AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029583.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029583.01.T01 MTR_5g088770 94.231 104 6 0 1 104 1 104 5.61e-67 196
MsG0580029583.01.T01 MTR_3g043750 62.000 100 35 1 1 100 1 97 8.32e-39 125
MsG0580029583.01.T01 MTR_8g041410 50.476 105 50 2 1 104 38 141 2.24e-31 110
MsG0580029583.01.T01 MTR_3g451210 46.000 100 51 1 4 103 31 127 1.90e-21 82.8
MsG0580029583.01.T01 MTR_3g452280 46.000 100 51 1 4 103 31 127 1.90e-21 82.8
MsG0580029583.01.T01 MTR_4g133540 37.647 85 52 1 5 89 29 112 1.55e-14 64.3
MsG0580029583.01.T01 MTR_0526s0020 42.466 73 42 0 19 91 28 100 9.84e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029583.01.T01 AT3G12490 54.808 104 44 1 1 104 1 101 2.39e-35 118
MsG0580029583.01.T01 AT3G12490 54.808 104 44 1 1 104 34 134 2.65e-34 118
MsG0580029583.01.T01 AT2G40880 51.163 86 42 0 4 89 35 120 2.06e-27 97.4
MsG0580029583.01.T01 AT5G05110 51.064 94 45 1 4 96 45 138 8.51e-27 99.0
MsG0580029583.01.T01 AT5G12140 47.126 87 46 0 4 90 10 96 3.25e-25 91.3
MsG0580029583.01.T01 AT4G16500 44.444 72 39 1 22 92 46 117 1.06e-13 62.4
MsG0580029583.01.T01 AT4G16500 44.444 72 39 1 22 92 46 117 1.06e-13 62.4

Find 33 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAGGAGCATCGTAATTT+TGG 0.195527 5:+98945598 None:intergenic
AAAGATAGAAATTAAATTAA+TGG 0.234510 5:-98947012 MsG0580029583.01.T01:exon
GCAAAGAAGCAAGTGGTTTC+TGG 0.255766 5:-98945741 MsG0580029583.01.T01:CDS
ATGATATCAAATAGTATATA+TGG 0.272791 5:-98945534 MsG0580029583.01.T01:three_prime_UTR
ATAGATATTGTGATCTATTC+AGG 0.278083 5:-98945424 MsG0580029583.01.T01:three_prime_UTR
CCATAGCTTCTCTAAAATCT+TGG 0.357443 5:+98945659 None:intergenic
AAATAGTATATATGGAATAA+TGG 0.365350 5:-98945526 MsG0580029583.01.T01:three_prime_UTR
CTTCAATTAAGCCTTATTGA+TGG 0.391380 5:-98945470 MsG0580029583.01.T01:three_prime_UTR
TCTGTATCTAGCACTTAGTT+AGG 0.401292 5:-98945579 MsG0580029583.01.T01:exon
AATAAACTAGACCATCAATA+AGG 0.412902 5:+98945459 None:intergenic
CACAATATCTATACCAGAAA+AGG 0.412941 5:+98945435 None:intergenic
AGTTAGGTATTTAAATTTCA+AGG 0.439742 5:-98945563 MsG0580029583.01.T01:three_prime_UTR
GAAGCTATGGTTGAACTTCA+AGG 0.441137 5:-98945646 MsG0580029583.01.T01:CDS
TCAAGAACACAATATCAAAC+AGG 0.464934 5:-98946913 MsG0580029583.01.T01:intron
CAAACATTGATCAATTGACT+TGG 0.471566 5:+98945390 None:intergenic
CTCTAAAGTGATGTCGTACA+AGG 0.516796 5:+98945716 None:intergenic
GTTGAACTTCAAGGAGGTGC+AGG 0.521993 5:-98945637 MsG0580029583.01.T01:CDS
ATTAAATTAATGGCGACTCT+AGG 0.533183 5:-98947002 MsG0580029583.01.T01:exon
AATGGATGGTGGGAAGCAAA+AGG 0.558878 5:-98945691 MsG0580029583.01.T01:CDS
CTTTAGAGACAATGGATGGT+GGG 0.567845 5:-98945701 MsG0580029583.01.T01:CDS
