AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015157.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015157.01.T01 MTR_3g063970 89.610 77 2 1 1 77 1 71 7.49e-43 133
MsG0380015157.01.T01 MTR_3g063970 95.833 48 2 0 1 48 1 48 3.21e-15 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015157.01.T01 AT3G14430 60.870 69 20 2 9 77 15 76 2.14e-22 82.0

Find 27 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACATCAATTCCTTTAAA+AGG 0.150864 3:-65964510 None:intergenic
ATTTCTTACGAAGGCGAAAA+TGG 0.322719 3:-65963490 None:intergenic
GTTAATGTTACATTCTAGTT+AGG 0.334854 3:+65964706 MsG0380015157.01.T01:three_prime_UTR
ACAAATACTTGATTGTATTC+AGG 0.336136 3:-65964455 None:intergenic
GTATCTTTATGTAAGTTTGT+AGG 0.358607 3:+65964649 MsG0380015157.01.T01:three_prime_UTR
CTGGTGGTGGACCAGAGCTT+TGG 0.389186 3:+65964486 MsG0380015157.01.T01:CDS
CTTTAAAAGGGCCAAAGCTC+TGG 0.402112 3:-65964497 None:intergenic
AAACATCAATTCCTTTAAAA+GGG 0.443719 3:-65964509 None:intergenic
AAAGGAATTGATGTTTGGAC+AGG 0.475928 3:+65964516 MsG0380015157.01.T01:exon
CAAAGCTCTGGTCCACCACC+AGG 0.488950 3:-65964485 None:intergenic
CAAGTATTTGTGAAGAAGCC+TGG 0.508253 3:+65964467 MsG0380015157.01.T01:CDS
GACAGGTTGGACGTCATCCA+TGG 0.522357 3:+65964533 MsG0380015157.01.T01:three_prime_UTR
CATAAAGATACAAATACAAA+CGG 0.528427 3:-65964637 None:intergenic
GAATTGATGTTTGGACAGGT+TGG 0.543485 3:+65964520 MsG0380015157.01.T01:three_prime_UTR
CTAGACGGCGAGTCGAGACT+CGG 0.545765 3:+65963436 MsG0380015157.01.T01:CDS
TGAAGAAGAAAAGAAAGAAG+AGG 0.565354 3:-65963527 None:intergenic
GAAATATGTAATATCATCAC+AGG 0.573101 3:-65964615 None:intergenic
AGAGAACAGCGATGGCTAGA+CGG 0.584999 3:+65963421 None:intergenic
AAAATATGTGAAATCATCAC+AGG 0.600529 3:-65964574 None:intergenic
TTTGTGAAGAAGCCTGGTGG+TGG 0.604245 3:+65964473 MsG0380015157.01.T01:CDS
ATTAACATCATTCAAATCTG+TGG 0.614330 3:-65964689 None:intergenic
GGGACGAGTCGTTATTGACT+CGG 0.618106 3:+65963457 MsG0380015157.01.T01:CDS
TAGACGGCGAGTCGAGACTC+GGG 0.629156 3:+65963437 MsG0380015157.01.T01:CDS
AAACTCAGCATTTCTTACGA+AGG 0.638252 3:-65963499 None:intergenic
GCATGCTTAGAATACCTCCA+TGG 0.699909 3:-65964550 None:intergenic
AGGTTGGACGTCATCCATGG+AGG 0.746774 3:+65964536 MsG0380015157.01.T01:three_prime_UTR
GTATTTGTGAAGAAGCCTGG+TGG 0.787083 3:+65964470 MsG0380015157.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATAAAAAGAAAAAAC+AGG - Chr3:65963594-65963613 None:intergenic 10.0%
