Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005996.01.T01 | KEH44269.1 | 95.153 | 557 | 16 | 4 | 1 | 553 | 45 | 594 | 0 | 1058 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005996.01.T01 | F4I9E1 | 23.247 | 542 | 383 | 11 | 2 | 537 | 63 | 577 | 1.46E-23 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005996.01.T01 | A0A072VQH4 | 95.135 | 555 | 20 | 3 | 1 | 553 | 45 | 594 | 0.0 | 1040 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0180005996.01 | 0.811283 | 7.566050e-51 | 4.664111e-48 |
| MsG0180000222.01 | MsG0180005996.01 | 0.806614 | 7.551061e-50 | 4.116899e-47 |
| MsG0180000887.01 | MsG0180005996.01 | 0.805573 | 1.250753e-49 | 6.639936e-47 |
| MsG0180001050.01 | MsG0180005996.01 | 0.845688 | 3.475189e-59 | 5.838697e-56 |
| MsG0180002989.01 | MsG0180005996.01 | 0.807754 | 4.330642e-50 | 2.432045e-47 |
| MsG0180003130.01 | MsG0180005996.01 | 0.825558 | 4.389498e-54 | 4.014548e-51 |
| MsG0180004562.01 | MsG0180005996.01 | 0.804515 | 2.081936e-49 | 1.075525e-46 |
| MsG0180005996.01 | MsG0180005997.01 | 0.842081 | 3.215300e-58 | 4.820581e-55 |
| MsG0180005996.01 | MsG0280006433.01 | 0.813882 | 2.043686e-51 | 1.350784e-48 |
| MsG0180005996.01 | MsG0280006581.01 | 0.828041 | 1.120480e-54 | 1.100984e-51 |
| MsG0180005996.01 | MsG0280007272.01 | 0.855256 | 7.126760e-62 | 1.635388e-58 |
| MsG0180005996.01 | MsG0280007369.01 | 0.847036 | 1.490447e-59 | 2.615201e-56 |
| MsG0180005996.01 | MsG0280007391.01 | 0.851549 | 8.257920e-61 | 1.677422e-57 |
| MsG0180005996.01 | MsG0280007751.01 | 0.822326 | 2.515320e-53 | 2.098634e-50 |
| MsG0180005996.01 | MsG0280011053.01 | 0.882514 | 9.776430e-71 | 5.972364e-67 |
| MsG0180005996.01 | MsG0280011152.01 | 0.850402 | 1.737811e-60 | 3.400148e-57 |
| MsG0180005996.01 | MsG0280011350.01 | 0.814464 | 1.520438e-51 | 1.020948e-48 |
| MsG0180005996.01 | MsG0380011613.01 | 0.812006 | 5.266711e-51 | 3.309785e-48 |
| MsG0180005996.01 | MsG0380012607.01 | 0.831806 | 1.354309e-55 | 1.485987e-52 |
| MsG0180005996.01 | MsG0380014920.01 | 0.838656 | 2.526490e-57 | 3.406668e-54 |
| MsG0180005996.01 | MsG0380016598.01 | 0.840523 | 8.261522e-58 | 1.179930e-54 |
| MsG0180005996.01 | MsG0380016644.01 | 0.845178 | 4.775799e-59 | 7.892303e-56 |
| MsG0180005996.01 | MsG0380017158.01 | 0.804316 | 2.290264e-49 | 1.177147e-46 |
| MsG0180005996.01 | MsG0480018500.01 | 0.828471 | 8.824539e-55 | 8.779809e-52 |
| MsG0180005996.01 | MsG0480018904.01 | 0.857230 | 1.880737e-62 | 4.609659e-59 |
| MsG0180005996.01 | MsG0480020219.01 | 0.806838 | 6.771889e-50 | 3.713612e-47 |
| MsG0180005996.01 | MsG0480020444.01 | 0.837586 | 4.762255e-57 | 6.214476e-54 |
| MsG0180005996.01 | MsG0480022117.01 | 0.833564 | 4.959513e-56 | 5.733149e-53 |
| MsG0180005996.01 | MsG0480022125.01 | 0.828532 | 8.533625e-55 | 8.505344e-52 |
| MsG0180005996.01 | MsG0480022507.01 | 0.866875 | 2.070155e-65 | 7.086418e-62 |
| MsG0180005996.01 | MsG0480022508.01 | 0.861518 | 9.695138e-64 | 2.750924e-60 |
| MsG0180005996.01 | MsG0480022680.01 | 0.810479 | 1.129223e-50 | 6.814234e-48 |
| MsG0180005996.01 | MsG0480022736.01 | 0.806200 | 9.233383e-50 | 4.981448e-47 |
| MsG0180005996.01 | MsG0480023903.01 | 0.813837 | 2.090813e-51 | 1.380227e-48 |
| MsG0180005996.01 | MsG0480023947.01 | 0.804680 | 1.923329e-49 | 9.978927e-47 |
| MsG0180005996.01 | MsG0580025180.01 | 0.803633 | 3.176303e-49 | 1.604163e-46 |
| MsG0180005996.01 | MsG0580025876.01 | 0.830835 | 2.347830e-55 | 2.502755e-52 |
| MsG0180005996.01 | MsG0580027227.01 | 0.836648 | 8.274931e-57 | 1.049282e-53 |
| MsG0180005996.01 | MsG0580028051.01 | 0.810840 | 9.437262e-51 | 5.750241e-48 |
| MsG0180005996.01 | MsG0580028416.01 | 0.809755 | 1.617926e-50 | 9.579976e-48 |
