812564 | 3.978866e-51 | 2.538281e-48 |
| MsG0180005997.01 | MsG0780040480.01 | 0.815022 | 1.143680e-51 | 7.795928e-49 |
| MsG0180005997.01 | MsG0780041589.01 | 0.810285 | 1.243425e-50 | 7.464759e-48 |
| MsG0180005997.01 | MsG0880043185.01 | 0.818214 | 2.203345e-52 | 1.639055e-49 |
| MsG0180005997.01 | MsG0880043824.01 | 0.822502 | 2.289239e-53 | 1.919627e-50 |
| MsG0180005997.01 | MsG0880044669.01 | 0.830845 | 2.334180e-55 | 2.488942e-52 |
| MsG0180005997.01 | MsG0880045861.01 | 0.820301 | 7.372496e-53 | 5.811333e-50 |
| MsG0180005997.01 | MsG0880047081.01 | 0.805988 | 1.022998e-49 | 5.489462e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | MTR_1g111530 | 94.719 | 587 | 26 | 1 | 1 | 587 | 1 | 582 | 0.0 | 1104 |
| MsG0180005997.01.T01 | MTR_1g111540 | 89.456 | 588 | 57 | 1 | 2 | 589 | 6 | 588 | 0.0 | 1065 |
| MsG0180005997.01.T01 | MTR_1g111510 | 89.116 | 588 | 59 | 1 | 2 | 589 | 6 | 588 | 0.0 | 1055 |
| MsG0180005997.01.T01 | MTR_1g111550 | 71.601 | 581 | 151 | 3 | 12 | 584 | 15 | 589 | 0.0 | 853 |
| MsG0180005997.01.T01 | MTR_7g102880 | 60.302 | 597 | 213 | 6 | 5 | 584 | 12 | 601 | 0.0 | 710 |
| MsG0180005997.01.T01 | MTR_1g050550 | 58.065 | 589 | 237 | 4 | 6 | 584 | 8 | 596 | 0.0 | 699 |
| MsG0180005997.01.T01 | MTR_1g059080 | 57.168 | 572 | 232 | 4 | 6 | 573 | 8 | 570 | 0.0 | 669 |
| MsG0180005997.01.T01 | MTR_2g100400 | 34.261 | 575 | 312 | 8 | 19 | 567 | 4 | 538 | 1.61e-106 | 331 |
| MsG0180005997.01.T01 | MTR_5g090570 | 34.054 | 555 | 325 | 8 | 22 | 567 | 11 | 533 | 2.15e-103 | 323 |
| MsG0180005997.01.T01 | MTR_7g029015 | 34.783 | 575 | 323 | 11 | 22 | 557 | 15 | 576 | 2.87e-101 | 319 |
| MsG0180005997.01.T01 | MTR_3g099150 | 34.043 | 564 | 321 | 11 | 22 | 558 | 15 | 554 | 2.12e-95 | 303 |
| MsG0180005997.01.T01 | MTR_4g073690 | 33.455 | 547 | 327 | 9 | 22 | 558 | 15 | 534 | 1.40e-90 | 290 |
| MsG0180005997.01.T01 | MTR_4g116210 | 32.374 | 556 | 348 | 9 | 23 | 558 | 12 | 559 | 1.23e-85 | 278 |
| MsG0180005997.01.T01 | MTR_5g022380 | 31.907 | 561 | 332 | 9 | 22 | 573 | 13 | 532 | 2.33e-81 | 265 |
| MsG0180005997.01.T01 | MTR_2g450710 | 30.466 | 558 | 349 | 8 | 23 | 558 | 13 | 553 | 3.26e-79 | 261 |
| MsG0180005997.01.T01 | MTR_5g022380 | 31.965 | 463 | 265 | 9 | 120 | 573 | 1 | 422 | 1.54e-61 | 210 |
| MsG0180005997.01.T01 | MTR_4g073690 | 32.997 | 397 | 238 | 8 | 22 | 408 | 15 | 393 | 2.73e-57 | 197 |
| MsG0180005997.01.T01 | MTR_2g104250 | 31.878 | 229 | 113 | 5 | 330 | 558 | 104 | 289 | 7.29e-31 | 123 |
| MsG0180005997.01.T01 | MTR_7g083660 | 27.210 | 577 | 372 | 16 | 22 | 571 | 7 | 562 | 3.46e-30 | 125 |
| MsG0180005997.01.T01 | MTR_5g026350 | 25.043 | 579 | 388 | 15 | 22 | 570 | 7 | 569 | 2.63e-27 | 116 |
| MsG0180005997.01.T01 | MTR_5g095240 | 66.197 | 71 | 24 | 0 | 494 | 564 | 2 | 72 | 4.39e-26 | 102 |
| MsG0180005997.01.T01 | MTR_3g466040 | 24.731 | 558 | 365 | 12 | 11 | 558 | 1 | 513 | 1.16e-23 | 105 |
| MsG0180005997.01.T01 | MTR_3g466110 | 22.374 | 514 | 356 | 7 | 20 | 527 | 5 | 481 | 3.30e-19 | 91.3 |
| MsG0180005997.01.T01 | MTR_8g071920 | 22.483 | 596 | 404 | 16 | 13 | 572 | 39 | 612 | 2.58e-18 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | AT2G39210 | 71.658 | 561 | 156 | 2 | 9 | 568 | 8 | 566 | 0.0 | 841 |
| MsG0180005997.01.T01 | AT2G28120 | 61.441 | 555 | 208 | 1 | 16 | 570 | 11 | 559 | 0.0 | 699 |
