AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019978.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019978.01.T01 MTR_0004s0230 87.069 348 3 4 17 325 1 345 0.0 587
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019978.01.T01 AT4G27620 58.462 325 78 7 39 325 20 325 7.78e-119 345
MsG0480019978.01.T01 AT4G27620 58.462 325 78 7 39 325 20 325 7.78e-119 345
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27610 57.478 341 99 7 23 325 2 334 1.02e-114 335
MsG0480019978.01.T01 AT4G27620 65.417 240 66 4 87 325 39 262 3.94e-102 301

Find 75 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGATGGGGATAATAATT+TGG 0.202722 4:-31088568 MsG0480019978.01.T01:CDS
GCAGCAGCAATGGGTCTTCT+AGG 0.267393 4:-31090125 MsG0480019978.01.T01:CDS
CATCTCTTCTCTTCAGTTGT+TGG 0.315375 4:+31088838 None:intergenic
GACAGGAACTGGAGGTTCTA+TGG 0.341619 4:-31089096 MsG0480019978.01.T01:intron
GCAAAGAAAATGGTGTTGTC+TGG 0.358201 4:-31088989 MsG0480019978.01.T01:CDS
CACAAACTCTACAAAATATT+AGG 0.364047 4:+31088369 None:intergenic
ACCTTTGAGATTCTCGTGCT+TGG 0.365115 4:+31089975 None:intergenic
CAAAGAATTGCAGCAGAGAT+TGG 0.365336 4:-31088403 MsG0480019978.01.T01:CDS
GCTGAGTTTAGCAAAGAAAA+TGG 0.365446 4:-31088999 MsG0480019978.01.T01:CDS
TCCCCATCAAGATGCCCATT+TGG 0.381342 4:+31088579 None:intergenic
GAAAGATGAAATGGCTTTGA+AGG 0.394804 4:+31088939 None:intergenic
ATTCAATGGGGCACTCCCTT+TGG 0.399748 4:-31089036 MsG0480019978.01.T01:CDS
GAGCAGTTTGAAAGATGAAA+TGG 0.401032 4:+31088930 None:intergenic
CCTTTGAGATTCTCGTGCTT+GGG 0.405977 4:+31089976 None:intergenic
AATCCAAATGGGCATCTTGA+TGG 0.414572 4:-31088582 MsG0480019978.01.T01:CDS
GAATTGAAGGAGGCGATAGA+AGG 0.415769 4:-31088483 MsG0480019978.01.T01:CDS
TTTGACCTTCAGAGAGAGTT+CGG 0.423724 4:+31088422 None:intergenic
GAAGGCAGTTCAAGACGATC+AGG 0.425759 4:+31088957 None:intergenic
AGATTCTCGTGCTTGGGAGG+AGG 0.431281 4:+31089982 None:intergenic
TCGCCATGTGTATCAGAATC+TGG 0.433773 4:-31088903 MsG0480019978.01.T01:CDS
TCTTGGTTACAAGTTGAAAC+AGG 0.441741 4:-31090022 MsG0480019978.01.T01:CDS
AAGGCAAGTGGGAGAATTGA+AGG 0.442051 4:-31088496 MsG0480019978.01.T01:CDS
CCCCATGACAAATCATCTAT+AGG 0.442585 4:-31088636 MsG0480019978.01.T01:CDS
TTCTCGTGCTTGGGAGGAGG+GGG 0.443049 4:+31089985 None:intergenic
AGATGTTGAGTGAATTCAAT+GGG 0.449435 4:-31089049 MsG0480019978.01.T01:CDS
ACTAGAAACGGTAATCCAAA+TGG 0.450297 4:-31088594 MsG0480019978.01.T01:CDS
CAATTGCTTTCCTTAGCCGT+TGG 0.455759 4:+31088682 None:intergenic
GATTCTCGTGCTTGGGAGGA+GGG 0.465385 4:+31089983 None:intergenic
