AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039839.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039839.01.T01 MTR_7g080250 97.403 77 2 0 33 109 106 182 3.55e-50 157
MsG0780039839.01.T01 MTR_5g021180 41.892 74 42 1 31 104 40 112 1.23e-14 65.1
MsG0780039839.01.T01 MTR_1g023140 45.205 73 39 1 31 103 38 109 3.91e-14 63.5
MsG0780039839.01.T01 MTR_3g112410 43.836 73 40 1 31 103 38 109 3.08e-13 61.2
MsG0780039839.01.T01 MTR_7g009070 36.486 74 46 1 26 99 53 125 4.37e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039839.01.T01 AT5G16400 85.714 77 11 0 33 109 109 185 4.28e-43 139
MsG0780039839.01.T01 AT3G02730 87.013 77 10 0 33 109 99 175 4.39e-43 139
MsG0780039839.01.T01 AT3G51030 43.243 74 41 1 26 99 33 105 1.52e-14 64.7
MsG0780039839.01.T01 AT5G39950 38.095 84 49 2 26 108 52 133 8.27e-13 60.5
MsG0780039839.01.T01 AT5G42980 37.143 70 43 1 31 100 37 105 9.48e-13 60.1
MsG0780039839.01.T01 AT1G45145 36.232 69 43 1 31 99 37 104 2.39e-12 58.9
MsG0780039839.01.T01 AT1G19730 38.571 70 41 2 31 99 38 106 1.65e-11 57.0

Find 32 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTAATTCTTTGTATTT+TGG 0.153277 7:-69604939 None:intergenic
AGAATTAGCTGAGAAGTATT+TGG 0.241894 7:+69604950 MsG0780039839.01.T01:CDS
CACCTTAATTCCTAGCTCTT+TGG 0.269465 7:-69605110 None:intergenic
TAATCCATACCTTGTTATCT+TGG 0.303298 7:-69605001 None:intergenic
TGGAGCTAAATATGATGATT+TGG 0.335332 7:+69605183 MsG0780039839.01.T01:CDS
GGCCAAAGAGCTAGGAATTA+AGG 0.347175 7:+69605108 MsG0780039839.01.T01:CDS
TGATCATGGTCTATCTATAA+AGG 0.357562 7:+69605427 MsG0780039839.01.T01:three_prime_UTR
TATACAAGCACACCCTGATT+TGG 0.360216 7:-69604424 None:intergenic
GATGTACATGTAGCCAAATC+AGG 0.373944 7:+69604411 MsG0780039839.01.T01:CDS
CTGATGACCACGCCTTGAAT+TGG 0.385789 7:-69604353 None:intergenic
TTCTGTTATCAACTTGATCA+TGG 0.406946 7:+69605413 MsG0780039839.01.T01:three_prime_UTR
AAGGTTGTAAAAGAAGTAAC+TGG 0.408021 7:+69605163 MsG0780039839.01.T01:CDS
AATTCCTAGCTCTTTGGCCA+AGG 0.431968 7:-69605104 None:intergenic
ATTCCTAGCTCTTTGGCCAA+GGG 0.487064 7:-69605103 None:intergenic
GTTTAGCAAAGACCAATTCA+AGG 0.498913 7:+69604341 MsG0780039839.01.T01:CDS
CAGCCCTTGGCCAAAGAGCT+AGG 0.508539 7:+69605100 MsG0780039839.01.T01:intron
GCTAGAACGAACTGTGTCAA+TGG 0.511358 7:-69605212 None:intergenic
ATTGATTATTCCCTATGCAT+AGG 0.511444 7:+69605370 MsG0780039839.01.T01:three_prime_UTR
TTTGGAGCTATCACTTTGCA+AGG 0.518801 7:-69604921 None:intergenic
TTGATTATTCCCTATGCATA+GGG 0.522813 7:+69605371 MsG0780039839.01.T01:three_prime_UTR
TGCATTAGAAATACTTACAG+AGG 0.576470 7:-69605288 None:intergenic
ATGTACATGTAGCCAAATCA+GGG 0.577134 7:+69604412 MsG0780039839.01.T01:CDS
GCAAAGACCAATTCAAGGCG+TGG 0.603598 7:+69604346 MsG0780039839.01.T01:CDS
GCATTAGAAATACTTACAGA+GGG 0.629622 7:-69605287 None:intergenic
