AlfalfaGEDB | MsG0580025502.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025502.01

KEGG: K05658
GO: GO:0005524 GO:0016021 GO:0016887 GO:0042626 GO:0055085
PFAM: PF00005 PF00664

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025502.01.T01	XP_003612850.1	99.028	1234	12	0	1	1234	1	1234	0	2496

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025502.01.T01	Q8LPK2	79.251	1229	251	1	3	1231	48	1272	0	2023

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025502.01.T01	G7KDP1	99.028	1234	12	0	1	1234	1	1234	0.0	2496

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047883.01	MsG0580025502.01	0.813279	2.773443e-51	1.803357e-48
MsG0080047884.01	MsG0580025502.01	0.807620	4.625531e-50	2.588503e-47
MsG0080047899.01	MsG0580025502.01	0.821861	3.223042e-53	2.653813e-50
MsG0080048528.01	MsG0580025502.01	0.813115	3.013561e-51	1.950611e-48
MsG0080048727.01	MsG0580025502.01	0.879405	1.280378e-69	6.933648e-66
MsG0080048748.01	MsG0580025502.01	0.850368	1.776716e-60	3.472240e-57
MsG0080048773.01	MsG0580025502.01	0.890491	9.393742e-74	7.914295e-70
MsG0080048777.01	MsG0580025502.01	0.826231	3.039196e-54	2.833489e-51
MsG0080048780.01	MsG0580025502.01	0.859080	5.296100e-63	1.381430e-59
MsG0080048801.01	MsG0580025502.01	0.881143	3.065267e-70	1.775320e-66

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PPI

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Gene1	Gene2	Type
MsG0180004752.01	MsG0580025502.01	PPI
MsG0380014209.01	MsG0580025502.01	PPI
MsG0580025502.01	MsG0680030679.01	PPI
MsG0580025502.01	MsG0880043598.01	PPI
MsG0580025502.01	MsG0680030656.01	PPI
MsG0580025502.01	MsG0180004753.01	PPI
MsG0580025502.01	MsG0680030746.01	PPI
MsG0580025502.01	MsG0680030677.01	PPI
MsG0680030672.01	MsG0580025502.01	PPI
MsG0680030679.01	MsG0580025502.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025502.01.T01	MTR_5g029750	99.028	1234	12	0	1	1234	1	1234	0.0	2496
MsG0580025502.01.T01	MTR_6g011680	51.616	1238	581	7	8	1230	15	1249	0.0	1329
MsG0580025502.01.T01	MTR_7g102070	50.080	1252	597	8	4	1228	71	1321	0.0	1253
MsG0580025502.01.T01	MTR_1g063170	49.406	1263	594	11	6	1233	48	1300	0.0	1217
MsG0580025502.01.T01	MTR_1g063170	41.753	582	320	9	665	1234	71	645	5.90e-129	429
MsG0580025502.01.T01	MTR_8g066710	78.996	757	147	3	476	1229	7	754	0.0	1199
MsG0580025502.01.T01	MTR_8g066710	39.286	588	344	6	11	594	174	752	3.29e-121	394
MsG0580025502.01.T01	MTR_1g025560	48.875	1244	603	9	2	1233	16	1238	0.0	1174
MsG0580025502.01.T01	MTR_2g018350	46.117	1249	644	9	5	1227	10	1255	0.0	1103
MsG0580025502.01.T01	MTR_2g018350	40.572	594	339	7	645	1228	9	598	2.55e-127	423

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025502.01.T01	AT4G25960	79.251	1229	251	1	3	1231	48	1272	0.0	2023
MsG0580025502.01.T01	AT4G25960	42.632	570	300	9	670	1233	97	645	3.67e-129	428
MsG0580025502.01.T01	AT1G10680	75.345	1233	276	4	7	1233	16	1226	0.0	1929
MsG0580025502.01.T01	AT1G10680	75.535	1169	258	4	7	1169	16	1162	0.0	1825
MsG0580025502.01.T01	AT1G10680	41.053	570	312	8	671	1228	43	600	1.15e-128	425
MsG0580025502.01.T01	AT3G28860	51.454	1238	583	7	8	1230	17	1251	0.0	1337
MsG0580025502.01.T01	AT1G27940	50.529	1229	578	9	15	1228	30	1243	0.0	1254
MsG0580025502.01.T01	AT1G28010	50.778	1221	588	8	15	1228	31	1245	0.0	1231
MsG0580025502.01.T01	AT1G28010	37.801	582	335	7	28	596	679	1246	6.53e-118	397
MsG0580025502.01.T01	AT2G36910	50.643	1244	588	8	15	1233	27	1269	0.0	1216