| MsG0180005997.01.T01 | AT1G74780 | 36.219 | 566 | 303 | 7 | 22 | 568 | 7 | 533 | 3.43e-114 | 351 |
| MsG0180005997.01.T01 | AT1G18940 | 36.071 | 560 | 299 | 8 | 14 | 558 | 1 | 516 | 8.97e-110 | 339 |
| MsG0180005997.01.T01 | AT2G34355 | 34.271 | 569 | 305 | 8 | 16 | 564 | 1 | 520 | 2.59e-103 | 322 |
| MsG0180005997.01.T01 | AT2G34350 | 34.107 | 560 | 308 | 8 | 22 | 564 | 7 | 522 | 3.26e-98 | 309 |
| MsG0180005997.01.T01 | AT2G34350 | 34.107 | 560 | 308 | 8 | 22 | 564 | 7 | 522 | 3.26e-98 | 309 |
| MsG0180005997.01.T01 | AT5G50520 | 33.455 | 547 | 327 | 7 | 19 | 557 | 13 | 530 | 1.05e-96 | 306 |
| MsG0180005997.01.T01 | AT5G50630 | 33.455 | 547 | 327 | 7 | 19 | 557 | 13 | 530 | 1.05e-96 | 306 |
| MsG0180005997.01.T01 | AT5G14120 | 33.507 | 576 | 351 | 9 | 16 | 566 | 10 | 578 | 1.73e-96 | 306 |
| MsG0180005997.01.T01 | AT4G34950 | 32.545 | 550 | 342 | 6 | 22 | 557 | 16 | 550 | 2.45e-93 | 298 |
| MsG0180005997.01.T01 | AT3G01930 | 31.911 | 586 | 348 | 8 | 19 | 567 | 13 | 584 | 1.15e-92 | 296 |
| MsG0180005997.01.T01 | AT2G16660 | 34.011 | 541 | 327 | 9 | 22 | 557 | 14 | 529 | 3.51e-90 | 289 |
| MsG0180005997.01.T01 | AT1G80530 | 30.866 | 554 | 342 | 10 | 23 | 558 | 14 | 544 | 8.26e-73 | 243 |
| MsG0180005997.01.T01 | AT2G34355 | 33.014 | 418 | 219 | 7 | 159 | 564 | 2 | 370 | 1.79e-65 | 219 |
| MsG0180005997.01.T01 | AT3G01930 | 30.335 | 478 | 296 | 8 | 120 | 567 | 1 | 471 | 4.24e-65 | 221 |
| MsG0180005997.01.T01 | AT3G01930 | 31.604 | 424 | 242 | 6 | 19 | 405 | 13 | 425 | 1.27e-57 | 199 |
| MsG0180005997.01.T01 | AT2G16660 | 33.418 | 392 | 240 | 8 | 22 | 408 | 14 | 389 | 1.53e-55 | 193 |
| MsG0180005997.01.T01 | AT4G19450 | 25.310 | 565 | 381 | 14 | 22 | 558 | 7 | 558 | 5.27e-31 | 128 |
| MsG0180005997.01.T01 | AT5G45275 | 25.606 | 578 | 376 | 16 | 22 | 568 | 7 | 561 | 1.64e-30 | 126 |
| MsG0180005997.01.T01 | AT3G01630 | 25.090 | 558 | 361 | 15 | 22 | 558 | 41 | 562 | 5.20e-30 | 125 |
| MsG0180005997.01.T01 | AT3G01630 | 25.090 | 558 | 361 | 15 | 22 | 558 | 23 | 544 | 6.02e-30 | 124 |
| MsG0180005997.01.T01 | AT1G31470 | 24.382 | 566 | 389 | 13 | 22 | 579 | 43 | 577 | 4.90e-27 | 116 |
| MsG0180005997.01.T01 | AT4G19450 | 25.799 | 407 | 269 | 10 | 22 | 401 | 7 | 407 | 9.22e-23 | 101 |
Find 99 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCAACTTTGTATAATTT+TGG | 0.150653 | 1:+98617057 | MsG0180005997.01.T01:CDS |
| AACATATCTTCCCACCATTT+TGG | 0.169832 | 1:-98616601 | None:intergenic |
| CGAATCTCGCGGAGCTGTTT+TGG | 0.174120 | 1:+98616084 | MsG0180005997.01.T01:CDS |
| GTAAGTATTTGGAATTATTT+AGG | 0.199881 | 1:+98616808 | MsG0180005997.01.T01:CDS |
| TGTTGTTGTTAACCTTATTT+TGG | 0.234354 | 1:+98617269 | MsG0180005997.01.T01:CDS |
| TACATCTCACTTGCTCTTGC+TGG | 0.247387 | 1:+98616307 | MsG0180005997.01.T01:CDS |
| ATTGCAGCTGCAACTTTCTT+TGG | 0.249523 | 1:+98617246 | MsG0180005997.01.T01:CDS |
| TTGTGTCTTTAGTAAGTATT+TGG | 0.250564 | 1:+98616797 | MsG0180005997.01.T01:CDS |
| GGTGCTATACTTAATTTCTT+TGG | 0.261307 | 1:+98615920 | MsG0180005997.01.T01:CDS |
| TCTTTGGTTACTTCATGATT+TGG | 0.283109 | 1:+98615936 | MsG0180005997.01.T01:CDS |
| TTGTCCTAGCCACCAAAATA+AGG | 0.303085 | 1:-98617281 | None:intergenic |
| GTGGCATGTATTTGTGGTAT+TGG | 0.316784 | 1:+98616697 | MsG0180005997.01.T01:CDS |
