AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019474.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019474.01.T01 MTR_8g083140 82.544 338 28 7 3 309 99 436 3.54e-174 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019474.01.T01 AT4G08580 61.039 308 85 8 36 308 128 435 5.30e-113 334
MsG0480019474.01.T01 AT4G08580 61.039 308 85 8 36 308 128 435 5.30e-113 334
MsG0480019474.01.T01 AT5G17900 66.160 263 70 7 65 308 173 435 1.36e-112 333

Find 102 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGAAAGCACTTCATTAT+TGG 0.237393 4:-20824739 MsG0480019474.01.T01:three_prime_UTR
AAACATCAATGAATCGTTAT+TGG 0.259097 4:-20826771 None:intergenic
CGCGATTTCTCTGCACCAAC+TGG 0.272436 4:-20826082 MsG0480019474.01.T01:CDS
GCTGTTGATAAGGATTATAT+TGG 0.298227 4:-20824567 MsG0480019474.01.T01:three_prime_UTR
CATCACAATTACATTGTTAC+TGG 0.299186 4:-20824589 MsG0480019474.01.T01:three_prime_UTR
GGAGAGAAGAAGACGGATTA+AGG 0.319392 4:-20826662 MsG0480019474.01.T01:CDS
TTTGAGGATTGAGAAGGTTT+TGG 0.325987 4:+20826208 None:intergenic
ACCAACTGGGGAAGATAAAA+TGG 0.335988 4:-20826068 MsG0480019474.01.T01:CDS
AAACCTCCATTTCTGCTTTG+AGG 0.348536 4:+20826192 None:intergenic
TGCCATTCTCTCATTATACT+TGG 0.351558 4:+20824843 None:intergenic
ATTGTTACTGGCTGTTGATA+AGG 0.364885 4:-20824577 MsG0480019474.01.T01:three_prime_UTR
TCACTTAATGAGTGAATCAT+AGG 0.368074 4:-20824636 MsG0480019474.01.T01:three_prime_UTR
ATGGTATCTCTCTCCGACTT+TGG 0.379028 4:+20826538 None:intergenic
CTGTTGATAAGGATTATATT+GGG 0.399018 4:-20824566 MsG0480019474.01.T01:three_prime_UTR
CTCAATCCTCAAAGCAGAAA+TGG 0.400415 4:-20826198 MsG0480019474.01.T01:CDS
AAGAAAAGGAAGAGCTTGAG+AGG 0.413111 4:-20826264 MsG0480019474.01.T01:CDS
AAAACGAGTAGCATCAATAT+AGG 0.425653 4:+20824539 None:intergenic
AGCGAAGCGCAGAAAATTGA+AGG 0.430815 4:-20824803 MsG0480019474.01.T01:CDS
AGAACAAGATGAGGAGTCTA+TGG 0.433939 4:-20826686 MsG0480019474.01.T01:CDS
GGCGAAGAGGCAGGAAGGGT+TGG 0.434799 4:-20826716 MsG0480019474.01.T01:CDS
AATATGAAGCTTGGAAGAAA+AGG 0.436853 4:-20826324 MsG0480019474.01.T01:CDS
GGAAGCAAGGAAGAGGAGAA+TGG 0.439115 4:-20826479 MsG0480019474.01.T01:CDS
AGGTTGTTGTTGAGGAGATT+CGG 0.445717 4:-20826432 MsG0480019474.01.T01:CDS
TGGCTTAAGCATGCATGCTA+TGG 0.448743 4:-20824719 MsG0480019474.01.T01:three_prime_UTR
AGGAAGAGCTTGAGAGGGTG+AGG 0.449581 4:-20826258 MsG0480019474.01.T01:CDS
CAAGCGGGATAGGGAGGAGC+GGG 0.468410 4:-20826299 MsG0480019474.01.T01:CDS
AGAAAAGGATCAAGCGGGAT+AGG 0.473338 4:-20826309 MsG0480019474.01.T01:CDS
AGCTGAAGAGGAGGCTCTTG+AGG 0.477467 4:-20826500 MsG0480019474.01.T01:CDS
AGCGCAGAAAATTGAAGGAC+TGG 0.478978 4:-20824798 MsG0480019474.01.T01:CDS
TGTTTGACTTGCATAACCTT+GGG 0.486510 4:+20826031 None:intergenic
AGCATGCATGCTATGGATCA+TGG 0.488074 4:-20824712 MsG0480019474.01.T01:three_prime_UTR
GGAATATGACAGAGGAGGAA+AGG 0.492468 4:-20826237 MsG0480019474.01.T01:CDS
TGAAGAGGAGGCGAAGAGGC+AGG 0.494479 4:-20826725 MsG0480019474.01.T01:CDS