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Find 238 sgRNAs with CRISPR-Local

Find 558 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTGCTGTGAAGCTTT+CGG	0.436110	5:+19691163	MsG0580025502.01.T01:CDS
AAAATTGCTTTCCTCTTCTG+TGG	0.515212	5:+19692272	MsG0580025502.01.T01:CDS
AAAATTGTAGCACTTGTGGG+AGG	0.686950	5:+19690924	MsG0580025502.01.T01:CDS
AAAGAAAACAGCTTTAACAT+AGG	0.510706	5:-19685384	None:intergenic
AAAGACTCGATTGGCCGTGT+TGG	0.577219	5:+19692050	MsG0580025502.01.T01:CDS
AAATCTCTTAGAAAACACAT+TGG	0.667146	5:+19694783	MsG0580025502.01.T01:CDS
AAATGGCTTAGGCAACAAAT+TGG	0.366902	5:+19691053	MsG0580025502.01.T01:CDS
AACAGAAGCGCCATGAACAA+TGG	0.586106	5:-19685454	None:intergenic
AACATGGGGAAGATAAGTAT+GGG	0.618900	5:-19685142	None:intergenic
AACATTCTTTATGGAAAGGA+AGG	0.502763	5:+19694852	MsG0580025502.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAGATGTTCAATGGCA+TGG	-	Chr5:19692322-19692341	None:intergenic	30.0%
AACATTATCACAAGCAACAA+AGG	-	Chr5:19691309-19691328	None:intergenic	30.0%
AACATTCTTTATGGAAAGGA+AGG	+	Chr5:19694852-19694871	MsG0580025502.01.T01:CDS	30.0%
AAGTAGCTTATAACAAATGG+TGG	+	Chr5:19693717-19693736	MsG0580025502.01.T01:intron	30.0%
AATCTCATAAGTACCTTGAT+TGG	-	Chr5:19691789-19691808	None:intergenic	30.0%
AATCTTCAACAACCTAAATC+TGG	+	Chr5:19690887-19690906	MsG0580025502.01.T01:CDS	30.0%
AGCAAAAAAAGCTCGAAAAT+CGG	+	Chr5:19690786-19690805	MsG0580025502.01.T01:CDS	30.0%
ATAGTATATACCATAAGGCA+AGG	-	Chr5:19693421-19693440	None:intergenic	30.0%
ATCAAGGAGAATATCTTGTA+TGG	+	Chr5:19691113-19691132	MsG0580025502.01.T01:CDS	30.0%
ATGGAAAGATTATTGAGCAA+GGG	+	Chr5:19695201-19695220	MsG0580025502.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	19685139	19695573	19685139	ID=MsG0580025502.01;Name=MsG0580025502.01
Chr5	mRNA	19685139	19695573	19685139	ID=MsG0580025502.01.T01;Parent=MsG0580025502.01;Name=MsG0580025502.01.T01;_AED=0.47;_eAED=0.47;_QI=200\|1\|1\|1\|1\|1\|12\|266\|1234
Chr5	exon	19685139	19685566	19685139	ID=MsG0580025502.01.T01:exon:8288;Parent=MsG0580025502.01.T01
Chr5	exon	19685892	19685946	19685892	ID=MsG0580025502.01.T01:exon:8289;Parent=MsG0580025502.01.T01
Chr5	exon	19686072	19686247	19686072	ID=MsG0580025502.01.T01:exon:8290;Parent=MsG0580025502.01.T01
Chr5	exon	19689322	19689522	19689322	ID=MsG0580025502.01.T01:exon:8291;Parent=MsG0580025502.01.T01
Chr5	exon	19689786	19690045	19689786	ID=MsG0580025502.01.T01:exon:8292;Parent=MsG0580025502.01.T01
Chr5	exon	19690692	19691232	19690692	ID=MsG0580025502.01.T01:exon:8293;Parent=MsG0580025502.01.T01
Chr5	exon	19691415	19691794	19691415	ID=MsG0580025502.01.T01:exon:8294;Parent=MsG0580025502.01.T01
Chr5	exon	19691986	19692395	19691986	ID=MsG0580025502.01.T01:exon:8295;Parent=MsG0580025502.01.T01
Chr5	exon	19692613	19692854	19692613	ID=MsG0580025502.01.T01:exon:8296;Parent=MsG0580025502.01.T01
Chr5	exon	19693182	19693429	19693182	ID=MsG0580025502.01.T01:exon:8297;Parent=MsG0580025502.01.T01
Chr5	exon	19693764	19694176	19693764	ID=MsG0580025502.01.T01:exon:8298;Parent=MsG0580025502.01.T01
Chr5	exon	19694757	19695573	19694757	ID=MsG0580025502.01.T01:exon:8299;Parent=MsG0580025502.01.T01
Chr5	five_prime_UTR	19685139	19685338	19685139	ID=MsG0580025502.01.T01:five_prime_utr;Parent=MsG0580025502.01.T01
Chr5	CDS	19685339	19685566	19685339	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19685892	19685946	19685892	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19686072	19686247	19686072	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19689322	19689522	19689322	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19689786	19690045	19689786	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19690692	19691232	19690692	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19691415	19691794	19691415	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19691986	19692395	19691986	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19692613	19692854	19692613	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19693182	19693429	19693182	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19693764	19694176	19693764	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	CDS	19694757	19695307	19694757	ID=MsG0580025502.01.T01:cds;Parent=MsG0580025502.01.T01
Chr5	three_prime_UTR	19695308	19695573	19695308	ID=MsG0580025502.01.T01:three_prime_utr;Parent=MsG0580025502.01.T01