TAAAATTCTGAAAGACAACA+AGG 0.632667 7:+69605144 MsG0780039839.01.T01:CDS
CAAGGCGTGGTCATCAGCTG+AGG 0.646940 7:+69604359 MsG0780039839.01.T01:CDS
CAAAGAGCTAGGAATTAAGG+TGG 0.670646 7:+69605111 MsG0780039839.01.T01:CDS
TGATTGCAACCAAGATAACA+AGG 0.674067 7:+69604992 MsG0780039839.01.T01:CDS
TGCAATTGTAAGCTGCTGAG+TGG 0.681997 7:+69605307 MsG0780039839.01.T01:three_prime_UTR
TCATGGTCTATCTATAAAGG+TGG 0.684533 7:+69605430 MsG0780039839.01.T01:three_prime_UTR
TACAGCTTACAACAACAACA+TGG 0.685248 7:-69605347 None:intergenic
AAGCTGCTGAGTGGAAGATG+AGG 0.731291 7:+69605316 MsG0780039839.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTAGTTTTTTAAAA+AGG + Chr7:69604598-69604617 MsG0780039839.01.T01:intron 10.0%
!!! TTTCATTAGTTTTTTAAAAA+GGG + Chr7:69604599-69604618 MsG0780039839.01.T01:intron 10.0%
!! GATAAAAAGATTAATTGATT+TGG + Chr7:69604661-69604680 MsG0780039839.01.T01:intron 15.0%
!! CTAAATATAAAGTGTAGATT+TGG + Chr7:69604856-69604875 MsG0780039839.01.T01:intron 20.0%
!!! ATGATATTTTTACATAGCAA+AGG - Chr7:69605259-69605278 None:intergenic 20.0%
!!! TCTTTCAGAATTTTAAAAGT+GGG - Chr7:69605139-69605158 None:intergenic 20.0%
!!! TGATATTTTTACATAGCAAA+GGG - Chr7:69605258-69605277 None:intergenic 20.0%
! ACATAGTGTTTAATGGTTAT+TGG + Chr7:69605077-69605096 MsG0780039839.01.T01:intron 25.0%
! TAAACTCAAAAATCAATCTC+TGG + Chr7:69604759-69604778 MsG0780039839.01.T01:intron 25.0%
! TTCATTGTAAATCATATCCA+AGG - Chr7:69604886-69604905 None:intergenic 25.0%
!! TAAAATTCTGAAAGACAACA+AGG + Chr7:69605144-69605163 MsG0780039839.01.T01:CDS 25.0%
!! TCAGCTAATTCTTTGTATTT+TGG - Chr7:69604942-69604961 None:intergenic 25.0%
!! TGATTTACAATGAATTTTGC+AGG + Chr7:69604892-69604911 MsG0780039839.01.T01:intron 25.0%
!!! GTCTTTCAGAATTTTAAAAG+TGG - Chr7:69605140-69605159 None:intergenic 25.0%
!!! GTGTTATTGACTTATGTTTT+AGG + Chr7:69604384-69604403 MsG0780039839.01.T01:CDS 25.0%
AAACTAATGAAATGATCAGC+TGG - Chr7:69604591-69604610 None:intergenic 30.0%
AAGGTTGTAAAAGAAGTAAC+TGG + Chr7:69605163-69605182 MsG0780039839.01.T01:CDS 30.0%
ACACTTTATATTTAGCAGCA+GGG - Chr7:69604851-69604870 None:intergenic 30.0%
ACTGAATAGTAGATAGTCTA+TGG + Chr7:69605032-69605051 MsG0780039839.01.T01:intron 30.0%
ATTGATTATTCCCTATGCAT+AGG + Chr7:69605370-69605389 MsG0780039839.01.T01:three_prime_UTR 30.0%
CATGATCCTTATAATAGTCA+TGG - Chr7:69604798-69604817 None:intergenic 30.0%
GCATTAGAAATACTTACAGA+GGG - Chr7:69605290-69605309 None:intergenic 30.0%
TAACCATTAAACACTATGTC+AGG - Chr7:69605076-69605095 None:intergenic 30.0%
TACACTTTATATTTAGCAGC+AGG - Chr7:69604852-69604871 None:intergenic 30.0%
TGATCATGGTCTATCTATAA+AGG + Chr7:69605427-69605446 MsG0780039839.01.T01:three_prime_UTR 30.0%
TGCATTAGAAATACTTACAG+AGG - Chr7:69605291-69605310 None:intergenic 30.0%
TGGAGCTAAATATGATGATT+TGG + Chr7:69605183-69605202 MsG0780039839.01.T01:CDS 30.0%
