AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038985.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038985.01.T01 MTR_7g066110 92.308 169 13 0 11 179 1 169 2.89e-116 327
MsG0780038985.01.T01 MTR_4g018860 71.598 169 48 0 1 169 1 169 1.33e-87 256
MsG0780038985.01.T01 MTR_4g018880 68.539 178 56 0 1 178 1 178 5.48e-84 246
MsG0780038985.01.T01 MTR_4g018850 68.508 181 55 1 1 179 1 181 2.42e-79 235
MsG0780038985.01.T01 MTR_4g018880 70.455 132 39 0 1 132 1 132 3.03e-62 189
MsG0780038985.01.T01 MTR_6g452730 47.953 171 85 2 7 176 4 171 2.82e-54 171
MsG0780038985.01.T01 MTR_1g101120 51.923 156 74 1 12 166 15 170 2.88e-54 171
MsG0780038985.01.T01 MTR_7g066090 81.579 76 14 0 1 76 1 76 1.37e-38 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038985.01.T01 AT3G54040 57.778 180 70 4 1 178 1 176 4.11e-68 206
MsG0780038985.01.T01 AT3G54040 60.355 169 61 4 1 167 57 221 1.89e-67 206
MsG0780038985.01.T01 AT5G52390 45.251 179 97 1 1 178 1 179 1.34e-52 166

Find 45 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAATATTGGGGCAATTT+TGG 0.141377 7:+56416363 None:intergenic
TGTTGACCTTTACTTCAATT+TGG 0.213939 7:-56416345 MsG0780038985.01.T01:CDS
GAGCATAATCAAAGGTAATT+TGG 0.227054 7:+56416802 None:intergenic
CGAAGGATGCATGGATAAAA+AGG 0.255435 7:+56416771 None:intergenic
GAAGTAAAGGTCAACAATAT+TGG 0.286026 7:+56416352 None:intergenic
AAGTAAAGGTCAACAATATT+GGG 0.297379 7:+56416353 None:intergenic
CTTTACTTCAATTTGGCTGC+TGG 0.332953 7:-56416338 MsG0780038985.01.T01:CDS
TCTTTCTCAATAGCTTCTTC+AGG 0.363584 7:-56416599 MsG0780038985.01.T01:CDS
GTGAGCTCTGGAGCTGCACC+TGG 0.378232 7:-56415725 MsG0780038985.01.T01:CDS
CTTAGGATACTTTGTTTACA+TGG 0.379493 7:+56415652 None:intergenic
TCTTGTGACAGAGCAGAAAC+AGG 0.399775 7:+56415701 None:intergenic
TTCAGGAAAACGGTGTTTGT+TGG 0.405418 7:-56416582 MsG0780038985.01.T01:CDS
ATGATCGAGCTTGTGAGCTC+TGG 0.439225 7:-56415737 MsG0780038985.01.T01:CDS
TTCAATTTGGCTGCTGGGGA+AGG 0.450339 7:-56416332 MsG0780038985.01.T01:intron
TTTACTTCAATTTGGCTGCT+GGG 0.473999 7:-56416337 MsG0780038985.01.T01:CDS
CAATAGCTTCTTCAGGAAAA+CGG 0.477557 7:-56416592 MsG0780038985.01.T01:CDS
AGAAGTAGTGGTGGAGCAAA+TGG 0.478800 7:-56416510 MsG0780038985.01.T01:CDS
TACTTTGTTTACATGGGAGA+AGG 0.498445 7:+56415659 None:intergenic
CAGCAGCCAAATTGAAGTAA+AGG 0.504408 7:+56416339 None:intergenic
GACAGAGCAGAAACAGGGCC+AGG 0.529854 7:+56415707 None:intergenic
TTAGGATACTTTGTTTACAT+GGG 0.551833 7:+56415653 None:intergenic
ACTTTGTTTACATGGGAGAA+GGG 0.558007 7:+56415660 None:intergenic
GCTCGATCATAGCGCGATGA+GGG 0.572009 7:+56415750 None:intergenic
CTTTGTTTACATGGGAGAAG+GGG 0.572016 7:+56415661 None:intergenic
ACAGAGAAGGCAGCGGATGG+TGG 0.581266 7:-56416557 MsG0780038985.01.T01:CDS
GTAGTGGTGGAGCAAATGGC+GGG 0.589388 7:-56416506 MsG0780038985.01.T01:CDS