| GTGACATATACAAGAGATAT+AGG | 0.328395 | 1:+98617319 | MsG0180005997.01.T01:CDS |
| TGCTCTTGCTGGTTTCTTGT+TGG | 0.334336 | 1:+98616318 | MsG0180005997.01.T01:CDS |
| GGAATTCTAAAAGGATATCT+TGG | 0.343048 | 1:+98616106 | MsG0180005997.01.T01:CDS |
| TATGCAGCTTCAGTGATTAT+CGG | 0.345986 | 1:+98616967 | MsG0180005997.01.T01:CDS |
| TCGACAAGTCAATGAGCTTA+AGG | 0.355352 | 1:+98616267 | MsG0180005997.01.T01:CDS |
| CTTGGAAAGCATAGAATAGA+AGG | 0.363836 | 1:+98617171 | MsG0180005997.01.T01:CDS |
| AGTTTCACACAGGGTGAGTT+TGG | 0.364101 | 1:+98616367 | MsG0180005997.01.T01:CDS |
| CAAAACAGCTCCGCGAGATT+CGG | 0.367405 | 1:-98616083 | None:intergenic |
| AAAACAGCTCCGCGAGATTC+GGG | 0.368427 | 1:-98616082 | None:intergenic |
| AGCAATGTTGGTGTCCTTGC+AGG | 0.392854 | 1:+98615854 | MsG0180005997.01.T01:CDS |
| TTGACAGCAGTTGATAATCT+TGG | 0.396343 | 1:+98616727 | MsG0180005997.01.T01:CDS |
| TGGCAATTGGTGTTCATGAT+TGG | 0.409001 | 1:+98615645 | MsG0180005997.01.T01:CDS |
| AGCTCATTGACTTGTCGAAC+AGG | 0.413360 | 1:-98616262 | None:intergenic |
| GGCAATTGGTGTTCATGATT+GGG | 0.421592 | 1:+98615646 | MsG0180005997.01.T01:CDS |
| ATATAGGGAAGAAGCTGAAA+TGG | 0.422458 | 1:+98617335 | MsG0180005997.01.T01:CDS |
| CTATTTGTGGCATGTATTTG+TGG | 0.422626 | 1:+98616691 | MsG0180005997.01.T01:CDS |
| ACCACCGTCCGGTGATGGCT+TGG | 0.430632 | 1:-98615691 | None:intergenic |
| AGACATGTTGATACTATTTG+TGG | 0.431839 | 1:+98616678 | MsG0180005997.01.T01:CDS |
| GTATAATCATCACCTCTTGC+CGG | 0.432509 | 1:-98616631 | None:intergenic |
| CAAAGAAGCTAAGAAACAAT+TGG | 0.433049 | 1:+98617143 | MsG0180005997.01.T01:CDS |
| GGAAGTATAACAAGTCCAAT+TGG | 0.435702 | 1:+98617078 | MsG0180005997.01.T01:CDS |
| ATCCAAGTAAATATGGCAAT+TGG | 0.438218 | 1:+98615632 | None:intergenic |
| AAAGGATATCTTGGTCTTAG+TGG | 0.440413 | 1:+98616115 | MsG0180005997.01.T01:CDS |
| GCCGGAGCCACCTACATGTT+CGG | 0.441437 | 1:+98615749 | MsG0180005997.01.T01:CDS |
| AAACTGCAAGACCACATGTT+TGG | 0.447304 | 1:+98615975 | MsG0180005997.01.T01:CDS |
| TGTTGAACTGAGGTTGTTGT+TGG | 0.458257 | 1:-98616542 | None:intergenic |
| TTATTGAAGAGAACAAGATT+TGG | 0.460711 | 1:+98616443 | MsG0180005997.01.T01:CDS |
| TTAAGCACATATAAGCCAAT+TGG | 0.462132 | 1:-98617093 | None:intergenic |
| GTTGATAATCTTGGTCAGAT+AGG | 0.465696 | 1:+98616736 | MsG0180005997.01.T01:CDS |
| AGAGTCAAAGAAAGCATAAG+AGG | 0.471726 | 1:-98616889 | None:intergenic |
| GAAGCTGCATAGAGACCCTT+TGG | 0.475496 | 1:-98616955 | None:intergenic |
| CCTTCTGTTACTATTTGCAC+TGG | 0.477008 | 1:-98616499 | None:intergenic |
| TGACATATACAAGAGATATA+GGG | 0.477639 | 1:+98617320 | MsG0180005997.01.T01:CDS |
| ACCGAACATGTAGGTGGCTC+CGG | 0.477927 | 1:-98615750 | None:intergenic |
| CAACGAAATCTCTCCACCTT+GGG | 0.480872 | 1:+98615883 | MsG0180005997.01.T01:CDS |
| GACTCTCTTTGTCTCGTGTG+TGG | 0.483546 | 1:+98616906 | MsG0180005997.01.T01:CDS |
| AACACAAGTAACAAGTGAAC+CGG | 0.486587 | 1:-98616050 | None:intergenic |
| ACTCTCTTTGTCTCGTGTGT+GGG | 0.489234 | 1:+98616907 | MsG0180005997.01.T01:CDS |
| TAAGGAGGTCTAGTGTGCTT+TGG | 0.491975 | 1:-98615811 | None:intergenic |
| AAAATGTTCTGAAACAAATC+CGG | 0.495163 | 1:-98616842 | None:intergenic |
| GATAGCATTTGGTGTGCCAA+AGG | 0.517982 | 1:+98616939 | MsG0180005997.01.T01:CDS |
| ATGTGTCTTTACATTTGCAT+AGG | 0.521059 | 1:+98616001 | MsG0180005997.01.T01:CDS |