| MsG0180005996.01 | MsG0580029906.01 | 0.802899 | 4.506753e-49 | 2.233770e-46 |
| MsG0180005996.01 | MsG0580029930.01 | 0.835284 | 1.834816e-56 | 2.233604e-53 |
| MsG0180005996.01 | MsG0680030559.01 | 0.820265 | 7.516910e-53 | 5.919203e-50 |
| MsG0180005996.01 | MsG0680030996.01 | 0.822077 | 2.873047e-53 | 2.379966e-50 |
| MsG0180005996.01 | MsG0680031429.01 | 0.826173 | 3.136777e-54 | 2.919684e-51 |
| MsG0180005996.01 | MsG0680034852.01 | 0.817334 | 3.479605e-52 | 2.526821e-49 |
| MsG0180005996.01 | MsG0680034919.01 | 0.808583 | 2.884574e-50 | 1.655518e-47 |
| MsG0180005996.01 | MsG0680035496.01 | 0.814126 | 1.805472e-51 | 1.201240e-48 |
| MsG0180005996.01 | MsG0780036514.01 | 0.816442 | 5.519110e-52 | 3.910916e-49 |
| MsG0180005996.01 | MsG0780038123.01 | 0.829401 | 5.255104e-55 | 5.372899e-52 |
| MsG0180005996.01 | MsG0780038444.01 | 0.817736 | 2.825561e-52 | 2.074662e-49 |
| MsG0180005996.01 | MsG0780038515.01 | 0.858353 | 8.735636e-63 | 2.223001e-59 |
| MsG0180005996.01 | MsG0780039450.01 | 0.812937 | 3.297328e-51 | 2.124159e-48 |
| MsG0180005996.01 | MsG0780040060.01 | 0.843891 | 1.059917e-58 | 1.682322e-55 |
| MsG0180005996.01 | MsG0780040609.01 | 0.859925 | 2.952187e-63 | 7.928896e-60 |
| MsG0180005996.01 | MsG0780041589.01 | 0.843703 | 1.190581e-58 | 1.878274e-55 |
| MsG0180005996.01 | MsG0880042320.01 | 0.837648 | 4.591575e-57 | 6.003716e-54 |
| MsG0180005996.01 | MsG0880042368.01 | 0.846511 | 2.075097e-59 | 3.580396e-56 |
| MsG0180005996.01 | MsG0880042843.01 | 0.818894 | 1.544715e-52 | 1.171003e-49 |
| MsG0180005996.01 | MsG0880043094.01 | 0.805346 | 1.395480e-49 | 7.365405e-47 |
| MsG0180005996.01 | MsG0880043824.01 | 0.808243 | 3.408240e-50 | 1.938881e-47 |
| MsG0180005996.01 | MsG0880044669.01 | 0.850841 | 1.308457e-60 | 2.597259e-57 |
| MsG0180005996.01 | MsG0880045861.01 | 0.846205 | 2.513723e-59 | 4.293756e-56 |
| MsG0180005996.01 | MsG0880046586.01 | 0.810155 | 1.326702e-50 | 7.937979e-48 |
| MsG0180005996.01 | MsG0880046975.01 | 0.826776 | 2.253283e-54 | 2.133550e-51 |
| MsG0180005996.01 | MsG0880047081.01 | 0.837420 | 5.252044e-57 | 6.818403e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005996.01.T01 | MTR_1g111510 | 95.153 | 557 | 16 | 4 | 1 | 553 | 45 | 594 | 0.0 | 1058 |
| MsG0180005996.01.T01 | MTR_1g111540 | 92.072 | 555 | 37 | 3 | 1 | 553 | 45 | 594 | 0.0 | 1015 |
| MsG0180005996.01.T01 | MTR_1g111530 | 88.160 | 549 | 58 | 3 | 1 | 547 | 41 | 584 | 0.0 | 966 |
| MsG0180005996.01.T01 | MTR_1g111550 | 72.826 | 552 | 134 | 5 | 1 | 542 | 44 | 589 | 0.0 | 809 |
| MsG0180005996.01.T01 | MTR_7g102880 | 62.633 | 562 | 182 | 8 | 1 | 542 | 48 | 601 | 0.0 | 679 |
| MsG0180005996.01.T01 | MTR_1g050550 | 58.484 | 554 | 218 | 5 | 1 | 542 | 43 | 596 | 0.0 | 659 |
| MsG0180005996.01.T01 | MTR_1g059080 | 57.542 | 537 | 213 | 5 | 1 | 531 | 43 | 570 | 0.0 | 629 |
| MsG0180005996.01.T01 | MTR_7g029015 | 35.432 | 556 | 305 | 11 | 1 | 515 | 34 | 576 | 6.38e-100 | 314 |
| MsG0180005996.01.T01 | MTR_2g100400 | 35.027 | 551 | 292 | 9 | 2 | 525 | 27 | 538 | 8.17e-100 | 313 |
| MsG0180005996.01.T01 | MTR_5g090570 | 32.897 | 535 | 316 | 10 | 2 | 525 | 31 | 533 | 1.75e-94 | 298 |
| MsG0180005996.01.T01 | MTR_3g099150 | 34.622 | 543 | 306 | 11 | 1 | 516 | 34 | 554 | 6.48e-93 | 295 |
| MsG0180005996.01.T01 | MTR_4g116210 | 32.897 | 535 | 333 | 9 | 2 | 516 | 31 | 559 | 2.03e-83 | 271 |
| MsG0180005996.01.T01 | MTR_4g073690 | 33.969 | 524 | 313 | 9 | 2 | 516 | 35 | 534 | 4.88e-83 | 269 |
| MsG0180005996.01.T01 | MTR_5g022380 | 32.156 | 538 | 319 | 9 | 2 | 531 | 33 | 532 | 2.40e-77 | 254 |
| MsG0180005996.01.T01 | MTR_2g450710 | 30.784 | 536 | 336 | 8 | 2 | 516 | 32 | 553 | 7.89e-76 | 251 |
