AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047081.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047081.01.T01 MTR_4g120760 98.750 160 2 0 1 160 1 160 1.96e-115 323
MsG0880047081.01.T01 MTR_4g120760 98.125 160 3 0 1 160 1 160 7.25e-115 322
MsG0880047081.01.T01 MTR_2g435310 74.843 159 40 0 1 159 1 159 1.11e-89 258
MsG0880047081.01.T01 MTR_2g035100 41.026 156 91 1 1 156 1 155 6.36e-38 127
MsG0880047081.01.T01 MTR_2g035105 39.744 156 93 1 1 156 1 155 1.17e-36 124
MsG0880047081.01.T01 MTR_2g035150 39.744 156 93 1 1 156 1 155 5.47e-36 122
MsG0880047081.01.T01 MTR_2g035120 38.217 157 96 1 1 157 1 156 1.45e-35 121
MsG0880047081.01.T01 MTR_2g035130 38.217 157 96 1 1 157 1 156 4.82e-35 120
MsG0880047081.01.T01 MTR_6g033450 38.217 157 96 1 1 157 1 156 4.08e-34 117
MsG0880047081.01.T01 MTR_4g120970 38.217 157 96 1 1 157 1 156 4.08e-34 117
MsG0880047081.01.T01 MTR_2g035210 34.395 157 102 1 1 157 1 156 4.11e-31 110
MsG0880047081.01.T01 MTR_2g035220 34.395 157 102 1 1 157 1 156 8.77e-31 109
MsG0880047081.01.T01 MTR_2g035320 33.974 156 102 1 1 156 1 155 1.37e-29 106
MsG0880047081.01.T01 MTR_2g035170 33.333 156 103 1 1 156 1 155 3.23e-29 105
MsG0880047081.01.T01 MTR_2g035190 33.121 157 104 1 1 157 1 156 1.17e-28 103
MsG0880047081.01.T01 MTR_4g120950 36.709 158 96 3 1 156 1 156 1.43e-27 101
MsG0880047081.01.T01 MTR_2g035320 31.111 90 62 0 67 156 31 120 4.92e-11 57.4
MsG0880047081.01.T01 MTR_3g055130 29.221 154 101 3 6 155 5 154 5.18e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAACTGTGGTAAGAGCTT+TGG 0.325990 8:+81689639 None:intergenic
TGATTGAAGGGGATCCATTA+GGG 0.331913 8:-81688899 MsG0880047081.01.T01:CDS
CAAACTGTGGTAAGAGCTTT+GGG 0.338115 8:+81689640 None:intergenic
ATGATTGAAGGGGATCCATT+AGG 0.378730 8:-81688900 MsG0880047081.01.T01:CDS
ATTGAAGGAGATGGAGGAGC+TGG 0.382904 8:-81689600 MsG0880047081.01.T01:CDS
GATTCAAGCTTGTCCCCTAA+TGG 0.397124 8:+81688885 None:intergenic
GCTTGAATCTATTGCTTATG+AGG 0.397842 8:-81688871 MsG0880047081.01.T01:CDS
TGGAGGTTGTCTATGTAAAA+TGG 0.399526 8:-81688826 MsG0880047081.01.T01:CDS
AGGAAGATGTAAAGGAAGGA+AGG 0.431562 8:-81688764 MsG0880047081.01.T01:CDS
TGCAAATACACTATGATTGA+AGG 0.436438 8:-81688912 MsG0880047081.01.T01:CDS
GCAAGCAGTTACAAGACTAT+TGG 0.438215 8:-81688804 MsG0880047081.01.T01:CDS
TGCTTATGAGGTGAAGTTTG+AGG 0.442957 8:-81688859 MsG0880047081.01.T01:CDS
AAAGATGTTAGTATAATTGA+AGG 0.444869 8:-81689615 MsG0880047081.01.T01:CDS
AAAGATTGATGTGCTTGATA+AGG 0.454495 8:-81688946 MsG0880047081.01.T01:CDS
ACGCGATGGTGCAACGGTTG+AGG 0.456171 8:+81689701 None:intergenic
