Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016498.01.T01 | XP_003602205.1 | 100 | 74 | 0 | 0 | 1 | 74 | 126 | 199 | 3.90E-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016498.01.T01 | Q6EN42 | 81.818 | 66 | 12 | 0 | 1 | 66 | 139 | 204 | 7.40E-34 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016498.01.T01 | G7J6S4 | 100.000 | 74 | 0 | 0 | 1 | 74 | 126 | 199 | 1.86e-45 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001663.01 | MsG0380016498.01 | 0.809618 | 1.731197e-50 | 1.021395e-47 |
| MsG0180002955.01 | MsG0380016498.01 | 0.800081 | 1.703552e-48 | 7.860664e-46 |
| MsG0180003510.01 | MsG0380016498.01 | 0.803445 | 3.473741e-49 | 1.745952e-46 |
| MsG0180003864.01 | MsG0380016498.01 | 0.819225 | 1.298644e-52 | 9.934165e-50 |
| MsG0180004996.01 | MsG0380016498.01 | 0.818905 | 1.535546e-52 | 1.164430e-49 |
| MsG0180005160.01 | MsG0380016498.01 | 0.813505 | 2.474648e-51 | 1.619025e-48 |
| MsG0180006016.01 | MsG0380016498.01 | 0.816051 | 6.748194e-52 | 4.731668e-49 |
| MsG0180006138.01 | MsG0380016498.01 | 0.802375 | 5.779934e-49 | 2.826925e-46 |
| MsG0280006356.01 | MsG0380016498.01 | 0.810439 | 1.152002e-50 | 6.943983e-48 |
| MsG0280006440.01 | MsG0380016498.01 | 0.812288 | 4.571629e-51 | 2.895103e-48 |
| MsG0280010722.01 | MsG0380016498.01 | 0.806156 | 9.433141e-50 | 5.083421e-47 |
| MsG0280011235.01 | MsG0380016498.01 | 0.810643 | 1.040820e-50 | 6.308341e-48 |
| MsG0380011537.01 | MsG0380016498.01 | 0.812675 | 3.763298e-51 | 2.407730e-48 |
| MsG0380011895.01 | MsG0380016498.01 | 0.823396 | 1.416439e-53 | 1.218013e-50 |
| MsG0380015157.01 | MsG0380016498.01 | 0.801595 | 8.359760e-49 | 4.008002e-46 |
| MsG0380016498.01 | MsG0480021240.01 | 0.829093 | 6.242193e-55 | 6.324248e-52 |
| MsG0380016498.01 | MsG0480021959.01 | 0.801754 | 7.756221e-49 | 3.733658e-46 |
| MsG0380016498.01 | MsG0480022933.01 | 0.803730 | 3.032204e-49 | 1.535239e-46 |
| MsG0380016498.01 | MsG0480023224.01 | 0.800321 | 1.522728e-48 | 7.069310e-46 |
| MsG0380016498.01 | MsG0480023270.01 | 0.800672 | 1.291252e-48 | 6.048340e-46 |
| MsG0380016498.01 | MsG0580024160.01 | 0.815592 | 8.544807e-52 | 5.916130e-49 |
| MsG0380016498.01 | MsG0680035340.01 | 0.810498 | 1.118590e-50 | 6.753415e-48 |
| MsG0380016498.01 | MsG0780038911.01 | 0.834709 | 2.562392e-56 | 3.065132e-53 |
| MsG0380016498.01 | MsG0780039143.01 | 0.822932 | 1.818131e-53 | 1.543343e-50 |
| MsG0380016498.01 | MsG0880042972.01 | 0.822857 | 1.892219e-53 | 1.602799e-50 |
| MsG0380016498.01 | MsG0880044409.01 | 0.813843 | 2.084193e-51 | 1.376072e-48 |
| MsG0380016498.01 | MsG0880046058.01 | 0.801016 | 1.098725e-48 | 5.191840e-46 |
| MsG0380016498.01 | MsG0880046077.01 | 0.806995 | 6.275270e-50 | 3.455072e-47 |
| MsG0380016498.01 | MsG0880046244.01 | 0.800089 | 1.697337e-48 | 7.833498e-46 |
| MsG0380016498.01 | MsG0880046733.01 | 0.829837 | 4.115948e-55 | 4.262688e-52 |
| MsG0280007023.01 | MsG0380016498.01 | 0.823445 | 1.380225e-53 | 1.188600e-50 |
| MsG0280010416.01 | MsG0380016498.01 | 0.807899 | 4.034701e-50 | 2.274284e-47 |
| MsG0280010531.01 | MsG0380016498.01 | 0.817263 | 3.610908e-52 | 2.616932e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016498.01.T01 | MTR_3g090980 | 100.000 | 74 | 0 | 0 | 1 | 74 | 126 | 199 | 4.72e-49 | 153 |