GTGTTTGTTGGAGACAGAGA+AGG 0.593902 7:-56416570 MsG0780038985.01.T01:CDS
TTACTTCAATTTGGCTGCTG+GGG 0.597417 7:-56416336 MsG0780038985.01.T01:CDS
GATCATAGCGCGATGAGGGT+CGG 0.606329 7:+56415754 None:intergenic
ATGTAGGACATCAGAAGTAG+TGG 0.608155 7:-56416522 MsG0780038985.01.T01:CDS
TGTGTGTACCGAAGGATGCA+TGG 0.608900 7:+56416762 None:intergenic
CTACTTCTGATGTCCTACAT+TGG 0.613644 7:+56416525 None:intergenic
GAGACAGAGAAGGCAGCGGA+TGG 0.613801 7:-56416560 MsG0780038985.01.T01:CDS
AGCTCGATCATAGCGCGATG+AGG 0.614616 7:+56415749 None:intergenic
AGTAGTGGTGGAGCAAATGG+CGG 0.620474 7:-56416507 MsG0780038985.01.T01:CDS
TGTGTGTGAGGAGTTGCAAA+AGG 0.620931 7:-56416630 MsG0780038985.01.T01:CDS
GTTGGAGACAGAGAAGGCAG+CGG 0.628566 7:-56416564 MsG0780038985.01.T01:CDS
GAACACAAGAGCATAATCAA+AGG 0.628881 7:+56416794 None:intergenic
TAGGACATCAGAAGTAGTGG+TGG 0.629632 7:-56416519 MsG0780038985.01.T01:CDS
CTTGTGACAGAGCAGAAACA+GGG 0.634654 7:+56415702 None:intergenic
AGTAAAGGTCAACAATATTG+GGG 0.636729 7:+56416354 None:intergenic
GTGGCGTCGAGTACCAATGT+AGG 0.640711 7:-56416538 MsG0780038985.01.T01:CDS
GATCAATGTGTGAATGCTTG+CGG 0.665620 7:-56416470 MsG0780038985.01.T01:CDS
GACAGCTGAAGTTGTGTGTG+AGG 0.677896 7:-56416642 MsG0780038985.01.T01:intron
TGAATGCTTGCGGAGTTGAG+AGG 0.714850 7:-56416460 MsG0780038985.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATAAATAAATTGCAT+AGG + Chr7:56415791-56415810 None:intergenic 10.0%
!! AACAAAAAATAAAACAAAGA+AGG - Chr7:56416569-56416588 MsG0780038985.01.T01:CDS 15.0%
!! TAACTTCAAATTTAATCAAA+GGG - Chr7:56416524-56416543 MsG0780038985.01.T01:CDS 15.0%
!! TTAACTTCAAATTTAATCAA+AGG - Chr7:56416523-56416542 MsG0780038985.01.T01:CDS 15.0%
!! TTTGAACAATTCAAATAATT+GGG - Chr7:56416267-56416286 MsG0780038985.01.T01:intron 15.0%
!!! TTTTGAACAATTCAAATAAT+TGG - Chr7:56416266-56416285 MsG0780038985.01.T01:intron 15.0%
!! AAGTAATTAAGACAAAAAGA+AGG + Chr7:56416365-56416384 None:intergenic 20.0%
!! CTCTCTTGATATTATAATAA+AGG - Chr7:56416644-56416663 MsG0780038985.01.T01:intron 20.0%
!! TAATTGGAAAATATATAGCA+GGG + Chr7:56415757-56415776 None:intergenic 20.0%
!!! TTCTTTTTGTCTTAATTACT+TGG - Chr7:56416364-56416383 MsG0780038985.01.T01:CDS 20.0%
! AAGTAAAGGTCAACAATATT+GGG + Chr7:56416132-56416151 None:intergenic 25.0%
! ATAGGTAACTAGATAGTAAT+TGG + Chr7:56415773-56415792 None:intergenic 25.0%
! ATCTTCATGCATAATAGTTA+TGG + Chr7:56416197-56416216 None:intergenic 25.0%
! CTGTTCAAAATAAGATGAAA+AGG + Chr7:56416332-56416351 None:intergenic 25.0%
! GTAATTGGAAAATATATAGC+AGG + Chr7:56415758-56415777 None:intergenic 25.0%
! GTTAAAATGTTAGATCATGA+CGG + Chr7:56416508-56416527 None:intergenic 25.0%
!! TTTTTGTCTTAATTACTTGG+TGG - Chr7:56416367-56416386 MsG0780038985.01.T01:CDS 25.0%
ACTATTATGCATGAAGATCA+TGG - Chr7:56416199-56416218 MsG0780038985.01.T01:intron 30.0%
