AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019151.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019151.01.T01 MTR_4g024000 81.481 216 23 2 1 216 100 298 5.11e-120 343
MsG0480019151.01.T01 MTR_4g102250 58.088 136 54 2 2 137 165 297 7.44e-45 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019151.01.T01 AT2G45380 50.000 214 78 4 3 216 91 275 3.46e-54 175
MsG0480019151.01.T01 AT2G45380 49.767 215 79 4 2 216 130 315 6.92e-54 175
MsG0480019151.01.T01 AT2G45380 49.767 215 79 4 2 216 309 494 1.74e-52 176
MsG0480019151.01.T01 AT2G45380 49.510 204 77 3 13 216 9 186 1.29e-51 165
MsG0480019151.01.T01 AT4G22740 66.667 93 31 0 45 137 206 298 1.57e-39 139
MsG0480019151.01.T01 AT4G22740 66.667 93 31 0 45 137 206 298 1.57e-39 139

Find 71 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAGGGTTTGGAGATTT+TGG 0.152777 4:+15078429 MsG0480019151.01.T01:five_prime_UTR
AACAATGCACCCGTTAATTA+AGG 0.239691 4:-15077187 None:intergenic
CCAGACGATGATGATGTTAA+TGG 0.273519 4:+15078705 MsG0480019151.01.T01:CDS
GGTTTGGTCACTTTCATCTT+TGG 0.293434 4:+15080562 MsG0480019151.01.T01:CDS
CCCGTCAGTCCCCATCCTTC+TGG 0.315780 4:-15079091 None:intergenic
TTCCTTTGTATATCATAATC+AGG 0.321016 4:-15080148 None:intergenic
GCTTGGAAAGGCACTAATCT+AGG 0.344675 4:+15080118 MsG0480019151.01.T01:CDS
TAACAAAATCCTTAATTAAC+GGG 0.374288 4:+15077178 None:intergenic
AACCCTCGAAGGACCGTAAT+CGG 0.374410 4:+15078998 MsG0480019151.01.T01:CDS
GTAACATATGGTGGTATAGA+TGG 0.416298 4:+15079045 MsG0480019151.01.T01:CDS
AGTCCCCATCCTTCTGGTTC+TGG 0.416906 4:-15079085 None:intergenic
GAGCAAGATAGAAGAGAAAG+AGG 0.419817 4:+15080584 MsG0480019151.01.T01:CDS
GAAAAGACAAATTCCGATTA+CGG 0.422754 4:-15079011 None:intergenic
CAGACTAGCAAAGTAACATA+TGG 0.425363 4:+15079033 MsG0480019151.01.T01:CDS
GAAGAACAAAGAAAGTTGTT+AGG 0.437376 4:+15080645 MsG0480019151.01.T01:CDS
TATCGTTACAAACCTTATCA+TGG 0.443589 4:-15079278 None:intergenic
TTAACAAAATCCTTAATTAA+CGG 0.444215 4:+15077177 None:intergenic
AAAGAAGCTGACACGACAAC+AGG 0.446251 4:+15079225 MsG0480019151.01.T01:CDS
GGATTCCTCAAGCTGTCAAA+CGG 0.446584 4:-15078533 None:intergenic
CCAGAAGGATGGGGACTGAC+GGG 0.454236 4:+15079091 MsG0480019151.01.T01:CDS
AGCATCAATATTCACACAAA+GGG 0.465791 4:-15080194 None:intergenic
TGAGAACACAAGCAGGGAAA+AGG 0.473043 4:+15078589 MsG0480019151.01.T01:five_prime_UTR
AATATTGCAGATTCTAGAGT+TGG 0.476430 4:+15080521 MsG0480019151.01.T01:intron
GATTCCTCAAGCTGTCAAAC+GGG 0.487934 4:-15078532 None:intergenic
CCATTAACATCATCATCGTC+TGG 0.487955 4:-15078705 None:intergenic
ATTCCGATTACGGTCCTTCG+AGG 0.488138 4:-15079001 None:intergenic
CTTGATTCAGACGGCAAGGT+TGG 0.488695 4:+15079945 MsG0480019151.01.T01:CDS
CACTAGAGGAATCCATGATA+AGG 0.488735 4:+15079266 MsG0480019151.01.T01:CDS
ACTGAATCAGGTGGCGATTG+AGG 0.489439 4:+15079197 MsG0480019151.01.T01:intron
AGATGAGCTTACACACTTTG+AGG 0.492378 4:+15080093 MsG0480019151.01.T01:intron
TTACACACTTTGAGGAAGCT+TGG 0.497309 4:+15080101 MsG0480019151.01.T01:CDS
TGTATATCATAATCAGGTAC+TGG 0.499045 4:-15080142 None:intergenic
TAATATGAATTTCAAATCGA+GGG 0.502613 4:-15080878 None:intergenic
TCTTCATCATCATCTATCTC+AGG 0.504641 4:+15077230 MsG0480019151.01.T01:five_prime_UTR
CTCACCCGTTTGACAGCTTG+AGG 0.506393 4:+15078528 MsG0480019151.01.T01:five_prime_UTR