| MsG0180005996.01.T01 | MTR_5g022380 | 30.652 | 460 | 273 | 9 | 80 | 531 | 1 | 422 | 1.32e-57 | 198 |
| MsG0180005996.01.T01 | MTR_4g073690 | 33.155 | 374 | 226 | 8 | 2 | 366 | 35 | 393 | 1.91e-49 | 176 |
| MsG0180005996.01.T01 | MTR_2g104250 | 30.263 | 228 | 117 | 5 | 289 | 516 | 104 | 289 | 1.96e-28 | 115 |
| MsG0180005996.01.T01 | MTR_5g095240 | 67.606 | 71 | 23 | 0 | 452 | 522 | 2 | 72 | 8.46e-27 | 103 |
| MsG0180005996.01.T01 | MTR_7g083660 | 24.864 | 551 | 376 | 15 | 2 | 529 | 27 | 562 | 6.69e-25 | 108 |
| MsG0180005996.01.T01 | MTR_3g466040 | 23.946 | 522 | 355 | 9 | 2 | 516 | 27 | 513 | 3.60e-23 | 103 |
| MsG0180005996.01.T01 | MTR_3g466110 | 23.265 | 490 | 335 | 9 | 2 | 485 | 27 | 481 | 1.24e-22 | 101 |
| MsG0180005996.01.T01 | MTR_5g026350 | 24.508 | 559 | 374 | 14 | 2 | 528 | 27 | 569 | 5.76e-22 | 100 |
| MsG0180005996.01.T01 | MTR_8g071920 | 23.708 | 561 | 380 | 15 | 2 | 530 | 68 | 612 | 5.73e-18 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005996.01.T01 | AT2G39210 | 71.834 | 529 | 144 | 4 | 1 | 526 | 40 | 566 | 0.0 | 775 |
| MsG0180005996.01.T01 | AT2G28120 | 62.101 | 533 | 188 | 4 | 1 | 528 | 36 | 559 | 0.0 | 660 |
| MsG0180005996.01.T01 | AT1G74780 | 35.543 | 543 | 296 | 7 | 2 | 526 | 27 | 533 | 3.78e-102 | 318 |
| MsG0180005996.01.T01 | AT1G18940 | 36.604 | 530 | 279 | 8 | 2 | 516 | 29 | 516 | 1.14e-101 | 317 |
| MsG0180005996.01.T01 | AT5G50520 | 35.795 | 528 | 289 | 10 | 3 | 515 | 38 | 530 | 8.75e-93 | 294 |
| MsG0180005996.01.T01 | AT5G50630 | 35.795 | 528 | 289 | 10 | 3 | 515 | 38 | 530 | 8.75e-93 | 294 |
| MsG0180005996.01.T01 | AT2G34350 | 33.395 | 539 | 298 | 8 | 2 | 522 | 27 | 522 | 5.12e-92 | 292 |
| MsG0180005996.01.T01 | AT2G34350 | 33.395 | 539 | 298 | 8 | 2 | 522 | 27 | 522 | 5.12e-92 | 292 |
| MsG0180005996.01.T01 | AT2G34355 | 32.288 | 542 | 298 | 8 | 2 | 522 | 27 | 520 | 2.40e-91 | 290 |
| MsG0180005996.01.T01 | AT5G14120 | 33.757 | 551 | 331 | 11 | 1 | 524 | 35 | 578 | 1.29e-88 | 285 |
| MsG0180005996.01.T01 | AT3G01930 | 31.738 | 564 | 332 | 9 | 1 | 525 | 35 | 584 | 5.26e-88 | 283 |
| MsG0180005996.01.T01 | AT4G34950 | 32.762 | 525 | 332 | 5 | 2 | 515 | 36 | 550 | 3.79e-87 | 280 |
| MsG0180005996.01.T01 | AT2G16660 | 34.799 | 523 | 305 | 8 | 2 | 515 | 34 | 529 | 6.82e-84 | 271 |
| MsG0180005996.01.T01 | AT1G80530 | 31.734 | 542 | 313 | 13 | 2 | 516 | 33 | 544 | 4.06e-71 | 238 |
| MsG0180005996.01.T01 | AT3G01930 | 31.172 | 478 | 290 | 10 | 80 | 525 | 1 | 471 | 5.71e-66 | 222 |
| MsG0180005996.01.T01 | AT2G34355 | 30.456 | 417 | 229 | 7 | 119 | 522 | 2 | 370 | 1.85e-59 | 202 |
| MsG0180005996.01.T01 | AT3G01930 | 30.348 | 402 | 230 | 7 | 1 | 363 | 35 | 425 | 7.58e-51 | 181 |
| MsG0180005996.01.T01 | AT2G16660 | 34.225 | 374 | 219 | 7 | 2 | 366 | 34 | 389 | 2.00e-49 | 176 |
| MsG0180005996.01.T01 | AT5G45275 | 25.688 | 545 | 360 | 13 | 2 | 516 | 27 | 556 | 2.93e-27 | 116 |
| MsG0180005996.01.T01 | AT4G19450 | 24.309 | 543 | 372 | 13 | 2 | 516 | 27 | 558 | 3.65e-26 | 113 |
| MsG0180005996.01.T01 | AT1G31470 | 23.247 | 542 | 383 | 11 | 2 | 537 | 63 | 577 | 1.49e-24 | 108 |
| MsG0180005996.01.T01 | AT3G01630 | 24.813 | 536 | 348 | 16 | 2 | 516 | 43 | 544 | 3.98e-24 | 106 |
| MsG0180005996.01.T01 | AT3G01630 | 24.813 | 536 | 348 | 16 | 2 | 516 | 61 | 562 | 4.09e-24 | 106 |
| MsG0180005996.01.T01 | AT4G19450 | 24.416 | 385 | 260 | 9 | 2 | 359 | 27 | 407 | 4.29e-18 | 87.0 |
| MsG0180005996.01.T01 | AT2G30300 | 25.701 | 214 | 146 | 4 | 304 | 516 | 296 | 497 | 8.54e-11 | 65.1 |
Find 86 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCACCTTTGTAAAACTTTC+TGG | 0.108023 | 1:-98611640 | None:intergenic |