CGACATCACTTTAGAGACAA+TGG 0.568752 5:-98945709 MsG0580029583.01.T01:CDS
CTTGAACAGCAAAGATAGCA+AGG 0.570675 5:+98946931 None:intergenic
ATGCAGTTCTAGAGTTTGTG+AGG 0.589311 5:-98945773 MsG0580029583.01.T01:CDS
ATATTGTGATCTATTCAGGT+TGG 0.589671 5:-98945420 MsG0580029583.01.T01:three_prime_UTR
TCTAGGTGGTAGCACTGAGG+TGG 0.608936 5:-98946985 MsG0580029583.01.T01:CDS
ACTTTAGAGACAATGGATGG+TGG 0.617779 5:-98945702 MsG0580029583.01.T01:CDS
GTTAAATGCAAAGAAGCAAG+TGG 0.625710 5:-98945748 MsG0580029583.01.T01:CDS
AACTAAGTGCTAGATACAGA+AGG 0.629142 5:+98945582 None:intergenic
TGCAGTTCTAGAGTTTGTGA+GGG 0.629223 5:-98945772 MsG0580029583.01.T01:CDS
AAATTAATGGCGACTCTAGG+TGG 0.639281 5:-98946999 MsG0580029583.01.T01:exon
ATCACTTTAGAGACAATGGA+TGG 0.646105 5:-98945705 MsG0580029583.01.T01:CDS
GACTCTAGGTGGTAGCACTG+AGG 0.714011 5:-98946988 MsG0580029583.01.T01:CDS
GCTATGGTTGAACTTCAAGG+AGG 0.769675 5:-98945643 MsG0580029583.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG - Chr5:98946069-98946088 MsG0580029583.01.T01:intron 0.0%
!! AAAGATAGAAATTAAATTAA+TGG - Chr5:98945359-98945378 MsG0580029583.01.T01:three_prime_UTR 10.0%
!! AACTTATTCATAATTTATTA+GGG + Chr5:98946035-98946054 None:intergenic 10.0%
!! ATAAATGAACATTAAATAAA+TGG - Chr5:98945831-98945850 MsG0580029583.01.T01:intron 10.0%
!! TAACTTATTCATAATTTATT+AGG + Chr5:98946036-98946055 None:intergenic 10.0%
!! TTAATCTAACTAAAATTAAT+TGG - Chr5:98946518-98946537 MsG0580029583.01.T01:intron 10.0%
!! AAATAGTATATATGGAATAA+TGG - Chr5:98946845-98946864 MsG0580029583.01.T01:intron 15.0%
!! ACTTATTCATAATTTATTAG+GGG + Chr5:98946034-98946053 None:intergenic 15.0%
!! ATGAATAAGTTATTAACATT+TGG - Chr5:98946044-98946063 MsG0580029583.01.T01:intron 15.0%
!! ATGATATCAAATAGTATATA+TGG - Chr5:98946837-98946856 MsG0580029583.01.T01:intron 15.0%
!! TAATCATTAATATAACGATT+TGG + Chr5:98946191-98946210 None:intergenic 15.0%
!!! TTCTATACTATATTTTACAA+AGG + Chr5:98945557-98945576 None:intergenic 15.0%
!! ACTCAAATGCATATATATTA+TGG - Chr5:98946372-98946391 MsG0580029583.01.T01:intron 20.0%
!! AGTTAGGTATTTAAATTTCA+AGG - Chr5:98946808-98946827 MsG0580029583.01.T01:intron 20.0%
!! ATATATATAGGCAAATTCTA+TGG - Chr5:98946135-98946154 MsG0580029583.01.T01:intron 20.0%
!! TGAGATTCACTTATTAAAAA+GGG - Chr5:98945775-98945794 MsG0580029583.01.T01:CDS 20.0%
!! TTAATGTTTATCTCTTTACA+CGG + Chr5:98945745-98945764 None:intergenic 20.0%
! AATAAACTAGACCATCAATA+AGG + Chr5:98946915-98946934 None:intergenic 25.0%
! ATAGATATTGTGATCTATTC+AGG - Chr5:98946947-98946966 MsG0580029583.01.T01:CDS 25.0%
! GTGAGATTCACTTATTAAAA+AGG - Chr5:98945774-98945793 MsG0580029583.01.T01:CDS 25.0%
!! CTATATTTTACAAAGGAAAC+TGG + Chr5:98945550-98945569 None:intergenic 25.0%
!! TATCATTGTATTAGTCTAGA+AGG - Chr5:98945926-98945945 MsG0580029583.01.T01:intron 25.0%