TTCCTATAGATGATTTGTCA+TGG 0.468157 4:+31088634 None:intergenic
ATTCTCGTGCTTGGGAGGAG+GGG 0.472437 4:+31089984 None:intergenic
ATCCAAATGGGCATCTTGAT+GGG 0.473458 4:-31088581 MsG0480019978.01.T01:CDS
AGTACATTGTCTATTCGTCT+TGG 0.477908 4:-31090039 MsG0480019978.01.T01:CDS
ACTAGAGATGCAAGATCAAA+TGG 0.484563 4:-31088808 MsG0480019978.01.T01:CDS
TCCTATAGATGATTTGTCAT+GGG 0.492632 4:+31088635 None:intergenic
AGCGAATTGAGATGCTGAAA+AGG 0.497757 4:-31088515 MsG0480019978.01.T01:CDS
CACAAAGCAGCAGCAGCAAT+GGG 0.501046 4:-31090134 MsG0480019978.01.T01:CDS
CAAAGAAAATGGTGTTGTCT+GGG 0.509421 4:-31088988 MsG0480019978.01.T01:CDS
TAGCCGTTGGATCTCTCTCT+CGG 0.521082 4:+31088695 None:intergenic
TACACGGTGGCGCAAGTGGA+TGG 0.524749 4:+31090337 None:intergenic
GAAGGAGGCGATAGAAGGAA+AGG 0.528178 4:-31088478 MsG0480019978.01.T01:CDS
AGAGAGAGATCCAACGGCTA+AGG 0.532052 4:-31088692 MsG0480019978.01.T01:CDS
GCAATTGCAGATCACTGTGT+TGG 0.533511 4:-31088666 MsG0480019978.01.T01:CDS
CAGCAGCAATGGGTCTTCTA+GGG 0.540637 4:-31090124 MsG0480019978.01.T01:CDS
TTGAGATTCTCGTGCTTGGG+AGG 0.541092 4:+31089979 None:intergenic
AATGAGAAGAAAGTCCGAGC+TGG 0.542815 4:+31088760 None:intergenic
CAGGAGGAACAGTCACCAAA+GGG 0.547059 4:+31089021 None:intergenic
ACATGGATTATTATTGCTGC+TGG 0.548295 4:-31090072 MsG0480019978.01.T01:CDS
GCACAAAGCAGCAGCAGCAA+TGG 0.548860 4:-31090135 MsG0480019978.01.T01:CDS
CTAGAAACGGTAATCCAAAT+GGG 0.553025 4:-31088593 MsG0480019978.01.T01:CDS
ATGAGAAGAAAGTCCGAGCT+GGG 0.556138 4:+31088761 None:intergenic
GGAATACACGGTGGCGCAAG+TGG 0.556451 4:+31090333 None:intergenic
CCTATAGATGATTTGTCATG+GGG 0.564452 4:+31088636 None:intergenic
AAGGCAGTTCAAGACGATCA+GGG 0.568099 4:+31088958 None:intergenic
AAAGTCCGAACTCTCTCTGA+AGG 0.571523 4:-31088427 MsG0480019978.01.T01:CDS
GCAGGAGGAACAGTCACCAA+AGG 0.580222 4:+31089020 None:intergenic
AATTCACTCAATGCCCAGCT+CGG 0.581833 4:-31088774 MsG0480019978.01.T01:CDS
ATACTTGCTCCAGAGCTACA+AGG 0.584521 4:-31088454 MsG0480019978.01.T01:CDS
TGGTCTGTTGACACTAGAAA+CGG 0.584679 4:-31088606 MsG0480019978.01.T01:CDS
TCCAAATGGGCATCTTGATG+GGG 0.605570 4:-31088580 MsG0480019978.01.T01:CDS
CAGATGTTGAGTGAATTCAA+TGG 0.616150 4:-31089050 MsG0480019978.01.T01:CDS
CCCAAGCACGAGAATCTCAA+AGG 0.616263 4:-31089976 MsG0480019978.01.T01:intron
AATCATCTATAGGAACAGCT+TGG 0.621119 4:-31088626 MsG0480019978.01.T01:CDS
TGAGATGCTGAAAAGGCAAG+TGG 0.628975 4:-31088508 MsG0480019978.01.T01:CDS
GAGGAGAGGAAGGACCAACA+TGG 0.631152 4:-31090089 MsG0480019978.01.T01:CDS
GCAGCAATAATAATCCATGT+TGG 0.633908 4:+31090075 None:intergenic
