AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041637.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041637.01.T01 MTR_7g114900 100.000 213 0 0 1 213 68 280 5.64e-154 428
MsG0780041637.01.T01 MTR_1g108440 66.822 214 70 1 1 213 55 268 1.40e-98 287
MsG0780041637.01.T01 MTR_2g032540 27.556 225 148 5 1 213 67 288 7.80e-24 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041637.01.T01 AT3G04790 78.404 213 46 0 1 213 64 276 6.34e-121 344
MsG0780041637.01.T01 AT2G01290 61.972 213 81 0 1 213 52 264 1.11e-92 272
MsG0780041637.01.T01 AT1G71100 60.550 218 81 1 1 213 48 265 1.38e-86 257
MsG0780041637.01.T01 AT5G44520 31.532 222 136 5 1 209 56 274 1.39e-25 101
MsG0780041637.01.T01 AT5G44520 31.532 222 136 5 1 209 86 304 2.54e-25 100

Find 61 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTTTCTCAATCTTGTTAA+AGG 0.131907 7:-93327584 MsG0780041637.01.T01:CDS
GTTGAGAAGTTCGCCGAGTT+TGG 0.275002 7:+93327748 None:intergenic
TGGTATTCCTCTCTCTGTTC+TGG 0.303086 7:-93327657 MsG0780041637.01.T01:CDS
TGCTCTCCTCCGTGAGAAAA+TGG 0.332089 7:-93327552 MsG0780041637.01.T01:CDS
GTTGGAAATATAATTTGATT+AGG 0.342353 7:-93327424 MsG0780041637.01.T01:CDS
GTCATCATTGCTGGTAAAAC+TGG 0.352685 7:-93327197 MsG0780041637.01.T01:CDS
TGATAAATTCGTTGTGGTCG+TGG 0.378141 7:-93327516 MsG0780041637.01.T01:CDS
AGCCTGTTCTTCGGTGCGTT+TGG 0.391354 7:+93327688 None:intergenic
AGCGGCATGGTCCTCGGCCT+CGG 0.399773 7:-93327800 None:intergenic
TTCCAAACGCACCGAAGAAC+AGG 0.400283 7:-93327690 MsG0780041637.01.T01:CDS
TTCTTCGGTGCGTTTGGAAG+TGG 0.409826 7:+93327694 None:intergenic
GCTACCTCCGTCATCATTGC+TGG 0.417733 7:-93327206 MsG0780041637.01.T01:CDS
GCTGAGATTTCGTCGCTTGA+AGG 0.433006 7:-93327260 MsG0780041637.01.T01:CDS
TTAGAATTATCAGTAACATA+TGG 0.433220 7:+93327338 None:intergenic
GACGAAGGCGGCGGTGGAGC+CGG 0.434905 7:+93327775 None:intergenic
CAAGAATTGTTCAAAGAAGA+AGG 0.437979 7:-93327398 MsG0780041637.01.T01:CDS
GAAGAACAGGCTCGTTCTCT+TGG 0.445449 7:-93327677 MsG0780041637.01.T01:CDS
CTCAATCTTGTTAAAGGCCG+CGG 0.449503 7:-93327578 MsG0780041637.01.T01:CDS
CGGTCTCGGTGGAAGCGGTT+TGG 0.459657 7:-93327474 MsG0780041637.01.T01:CDS
TGGAAGCGGTTTGGCGATGC+CGG 0.461565 7:-93327465 MsG0780041637.01.T01:CDS
CGGTTTGGCGATGCCGGTGG+AGG 0.471807 7:-93327459 MsG0780041637.01.T01:CDS
