Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | RHN82474.1 | 95.4 | 587 | 20 | 1 | 1 | 587 | 601 | 1180 | 0 | 1129 |
| MsG0180005997.01.T01 | RHN82474.1 | 89.492 | 590 | 53 | 2 | 2 | 589 | 1195 | 1777 | 0 | 1082 |
| MsG0180005997.01.T01 | RHN82474.1 | 88.983 | 590 | 56 | 2 | 2 | 589 | 6 | 588 | 0 | 1072 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | A0A396JZ33 | 95.400 | 587 | 20 | 1 | 1 | 587 | 601 | 1180 | 0.0 | 1129 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0180005997.01 | 0.814846 | 1.251335e-51 | 8.489231e-49 |
| MsG0180001050.01 | MsG0180005997.01 | 0.838649 | 2.537076e-57 | 3.420297e-54 |
| MsG0180005996.01 | MsG0180005997.01 | 0.842081 | 3.215300e-58 | 4.820581e-55 |
| MsG0180005997.01 | MsG0280011053.01 | 0.841804 | 3.805112e-58 | 5.654832e-55 |
| MsG0180005997.01 | MsG0280011152.01 | 0.819262 | 1.273933e-52 | 9.755199e-50 |
| MsG0180005997.01 | MsG0280011350.01 | 0.805532 | 1.275920e-49 | 6.766366e-47 |
| MsG0180005997.01 | MsG0380011613.01 | 0.824724 | 6.910596e-54 | 6.172597e-51 |
| MsG0180005997.01 | MsG0380016644.01 | 0.839591 | 1.446282e-57 | 2.006601e-54 |
| MsG0180005997.01 | MsG0480018904.01 | 0.802384 | 5.756444e-49 | 2.816075e-46 |
| MsG0180005997.01 | MsG0480019106.01 | 0.802740 | 4.860903e-49 | 2.399553e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | MTR_1g111530 | 94.719 | 587 | 26 | 1 | 1 | 587 | 1 | 582 | 0.0 | 1104 |
| MsG0180005997.01.T01 | MTR_1g111540 | 89.456 | 588 | 57 | 1 | 2 | 589 | 6 | 588 | 0.0 | 1065 |
| MsG0180005997.01.T01 | MTR_1g111510 | 89.116 | 588 | 59 | 1 | 2 | 589 | 6 | 588 | 0.0 | 1055 |
| MsG0180005997.01.T01 | MTR_1g111550 | 71.601 | 581 | 151 | 3 | 12 | 584 | 15 | 589 | 0.0 | 853 |
| MsG0180005997.01.T01 | MTR_7g102880 | 60.302 | 597 | 213 | 6 | 5 | 584 | 12 | 601 | 0.0 | 710 |
| MsG0180005997.01.T01 | MTR_1g050550 | 58.065 | 589 | 237 | 4 | 6 | 584 | 8 | 596 | 0.0 | 699 |
| MsG0180005997.01.T01 | MTR_1g059080 | 57.168 | 572 | 232 | 4 | 6 | 573 | 8 | 570 | 0.0 | 669 |
| MsG0180005997.01.T01 | MTR_2g100400 | 34.261 | 575 | 312 | 8 | 19 | 567 | 4 | 538 | 1.61e-106 | 331 |
| MsG0180005997.01.T01 | MTR_5g090570 | 34.054 | 555 | 325 | 8 | 22 | 567 | 11 | 533 | 2.15e-103 | 323 |
| MsG0180005997.01.T01 | MTR_7g029015 | 34.783 | 575 | 323 | 11 | 22 | 557 | 15 | 576 | 2.87e-101 | 319 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005997.01.T01 | AT2G39210 | 71.658 | 561 | 156 | 2 | 9 | 568 | 8 | 566 | 0.0 | 841 |
| MsG0180005997.01.T01 | AT2G28120 | 61.441 | 555 | 208 | 1 | 16 | 570 | 11 | 559 | 0.0 | 699 |
| MsG0180005997.01.T01 | AT1G74780 | 36.219 | 566 | 303 | 7 | 22 | 568 | 7 | 533 | 3.43e-114 | 351 |