GATCTAGATGAAGTAGAACT+TGG 0.510217 4:-15078554 None:intergenic
ACGCTGCACAATCTTAATGA+AGG 0.518496 4:+15079978 MsG0480019151.01.T01:CDS
TTAGATAAAGAATATGCAGA+AGG 0.529750 4:+15078377 MsG0480019151.01.T01:five_prime_UTR
TAGATAAAGAATATGCAGAA+GGG 0.534133 4:+15078378 MsG0480019151.01.T01:five_prime_UTR
ACCAGAAGGATGGGGACTGA+CGG 0.534899 4:+15079090 MsG0480019151.01.T01:CDS
TTCCGATTACGGTCCTTCGA+GGG 0.541657 4:-15079000 None:intergenic
CTAATATGAATTTCAAATCG+AGG 0.543324 4:-15080879 None:intergenic
CTTGGAAAGGCACTAATCTA+GGG 0.548298 4:+15080119 MsG0480019151.01.T01:CDS
TCTACCAGAACCAGAAGGAT+GGG 0.564337 4:+15079081 MsG0480019151.01.T01:CDS
TTGAGGAAGCTTGATTCAGA+CGG 0.567117 4:+15079936 MsG0480019151.01.T01:CDS
TTCTACCAGAACCAGAAGGA+TGG 0.573020 4:+15079080 MsG0480019151.01.T01:CDS
CACTTTGAGGAAGCTTGGAA+AGG 0.574132 4:+15080106 MsG0480019151.01.T01:CDS
AGAAGACTGAGAACACAAGC+AGG 0.574200 4:+15078582 MsG0480019151.01.T01:five_prime_UTR
TCGTCTGGATGCTCAACAGA+TGG 0.576041 4:-15078690 None:intergenic
AGTATGTAAAATGTAGAATG+AGG 0.580460 4:+15080718 MsG0480019151.01.T01:three_prime_UTR
TATGAAAGTCGAAACAAATG+TGG 0.593107 4:+15080620 MsG0480019151.01.T01:CDS
CAATATTGAGTGAATTGTGT+AGG 0.597629 4:+15080670 MsG0480019151.01.T01:exon
AGAATTAGACTCTGATGATG+AGG 0.598339 4:+15078624 MsG0480019151.01.T01:five_prime_UTR
AAGCATCAATATTCACACAA+AGG 0.601790 4:-15080195 None:intergenic
CTAGCAGAGAGGAAAGGCAG+TGG 0.608241 4:+15078940 MsG0480019151.01.T01:CDS
CAGAAGGATGGGGACTGACG+GGG 0.612887 4:+15079092 MsG0480019151.01.T01:CDS
GAAGCTTGATTCAGACGGCA+AGG 0.613499 4:+15079941 MsG0480019151.01.T01:CDS
TGAGTACTAGCAGAGAGGAA+AGG 0.624923 4:+15078934 MsG0480019151.01.T01:intron
AGCTGACACGACAACAGGCG+AGG 0.627903 4:+15079230 MsG0480019151.01.T01:CDS
GAATGAGGTGTATTGTTGTG+CGG 0.631430 4:+15080733 MsG0480019151.01.T01:three_prime_UTR
GAAAGTCGAAACAAATGTGG+AGG 0.633362 4:+15080623 MsG0480019151.01.T01:CDS
CTACCAGAACCAGAAGGATG+GGG 0.636130 4:+15079082 MsG0480019151.01.T01:CDS
TACCTGATTATGATATACAA+AGG 0.642517 4:+15080146 MsG0480019151.01.T01:CDS
ACTAGCAAAGTAACATATGG+TGG 0.655228 4:+15079036 MsG0480019151.01.T01:CDS
ATACTTCTACCAGAACCAGA+AGG 0.666727 4:+15079076 MsG0480019151.01.T01:CDS
GAAGACTGAGAACACAAGCA+GGG 0.671813 4:+15078583 MsG0480019151.01.T01:five_prime_UTR
ACACAAGCAGGGAAAAGGAG+TGG 0.685955 4:+15078594 MsG0480019151.01.T01:five_prime_UTR
CAAATCAGAAGAACCCTCGA+AGG 0.686390 4:+15078987 MsG0480019151.01.T01:CDS
GCAACACATAGAATCACTAG+AGG 0.698772 4:+15079252 MsG0480019151.01.T01:CDS
TCGTGTGAGTACTAGCAGAG+AGG 0.727899 4:+15078929 MsG0480019151.01.T01:intron
TGAGTGAATTGTGTAGGACA+TGG 0.729356 4:+15080676 MsG0480019151.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTATTAAAAATTTA+AGG - Chr4:15078180-15078199 None:intergenic 0.0%
!! AAAAAATGTCAAATTATGTA+TGG + Chr4:15079390-15079409 MsG0480019151.01.T01:intron 15.0%
!! AATTGTTTACTTCATTTAAA+TGG + Chr4:15080273-15080292 MsG0480019151.01.T01:intron 15.0%
!! ATAAATTGATATTTACGTAT+AGG - Chr4:15077379-15077398 None:intergenic 15.0%
!! ATTGTTTACTTCATTTAAAT+GGG + Chr4:15080274-15080293 MsG0480019151.01.T01:intron 15.0%
!! TTAAATAAAGATTGCAATAA+TGG + Chr4:15077526-15077545 MsG0480019151.01.T01:intron 15.0%