| AACACATCTTCCCACCATTT+TGG | 0.178261 | 1:-98610943 | None:intergenic |
| TGCTGTTGTTTCACTTATTT+TGG | 0.181515 | 1:+98611608 | MsG0180005996.01.T01:CDS |
| TATGCAACTTTATATAATTT+TGG | 0.182353 | 1:+98611396 | MsG0180005996.01.T01:CDS |
| GGAATCTCGTGGCGCGGTTT+TGG | 0.197076 | 1:+98610429 | MsG0180005996.01.T01:CDS |
| TATATTTCACTTGGTCTTGC+TGG | 0.241800 | 1:+98610652 | MsG0180005996.01.T01:CDS |
| TACGTAGCTTCAGTAATAAT+TGG | 0.273011 | 1:+98611306 | MsG0180005996.01.T01:CDS |
| GGTGCTATTCTCAATTTCTT+TGG | 0.281836 | 1:+98610265 | MsG0180005996.01.T01:CDS |
| AGCAACGTTGGTGTCCTTTC+AGG | 0.282717 | 1:+98610199 | MsG0180005996.01.T01:CDS |
| TCTTTGGTTACTTCATGATT+TGG | 0.300114 | 1:+98610281 | MsG0180005996.01.T01:CDS |
| GTGACATATACAAGAGATAT+AGG | 0.328395 | 1:+98611658 | MsG0180005996.01.T01:CDS |
| AAAGGCTATGTTGGTCTTAG+TGG | 0.334364 | 1:+98610460 | MsG0180005996.01.T01:CDS |
| ATGTGTCTTTACATTTGTAT+AGG | 0.337463 | 1:+98610346 | MsG0180005996.01.T01:CDS |
| AAAACCGCGCCACGAGATTC+CGG | 0.352220 | 1:-98610427 | None:intergenic |
| GTATAATCATTACCTCTTGC+TGG | 0.354254 | 1:-98610973 | None:intergenic |
| GTAAGCATTTGGAATTATCT+AGG | 0.357538 | 1:+98611147 | MsG0180005996.01.T01:CDS |
| TTAACGGCAATTGATAATCT+TGG | 0.358953 | 1:+98611069 | MsG0180005996.01.T01:CDS |
| CTTGGAAAGCAAAGAATAGA+AGG | 0.371411 | 1:+98611510 | MsG0180005996.01.T01:CDS |
| AAATAGCAAGACCACAAGTT+TGG | 0.379762 | 1:+98610320 | MsG0180005996.01.T01:CDS |
| CTATTTGTGGCATGTATTTG+TGG | 0.387438 | 1:+98611033 | MsG0180005996.01.T01:CDS |
| TGGTCTTGCTGGATTCTTGT+TGG | 0.395905 | 1:+98610663 | MsG0180005996.01.T01:CDS |
| AAACCGCGCCACGAGATTCC+GGG | 0.401261 | 1:-98610426 | None:intergenic |
| TTGTGTCTTTAGTAAGCATT+TGG | 0.401481 | 1:+98611136 | MsG0180005996.01.T01:CDS |
| TAAGGAGGTTTAGTGTGCTT+TGG | 0.411770 | 1:-98610156 | None:intergenic |
| GTGGCATGTATTTGTGGTGT+TGG | 0.412454 | 1:+98611039 | MsG0180005996.01.T01:CDS |
| AGATATGTTGATACTATTTG+TGG | 0.416529 | 1:+98611020 | MsG0180005996.01.T01:CDS |
| CAAAGAAGCAAAGAAGCAAT+TGG | 0.418174 | 1:+98611482 | MsG0180005996.01.T01:CDS |
| AGTTCATTAACTTGTCGAAC+AGG | 0.419859 | 1:-98610607 | None:intergenic |
| ATATAGGGAAGAAGCTGAAA+TGG | 0.422458 | 1:+98611674 | MsG0180005996.01.T01:CDS |
| AGAGTTAAAGTAAGCATAAG+AGG | 0.426460 | 1:-98611228 | None:intergenic |
| TCATTGAAGAGAACAAGATT+TGG | 0.432696 | 1:+98610785 | MsG0180005996.01.T01:CDS |
| TTCGACAAGTTAATGAACTT+AGG | 0.451619 | 1:+98610611 | MsG0180005996.01.T01:CDS |
| AACTCTCTTTGTCTCATGTG+TGG | 0.452502 | 1:+98611245 | MsG0180005996.01.T01:CDS |
| TATGATCATAATTCAGAAGA+AGG | 0.453739 | 1:+98610687 | MsG0180005996.01.T01:CDS |
| TGTTGAACTGAGGTTGTTGT+TGG | 0.458257 | 1:-98610884 | None:intergenic |
| TCGACAAGTTAATGAACTTA+GGG | 0.459267 | 1:+98610612 | MsG0180005996.01.T01:CDS |
| TGGTGTTGGTGGAACTTTAA+CGG | 0.460287 | 1:+98611053 | MsG0180005996.01.T01:CDS |
| ACTCTCTTTGTCTCATGTGT+GGG | 0.462025 | 1:+98611246 | MsG0180005996.01.T01:CDS |
| GGAATTCTTAAAGGCTATGT+TGG | 0.464341 | 1:+98610451 | MsG0180005996.01.T01:CDS |
| ATTGATAATCTTGGTCAGAT+AGG | 0.465696 | 1:+98611078 | MsG0180005996.01.T01:CDS |
| CCTTCTGTTACTATTTGCAC+TGG | 0.477008 | 1:-98610841 | None:intergenic |
| TGACATATACAAGAGATATA+GGG | 0.477639 | 1:+98611659 | MsG0180005996.01.T01:CDS |
| GCCGGAGCCACCTACATGTT+CGG | 0.490142 | 1:+98610094 | None:intergenic |
| GGAAGTGTAGCAAGTCCACT+TGG | 0.498928 | 1:+98611417 | MsG0180005996.01.T01:CDS |
| TACTTGTGTTAAGAATTTCC+CGG | 0.506545 | 1:+98610408 | MsG0180005996.01.T01:CDS |
| GCATGTATTTGTGGTGTTGG+TGG | 0.506795 | 1:+98611042 | MsG0180005996.01.T01:CDS |
| AAAGCAAAGGAAATAGCAGC+AGG | 0.514488 | 1:-98610559 | None:intergenic |
