AlfalfaGEDB | MsG0480023039.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023039.01

KEGG: -
GO: -
PFAM: PF14368

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023039.01.T01	XP_003629697.1	97.279	147	4	0	1	147	1	147	4.02E-97	288

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023039.01.T01	O64865	44.444	144	78	1	6	147	3	146	1.29E-38	133

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023039.01.T01	G7LHX9	97.279	147	4	0	1	147	1	147	1.92e-97	288

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000670.01	MsG0480023039.01	0.820958	5.208232e-53	4.181482e-50
MsG0180001175.01	MsG0480023039.01	0.802168	6.375199e-49	3.101520e-46
MsG0180005135.01	MsG0480023039.01	0.820900	5.371202e-53	4.305028e-50
MsG0180005485.01	MsG0480023039.01	0.812658	3.795616e-51	2.427311e-48
MsG0180005964.01	MsG0480023039.01	0.818528	1.870045e-52	1.403329e-49
MsG0480018644.01	MsG0480023039.01	0.837355	5.457970e-57	7.071173e-54
MsG0480023039.01	MsG0580025366.01	0.808131	3.601320e-50	2.042581e-47
MsG0480023039.01	MsG0580025502.01	0.817335	3.478834e-52	2.526307e-49
MsG0480023039.01	MsG0780039675.01	0.824283	8.776035e-54	7.738571e-51
MsG0480023039.01	MsG0780041174.01	0.823714	1.193835e-53	1.036141e-50
MsG0480023039.01	MsG0880045101.01	0.844137	9.104612e-59	1.456173e-55
MsG0380016771.01	MsG0480023039.01	0.810275	1.249864e-50	7.501505e-48
MsG0280008388.01	MsG0480023039.01	0.832204	1.080059e-55	1.198817e-52
MsG0280008394.01	MsG0480023039.01	0.828566	8.372875e-55	8.353504e-52
MsG0280008451.01	MsG0480023039.01	0.802733	4.877896e-49	2.407510e-46
MsG0280008591.01	MsG0480023039.01	0.806711	7.203355e-50	3.937150e-47
MsG0280010353.01	MsG0480023039.01	0.820359	7.152400e-53	5.646860e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023039.01.T01	MTR_8g085560	97.279	147	4	0	1	147	1	147	4.86e-101	288
MsG0480023039.01.T01	MTR_3g083760	53.600	125	58	0	23	147	18	142	8.29e-48	152
MsG0480023039.01.T01	MTR_3g083760	47.482	139	56	1	23	144	18	156	1.91e-42	140
MsG0480023039.01.T01	MTR_8g446830	43.939	132	73	1	15	145	13	144	3.26e-37	126
MsG0480023039.01.T01	MTR_7g014880	42.336	137	73	2	15	147	10	144	1.26e-35	122
MsG0480023039.01.T01	MTR_1g103450	30.128	156	95	4	9	157	6	154	2.73e-13	63.9
MsG0480023039.01.T01	MTR_1g103470	41.096	73	40	1	33	105	25	94	2.48e-12	61.6
MsG0480023039.01.T01	MTR_1g103490	39.024	82	48	2	33	113	25	105	2.20e-11	58.9
MsG0480023039.01.T01	MTR_5g006940	34.862	109	64	4	10	113	6	112	2.72e-11	59.7
MsG0480023039.01.T01	MTR_5g006950	29.104	134	80	4	15	147	12	131	7.12e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023039.01.T01	AT2G44300	44.444	144	78	1	6	147	3	146	1.31e-39	133
MsG0480023039.01.T01	AT2G44290	43.284	134	76	0	14	147	14	147	2.91e-39	132
MsG0480023039.01.T01	AT1G55260	45.255	137	73	2	10	145	50	185	3.23e-38	130
MsG0480023039.01.T01	AT1G55260	45.255	137	73	2	10	145	50	185	3.46e-38	130
MsG0480023039.01.T01	AT3G58550	42.308	130	73	1	8	135	10	139	2.68e-33	116
MsG0480023039.01.T01	AT3G22600	29.104	134	94	1	15	148	7	139	1.02e-14	68.2
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT2G48130	35.000	100	63	2	15	113	9	107	8.34e-14	65.9
MsG0480023039.01.T01	AT1G73890	36.471	85	54	0	29	113	23	107	3.90e-13	64.3
MsG0480023039.01.T01	AT4G14815	35.106	94	57	2	15	108	8	97	3.19e-12	61.2
MsG0480023039.01.T01	AT1G03103	32.967	91	57	1	15	105	8	94	1.24e-11	60.1