AGGCAGTTCAAGACGATCAG+GGG 0.644725 4:+31088959 None:intergenic
TCTTTGCTAAACTCAGCAGG+AGG 0.648645 4:+31089005 None:intergenic
ACTCCGAGAGAGAGATCCAA+CGG 0.659001 4:-31088698 MsG0480019978.01.T01:CDS
ACGGTGGCGCAAGTGGATGG+AGG 0.662456 4:+31090340 None:intergenic
GAGATGCTGAAAAGGCAAGT+GGG 0.663385 4:-31088507 MsG0480019978.01.T01:CDS
GAACCAGATTCTGATACACA+TGG 0.699682 4:+31088900 None:intergenic
GCAAGTGGGAGAATTGAAGG+AGG 0.729148 4:-31088493 MsG0480019978.01.T01:CDS
TGATGATGGACAGGAACTGG+AGG 0.738580 4:-31089104 MsG0480019978.01.T01:intron
CTATAGATGATTTGTCATGG+GGG 0.748801 4:+31088637 None:intergenic
GATGTTGAGTGAATTCAATG+GGG 0.772356 4:-31089048 MsG0480019978.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTGAATTATTATATTTAT+TGG - Chr4:31089464-31089483 MsG0480019978.01.T01:intron 10.0%
!!! TAATTTTCTAAAATTATTAG+CGG + Chr4:31089381-31089400 None:intergenic 10.0%
!! ATTATTATTATCTTGCATTT+GGG + Chr4:31088912-31088931 None:intergenic 15.0%
!! TATTATTATTATCTTGCATT+TGG + Chr4:31088913-31088932 None:intergenic 15.0%
!!! ATTATTTTTATTATGAAGCA+CGG - Chr4:31089026-31089045 MsG0480019978.01.T01:CDS 15.0%
!! AATAATTCACAAACTATTAG+CGG + Chr4:31089456-31089475 None:intergenic 20.0%
!! AATTAAATATTCCTAACTTC+AGG - Chr4:31088827-31088846 MsG0480019978.01.T01:CDS 20.0%
!!! TTGTTTTAAATGGATTTTGT+AGG - Chr4:31088529-31088548 MsG0480019978.01.T01:CDS 20.0%
!!! TTTTTTAAAATTAGCTGTGT+CGG + Chr4:31089099-31089118 None:intergenic 20.0%
! TGTGACATATACTTTCATTA+CGG - Chr4:31089564-31089583 MsG0480019978.01.T01:intron 25.0%
! TTAGATTATGTATGTGATGA+TGG - Chr4:31089602-31089621 MsG0480019978.01.T01:intron 25.0%
!! CCATTTAAAACAAAAACAGT+TGG + Chr4:31088522-31088541 None:intergenic 25.0%
!! TTCTGATATTTTCAGTAAGA+GGG - Chr4:31089840-31089859 MsG0480019978.01.T01:intron 25.0%
!!! ATTGTTGATAGTAGTTTTTG+TGG - Chr4:31089495-31089514 MsG0480019978.01.T01:intron 25.0%
!!! CCAACTGTTTTTGTTTTAAA+TGG - Chr4:31088519-31088538 MsG0480019978.01.T01:CDS 25.0%
ACTATATGTCACACAAAATG+CGG + Chr4:31089522-31089541 None:intergenic 30.0%
AGAAACTAAACAACAAGGAT+TGG + Chr4:31088999-31089018 None:intergenic 30.0%
AGATAAAGAAGACAGTTCAA+TGG + Chr4:31088772-31088791 None:intergenic 30.0%
AGATGTTGAGTGAATTCAAT+GGG - Chr4:31089671-31089690 MsG0480019978.01.T01:intron 30.0%
AGTATATGTCACACAAAATG+CGG + Chr4:31089557-31089576 None:intergenic 30.0%
AGTTAGAAGAAAGAGAGAAA+TGG + Chr4:31088443-31088462 None:intergenic 30.0%
ATATTCCTAACTTCAGGAAA+TGG - Chr4:31088833-31088852 MsG0480019978.01.T01:CDS 30.0%
CAAATCGAATAATTGCAATG+TGG - Chr4:31089246-31089265 MsG0480019978.01.T01:intron 30.0%