GTCTCCGGTCTCGGTGGAAG+CGG 0.476533 7:-93327479 MsG0780041637.01.T01:CDS
TGGAGCCGGTGCCGAGGCCG+AGG 0.476978 7:+93327789 None:intergenic
GGAGACGACGAAGGCGGCGG+TGG 0.479468 7:+93327769 None:intergenic
ATCAGAGATGCAAATGCTGC+TGG 0.488042 7:-93327284 MsG0780041637.01.T01:CDS
ATGGTCCTCGGCCTCGGCAC+CGG 0.489708 7:-93327794 MsG0780041637.01.T01:CDS
ACAAAACTCGTCTCCGGTCT+CGG 0.492994 7:-93327488 MsG0780041637.01.T01:CDS
GTCAAAAGCGGCATGGTCCT+CGG 0.496677 7:-93327806 None:intergenic
AGCAGCATTTGCATCTCTGA+TGG 0.500428 7:+93327286 None:intergenic
CTTGAAGGTGTCGTCGAGCA+TGG 0.504176 7:-93327245 MsG0780041637.01.T01:CDS
TCTTCGGTGCGTTTGGAAGT+GGG 0.509994 7:+93327695 None:intergenic
GCATGGTTTGTTCTTGAACA+TGG 0.523331 7:-93327228 MsG0780041637.01.T01:CDS
CGTCTCGATCTCGCCATCGA+CGG 0.527285 7:-93327623 MsG0780041637.01.T01:CDS
AAGAGAACGAGCCTGTTCTT+CGG 0.529963 7:+93327679 None:intergenic
CGGCGGTGGAGCCGGTGCCG+AGG 0.537408 7:+93327783 None:intergenic
CAAACCGCTTCCACCGAGAC+CGG 0.537805 7:+93327475 None:intergenic
GATGATGTTGGTGAGTTCGC+CGG 0.539899 7:+93327724 None:intergenic
GCCTTCGTCGTCTCCAAACT+CGG 0.546205 7:-93327761 MsG0780041637.01.T01:CDS
GACGACACAAAACTCGTCTC+CGG 0.548040 7:-93327494 MsG0780041637.01.T01:CDS
AAGCGGTTTGGCGATGCCGG+TGG 0.557548 7:-93327462 MsG0780041637.01.T01:CDS
GGCGAACTCACCAACATCAT+CGG 0.560538 7:-93327722 MsG0780041637.01.T01:CDS
CAAAACTGAACAACCTCCAC+CGG 0.562915 7:+93327446 None:intergenic
TTTGGAGACGACGAAGGCGG+CGG 0.566766 7:+93327766 None:intergenic
CACAACGAATTTATCAGAAG+CGG 0.572098 7:+93327523 None:intergenic
GCAGCATTTGCATCTCTGAT+GGG 0.580699 7:+93327287 None:intergenic
CCTTTAACAAGATTGAGAAA+CGG 0.589530 7:+93327584 None:intergenic
CGCTTCTGATAAATTCGTTG+TGG 0.592344 7:-93327522 MsG0780041637.01.T01:CDS
TTATCGTCCAGAACAGAGAG+AGG 0.606235 7:+93327650 None:intergenic
GACTTCATCAGCACCGTCGA+TGG 0.609689 7:+93327610 None:intergenic
AAACTCGTCTCCGGTCTCGG+TGG 0.623284 7:-93327485 MsG0780041637.01.T01:CDS
AGTGGGAACGCCGATGATGT+TGG 0.637767 7:+93327712 None:intergenic
TGTTGATGCGAAATTGAGAG+TGG 0.639035 7:-93327375 MsG0780041637.01.T01:CDS
GCCGAGTTTGGAGACGACGA+AGG 0.645857 7:+93327760 None:intergenic
AAATTGAGAGTGGATGAAAG+TGG 0.649838 7:-93327365 MsG0780041637.01.T01:CDS
GAGTTTGGAGACGACGAAGG+CGG 0.656291 7:+93327763 None:intergenic