!!! TTCAAAAGTACTAAGAGTTT+CGG + Chr5:98945669-98945688 None:intergenic 25.0%
AGGACAGAACAAAATTGAAA+TGG + Chr5:98945493-98945512 None:intergenic 30.0%
ATATTGTGATCTATTCAGGT+TGG - Chr5:98946951-98946970 MsG0580029583.01.T01:CDS 30.0%
ATTAAATTAATGGCGACTCT+AGG - Chr5:98945369-98945388 MsG0580029583.01.T01:three_prime_UTR 30.0%
CAAACATTGATCAATTGACT+TGG + Chr5:98946984-98947003 None:intergenic 30.0%
CACAATATCTATACCAGAAA+AGG + Chr5:98946939-98946958 None:intergenic 30.0%
TCAAGAACACAATATCAAAC+AGG - Chr5:98945458-98945477 MsG0580029583.01.T01:three_prime_UTR 30.0%
TTATCAAGCAAACAAAACAC+AGG + Chr5:98945513-98945532 None:intergenic 30.0%
!! AATATAACGATTTGGTGTAC+CGG + Chr5:98946183-98946202 None:intergenic 30.0%
!! CTTCAATTAAGCCTTATTGA+TGG - Chr5:98946901-98946920 MsG0580029583.01.T01:intron 30.0%
!! GTCTAGTTTATTACCTTTTC+TGG - Chr5:98946923-98946942 MsG0580029583.01.T01:CDS 30.0%
!! TTTTATTTCGTCGAAGCTTT+TGG + Chr5:98946282-98946301 None:intergenic 30.0%
!!! CGTTTTTTTTTCCTTTGTAC+CGG - Chr5:98945613-98945632 MsG0580029583.01.T01:CDS 30.0%
!!! GTTTTTTTTTCCTTTGTACC+GGG - Chr5:98945614-98945633 MsG0580029583.01.T01:CDS 30.0%
AAATCGATCATGAAATGACC+CGG + Chr5:98945635-98945654 None:intergenic 35.0%
AACTAAGTGCTAGATACAGA+AGG + Chr5:98946792-98946811 None:intergenic 35.0%
ACCCAAAAATTTGAGTGTAC+CGG - Chr5:98946161-98946180 MsG0580029583.01.T01:intron 35.0%
GTTAAATGCAAAGAAGCAAG+TGG - Chr5:98946623-98946642 MsG0580029583.01.T01:intron 35.0%
TCTGTATCTAGCACTTAGTT+AGG - Chr5:98946792-98946811 MsG0580029583.01.T01:intron 35.0%
! ACCGGTACACTCAAATTTTT+GGG + Chr5:98946165-98946184 None:intergenic 35.0%
! ATCACTTTAGAGACAATGGA+TGG - Chr5:98946666-98946685 MsG0580029583.01.T01:intron 35.0%
! TACCGGTACACTCAAATTTT+TGG + Chr5:98946166-98946185 None:intergenic 35.0%
!! CCAAGATTTTAGAGAAGCTA+TGG - Chr5:98946712-98946731 MsG0580029583.01.T01:intron 35.0%
!! CCATAGCTTCTCTAAAATCT+TGG + Chr5:98946715-98946734 None:intergenic 35.0%
AAATTAATGGCGACTCTAGG+TGG - Chr5:98945372-98945391 MsG0580029583.01.T01:three_prime_UTR 40.0%
CGTGACATAATAATGAGACG+TGG - Chr5:98946459-98946478 MsG0580029583.01.T01:intron 40.0%
CTTGAACAGCAAAGATAGCA+AGG + Chr5:98945443-98945462 None:intergenic 40.0%
GAAGCTATGGTTGAACTTCA+AGG - Chr5:98946725-98946744 MsG0580029583.01.T01:intron 40.0%
GTGAAGGAGTTATATGCAGT+AGG - Chr5:98945977-98945996 MsG0580029583.01.T01:intron 40.0%
TTCTGCGCATTTAAGCATCA+AGG + Chr5:98946579-98946598 None:intergenic 40.0%
! ACTTTAGAGACAATGGATGG+TGG - Chr5:98946669-98946688 MsG0580029583.01.T01:intron 40.0%
! CGACATCACTTTAGAGACAA+TGG - Chr5:98946662-98946681 MsG0580029583.01.T01:intron 40.0%
! CTTTAGAGACAATGGATGGT+GGG - Chr5:98946670-98946689 MsG0580029583.01.T01:intron 40.0%