| GCATGTATTTGTGGTATTGG+TGG | 0.523323 | 1:+98616700 | MsG0180005997.01.T01:CDS |
| GCACACCAAATGCTATCAAA+AGG | 0.525284 | 1:-98616933 | None:intergenic |
| GACAAACCACCGTCCGGTGA+TGG | 0.525568 | 1:-98615696 | None:intergenic |
| ACAGAAGAAGATGACAAAGT+TGG | 0.527636 | 1:+98617381 | MsG0180005997.01.T01:CDS |
| GGCAGCTGACATGATTAGAA+AGG | 0.528910 | 1:-98615729 | None:intergenic |
| ATAGCATTTGGTGTGCCAAA+GGG | 0.533343 | 1:+98616940 | MsG0180005997.01.T01:CDS |
| TTTCTAATCATGTCAGCTGC+CGG | 0.534042 | 1:+98615731 | MsG0180005997.01.T01:CDS |
| AAGAAACAATTGGAAGCACT+TGG | 0.542138 | 1:+98617153 | MsG0180005997.01.T01:CDS |
| TAGGGAAGAAGCTGAAATGG+TGG | 0.542633 | 1:+98617338 | MsG0180005997.01.T01:CDS |
| AAGAATTTCCCCGAATCTCG+CGG | 0.547653 | 1:+98616073 | MsG0180005997.01.T01:CDS |
| TATTTATTATGATGACTCAA+AGG | 0.547885 | 1:+98616165 | MsG0180005997.01.T01:CDS |
| CCGTCCGGTGATGGCTTGGA+CGG | 0.551701 | 1:-98615687 | None:intergenic |
| AAAGCATAGAATAGAAGGGC+AGG | 0.553260 | 1:+98617176 | MsG0180005997.01.T01:CDS |
| TCAACGAAATCTCTCCACCT+TGG | 0.557251 | 1:+98615882 | MsG0180005997.01.T01:CDS |
| GGAAGATATGTTCAATCCAC+CGG | 0.560480 | 1:+98616612 | MsG0180005997.01.T01:CDS |
| GTTTGGTTTAAGTGCTGCTG+TGG | 0.571063 | 1:+98616384 | MsG0180005997.01.T01:CDS |
| GTGCTTAATGTGAAGATCAC+TGG | 0.572972 | 1:+98617108 | MsG0180005997.01.T01:CDS |
| AAAGCGAATGAAATAGCAGC+AGG | 0.578973 | 1:-98616214 | None:intergenic |
| GGGCAGGAATTGAATTGTGT+TGG | 0.581328 | 1:+98617192 | MsG0180005997.01.T01:CDS |
| CTCACCGTCCAAGCCATCAC+CGG | 0.581421 | 1:+98615683 | MsG0180005997.01.T01:CDS |
| AAAGAAAGCATAAGAGGACG+AGG | 0.591288 | 1:-98616883 | None:intergenic |
| GAAGAGTAGTAGTTTCACAC+AGG | 0.592988 | 1:+98616357 | MsG0180005997.01.T01:CDS |
| GGAAGAAGCTGAAATGGTGG+AGG | 0.594474 | 1:+98617341 | MsG0180005997.01.T01:CDS |
| TTTCGTTGATTAGTCCTGCA+AGG | 0.596687 | 1:-98615868 | None:intergenic |
| AACAGGTTTCATATACCGAA+TGG | 0.597164 | 1:-98616245 | None:intergenic |
| ATGTTCAATCCACCGGCAAG+AGG | 0.616234 | 1:+98616619 | MsG0180005997.01.T01:CDS |
| TAATCATCACCTCTTGCCGG+TGG | 0.623378 | 1:-98616628 | None:intergenic |
| AGCGACATAAAAGAAACACT+TGG | 0.623492 | 1:+98615782 | MsG0180005997.01.T01:CDS |
| GGAGTATAGACCGAACATGT+AGG | 0.626603 | 1:-98615759 | None:intergenic |
| GCAATTGGTGTTCATGATTG+GGG | 0.629985 | 1:+98615647 | MsG0180005997.01.T01:CDS |
| AAACAGCTCCGCGAGATTCG+GGG | 0.632787 | 1:-98616081 | None:intergenic |
| CCAGTGCAAATAGTAACAGA+AGG | 0.634763 | 1:+98616499 | MsG0180005997.01.T01:CDS |
| ATGGTGGAGGTAAAGAACGA+GGG | 0.640025 | 1:+98617354 | MsG0180005997.01.T01:CDS |
| CAGCAGAGGTTGTTGAACTG+AGG | 0.642991 | 1:-98616552 | None:intergenic |
| TTGGAAAGCATAGAATAGAA+GGG | 0.652646 | 1:+98617172 | MsG0180005997.01.T01:CDS |
| CCTATTGCTAAAACTACCCA+AGG | 0.655732 | 1:-98615899 | None:intergenic |
| CCGTCCAAGCCATCACCGGA+CGG | 0.660584 | 1:+98615687 | MsG0180005997.01.T01:CDS |
| AGACACATTTGCCAAACATG+TGG | 0.663012 | 1:-98615986 | None:intergenic |
| AAGAGTAGTAGTTTCACACA+GGG | 0.669994 | 1:+98616358 | MsG0180005997.01.T01:CDS |
| GACTGAAACTGAAACAGCAG+AGG | 0.678973 | 1:-98616566 | None:intergenic |
| TCCAAGCCATCACCGGACGG+TGG | 0.681683 | 1:+98615690 | MsG0180005997.01.T01:CDS |
| GTATAGACCGAACATGTAGG+TGG | 0.686930 | 1:-98615756 | None:intergenic |