| MsG0380016498.01.T01 | MTR_1g028380 | 86.567 | 67 | 9 | 0 | 1 | 67 | 124 | 190 | 3.31e-38 | 125 |
| MsG0380016498.01.T01 | MTR_8g027805 | 76.923 | 65 | 15 | 0 | 1 | 65 | 123 | 187 | 1.14e-32 | 111 |
| MsG0380016498.01.T01 | MTR_5g083270 | 60.000 | 45 | 18 | 0 | 9 | 53 | 159 | 203 | 1.78e-15 | 67.4 |
| MsG0380016498.01.T01 | MTR_3g071740 | 65.000 | 40 | 14 | 0 | 14 | 53 | 183 | 222 | 1.45e-14 | 65.5 |
| MsG0380016498.01.T01 | MTR_4g014460 | 43.396 | 53 | 30 | 0 | 2 | 54 | 156 | 208 | 6.38e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016498.01.T01 | AT5G53160 | 85.484 | 62 | 9 | 0 | 1 | 62 | 124 | 185 | 5.37e-32 | 109 |
| MsG0380016498.01.T01 | AT1G01360 | 77.419 | 62 | 14 | 0 | 1 | 62 | 126 | 187 | 1.58e-30 | 105 |
| MsG0380016498.01.T01 | AT4G01026 | 70.312 | 64 | 19 | 0 | 1 | 64 | 128 | 191 | 4.18e-28 | 100 |
| MsG0380016498.01.T01 | AT4G27920 | 77.358 | 53 | 12 | 0 | 1 | 53 | 120 | 172 | 1.67e-24 | 90.5 |
| MsG0380016498.01.T01 | AT2G40330 | 52.174 | 46 | 22 | 0 | 9 | 54 | 165 | 210 | 8.90e-13 | 60.8 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCTTCCATCAATAATTTC+TGG | 0.156182 | 3:+84329076 | None:intergenic |
| TTTGAGATTGCACTTAATTA+AGG | 0.261510 | 3:+84328973 | None:intergenic |
| GTCTTCCATCAATAATTTCT+GGG | 0.334881 | 3:+84329077 | None:intergenic |
| GATTCAATTACCAGGGTTCC+TGG | 0.344614 | 3:+84329055 | None:intergenic |
| CTCGGAAGGGCATGCTGTGC+AGG | 0.389173 | 3:-84328936 | MsG0380016498.01.T01:CDS |
| CTCCACCCAGAAATTATTGA+TGG | 0.415182 | 3:-84329082 | MsG0380016498.01.T01:CDS |
| TCATCCTTGGTATTCCCCTC+TGG | 0.450075 | 3:+84329016 | None:intergenic |
| CCACAAAAGATTCAATTACC+AGG | 0.481100 | 3:+84329047 | None:intergenic |
| CATCAATAATTTCTGGGTGG+AGG | 0.490844 | 3:+84329083 | None:intergenic |
| ATCAATAATTTCTGGGTGGA+GGG | 0.509205 | 3:+84329084 | None:intergenic |
| CAAATCACTTTCAGATGTCT+CGG | 0.517164 | 3:-84328954 | MsG0380016498.01.T01:CDS |
| ACCTCACTGAACCACTTGAT+CGG | 0.518102 | 3:-84328913 | MsG0380016498.01.T01:CDS |
| TAAGGCTTCAACGAAGTAGC+AGG | 0.523056 | 3:+84328991 | None:intergenic |
| GAAGTAGCAGGTCTCATCCT+TGG | 0.540678 | 3:+84329003 | None:intergenic |
| GAAATTATTGATGGAAGACC+AGG | 0.551294 | 3:-84329073 | MsG0380016498.01.T01:CDS |
| AACTCATGCATCCGATCAAG+TGG | 0.554489 | 3:+84328902 | None:intergenic |
| TCACTTTCAGATGTCTCGGA+AGG | 0.579393 | 3:-84328950 | MsG0380016498.01.T01:CDS |
| ATGCATGAGTTGTTGATAAG+TGG | 0.595471 | 3:-84328890 | MsG0380016498.01.T01:CDS |
| CACAAAAGATTCAATTACCA+GGG | 0.601688 | 3:+84329048 | None:intergenic |
| CACTTTCAGATGTCTCGGAA+GGG | 0.603305 | 3:-84328949 | MsG0380016498.01.T01:CDS |
| TGATGGAAGACCAGGAACCC+TGG | 0.609392 | 3:-84329065 | MsG0380016498.01.T01:CDS |
| TTTGTGGTAGATGTACCAGA+GGG | 0.648012 | 3:-84329031 | MsG0380016498.01.T01:CDS |
| TTCCATCAATAATTTCTGGG+TGG | 0.663028 | 3:+84329080 | None:intergenic |
| TCCGATCAAGTGGTTCAGTG+AGG | 0.707128 | 3:+84328912 | None:intergenic |
| TGTACCAGAGGGGAATACCA+AGG | 0.724913 | 3:-84329020 | MsG0380016498.01.T01:CDS |