CTCGGTCATCGGAATTAGAC+TGG 0.496687 4:+20826137 None:intergenic
GAAGTTGCGTCAGAGAGATC+AGG 0.497425 4:-20826638 MsG0480019474.01.T01:CDS
AAGAACCAAGTGGTCCCATC+TGG 0.503765 4:-20825993 MsG0480019474.01.T01:CDS
ATCAATGAATCGTTATTGGC+AGG 0.505209 4:-20826767 None:intergenic
GAAAAGGATCAAGCGGGATA+GGG 0.505570 4:-20826308 MsG0480019474.01.T01:CDS
GTGTTTGACTTGCATAACCT+TGG 0.506919 4:+20826030 None:intergenic
GGAGCACGGTGTATGCAGAT+TGG 0.510074 4:-20824691 MsG0480019474.01.T01:three_prime_UTR
GGAGTTAGAAGAACAAGATG+AGG 0.514774 4:-20826695 MsG0480019474.01.T01:CDS
CTCTTGAGGAAGCAAGGAAG+AGG 0.518804 4:-20826486 MsG0480019474.01.T01:CDS
GAGAAGAAATGAGACGAGGC+AGG 0.523794 4:-20826452 MsG0480019474.01.T01:CDS
GGAGGCACTGTTGAAAGAAA+AGG 0.526980 4:-20826278 MsG0480019474.01.T01:CDS
GCGGGATAGGGAGGAGCGGG+AGG 0.531069 4:-20826296 MsG0480019474.01.T01:CDS
GCGATTTCTCTGCACCAACT+GGG 0.531801 4:-20826081 MsG0480019474.01.T01:CDS
AACAGTGGCAGCTCGGTCAT+CGG 0.532635 4:+20826126 None:intergenic
GAGGAGGCGAAGAGGCAGGA+AGG 0.535811 4:-20826721 MsG0480019474.01.T01:CDS
GACGAGGCAGGTTGTTGTTG+AGG 0.538406 4:-20826440 MsG0480019474.01.T01:CDS
GATGACCGAGCTGCCACTGT+TGG 0.538745 4:-20826124 MsG0480019474.01.T01:CDS
TCAATGAGGACACTACTGAT+TGG 0.545194 4:-20825970 MsG0480019474.01.T01:CDS
GCGCAGAAAATTGAAGGACT+GGG 0.548415 4:-20824797 MsG0480019474.01.T01:CDS
AGGAGGCTCTTGAGGAAGCA+AGG 0.551309 4:-20826492 MsG0480019474.01.T01:CDS
AGGCAGAGGAATATGAAGCT+TGG 0.552502 4:-20826333 MsG0480019474.01.T01:CDS
AGCAAGGAAGAGGAGAATGG+AGG 0.552786 4:-20826476 MsG0480019474.01.T01:CDS
TGATGAACTCAACGAGGCAG+AGG 0.554539 4:-20826347 MsG0480019474.01.T01:CDS
GTTATGCAAGTCAAACACTT+TGG 0.556744 4:-20826025 MsG0480019474.01.T01:CDS
TTCTGCTTTGAGGATTGAGA+AGG 0.556899 4:+20826202 None:intergenic
AGCCAAGTATAATGAGAGAA+TGG 0.558111 4:-20824845 MsG0480019474.01.T01:CDS
TAGTGTCCTCATTGACCAGA+TGG 0.558983 4:+20825978 None:intergenic
TGTTTCTACTATTGAAGAGG+AGG 0.560892 4:-20826737 MsG0480019474.01.T01:CDS
AGGAGGCGAAGAGGCAGGAA+GGG 0.561535 4:-20826720 MsG0480019474.01.T01:CDS
AGATCAGGAGGAAGAAGAAG+AGG 0.561768 4:-20826623 MsG0480019474.01.T01:CDS
CAGTTCCAACAGTGGCAGCT+CGG 0.562006 4:+20826119 None:intergenic
AGAAAAGGAAGAGCTTGAGA+GGG 0.564244 4:-20826263 MsG0480019474.01.T01:CDS
TATGGTGTTTGTTCCAAAGT+CGG 0.565511 4:-20826551 MsG0480019474.01.T01:CDS
GGAGGAAGAAGAAGAGGACG+AGG 0.570183 4:-20826617 MsG0480019474.01.T01:CDS
ATTGACCAGATGGGACCACT+TGG 0.576548 4:+20825988 None:intergenic
GTCAAACACTTTGGTCGCAG+TGG 0.580012 4:-20826016 MsG0480019474.01.T01:CDS
AGCTTCGAGACGCTCAGCAA+TGG 0.580307 4:+20826519 None:intergenic
AAGGATCAAGCGGGATAGGG+AGG 0.580990 4:-20826305 MsG0480019474.01.T01:CDS
TGAGTGCACTGGAGTAGCTA+TGG 0.582736 4:-20826569 MsG0480019474.01.T01:CDS
GATTGTTTCTACTATTGAAG+AGG 0.583283 4:-20826740 MsG0480019474.01.T01:CDS
GGTGAGGAATATGACAGAGG+AGG 0.583430 4:-20826242 MsG0480019474.01.T01:CDS
ACTGATTCTGATGAGTGCAC+TGG 0.585793 4:-20826580 MsG0480019474.01.T01:CDS