TTCTGTTATCAACTTGATCA+TGG + Chr7:69605413-69605432 MsG0780039839.01.T01:three_prime_UTR 30.0%
TTGATTATTCCCTATGCATA+GGG + Chr7:69605371-69605390 MsG0780039839.01.T01:three_prime_UTR 30.0%
! TAATCCATACCTTGTTATCT+TGG - Chr7:69605004-69605023 None:intergenic 30.0%
!! AGAATTAGCTGAGAAGTATT+TGG + Chr7:69604950-69604969 MsG0780039839.01.T01:CDS 30.0%
AGTGTAGATTTGGATAACCT+TGG + Chr7:69604866-69604885 MsG0780039839.01.T01:intron 35.0%
ATAATCCACTCTTATGCACA+TGG - Chr7:69604517-69604536 None:intergenic 35.0%
ATGTACATGTAGCCAAATCA+GGG + Chr7:69604412-69604431 MsG0780039839.01.T01:CDS 35.0%
GGATTATTCATGACTCATGA+GGG + Chr7:69604530-69604549 MsG0780039839.01.T01:intron 35.0%
GTTTAGCAAAGACCAATTCA+AGG + Chr7:69604341-69604360 MsG0780039839.01.T01:CDS 35.0%
TACAGCTTACAACAACAACA+TGG - Chr7:69605350-69605369 None:intergenic 35.0%
TCAAAAATCAATCTCTGGTG+TGG + Chr7:69604764-69604783 MsG0780039839.01.T01:intron 35.0%
TCATGGTCTATCTATAAAGG+TGG + Chr7:69605430-69605449 MsG0780039839.01.T01:three_prime_UTR 35.0%
TGATTGCAACCAAGATAACA+AGG + Chr7:69604992-69605011 MsG0780039839.01.T01:CDS 35.0%
TGGATTATTCATGACTCATG+AGG + Chr7:69604529-69604548 MsG0780039839.01.T01:intron 35.0%
TTGCAGCCATGACTATTATA+AGG + Chr7:69604789-69604808 MsG0780039839.01.T01:intron 35.0%
! TACAATGAATTTTGCAGGTG+TGG + Chr7:69604897-69604916 MsG0780039839.01.T01:intron 35.0%
!! CAGGGATAAAAGTGTCATAA+GGG - Chr7:69604833-69604852 None:intergenic 35.0%
CAAAGAGCTAGGAATTAAGG+TGG + Chr7:69605111-69605130 MsG0780039839.01.T01:CDS 40.0%
GATGTACATGTAGCCAAATC+AGG + Chr7:69604411-69604430 MsG0780039839.01.T01:CDS 40.0%
GCAACCAAGATAACAAGGTA+TGG + Chr7:69604997-69605016 MsG0780039839.01.T01:intron 40.0%
TATACAAGCACACCCTGATT+TGG - Chr7:69604427-69604446 None:intergenic 40.0%
TCAAACTCTGATGTACCACT+GGG + Chr7:69604694-69604713 MsG0780039839.01.T01:intron 40.0%
TGGCCTGACATAGTGTTTAA+TGG + Chr7:69605070-69605089 MsG0780039839.01.T01:intron 40.0%
TTTCTCCATGTGCATAAGAG+TGG + Chr7:69604509-69604528 MsG0780039839.01.T01:intron 40.0%
! AGCAACTTTTCCCTATGCAT+AGG - Chr7:69605384-69605403 None:intergenic 40.0%
! CACCTTAATTCCTAGCTCTT+TGG - Chr7:69605113-69605132 None:intergenic 40.0%
! GCAACTTTTCCCTATGCATA+GGG - Chr7:69605383-69605402 None:intergenic 40.0%
! TTTGGAGCTATCACTTTGCA+AGG - Chr7:69604924-69604943 None:intergenic 40.0%
!! GCAGGGATAAAAGTGTCATA+AGG - Chr7:69604834-69604853 None:intergenic 40.0%
CAAACTCTGATGTACCACTG+GGG + Chr7:69604695-69604714 MsG0780039839.01.T01:intron 45.0%
CAGCTGGCTGATCACTATTT+AGG - Chr7:69604575-69604594 None:intergenic 45.0%
CATGACTCATGAGGGTTGTT+GGG + Chr7:69604538-69604557 MsG0780039839.01.T01:intron 45.0%
CTCAAACTCTGATGTACCAC+TGG + Chr7:69604693-69604712 MsG0780039839.01.T01:intron 45.0%
GCTAGAACGAACTGTGTCAA+TGG - Chr7:69605215-69605234 None:intergenic 45.0%
GGCCAAAGAGCTAGGAATTA+AGG + Chr7:69605108-69605127 MsG0780039839.01.T01:CDS 45.0%
