AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016771.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016771.01.T01 MTR_3g095180 95.588 68 1 1 1 66 18 85 3.16e-43 134
MsG0380016771.01.T01 MTR_8g092340 54.545 66 24 3 1 66 18 77 3.82e-15 63.5
MsG0380016771.01.T02 MTR_3g095180 100.000 31 0 0 1 31 55 85 1.30e-18 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAATAGTGCACAAATTT+TGG 0.112038 3:+87734310 MsG0380016771.01.T01:exon
AGGTACCTTTAACTTGAATA+TGG 0.246398 3:-87734004 None:intergenic
TGCACTATTTAATTGTGAAT+TGG 0.396593 3:-87734300 None:intergenic
ATTGGTGGTTTCCTTGATGC+TGG 0.439868 3:-87734282 None:intergenic
TGTTGACATCCTCACTACTT+TGG 0.458998 3:-87734164 None:intergenic
CAACAAAGCTCAACAACATA+AGG 0.461599 3:+87734182 MsG0380016771.01.T01:CDS
ACCTTTAACTTGAATATGGA+TGG 0.490545 3:-87734000 None:intergenic
ACTATTTAATTGTGAATTGG+TGG 0.515565 3:-87734297 None:intergenic
GACATGTTTAAGCTAAAAGA+AGG 0.527331 3:+87734216 MsG0380016771.01.T01:CDS
AAATAGCTATTCTATAGCTA+TGG 0.532562 3:+87734376 MsG0380016771.01.T01:three_prime_UTR
ATATGGATGGAGAAGAAGCA+AGG 0.572438 3:-87733987 None:intergenic
TGGATTATACTCCAGCATCA+AGG 0.596772 3:+87734271 MsG0380016771.01.T01:CDS
AATAAACAACCAAAGTAGTG+AGG 0.645718 3:+87734155 MsG0380016771.01.T01:CDS
CAAAGCTCAACAACATAAGG+AGG 0.647677 3:+87734185 MsG0380016771.01.T01:CDS
ATGTTTAAGCTAAAAGAAGG+AGG 0.667536 3:+87734219 MsG0380016771.01.T01:CDS
AAAGAAGGAGGAGATAACGT+TGG 0.669136 3:+87734231 MsG0380016771.01.T01:CDS
TGATGCTGGAGTATAATCCA+TGG 0.719915 3:-87734268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATATTACAACAT+TGG + Chr3:87734049-87734068 MsG0380016771.01.T01:intron 10.0%
!! AATTAAACAGAAAATAAAAA+TGG - Chr3:87734088-87734107 None:intergenic 10.0%
!!! AATTTTTCTTACATTTTTTT+TGG + Chr3:87734108-87734127 MsG0380016771.01.T01:intron 10.0%
!!! GTAATATTTTTTTTTAGCTA+TGG - Chr3:87734044-87734063 None:intergenic 15.0%
!!! ATTAAATAGTGCACAAATTT+TGG + Chr3:87734310-87734329 MsG0380016771.01.T01:exon 20.0%
! AAATAGCTATTCTATAGCTA+TGG + Chr3:87734376-87734395 MsG0380016771.01.T01:three_prime_UTR 25.0%
! ACTATTTAATTGTGAATTGG+TGG - Chr3:87734300-87734319 None:intergenic 25.0%
! TGCACTATTTAATTGTGAAT+TGG - Chr3:87734303-87734322 None:intergenic 25.0%
AATAAACAACCAAAGTAGTG+AGG + Chr3:87734155-87734174 MsG0380016771.01.T01:CDS 30.0%
ATGTTTAAGCTAAAAGAAGG+AGG + Chr3:87734219-87734238 MsG0380016771.01.T01:CDS 30.0%
GACATGTTTAAGCTAAAAGA+AGG + Chr3:87734216-87734235 MsG0380016771.01.T01:CDS 30.0%
! CTATGGATATTTGAAGCAAA+AGG - Chr3:87734027-87734046 None:intergenic 30.0%