!! AATAATAAAAAGAAAAAACA+GGG - Chr3:65963593-65963612 None:intergenic 10.0%
!!! TTTTCATGTTTTGTTAAATT+AGG + Chr3:65963679-65963698 MsG0380015157.01.T01:intron 15.0%
!! ACATAATTCAAAGTAATCAT+AGG + Chr3:65964189-65964208 MsG0380015157.01.T01:intron 20.0%
!! ATAATTCATTCATATATGGT+TGG + Chr3:65964288-65964307 MsG0380015157.01.T01:intron 20.0%
!! CATAAAGATACAAATACAAA+CGG - Chr3:65964640-65964659 None:intergenic 20.0%
!! CTTCATAATTCATTCATATA+TGG + Chr3:65964284-65964303 MsG0380015157.01.T01:intron 20.0%
!!! AAACATCAATTCCTTTAAAA+GGG - Chr3:65964512-65964531 None:intergenic 20.0%
!!! ATATTCACAAGTTTTTATGT+CGG + Chr3:65964002-65964021 MsG0380015157.01.T01:intron 20.0%
!!! TTTTGATAACTGGAAATTTT+TGG + Chr3:65964311-65964330 MsG0380015157.01.T01:intron 20.0%
! AAAATATGTGAAATCATCAC+AGG - Chr3:65964577-65964596 None:intergenic 25.0%
! AAGAACGAAAATTTGAACAA+AGG - Chr3:65963635-65963654 None:intergenic 25.0%
! AATAAAAAGAAAAAACAGGG+AGG - Chr3:65963590-65963609 None:intergenic 25.0%
! AATATGTTTGAAAGAACTGA+TGG - Chr3:65963987-65964006 None:intergenic 25.0%
! ACAAATACTTGATTGTATTC+AGG - Chr3:65964458-65964477 None:intergenic 25.0%
! ATTAACATCATTCAAATCTG+TGG - Chr3:65964692-65964711 None:intergenic 25.0%
! ATTTGAACAAAGGATGTAAA+TGG - Chr3:65963625-65963644 None:intergenic 25.0%
! CAAACATCAATTCCTTTAAA+AGG - Chr3:65964513-65964532 None:intergenic 25.0%
! GAAATATGTAATATCATCAC+AGG - Chr3:65964618-65964637 None:intergenic 25.0%
! GTATCTTTATGTAAGTTTGT+AGG + Chr3:65964649-65964668 MsG0380015157.01.T01:three_prime_UTR 25.0%
! TATGAATGAATTATGAAGCA+CGG - Chr3:65964282-65964301 None:intergenic 25.0%
! TTTGAACAAAGGATGTAAAT+GGG - Chr3:65963624-65963643 None:intergenic 25.0%
!! GTTAATGTTACATTCTAGTT+AGG + Chr3:65964706-65964725 MsG0380015157.01.T01:three_prime_UTR 25.0%
!! TTGTGTTGTAATTTTGCTTT+TGG + Chr3:65964387-65964406 MsG0380015157.01.T01:intron 25.0%
!!! AATTTTCGTTCTTTTTCATG+TGG + Chr3:65963642-65963661 MsG0380015157.01.T01:intron 25.0%
!!! ATTTTCGTTCTTTTTCATGT+GGG + Chr3:65963643-65963662 MsG0380015157.01.T01:intron 25.0%
!!! CTTTTAAAGGAATTGATGTT+TGG + Chr3:65964511-65964530 MsG0380015157.01.T01:exon 25.0%
AACTATATACCAATTCCATC+TGG - Chr3:65963806-65963825 None:intergenic 30.0%
CAAAATTACAACACAAACAG+AGG - Chr3:65964383-65964402 None:intergenic 30.0%
GGTTGGATAGTTATATCAAA+AGG + Chr3:65963766-65963785 MsG0380015157.01.T01:intron 30.0%
TAAATTGAAAACTGCATCAC+TGG + Chr3:65963733-65963752 MsG0380015157.01.T01:intron 30.0%