Gene Sequence

>MsG0580025502.01.T01

ATGGAATCTAAAGAAGGTGATGAGAGAAAAAAAGAACACAAAGTTCCTATGTTAAAGCTGTTTTCTTTTGCAGACTCTTATGATTATATCTTAATGTTTATTGGATCTATTGGAGCCATTGTTCATGGCGCTTCTGTTCCTATTTTCTTTATTTTCTTTGGAAAGTTGATCAATGTTATTGGCTTGGCTTATCTTTTCCCTAAGGAAGCCTCTCACAAAGTTGCCAAGTACTCATTGGATTTTGTGTATCTAAGTGTAGCAATATTATTTTCATCTTGGACAGAGGTGGCTTGTTGGATGCATACTGGAGAAAGACAAGCAGCAAAGATGAGAATGGCTTATTTAAAGTCTATGTTAAATCAAGATATAAGTTTATTTGATACTGAGGCATCCACTGGAGAGGTCATTTCTGCTATCACAAGTGACATTATTATTGTTCAAGATGCTCTATCAGAGAAGGTAGGGAACTTCTTGCACTACATTAGCCGATTCATTGCTGGATTTACAATTGGATTTGTGAGAGTGTGGCAGATTAGTTTAGTGACACTTTCAATTGTCCCAGCGATTGCCCTTGCTGGAGGTTGTTATGCATATGTTACTATTGGCCTTATTGCAAAAGTTAGAAAAGCATATGTCAGGGCTGGTGAAATTGCTGAAGAGGTCATAGGCAATGTTAGAACAGTACAAGCATTTGCAGGAGAAGAAAGAGCAGTAAAATCATATAAAGCAGCTCTTATGAAAACTTACGTAAACGGTAGAAAAGCAGGATTAGCAAAGGGTCTTGGACTTGGCTCAATGCATTGTGTTCTGTTTCTATCTTGGGCTTTACTTGTTTGGTACACTAGCGTTGTTGTTCACAAGAACATTGCCAATGGTGGCGAGTCATTCACTACCATGCTCAACGTCGTCATCTCTGGCCTGTCACTTGGGCAGGCAGCACCAGATATCTCGGCGTTTATCCGAGCAAAGGCAGCGGCTTATCCCATTTTTGAGATGATAGAGAGGGATACAGTGAGCAAAAAAAGCTCGAAAATCGGTCGAAAATTAAGCAAATTGGATGGTCATATCCAGTTTAAGGATGTCTGTTTCAGTTATCCGTCTCGTCCAGATGTAGCAATCTTCAACAACCTAAATCTGGACATTCCTGCAGGGAAAATTGTAGCACTTGTGGGAGGAAGTGGTTCCGGAAAGAGCACTGTTGTATCGTTGATCGAACGTTTTTATGAACCAATTTCTGGTCAGATACTCTTAGACAAGAATGATATCAGGGAACTTGATCTCAAATGGCTTAGGCAACAAATTGGACTGGTTAATCAAGAACCTGCACTTTTTGCTACAAGCATCAAGGAGAATATCTTGTATGGGAAAGATGATGCTACTCTCGAAGAACTAAAACGTGCTGTGAAGCTTTCGGATGCTCAATCTTTCATCAACAACCTTCCCGAAAGATTAGACACTCAGGTTGGTGAGAGAGGGATACAACTATCAGGTGGACAGAAGCAAAGGATTGCGATATCTCGCGCGATTGTGAAGAATCCATCGATCCTTTTATTAGATGAAGCAACTAGTGCTTTGGATGCCGAGTCTGAGAAGAGTGTGCAGGAGGCTCTCGATCGTGTCATGGTTGGAAGAACAACTATTGTAGTTGCACACAGACTTTCTACCATTAGGAATGCAGATGTTATAGCTGTTGTTCAAGGAGGGAGGATTGTGGAGACCGGTAACCATGAAAAACTCATGTCCAATCCAACTAGTGTTTATGCATCTCTTGTTCAACTCCAAGGAGCAAGTTCTTTGCAACGCCTTCCCTCAGTCGGTCCTAGCTTAGGACGCCAATCAAGCTTAAGCTACTCAAGAGAATTATCACGTACTGGAACAAGCATCGGTGGTAGTTTTCGATCGGACAAAGACTCGATTGGCCGTGTTGGTGGTGATGATGTAAACAAGTCAAAGCATGTTTCAGCAAAAAGATTATATTCTATGATTCGTCCTGACTGGCCTTATGGATTTTTTGGGACCTTATGTGCATTTGTTGCTGGAGCACAAATGCCACTTTTTGCACTTGGAATCTCTCATGCTCTTGTCTCATATTATATGGATTGGGAGACAACGCAGCGCGAAGTTAGGAAAATTGCTTTCCTCTTCTGTGGAGGTGCAGTTATAACTATCACAGTCCATGCCATTGAACATCTTTTTTTTGGTATCATGGGCGAGCGACTTACCCTTCGTGTGCGAGAAATGATGTTTACCGCTATTTTGAAGAATGAAATAGGATGGTTTGATGAAACAACCAACACAAGTTCTATGCTTTCATCGCGTTTAGAGTCCGACGCAACTTTAATGCGAACAATTGTTGTTGATCGCTCGACGATTCTGTTACAGAATCTTGGTCTGGTTGTTGCTTCATTTATTATTGCCTTCCTTTTGAACTGGAGAATAACACTTGTTGTCTTGGCCACATATCCTTTGATAATTAGTGGTCACATTAGTGAGAAACTTTTCATGAAAGGCTATGGTGGCAACTTGAGCAAAGCATATCTAAAAGCCAACATGCTTGCTGGCGAGGCTGTGAGTAACATGCGCACTGTTGCCGCATTTTGTTCTGAAGAAAAGATTCTTGATCTATATGCTGACCAGCTCGTCGGTCCTTCCAAACATTCATTTCGGCGCGGCCAAATTGCCGGCTTATTCTATGGCATTTCTCAGTTCTTCATCTTCTCATCTTATGGCCTTGCCTTATGGTATGGATCTGTTTTGATGGGAAAGGAACTTGCTAGCTTTAAATCAGTTATGAAGTCATTTATGGTTTTGATTGTAACGGCACTTGCTATGGGGGAAACTTTGGCACTAGCACCAGATCTTTTGAAAGGGAACCAAATGGTTGCTTCAGTTTTTGAAGTAATGGATAGAAAGTCAGAAATTAAAGGCGATGCCGGAGAAGAGTTGAAGACAGTTGAGGGAACAATTGAGCTCAAAAGAATCAATTTCAGCTACCCTTCAAGGCCAGATGTTATTATTTTCAAGGATTTTAATCTAAGAGTTCCTTCAGGCAAAAGTGTTGCATTAGTGGGACAAAGTGGTTCTGGTAAAAGCTCTGTGATCTCACTTATACTCAGATTTTATGATCCAACATCAGGAAAGGTGTTAATTGATGGAAAAGACATCACAAGAATAAATCTAAAATCTCTTAGAAAACACATTGGTCTTGTACAACAAGAACCAGCTCTTTTCGCTACATCAATTTACGAAAACATTCTTTATGGAAAGGAAGGTGCATCTGATTCAGAGGTAATTGAAGCAGCTAAGCTAGCAAATGCTCATAACTTCATAAGTGCTCTTCCAGAAGGTTACTCAACCAAAGTAGGGGAAAGAGGTGTGCAACTATCTGGTGGACAAAGACAAAGGGTAGCCATTGCTAGAGCTGTTTTAAAGAATCCTGAAATCTTGCTTCTAGATGAAGCAACAAGTGCACTTGATGTCGAATCGGAGCGTATTGTTCAACAAGCTCTTGACCGATTGATGCAGAATAGAACAACAGTTATGGTGGCACATAGGTTGTCAACAATAAGGAATGCAGATCAAATCTCAGTTTTGCAAGATGGAAAGATTATTGAGCAAGGGACTCATTCAAGTCTTATAGAGAATAAAGATGGACCATATTATAAATTAGTCAACCTTCAACAACAACAAAATCATCAATCATAG