! TCCATCCATATTCAAGTTAA+AGG + Chr3:87733999-87734018 MsG0380016771.01.T01:intron 30.0%
!! ACCTTTAACTTGAATATGGA+TGG - Chr3:87734003-87734022 None:intergenic 30.0%
!! AGGTACCTTTAACTTGAATA+TGG - Chr3:87734007-87734026 None:intergenic 30.0%
CAACAAAGCTCAACAACATA+AGG + Chr3:87734182-87734201 MsG0380016771.01.T01:CDS 35.0%
AAAGAAGGAGGAGATAACGT+TGG + Chr3:87734231-87734250 MsG0380016771.01.T01:CDS 40.0%
CAAAGCTCAACAACATAAGG+AGG + Chr3:87734185-87734204 MsG0380016771.01.T01:CDS 40.0%
TGATGCTGGAGTATAATCCA+TGG - Chr3:87734271-87734290 None:intergenic 40.0%
TGGATTATACTCCAGCATCA+AGG + Chr3:87734271-87734290 MsG0380016771.01.T01:CDS 40.0%
TGTTGACATCCTCACTACTT+TGG - Chr3:87734167-87734186 None:intergenic 40.0%
!!! TGGTTCTGATGTTTTCACCA+TGG + Chr3:87734251-87734270 MsG0380016771.01.T01:CDS 40.0%
! ATTGGTGGTTTCCTTGATGC+TGG - Chr3:87734285-87734304 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 87733990 87734450 87733990 ID=MsG0380016771.01;Name=MsG0380016771.01
Chr3 mRNA 87733990 87734450 87733990 ID=MsG0380016771.01.T01;Parent=MsG0380016771.01;Name=MsG0380016771.01.T01;_AED=0.38;_eAED=0.38;_QI=0|0|0.5|1|0|0|2|136|66
Chr3 exon 87733990 87734014 87733990 ID=MsG0380016771.01.T01:exon:22271;Parent=MsG0380016771.01.T01
Chr3 exon 87734139 87734450 87734139 ID=MsG0380016771.01.T01:exon:22272;Parent=MsG0380016771.01.T01
Chr3 CDS 87733990 87734014 87733990 ID=MsG0380016771.01.T01:cds;Parent=MsG0380016771.01.T01
Chr3 CDS 87734139 87734314 87734139 ID=MsG0380016771.01.T01:cds;Parent=MsG0380016771.01.T01
Chr3 three_prime_UTR 87734315 87734450 87734315 ID=MsG0380016771.01.T01:three_prime_utr;Parent=MsG0380016771.01.T01
Chr3 mRNA 87734219 87734314 87734219 ID=MsG0380016771.01.T02;Parent=MsG0380016771.01;Name=MsG0380016771.01.T02;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|31
Chr3 exon 87734219 87734314 87734219 ID=MsG0380016771.01.T02:exon:22273;Parent=MsG0380016771.01.T02
Chr3 CDS 87734219 87734314 87734219 ID=MsG0380016771.01.T02:cds;Parent=MsG0380016771.01.T02
Gene Sequence

>MsG0380016771.01.T01

TGCTTCTTCTCCATCCATATTCAAGCAAGAGTATTGAAAGAAATAAACAACCAAAGTAGTGAGGATGTCAACAAAGCTCAACAACATAAGGAGGTACATGCTGACATGTTTAAGCTAAAAGAAGGAGGAGATAACGTTGGTTCTGATGTTTTCACCATGGATTATACTCCAGCATCAAGGAAACCACCAATTCACAATTAA

>MsG0380016771.01.T02

ATGTTTAAGCTAAAAGAAGGAGGAGATAACGTTGGTTCTGATGTTTTCACCATGGATTATACTCCAGCATCAAGGAAACCACCAATTCACAATTAA

Protein sequence

>MsG0380016771.01.T01

CFFSIHIQARVLKEINNQSSEDVNKAQQHKEVHADMFKLKEGGDNVGSDVFTMDYTPASRKPPIHN*

>MsG0380016771.01.T02

MFKLKEGGDNVGSDVFTMDYTPASRKPPIHN*