TGAGCGTCTCGAAGCTGAAG+AGG 0.590653 4:-20826512 MsG0480019474.01.T01:CDS
AAAGAATAAACAGATGAACT+AGG 0.598195 4:+20824660 None:intergenic
GAGGGTGAGGAATATGACAG+AGG 0.598749 4:-20826245 MsG0480019474.01.T01:CDS
CGATTTCTCTGCACCAACTG+GGG 0.600188 4:-20826080 MsG0480019474.01.T01:CDS
TGTTGTTGAGGAGATTCGGA+AGG 0.605323 4:-20826428 MsG0480019474.01.T01:CDS
TGTAATTGTGATGACATACA+AGG 0.611543 4:+20824599 None:intergenic
TTGGAAGAAAAGGATCAAGC+GGG 0.622672 4:-20826314 MsG0480019474.01.T01:CDS
CTTGGAAGAAAAGGATCAAG+CGG 0.624009 4:-20826315 MsG0480019474.01.T01:CDS
GGACAAGACAATATTGCCCA+AGG 0.627313 4:-20826047 MsG0480019474.01.T01:CDS
GTTGCGTCAGAGAGATCAGG+AGG 0.629878 4:-20826635 MsG0480019474.01.T01:CDS
TCAAGCGGGATAGGGAGGAG+CGG 0.631363 4:-20826300 MsG0480019474.01.T01:CDS
AGTGTCCTCATTGACCAGAT+GGG 0.631636 4:+20825979 None:intergenic
AATATTATCAGTTCCAACAG+TGG 0.632023 4:+20826111 None:intergenic
CTATTGAAGAGGAGGCGAAG+AGG 0.635858 4:-20826729 MsG0480019474.01.T01:CDS
GTGGTCCCATCTGGTCAATG+AGG 0.639359 4:-20825984 MsG0480019474.01.T01:CDS
GCGTCTCGAAGCTGAAGAGG+AGG 0.640835 4:-20826509 MsG0480019474.01.T01:CDS
AATCCTCAAAGCAGAAATGG+AGG 0.652484 4:-20826195 MsG0480019474.01.T01:CDS
ACAAGATGAGGAGTCTATGG+CGG 0.681651 4:-20826683 MsG0480019474.01.T01:CDS
CTATGGCGGAGAGAAGAAGA+CGG 0.681661 4:-20826669 MsG0480019474.01.T01:CDS
GTAATTGTGATGACATACAA+GGG 0.683518 4:+20824600 None:intergenic
TATGCAGAAATACTATCACA+AGG 0.688789 4:-20826170 MsG0480019474.01.T01:CDS
CACTGATGATGAACTCAACG+AGG 0.706626 4:-20826353 MsG0480019474.01.T01:CDS
ATGCAGAAATACTATCACAA+GGG 0.716221 4:-20826169 MsG0480019474.01.T01:CDS
AGGAGAGAAGAAATGAGACG+AGG 0.721838 4:-20826456 MsG0480019474.01.T01:CDS
ATTGTGATGACATACAAGGG+AGG 0.727011 4:+20824603 None:intergenic
ATGCTATGGATCATGGAGCA+CGG 0.740818 4:-20824705 MsG0480019474.01.T01:three_prime_UTR
GTCGCAGTGGAAGAACCAAG+TGG 0.754334 4:-20826003 MsG0480019474.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAATTAATATAATGATTA+AGG - Chr4:20825773-20825792 MsG0480019474.01.T01:intron 10.0%
!!! GTTTATTTTTAAATATAATC+TGG - Chr4:20826067-20826086 MsG0480019474.01.T01:CDS 10.0%
!! AAAAAATAAATCAATAATCC+AGG + Chr4:20826346-20826365 None:intergenic 15.0%
!! AATTCAGAAAAATATTGAAT+TGG - Chr4:20824879-20824898 MsG0480019474.01.T01:intron 15.0%
!! ACTAAAAACTGTTTATAAAT+TGG + Chr4:20825898-20825917 None:intergenic 15.0%
!! ATATCTTCAATAATATATAG+CGG - Chr4:20825359-20825378 MsG0480019474.01.T01:intron 15.0%
!! ATATTATTGAAGATATACAA+AGG + Chr4:20825355-20825374 None:intergenic 15.0%
!! ATGCAATCTTAAAAATTAAA+AGG - Chr4:20826290-20826309 MsG0480019474.01.T01:CDS 15.0%
!! TAATATCAACTAATAGAAAA+TGG + Chr4:20825391-20825410 None:intergenic 15.0%
!! TATAATAAGATACATTGATA+AGG - Chr4:20825589-20825608 MsG0480019474.01.T01:intron 15.0%
!! TATTATTGAAGATATACAAA+GGG + Chr4:20825354-20825373 None:intergenic 15.0%
!! TGCAATCTTAAAAATTAAAA+GGG - Chr4:20826291-20826310 MsG0480019474.01.T01:CDS 15.0%