| TTGTGGTAGATGTACCAGAG+GGG | 0.770632 | 3:-84329030 | MsG0380016498.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGAGATTGCACTTAATTA+AGG | + | Chr3:84329004-84329023 | None:intergenic | 25.0% |
| CACAAAAGATTCAATTACCA+GGG | + | Chr3:84328929-84328948 | None:intergenic | 30.0% | |
| GTCTTCCATCAATAATTTCT+GGG | + | Chr3:84328900-84328919 | None:intergenic | 30.0% | |
| CAAATCACTTTCAGATGTCT+CGG | - | Chr3:84329020-84329039 | MsG0380016498.01.T01:CDS | 35.0% | |
| CCACAAAAGATTCAATTACC+AGG | + | Chr3:84328930-84328949 | None:intergenic | 35.0% | |
| GAAATTATTGATGGAAGACC+AGG | - | Chr3:84328901-84328920 | MsG0380016498.01.T01:CDS | 35.0% | |
| GGTCTTCCATCAATAATTTC+TGG | + | Chr3:84328901-84328920 | None:intergenic | 35.0% | |
| ! | ATCAATAATTTCTGGGTGGA+GGG | + | Chr3:84328893-84328912 | None:intergenic | 35.0% |
| ! | ATGCATGAGTTGTTGATAAG+TGG | - | Chr3:84329084-84329103 | MsG0380016498.01.T01:CDS | 35.0% |
| ! | CCTGGTAATTGAATCTTTTG+TGG | - | Chr3:84328927-84328946 | MsG0380016498.01.T01:CDS | 35.0% |
| ! | TTCCATCAATAATTTCTGGG+TGG | + | Chr3:84328897-84328916 | None:intergenic | 35.0% |
| CTCCACCCAGAAATTATTGA+TGG | - | Chr3:84328892-84328911 | MsG0380016498.01.T01:CDS | 40.0% | |
| TTTGTGGTAGATGTACCAGA+GGG | - | Chr3:84328943-84328962 | MsG0380016498.01.T01:CDS | 40.0% | |
| ! | CATCAATAATTTCTGGGTGG+AGG | + | Chr3:84328894-84328913 | None:intergenic | 40.0% |
| ! | TTTTGTGGTAGATGTACCAG+AGG | - | Chr3:84328942-84328961 | MsG0380016498.01.T01:CDS | 40.0% |
| AACTCATGCATCCGATCAAG+TGG | + | Chr3:84329075-84329094 | None:intergenic | 45.0% | |
| ACCTCACTGAACCACTTGAT+CGG | - | Chr3:84329061-84329080 | MsG0380016498.01.T01:CDS | 45.0% | |
| CACTTTCAGATGTCTCGGAA+GGG | - | Chr3:84329025-84329044 | MsG0380016498.01.T01:CDS | 45.0% | |
| GATTCAATTACCAGGGTTCC+TGG | + | Chr3:84328922-84328941 | None:intergenic | 45.0% | |
| TAAGGCTTCAACGAAGTAGC+AGG | + | Chr3:84328986-84329005 | None:intergenic | 45.0% | |
| TCACTTTCAGATGTCTCGGA+AGG | - | Chr3:84329024-84329043 | MsG0380016498.01.T01:CDS | 45.0% | |
| TTGTGGTAGATGTACCAGAG+GGG | - | Chr3:84328944-84328963 | MsG0380016498.01.T01:CDS | 45.0% | |
| GAAGTAGCAGGTCTCATCCT+TGG | + | Chr3:84328974-84328993 | None:intergenic | 50.0% | |
| TCATCCTTGGTATTCCCCTC+TGG | + | Chr3:84328961-84328980 | None:intergenic | 50.0% | |
| TCCGATCAAGTGGTTCAGTG+AGG | + | Chr3:84329065-84329084 | None:intergenic | 50.0% | |
| TGTACCAGAGGGGAATACCA+AGG | - | Chr3:84328954-84328973 | MsG0380016498.01.T01:CDS | 50.0% | |
| TGATGGAAGACCAGGAACCC+TGG | - | Chr3:84328909-84328928 | MsG0380016498.01.T01:CDS | 55.0% | |
| ! | CTCGGAAGGGCATGCTGTGC+AGG | - | Chr3:84329038-84329057 | MsG0380016498.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 84328886 | 84329110 | 84328886 | ID=MsG0380016498.01;Name=MsG0380016498.01 |
| Chr3 | mRNA | 84328886 | 84329110 | 84328886 | ID=MsG0380016498.01.T01;Parent=MsG0380016498.01;Name=MsG0380016498.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|74 |
| Chr3 | exon | 84328886 | 84329110 | 84328886 | ID=MsG0380016498.01.T01:exon:14261;Parent=MsG0380016498.01.T01 |
| Chr3 | CDS | 84328886 | 84329110 | 84328886 | ID=MsG0380016498.01.T01:cds;Parent=MsG0380016498.01.T01 |
| Gene Sequence |
| Protein sequence |