AAGTTTGAGGCTACAAATGA+TGG 0.474637 8:-81688846 MsG0880047081.01.T01:CDS
GTTAGTATAATTGAAGGAGA+TGG 0.504523 8:-81689609 MsG0880047081.01.T01:CDS
TGTTAAAGAGGAAGATGTAA+AGG 0.506693 8:-81688772 MsG0880047081.01.T01:CDS
GAAGGAAGGGAAAGTACCAT+TGG 0.512113 8:-81688750 MsG0880047081.01.T01:CDS
CATTGGAATTTACAAAGTTG+TGG 0.512917 8:-81688733 MsG0880047081.01.T01:CDS
GGAAGATGTAAAGGAAGGAA+GGG 0.521833 8:-81688763 MsG0880047081.01.T01:CDS
GAACAAGTCAACTTCAATGA+AGG 0.526221 8:-81689570 MsG0880047081.01.T01:intron
GCAAATACACTATGATTGAA+GGG 0.531266 8:-81688911 MsG0880047081.01.T01:CDS
AGTTGTGGAGTCATATCTGC+TGG 0.538493 8:-81688718 MsG0880047081.01.T01:CDS
AAAGAGGAAGATGTAAAGGA+AGG 0.539687 8:-81688768 MsG0880047081.01.T01:CDS
CACAACTTTGTAAATTCCAA+TGG 0.553300 8:+81688734 None:intergenic
AGTATAATTGAAGGAGATGG+AGG 0.558565 8:-81689606 MsG0880047081.01.T01:CDS
TTTGTTTCAGATATTTGAAG+GGG 0.583251 8:+81688967 None:intergenic
TGAGGAATACTCATGTGTAA+AGG 0.583283 8:+81689719 None:intergenic
CAAATACACTATGATTGAAG+GGG 0.588174 8:-81688910 MsG0880047081.01.T01:CDS
GAACATACGCGATGGTGCAA+CGG 0.605469 8:+81689695 None:intergenic
CTAACATCTTTAACAAACTG+TGG 0.611198 8:+81689627 None:intergenic
TTTGAGGCTACAAATGATGG+AGG 0.641637 8:-81688843 MsG0880047081.01.T01:CDS
GATTGAAGGGGATCCATTAG+GGG 0.664801 8:-81688898 MsG0880047081.01.T01:CDS
AAAGCTGTGAACATACGCGA+TGG 0.674937 8:+81689687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAAATTTAATAGTTTTTT+TGG + Chr8:81688979-81688998 None:intergenic 10.0%
!!! TAAGTTTTTATAAAAAAAAG+TGG + Chr8:81689144-81689163 None:intergenic 10.0%
!! CTAAGATAAAATTTCTATTT+AGG + Chr8:81689083-81689102 None:intergenic 15.0%
!! AAAGATGTTAGTATAATTGA+AGG - Chr8:81688812-81688831 MsG0880047081.01.T01:CDS 20.0%
!! AATTCTTTAACCAATATTTC+GGG + Chr8:81689060-81689079 None:intergenic 20.0%
!! GAATTCTTTAACCAATATTT+CGG + Chr8:81689061-81689080 None:intergenic 20.0%
!!! AAAATATTTTTGGATTGCTT+CGG - Chr8:81689347-81689366 MsG0880047081.01.T01:intron 20.0%
!!! TTTTGTTTCAGATATTTGAA+GGG + Chr8:81689464-81689483 None:intergenic 20.0%
! ATTCAAGAAATAAACTTCTC+TGG + Chr8:81689404-81689423 None:intergenic 25.0%
! ATTCTTTAACCAATATTTCG+GGG + Chr8:81689059-81689078 None:intergenic 25.0%
! TTTGTTTCAGATATTTGAAG+GGG + Chr8:81689463-81689482 None:intergenic 25.0%
!! GTATTTCTATTATGATATGC+AGG - Chr8:81689434-81689453 MsG0880047081.01.T01:intron 25.0%
!!! CTTTTGTTTCAGATATTTGA+AGG + Chr8:81689465-81689484 None:intergenic 25.0%
AAAGATTGATGTGCTTGATA+AGG - Chr8:81689481-81689500 MsG0880047081.01.T01:intron 30.0%