!!! TTTTAATTTTTAAGATTGCA+TGG + Chr4:20826291-20826310 None:intergenic 15.0%
!! ATATCTAAAAAATCTCCATA+AGG + Chr4:20826238-20826257 None:intergenic 20.0%
!! GCAATCTTAAAAATTAAAAG+GGG - Chr4:20826292-20826311 MsG0480019474.01.T01:CDS 20.0%
!! TATCTAAAAAATCTCCATAA+GGG + Chr4:20826237-20826256 None:intergenic 20.0%
! AAAAACAAATAAAACACACG+AGG + Chr4:20825702-20825721 None:intergenic 25.0%
! AAAGAATAAACAGATGAACT+AGG + Chr4:20826620-20826639 None:intergenic 25.0%
! AAATATGTTTATGTCCCTTA+TGG - Chr4:20826220-20826239 MsG0480019474.01.T01:CDS 25.0%
! ATAAAAACAAACAGGAACAT+AGG + Chr4:20825676-20825695 None:intergenic 25.0%
! TCAGAAAAATATTGAATTGG+AGG - Chr4:20824882-20824901 MsG0480019474.01.T01:intron 25.0%
!! AAAATTAAAAGGGGTTTTCT+AGG - Chr4:20826301-20826320 MsG0480019474.01.T01:CDS 25.0%
!! CTGTTGATAAGGATTATATT+GGG - Chr4:20826711-20826730 MsG0480019474.01.T01:CDS 25.0%
!! GATTTTATATTGAGAAGCTT+AGG - Chr4:20825538-20825557 MsG0480019474.01.T01:intron 25.0%
!! TCATCAATGAAACCTTTTAT+CGG - Chr4:20825840-20825859 MsG0480019474.01.T01:intron 25.0%
!!! AAATGAAAGCACTTCATTAT+TGG - Chr4:20826538-20826557 MsG0480019474.01.T01:CDS 25.0%
!!! TACTTGTGTTCTATTTTAAC+AGG - Chr4:20826515-20826534 MsG0480019474.01.T01:CDS 25.0%
AAAACGAGTAGCATCAATAT+AGG + Chr4:20826741-20826760 None:intergenic 30.0%
AAGTCGCTCAAATAACAATA+AGG + Chr4:20826162-20826181 None:intergenic 30.0%
AATATGAAGCTTGGAAGAAA+AGG - Chr4:20824953-20824972 MsG0480019474.01.T01:intron 30.0%
AATATTATCAGTTCCAACAG+TGG + Chr4:20825169-20825188 None:intergenic 30.0%
ATAATCTGGATTCACACATA+AGG - Chr4:20826081-20826100 MsG0480019474.01.T01:CDS 30.0%
ATCCAAAACAAACCGATAAA+AGG + Chr4:20825855-20825874 None:intergenic 30.0%
ATGATTAAGGTCTATTCTCT+TGG - Chr4:20825786-20825805 MsG0480019474.01.T01:intron 30.0%
ATGCAGAAATACTATCACAA+GGG - Chr4:20825108-20825127 MsG0480019474.01.T01:intron 30.0%
ATGTTCTACTCAAGTTCATA+TGG - Chr4:20825467-20825486 MsG0480019474.01.T01:intron 30.0%
CATCACAATTACATTGTTAC+TGG - Chr4:20826688-20826707 MsG0480019474.01.T01:CDS 30.0%
GATAAAGCTAGCATTTCATA+TGG + Chr4:20825822-20825841 None:intergenic 30.0%
GTAATTGTGATGACATACAA+GGG + Chr4:20826680-20826699 None:intergenic 30.0%
TATGCAGAAATACTATCACA+AGG - Chr4:20825107-20825126 MsG0480019474.01.T01:intron 30.0%
TCACTTAATGAGTGAATCAT+AGG - Chr4:20826641-20826660 MsG0480019474.01.T01:CDS 30.0%
TGTAATTGTGATGACATACA+AGG + Chr4:20826681-20826700 None:intergenic 30.0%
TTAGGTCTAAACTGAAATCA+GGG - Chr4:20825556-20825575 MsG0480019474.01.T01:intron 30.0%
TTATCATGCATTCTCATTGT+AGG - Chr4:20825932-20825951 MsG0480019474.01.T01:intron 30.0%
! AATAAGGTTAGTTAGCTAGA+TGG + Chr4:20826146-20826165 None:intergenic 30.0%
! GCTGTTGATAAGGATTATAT+TGG - Chr4:20826710-20826729 MsG0480019474.01.T01:CDS 30.0%
! TAAAGAGAGAAGCAATGTTT+TGG + Chr4:20825439-20825458 None:intergenic 30.0%
! TATTATGTCCAAGACCATTT+TGG + Chr4:20825505-20825524 None:intergenic 30.0%