CACATTGCAATTATTCGATT+TGG + Chr4:31089248-31089267 None:intergenic 30.0%
CATAATCTAAGCCAGAAAAA+TGG + Chr4:31089592-31089611 None:intergenic 30.0%
GAGAAAGAAACTAAACAACA+AGG + Chr4:31089004-31089023 None:intergenic 30.0%
TATCCATTAAAGCAATCTGA+AGG + Chr4:31088887-31088906 None:intergenic 30.0%
TCTTGATGGGGATAATAATT+TGG - Chr4:31090152-31090171 MsG0480019978.01.T01:CDS 30.0%
TTCAGTGTAATTACAAGTGT+CGG - Chr4:31089406-31089425 MsG0480019978.01.T01:intron 30.0%
! ATTTTCCATTTCCTGAAGTT+AGG + Chr4:31088841-31088860 None:intergenic 30.0%
! GTGACATATAGTAGTTTTTG+TGG - Chr4:31089530-31089549 MsG0480019978.01.T01:intron 30.0%
! GTTCTGATATTTTCAGTAAG+AGG - Chr4:31089839-31089858 MsG0480019978.01.T01:intron 30.0%
! TATAGTTTGCATGTCTTTGT+TGG + Chr4:31089312-31089331 None:intergenic 30.0%
! TCCTATAGATGATTTGTCAT+GGG + Chr4:31090088-31090107 None:intergenic 30.0%
! TCTAAAATTATTAGCGGTGT+CGG + Chr4:31089375-31089394 None:intergenic 30.0%
! TTCCTATAGATGATTTGTCA+TGG + Chr4:31090089-31090108 None:intergenic 30.0%
!! TCTTCTAGGGTTCATAAAAA+TGG - Chr4:31088609-31088628 MsG0480019978.01.T01:CDS 30.0%
AATCATCTATAGGAACAGCT+TGG - Chr4:31090094-31090113 MsG0480019978.01.T01:CDS 35.0%
ACTAGAAACGGTAATCCAAA+TGG - Chr4:31090126-31090145 MsG0480019978.01.T01:CDS 35.0%
ACTAGAGATGCAAGATCAAA+TGG - Chr4:31089912-31089931 MsG0480019978.01.T01:intron 35.0%
AGTACATTGTCTATTCGTCT+TGG - Chr4:31088681-31088700 MsG0480019978.01.T01:CDS 35.0%
ATCGAATAATTGCAATGTGG+TGG - Chr4:31089249-31089268 MsG0480019978.01.T01:intron 35.0%
ATGGAAAATCCTCCAACATA+AGG - Chr4:31088852-31088871 MsG0480019978.01.T01:CDS 35.0%
CAGATGTTGAGTGAATTCAA+TGG - Chr4:31089670-31089689 MsG0480019978.01.T01:intron 35.0%
CTAGAAACGGTAATCCAAAT+GGG - Chr4:31090127-31090146 MsG0480019978.01.T01:CDS 35.0%
CTATATGCATCTATGAAGCA+CGG - Chr4:31089327-31089346 MsG0480019978.01.T01:intron 35.0%
GAGCAGTTTGAAAGATGAAA+TGG + Chr4:31089793-31089812 None:intergenic 35.0%
GATGTTGAGTGAATTCAATG+GGG - Chr4:31089672-31089691 MsG0480019978.01.T01:intron 35.0%
GCAGCAATAATAATCCATGT+TGG + Chr4:31088648-31088667 None:intergenic 35.0%
GCTGAGTTTAGCAAAGAAAA+TGG - Chr4:31089721-31089740 MsG0480019978.01.T01:intron 35.0%
GCTTCATGAGTCATAAAGTA+GGG - Chr4:31089207-31089226 MsG0480019978.01.T01:intron 35.0%
TCACCTTCAGATTGCTTTAA+TGG - Chr4:31088881-31088900 MsG0480019978.01.T01:CDS 35.0%
TGAAAAAACAAAGGATGAGG+AGG - Chr4:31090182-31090201 MsG0480019978.01.T01:intron 35.0%
TGCTTCATGAGTCATAAAGT+AGG - Chr4:31089206-31089225 MsG0480019978.01.T01:intron 35.0%