!! TTAAATGAAGTAAACAATTT+CGG - Chr4:15080272-15080291 None:intergenic 15.0%
!!! ACATTTTTTTGACAATTTTA+AGG - Chr4:15078007-15078026 None:intergenic 15.0%
!!! ATCTTATCATTATACTATTT+TGG + Chr4:15078776-15078795 MsG0480019151.01.T01:intron 15.0%
!!! GATTTTTATATCTAGTATTA+AGG - Chr4:15077432-15077451 None:intergenic 15.0%
!!! GTATTTTGAACTTATTTATT+TGG + Chr4:15078880-15078899 MsG0480019151.01.T01:intron 15.0%
!! AACAAAAAAATGATAGGAAT+CGG - Chr4:15079869-15079888 None:intergenic 20.0%
!! ACCTAAAACAAAAAAATGAT+AGG - Chr4:15079875-15079894 None:intergenic 20.0%
!! ACCTTCATAAAATAAAACTT+TGG + Chr4:15078086-15078105 MsG0480019151.01.T01:intron 20.0%
!! ATATCAAATATCTTGGATAA+AGG - Chr4:15077301-15077320 None:intergenic 20.0%
!! ATTGTGTTACAAATTAAGTT+AGG - Chr4:15080305-15080324 None:intergenic 20.0%
!! GAATTTGATATCAAATATCT+TGG - Chr4:15077308-15077327 None:intergenic 20.0%
!! GTTAATATTTAAGGAAATGA+AGG + Chr4:15079711-15079730 MsG0480019151.01.T01:intron 20.0%
!! TAATATGAATTTCAAATCGA+GGG - Chr4:15080881-15080900 None:intergenic 20.0%
!! TTAGTGTTTAAATACTTCAT+AGG + Chr4:15078255-15078274 MsG0480019151.01.T01:intron 20.0%
!! TTCTCTGTTATTGTATAATA+AGG + Chr4:15079841-15079860 MsG0480019151.01.T01:intron 20.0%
!! TTGAACTTATTTATTTGGTT+TGG + Chr4:15078885-15078904 MsG0480019151.01.T01:intron 20.0%
!!! AATTCTTATTTTCTTTCACT+TGG + Chr4:15077323-15077342 MsG0480019151.01.T01:intron 20.0%
!!! CAATAGAAGTTTTTTTATCA+CGG + Chr4:15078741-15078760 MsG0480019151.01.T01:intron 20.0%
!!! CTATTATATGTTTGTTTTCT+TGG + Chr4:15080792-15080811 MsG0480019151.01.T01:three_prime_UTR 20.0%
!!! GATAACAATGTCTTATTTAT+AGG - Chr4:15079813-15079832 None:intergenic 20.0%
!!! TATACACTATCTTCATTTTT+GGG + Chr4:15080382-15080401 MsG0480019151.01.T01:intron 20.0%
!!! TCCAAAGTTTTATTTTATGA+AGG - Chr4:15078090-15078109 None:intergenic 20.0%
!!! TCCTATCATTTTTTTGTTTT+AGG + Chr4:15079871-15079890 MsG0480019151.01.T01:intron 20.0%
!!! TTTTTTTGTTTTAGGTATTG+AGG + Chr4:15079879-15079898 MsG0480019151.01.T01:intron 20.0%
! AAAGAAAAACCTTCTACATT+AGG + Chr4:15079367-15079386 MsG0480019151.01.T01:intron 25.0%
! AAATAAAACTTTGGATGATG+TGG + Chr4:15078095-15078114 MsG0480019151.01.T01:intron 25.0%
! AACCAGGTTGTTAATATTTA+AGG + Chr4:15079702-15079721 MsG0480019151.01.T01:intron 25.0%
! AATGTAGAATCATCATTCTT+CGG + Chr4:15080455-15080474 MsG0480019151.01.T01:intron 25.0%
! ACAATAAGAAACTCAAATTG+AGG - Chr4:15080231-15080250 None:intergenic 25.0%
! AGTATGTAAAATGTAGAATG+AGG + Chr4:15080718-15080737 MsG0480019151.01.T01:three_prime_UTR 25.0%
! ATTGTACGATTGCAAAATAA+TGG + Chr4:15080246-15080265 MsG0480019151.01.T01:intron 25.0%
! CTAATATGAATTTCAAATCG+AGG - Chr4:15080882-15080901 None:intergenic 25.0%
! CTAGTTAGTTGATTAGAAAA+GGG + Chr4:15079751-15079770 MsG0480019151.01.T01:intron 25.0%
! CTATATGATATAACTGAATC+AGG + Chr4:15079185-15079204 MsG0480019151.01.T01:intron 25.0%
! GATTCTACATTAAACAAACA+TGG - Chr4:15080446-15080465 None:intergenic 25.0%
! GTGGTAAAAAGAAATAATGA+TGG + Chr4:15080047-15080066 MsG0480019151.01.T01:intron 25.0%
! TAAGTTTCTATATGTATGCA+GGG + Chr4:15079903-15079922 MsG0480019151.01.T01:intron 25.0%
! TACCTGATTATGATATACAA+AGG + Chr4:15080146-15080165 MsG0480019151.01.T01:CDS 25.0%
! TAGATAAAGAATATGCAGAA+GGG + Chr4:15078378-15078397 MsG0480019151.01.T01:five_prime_UTR 25.0%