AGTAAAGGTCAACAATATTG+GGG + Chr7:56416131-56416150 None:intergenic 30.0%
GAAGTAAAGGTCAACAATAT+TGG + Chr7:56416133-56416152 None:intergenic 30.0%
GAGCATAATCAAAGGTAATT+TGG + Chr7:56415683-56415702 None:intergenic 30.0%
TGTTGACCTTTACTTCAATT+TGG - Chr7:56416137-56416156 MsG0780038985.01.T01:intron 30.0%
AATTGGGTCATAGTGATTTG+TGG - Chr7:56416283-56416302 MsG0780038985.01.T01:intron 35.0%
CAATAGCTTCTTCAGGAAAA+CGG - Chr7:56415890-56415909 MsG0780038985.01.T01:intron 35.0%
CTTTGATACTATGCAAATGC+AGG - Chr7:56416670-56416689 MsG0780038985.01.T01:intron 35.0%
GAAGGATGCATGGATAAAAA+GGG + Chr7:56415713-56415732 None:intergenic 35.0%
TCTTTCTCAATAGCTTCTTC+AGG - Chr7:56415883-56415902 MsG0780038985.01.T01:intron 35.0%
TTTACTTCAATTTGGCTGCT+GGG - Chr7:56416145-56416164 MsG0780038985.01.T01:intron 35.0%
! CAACAATATTGGGGCAATTT+TGG + Chr7:56416122-56416141 None:intergenic 35.0%
! CTTTTTATCCATGCATCCTT+CGG - Chr7:56415712-56415731 MsG0780038985.01.T01:CDS 35.0%
! GAACACAAGAGCATAATCAA+AGG + Chr7:56415691-56415710 None:intergenic 35.0%
! GAAGGTTCTTTTCTTGTAGA+AGG - Chr7:56416587-56416606 MsG0780038985.01.T01:CDS 35.0%
!! CAAAGTTTTGCTGTGAATTG+TGG + Chr7:56416084-56416103 None:intergenic 35.0%
ATGTAGGACATCAGAAGTAG+TGG - Chr7:56415960-56415979 MsG0780038985.01.T01:intron 40.0%
CAGCAGCCAAATTGAAGTAA+AGG + Chr7:56416146-56416165 None:intergenic 40.0%
CGAAGGATGCATGGATAAAA+AGG + Chr7:56415714-56415733 None:intergenic 40.0%
CTACTTCTGATGTCCTACAT+TGG + Chr7:56415960-56415979 None:intergenic 40.0%
CTTTACTTCAATTTGGCTGC+TGG - Chr7:56416144-56416163 MsG0780038985.01.T01:intron 40.0%
GATCAATGTGTGAATGCTTG+CGG - Chr7:56416012-56416031 MsG0780038985.01.T01:intron 40.0%
GTATATAGTGTGTGTACCGA+AGG + Chr7:56415731-56415750 None:intergenic 40.0%
TTACTTCAATTTGGCTGCTG+GGG - Chr7:56416146-56416165 MsG0780038985.01.T01:intron 40.0%
TTCAGGAAAACGGTGTTTGT+TGG - Chr7:56415900-56415919 MsG0780038985.01.T01:intron 40.0%
! GGAGTTGAGAGGAATTTTGT+TGG - Chr7:56416033-56416052 MsG0780038985.01.T01:intron 40.0%
! TTTTGCATCTCACAAAGGTC+AGG + Chr7:56416706-56416725 None:intergenic 40.0%
CTTGTGACAGAGCAGAAACA+GGG + Chr7:56416783-56416802 None:intergenic 45.0%
TAGGACATCAGAAGTAGTGG+TGG - Chr7:56415963-56415982 MsG0780038985.01.T01:intron 45.0%
TCTTGTGACAGAGCAGAAAC+AGG + Chr7:56416784-56416803 None:intergenic 45.0%
TGTGTGTGAGGAGTTGCAAA+AGG - Chr7:56415852-56415871 MsG0780038985.01.T01:intron 45.0%
! AGAAGTAGTGGTGGAGCAAA+TGG - Chr7:56415972-56415991 MsG0780038985.01.T01:intron 45.0%
! CGGTCTTTTGCATCTCACAA+AGG + Chr7:56416711-56416730 None:intergenic 45.0%
! GTGTTTGTTGGAGACAGAGA+AGG - Chr7:56415912-56415931 MsG0780038985.01.T01:intron 45.0%
ATGATCGAGCTTGTGAGCTC+TGG - Chr7:56416745-56416764 MsG0780038985.01.T01:intron 50.0%
GACAGCTGAAGTTGTGTGTG+AGG - Chr7:56415840-56415859 MsG0780038985.01.T01:intron 50.0%