!! GATTGTTTCTACTATTGAAG+AGG - Chr4:20824537-20824556 MsG0480019474.01.T01:three_prime_UTR 30.0%
!! TTAATACTGATTTGCAATGC+AGG - Chr4:20826386-20826405 MsG0480019474.01.T01:CDS 30.0%
!! TTTCAGACAAGAAAAGTACT+TGG + Chr4:20826203-20826222 None:intergenic 30.0%
!!! AACCTTTTATCGGTTTGTTT+TGG - Chr4:20825850-20825869 MsG0480019474.01.T01:intron 30.0%
ACAAAGGGAAAGTGAACTTA+CGG + Chr4:20825339-20825358 None:intergenic 35.0%
AGCCAAGTATAATGAGAGAA+TGG - Chr4:20826432-20826451 MsG0480019474.01.T01:CDS 35.0%
AGGAGACATCTTTCAAACAA+AGG + Chr4:20825656-20825675 None:intergenic 35.0%
ATACGAAGAAGACAACTAAG+TGG - Chr4:20825612-20825631 MsG0480019474.01.T01:intron 35.0%
CGAGGACTATAAAAACAAAC+AGG + Chr4:20825684-20825703 None:intergenic 35.0%
CTTAGGTCTAAACTGAAATC+AGG - Chr4:20825555-20825574 MsG0480019474.01.T01:intron 35.0%
GAGAATGCATGATAAAACTC+TGG + Chr4:20825927-20825946 None:intergenic 35.0%
GTTATGCAAGTCAAACACTT+TGG - Chr4:20825252-20825271 MsG0480019474.01.T01:intron 35.0%
TATGGTGTTTGTTCCAAAGT+CGG - Chr4:20824726-20824745 MsG0480019474.01.T01:three_prime_UTR 35.0%
TGCCATTCTCTCATTATACT+TGG + Chr4:20826437-20826456 None:intergenic 35.0%
TGGATATTGATGTAGTTTCG+TGG - Chr4:20825870-20825889 MsG0480019474.01.T01:intron 35.0%
! ATTGTTACTGGCTGTTGATA+AGG - Chr4:20826700-20826719 MsG0480019474.01.T01:CDS 35.0%
! TGCTAGGATGATTTTATACC+TGG - Chr4:20826325-20826344 MsG0480019474.01.T01:CDS 35.0%
! TGTTTCTACTATTGAAGAGG+AGG - Chr4:20824540-20824559 MsG0480019474.01.T01:three_prime_UTR 35.0%
! TTTGAGGATTGAGAAGGTTT+TGG + Chr4:20825072-20825091 None:intergenic 35.0%
!! CATTCTCATTGTAGGTTGAT+AGG - Chr4:20825940-20825959 MsG0480019474.01.T01:intron 35.0%
!! GAAACCAGCTTTAGCATTTT+AGG - Chr4:20825999-20826018 MsG0480019474.01.T01:CDS 35.0%
!! TGTTTGACTTGCATAACCTT+GGG + Chr4:20825249-20825268 None:intergenic 35.0%
!! TTCACACATAAGGTGGATTA+TGG - Chr4:20826091-20826110 MsG0480019474.01.T01:CDS 35.0%
!!! GTCTATTCTCTTGGTGTTTT+TGG - Chr4:20825795-20825814 MsG0480019474.01.T01:intron 35.0%
AAACCTCCATTTCTGCTTTG+AGG + Chr4:20825088-20825107 None:intergenic 40.0%
AAGAAAAGGAAGAGCTTGAG+AGG - Chr4:20825013-20825032 MsG0480019474.01.T01:intron 40.0%
AATCCTCAAAGCAGAAATGG+AGG - Chr4:20825082-20825101 MsG0480019474.01.T01:intron 40.0%
ACACTCATCCAAAATGGTCT+TGG - Chr4:20825494-20825513 MsG0480019474.01.T01:intron 40.0%
ACCAACTGGGGAAGATAAAA+TGG - Chr4:20825209-20825228 MsG0480019474.01.T01:intron 40.0%
AGAAAAGGAAGAGCTTGAGA+GGG - Chr4:20825014-20825033 MsG0480019474.01.T01:intron 40.0%
AGAACAAGATGAGGAGTCTA+TGG - Chr4:20824591-20824610 MsG0480019474.01.T01:three_prime_UTR 40.0%
ATCTGGATTCACACATAAGG+TGG - Chr4:20826084-20826103 MsG0480019474.01.T01:CDS 40.0%
ATTGTGATGACATACAAGGG+AGG + Chr4:20826677-20826696 None:intergenic 40.0%
CAAAGGGAAAGTGAACTTAC+GGG + Chr4:20825338-20825357 None:intergenic 40.0%
CTCAATCCTCAAAGCAGAAA+TGG - Chr4:20825079-20825098 MsG0480019474.01.T01:intron 40.0%
CTCGTGAAGTTAAGAGAGTA+TGG - Chr4:20825728-20825747 MsG0480019474.01.T01:intron 40.0%