! TCTAGTTAGTTGATTAGAAA+AGG + Chr4:15079750-15079769 MsG0480019151.01.T01:intron 25.0%
! TGATTAGAAAAGGGTTAATT+AGG + Chr4:15079760-15079779 MsG0480019151.01.T01:intron 25.0%
! TGTTAAGTGTAGATGAAATA+AGG + Chr4:15079341-15079360 MsG0480019151.01.T01:intron 25.0%
! TTAAGTTTCTATATGTATGC+AGG + Chr4:15079902-15079921 MsG0480019151.01.T01:intron 25.0%
! TTAGATAAAGAATATGCAGA+AGG + Chr4:15078377-15078396 MsG0480019151.01.T01:five_prime_UTR 25.0%
! TTCCTTAAATATTAACAACC+TGG - Chr4:15079707-15079726 None:intergenic 25.0%
! TTCCTTTGTATATCATAATC+AGG - Chr4:15080151-15080170 None:intergenic 25.0%
!! CTATACACTATCTTCATTTT+TGG + Chr4:15080381-15080400 MsG0480019151.01.T01:intron 25.0%
!! TGAAGATGAATTTTGAATAC+AGG + Chr4:15077399-15077418 MsG0480019151.01.T01:intron 25.0%
!! TTGCAAACATAAAGCTTTTA+AGG - Chr4:15077459-15077478 None:intergenic 25.0%
!!! ACATTTTTTCCTAATGTAGA+AGG - Chr4:15079379-15079398 None:intergenic 25.0%
!!! ATGGTTTGTTGTGTTTTATT+TGG + Chr4:15078330-15078349 MsG0480019151.01.T01:intron 25.0%
!!! TATTTGGTTTGGTTCATATA+TGG + Chr4:15078896-15078915 MsG0480019151.01.T01:intron 25.0%
!!! TTCTAAGTTTTTTGACATGA+TGG + Chr4:15078311-15078330 MsG0480019151.01.T01:intron 25.0%
AAGCATCAATATTCACACAA+AGG - Chr4:15080198-15080217 None:intergenic 30.0%
ACAATTAAATGTAACGTGTG+TGG - Chr4:15077621-15077640 None:intergenic 30.0%
ACGCTTTCAATATGAAATGT+TGG - Chr4:15077574-15077593 None:intergenic 30.0%
AGCATCAATATTCACACAAA+GGG - Chr4:15080197-15080216 None:intergenic 30.0%
AGCTAAACAAAAACAAGGTT+TGG + Chr4:15080546-15080565 MsG0480019151.01.T01:CDS 30.0%
ATAGAGATTGCAATATACGA+TGG + Chr4:15077487-15077506 MsG0480019151.01.T01:intron 30.0%
CAATATTGAGTGAATTGTGT+AGG + Chr4:15080670-15080689 MsG0480019151.01.T01:exon 30.0%
CACAAAGGGAACAAAAAAAA+AGG - Chr4:15080183-15080202 None:intergenic 30.0%
CATGTATGAAGATGATGAAA+TGG - Chr4:15077265-15077284 None:intergenic 30.0%
GAAAAGACAAATTCCGATTA+CGG - Chr4:15079014-15079033 None:intergenic 30.0%
GAAGAACAAAGAAAGTTGTT+AGG + Chr4:15080645-15080664 MsG0480019151.01.T01:CDS 30.0%
GAGCAATATATATAAGTGAG+TGG + Chr4:15077850-15077869 MsG0480019151.01.T01:intron 30.0%
GATAAGGTTTGTAACGATAT+AGG + Chr4:15079282-15079301 MsG0480019151.01.T01:intron 30.0%
GTTCAAAAGCTGTCATAAAA+TGG - Chr4:15077735-15077754 None:intergenic 30.0%
TAAATACTTCATAGGCTCAT+AGG + Chr4:15078263-15078282 MsG0480019151.01.T01:intron 30.0%
TAAGAAACTCAAATTGAGGA+AGG - Chr4:15080227-15080246 None:intergenic 30.0%
TATCGTTACAAACCTTATCA+TGG - Chr4:15079281-15079300 None:intergenic 30.0%
TATGAAAGTCGAAACAAATG+TGG + Chr4:15080620-15080639 MsG0480019151.01.T01:CDS 30.0%
TATGATATAACTGAATCAGG+TGG + Chr4:15079188-15079207 MsG0480019151.01.T01:intron 30.0%
TCAAATAAGCTAATTCAACC+AGG + Chr4:15079686-15079705 MsG0480019151.01.T01:intron 30.0%
TCATAGGCTGAATATCAAAA+GGG + Chr4:15078279-15078298 MsG0480019151.01.T01:intron 30.0%
TGTATATCATAATCAGGTAC+TGG - Chr4:15080145-15080164 None:intergenic 30.0%
TTGAGACAATTAACACAAAG+AGG - Chr4:15079140-15079159 None:intergenic 30.0%
! AAACTTAAGGCATTAGGTTA+TGG - Chr4:15077882-15077901 None:intergenic 30.0%
! AACTTAAGGCATTAGGTTAT+GGG - Chr4:15077881-15077900 None:intergenic 30.0%
! ACAGTGTTTTCAAATCAAGA+GGG + Chr4:15078415-15078434 MsG0480019151.01.T01:five_prime_UTR 30.0%
