AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021695.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021695.01.T01 MTR_4g075250 95.192 104 5 0 21 124 308 411 2.01e-68 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021695.01.T01 AT3G12400 65.657 99 33 1 23 120 299 397 4.32e-40 137
MsG0480021695.01.T01 AT3G12400 66.316 95 31 1 23 116 299 393 1.53e-38 134
MsG0480021695.01.T01 AT3G12400 66.316 95 31 1 23 116 299 393 3.07e-38 134
MsG0480021695.01.T01 AT5G13860 61.165 103 38 1 20 120 265 367 3.22e-37 129

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTTAGATAAGGGTGTTC+AGG 0.272232 4:+63035140 MsG0480021695.01.T01:CDS
GGAGAATCAAGGGAAGAAAA+TGG 0.279130 4:+63035014 MsG0480021695.01.T01:CDS
GCAGGCTCAGGTTGCGAATA+TGG 0.279429 4:+63035266 MsG0480021695.01.T01:CDS
TAGATAAGGGTGTTCAGGTT+GGG 0.287353 4:+63035145 MsG0480021695.01.T01:CDS
AGGGAAGAAAATGGGGAGTT+TGG 0.299911 4:+63035023 MsG0480021695.01.T01:CDS
TGATTGTACTTCTGCTGATT+TGG 0.313474 4:+63035098 MsG0480021695.01.T01:CDS
GAGAATCAAGGGAAGAAAAT+GGG 0.338918 4:+63035015 MsG0480021695.01.T01:CDS
TTTGATGAATACGGATGTTC+TGG 0.345033 4:+63034979 MsG0480021695.01.T01:CDS
AGTTAGAGCTGCTCAGTTGC+AGG 0.360251 4:+63035248 MsG0480021695.01.T01:CDS
ACGCTTTATGCTTTAGATAA+GGG 0.364897 4:+63035132 MsG0480021695.01.T01:CDS
AGCTGCTCAGTTGCAGGCTC+AGG 0.393395 4:+63035254 MsG0480021695.01.T01:CDS
AGATAAGGGTGTTCAGGTTG+GGG 0.397945 4:+63035146 MsG0480021695.01.T01:CDS
TACGCTTTATGCTTTAGATA+AGG 0.408278 4:+63035131 MsG0480021695.01.T01:CDS
GCTGCTAGGAATCATCATTA+TGG 0.423124 4:+63035288 MsG0480021695.01.T01:CDS
TGCAAGATGAGATGGAGGTT+TGG 0.424311 4:+63034937 None:intergenic
AGTACAATCAAGCATCTGTT+TGG 0.430905 4:-63035085 None:intergenic
TACCGTTTGATCAGTATTTG+AGG 0.441585 4:+63035172 MsG0480021695.01.T01:CDS
CTCCTCAAATACTGATCAAA+CGG 0.461994 4:-63035174 None:intergenic
TTAGATAAGGGTGTTCAGGT+TGG 0.491802 4:+63035144 MsG0480021695.01.T01:CDS
ATGTTCTGGAGGGTTGTTGA+GGG 0.504409 4:+63034993 MsG0480021695.01.T01:CDS
GGTTGTTGAGGGAGAATCAA+GGG 0.512007 4:+63035004 MsG0480021695.01.T01:CDS
GGAGATGCAAGATGAGATGG+AGG 0.528342 4:+63034932 None:intergenic
GAGTGTGAGGGCGTTGTCGA+GGG 0.528924 4:+63035194 MsG0480021695.01.T01:CDS
GAAGGAGATGCAAGATGAGA+TGG 0.537662 4:+63034929 None:intergenic
AGAATCAAGGGAAGAAAATG+GGG 0.538001 4:+63035016 MsG0480021695.01.T01:CDS
GGGTTGTTGAGGGAGAATCA+AGG 0.548744 4:+63035003 MsG0480021695.01.T01:CDS
TTTGGAGCAGCAGTTGCAGA+TGG 0.557427 4:+63034955 MsG0480021695.01.T01:CDS
GCAGTTCTTCCACCGTGCTA+CGG 0.559208 4:+63035218 MsG0480021695.01.T01:CDS