TGAAGAAGAAAAGAAAGAAG+AGG - Chr3:65963530-65963549 None:intergenic 30.0%
!!! ATATGGTTGGTTTTGATAAC+TGG + Chr3:65964301-65964320 MsG0380015157.01.T01:intron 30.0%
AAACTCAGCATTTCTTACGA+AGG - Chr3:65963502-65963521 None:intergenic 35.0%
AAAGGAATTGATGTTTGGAC+AGG + Chr3:65964516-65964535 MsG0380015157.01.T01:exon 35.0%
AATTACAACACAAACAGAGG+AGG - Chr3:65964380-65964399 None:intergenic 35.0%
ACAATCAGCATGTTTCCTTT+CGG + Chr3:65964076-65964095 MsG0380015157.01.T01:intron 35.0%
ATTACAACACAAACAGAGGA+GGG - Chr3:65964379-65964398 None:intergenic 35.0%
ATTCTGATTCCAGATGGAAT+TGG + Chr3:65963794-65963813 MsG0380015157.01.T01:intron 35.0%
ATTTCTTACGAAGGCGAAAA+TGG - Chr3:65963493-65963512 None:intergenic 35.0%
TTCATTCATGATACACCGAA+AGG - Chr3:65964094-65964113 None:intergenic 35.0%
ACACCAATGTATGTTGACAC+TGG + Chr3:65964233-65964252 MsG0380015157.01.T01:intron 40.0%
CAAGTATTTGTGAAGAAGCC+TGG + Chr3:65964467-65964486 MsG0380015157.01.T01:CDS 40.0%
CACCAATGTATGTTGACACT+GGG + Chr3:65964234-65964253 MsG0380015157.01.T01:intron 40.0%
GAATTGATGTTTGGACAGGT+TGG + Chr3:65964520-65964539 MsG0380015157.01.T01:three_prime_UTR 40.0%
GATAAGCGCTTTAATTGAGC+TGG - Chr3:65963836-65963855 None:intergenic 40.0%
GTAGAGATTCTGATTCCAGA+TGG + Chr3:65963788-65963807 MsG0380015157.01.T01:intron 40.0%
TCATGAATGAAGCACATACG+CGG + Chr3:65964103-65964122 MsG0380015157.01.T01:intron 40.0%
!! TCACTGGTTTGATGATTGGT+TGG + Chr3:65963749-65963768 MsG0380015157.01.T01:intron 40.0%
!! TGCATCACTGGTTTGATGAT+TGG + Chr3:65963745-65963764 MsG0380015157.01.T01:intron 40.0%
GCACGGAAACTTCTCAGATT+CGG - Chr3:65964265-65964284 None:intergenic 45.0%
GCATGCTTAGAATACCTCCA+TGG - Chr3:65964553-65964572 None:intergenic 45.0%
GTATTTGTGAAGAAGCCTGG+TGG + Chr3:65964470-65964489 MsG0380015157.01.T01:CDS 45.0%
GTCCCAGTGTCAACATACAT+TGG - Chr3:65964239-65964258 None:intergenic 45.0%
! CTTTAAAAGGGCCAAAGCTC+TGG - Chr3:65964500-65964519 None:intergenic 45.0%
!! CAGAGCTTTGGCCCTTTTAA+AGG + Chr3:65964498-65964517 MsG0380015157.01.T01:CDS 45.0%
!!! TTATTTTATCAAATTATTAT+TGG - Chr3:65964170-65964189 None:intergenic 5.0%
ACAAACAGAGGAGGGAGTAG+GGG - Chr3:65964371-65964390 None:intergenic 50.0%
ACACAAACAGAGGAGGGAGT+AGG - Chr3:65964373-65964392 None:intergenic 50.0%
ATGAAGCACATACGCGGATG+CGG + Chr3:65964109-65964128 MsG0380015157.01.T01:intron 50.0%
CACAAACAGAGGAGGGAGTA+GGG - Chr3:65964372-65964391 None:intergenic 50.0%