!! AAGGAGTATCCAACTGGTTT+GGG - Chr5:98945945-98945964 MsG0580029583.01.T01:intron 40.0%
!! ATGCAGTTCTAGAGTTTGTG+AGG - Chr5:98946598-98946617 MsG0580029583.01.T01:intron 40.0%
!! CTCTAAAGTGATGTCGTACA+AGG + Chr5:98946658-98946677 None:intergenic 40.0%
!! TGCAGTTCTAGAGTTTGTGA+GGG - Chr5:98946599-98946618 MsG0580029583.01.T01:intron 40.0%
!!! CAGAAGGAGCATCGTAATTT+TGG + Chr5:98946776-98946795 None:intergenic 40.0%
CATGAAATGACCCGGTACAA+AGG + Chr5:98945627-98945646 None:intergenic 45.0%
GCAAAGAAGCAAGTGGTTTC+TGG - Chr5:98946630-98946649 MsG0580029583.01.T01:intron 45.0%
GCTATGGTTGAACTTCAAGG+AGG - Chr5:98946728-98946747 MsG0580029583.01.T01:intron 45.0%
!! AATGGATGGTGGGAAGCAAA+AGG - Chr5:98946680-98946699 MsG0580029583.01.T01:intron 45.0%
!! GAAGGAGTATCCAACTGGTT+TGG - Chr5:98945944-98945963 MsG0580029583.01.T01:intron 45.0%
!! GTCTAGAAGGAGTATCCAAC+TGG - Chr5:98945939-98945958 MsG0580029583.01.T01:intron 45.0%
GTGTTACAGCCCAAACCAGT+TGG + Chr5:98945957-98945976 None:intergenic 50.0%
GTTTGGGCTGTAACACGTGA+AGG - Chr5:98945961-98945980 MsG0580029583.01.T01:intron 50.0%
! GTTGAACTTCAAGGAGGTGC+AGG - Chr5:98946734-98946753 MsG0580029583.01.T01:intron 50.0%
GACTCTAGGTGGTAGCACTG+AGG - Chr5:98945383-98945402 MsG0580029583.01.T01:three_prime_UTR 55.0%
! TCTAGGTGGTAGCACTGAGG+TGG - Chr5:98945386-98945405 MsG0580029583.01.T01:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 98945350 98947043 98945350 ID=MsG0580029583.01;Name=MsG0580029583.01
Chr5 mRNA 98945350 98947043 98945350 ID=MsG0580029583.01.T01;Parent=MsG0580029583.01;Name=MsG0580029583.01.T01;_AED=0.35;_eAED=0.35;_QI=28|1|1|1|1|1|2|234|104
Chr5 exon 98945350 98945796 98945350 ID=MsG0580029583.01.T01:exon:25961;Parent=MsG0580029583.01.T01
Chr5 exon 98946914 98947043 98946914 ID=MsG0580029583.01.T01:exon:25960;Parent=MsG0580029583.01.T01
Chr5 five_prime_UTR 98947016 98947043 98947016 ID=MsG0580029583.01.T01:five_prime_utr;Parent=MsG0580029583.01.T01
Chr5 CDS 98946914 98947015 98946914 ID=MsG0580029583.01.T01:cds;Parent=MsG0580029583.01.T01
Chr5 CDS 98945584 98945796 98945584 ID=MsG0580029583.01.T01:cds;Parent=MsG0580029583.01.T01
Chr5 three_prime_UTR 98945350 98945583 98945350 ID=MsG0580029583.01.T01:three_prime_utr;Parent=MsG0580029583.01.T01
Gene Sequence

>MsG0580029583.01.T01

ATGGCGACTCTAGGTGGTAGCACTGAGGTGGAATATACACAAAACAGTGTTGAGATAGATAACCTTGCTATCTTTGCTGTTCAAGAACACAATATCAAACAGAATGCAGTTCTAGAGTTTGTGAGGGTGTTAAATGCAAAGAAGCAAGTGGTTTCTGGCACCTTGTACGACATCACTTTAGAGACAATGGATGGTGGGAAGCAAAAGGTGTATGAAGCCAAGATTTTAGAGAAGCTATGGTTGAACTTCAAGGAGGTGCAGGAATTCAATCTCATTAGCCAAAATTACGATGCTCCTTCTGTATCTAGCACTTAG

Protein sequence

>MsG0580029583.01.T01

MATLGGSTEVEYTQNSVEIDNLAIFAVQEHNIKQNAVLEFVRVLNAKKQVVSGTLYDITLETMDGGKQKVYEAKILEKLWLNFKEVQEFNLISQNYDAPSVSST*