| AATTTCTTGTATATTTCACT+TGG | 0.520204 | 1:+98610643 | MsG0180005996.01.T01:CDS |
| ACAGAAGAAGATGACAAAGT+TGG | 0.536104 | 1:+98611720 | MsG0180005996.01.T01:CDS |
| GTTACTATTTGCACTGGTGA+AGG | 0.536206 | 1:-98610835 | None:intergenic |
| AAGAAGCAATTGGAAGCACT+TGG | 0.537705 | 1:+98611492 | MsG0180005996.01.T01:CDS |
| AACAGGTTTCATATATCGAA+TGG | 0.538846 | 1:-98610590 | None:intergenic |
| AAGAATTTCCCGGAATCTCG+TGG | 0.541146 | 1:+98610418 | MsG0180005996.01.T01:CDS |
| TAGGGAAGAAGCTGAAATGG+TGG | 0.542633 | 1:+98611677 | MsG0180005996.01.T01:CDS |
| TTCAATGAAACAACGGCAAG+TGG | 0.543869 | 1:-98610770 | None:intergenic |
| GTATGGTTTAAGTGCTGCTG+TGG | 0.562592 | 1:+98610729 | MsG0180005996.01.T01:CDS |
| AAAGCAAAGAATAGAAGGGC+AGG | 0.570654 | 1:+98611515 | MsG0180005996.01.T01:CDS |
| TAATCATTACCTCTTGCTGG+TGG | 0.575464 | 1:-98610970 | None:intergenic |
| GGGCAGGAATTGAATTGTGT+TGG | 0.581328 | 1:+98611531 | MsG0180005996.01.T01:CDS |
| GTTCGTTGATTAGTCCTGAA+AGG | 0.581835 | 1:-98610213 | None:intergenic |
| GAAGCTACGTAGAGACCCTC+AGG | 0.585366 | 1:-98611294 | None:intergenic |
| AGACACATTTGCCAAACTTG+TGG | 0.585563 | 1:-98610331 | None:intergenic |
| AAAGACTTTGGTAGCAACGT+TGG | 0.591191 | 1:+98610187 | MsG0180005996.01.T01:CDS |
| AAAGTAAGCATAAGAGGACG+AGG | 0.591288 | 1:-98611222 | None:intergenic |
| GTGCTTAATGTGAAGATCGC+TGG | 0.592619 | 1:+98611447 | MsG0180005996.01.T01:CDS |
| GGAAGAAGCTGAAATGGTGG+AGG | 0.594474 | 1:+98611680 | MsG0180005996.01.T01:CDS |
| GATAGCATTTGATGTGCCTG+AGG | 0.605002 | 1:+98611278 | MsG0180005996.01.T01:CDS |
| ATAGCATTTGATGTGCCTGA+GGG | 0.610223 | 1:+98611279 | MsG0180005996.01.T01:CDS |
| TGTTCTCTTCAATGAAACAA+CGG | 0.610977 | 1:-98610777 | None:intergenic |
| AATGGTTCGAAGAAAAGCAA+AGG | 0.619783 | 1:-98610572 | None:intergenic |
| GTGTTCAATCCACCAGCAAG+AGG | 0.623056 | 1:+98610961 | MsG0180005996.01.T01:CDS |
| GCACATCAAATGCTATCAGA+AGG | 0.624152 | 1:-98611272 | None:intergenic |
| CCTATTGTCAAAACTACCCA+TGG | 0.627938 | 1:-98610244 | None:intergenic |
| GAAGTGTAGCAAGTCCACTT+GGG | 0.630096 | 1:+98611418 | MsG0180005996.01.T01:CDS |
| CAACGAACTCTATCCACCAT+GGG | 0.634367 | 1:+98610228 | MsG0180005996.01.T01:CDS |
| CCAGTGCAAATAGTAACAGA+AGG | 0.634763 | 1:+98610841 | MsG0180005996.01.T01:CDS |
| ATGGTGGAGGTAAAGAACGA+GGG | 0.640025 | 1:+98611693 | MsG0180005996.01.T01:CDS |
| CAGCAGAGGTTGTTGAACTG+AGG | 0.642991 | 1:-98610894 | None:intergenic |
| TCAACGAACTCTATCCACCA+TGG | 0.645821 | 1:+98610227 | MsG0180005996.01.T01:CDS |
| TTGGAAAGCAAAGAATAGAA+GGG | 0.645826 | 1:+98611511 | MsG0180005996.01.T01:CDS |
| TTTCCCGGAATCTCGTGGCG+CGG | 0.648437 | 1:+98610423 | MsG0180005996.01.T01:CDS |
| ATTGTCAAAACTACCCATGG+TGG | 0.655386 | 1:-98610241 | None:intergenic |
| TTAAGCACATATAACCCAAG+TGG | 0.655955 | 1:-98611432 | None:intergenic |
| TATTTACTATGATGACACAA+AGG | 0.659190 | 1:+98610510 | MsG0180005996.01.T01:CDS |
| GACTGAAACTGAAACAGCAG+AGG | 0.678973 | 1:-98610908 | None:intergenic |
| AATGGTGGAGGTAAAGAACG+AGG | 0.714318 | 1:+98611692 | MsG0180005996.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGCAACTTTATATAATTT+TGG | + | Chr1:98611396-98611415 | MsG0180005996.01.T01:CDS | 15.0% |
| !! | AATTTCTTGTATATTTCACT+TGG | + | Chr1:98610643-98610662 | MsG0180005996.01.T01:CDS | 20.0% |
| !!! | GTAATAATTGGATTTTGTTT+TGG | + | Chr1:98611318-98611337 | MsG0180005996.01.T01:CDS | 20.0% |
| ! | AAAAAAAGATCCAAAATGGT+GGG | + | Chr1:98610933-98610952 | MsG0180005996.01.T01:CDS | 25.0% |
| ! | AGATATGTTGATACTATTTG+TGG | + | Chr1:98611020-98611039 | MsG0180005996.01.T01:CDS | 25.0% |
| ! | ATGTGTCTTTACATTTGTAT+AGG | + | Chr1:98610346-98610365 | MsG0180005996.01.T01:CDS | 25.0% |