CTTGGAAGAAAAGGATCAAG+CGG - Chr4:20824962-20824981 MsG0480019474.01.T01:intron 40.0%
GCTACCTAAAATGCTAAAGC+TGG + Chr4:20826006-20826025 None:intergenic 40.0%
GGAGTTAGAAGAACAAGATG+AGG - Chr4:20824582-20824601 MsG0480019474.01.T01:three_prime_UTR 40.0%
GGTTTGACACTCATCCAAAA+TGG - Chr4:20825488-20825507 MsG0480019474.01.T01:intron 40.0%
TCAATGAGGACACTACTGAT+TGG - Chr4:20825307-20825326 MsG0480019474.01.T01:intron 40.0%
TTGGAAGAAAAGGATCAAGC+GGG - Chr4:20824963-20824982 MsG0480019474.01.T01:intron 40.0%
! AATTTTCTGCGCTTCGCTAT+AGG + Chr4:20826472-20826491 None:intergenic 40.0%
! AGGTTGTTGTTGAGGAGATT+CGG - Chr4:20824845-20824864 MsG0480019474.01.T01:CDS 40.0%
! TCCATTTTATCTTCCCCAGT+TGG + Chr4:20825213-20825232 None:intergenic 40.0%
!! ATACTGATTTGCAATGCAGG+TGG - Chr4:20826389-20826408 MsG0480019474.01.T01:CDS 40.0%
!! GTGTTTGACTTGCATAACCT+TGG + Chr4:20825250-20825269 None:intergenic 40.0%
!! TTCTGCTTTGAGGATTGAGA+AGG + Chr4:20825078-20825097 None:intergenic 40.0%
ACAAGATGAGGAGTCTATGG+CGG - Chr4:20824594-20824613 MsG0480019474.01.T01:three_prime_UTR 45.0%
AGAAAAGGATCAAGCGGGAT+AGG - Chr4:20824968-20824987 MsG0480019474.01.T01:intron 45.0%
AGATCAGGAGGAAGAAGAAG+AGG - Chr4:20824654-20824673 MsG0480019474.01.T01:three_prime_UTR 45.0%
AGCATGCATGCTATGGATCA+TGG - Chr4:20826565-20826584 MsG0480019474.01.T01:CDS 45.0%
AGCGAAGCGCAGAAAATTGA+AGG - Chr4:20826474-20826493 MsG0480019474.01.T01:CDS 45.0%
AGCGCAGAAAATTGAAGGAC+TGG - Chr4:20826479-20826498 MsG0480019474.01.T01:CDS 45.0%
AGGAGAGAAGAAATGAGACG+AGG - Chr4:20824821-20824840 MsG0480019474.01.T01:CDS 45.0%
AGGCAGAGGAATATGAAGCT+TGG - Chr4:20824944-20824963 MsG0480019474.01.T01:intron 45.0%
AGTGTCCTCATTGACCAGAT+GGG + Chr4:20825301-20825320 None:intergenic 45.0%
ATGCCATGATGCAGCAATCT+TGG + Chr4:20826045-20826064 None:intergenic 45.0%
ATGCTATGGATCATGGAGCA+CGG - Chr4:20826572-20826591 MsG0480019474.01.T01:CDS 45.0%
ATGGTATCTCTCTCCGACTT+TGG + Chr4:20824742-20824761 None:intergenic 45.0%
GAAAAGGATCAAGCGGGATA+GGG - Chr4:20824969-20824988 MsG0480019474.01.T01:intron 45.0%
GCGCAGAAAATTGAAGGACT+GGG - Chr4:20826480-20826499 MsG0480019474.01.T01:CDS 45.0%
GGAATATGACAGAGGAGGAA+AGG - Chr4:20825040-20825059 MsG0480019474.01.T01:intron 45.0%
GGACAAGACAATATTGCCCA+AGG - Chr4:20825230-20825249 MsG0480019474.01.T01:intron 45.0%
TAGTGTCCTCATTGACCAGA+TGG + Chr4:20825302-20825321 None:intergenic 45.0%
TCACCAAGATTGCTGCATCA+TGG - Chr4:20826039-20826058 MsG0480019474.01.T01:CDS 45.0%
TGGCTTAAGCATGCATGCTA+TGG - Chr4:20826558-20826577 MsG0480019474.01.T01:CDS 45.0%
TGTTGTTGAGGAGATTCGGA+AGG - Chr4:20824849-20824868 MsG0480019474.01.T01:CDS 45.0%
! ACTGATTCTGATGAGTGCAC+TGG - Chr4:20824697-20824716 MsG0480019474.01.T01:three_prime_UTR 45.0%
! CACTGATGATGAACTCAACG+AGG - Chr4:20824924-20824943 MsG0480019474.01.T01:intron 45.0%
!! GGAGAGAAGAAGACGGATTA+AGG - Chr4:20824615-20824634 MsG0480019474.01.T01:three_prime_UTR 45.0%