CATAGGTGTCAGTGTCGTGT+TGG + Chr3:65964206-65964225 MsG0380015157.01.T01:intron 50.0%
TTTGTGAAGAAGCCTGGTGG+TGG + Chr3:65964473-65964492 MsG0380015157.01.T01:CDS 50.0%
! GGGACGAGTCGTTATTGACT+CGG + Chr3:65963457-65963476 MsG0380015157.01.T01:CDS 50.0%
AGGTGTCAGTGTCGTGTTGG+TGG + Chr3:65964209-65964228 MsG0380015157.01.T01:intron 55.0%
AGGTTGGACGTCATCCATGG+AGG + Chr3:65964536-65964555 MsG0380015157.01.T01:three_prime_UTR 55.0%
CATCAGACACGACACGGACA+TGG + Chr3:65964133-65964152 MsG0380015157.01.T01:intron 55.0%
TGCGGACATCAGACACGACA+CGG + Chr3:65964127-65964146 MsG0380015157.01.T01:intron 55.0%
! GACAGGTTGGACGTCATCCA+TGG + Chr3:65964533-65964552 MsG0380015157.01.T01:three_prime_UTR 55.0%
! GGTGTCAGTGTCGTGTTGGT+GGG + Chr3:65964210-65964229 MsG0380015157.01.T01:intron 55.0%
ACAGAGGAGGGAGTAGGGGA+TGG - Chr3:65964367-65964386 None:intergenic 60.0%
CGACACGGACATGGACACTC+TGG + Chr3:65964142-65964161 MsG0380015157.01.T01:intron 60.0%
CTAGACGGCGAGTCGAGACT+CGG + Chr3:65963436-65963455 MsG0380015157.01.T01:CDS 60.0%
TAGACGGCGAGTCGAGACTC+GGG + Chr3:65963437-65963456 MsG0380015157.01.T01:CDS 60.0%
! CAAAGCTCTGGTCCACCACC+AGG - Chr3:65964488-65964507 None:intergenic 60.0%
!! CTGGTGGTGGACCAGAGCTT+TGG + Chr3:65964486-65964505 MsG0380015157.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 65963432 65964786 65963432 ID=MsG0380015157.01;Name=MsG0380015157.01
Chr3 mRNA 65963432 65964786 65963432 ID=MsG0380015157.01.T01;Parent=MsG0380015157.01;Name=MsG0380015157.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0.5|1|0|0|2|269|77
Chr3 exon 65963432 65963593 65963432 ID=MsG0380015157.01.T01:exon:22855;Parent=MsG0380015157.01.T01
Chr3 exon 65964446 65964786 65964446 ID=MsG0380015157.01.T01:exon:22856;Parent=MsG0380015157.01.T01
Chr3 CDS 65963432 65963593 65963432 ID=MsG0380015157.01.T01:cds;Parent=MsG0380015157.01.T01
Chr3 CDS 65964446 65964517 65964446 ID=MsG0380015157.01.T01:cds;Parent=MsG0380015157.01.T01
Chr3 three_prime_UTR 65964518 65964786 65964518 ID=MsG0380015157.01.T01:three_prime_utr;Parent=MsG0380015157.01.T01
Gene Sequence

>MsG0380015157.01.T01

ATGGCTAGACGGCGAGTCGAGACTCGGGACGAGTCGTTATTGACTCGGATTGTTGATTCCATTTTCGCCTTCGTAAGAAATGCTGAGTTTGAGATCCTCTTCTTTCTTTTCTTCTTCATCGCTTATATTCTCTTCAAAGACATCGTAAGCTTCTTCCTCCCTACATCAAGACCTGAATACAATCAAGTATTTGTGAAGAAGCCTGGTGGTGGACCAGAGCTTTGGCCCTTTTAA

Protein sequence

>MsG0380015157.01.T01

MARRRVETRDESLLTRIVDSIFAFVRNAEFEILFFLFFFIAYILFKDIVSFFLPTSRPEYNQVFVKKPGGGPELWPF*