| MsG0180005997.01.T01 | AT1G18940 | 36.071 | 560 | 299 | 8 | 14 | 558 | 1 | 516 | 8.97e-110 | 339 |
| MsG0180005997.01.T01 | AT2G34355 | 34.271 | 569 | 305 | 8 | 16 | 564 | 1 | 520 | 2.59e-103 | 322 |
| MsG0180005997.01.T01 | AT2G34350 | 34.107 | 560 | 308 | 8 | 22 | 564 | 7 | 522 | 3.26e-98 | 309 |
| MsG0180005997.01.T01 | AT2G34350 | 34.107 | 560 | 308 | 8 | 22 | 564 | 7 | 522 | 3.26e-98 | 309 |
| MsG0180005997.01.T01 | AT5G50520 | 33.455 | 547 | 327 | 7 | 19 | 557 | 13 | 530 | 1.05e-96 | 306 |
| MsG0180005997.01.T01 | AT5G50630 | 33.455 | 547 | 327 | 7 | 19 | 557 | 13 | 530 | 1.05e-96 | 306 |
| MsG0180005997.01.T01 | AT5G14120 | 33.507 | 576 | 351 | 9 | 16 | 566 | 10 | 578 | 1.73e-96 | 306 |
Find 99 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGCTCCGCGAGATTC+GGG | 0.368427 | 1:-98616082 | None:intergenic |
| AAAATGTTCTGAAACAAATC+CGG | 0.495163 | 1:-98616842 | None:intergenic |
| AAACAGCTCCGCGAGATTCG+GGG | 0.632787 | 1:-98616081 | None:intergenic |
| AAACTGCAAGACCACATGTT+TGG | 0.447304 | 1:+98615975 | MsG0180005997.01.T01:CDS |
| AAAGAAAGCATAAGAGGACG+AGG | 0.591288 | 1:-98616883 | None:intergenic |
| AAAGCATAGAATAGAAGGGC+AGG | 0.553260 | 1:+98617176 | MsG0180005997.01.T01:CDS |
| AAAGCGAATGAAATAGCAGC+AGG | 0.578973 | 1:-98616214 | None:intergenic |
| AAAGGATATCTTGGTCTTAG+TGG | 0.440413 | 1:+98616115 | MsG0180005997.01.T01:CDS |
| AACACAAGTAACAAGTGAAC+CGG | 0.486587 | 1:-98616050 | None:intergenic |
| AACAGGTTTCATATACCGAA+TGG | 0.597164 | 1:-98616245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGACATGTTGATACTATTTG+TGG | + | Chr1:98616678-98616697 | MsG0180005997.01.T01:CDS | 30.0% | |
| ATGTGTCTTTACATTTGCAT+AGG | + | Chr1:98616001-98616020 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAAAAAAAGATCCAAAATGG+TGG | + | Chr1:98616590-98616609 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAAAGAAGCTAAGAAACAAT+TGG | + | Chr1:98617143-98617162 | MsG0180005997.01.T01:CDS | 30.0% | |
| CAATAAAAACAACAGCAAGT+GGG | - | Chr1:98616429-98616448 | None:intergenic | 30.0% | |
| GTGACATATACAAGAGATAT+AGG | + | Chr1:98617319-98617338 | MsG0180005997.01.T01:CDS | 30.0% | |
| TCAATAAAAACAACAGCAAG+TGG | - | Chr1:98616430-98616449 | None:intergenic | 30.0% | |
| TCCCAAAAAAAGATCCAAAA+TGG | + | Chr1:98616587-98616606 | MsG0180005997.01.T01:CDS | 30.0% | |
| TCTTTGGTTACTTCATGATT+TGG | + | Chr1:98615936-98615955 | MsG0180005997.01.T01:CDS | 30.0% | |
| TTAAGCACATATAAGCCAAT+TGG | - | Chr1:98617096-98617115 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01;Name=MsG0180005997.01 |
| Chr1 | mRNA | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01;Parent=MsG0180005997.01;Name=MsG0180005997.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|589 |