! AGTCTTGATGAGAAATTTTG+AGG + Chr4:15078655-15078674 MsG0480019151.01.T01:five_prime_UTR 30.0%
! TAACCTAATGCCTTAAGTTT+TGG + Chr4:15077882-15077901 MsG0480019151.01.T01:intron 30.0%
! TGGACTTGAAATTTTGCAAT+TGG + Chr4:15077343-15077362 MsG0480019151.01.T01:intron 30.0%
! TTTTATCACGGTTAATCGAT+CGG + Chr4:15078753-15078772 MsG0480019151.01.T01:intron 30.0%
! TTTTCATATGCTCTCCAAAA+CGG + Chr4:15079633-15079652 MsG0480019151.01.T01:intron 30.0%
!! AATATTGCAGATTCTAGAGT+TGG + Chr4:15080521-15080540 MsG0480019151.01.T01:intron 30.0%
!!! AGAAATTTTGAGGTCATCAA+TGG + Chr4:15078665-15078684 MsG0480019151.01.T01:exon 30.0%
AACAATGCACCCGTTAATTA+AGG - Chr4:15077190-15077209 None:intergenic 35.0%
ACTAGATTGGATCATCACTT+CGG - Chr4:15077950-15077969 None:intergenic 35.0%
ACTAGCAAAGTAACATATGG+TGG + Chr4:15079036-15079055 MsG0480019151.01.T01:CDS 35.0%
ATTGCAATATACGATGGTTG+TGG + Chr4:15077493-15077512 MsG0480019151.01.T01:intron 35.0%
CAGACTAGCAAAGTAACATA+TGG + Chr4:15079033-15079052 MsG0480019151.01.T01:CDS 35.0%
CAGATCTTCACCAAAACTTA+AGG - Chr4:15077895-15077914 None:intergenic 35.0%
CATCAGTTGCACTTCAAATT+CGG + Chr4:15078039-15078058 MsG0480019151.01.T01:intron 35.0%
CTCATAGGCTGAATATCAAA+AGG + Chr4:15078278-15078297 MsG0480019151.01.T01:intron 35.0%
GATCTAGATGAAGTAGAACT+TGG - Chr4:15078557-15078576 None:intergenic 35.0%
GTAACATATGGTGGTATAGA+TGG + Chr4:15079045-15079064 MsG0480019151.01.T01:CDS 35.0%
GTGAAAAAAGGATCGTCAAA+TGG - Chr4:15078509-15078528 None:intergenic 35.0%
TAACACACTAGTCAATCAAC+AGG - Chr4:15079457-15079476 None:intergenic 35.0%
TAATGGTTGTGTCCATAGTT+AGG + Chr4:15077543-15077562 MsG0480019151.01.T01:intron 35.0%
TAGGGTTGTTATAAGAGAAG+TGG + Chr4:15077698-15077717 MsG0480019151.01.T01:intron 35.0%
TATATGTTCAACAGTTGCTC+AGG + Chr4:15079427-15079446 MsG0480019151.01.T01:intron 35.0%
TCACTAGTGGTTATGTAAGT+AGG + Chr4:15079658-15079677 MsG0480019151.01.T01:intron 35.0%
TCTTCATCATCATCTATCTC+AGG + Chr4:15077230-15077249 MsG0480019151.01.T01:five_prime_UTR 35.0%
TGGTGAGCTAAACAAAAACA+AGG + Chr4:15080541-15080560 MsG0480019151.01.T01:CDS 35.0%
TTGTTTAACAACATCTCGAG+TGG + Chr4:15080028-15080047 MsG0480019151.01.T01:intron 35.0%
! AGAATTAGACTCTGATGATG+AGG + Chr4:15078624-15078643 MsG0480019151.01.T01:five_prime_UTR 35.0%
! AGGTAGAAGTATAACATGTG+TGG + Chr4:15077825-15077844 MsG0480019151.01.T01:intron 35.0%
! CTAGTGTGTTAGCTTCTATT+TGG + Chr4:15079466-15079485 MsG0480019151.01.T01:intron 35.0%
! GACAGTGTTTTCAAATCAAG+AGG + Chr4:15078414-15078433 MsG0480019151.01.T01:five_prime_UTR 35.0%
! GTTTTCAAATCAAGAGGGTT+TGG + Chr4:15078420-15078439 MsG0480019151.01.T01:five_prime_UTR 35.0%
! TCACCAAAACTTAAGGCATT+AGG - Chr4:15077888-15077907 None:intergenic 35.0%
! TTTGCTTGACAAGTTCTGAT+TGG + Chr4:15078821-15078840 MsG0480019151.01.T01:intron 35.0%
!! CTTAAGTTTTGGTGAAGATC+TGG + Chr4:15077893-15077912 MsG0480019151.01.T01:intron 35.0%
!!! TTTGGAGATTTTGGTGGATT+TGG + Chr4:15078438-15078457 MsG0480019151.01.T01:five_prime_UTR 35.0%
!!! TTTTTGGGTGCAACTTAGAT+TGG + Chr4:15080397-15080416 MsG0480019151.01.T01:intron 35.0%
AAGCTATCGTAGAGAGCTTA+TGG + Chr4:15079595-15079614 MsG0480019151.01.T01:intron 40.0%
ACGCTGCACAATCTTAATGA+AGG + Chr4:15079978-15079997 MsG0480019151.01.T01:CDS 40.0%