AAATGTTTAACCAATGTCGT+TGG - Chr8:81689172-81689191 MsG0880047081.01.T01:intron 30.0%
AATGTTTAACCAATGTCGTT+GGG - Chr8:81689173-81689192 MsG0880047081.01.T01:intron 30.0%
CAAATACACTATGATTGAAG+GGG - Chr8:81689517-81689536 MsG0880047081.01.T01:intron 30.0%
CACAACTTTGTAAATTCCAA+TGG + Chr8:81689696-81689715 None:intergenic 30.0%
CATTGGAATTTACAAAGTTG+TGG - Chr8:81689694-81689713 MsG0880047081.01.T01:CDS 30.0%
CTAACATCTTTAACAAACTG+TGG + Chr8:81688803-81688822 None:intergenic 30.0%
GCAAATACACTATGATTGAA+GGG - Chr8:81689516-81689535 MsG0880047081.01.T01:intron 30.0%
GTTAGTATAATTGAAGGAGA+TGG - Chr8:81688818-81688837 MsG0880047081.01.T01:CDS 30.0%
TGCAAATACACTATGATTGA+AGG - Chr8:81689515-81689534 MsG0880047081.01.T01:intron 30.0%
TGTTAAAGAGGAAGATGTAA+AGG - Chr8:81689655-81689674 MsG0880047081.01.T01:CDS 30.0%
!! TGGAGATTTTGATGTTAAAG+AGG - Chr8:81689643-81689662 MsG0880047081.01.T01:CDS 30.0%
AAAGAGGAAGATGTAAAGGA+AGG - Chr8:81689659-81689678 MsG0880047081.01.T01:CDS 35.0%
AGTATAATTGAAGGAGATGG+AGG - Chr8:81688821-81688840 MsG0880047081.01.T01:CDS 35.0%
ATGTTTAACCAATGTCGTTG+GGG - Chr8:81689174-81689193 MsG0880047081.01.T01:intron 35.0%
GAACAAGTCAACTTCAATGA+AGG - Chr8:81688857-81688876 MsG0880047081.01.T01:CDS 35.0%
GCTTGAATCTATTGCTTATG+AGG - Chr8:81689556-81689575 MsG0880047081.01.T01:intron 35.0%
TGAGGAATACTCATGTGTAA+AGG + Chr8:81688711-81688730 None:intergenic 35.0%
TGGAGGTTGTCTATGTAAAA+TGG - Chr8:81689601-81689620 MsG0880047081.01.T01:CDS 35.0%
! AAGTTTGAGGCTACAAATGA+TGG - Chr8:81689581-81689600 MsG0880047081.01.T01:CDS 35.0%
! TCTAAGACCTCAATATGTGT+TGG + Chr8:81688919-81688938 None:intergenic 35.0%
!!! ATATTTTTGGATTGCTTCGG+TGG - Chr8:81689350-81689369 MsG0880047081.01.T01:intron 35.0%
ACAAACTGTGGTAAGAGCTT+TGG + Chr8:81688791-81688810 None:intergenic 40.0%
AGGAAGATGTAAAGGAAGGA+AGG - Chr8:81689663-81689682 MsG0880047081.01.T01:CDS 40.0%
AGTCGATCCAACACATATTG+AGG - Chr8:81688909-81688928 MsG0880047081.01.T01:CDS 40.0%
ATGATTGAAGGGGATCCATT+AGG - Chr8:81689527-81689546 MsG0880047081.01.T01:intron 40.0%
CAAACTGTGGTAAGAGCTTT+GGG + Chr8:81688790-81688809 None:intergenic 40.0%
GCAAGCAGTTACAAGACTAT+TGG - Chr8:81689623-81689642 MsG0880047081.01.T01:CDS 40.0%
GGAAGATGTAAAGGAAGGAA+GGG - Chr8:81689664-81689683 MsG0880047081.01.T01:CDS 40.0%
TAATCACTGCCCCGAAATAT+TGG - Chr8:81689047-81689066 MsG0880047081.01.T01:intron 40.0%
TGATTGAAGGGGATCCATTA+GGG - Chr8:81689528-81689547 MsG0880047081.01.T01:intron 40.0%
TGCTTATGAGGTGAAGTTTG+AGG - Chr8:81689568-81689587 MsG0880047081.01.T01:intron 40.0%