| ! | GATATAATGGCAAAAAGTAA+TGG | - | Chr1:98611354-98611373 | None:intergenic | 25.0% |
| ! | TATGATCATAATTCAGAAGA+AGG | + | Chr1:98610687-98610706 | MsG0180005996.01.T01:CDS | 25.0% |
| ! | TATTTACTATGATGACACAA+AGG | + | Chr1:98610510-98610529 | MsG0180005996.01.T01:CDS | 25.0% |
| ! | TGACATATACAAGAGATATA+GGG | + | Chr1:98611659-98611678 | MsG0180005996.01.T01:CDS | 25.0% |
| !! | ACCATTTTGGATCTTTTTTT+GGG | - | Chr1:98610933-98610952 | None:intergenic | 25.0% |
| !! | CAAAGTCTTTGAAAAAACTA+AGG | - | Chr1:98610177-98610196 | None:intergenic | 25.0% |
| !! | CCATTATATCAGAACTTTTT+GGG | + | Chr1:98611364-98611383 | MsG0180005996.01.T01:CDS | 25.0% |
| !!! | AAGGCTTTGATTTTGTTTAT+AGG | + | Chr1:98610529-98610548 | MsG0180005996.01.T01:CDS | 25.0% |
| !!! | CTTAGTTTTTTCAAAGACTT+TGG | + | Chr1:98610175-98610194 | MsG0180005996.01.T01:CDS | 25.0% |
| !!! | CTTTGATTTTGTTTATAGGT+TGG | + | Chr1:98610533-98610552 | MsG0180005996.01.T01:CDS | 25.0% |
| AACAGGTTTCATATATCGAA+TGG | - | Chr1:98610593-98610612 | None:intergenic | 30.0% | |
| AGAGTTAAAGTAAGCATAAG+AGG | - | Chr1:98611231-98611250 | None:intergenic | 30.0% | |
| CAAAAAAAGATCCAAAATGG+TGG | + | Chr1:98610932-98610951 | MsG0180005996.01.T01:CDS | 30.0% | |
| GTGACATATACAAGAGATAT+AGG | + | Chr1:98611658-98611677 | MsG0180005996.01.T01:CDS | 30.0% | |
| TACGTAGCTTCAGTAATAAT+TGG | + | Chr1:98611306-98611325 | MsG0180005996.01.T01:CDS | 30.0% | |
| TACTTGTGTTAAGAATTTCC+CGG | + | Chr1:98610408-98610427 | MsG0180005996.01.T01:CDS | 30.0% | |
| TCATTGAAGAGAACAAGATT+TGG | + | Chr1:98610785-98610804 | MsG0180005996.01.T01:CDS | 30.0% | |
| TCCCAAAAAAAGATCCAAAA+TGG | + | Chr1:98610929-98610948 | MsG0180005996.01.T01:CDS | 30.0% | |
| TCGACAAGTTAATGAACTTA+GGG | + | Chr1:98610612-98610631 | MsG0180005996.01.T01:CDS | 30.0% | |
| TCTTTGGTTACTTCATGATT+TGG | + | Chr1:98610281-98610300 | MsG0180005996.01.T01:CDS | 30.0% | |
| TGTTCTCTTCAATGAAACAA+CGG | - | Chr1:98610780-98610799 | None:intergenic | 30.0% | |
| TTCGACAAGTTAATGAACTT+AGG | + | Chr1:98610611-98610630 | MsG0180005996.01.T01:CDS | 30.0% | |
| TTGGAAAGCAAAGAATAGAA+GGG | + | Chr1:98611511-98611530 | MsG0180005996.01.T01:CDS | 30.0% | |
| TTGTGTCTTTAGTAAGCATT+TGG | + | Chr1:98611136-98611155 | MsG0180005996.01.T01:CDS | 30.0% | |
| ! | AGTCTTTGAAAAAACTAAGG+AGG | - | Chr1:98610174-98610193 | None:intergenic | 30.0% |
| ! | ATTGATAATCTTGGTCAGAT+AGG | + | Chr1:98611078-98611097 | MsG0180005996.01.T01:CDS | 30.0% |
| ! | CACCATTTTGGATCTTTTTT+TGG | - | Chr1:98610934-98610953 | None:intergenic | 30.0% |
| ! | CCCAAAAAGTTCTGATATAA+TGG | - | Chr1:98611367-98611386 | None:intergenic | 30.0% |
| ! | GCCATTATATCAGAACTTTT+TGG | + | Chr1:98611363-98611382 | MsG0180005996.01.T01:CDS | 30.0% |
| ! | GTAAGCATTTGGAATTATCT+AGG | + | Chr1:98611147-98611166 | MsG0180005996.01.T01:CDS | 30.0% |
| ! | TTAACGGCAATTGATAATCT+TGG | + | Chr1:98611069-98611088 | MsG0180005996.01.T01:CDS | 30.0% |
| !! | AAAGTGCTTATGCTTTTCTT+TGG | - | Chr1:98611117-98611136 | None:intergenic | 30.0% |
| !!! | AAGTGTTGTTTTTATGTCTG+GGG | - | Chr1:98610128-98610147 | None:intergenic | 30.0% |
| !!! | ATTACAGCTGCAACATTTTT+TGG | + | Chr1:98611585-98611604 | MsG0180005996.01.T01:CDS | 30.0% |
| !!! | CAAGTGTTGTTTTTATGTCT+GGG | - | Chr1:98610129-98610148 | None:intergenic | 30.0% |
| !!! | TGCTGTTGTTTCACTTATTT+TGG | + | Chr1:98611608-98611627 | MsG0180005996.01.T01:CDS | 30.0% |
| !!! | TGTTGTTTCACTTATTTTGG+TGG | + | Chr1:98611611-98611630 | MsG0180005996.01.T01:CDS | 30.0% |
| !!! | TTGTGGTCTTGCTATTTTTT+TGG | - | Chr1:98610317-98610336 | None:intergenic | 30.0% |
| AAATAGCAAGACCACAAGTT+TGG | + | Chr1:98610320-98610339 | MsG0180005996.01.T01:CDS | 35.0% | |