| ATTGCTAAAACTACCCAAGG+TGG | 0.696114 | 1:-98615896 | None:intergenic |
| AAGATGACAAAGTTGGCATG+CGG | 0.700634 | 1:+98617388 | MsG0180005997.01.T01:CDS |
| AATGGTGGAGGTAAAGAACG+AGG | 0.714318 | 1:+98617353 | MsG0180005997.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTATTATGATGACTCAA+AGG | + | Chr1:98616165-98616184 | MsG0180005997.01.T01:CDS | 20.0% |
| !!! | CAAAATCTTTGAAAAAACTA+AGG | - | Chr1:98615832-98615851 | None:intergenic | 20.0% |
| !!! | CTTAGTTTTTTCAAAGATTT+TGG | + | Chr1:98615830-98615849 | MsG0180005997.01.T01:CDS | 20.0% |
| !!! | GTAAGTATTTGGAATTATTT+AGG | + | Chr1:98616808-98616827 | MsG0180005997.01.T01:CDS | 20.0% |
| !!! | TATGCAACTTTGTATAATTT+TGG | + | Chr1:98617057-98617076 | MsG0180005997.01.T01:CDS | 20.0% |
| ! | AAAAAAAGATCCAAAATGGT+GGG | + | Chr1:98616591-98616610 | MsG0180005997.01.T01:CDS | 25.0% |
| ! | GATATAATGGCAAAAAGTAA+TGG | - | Chr1:98617015-98617034 | None:intergenic | 25.0% |
| ! | TGACATATACAAGAGATATA+GGG | + | Chr1:98617320-98617339 | MsG0180005997.01.T01:CDS | 25.0% |
| ! | TTATTGAAGAGAACAAGATT+TGG | + | Chr1:98616443-98616462 | MsG0180005997.01.T01:CDS | 25.0% |
| ! | TTGTGTCTTTAGTAAGTATT+TGG | + | Chr1:98616797-98616816 | MsG0180005997.01.T01:CDS | 25.0% |
| !! | AATCTTTGAAAAAACTAAGG+AGG | - | Chr1:98615829-98615848 | None:intergenic | 25.0% |
| !! | ACCATTTTGGATCTTTTTTT+GGG | - | Chr1:98616591-98616610 | None:intergenic | 25.0% |
| !! | TCCAAAAAGTTCTGATATAA+TGG | - | Chr1:98617028-98617047 | None:intergenic | 25.0% |
| !!! | AAAATGTTCTGAAACAAATC+CGG | - | Chr1:98616845-98616864 | None:intergenic | 25.0% |
| !!! | TCTTGCAGTTTTTTTTGTTA+TGG | - | Chr1:98615966-98615985 | None:intergenic | 25.0% |
| !!! | TGTTGTTGTTAACCTTATTT+TGG | + | Chr1:98617269-98617288 | MsG0180005997.01.T01:CDS | 25.0% |
| AGACATGTTGATACTATTTG+TGG | + | Chr1:98616678-98616697 | MsG0180005997.01.T01:CDS | 30.0% | |
| ATGTGTCTTTACATTTGCAT+AGG | + | Chr1:98616001-98616020 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAAAAAAAGATCCAAAATGG+TGG | + | Chr1:98616590-98616609 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAAAGAAGCTAAGAAACAAT+TGG | + | Chr1:98617143-98617162 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAATAAAAACAACAGCAAGT+GGG | - | Chr1:98616429-98616448 | None:intergenic | 30.0% | |
| GTGACATATACAAGAGATAT+AGG | + | Chr1:98617319-98617338 | MsG0180005997.01.T01:CDS | 30.0% | |
| TCAATAAAAACAACAGCAAG+TGG | - | Chr1:98616430-98616449 | None:intergenic | 30.0% | |
| TCCCAAAAAAAGATCCAAAA+TGG | + | Chr1:98616587-98616606 | MsG0180005997.01.T01:CDS | 30.0% | |
| TCTTTGGTTACTTCATGATT+TGG | + | Chr1:98615936-98615955 | MsG0180005997.01.T01:CDS | 30.0% | |
| TTAAGCACATATAAGCCAAT+TGG | - | Chr1:98617096-98617115 | None:intergenic | 30.0% | |
| ! | AGGACAAGAAAGTTTTACAA+AGG | + | Chr1:98617297-98617316 | MsG0180005997.01.T01:CDS | 30.0% |
| ! | CACCATTTTGGATCTTTTTT+TGG | - | Chr1:98616592-98616611 | None:intergenic | 30.0% |
| ! | GCCATTATATCAGAACTTTT+TGG | + | Chr1:98617024-98617043 | MsG0180005997.01.T01:CDS | 30.0% |
| ! | GGAATTCTAAAAGGATATCT+TGG | + | Chr1:98616106-98616125 | MsG0180005997.01.T01:CDS | 30.0% |
| ! | GGTGCTATACTTAATTTCTT+TGG | + | Chr1:98615920-98615939 | MsG0180005997.01.T01:CDS | 30.0% |
| ! | TTGGAAAGCATAGAATAGAA+GGG | + | Chr1:98617172-98617191 | MsG0180005997.01.T01:CDS | 30.0% |