TGTTTAACCAATGTCGTTGG+GGG - Chr8:81689175-81689194 MsG0880047081.01.T01:intron 40.0%
TTTATCTCATGTCGACCCAA+TGG + Chr8:81689381-81689400 None:intergenic 40.0%
! TTTGAGGCTACAAATGATGG+AGG - Chr8:81689584-81689603 MsG0880047081.01.T01:CDS 40.0%
AAAGCTGTGAACATACGCGA+TGG + Chr8:81688743-81688762 None:intergenic 45.0%
AGTTGTGGAGTCATATCTGC+TGG - Chr8:81689709-81689728 MsG0880047081.01.T01:CDS 45.0%
GATTCAAGCTTGTCCCCTAA+TGG + Chr8:81689545-81689564 None:intergenic 45.0%
GATTGAAGGGGATCCATTAG+GGG - Chr8:81689529-81689548 MsG0880047081.01.T01:intron 45.0%
!! GAAGGAAGGGAAAGTACCAT+TGG - Chr8:81689677-81689696 MsG0880047081.01.T01:CDS 45.0%
!! GCTTCGGTGGTTTTACCATT+GGG - Chr8:81689363-81689382 MsG0880047081.01.T01:intron 45.0%
!! TGCTTCGGTGGTTTTACCAT+TGG - Chr8:81689362-81689381 MsG0880047081.01.T01:intron 45.0%
!!! AAATATTGATAAAATATTTT+TGG - Chr8:81689337-81689356 MsG0880047081.01.T01:intron 5.0%
AAAGAGTCCCCCAACGACAT+TGG + Chr8:81689185-81689204 None:intergenic 50.0%
ATTGAAGGAGATGGAGGAGC+TGG - Chr8:81688827-81688846 MsG0880047081.01.T01:CDS 50.0%
GAACATACGCGATGGTGCAA+CGG + Chr8:81688735-81688754 None:intergenic 50.0%
ACGCGATGGTGCAACGGTTG+AGG + Chr8:81688729-81688748 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 81688695 81689754 81688695 ID=MsG0880047081.01;Name=MsG0880047081.01
Chr8 mRNA 81688695 81689754 81688695 ID=MsG0880047081.01.T01;Parent=MsG0880047081.01;Name=MsG0880047081.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|160
Chr8 exon 81689571 81689754 81689571 ID=MsG0880047081.01.T01:exon:35161;Parent=MsG0880047081.01.T01
Chr8 exon 81688695 81688993 81688695 ID=MsG0880047081.01.T01:exon:35160;Parent=MsG0880047081.01.T01
Chr8 CDS 81689571 81689754 81689571 ID=MsG0880047081.01.T01:cds;Parent=MsG0880047081.01.T01
Chr8 CDS 81688695 81688993 81688695 ID=MsG0880047081.01.T01:cds;Parent=MsG0880047081.01.T01
Gene Sequence

>MsG0880047081.01.T01

ATGGGAGTAACAACCTTTACACATGAGTATTCCTCAACCGTTGCACCATCGCGTATGTTCACAGCTTTGATCATAGACTCTAGAAATTTGATCCCAAAGCTCTTACCACAGTTTGTTAAAGATGTTAGTATAATTGAAGGAGATGGAGGAGCTGGAAGCATTGAACAAGTCAACTTCAATGAAGGTAGCCCCTTCAAATATCTGAAACAAAAGATTGATGTGCTTGATAAGGAAAATTTGATTTGCAAATACACTATGATTGAAGGGGATCCATTAGGGGACAAGCTTGAATCTATTGCTTATGAGGTGAAGTTTGAGGCTACAAATGATGGAGGTTGTCTATGTAAAATGGCAAGCAGTTACAAGACTATTGGAGATTTTGATGTTAAAGAGGAAGATGTAAAGGAAGGAAGGGAAAGTACCATTGGAATTTACAAAGTTGTGGAGTCATATCTGCTGGAGAATCCACAAGTCTATGCTTGA

Protein sequence

>MsG0880047081.01.T01

MGVTTFTHEYSSTVAPSRMFTALIIDSRNLIPKLLPQFVKDVSIIEGDGGAGSIEQVNFNEGSPFKYLKQKIDVLDKENLICKYTMIEGDPLGDKLESIAYEVKFEATNDGGCLCKMASSYKTIGDFDVKEEDVKEGRESTIGIYKVVESYLLENPQVYA*