Find 39 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTAGCCTCATGTGATTT+AGG	0.238066	4:-80046748	None:intergenic
ACAATCTATGGTTGGAGATT+TGG	0.249161	4:-80046456	None:intergenic
TACAATCCTTTGGTAACTTA+AGG	0.268613	4:-80046586	None:intergenic
GACACCAGTGCAACAATCTA+TGG	0.281431	4:-80046468	None:intergenic
ACCAATACATTGAGTTATAT+TGG	0.304514	4:-80046618	None:intergenic
TTTATAGTTAAACCAAGGTT+TGG	0.345145	4:-80046551	None:intergenic
GGTCATCATGATCTTTGATA+AGG	0.380512	4:-80046529	None:intergenic
ACAATCCTTTGGTAACTTAA+GGG	0.380953	4:-80046585	None:intergenic
CGGTGCATTCTGCTTTGTCT+TGG	0.411232	4:-80046385	None:intergenic
GACAAACTCCTTACTTTGGC+TGG	0.412680	4:+80046407	MsG0480023039.01.T01:CDS
GTAGGTGAGTTACAATCCTT+TGG	0.416024	4:-80046596	None:intergenic
TTATAGTTAAACCAAGGTTT+GGG	0.422627	4:-80046550	None:intergenic
ACCAATATAACTCAATGTAT+TGG	0.457228	4:+80046617	MsG0480023039.01.T01:CDS
CACCGACAAACTCCTTACTT+TGG	0.466376	4:+80046403	MsG0480023039.01.T01:CDS
GAAGACAACCAGCCAAAGTA+AGG	0.470397	4:-80046415	None:intergenic
ACATGAGGCTAAGGTATTTG+AGG	0.480429	4:+80046756	MsG0480023039.01.T01:CDS
ACCTAAATCACATGAGGCTA+AGG	0.495814	4:+80046747	MsG0480023039.01.T01:CDS
TTTGTCTTGGTTAATATCTG+AGG	0.495906	4:-80046372	None:intergenic
ACTTCCACCGCAGTTCCTCC+AGG	0.529242	4:+80046805	MsG0480023039.01.T01:CDS
CATAAGGACTAGGAAAATCA+AGG	0.531221	4:-80046336	None:intergenic
GATCATGATGACCCAAACCT+TGG	0.532203	4:+80046539	MsG0480023039.01.T01:CDS
TTGGCTTGATTAGTCACAAA+GGG	0.535509	4:-80046437	None:intergenic
CTTTGGTAACTTAAGGGCAA+GGG	0.546332	4:-80046579	None:intergenic
CCTTTGGTAACTTAAGGGCA+AGG	0.546657	4:-80046580	None:intergenic
AGGTTACAAGACCAAACATA+AGG	0.547469	4:-80046352	None:intergenic
CCAACCATAGATTGTTGCAC+TGG	0.548725	4:+80046464	MsG0480023039.01.T01:CDS
AATAGTAAATAAAATTTACC+TGG	0.560912	4:-80046823	None:intergenic
CCTTGCCCTTAAGTTACCAA+AGG	0.561963	4:+80046580	MsG0480023039.01.T01:CDS
CAAGACCAAACATAAGGACT+AGG	0.566810	4:-80046346	None:intergenic
AGCCAAAGTAAGGAGTTTGT+CGG	0.567852	4:-80046405	None:intergenic
ATACATTGAGTTATATTGGT+AGG	0.586657	4:-80046614	None:intergenic
CCAGTGCAACAATCTATGGT+TGG	0.604106	4:-80046464	None:intergenic
TAACATTTATAGTTAAACCA+AGG	0.621795	4:-80046556	None:intergenic
TTTGGCTTGATTAGTCACAA+AGG	0.627914	4:-80046438	None:intergenic
TTTGAAACCTAAATCACATG+AGG	0.643872	4:+80046741	MsG0480023039.01.T01:CDS
TTTACCTGGAGGAACTGCGG+TGG	0.660595	4:-80046809	None:intergenic
AGTAAATAAAATTTACCTGG+AGG	0.663286	4:-80046820	None:intergenic
AAATTTACCTGGAGGAACTG+CGG	0.666666	4:-80046812	None:intergenic
TTGTTGCACTGGTGTCAAAG+AGG	0.679195	4:+80046475	MsG0480023039.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGTAAATAAAATTTACC+TGG	-	Chr4:80046826-80046845	None:intergenic	15.0%
!!	TAACATTTATAGTTAAACCA+AGG	-	Chr4:80046559-80046578	None:intergenic	20.0%
!!!	TGAAACAAATATCAAACTTT+TGG	-	Chr4:80046710-80046729	None:intergenic	20.0%
!	ACCAATACATTGAGTTATAT+TGG	-	Chr4:80046621-80046640	None:intergenic	25.0%
!	ACCAATATAACTCAATGTAT+TGG	+	Chr4:80046617-80046636	MsG0480023039.01.T01:CDS	25.0%
!	AGTAAATAAAATTTACCTGG+AGG	-	Chr4:80046823-80046842	None:intergenic	25.0%
!	ATACATTGAGTTATATTGGT+AGG	-	Chr4:80046617-80046636	None:intergenic	25.0%
!	TTATAGTTAAACCAAGGTTT+GGG	-	Chr4:80046553-80046572	None:intergenic	25.0%
!	TTTATAGTTAAACCAAGGTT+TGG	-	Chr4:80046554-80046573	None:intergenic	25.0%