GGGGAGTTTGGAGAATGTTG+AGG 0.560828 4:+63035035 MsG0480021695.01.T01:CDS
ATCAGTATTTGAGGAGTGTG+AGG 0.563552 4:+63035181 MsG0480021695.01.T01:CDS
GGAGTGTGAGGGCGTTGTCG+AGG 0.566472 4:+63035193 MsG0480021695.01.T01:CDS
GATGTTCTGGAGGGTTGTTG+AGG 0.577268 4:+63034992 MsG0480021695.01.T01:CDS
GAGTTTGGAGAATGTTGAGG+AGG 0.585981 4:+63035038 MsG0480021695.01.T01:CDS
AGGTTGCGAATATGGCTGCT+AGG 0.587435 4:+63035274 MsG0480021695.01.T01:CDS
GATGAATACGGATGTTCTGG+AGG 0.588358 4:+63034982 MsG0480021695.01.T01:CDS
AATCATCATTATGGTAGCTG+AGG 0.620092 4:+63035297 MsG0480021695.01.T01:CDS
TAACTTTAGCAGCCGTAGCA+CGG 0.627369 4:-63035230 None:intergenic
TCAGTATTTGAGGAGTGTGA+GGG 0.644098 4:+63035182 MsG0480021695.01.T01:CDS
ATGAATACGGATGTTCTGGA+GGG 0.655370 4:+63034983 MsG0480021695.01.T01:CDS
CTTTAGCAGCCGTAGCACGG+TGG 0.725711 4:-63035227 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACGCTTTATGCTTTAGATAA+GGG + Chr4:63035132-63035151 MsG0480021695.01.T01:CDS 30.0%
! TACGCTTTATGCTTTAGATA+AGG + Chr4:63035131-63035150 MsG0480021695.01.T01:CDS 30.0%
AGAATCAAGGGAAGAAAATG+GGG + Chr4:63035016-63035035 MsG0480021695.01.T01:CDS 35.0%
AGTACAATCAAGCATCTGTT+TGG - Chr4:63035088-63035107 None:intergenic 35.0%
CTCCTCAAATACTGATCAAA+CGG - Chr4:63035177-63035196 None:intergenic 35.0%
GAGAATCAAGGGAAGAAAAT+GGG + Chr4:63035015-63035034 MsG0480021695.01.T01:CDS 35.0%
TACCGTTTGATCAGTATTTG+AGG + Chr4:63035172-63035191 MsG0480021695.01.T01:CDS 35.0%
TTTGATGAATACGGATGTTC+TGG + Chr4:63034979-63034998 MsG0480021695.01.T01:CDS 35.0%
! TGATTGTACTTCTGCTGATT+TGG + Chr4:63035098-63035117 MsG0480021695.01.T01:CDS 35.0%
!!! GCAGATGGTTTTGATGAATA+CGG + Chr4:63034970-63034989 MsG0480021695.01.T01:CDS 35.0%
GCTGCTAGGAATCATCATTA+TGG + Chr4:63035288-63035307 MsG0480021695.01.T01:CDS 40.0%
GGAGAATCAAGGGAAGAAAA+TGG + Chr4:63035014-63035033 MsG0480021695.01.T01:CDS 40.0%
! ATCAGTATTTGAGGAGTGTG+AGG + Chr4:63035181-63035200 MsG0480021695.01.T01:CDS 40.0%
! ATGAATACGGATGTTCTGGA+GGG + Chr4:63034983-63035002 MsG0480021695.01.T01:CDS 40.0%
! TCAGTATTTGAGGAGTGTGA+GGG + Chr4:63035182-63035201 MsG0480021695.01.T01:CDS 40.0%
!! TAGATAAGGGTGTTCAGGTT+GGG + Chr4:63035145-63035164 MsG0480021695.01.T01:CDS 40.0%
!! TGCTTTAGATAAGGGTGTTC+AGG + Chr4:63035140-63035159 MsG0480021695.01.T01:CDS 40.0%
!! TTAGATAAGGGTGTTCAGGT+TGG + Chr4:63035144-63035163 MsG0480021695.01.T01:CDS 40.0%
AGGGAAGAAAATGGGGAGTT+TGG + Chr4:63035023-63035042 MsG0480021695.01.T01:CDS 45.0%
GAGTTTGGAGAATGTTGAGG+AGG + Chr4:63035038-63035057 MsG0480021695.01.T01:CDS 45.0%