CTCGGCGAACTTCTCAACTC+CGG 0.656946 7:-93327743 MsG0780041637.01.T01:CDS
CACGGAGGAGAGCACCGCCG+CGG 0.668975 7:+93327561 None:intergenic
TCTCCTCCGTGAGAAAATGG+TGG 0.693512 7:-93327549 MsG0780041637.01.T01:CDS
TCGATGGCGAGATCGAGACG+AGG 0.700409 7:+93327626 None:intergenic
AATCTTGTTAAAGGCCGCGG+CGG 0.704955 7:-93327575 MsG0780041637.01.T01:CDS
TTTACCAGCAATGATGACGG+AGG 0.706916 7:+93327202 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTGGAAATATAATTTGATT+AGG - Chr7:93327545-93327564 MsG0780041637.01.T01:CDS 20.0%
!! TTAGAATTATCAGTAACATA+TGG + Chr7:93327634-93327653 None:intergenic 20.0%
CAAGAATTGTTCAAAGAAGA+AGG - Chr7:93327571-93327590 MsG0780041637.01.T01:CDS 30.0%
CCTTTAACAAGATTGAGAAA+CGG + Chr7:93327388-93327407 None:intergenic 30.0%
AAATTGAGAGTGGATGAAAG+TGG - Chr7:93327604-93327623 MsG0780041637.01.T01:CDS 35.0%
CACAACGAATTTATCAGAAG+CGG + Chr7:93327449-93327468 None:intergenic 35.0%
CCGTTTCTCAATCTTGTTAA+AGG - Chr7:93327385-93327404 MsG0780041637.01.T01:CDS 35.0%
! AGTTTTACCAGCAATGATGA+CGG + Chr7:93327773-93327792 None:intergenic 35.0%
CGCTTCTGATAAATTCGTTG+TGG - Chr7:93327447-93327466 MsG0780041637.01.T01:CDS 40.0%
GTCATCATTGCTGGTAAAAC+TGG - Chr7:93327772-93327791 MsG0780041637.01.T01:CDS 40.0%
TGATAAATTCGTTGTGGTCG+TGG - Chr7:93327453-93327472 MsG0780041637.01.T01:CDS 40.0%
TGTTGATGCGAAATTGAGAG+TGG - Chr7:93327594-93327613 MsG0780041637.01.T01:CDS 40.0%
!! GCATGGTTTGTTCTTGAACA+TGG - Chr7:93327741-93327760 MsG0780041637.01.T01:CDS 40.0%
!! TGGAGGTTGTTCAGTTTTGT+TGG - Chr7:93327527-93327546 MsG0780041637.01.T01:CDS 40.0%
AAGAGAACGAGCCTGTTCTT+CGG + Chr7:93327293-93327312 None:intergenic 45.0%
AGCAGCATTTGCATCTCTGA+TGG + Chr7:93327686-93327705 None:intergenic 45.0%
ATCAGAGATGCAAATGCTGC+TGG - Chr7:93327685-93327704 MsG0780041637.01.T01:CDS 45.0%
CAAAACTGAACAACCTCCAC+CGG + Chr7:93327526-93327545 None:intergenic 45.0%
CTCAATCTTGTTAAAGGCCG+CGG - Chr7:93327391-93327410 MsG0780041637.01.T01:CDS 45.0%
GCAGCATTTGCATCTCTGAT+GGG + Chr7:93327685-93327704 None:intergenic 45.0%
TGGTATTCCTCTCTCTGTTC+TGG - Chr7:93327312-93327331 MsG0780041637.01.T01:CDS 45.0%
TTATCGTCCAGAACAGAGAG+AGG + Chr7:93327322-93327341 None:intergenic 45.0%
!! TTTACCAGCAATGATGACGG+AGG + Chr7:93327770-93327789 None:intergenic 45.0%