TAATGGTTATTGGCAGCCCT+TGG + Chr7:69605087-69605106 MsG0780039839.01.T01:intron 45.0%
TATGGTTGCCCATGCTTTCT+TGG + Chr7:69605050-69605069 MsG0780039839.01.T01:intron 45.0%
TATGTCAGGCCAAGAAAGCA+TGG - Chr7:69605062-69605081 None:intergenic 45.0%
TCATGACTCATGAGGGTTGT+TGG + Chr7:69604537-69604556 MsG0780039839.01.T01:intron 45.0%
TGCAATTGTAAGCTGCTGAG+TGG + Chr7:69605307-69605326 MsG0780039839.01.T01:three_prime_UTR 45.0%
! AATTCCTAGCTCTTTGGCCA+AGG - Chr7:69605107-69605126 None:intergenic 45.0%
! ATGTCAGGCCAAGAAAGCAT+GGG - Chr7:69605061-69605080 None:intergenic 45.0%
! ATTCCTAGCTCTTTGGCCAA+GGG - Chr7:69605106-69605125 None:intergenic 45.0%
AAGCTGCTGAGTGGAAGATG+AGG + Chr7:69605316-69605335 MsG0780039839.01.T01:three_prime_UTR 50.0%
AGTTGCTGCGCAATGTGTGT+GGG + Chr7:69604730-69604749 MsG0780039839.01.T01:intron 50.0%
CTGATGACCACGCCTTGAAT+TGG - Chr7:69604356-69604375 None:intergenic 50.0%
GCAAAGACCAATTCAAGGCG+TGG + Chr7:69604346-69604365 MsG0780039839.01.T01:CDS 50.0%
TAGTTGCTGCGCAATGTGTG+TGG + Chr7:69604729-69604748 MsG0780039839.01.T01:intron 50.0%
CAAGGCGTGGTCATCAGCTG+AGG + Chr7:69604359-69604378 MsG0780039839.01.T01:CDS 60.0%
CAGCCCTTGGCCAAAGAGCT+AGG + Chr7:69605100-69605119 MsG0780039839.01.T01:intron 60.0%
CTACTAGCGCTAGACCCCAG+TGG - Chr7:69604712-69604731 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 69604339 69605510 69604339 ID=MsG0780039839.01;Name=MsG0780039839.01
Chr7 mRNA 69604339 69605510 69604339 ID=MsG0780039839.01.T01;Parent=MsG0780039839.01;Name=MsG0780039839.01.T01;_AED=0.47;_eAED=0.56;_QI=0|0.5|0.66|1|1|1|3|273|109
Chr7 exon 69604339 69604433 69604339 ID=MsG0780039839.01.T01:exon:12586;Parent=MsG0780039839.01.T01
Chr7 exon 69604914 69605013 69604914 ID=MsG0780039839.01.T01:exon:12587;Parent=MsG0780039839.01.T01
Chr7 exon 69605103 69605510 69605103 ID=MsG0780039839.01.T01:exon:12588;Parent=MsG0780039839.01.T01
Chr7 CDS 69604339 69604433 69604339 ID=MsG0780039839.01.T01:cds;Parent=MsG0780039839.01.T01
Chr7 CDS 69604914 69605013 69604914 ID=MsG0780039839.01.T01:cds;Parent=MsG0780039839.01.T01
Chr7 CDS 69605103 69605237 69605103 ID=MsG0780039839.01.T01:cds;Parent=MsG0780039839.01.T01
Chr7 three_prime_UTR 69605238 69605510 69605238 ID=MsG0780039839.01.T01:three_prime_utr;Parent=MsG0780039839.01.T01
Gene Sequence

>MsG0780039839.01.T01

ATGTTTAGCAAAGACCAATTCAAGGCGTGGTCATCAGCTGAGGTTGTGTTATTGACTTATGTTTTAGGATATGATGTACATGTAGCCAAATCAGGGTGTGGTCCTTGCAAAGTGATAGCTCCAAAATACAAAGAATTAGCTGAGAAGTATTTGGATGTTGTCTTTCTAAAGCTTGATTGCAACCAAGATAACAAGCCCTTGGCCAAAGAGCTAGGAATTAAGGTGGTTCCCACTTTTAAAATTCTGAAAGACAACAAGGTTGTAAAAGAAGTAACTGGAGCTAAATATGATGATTTGGTCTTTGCCATTGACACAGTTCGTTCTAGCTAA

Protein sequence

>MsG0780039839.01.T01

MFSKDQFKAWSSAEVVLLTYVLGYDVHVAKSGCGPCKVIAPKYKELAEKYLDVVFLKLDCNQDNKPLAKELGIKVVPTFKILKDNKVVKEVTGAKYDDLVFAIDTVRSS*