TTAAAGCAATCTGAAGGTGA+AGG + Chr4:31088881-31088900 None:intergenic 35.0%
! ACATGGATTATTATTGCTGC+TGG - Chr4:31088648-31088667 MsG0480019978.01.T01:CDS 35.0%
! CCTATAGATGATTTGTCATG+GGG + Chr4:31090087-31090106 None:intergenic 35.0%
! CTATAGATGATTTGTCATGG+GGG + Chr4:31090086-31090105 None:intergenic 35.0%
! TCTAGGGTTCATAAAAATGG+AGG - Chr4:31088612-31088631 MsG0480019978.01.T01:CDS 35.0%
! TCTTGGTTACAAGTTGAAAC+AGG - Chr4:31088698-31088717 MsG0480019978.01.T01:CDS 35.0%
! TTTTCTTTGCTAAACTCAGC+AGG + Chr4:31089721-31089740 None:intergenic 35.0%
!! CAAAGAAAATGGTGTTGTCT+GGG - Chr4:31089732-31089751 MsG0480019978.01.T01:intron 35.0%
!! GAAAGATGAAATGGCTTTGA+AGG + Chr4:31089784-31089803 None:intergenic 35.0%
!! TTATGTATGTGATGATGGAC+AGG - Chr4:31089607-31089626 MsG0480019978.01.T01:intron 35.0%
!!! CCTCATCCTTTGTTTTTTCA+GGG + Chr4:31090182-31090201 None:intergenic 35.0%
!!! TCCTCATCCTTTGTTTTTTC+AGG + Chr4:31090183-31090202 None:intergenic 35.0%
AATCCAAATGGGCATCTTGA+TGG - Chr4:31090138-31090157 MsG0480019978.01.T01:CDS 40.0%
ACCTGAATTGCAAGTGAAGA+AGG + Chr4:31088945-31088964 None:intergenic 40.0%
AGCGAATTGAGATGCTGAAA+AGG - Chr4:31090205-31090224 MsG0480019978.01.T01:intron 40.0%
AGGTGAAGGTTTCCTTATGT+TGG + Chr4:31088867-31088886 None:intergenic 40.0%
ATCCAAATGGGCATCTTGAT+GGG - Chr4:31090139-31090158 MsG0480019978.01.T01:CDS 40.0%
CAAAGAATTGCAGCAGAGAT+TGG - Chr4:31090317-31090336 MsG0480019978.01.T01:intron 40.0%
CATAAAAATGGAGGAGAGGA+AGG - Chr4:31088621-31088640 MsG0480019978.01.T01:CDS 40.0%
CATCTCTTCTCTTCAGTTGT+TGG + Chr4:31089885-31089904 None:intergenic 40.0%
CCCCATGACAAATCATCTAT+AGG - Chr4:31090084-31090103 MsG0480019978.01.T01:CDS 40.0%
CCCTGAAAAAACAAAGGATG+AGG - Chr4:31090179-31090198 MsG0480019978.01.T01:intron 40.0%
GAACCAGATTCTGATACACA+TGG + Chr4:31089823-31089842 None:intergenic 40.0%
GGACATCCCTGAAAAAACAA+AGG - Chr4:31090173-31090192 MsG0480019978.01.T01:intron 40.0%
GGTTCATAAAAATGGAGGAG+AGG - Chr4:31088617-31088636 MsG0480019978.01.T01:CDS 40.0%
TCATGAGTCATAAAGTAGGG+TGG - Chr4:31089210-31089229 MsG0480019978.01.T01:intron 40.0%
TGAAGGTTTCCTTATGTTGG+AGG + Chr4:31088864-31088883 None:intergenic 40.0%
TGGTCTGTTGACACTAGAAA+CGG - Chr4:31090114-31090133 MsG0480019978.01.T01:CDS 40.0%
TTTGACCTTCAGAGAGAGTT+CGG + Chr4:31090301-31090320 None:intergenic 40.0%
!! GCAAAGAAAATGGTGTTGTC+TGG - Chr4:31089731-31089750 MsG0480019978.01.T01:intron 40.0%
!!! TCATTACGGTGCCATTTTTC+TGG - Chr4:31089578-31089597 MsG0480019978.01.T01:intron 40.0%