AATCTTGTTAAAGGCCGCGG+CGG - Chr7:93327394-93327413 MsG0780041637.01.T01:CDS 50.0%
ACAAAACTCGTCTCCGGTCT+CGG - Chr7:93327481-93327500 MsG0780041637.01.T01:CDS 50.0%
GAAGAACAGGCTCGTTCTCT+TGG - Chr7:93327292-93327311 MsG0780041637.01.T01:CDS 50.0%
GACGACACAAAACTCGTCTC+CGG - Chr7:93327475-93327494 MsG0780041637.01.T01:CDS 50.0%
GCTGAGATTTCGTCGCTTGA+AGG - Chr7:93327709-93327728 MsG0780041637.01.T01:CDS 50.0%
GGCGAACTCACCAACATCAT+CGG - Chr7:93327247-93327266 MsG0780041637.01.T01:CDS 50.0%
GTTGAGAAGTTCGCCGAGTT+TGG + Chr7:93327224-93327243 None:intergenic 50.0%
TCTCCTCCGTGAGAAAATGG+TGG - Chr7:93327420-93327439 MsG0780041637.01.T01:CDS 50.0%
TGCTCTCCTCCGTGAGAAAA+TGG - Chr7:93327417-93327436 MsG0780041637.01.T01:CDS 50.0%
TTCCAAACGCACCGAAGAAC+AGG - Chr7:93327279-93327298 MsG0780041637.01.T01:CDS 50.0%
! CGGCTTCCACCATTTTCTCA+CGG + Chr7:93327429-93327448 None:intergenic 50.0%
! CTTCCACCATTTTCTCACGG+AGG + Chr7:93327426-93327445 None:intergenic 50.0%
! GATGATGTTGGTGAGTTCGC+CGG + Chr7:93327248-93327267 None:intergenic 50.0%
! TCTTCGGTGCGTTTGGAAGT+GGG + Chr7:93327277-93327296 None:intergenic 50.0%
! TTCTTCGGTGCGTTTGGAAG+TGG + Chr7:93327278-93327297 None:intergenic 50.0%
AGTGGGAACGCCGATGATGT+TGG + Chr7:93327260-93327279 None:intergenic 55.0%
CTCGGCGAACTTCTCAACTC+CGG - Chr7:93327226-93327245 MsG0780041637.01.T01:CDS 55.0%
GACTTCATCAGCACCGTCGA+TGG + Chr7:93327362-93327381 None:intergenic 55.0%
GAGTTTGGAGACGACGAAGG+CGG + Chr7:93327209-93327228 None:intergenic 55.0%
GCCTTCGTCGTCTCCAAACT+CGG - Chr7:93327208-93327227 MsG0780041637.01.T01:CDS 55.0%
GCTACCTCCGTCATCATTGC+TGG - Chr7:93327763-93327782 MsG0780041637.01.T01:CDS 55.0%
! AGCCTGTTCTTCGGTGCGTT+TGG + Chr7:93327284-93327303 None:intergenic 55.0%
!! CTTGAAGGTGTCGTCGAGCA+TGG - Chr7:93327724-93327743 MsG0780041637.01.T01:CDS 55.0%
AAACTCGTCTCCGGTCTCGG+TGG - Chr7:93327484-93327503 MsG0780041637.01.T01:CDS 60.0%
CAAACCGCTTCCACCGAGAC+CGG + Chr7:93327497-93327516 None:intergenic 60.0%
CGTCTCGATCTCGCCATCGA+CGG - Chr7:93327346-93327365 MsG0780041637.01.T01:CDS 60.0%
GCCGAGTTTGGAGACGACGA+AGG + Chr7:93327212-93327231 None:intergenic 60.0%
TCGATGGCGAGATCGAGACG+AGG + Chr7:93327346-93327365 None:intergenic 60.0%
TTTGGAGACGACGAAGGCGG+CGG + Chr7:93327206-93327225 None:intergenic 60.0%