AGATGAGCTTACACACTTTG+AGG + Chr4:15080093-15080112 MsG0480019151.01.T01:intron 40.0%
AGTGATGATCCAATCTAGTG+AGG + Chr4:15077951-15077970 MsG0480019151.01.T01:intron 40.0%
ATACTTCTACCAGAACCAGA+AGG + Chr4:15079076-15079095 MsG0480019151.01.T01:CDS 40.0%
ATCTCTCCTCCAAAAAGACT+AGG - Chr4:15078486-15078505 None:intergenic 40.0%
ATGTTGGAGCTACCTAACTA+TGG - Chr4:15077558-15077577 None:intergenic 40.0%
CACTAGAGGAATCCATGATA+AGG + Chr4:15079266-15079285 MsG0480019151.01.T01:CDS 40.0%
CCATTAACATCATCATCGTC+TGG - Chr4:15078708-15078727 None:intergenic 40.0%
GAAAGTCGAAACAAATGTGG+AGG + Chr4:15080623-15080642 MsG0480019151.01.T01:CDS 40.0%
GAATGAGGTGTATTGTTGTG+CGG + Chr4:15080733-15080752 MsG0480019151.01.T01:three_prime_UTR 40.0%
GAGCAAGATAGAAGAGAAAG+AGG + Chr4:15080584-15080603 MsG0480019151.01.T01:CDS 40.0%
GCAACACATAGAATCACTAG+AGG + Chr4:15079252-15079271 MsG0480019151.01.T01:CDS 40.0%
GGAAATGAAGGATCTGTGAT+CGG + Chr4:15079723-15079742 MsG0480019151.01.T01:intron 40.0%
TCAAACGGGTGAGTGAAAAA+AGG - Chr4:15078521-15078540 None:intergenic 40.0%
TGAGTGAATTGTGTAGGACA+TGG + Chr4:15080676-15080695 MsG0480019151.01.T01:exon 40.0%
TTAAGGATTGTGATCCTGCT+GGG - Chr4:15077990-15078009 None:intergenic 40.0%
TTGAGGAAGCTTGATTCAGA+CGG + Chr4:15079936-15079955 MsG0480019151.01.T01:CDS 40.0%
TTTAAGGATTGTGATCCTGC+TGG - Chr4:15077991-15078010 None:intergenic 40.0%
! CCAGACGATGATGATGTTAA+TGG + Chr4:15078705-15078724 MsG0480019151.01.T01:CDS 40.0%
! GGTTTGGTCACTTTCATCTT+TGG + Chr4:15080562-15080581 MsG0480019151.01.T01:CDS 40.0%
! TATTGAAAGCGTATCGAGTG+TGG + Chr4:15077582-15077601 MsG0480019151.01.T01:intron 40.0%
! TCAAGAGGGTTTGGAGATTT+TGG + Chr4:15078429-15078448 MsG0480019151.01.T01:five_prime_UTR 40.0%
! TGGTCTCTAACTTGCATATG+TGG + Chr4:15077913-15077932 MsG0480019151.01.T01:intron 40.0%
! TTACACACTTTGAGGAAGCT+TGG + Chr4:15080101-15080120 MsG0480019151.01.T01:CDS 40.0%
!! ATGACGATGCCTAGTCTTTT+TGG + Chr4:15078474-15078493 MsG0480019151.01.T01:five_prime_UTR 40.0%
!! CTTGGAAAGGCACTAATCTA+GGG + Chr4:15080119-15080138 MsG0480019151.01.T01:CDS 40.0%
!! TAGGCATCGTCATACTTTTG+TGG - Chr4:15078467-15078486 None:intergenic 40.0%
AAAGAAGCTGACACGACAAC+AGG + Chr4:15079225-15079244 MsG0480019151.01.T01:CDS 45.0%
AGAAGACTGAGAACACAAGC+AGG + Chr4:15078582-15078601 MsG0480019151.01.T01:five_prime_UTR 45.0%
CAAATCAGAAGAACCCTCGA+AGG + Chr4:15078987-15079006 MsG0480019151.01.T01:CDS 45.0%
GAAGACTGAGAACACAAGCA+GGG + Chr4:15078583-15078602 MsG0480019151.01.T01:five_prime_UTR 45.0%
GATTCCTCAAGCTGTCAAAC+GGG - Chr4:15078535-15078554 None:intergenic 45.0%
GGATTCCTCAAGCTGTCAAA+CGG - Chr4:15078536-15078555 None:intergenic 45.0%
TAACACAAAGAGGAAGTCGC+AGG - Chr4:15079130-15079149 None:intergenic 45.0%
TCTACCAGAACCAGAAGGAT+GGG + Chr4:15079081-15079100 MsG0480019151.01.T01:CDS 45.0%
TGAGAACACAAGCAGGGAAA+AGG + Chr4:15078589-15078608 MsG0480019151.01.T01:five_prime_UTR 45.0%
TGAGTACTAGCAGAGAGGAA+AGG + Chr4:15078934-15078953 MsG0480019151.01.T01:intron 45.0%
TTCTACCAGAACCAGAAGGA+TGG + Chr4:15079080-15079099 MsG0480019151.01.T01:CDS 45.0%
! AACCACTAGTGAGACCGTTT+TGG - Chr4:15079650-15079669 None:intergenic 45.0%
! AGAGGGTTTGGAGATTTTGG+TGG + Chr4:15078432-15078451 MsG0480019151.01.T01:five_prime_UTR 45.0%