| !! | AAAGATTTTGGTAGCAATGT+TGG | + | Chr1:98615842-98615861 | MsG0180005997.01.T01:CDS | 30.0% |
| !! | AAAGTGCTTATGCTTTTCTT+TGG | - | Chr1:98616778-98616797 | None:intergenic | 30.0% |
| !! | AAGGCTTTGATTTTGCTTAT+AGG | + | Chr1:98616184-98616203 | MsG0180005997.01.T01:CDS | 30.0% |
| !! | CTTTGATTTTGCTTATAGGT+TGG | + | Chr1:98616188-98616207 | MsG0180005997.01.T01:CDS | 30.0% |
| !! | GTGATTATCGGATTTTGTTT+TGG | + | Chr1:98616979-98616998 | MsG0180005997.01.T01:CDS | 30.0% |
| !!! | TGTTGTTAACCTTATTTTGG+TGG | + | Chr1:98617272-98617291 | MsG0180005997.01.T01:CDS | 30.0% |
| AACACAAGTAACAAGTGAAC+CGG | - | Chr1:98616053-98616072 | None:intergenic | 35.0% | |
| AACAGGTTTCATATACCGAA+TGG | - | Chr1:98616248-98616267 | None:intergenic | 35.0% | |
| AAGAGTAGTAGTTTCACACA+GGG | + | Chr1:98616358-98616377 | MsG0180005997.01.T01:CDS | 35.0% | |
| ACAGAAGAAGATGACAAAGT+TGG | + | Chr1:98617381-98617400 | MsG0180005997.01.T01:CDS | 35.0% | |
| AGCGACATAAAAGAAACACT+TGG | + | Chr1:98615782-98615801 | MsG0180005997.01.T01:CDS | 35.0% | |
| ATATAGGGAAGAAGCTGAAA+TGG | + | Chr1:98617335-98617354 | MsG0180005997.01.T01:CDS | 35.0% | |
| CTATTTGTGGCATGTATTTG+TGG | + | Chr1:98616691-98616710 | MsG0180005997.01.T01:CDS | 35.0% | |
| TATGCAGCTTCAGTGATTAT+CGG | + | Chr1:98616967-98616986 | MsG0180005997.01.T01:CDS | 35.0% | |
| ! | AACATATCTTCCCACCATTT+TGG | - | Chr1:98616604-98616623 | None:intergenic | 35.0% |
| ! | AAGTGTTTCTTTTATGTCGC+TGG | - | Chr1:98615783-98615802 | None:intergenic | 35.0% |
| ! | AGAGTCAAAGAAAGCATAAG+AGG | - | Chr1:98616892-98616911 | None:intergenic | 35.0% |
| ! | CTTGGAAAGCATAGAATAGA+AGG | + | Chr1:98617171-98617190 | MsG0180005997.01.T01:CDS | 35.0% |
| ! | GAAGAGTAAAAAGCTTGCTT+TGG | + | Chr1:98616465-98616484 | MsG0180005997.01.T01:CDS | 35.0% |
| ! | GGAAGTATAACAAGTCCAAT+TGG | + | Chr1:98617078-98617097 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | AAAGGATATCTTGGTCTTAG+TGG | + | Chr1:98616115-98616134 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | AAGAAACAATTGGAAGCACT+TGG | + | Chr1:98617153-98617172 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | GTTGATAATCTTGGTCAGAT+AGG | + | Chr1:98616736-98616755 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | TATTTAGGACGTGTTTTCTC+CGG | + | Chr1:98616823-98616842 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | TCGCTTTTCTTAGAACCATT+CGG | + | Chr1:98616230-98616249 | MsG0180005997.01.T01:CDS | 35.0% |
| !! | TTGACAGCAGTTGATAATCT+TGG | + | Chr1:98616727-98616746 | MsG0180005997.01.T01:CDS | 35.0% |
| !!! | GATTTTGTTTTGGTGCTCAA+TGG | + | Chr1:98616989-98617008 | MsG0180005997.01.T01:CDS | 35.0% |
| !!! | GCTGTTTTGGGAATTCTAAA+AGG | + | Chr1:98616097-98616116 | MsG0180005997.01.T01:CDS | 35.0% |
| !!! | TTAACCTTATTTTGGTGGCT+AGG | + | Chr1:98617277-98617296 | MsG0180005997.01.T01:CDS | 35.0% |
| AAAAATGACAAACCACCGTC+CGG | - | Chr1:98615705-98615724 | None:intergenic | 40.0% | |
| AAACTGCAAGACCACATGTT+TGG | + | Chr1:98615975-98615994 | MsG0180005997.01.T01:CDS | 40.0% | |
| AAAGCATAGAATAGAAGGGC+AGG | + | Chr1:98617176-98617195 | MsG0180005997.01.T01:CDS | 40.0% | |
| AAAGCGAATGAAATAGCAGC+AGG | - | Chr1:98616217-98616236 | None:intergenic | 40.0% | |