TGAATGCTTGCGGAGTTGAG+AGG - Chr7:56416022-56416041 MsG0780038985.01.T01:intron 50.0%
TGTGTGTACCGAAGGATGCA+TGG + Chr7:56415723-56415742 None:intergenic 50.0%
TTCAATTTGGCTGCTGGGGA+AGG - Chr7:56416150-56416169 MsG0780038985.01.T01:intron 50.0%
! AGTAGTGGTGGAGCAAATGG+CGG - Chr7:56415975-56415994 MsG0780038985.01.T01:intron 50.0%
AGCTCGATCATAGCGCGATG+AGG + Chr7:56416736-56416755 None:intergenic 55.0%
GATCATAGCGCGATGAGGGT+CGG + Chr7:56416731-56416750 None:intergenic 55.0%
GCTCGATCATAGCGCGATGA+GGG + Chr7:56416735-56416754 None:intergenic 55.0%
GTTGGAGACAGAGAAGGCAG+CGG - Chr7:56415918-56415937 MsG0780038985.01.T01:intron 55.0%
! GTAGTGGTGGAGCAAATGGC+GGG - Chr7:56415976-56415995 MsG0780038985.01.T01:intron 55.0%
! GTGGCGTCGAGTACCAATGT+AGG - Chr7:56415944-56415963 MsG0780038985.01.T01:intron 55.0%
ACAGAGAAGGCAGCGGATGG+TGG - Chr7:56415925-56415944 MsG0780038985.01.T01:intron 60.0%
GACAGAGCAGAAACAGGGCC+AGG + Chr7:56416778-56416797 None:intergenic 60.0%
GAGACAGAGAAGGCAGCGGA+TGG - Chr7:56415922-56415941 MsG0780038985.01.T01:intron 60.0%
!! GTGAGCTCTGGAGCTGCACC+TGG - Chr7:56416757-56416776 MsG0780038985.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 56415667 56416837 56415667 ID=MsG0780038985.01;Name=MsG0780038985.01
Chr7 mRNA 56415667 56416837 56415667 ID=MsG0780038985.01.T01;Parent=MsG0780038985.01;Name=MsG0780038985.01.T01;_AED=0.34;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|179
Chr7 exon 56416771 56416837 56416771 ID=MsG0780038985.01.T01:exon:6397;Parent=MsG0780038985.01.T01
Chr7 exon 56416333 56416659 56416333 ID=MsG0780038985.01.T01:exon:6396;Parent=MsG0780038985.01.T01
Chr7 exon 56415667 56415812 56415667 ID=MsG0780038985.01.T01:exon:6395;Parent=MsG0780038985.01.T01
Chr7 CDS 56416771 56416837 56416771 ID=MsG0780038985.01.T01:cds;Parent=MsG0780038985.01.T01
Chr7 CDS 56416333 56416659 56416333 ID=MsG0780038985.01.T01:cds;Parent=MsG0780038985.01.T01
Chr7 CDS 56415667 56415812 56415667 ID=MsG0780038985.01.T01:cds;Parent=MsG0780038985.01.T01
Gene Sequence

>MsG0780038985.01.T01

ATGTCTTTCTCATCCAAATTACCTTTGATTATGCTCTTGTGTTCCCTTTTTATCCATGCATCCTTCGCTGAAGTTGTGTGTGAGGAGTTGCAAAAGGATCTATGTTCTTTCTCAATAGCTTCTTCAGGAAAACGGTGTTTGTTGGAGACAGAGAAGGCAGCGGATGGTGGCGTCGAGTACCAATGTAGGACATCAGAAGTAGTGGTGGAGCAAATGGCGGGATACATAGAGACAGATCAATGTGTGAATGCTTGCGGAGTTGAGAGGAATTTTGTTGGAATTTCTTCTGATGCATTGCTTGAGCCACAATTCACAGCAAAACTTTGTTCTTCGTCTTGTTACCAAAATTGCCCCAATATTGTTGACCTTTACTTCAATTTGGCTGCTGGGGAAGGTGTATTTTTGCCTGACCTTTGTGAGATGCAAAAGACCGACCCTCATCGCGCTATGATCGAGCTTGTGAGCTCTGGAGCTGCACCTGGCCCTGTTTCTGCTCTGTCACAAGACAAGCATGCAGCATTTGCCCCTTCTCCCATGTAA

Protein sequence

>MsG0780038985.01.T01

MSFSSKLPLIMLLCSLFIHASFAEVVCEELQKDLCSFSIASSGKRCLLETEKAADGGVEYQCRTSEVVVEQMAGYIETDQCVNACGVERNFVGISSDALLEPQFTAKLCSSSCYQNCPNIVDLYFNLAAGEGVFLPDLCEMQKTDPHRAMIELVSSGAAPGPVSALSQDKHAAFAPSPM*