| Chr1 | exon | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01:exon:19725;Parent=MsG0180005997.01.T01 |
| Chr1 | CDS | 98615644 | 98617413 | 98615644 | ID=MsG0180005997.01.T01:cds;Parent=MsG0180005997.01.T01 |
| Gene Sequence |
>MsG0180005997.01.T01 ATGGCAATTGGTGTTCATGATTGGGGAGACATGAAGAGTCTCACCGTCCAAGCCATCACCGGACGGTGGTTTGTCATTTTTGCTTCCTTTCTAATCATGTCAGCTGCCGGAGCCACCTACATGTTCGGTCTATACTCCAGCGACATAAAAGAAACACTTGGCTATGACCAAAGCACACTAGACCTCCTTAGTTTTTTCAAAGATTTTGGTAGCAATGTTGGTGTCCTTGCAGGACTAATCAACGAAATCTCTCCACCTTGGGTAGTTTTAGCAATAGGTGCTATACTTAATTTCTTTGGTTACTTCATGATTTGGCTATCCATAACAAAAAAAACTGCAAGACCACATGTTTGGCAAATGTGTCTTTACATTTGCATAGGTGCTAATTCTCAGTCTTTTGCAAACACCGGTTCACTTGTTACTTGTGTTAAGAATTTCCCCGAATCTCGCGGAGCTGTTTTGGGAATTCTAAAAGGATATCTTGGTCTTAGTGGTGCTATCATCACACAACTTTACTCTGCTATTTATTATGATGACTCAAAGGCTTTGATTTTGCTTATAGGTTGGCTTCCTGCTGCTATTTCATTCGCTTTTCTTAGAACCATTCGGTATATGAAACCTGTTCGACAAGTCAATGAGCTTAAGGTTTTTTATAATTTCTTGTACATCTCACTTGCTCTTGCTGGTTTCTTGTTGGTTATAATTATTATAGAGAAGAGTAGTAGTTTCACACAGGGTGAGTTTGGTTTAAGTGCTGCTGTGGTGATTTTCTTGCTTTTTCTCCCACTTGCTGTTGTTTTTATTGAAGAGAACAAGATTTGGAAGAGTAAAAAGCTTGCTTTGGTTGACTCTTCACCAGTGCAAATAGTAACAGAAGGTAAAACTGAAACTGAAAAAGCCAACAACAACCTCAGTTCAACAACCTCTGCTGTTTCAGTTTCAGTCCCAAAAAAAGATCCAAAATGGTGGGAAGATATGTTCAATCCACCGGCAAGAGGTGATGATTATACAATACTTCAAGCACTTTTCAGCATAGACATGTTGATACTATTTGTGGCATGTATTTGTGGTATTGGTGGATCATTGACAGCAGTTGATAATCTTGGTCAGATAGGAACATCACTTAGATATCCAAAGAAAAGCATAAGCACTTTTGTGTCTTTAGTAAGTATTTGGAATTATTTAGGACGTGTTTTCTCCGGATTTGTTTCAGAACATTTTCTAACAAAGTACAGATTTCCTCGTCCTCTTATGCTTTCTTTGACTCTCTTTGTCTCGTGTGTGGGACACCTTTTGATAGCATTTGGTGTGCCAAAGGGTCTCTATGCAGCTTCAGTGATTATCGGATTTTGTTTTGGTGCTCAATGGCCATTACTTTTTGCCATTATATCAGAACTTTTTGGACTTAAATATTATGCAACTTTGTATAATTTTGGAAGTATAACAAGTCCAATTGGCTTATATGTGCTTAATGTGAAGATCACTGGTCATTTGTATGACAAAGAAGCTAAGAAACAATTGGAAGCACTTGGAAAGCATAGAATAGAAGGGCAGGAATTGAATTGTGTTGGTGTTGATTGTTTCAAGTTATCTTTCATTATTATTGCAGCTGCAACTTTCTTTGGTGTTGTTGTTAACCTTATTTTGGTGGCTAGGACAAGAAAGTTTTACAAAGGTGACATATACAAGAGATATAGGGAAGAAGCTGAAATGGTGGAGGTAAAGAACGAGGGAAAAACAGAAGAAGATGACAAAGTTGGCATGCGGTGA |
| Protein sequence |
>MsG0180005997.01.T01 MAIGVHDWGDMKSLTVQAITGRWFVIFASFLIMSAAGATYMFGLYSSDIKETLGYDQSTLDLLSFFKDFGSNVGVLAGLINEISPPWVVLAIGAILNFFGYFMIWLSITKKTARPHVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGAVLGILKGYLGLSGAIITQLYSAIYYDDSKALILLIGWLPAAISFAFLRTIRYMKPVRQVNELKVFYNFLYISLALAGFLLVIIIIEKSSSFTQGEFGLSAAVVIFLLFLPLAVVFIEENKIWKSKKLALVDSSPVQIVTEGKTETEKANNNLSSTTSAVSVSVPKKDPKWWEDMFNPPARGDDYTILQALFSIDMLILFVACICGIGGSLTAVDNLGQIGTSLRYPKKSISTFVSLVSIWNYLGRVFSGFVSEHFLTKYRFPRPLMLSLTLFVSCVGHLLIAFGVPKGLYAASVIIGFCFGAQWPLLFAIISELFGLKYYATLYNFGSITSPIGLYVLNVKITGHLYDKEAKKQLEALGKHRIEGQELNCVGVDCFKLSFIIIAAATFFGVVVNLILVARTRKFYKGDIYKRYREEAEMVEVKNEGKTEEDDKVGMR* |