!!	TATTTTTCTCCAAGGATTTT+TGG	-	Chr4:80046786-80046805	None:intergenic	25.0%
	TTTGTCTTGGTTAATATCTG+AGG	-	Chr4:80046375-80046394	None:intergenic	30.0%
!	ACAATCCTTTGGTAACTTAA+GGG	-	Chr4:80046588-80046607	None:intergenic	30.0%
!	ATTTTCCTAGTCCTTATGTT+TGG	+	Chr4:80046341-80046360	MsG0480023039.01.T01:CDS	30.0%
!	TACAATCCTTTGGTAACTTA+AGG	-	Chr4:80046589-80046608	None:intergenic	30.0%
!	TTTGAAACCTAAATCACATG+AGG	+	Chr4:80046741-80046760	MsG0480023039.01.T01:CDS	30.0%
	ACAATCTATGGTTGGAGATT+TGG	-	Chr4:80046459-80046478	None:intergenic	35.0%
	AGGTTACAAGACCAAACATA+AGG	-	Chr4:80046355-80046374	None:intergenic	35.0%
	CATAAGGACTAGGAAAATCA+AGG	-	Chr4:80046339-80046358	None:intergenic	35.0%
	GGTCATCATGATCTTTGATA+AGG	-	Chr4:80046532-80046551	None:intergenic	35.0%
!	TTGGCTTGATTAGTCACAAA+GGG	-	Chr4:80046440-80046459	None:intergenic	35.0%
!!	TTTGGCTTGATTAGTCACAA+AGG	-	Chr4:80046441-80046460	None:intergenic	35.0%
	AAATTTACCTGGAGGAACTG+CGG	-	Chr4:80046815-80046834	None:intergenic	40.0%
	ACCTAAATCACATGAGGCTA+AGG	+	Chr4:80046747-80046766	MsG0480023039.01.T01:CDS	40.0%
	ACCTTAGCCTCATGTGATTT+AGG	-	Chr4:80046751-80046770	None:intergenic	40.0%
	AGCCAAAGTAAGGAGTTTGT+CGG	-	Chr4:80046408-80046427	None:intergenic	40.0%
	CAAGACCAAACATAAGGACT+AGG	-	Chr4:80046349-80046368	None:intergenic	40.0%
	GTAGGTGAGTTACAATCCTT+TGG	-	Chr4:80046599-80046618	None:intergenic	40.0%
	TGAGGACTTCCAAAAATCCT+TGG	+	Chr4:80046774-80046793	MsG0480023039.01.T01:CDS	40.0%
!	ACATGAGGCTAAGGTATTTG+AGG	+	Chr4:80046756-80046775	MsG0480023039.01.T01:CDS	40.0%
!	CTTTGGTAACTTAAGGGCAA+GGG	-	Chr4:80046582-80046601	None:intergenic	40.0%
!!!	GGTGGAAGTATTTTTCTCCA+AGG	-	Chr4:80046794-80046813	None:intergenic	40.0%
	CACCGACAAACTCCTTACTT+TGG	+	Chr4:80046403-80046422	MsG0480023039.01.T01:CDS	45.0%
	CCAACCATAGATTGTTGCAC+TGG	+	Chr4:80046464-80046483	MsG0480023039.01.T01:CDS	45.0%
	CCAGTGCAACAATCTATGGT+TGG	-	Chr4:80046467-80046486	None:intergenic	45.0%
	CCTTGCCCTTAAGTTACCAA+AGG	+	Chr4:80046580-80046599	MsG0480023039.01.T01:CDS	45.0%
	GAAGACAACCAGCCAAAGTA+AGG	-	Chr4:80046418-80046437	None:intergenic	45.0%
	GACAAACTCCTTACTTTGGC+TGG	+	Chr4:80046407-80046426	MsG0480023039.01.T01:CDS	45.0%
	GACACCAGTGCAACAATCTA+TGG	-	Chr4:80046471-80046490	None:intergenic	45.0%
	GATCATGATGACCCAAACCT+TGG	+	Chr4:80046539-80046558	MsG0480023039.01.T01:CDS	45.0%
!	CCTTTGGTAACTTAAGGGCA+AGG	-	Chr4:80046583-80046602	None:intergenic	45.0%
!	TTGTTGCACTGGTGTCAAAG+AGG	+	Chr4:80046475-80046494	MsG0480023039.01.T01:CDS	45.0%
	CGGTGCATTCTGCTTTGTCT+TGG	-	Chr4:80046388-80046407	None:intergenic	50.0%
	TTTACCTGGAGGAACTGCGG+TGG	-	Chr4:80046812-80046831	None:intergenic	55.0%
	ACTTCCACCGCAGTTCCTCC+AGG	+	Chr4:80046805-80046824	MsG0480023039.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	80046296	80046861	80046296	ID=MsG0480023039.01;Name=MsG0480023039.01
Chr4	mRNA	80046296	80046861	80046296	ID=MsG0480023039.01.T01;Parent=MsG0480023039.01;Name=MsG0480023039.01.T01;_AED=0.44;_eAED=0.48;_QI=0\|0\|0\|1\|1\|1\|2\|0\|157
Chr4	exon	80046296	80046638	80046296	ID=MsG0480023039.01.T01:exon:11452;Parent=MsG0480023039.01.T01
Chr4	exon	80046731	80046861	80046731	ID=MsG0480023039.01.T01:exon:11453;Parent=MsG0480023039.01.T01
Chr4	CDS	80046296	80046638	80046296	ID=MsG0480023039.01.T01:cds;Parent=MsG0480023039.01.T01
Chr4	CDS	80046731	80046861	80046731	ID=MsG0480023039.01.T01:cds;Parent=MsG0480023039.01.T01