| AATGGTTCGAAGAAAAGCAA+AGG | - | Chr1:98610575-98610594 | None:intergenic | 35.0% | |
| ACAGAAGAAGATGACAAAGT+TGG | + | Chr1:98611720-98611739 | MsG0180005996.01.T01:CDS | 35.0% | |
| ATATAGGGAAGAAGCTGAAA+TGG | + | Chr1:98611674-98611693 | MsG0180005996.01.T01:CDS | 35.0% | |
| CAAAGAAGCAAAGAAGCAAT+TGG | + | Chr1:98611482-98611501 | MsG0180005996.01.T01:CDS | 35.0% | |
| CCAGACATAAAAACAACACT+TGG | + | Chr1:98610127-98610146 | MsG0180005996.01.T01:CDS | 35.0% | |
| CTATTTGTGGCATGTATTTG+TGG | + | Chr1:98611033-98611052 | MsG0180005996.01.T01:CDS | 35.0% | |
| CTTGGAAAGCAAAGAATAGA+AGG | + | Chr1:98611510-98611529 | MsG0180005996.01.T01:CDS | 35.0% | |
| GGAATTCTTAAAGGCTATGT+TGG | + | Chr1:98610451-98610470 | MsG0180005996.01.T01:CDS | 35.0% | |
| GTATAATCATTACCTCTTGC+TGG | - | Chr1:98610976-98610995 | None:intergenic | 35.0% | |
| GTCACCTTTGTAAAACTTTC+TGG | - | Chr1:98611643-98611662 | None:intergenic | 35.0% | |
| TTAAGCACATATAACCCAAG+TGG | - | Chr1:98611435-98611454 | None:intergenic | 35.0% | |
| ! | AGGACCAGAAAGTTTTACAA+AGG | + | Chr1:98611636-98611655 | MsG0180005996.01.T01:CDS | 35.0% |
| ! | AGTTTTTCACAGAGTGAGTA+TGG | + | Chr1:98610712-98610731 | MsG0180005996.01.T01:CDS | 35.0% |
| ! | GCAGAGTAAAAAACTTGCTT+TGG | + | Chr1:98610807-98610826 | MsG0180005996.01.T01:CDS | 35.0% |
| !! | AGTTCATTAACTTGTCGAAC+AGG | - | Chr1:98610610-98610629 | None:intergenic | 35.0% |
| !! | GGTGCTATTCTCAATTTCTT+TGG | + | Chr1:98610265-98610284 | MsG0180005996.01.T01:CDS | 35.0% |
| !! | TATATTTCACTTGGTCTTGC+TGG | + | Chr1:98610652-98610671 | MsG0180005996.01.T01:CDS | 35.0% |
| !!! | CCAAGTGTTGTTTTTATGTC+TGG | - | Chr1:98610130-98610149 | None:intergenic | 35.0% |
| !!! | GATTTTGTTTTGGTGCTCAA+TGG | + | Chr1:98611328-98611347 | MsG0180005996.01.T01:CDS | 35.0% |
| !!! | TTTCACTTATTTTGGTGGCT+AGG | + | Chr1:98611616-98611635 | MsG0180005996.01.T01:CDS | 35.0% |
| AAAGCAAAGAATAGAAGGGC+AGG | + | Chr1:98611515-98611534 | MsG0180005996.01.T01:CDS | 40.0% | |
| AAAGCAAAGGAAATAGCAGC+AGG | - | Chr1:98610562-98610581 | None:intergenic | 40.0% | |
| AAAGTAAGCATAAGAGGACG+AGG | - | Chr1:98611225-98611244 | None:intergenic | 40.0% | |
| AACTCTCTTTGTCTCATGTG+TGG | + | Chr1:98611245-98611264 | MsG0180005996.01.T01:CDS | 40.0% | |
| ACTCTCTTTGTCTCATGTGT+GGG | + | Chr1:98611246-98611265 | MsG0180005996.01.T01:CDS | 40.0% | |
| AGACACATTTGCCAAACTTG+TGG | - | Chr1:98610334-98610353 | None:intergenic | 40.0% | |
| ATAGCATTTGATGTGCCTGA+GGG | + | Chr1:98611279-98611298 | MsG0180005996.01.T01:CDS | 40.0% | |
| ATTGTCAAAACTACCCATGG+TGG | - | Chr1:98610244-98610263 | None:intergenic | 40.0% | |
| CCAGTGCAAATAGTAACAGA+AGG | + | Chr1:98610841-98610860 | MsG0180005996.01.T01:CDS | 40.0% | |
| CCTATTGTCAAAACTACCCA+TGG | - | Chr1:98610247-98610266 | None:intergenic | 40.0% | |
| CCTTCTGTTACTATTTGCAC+TGG | - | Chr1:98610844-98610863 | None:intergenic | 40.0% | |
| GCACATCAAATGCTATCAGA+AGG | - | Chr1:98611275-98611294 | None:intergenic | 40.0% | |
| TAAGGAGGTTTAGTGTGCTT+TGG | - | Chr1:98610159-98610178 | None:intergenic | 40.0% | |
| TAATCATTACCTCTTGCTGG+TGG | - | Chr1:98610973-98610992 | None:intergenic | 40.0% | |
| TGTTGAACTGAGGTTGTTGT+TGG | - | Chr1:98610887-98610906 | None:intergenic | 40.0% | |
| TTCAATGAAACAACGGCAAG+TGG | - | Chr1:98610773-98610792 | None:intergenic | 40.0% | |
| ! | AAAGACTTTGGTAGCAACGT+TGG | + | Chr1:98610187-98610206 | MsG0180005996.01.T01:CDS | 40.0% |
| ! | AACACATCTTCCCACCATTT+TGG | - | Chr1:98610946-98610965 | None:intergenic | 40.0% |
| ! | GTTACTATTTGCACTGGTGA+AGG | - | Chr1:98610838-98610857 | None:intergenic | 40.0% |
| ! | GTTCGTTGATTAGTCCTGAA+AGG | - | Chr1:98610216-98610235 | None:intergenic | 40.0% |