! TGGAAGCGGTTTGGCGATGC+CGG - Chr7:93327504-93327523 MsG0780041637.01.T01:CDS 60.0%
CGGTCTCGGTGGAAGCGGTT+TGG - Chr7:93327495-93327514 MsG0780041637.01.T01:CDS 65.0%
GTCTCCGGTCTCGGTGGAAG+CGG - Chr7:93327490-93327509 MsG0780041637.01.T01:CDS 65.0%
! AAGCGGTTTGGCGATGCCGG+TGG - Chr7:93327507-93327526 MsG0780041637.01.T01:CDS 65.0%
ATGGTCCTCGGCCTCGGCAC+CGG - Chr7:93327175-93327194 MsG0780041637.01.T01:CDS 70.0%
! CGGTTTGGCGATGCCGGTGG+AGG - Chr7:93327510-93327529 MsG0780041637.01.T01:CDS 70.0%
GACGAAGGCGGCGGTGGAGC+CGG + Chr7:93327197-93327216 None:intergenic 75.0%
GGAGACGACGAAGGCGGCGG+TGG + Chr7:93327203-93327222 None:intergenic 75.0%
!! CACGGAGGAGAGCACCGCCG+CGG + Chr7:93327411-93327430 None:intergenic 75.0%
!! TGGAGCCGGTGCCGAGGCCG+AGG + Chr7:93327183-93327202 None:intergenic 80.0%
!! CGGCGGTGGAGCCGGTGCCG+AGG + Chr7:93327189-93327208 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr7 gene 93327175 93327816 93327175 ID=MsG0780041637.01;Name=MsG0780041637.01
Chr7 mRNA 93327175 93327816 93327175 ID=MsG0780041637.01.T01;Parent=MsG0780041637.01;Name=MsG0780041637.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|213
Chr7 exon 93327175 93327816 93327175 ID=MsG0780041637.01.T01:exon:22089;Parent=MsG0780041637.01.T01
Chr7 CDS 93327175 93327816 93327175 ID=MsG0780041637.01.T01:cds;Parent=MsG0780041637.01.T01
Gene Sequence

>MsG0780041637.01.T01

ATGGTCCTCGGCCTCGGCACCGGCTCCACCGCCGCCTTCGTCGTCTCCAAACTCGGCGAACTTCTCAACTCCGGCGAACTCACCAACATCATCGGCGTTCCCACTTCCAAACGCACCGAAGAACAGGCTCGTTCTCTTGGTATTCCTCTCTCTGTTCTGGACGATAATCCTCGTCTCGATCTCGCCATCGACGGTGCTGATGAAGTCGATCCGTTTCTCAATCTTGTTAAAGGCCGCGGCGGTGCTCTCCTCCGTGAGAAAATGGTGGAAGCCGCTTCTGATAAATTCGTTGTGGTCGTGGACGACACAAAACTCGTCTCCGGTCTCGGTGGAAGCGGTTTGGCGATGCCGGTGGAGGTTGTTCAGTTTTGTTGGAAATATAATTTGATTAGGCTTCAAGAATTGTTCAAAGAAGAAGGTGTTGATGCGAAATTGAGAGTGGATGAAAGTGGAAAACCATATGTTACTGATAATTCTAATTACATTGTTGATTTGTATTTTAAGACTCCCATCAGAGATGCAAATGCTGCTGGAGCTGAGATTTCGTCGCTTGAAGGTGTCGTCGAGCATGGTTTGTTCTTGAACATGGCTACCTCCGTCATCATTGCTGGTAAAACTGGTGTTGAAGTGAAAGACAAGTGA

Protein sequence

>MsG0780041637.01.T01

MVLGLGTGSTAAFVVSKLGELLNSGELTNIIGVPTSKRTEEQARSLGIPLSVLDDNPRLDLAIDGADEVDPFLNLVKGRGGALLREKMVEAASDKFVVVVDDTKLVSGLGGSGLAMPVEVVQFCWKYNLIRLQELFKEEGVDAKLRVDESGKPYVTDNSNYIVDLYFKTPIRDANAAGAEISSLEGVVEHGLFLNMATSVIIAGKTGVEVKDK*