AAAGTCCGAACTCTCTCTGA+AGG - Chr4:31090293-31090312 MsG0480019978.01.T01:intron 45.0%
AAGGCAAGTGGGAGAATTGA+AGG - Chr4:31090224-31090243 MsG0480019978.01.T01:intron 45.0%
AAGGCAGTTCAAGACGATCA+GGG + Chr4:31089765-31089784 None:intergenic 45.0%
AATGAGAAGAAAGTCCGAGC+TGG + Chr4:31089963-31089982 None:intergenic 45.0%
AATTCACTCAATGCCCAGCT+CGG - Chr4:31089946-31089965 MsG0480019978.01.T01:intron 45.0%
AGCGATGGTCTAGGAATACA+CGG + Chr4:31088402-31088421 None:intergenic 45.0%
ATACTTGCTCCAGAGCTACA+AGG - Chr4:31090266-31090285 MsG0480019978.01.T01:intron 45.0%
ATGAGAAGAAAGTCCGAGCT+GGG + Chr4:31089962-31089981 None:intergenic 45.0%
CAATTGCTTTCCTTAGCCGT+TGG + Chr4:31090041-31090060 None:intergenic 45.0%
GAATTGAAGGAGGCGATAGA+AGG - Chr4:31090237-31090256 MsG0480019978.01.T01:intron 45.0%
GAGATGCTGAAAAGGCAAGT+GGG - Chr4:31090213-31090232 MsG0480019978.01.T01:intron 45.0%
GCAATTGCAGATCACTGTGT+TGG - Chr4:31090054-31090073 MsG0480019978.01.T01:CDS 45.0%
GCCTTCTTCACTTGCAATTC+AGG - Chr4:31088941-31088960 MsG0480019978.01.T01:CDS 45.0%
TCCAAATGGGCATCTTGATG+GGG - Chr4:31090140-31090159 MsG0480019978.01.T01:CDS 45.0%
TCGCCATGTGTATCAGAATC+TGG - Chr4:31089817-31089836 MsG0480019978.01.T01:intron 45.0%
TCTTTGCTAAACTCAGCAGG+AGG + Chr4:31089718-31089737 None:intergenic 45.0%
TGAGATGCTGAAAAGGCAAG+TGG - Chr4:31090212-31090231 MsG0480019978.01.T01:intron 45.0%
TGAGTCATAAAGTAGGGTGG+TGG - Chr4:31089213-31089232 MsG0480019978.01.T01:intron 45.0%
! ACTTTTGCTCCTTGTAGCTC+TGG + Chr4:31090278-31090297 None:intergenic 45.0%
! TACAAGTGTCGTTGTCGTGT+CGG - Chr4:31089140-31089159 MsG0480019978.01.T01:intron 45.0%
!! ACCTTTGAGATTCTCGTGCT+TGG + Chr4:31088748-31088767 None:intergenic 45.0%
!! ATGTGATGATGGACAGGAAC+TGG - Chr4:31089613-31089632 MsG0480019978.01.T01:intron 45.0%
!! CCTTTGAGATTCTCGTGCTT+GGG + Chr4:31088747-31088766 None:intergenic 45.0%
!! TACAAGTGTCGGTGTTGTGT+CGG - Chr4:31089417-31089436 MsG0480019978.01.T01:intron 45.0%
!! AAAAATAAATAAATCAAAAA+AGG + Chr4:31088488-31088507 None:intergenic 5.0%
!! AAAATAAATAAATCAAAAAA+GGG + Chr4:31088487-31088506 None:intergenic 5.0%
ACTCCGAGAGAGAGATCCAA+CGG - Chr4:31090022-31090041 MsG0480019978.01.T01:CDS 50.0%
AGAGAGAGATCCAACGGCTA+AGG - Chr4:31090028-31090047 MsG0480019978.01.T01:CDS 50.0%
AGGCAGTTCAAGACGATCAG+GGG + Chr4:31089764-31089783 None:intergenic 50.0%
CACAAAGCAGCAGCAGCAAT+GGG - Chr4:31088586-31088605 MsG0480019978.01.T01:CDS 50.0%
CAGCAGCAATGGGTCTTCTA+GGG - Chr4:31088596-31088615 MsG0480019978.01.T01:CDS 50.0%
CAGGAGGAACAGTCACCAAA+GGG + Chr4:31089702-31089721 None:intergenic 50.0%