!! GGAGGCACTGTTGAAAGAAA+AGG - Chr4:20824999-20825018 MsG0480019474.01.T01:intron 45.0%
!!! AAGGGGTTTTCTAGGATGCT+AGG - Chr4:20826309-20826328 MsG0480019474.01.T01:CDS 45.0%
!!! GAGAGAAGCAATGTTTTGGC+AGG + Chr4:20825435-20825454 None:intergenic 45.0%
AAGAACCAAGTGGTCCCATC+TGG - Chr4:20825284-20825303 MsG0480019474.01.T01:intron 50.0%
AGCAAGGAAGAGGAGAATGG+AGG - Chr4:20824801-20824820 MsG0480019474.01.T01:CDS 50.0%
ATTGACCAGATGGGACCACT+TGG + Chr4:20825292-20825311 None:intergenic 50.0%
CGATTTCTCTGCACCAACTG+GGG - Chr4:20825197-20825216 MsG0480019474.01.T01:intron 50.0%
CTATTGAAGAGGAGGCGAAG+AGG - Chr4:20824548-20824567 MsG0480019474.01.T01:three_prime_UTR 50.0%
CTCGGTCATCGGAATTAGAC+TGG + Chr4:20825143-20825162 None:intergenic 50.0%
CTCTTGAGGAAGCAAGGAAG+AGG - Chr4:20824791-20824810 MsG0480019474.01.T01:CDS 50.0%
GAAGTTGCGTCAGAGAGATC+AGG - Chr4:20824639-20824658 MsG0480019474.01.T01:three_prime_UTR 50.0%
GAGAAGAAATGAGACGAGGC+AGG - Chr4:20824825-20824844 MsG0480019474.01.T01:CDS 50.0%
GAGGGTGAGGAATATGACAG+AGG - Chr4:20825032-20825051 MsG0480019474.01.T01:intron 50.0%
GCGATTTCTCTGCACCAACT+GGG - Chr4:20825196-20825215 MsG0480019474.01.T01:intron 50.0%
GGAAGCAAGGAAGAGGAGAA+TGG - Chr4:20824798-20824817 MsG0480019474.01.T01:CDS 50.0%
GGTGAGGAATATGACAGAGG+AGG - Chr4:20825035-20825054 MsG0480019474.01.T01:intron 50.0%
GTCAAACACTTTGGTCGCAG+TGG - Chr4:20825261-20825280 MsG0480019474.01.T01:intron 50.0%
TGAGTGCACTGGAGTAGCTA+TGG - Chr4:20824708-20824727 MsG0480019474.01.T01:three_prime_UTR 50.0%
TGATGAACTCAACGAGGCAG+AGG - Chr4:20824930-20824949 MsG0480019474.01.T01:intron 50.0%
! CTATGGCGGAGAGAAGAAGA+CGG - Chr4:20824608-20824627 MsG0480019474.01.T01:three_prime_UTR 50.0%
AACAGTGGCAGCTCGGTCAT+CGG + Chr4:20825154-20825173 None:intergenic 55.0%
AAGGATCAAGCGGGATAGGG+AGG - Chr4:20824972-20824991 MsG0480019474.01.T01:intron 55.0%
AGCTGAAGAGGAGGCTCTTG+AGG - Chr4:20824777-20824796 MsG0480019474.01.T01:exon 55.0%
AGCTTCGAGACGCTCAGCAA+TGG + Chr4:20824761-20824780 None:intergenic 55.0%
AGGAAGAGCTTGAGAGGGTG+AGG - Chr4:20825019-20825038 MsG0480019474.01.T01:intron 55.0%
AGGAGGCTCTTGAGGAAGCA+AGG - Chr4:20824785-20824804 MsG0480019474.01.T01:exon 55.0%
CAGTTCCAACAGTGGCAGCT+CGG + Chr4:20825161-20825180 None:intergenic 55.0%
CGCGATTTCTCTGCACCAAC+TGG - Chr4:20825195-20825214 MsG0480019474.01.T01:intron 55.0%
GGAGCACGGTGTATGCAGAT+TGG - Chr4:20826586-20826605 MsG0480019474.01.T01:CDS 55.0%
GGAGGAAGAAGAAGAGGACG+AGG - Chr4:20824660-20824679 MsG0480019474.01.T01:three_prime_UTR 55.0%
GTCGCAGTGGAAGAACCAAG+TGG - Chr4:20825274-20825293 MsG0480019474.01.T01:intron 55.0%
GTGGTCCCATCTGGTCAATG+AGG - Chr4:20825293-20825312 MsG0480019474.01.T01:intron 55.0%
GTTGCGTCAGAGAGATCAGG+AGG - Chr4:20824642-20824661 MsG0480019474.01.T01:three_prime_UTR 55.0%
TGAGCGTCTCGAAGCTGAAG+AGG - Chr4:20824765-20824784 MsG0480019474.01.T01:three_prime_UTR 55.0%
! GACGAGGCAGGTTGTTGTTG+AGG - Chr4:20824837-20824856 MsG0480019474.01.T01:CDS 55.0%