Protein sequence

>MsG0580025502.01.T01

MESKEGDERKKEHKVPMLKLFSFADSYDYILMFIGSIGAIVHGASVPIFFIFFGKLINVIGLAYLFPKEASHKVAKYSLDFVYLSVAILFSSWTEVACWMHTGERQAAKMRMAYLKSMLNQDISLFDTEASTGEVISAITSDIIIVQDALSEKVGNFLHYISRFIAGFTIGFVRVWQISLVTLSIVPAIALAGGCYAYVTIGLIAKVRKAYVRAGEIAEEVIGNVRTVQAFAGEERAVKSYKAALMKTYVNGRKAGLAKGLGLGSMHCVLFLSWALLVWYTSVVVHKNIANGGESFTTMLNVVISGLSLGQAAPDISAFIRAKAAAYPIFEMIERDTVSKKSSKIGRKLSKLDGHIQFKDVCFSYPSRPDVAIFNNLNLDIPAGKIVALVGGSGSGKSTVVSLIERFYEPISGQILLDKNDIRELDLKWLRQQIGLVNQEPALFATSIKENILYGKDDATLEELKRAVKLSDAQSFINNLPERLDTQVGERGIQLSGGQKQRIAISRAIVKNPSILLLDEATSALDAESEKSVQEALDRVMVGRTTIVVAHRLSTIRNADVIAVVQGGRIVETGNHEKLMSNPTSVYASLVQLQGASSLQRLPSVGPSLGRQSSLSYSRELSRTGTSIGGSFRSDKDSIGRVGGDDVNKSKHVSAKRLYSMIRPDWPYGFFGTLCAFVAGAQMPLFALGISHALVSYYMDWETTQREVRKIAFLFCGGAVITITVHAIEHLFFGIMGERLTLRVREMMFTAILKNEIGWFDETTNTSSMLSSRLESDATLMRTIVVDRSTILLQNLGLVVASFIIAFLLNWRITLVVLATYPLIISGHISEKLFMKGYGGNLSKAYLKANMLAGEAVSNMRTVAAFCSEEKILDLYADQLVGPSKHSFRRGQIAGLFYGISQFFIFSSYGLALWYGSVLMGKELASFKSVMKSFMVLIVTALAMGETLALAPDLLKGNQMVASVFEVMDRKSEIKGDAGEELKTVEGTIELKRINFSYPSRPDVIIFKDFNLRVPSGKSVALVGQSGSGKSSVISLILRFYDPTSGKVLIDGKDITRINLKSLRKHIGLVQQEPALFATSIYENILYGKEGASDSEVIEAAKLANAHNFISALPEGYSTKVGERGVQLSGGQRQRVAIARAVLKNPEILLLDEATSALDVESERIVQQALDRLMQNRTTVMVAHRLSTIRNADQISVLQDGKIIEQGTHSSLIENKDGPYYKLVNLQQQQNHQS*