| AAGATGACAAAGTTGGCATG+CGG | + | Chr1:98617388-98617407 | MsG0180005997.01.T01:CDS | 40.0% | |
| AGACACATTTGCCAAACATG+TGG | - | Chr1:98615989-98616008 | None:intergenic | 40.0% | |
| ATTGCAGCTGCAACTTTCTT+TGG | + | Chr1:98617246-98617265 | MsG0180005997.01.T01:CDS | 40.0% | |
| ATTGCTAAAACTACCCAAGG+TGG | - | Chr1:98615899-98615918 | None:intergenic | 40.0% | |
| CCAGTGCAAATAGTAACAGA+AGG | + | Chr1:98616499-98616518 | MsG0180005997.01.T01:CDS | 40.0% | |
| CCTATTGCTAAAACTACCCA+AGG | - | Chr1:98615902-98615921 | None:intergenic | 40.0% | |
| CCTTCTGTTACTATTTGCAC+TGG | - | Chr1:98616502-98616521 | None:intergenic | 40.0% | |
| GAAGAGTAGTAGTTTCACAC+AGG | + | Chr1:98616357-98616376 | MsG0180005997.01.T01:CDS | 40.0% | |
| GCACACCAAATGCTATCAAA+AGG | - | Chr1:98616936-98616955 | None:intergenic | 40.0% | |
| GGAAGATATGTTCAATCCAC+CGG | + | Chr1:98616612-98616631 | MsG0180005997.01.T01:CDS | 40.0% | |
| GTATAATCATCACCTCTTGC+CGG | - | Chr1:98616634-98616653 | None:intergenic | 40.0% | |
| GTGCTTAATGTGAAGATCAC+TGG | + | Chr1:98617108-98617127 | MsG0180005997.01.T01:CDS | 40.0% | |
| GTGGCATGTATTTGTGGTAT+TGG | + | Chr1:98616697-98616716 | MsG0180005997.01.T01:CDS | 40.0% | |
| TCGACAAGTCAATGAGCTTA+AGG | + | Chr1:98616267-98616286 | MsG0180005997.01.T01:CDS | 40.0% | |
| TGTTGAACTGAGGTTGTTGT+TGG | - | Chr1:98616545-98616564 | None:intergenic | 40.0% | |
| TTGTCCTAGCCACCAAAATA+AGG | - | Chr1:98617284-98617303 | None:intergenic | 40.0% | |
| TTTCTAATCATGTCAGCTGC+CGG | + | Chr1:98615731-98615750 | MsG0180005997.01.T01:CDS | 40.0% | |
| ! | AAAGAAAGCATAAGAGGACG+AGG | - | Chr1:98616886-98616905 | None:intergenic | 40.0% |
| ! | GCAATTGGTGTTCATGATTG+GGG | + | Chr1:98615647-98615666 | MsG0180005997.01.T01:CDS | 40.0% |
| ! | GCATGTATTTGTGGTATTGG+TGG | + | Chr1:98616700-98616719 | MsG0180005997.01.T01:CDS | 40.0% |
| ! | GGCAATTGGTGTTCATGATT+GGG | + | Chr1:98615646-98615665 | MsG0180005997.01.T01:CDS | 40.0% |
| ! | TCTCAGTCTTTTGCAAACAC+CGG | + | Chr1:98616031-98616050 | MsG0180005997.01.T01:CDS | 40.0% |
| ! | TGGCAATTGGTGTTCATGAT+TGG | + | Chr1:98615645-98615664 | MsG0180005997.01.T01:CDS | 40.0% |
| ! | TTTCGTTGATTAGTCCTGCA+AGG | - | Chr1:98615871-98615890 | None:intergenic | 40.0% |
| !! | ATAGCATTTGGTGTGCCAAA+GGG | + | Chr1:98616940-98616959 | MsG0180005997.01.T01:CDS | 40.0% |
| !! | GGACACCTTTTGATAGCATT+TGG | + | Chr1:98616928-98616947 | MsG0180005997.01.T01:CDS | 40.0% |
| !!! | CCTTGGGTAGTTTTAGCAAT+AGG | + | Chr1:98615899-98615918 | MsG0180005997.01.T01:CDS | 40.0% |
| AAGAATTTCCCCGAATCTCG+CGG | + | Chr1:98616073-98616092 | MsG0180005997.01.T01:CDS | 45.0% | |
| ACTCTCTTTGTCTCGTGTGT+GGG | + | Chr1:98616907-98616926 | MsG0180005997.01.T01:CDS | 45.0% | |
| AGTTTCACACAGGGTGAGTT+TGG | + | Chr1:98616367-98616386 | MsG0180005997.01.T01:CDS | 45.0% | |
| CAACGAAATCTCTCCACCTT+GGG | + | Chr1:98615883-98615902 | MsG0180005997.01.T01:CDS | 45.0% | |
| GACTGAAACTGAAACAGCAG+AGG | - | Chr1:98616569-98616588 | None:intergenic | 45.0% | |
| GGAGTATAGACCGAACATGT+AGG | - | Chr1:98615762-98615781 | None:intergenic | 45.0% | |
| GGCAGCTGACATGATTAGAA+AGG | - | Chr1:98615732-98615751 | None:intergenic | 45.0% | |
| GGGCAGGAATTGAATTGTGT+TGG | + | Chr1:98617192-98617211 | MsG0180005997.01.T01:CDS | 45.0% | |
| GTATAGACCGAACATGTAGG+TGG | - | Chr1:98615759-98615778 | None:intergenic | 45.0% | |