CATGAAGCAGAGACACTCCT+CGG + Chr4:31089195-31089214 None:intergenic 50.0%
CCCAAGCACGAGAATCTCAA+AGG - Chr4:31088744-31088763 MsG0480019978.01.T01:CDS 50.0%
CTAGACCATCGCTGTTCTAC+AGG - Chr4:31088409-31088428 MsG0480019978.01.T01:CDS 50.0%
CTGTAGAACAGCGATGGTCT+AGG + Chr4:31088411-31088430 None:intergenic 50.0%
GAAGGAGGCGATAGAAGGAA+AGG - Chr4:31090242-31090261 MsG0480019978.01.T01:intron 50.0%
GAAGGCAGTTCAAGACGATC+AGG + Chr4:31089766-31089785 None:intergenic 50.0%
GACAGGAACTGGAGGTTCTA+TGG - Chr4:31089624-31089643 MsG0480019978.01.T01:intron 50.0%
GATGGTCTAGGAATACACGG+TGG + Chr4:31088399-31088418 None:intergenic 50.0%
GCAAGTGGGAGAATTGAAGG+AGG - Chr4:31090227-31090246 MsG0480019978.01.T01:intron 50.0%
TAGCCGTTGGATCTCTCTCT+CGG + Chr4:31090028-31090047 None:intergenic 50.0%
TCCCCATCAAGATGCCCATT+TGG + Chr4:31090144-31090163 None:intergenic 50.0%
TTGAGATTCTCGTGCTTGGG+AGG + Chr4:31088744-31088763 None:intergenic 50.0%
! ATTCAATGGGGCACTCCCTT+TGG - Chr4:31089684-31089703 MsG0480019978.01.T01:intron 50.0%
!! TGATGATGGACAGGAACTGG+AGG - Chr4:31089616-31089635 MsG0480019978.01.T01:intron 50.0%
AGATTCTCGTGCTTGGGAGG+AGG + Chr4:31088741-31088760 None:intergenic 55.0%
ATTCTCGTGCTTGGGAGGAG+GGG + Chr4:31088739-31088758 None:intergenic 55.0%
CACAGACACCAGACACGACA+CGG - Chr4:31089056-31089075 MsG0480019978.01.T01:CDS 55.0%
GAGGAGAGGAAGGACCAACA+TGG - Chr4:31088631-31088650 MsG0480019978.01.T01:CDS 55.0%
GATTAGGCGTGTCCTGGTGT+CGG + Chr4:31089173-31089192 None:intergenic 55.0%
GATTCTCGTGCTTGGGAGGA+GGG + Chr4:31088740-31088759 None:intergenic 55.0%
GCACAAAGCAGCAGCAGCAA+TGG - Chr4:31088585-31088604 MsG0480019978.01.T01:CDS 55.0%
GCAGAGACACTCCTCGGATT+AGG + Chr4:31089189-31089208 None:intergenic 55.0%
GCAGCAGCAATGGGTCTTCT+AGG - Chr4:31088595-31088614 MsG0480019978.01.T01:CDS 55.0%
GCAGGAGGAACAGTCACCAA+AGG + Chr4:31089703-31089722 None:intergenic 55.0%
!! AAGTGTCGGTGTTGTGTCGG+TGG - Chr4:31089420-31089439 MsG0480019978.01.T01:intron 55.0%
!! AGTGTCGGTGTTGTGTCGGT+GGG - Chr4:31089421-31089440 MsG0480019978.01.T01:intron 55.0%
ACCAGGACACGCCTAATCCG+AGG - Chr4:31089175-31089194 MsG0480019978.01.T01:intron 60.0%
GCGTGCCTGTAGAACAGCGA+TGG + Chr4:31088417-31088436 None:intergenic 60.0%
GGAATACACGGTGGCGCAAG+TGG + Chr4:31088390-31088409 None:intergenic 60.0%
GTCAGTGTCCGTGTCGTGTC+TGG + Chr4:31089067-31089086 None:intergenic 60.0%
TACACGGTGGCGCAAGTGGA+TGG + Chr4:31088386-31088405 None:intergenic 60.0%
TTCTCGTGCTTGGGAGGAGG+GGG + Chr4:31088738-31088757 None:intergenic 60.0%