GATGAATACGGATGTTCTGG+AGG + Chr4:63034982-63035001 MsG0480021695.01.T01:CDS 45.0%
GGTTGTTGAGGGAGAATCAA+GGG + Chr4:63035004-63035023 MsG0480021695.01.T01:CDS 45.0%
TAACTTTAGCAGCCGTAGCA+CGG - Chr4:63035233-63035252 None:intergenic 45.0%
!! AGATAAGGGTGTTCAGGTTG+GGG + Chr4:63035146-63035165 MsG0480021695.01.T01:CDS 45.0%
!! ATGTTCTGGAGGGTTGTTGA+GGG + Chr4:63034993-63035012 MsG0480021695.01.T01:CDS 45.0%
AGGTTGCGAATATGGCTGCT+AGG + Chr4:63035274-63035293 MsG0480021695.01.T01:CDS 50.0%
AGTTAGAGCTGCTCAGTTGC+AGG + Chr4:63035248-63035267 MsG0480021695.01.T01:CDS 50.0%
GGGGAGTTTGGAGAATGTTG+AGG + Chr4:63035035-63035054 MsG0480021695.01.T01:CDS 50.0%
GGGTTGTTGAGGGAGAATCA+AGG + Chr4:63035003-63035022 MsG0480021695.01.T01:CDS 50.0%
! TTTGGAGCAGCAGTTGCAGA+TGG + Chr4:63034955-63034974 MsG0480021695.01.T01:CDS 50.0%
!! GATGTTCTGGAGGGTTGTTG+AGG + Chr4:63034992-63035011 MsG0480021695.01.T01:CDS 50.0%
!! TGAGGAGGCTTTTGAGTGTG+TGG + Chr4:63035053-63035072 MsG0480021695.01.T01:CDS 50.0%
GCAGTTCTTCCACCGTGCTA+CGG + Chr4:63035218-63035237 MsG0480021695.01.T01:CDS 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG + Chr4:63035266-63035285 MsG0480021695.01.T01:CDS 55.0%
AGCTGCTCAGTTGCAGGCTC+AGG + Chr4:63035254-63035273 MsG0480021695.01.T01:CDS 60.0%
CTTTAGCAGCCGTAGCACGG+TGG - Chr4:63035230-63035249 None:intergenic 60.0%
!! GAGTGTGAGGGCGTTGTCGA+GGG + Chr4:63035194-63035213 MsG0480021695.01.T01:CDS 60.0%
!! GGAGTGTGAGGGCGTTGTCG+AGG + Chr4:63035193-63035212 MsG0480021695.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63034943 63035317 63034943 ID=MsG0480021695.01;Name=MsG0480021695.01
Chr4 mRNA 63034943 63035317 63034943 ID=MsG0480021695.01.T01;Parent=MsG0480021695.01;Name=MsG0480021695.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|124
Chr4 exon 63034943 63035317 63034943 ID=MsG0480021695.01.T01:exon:9374;Parent=MsG0480021695.01.T01
Chr4 CDS 63034943 63035317 63034943 ID=MsG0480021695.01.T01:cds;Parent=MsG0480021695.01.T01
Gene Sequence

>MsG0480021695.01.T01

ATGAGATGGAGGTTTGGAGCAGCAGTTGCAGATGGTTTTGATGAATACGGATGTTCTGGAGGGTTGTTGAGGGAGAATCAAGGGAAGAAAATGGGGAGTTTGGAGAATGTTGAGGAGGCTTTTGAGTGTGTGGATGTGCTTTCCAAACAGATGCTTGATTGTACTTCTGCTGATTTGGCTATTGAAGATACGCTTTATGCTTTAGATAAGGGTGTTCAGGTTGGGGCTGTACCGTTTGATCAGTATTTGAGGAGTGTGAGGGCGTTGTCGAGGGAGCAGTTCTTCCACCGTGCTACGGCTGCTAAAGTTAGAGCTGCTCAGTTGCAGGCTCAGGTTGCGAATATGGCTGCTAGGAATCATCATTATGGTAGCTGA

Protein sequence

>MsG0480021695.01.T01

MRWRFGAAVADGFDEYGCSGGLLRENQGKKMGSLENVEEAFECVDVLSKQMLDCTSADLAIEDTLYALDKGVQVGAVPFDQYLRSVRALSREQFFHRATAAKVRAAQLQAQVANMAARNHHYGS*