| TAAGGAGGTCTAGTGTGCTT+TGG | - | Chr1:98615814-98615833 | None:intergenic | 45.0% | |
| TACATCTCACTTGCTCTTGC+TGG | + | Chr1:98616307-98616326 | MsG0180005997.01.T01:CDS | 45.0% | |
| TAGGGAAGAAGCTGAAATGG+TGG | + | Chr1:98617338-98617357 | MsG0180005997.01.T01:CDS | 45.0% | |
| TCAACGAAATCTCTCCACCT+TGG | + | Chr1:98615882-98615901 | MsG0180005997.01.T01:CDS | 45.0% | |
| ! | AATGGTGGAGGTAAAGAACG+AGG | + | Chr1:98617353-98617372 | MsG0180005997.01.T01:CDS | 45.0% |
| ! | ATGGTGGAGGTAAAGAACGA+GGG | + | Chr1:98617354-98617373 | MsG0180005997.01.T01:CDS | 45.0% |
| ! | GTTTGGTTTAAGTGCTGCTG+TGG | + | Chr1:98616384-98616403 | MsG0180005997.01.T01:CDS | 45.0% |
| !! | AGCTCATTGACTTGTCGAAC+AGG | - | Chr1:98616265-98616284 | None:intergenic | 45.0% |
| !! | GATAGCATTTGGTGTGCCAA+AGG | + | Chr1:98616939-98616958 | MsG0180005997.01.T01:CDS | 45.0% |
| !! | TGCTCTTGCTGGTTTCTTGT+TGG | + | Chr1:98616318-98616337 | MsG0180005997.01.T01:CDS | 45.0% |
| AAAACAGCTCCGCGAGATTC+GGG | - | Chr1:98616085-98616104 | None:intergenic | 50.0% | |
| ATGTTCAATCCACCGGCAAG+AGG | + | Chr1:98616619-98616638 | MsG0180005997.01.T01:CDS | 50.0% | |
| CAAAACAGCTCCGCGAGATT+CGG | - | Chr1:98616086-98616105 | None:intergenic | 50.0% | |
| CAGCAGAGGTTGTTGAACTG+AGG | - | Chr1:98616555-98616574 | None:intergenic | 50.0% | |
| GAAGCTGCATAGAGACCCTT+TGG | - | Chr1:98616958-98616977 | None:intergenic | 50.0% | |
| GACTCTCTTTGTCTCGTGTG+TGG | + | Chr1:98616906-98616925 | MsG0180005997.01.T01:CDS | 50.0% | |
| GGAAGAAGCTGAAATGGTGG+AGG | + | Chr1:98617341-98617360 | MsG0180005997.01.T01:CDS | 50.0% | |
| TAATCATCACCTCTTGCCGG+TGG | - | Chr1:98616631-98616650 | None:intergenic | 50.0% | |
| ! | AGCAATGTTGGTGTCCTTGC+AGG | + | Chr1:98615854-98615873 | MsG0180005997.01.T01:CDS | 50.0% |
| !! | GAATCTCGCGGAGCTGTTTT+GGG | + | Chr1:98616085-98616104 | MsG0180005997.01.T01:CDS | 50.0% |
| AAACAGCTCCGCGAGATTCG+GGG | - | Chr1:98616084-98616103 | None:intergenic | 55.0% | |
| ACCGAACATGTAGGTGGCTC+CGG | - | Chr1:98615753-98615772 | None:intergenic | 55.0% | |
| ! | CGAATCTCGCGGAGCTGTTT+TGG | + | Chr1:98616084-98616103 | MsG0180005997.01.T01:CDS | 55.0% |
| CTCACCGTCCAAGCCATCAC+CGG | + | Chr1:98615683-98615702 | MsG0180005997.01.T01:CDS | 60.0% | |
| GACAAACCACCGTCCGGTGA+TGG | - | Chr1:98615699-98615718 | None:intergenic | 60.0% | |
| GCCGGAGCCACCTACATGTT+CGG | + | Chr1:98615749-98615768 | MsG0180005997.01.T01:CDS | 60.0% | |
| CCGTCCAAGCCATCACCGGA+CGG | + | Chr1:98615687-98615706 | MsG0180005997.01.T01:CDS | 65.0% | |
| TCCAAGCCATCACCGGACGG+TGG | + | Chr1:98615690-98615709 | MsG0180005997.01.T01:CDS | 65.0% | |
| ! | ACCACCGTCCGGTGATGGCT+TGG | - | Chr1:98615694-98615713 | None:intergenic | 65.0% |
| ! | CCGTCCGGTGATGGCTTGGA+CGG | - | Chr1:98615690-98615709 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01;Name=MsG0180005997.01 |
| Chr1 | mRNA | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01;Parent=MsG0180005997.01;Name=MsG0180005997.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|589 |
| Chr1 | exon | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01:exon:19725;Parent=MsG0180005997.01.T01 |
| Chr1 | CDS | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01:cds;Parent=MsG0180005997.01.T01 |
| Gene Sequence |
| Protein sequence |