! CACTTTGAGGAAGCTTGGAA+AGG + Chr4:15080106-15080125 MsG0480019151.01.T01:CDS 45.0%
! CGGGGTGTCTATGTTTCATA+GGG + Chr4:15077680-15077699 MsG0480019151.01.T01:intron 45.0%
! TCGGGGTGTCTATGTTTCAT+AGG + Chr4:15077679-15077698 MsG0480019151.01.T01:intron 45.0%
! TGCAGGGTCATTCAGTTTTG+AGG + Chr4:15079919-15079938 MsG0480019151.01.T01:intron 45.0%
!! ACGATGCCTAGTCTTTTTGG+AGG + Chr4:15078477-15078496 MsG0480019151.01.T01:five_prime_UTR 45.0%
!! GCTTGGAAAGGCACTAATCT+AGG + Chr4:15080118-15080137 MsG0480019151.01.T01:CDS 45.0%
!!! ATTTTTTTTTTCAAATATTA+AGG + Chr4:15077644-15077663 MsG0480019151.01.T01:intron 5.0%
!!! TTTTTAATAAAAATATAATG+TGG + Chr4:15078184-15078203 MsG0480019151.01.T01:intron 5.0%
!!! TTTTTTTTTTCAAATATTAA+GGG + Chr4:15077645-15077664 MsG0480019151.01.T01:intron 5.0%
AAAGCGTATCGAGTGTGGAG+TGG + Chr4:15077587-15077606 MsG0480019151.01.T01:intron 50.0%
AACCCTCGAAGGACCGTAAT+CGG + Chr4:15078998-15079017 MsG0480019151.01.T01:CDS 50.0%
ACACAAGCAGGGAAAAGGAG+TGG + Chr4:15078594-15078613 MsG0480019151.01.T01:five_prime_UTR 50.0%
ACGACTGTGTAGCAAGTCTG+AGG + Chr4:15077805-15077824 MsG0480019151.01.T01:intron 50.0%
ACTGAATCAGGTGGCGATTG+AGG + Chr4:15079197-15079216 MsG0480019151.01.T01:intron 50.0%
ATAGACACCCCGACATGACA+CGG - Chr4:15077672-15077691 None:intergenic 50.0%
ATTAAGGGCCCGTGTCATGT+CGG + Chr4:15077660-15077679 MsG0480019151.01.T01:intron 50.0%
ATTCCGATTACGGTCCTTCG+AGG - Chr4:15079004-15079023 None:intergenic 50.0%
CCAAGAACGCATTTCTGCAG+TGG + Chr4:15079306-15079325 MsG0480019151.01.T01:intron 50.0%
CCACTGCAGAAATGCGTTCT+TGG - Chr4:15079309-15079328 None:intergenic 50.0%
CTACCAGAACCAGAAGGATG+GGG + Chr4:15079082-15079101 MsG0480019151.01.T01:CDS 50.0%
CTCCAAAACGGTCTCACTAG+TGG + Chr4:15079645-15079664 MsG0480019151.01.T01:intron 50.0%
CTTGATTCAGACGGCAAGGT+TGG + Chr4:15079945-15079964 MsG0480019151.01.T01:CDS 50.0%
GAAGCTTGATTCAGACGGCA+AGG + Chr4:15079941-15079960 MsG0480019151.01.T01:CDS 50.0%
TCGTCTGGATGCTCAACAGA+TGG - Chr4:15078693-15078712 None:intergenic 50.0%
TCGTGTGAGTACTAGCAGAG+AGG + Chr4:15078929-15078948 MsG0480019151.01.T01:intron 50.0%
TTCCGATTACGGTCCTTCGA+GGG - Chr4:15079003-15079022 None:intergenic 50.0%
ACCAGAAGGATGGGGACTGA+CGG + Chr4:15079090-15079109 MsG0480019151.01.T01:CDS 55.0%
CTAGCAGAGAGGAAAGGCAG+TGG + Chr4:15078940-15078959 MsG0480019151.01.T01:CDS 55.0%
CTCACCCGTTTGACAGCTTG+AGG + Chr4:15078528-15078547 MsG0480019151.01.T01:five_prime_UTR 55.0%
TAGACACCCCGACATGACAC+GGG - Chr4:15077671-15077690 None:intergenic 55.0%
TTAAGGGCCCGTGTCATGTC+GGG + Chr4:15077661-15077680 MsG0480019151.01.T01:intron 55.0%
!! AGTCCCCATCCTTCTGGTTC+TGG - Chr4:15079088-15079107 None:intergenic 55.0%
AGCTGACACGACAACAGGCG+AGG + Chr4:15079230-15079249 MsG0480019151.01.T01:CDS 60.0%
CAGAAGGATGGGGACTGACG+GGG + Chr4:15079092-15079111 MsG0480019151.01.T01:CDS 60.0%
CCAGAAGGATGGGGACTGAC+GGG + Chr4:15079091-15079110 MsG0480019151.01.T01:CDS 60.0%
GAGGGGGAGCCTCACTAGAT+TGG - Chr4:15077963-15077982 None:intergenic 60.0%
GTGATCCTGCTGGGCCATGA+GGG - Chr4:15077981-15078000 None:intergenic 60.0%
TAAGGGCCCGTGTCATGTCG+GGG + Chr4:15077662-15077681 MsG0480019151.01.T01:intron 60.0%
TCTAGTGAGGCTCCCCCTCA+TGG + Chr4:15077964-15077983 MsG0480019151.01.T01:intron 60.0%