| ! | TCTCAGTCTTTTGCAAACAC+TGG | + | Chr1:98610376-98610395 | MsG0180005996.01.T01:CDS | 40.0% |
| !! | AAAGGCTATGTTGGTCTTAG+TGG | + | Chr1:98610460-98610479 | MsG0180005996.01.T01:CDS | 40.0% |
| !! | AAGAAGCAATTGGAAGCACT+TGG | + | Chr1:98611492-98611511 | MsG0180005996.01.T01:CDS | 40.0% |
| !! | TATCTAGGACGTGTTTTCTC+AGG | + | Chr1:98611162-98611181 | MsG0180005996.01.T01:CDS | 40.0% |
| !! | TGGTGTTGGTGGAACTTTAA+CGG | + | Chr1:98611053-98611072 | MsG0180005996.01.T01:CDS | 40.0% |
| !!! | CCATGGGTAGTTTTGACAAT+AGG | + | Chr1:98610244-98610263 | MsG0180005996.01.T01:CDS | 40.0% |
| !!! | GCGGTTTTGGGAATTCTTAA+AGG | + | Chr1:98610442-98610461 | MsG0180005996.01.T01:CDS | 40.0% |
| AAGAATTTCCCGGAATCTCG+TGG | + | Chr1:98610418-98610437 | MsG0180005996.01.T01:CDS | 45.0% | |
| CAACGAACTCTATCCACCAT+GGG | + | Chr1:98610228-98610247 | MsG0180005996.01.T01:CDS | 45.0% | |
| GAAGTGTAGCAAGTCCACTT+GGG | + | Chr1:98611418-98611437 | MsG0180005996.01.T01:CDS | 45.0% | |
| GACTGAAACTGAAACAGCAG+AGG | - | Chr1:98610911-98610930 | None:intergenic | 45.0% | |
| GATAGCATTTGATGTGCCTG+AGG | + | Chr1:98611278-98611297 | MsG0180005996.01.T01:CDS | 45.0% | |
| GGGCAGGAATTGAATTGTGT+TGG | + | Chr1:98611531-98611550 | MsG0180005996.01.T01:CDS | 45.0% | |
| GTGCTTAATGTGAAGATCGC+TGG | + | Chr1:98611447-98611466 | MsG0180005996.01.T01:CDS | 45.0% | |
| GTGGCATGTATTTGTGGTGT+TGG | + | Chr1:98611039-98611058 | MsG0180005996.01.T01:CDS | 45.0% | |
| TAGGGAAGAAGCTGAAATGG+TGG | + | Chr1:98611677-98611696 | MsG0180005996.01.T01:CDS | 45.0% | |
| TCAACGAACTCTATCCACCA+TGG | + | Chr1:98610227-98610246 | MsG0180005996.01.T01:CDS | 45.0% | |
| ! | AATGGTGGAGGTAAAGAACG+AGG | + | Chr1:98611692-98611711 | MsG0180005996.01.T01:CDS | 45.0% |
| ! | ATGGTGGAGGTAAAGAACGA+GGG | + | Chr1:98611693-98611712 | MsG0180005996.01.T01:CDS | 45.0% |
| ! | GCATGTATTTGTGGTGTTGG+TGG | + | Chr1:98611042-98611061 | MsG0180005996.01.T01:CDS | 45.0% |
| ! | GTATGGTTTAAGTGCTGCTG+TGG | + | Chr1:98610729-98610748 | MsG0180005996.01.T01:CDS | 45.0% |
| ! | TGGTCTTGCTGGATTCTTGT+TGG | + | Chr1:98610663-98610682 | MsG0180005996.01.T01:CDS | 45.0% |
| CAGCAGAGGTTGTTGAACTG+AGG | - | Chr1:98610897-98610916 | None:intergenic | 50.0% | |
| GGAAGAAGCTGAAATGGTGG+AGG | + | Chr1:98611680-98611699 | MsG0180005996.01.T01:CDS | 50.0% | |
| GTGTTCAATCCACCAGCAAG+AGG | + | Chr1:98610961-98610980 | MsG0180005996.01.T01:CDS | 50.0% | |
| ! | AGCAACGTTGGTGTCCTTTC+AGG | + | Chr1:98610199-98610218 | MsG0180005996.01.T01:CDS | 50.0% |
| ! | GGAAGTGTAGCAAGTCCACT+TGG | + | Chr1:98611417-98611436 | MsG0180005996.01.T01:CDS | 50.0% |
| AAAACCGCGCCACGAGATTC+CGG | - | Chr1:98610430-98610449 | None:intergenic | 55.0% | |
| GAAGCTACGTAGAGACCCTC+AGG | - | Chr1:98611297-98611316 | None:intergenic | 55.0% | |
| ! | GAATCTCGTGGCGCGGTTTT+GGG | + | Chr1:98610430-98610449 | MsG0180005996.01.T01:CDS | 55.0% |
| AAACCGCGCCACGAGATTCC+GGG | - | Chr1:98610429-98610448 | None:intergenic | 60.0% | |
| TTTCCCGGAATCTCGTGGCG+CGG | + | Chr1:98610423-98610442 | MsG0180005996.01.T01:CDS | 60.0% | |
| ! | GGAATCTCGTGGCGCGGTTT+TGG | + | Chr1:98610429-98610448 | MsG0180005996.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 98610109 | 98611770 | 98610109 | ID=MsG0180005996.01;Name=MsG0180005996.01 |
| Chr1 | mRNA | 98610109 | 98611770 | 98610109 | ID=MsG0180005996.01.T01;Parent=MsG0180005996.01;Name=MsG0180005996.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|553 |
| Chr1 | exon | 98610109 | 98611770 | 98610109 | ID=MsG0180005996.01.T01:exon:19724;Parent=MsG0180005996.01.T01 |
| Chr1 | CDS | 98610109 | 98611770 | 98610109 | ID=MsG0180005996.01.T01:cds;Parent=MsG0180005996.01.T01 |
| Gene Sequence |
| Protein sequence |