! GGTGTTGTGTCGGTGGGTGT+CGG - Chr4:31089427-31089446 MsG0480019978.01.T01:intron 60.0%
! TCCTCGGATTAGGCGTGTCC+TGG + Chr4:31089179-31089198 None:intergenic 60.0%
ACGGTGGCGCAAGTGGATGG+AGG + Chr4:31088383-31088402 None:intergenic 65.0%
GTCGGTACGTGTCCGACACC+AGG - Chr4:31089158-31089177 MsG0480019978.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 31088377 31090365 31088377 ID=MsG0480019978.01;Name=MsG0480019978.01
Chr4 mRNA 31088377 31090365 31088377 ID=MsG0480019978.01.T01;Parent=MsG0480019978.01;Name=MsG0480019978.01.T01;_AED=0.38;_eAED=0.41;_QI=0|0|0|1|1|1|3|0|325
Chr4 exon 31090340 31090365 31090340 ID=MsG0480019978.01.T01:exon:12775;Parent=MsG0480019978.01.T01
Chr4 exon 31089977 31090191 31089977 ID=MsG0480019978.01.T01:exon:12774;Parent=MsG0480019978.01.T01
Chr4 exon 31088377 31089113 31088377 ID=MsG0480019978.01.T01:exon:12773;Parent=MsG0480019978.01.T01
Chr4 CDS 31090340 31090365 31090340 ID=MsG0480019978.01.T01:cds;Parent=MsG0480019978.01.T01
Chr4 CDS 31089977 31090191 31089977 ID=MsG0480019978.01.T01:cds;Parent=MsG0480019978.01.T01
Chr4 CDS 31088377 31089113 31088377 ID=MsG0480019978.01.T01:cds;Parent=MsG0480019978.01.T01
Gene Sequence

>MsG0480019978.01.T01

ATGCCTCCATCCACTTGCGCCACCGTGTTGTTTTTGAGTGTTAATAAAATGAAAATGAAAGCACAAAGCAGCAGCAGCAATGGGTCTTCTAGGGTTCATAAAAATGGAGGAGAGGAAGGACCAACATGGATTATTATTGCTGCTGGTGCTTTGTTGAGTACATTGTCTATTCGTCTTGGTTACAAGTTGAAACAGGCTATTGACTCAAAACCCCCTCCTCCCAAGCACGAGAATCTCAAAGGAACTGGAGGTTCTATGGAACTAAAGCGCGCACAAAGTGAACAGATGTTGAGTGAATTCAATGGGGCACTCCCTTTGGTGACTGTTCCTCCTGCTGAGTTTAGCAAAGAAAATGGTGTTGTCTGGGCTTGCTCCCCTGATCGTCTTGAACTGCCTTCAAAGCCATTTCATCTTTCAAACTGCTCAGATTCGCCATGTGTATCAGAATCTGGTTCTGATATTTTCAGTAAGAGGGAAGTCATACAGAAACTGCGCCAACAACTGAAGAGAAGAGATGATATGATACTAGAGATGCAAGATCAAATGGCTGAATTGCAAAATTCACTCAATGCCCAGCTCGGACTTTCTTCTCATTTGCAATTGCAGCTTGAAGCTGCAAACAGAGACTTATTTGACTCCGAGAGAGAGATCCAACGGCTAAGGAAAGCAATTGCAGATCACTGTGTTGGATATGTCCCCCATGACAAATCATCTATAGGAACAGCTTGGTCTGTTGACACTAGAAACGGTAATCCAAATGGGCATCTTGATGGGGATAATAATTTGGACATCCCTGAAAAAACAAAGGATGAGGAGGAGCGAATTGAGATGCTGAAAAGGCAAGTGGGAGAATTGAAGGAGGCGATAGAAGGAAAGGAATACTTGCTCCAGAGCTACAAGGAGCAAAAGTCCGAACTCTCTCTGAAGGTCAAAGAATTGCAGCAGAGATTGGATTCTCAGCTGCCTAATATTTTGTAG
Protein sequence

>MsG0480019978.01.T01

MPPSTCATVLFLSVNKMKMKAQSSSSNGSSRVHKNGGEEGPTWIIIAAGALLSTLSIRLGYKLKQAIDSKPPPPKHENLKGTGGSMELKRAQSEQMLSEFNGALPLVTVPPAEFSKENGVVWACSPDRLELPSKPFHLSNCSDSPCVSESGSDIFSKREVIQKLRQQLKRRDDMILEMQDQMAELQNSLNAQLGLSSHLQLQLEAANRDLFDSEREIQRLRKAIADHCVGYVPHDKSSIGTAWSVDTRNGNPNGHLDGDNNLDIPEKTKDEEERIEMLKRQVGELKEAIEGKEYLLQSYKEQKSELSLKVKELQQRLDSQLPNIL*