TGATCCTGCTGGGCCATGAG+GGG - Chr4:15077980-15077999 None:intergenic 60.0%
TGTGATCCTGCTGGGCCATG+AGG - Chr4:15077982-15078001 None:intergenic 60.0%
CCCGTCAGTCCCCATCCTTC+TGG - Chr4:15079094-15079113 None:intergenic 65.0%
GATCCTGCTGGGCCATGAGG+GGG - Chr4:15077979-15077998 None:intergenic 65.0%
GCTCCCCCTCATGGCCCAGC+AGG + Chr4:15077973-15077992 MsG0480019151.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 15077179 15080901 15077179 ID=MsG0480019151.01;Name=MsG0480019151.01
Chr4 mRNA 15077179 15080901 15077179 ID=MsG0480019151.01.T01;Parent=MsG0480019151.01;Name=MsG0480019151.01.T01;_AED=0.49;_eAED=0.50;_QI=381|0.66|0.71|1|0.66|0.57|7|219|216
Chr4 exon 15077179 15077251 15077179 ID=MsG0480019151.01.T01:exon:4054;Parent=MsG0480019151.01.T01
Chr4 exon 15078376 15078726 15078376 ID=MsG0480019151.01.T01:exon:4055;Parent=MsG0480019151.01.T01
Chr4 exon 15078938 15079113 15078938 ID=MsG0480019151.01.T01:exon:4056;Parent=MsG0480019151.01.T01
Chr4 exon 15079207 15079287 15079207 ID=MsG0480019151.01.T01:exon:4057;Parent=MsG0480019151.01.T01
Chr4 exon 15079924 15079999 15079924 ID=MsG0480019151.01.T01:exon:4058;Parent=MsG0480019151.01.T01
Chr4 exon 15080095 15080217 15080095 ID=MsG0480019151.01.T01:exon:4059;Parent=MsG0480019151.01.T01
Chr4 exon 15080531 15080901 15080531 ID=MsG0480019151.01.T01:exon:4060;Parent=MsG0480019151.01.T01
Chr4 five_prime_UTR 15077179 15077251 15077179 ID=MsG0480019151.01.T01:five_prime_utr;Parent=MsG0480019151.01.T01
Chr4 five_prime_UTR 15078376 15078683 15078376 ID=MsG0480019151.01.T01:five_prime_utr;Parent=MsG0480019151.01.T01
Chr4 CDS 15078684 15078726 15078684 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 CDS 15078938 15079113 15078938 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 CDS 15079207 15079287 15079207 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 CDS 15079924 15079999 15079924 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 CDS 15080095 15080217 15080095 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 CDS 15080531 15080682 15080531 ID=MsG0480019151.01.T01:cds;Parent=MsG0480019151.01.T01
Chr4 three_prime_UTR 15080683 15080901 15080683 ID=MsG0480019151.01.T01:three_prime_utr;Parent=MsG0480019151.01.T01
Gene Sequence

>MsG0480019151.01.T01

ATGGAGCCATCTGTTGAGCATCCAGACGATGATGATGTTAATGTACTAGCAGAGAGGAAAGGCAGTGGCATGACTCACAAGATTGATCTTCACAAATCAGAAGAACCCTCGAAGGACCGTAATCGGAATTTGTCTTTTCAGACTAGCAAAGTAACATATGGTGGTATAGATGGTGCTTACTATACTTCTACCAGAACCAGAAGGATGGGGACTGACGGGGTGGCGATTGAGGAAAGCAAAGAAGCTGACACGACAACAGGCGAGGCAACACATAGAATCACTAGAGGAATCCATGATAAGGGTCATTCAGTTTTGAGGAAGCTTGATTCAGACGGCAAGGTTGGTACAACACAAACGCTGCACAATCTTAATGAAGATGAGCTTACACACTTTGAGGAAGCTTGGAAAGGCACTAATCTAGGGCCAGTACCTGATTATGATATACAAAGGAAGAAAGTTAGCCTTTTTTTTTGTTCCCTTTGTGTGAATATTGATGCTTATTCTAGAGTTGGTGAGCTAAACAAAAACAAGGTTTGGTCACTTTCATCTTTGGAGCAAGATAGAAGAGAAAGAGGATTTTCATCAAATTATGAAAGTCGAAACAAATGTGGAGGAAGAACAAAGAAAGTTGTTAGGATCAATATTGAGTGA
Protein sequence

>MsG0480019151.01.T01

MEPSVEHPDDDDVNVLAERKGSGMTHKIDLHKSEEPSKDRNRNLSFQTSKVTYGGIDGAYYTSTRTRRMGTDGVAIEESKEADTTTGEATHRITRGIHDKGHSVLRKLDSDGKVGTTQTLHNLNEDELTHFEEAWKGTNLGPVPDYDIQRKKVSLFFCSLCVNIDAYSRVGELNKNKVWSLSSLEQDRRERGFSSNYESRNKCGGRTKKVVRINIE*