AGGAGGCGAAGAGGCAGGAA+GGG - Chr4:20824557-20824576 MsG0480019474.01.T01:three_prime_UTR 60.0%
GATGACCGAGCTGCCACTGT+TGG - Chr4:20825153-20825172 MsG0480019474.01.T01:intron 60.0%
GCGTCTCGAAGCTGAAGAGG+AGG - Chr4:20824768-20824787 MsG0480019474.01.T01:exon 60.0%
TCAAGCGGGATAGGGAGGAG+CGG - Chr4:20824977-20824996 MsG0480019474.01.T01:intron 60.0%
TGAAGAGGAGGCGAAGAGGC+AGG - Chr4:20824552-20824571 MsG0480019474.01.T01:three_prime_UTR 60.0%
CAAGCGGGATAGGGAGGAGC+GGG - Chr4:20824978-20824997 MsG0480019474.01.T01:intron 65.0%
GAGGAGGCGAAGAGGCAGGA+AGG - Chr4:20824556-20824575 MsG0480019474.01.T01:three_prime_UTR 65.0%
GGCGAAGAGGCAGGAAGGGT+TGG - Chr4:20824561-20824580 MsG0480019474.01.T01:three_prime_UTR 65.0%
GCGGGATAGGGAGGAGCGGG+AGG - Chr4:20824981-20825000 MsG0480019474.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 20824514 20826785 20824514 ID=MsG0480019474.01;Name=MsG0480019474.01
Chr4 mRNA 20824514 20826785 20824514 ID=MsG0480019474.01.T01;Parent=MsG0480019474.01;Name=MsG0480019474.01.T01;_AED=0.49;_eAED=0.49;_QI=0|1|0.5|1|1|1|2|272|309
Chr4 exon 20824514 20824891 20824514 ID=MsG0480019474.01.T01:exon:10077;Parent=MsG0480019474.01.T01
Chr4 exon 20825962 20826785 20825962 ID=MsG0480019474.01.T01:exon:10076;Parent=MsG0480019474.01.T01
Chr4 CDS 20825962 20826785 20825962 ID=MsG0480019474.01.T01:cds;Parent=MsG0480019474.01.T01
Chr4 CDS 20824786 20824891 20824786 ID=MsG0480019474.01.T01:cds;Parent=MsG0480019474.01.T01
Chr4 three_prime_UTR 20824514 20824785 20824514 ID=MsG0480019474.01.T01:three_prime_utr;Parent=MsG0480019474.01.T01
Gene Sequence

>MsG0480019474.01.T01

ATGAATCGTTATTGGCAGGCTGAGATTGTTTCTACTATTGAAGAGGAGGCGAAGAGGCAGGAAGGGTTGGAGTTAGAAGAACAAGATGAGGAGTCTATGGCGGAGAGAAGAAGACGGATTAAGGAGAAGTTGCGTCAGAGAGATCAGGAGGAAGAAGAAGAGGACGAGGAGTCTGAGTATGATACTGATTCTGATGAGTGCACTGGAGTAGCTATGGTGTTTGTTCCAAAGTCGGAGAGAGATACCATTGCTGAGCGTCTCGAAGCTGAAGAGGAGGCTCTTGAGGAAGCAAGGAAGAGGAGAATGGAGGAGAGAAGAAATGAGACGAGGCAGGTTGTTGTTGAGGAGATTCGGAAGGATCAAGAAATTCAGAAAAATATTGAATTGGAGGTGAATATTGCTGATGTTGACACTGATGATGAACTCAACGAGGCAGAGGAATATGAAGCTTGGAAGAAAAGGATCAAGCGGGATAGGGAGGAGCGGGAGGCACTGTTGAAAGAAAAGGAAGAGCTTGAGAGGGTGAGGAATATGACAGAGGAGGAAAGGAAGAATCCAAAACCTTCTCAATCCTCAAAGCAGAAATGGAGGTTTATGCAGAAATACTATCACAAGGGTGCTTTCTTCCAGTCTAATTCCGATGACCGAGCTGCCACTGTTGGAACTGATAATATTTTCACGCGCGATTTCTCTGCACCAACTGGGGAAGATAAAATGGACAAGACAATATTGCCCAAGGTTATGCAAGTCAAACACTTTGGTCGCAGTGGAAGAACCAAGTGGTCCCATCTGGTCAATGAGGACACTACTGATTGGAACAACCCGTGGACTTACAATGATCGACTTCGAGCCAAGTATAATGAGAGAATGGCACAAATGAACGCGCCTATAGCGAAGCGCAGAAAATTGAAGGACTGGGAATCTCGATGA
Protein sequence

>MsG0480019474.01.T01

MNRYWQAEIVSTIEEEAKRQEGLELEEQDEESMAERRRRIKEKLRQRDQEEEEEDEESEYDTDSDECTGVAMVFVPKSERDTIAERLEAEEEALEEARKRRMEERRNETRQVVVEEIRKDQEIQKNIELEVNIADVDTDDELNEAEEYEAWKKRIKRDREEREALLKEKEELERVRNMTEEERKNPKPSQSSKQKWRFMQKYYHKGAFFQSNSDDRAATVGTDNIFTRDFSAPTGEDKMDKTILPKVMQVKHFGRSGRTKWSHLVNEDTTDWNNPWTYNDRLRAKYNERMAQMNAPIAKRRKLKDWESR*