Gene Sequence

>MsG0480023039.01.T01

ATGGGTTGCAAAAGCACAACAAATCTATCACTCTTCTCTACCTTGATTTTCCTAGTCCTTATGTTTGGTCTTGTAACCTCAGATATTAACCAAGACAAAGCAGAATGCACCGACAAACTCCTTACTTTGGCTGGTTGTCTTCCCTTTGTGACTAATCAAGCCAAATCTCCAACCATAGATTGTTGCACTGGTGTCAAAGAGGTTGTTGATAAGAGTAAGAGATGTTTATGTATCCTTATCAAAGATCATGATGACCCAAACCTTGGTTTAACTATAAATGTTACCCTTGCCCTTAAGTTACCAAAGGATTGTAACTCACCTACCAATATAACTCAATGTATTGATATTTTGCATTTGAAACCTAAATCACATGAGGCTAAGGTATTTGAGGACTTCCAAAAATCCTTGGAGAAAAATACTTCCACCGCAGTTCCTCCAGGTAAATTTTATTTACTATTTTTTAATCTTAAATAA

Protein sequence

>MsG0480023039.01.T01

MGCKSTTNLSLFSTLIFLVLMFGLVTSDINQDKAECTDKLLTLAGCLPFVTNQAKSPTIDCCTGVKEVVDKSKRCLCILIKDHDDPNLGLTINVTLALKLPKDCNSPTNITQCIDILHLKPKSHEAKVFEDFQKSLEKNTSTAVPPGKFYLLFFNLK*