AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043246.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043246.01.T01 MTR_8g031910 94.141 256 14 1 1 256 33 287 0.0 499
MsG0880043246.01.T01 MTR_7g076660 64.844 256 88 2 1 256 9 262 1.94e-119 342
MsG0880043246.01.T01 MTR_7g076660 64.844 256 88 2 1 256 33 286 2.38e-119 342
MsG0880043246.01.T01 MTR_7g076660 64.844 256 88 2 1 256 34 287 5.85e-119 341
MsG0880043246.01.T01 MTR_7g076655 64.173 254 89 2 3 256 35 286 3.36e-118 339
MsG0880043246.01.T01 MTR_8g031990 55.224 268 106 4 1 256 33 298 7.68e-102 298
MsG0880043246.01.T01 MTR_7g076665 54.924 264 110 4 1 256 33 295 1.01e-97 288
MsG0880043246.01.T01 MTR_8g032010 53.358 268 111 4 1 256 33 298 1.88e-97 287
MsG0880043246.01.T01 MTR_8g031960 56.226 265 102 5 1 253 34 296 5.60e-97 286
MsG0880043246.01.T01 MTR_8g031980 53.731 268 110 4 1 256 33 298 5.42e-96 283
MsG0880043246.01.T01 MTR_8g032000 52.985 268 112 4 1 256 33 298 9.14e-96 283
MsG0880043246.01.T01 MTR_8g032020 51.673 269 115 4 1 256 34 300 4.67e-92 273
MsG0880043246.01.T01 MTR_8g031630 53.731 268 109 6 1 256 33 297 3.32e-91 271
MsG0880043246.01.T01 MTR_8g031900 52.471 263 116 5 1 256 33 293 4.62e-91 271
MsG0880043246.01.T01 MTR_8g031610 53.933 267 109 6 1 256 31 294 5.49e-91 271
MsG0880043246.01.T01 MTR_8g031650 53.731 268 109 6 1 256 127 391 2.10e-89 270
MsG0880043246.01.T01 MTR_8g031650 58.586 99 39 2 127 223 2 100 7.20e-33 123
MsG0880043246.01.T01 MTR_8g031940 51.292 271 113 5 1 256 33 299 2.65e-89 266
MsG0880043246.01.T01 MTR_8g031600 52.239 268 114 4 1 256 33 298 6.42e-89 266
MsG0880043246.01.T01 MTR_8g031650 53.731 268 109 6 1 256 293 557 6.04e-88 271
MsG0880043246.01.T01 MTR_8g031650 54.043 235 95 5 1 223 33 266 4.65e-77 243
MsG0880043246.01.T01 MTR_5g062280 47.744 266 126 5 1 256 33 295 2.43e-81 246
MsG0880043246.01.T01 MTR_5g083430 58.382 173 71 1 84 256 13 184 6.10e-68 208
MsG0880043246.01.T01 MTR_8g031850 44.487 263 99 7 1 256 33 255 7.08e-67 208
MsG0880043246.01.T01 MTR_3g079320 44.906 265 125 6 1 247 37 298 3.96e-64 202
MsG0880043246.01.T01 MTR_7g106800 44.488 254 93 4 3 252 37 246 8.29e-64 201
MsG0880043246.01.T01 MTR_3g098470 42.489 233 108 7 31 251 88 306 7.52e-48 161
MsG0880043246.01.T01 MTR_1g031180 42.373 236 110 6 28 251 90 311 4.13e-44 151
MsG0880043246.01.T01 MTR_4g087460 33.333 270 153 7 1 256 48 304 1.12e-38 137
MsG0880043246.01.T01 MTR_8g032030 52.941 136 50 3 8 130 40 174 3.34e-37 131
MsG0880043246.01.T01 MTR_8g096060 38.983 236 115 8 28 251 87 305 5.67e-37 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043246.01.T01 AT3G61220 54.924 264 111 3 1 256 33 296 1.09e-98 290
MsG0880043246.01.T01 AT3G61220 54.924 264 111 3 1 256 40 303 3.33e-98 289
MsG0880043246.01.T01 AT3G61220 54.924 264 111 3 1 256 64 327 1.14e-97 289
MsG0880043246.01.T01 AT2G24190 54.086 257 110 3 8 256 40 296 1.40e-93 277
MsG0880043246.01.T01 AT2G24190 54.086 257 110 3 8 256 45 301 2.28e-93 277
MsG0880043246.01.T01 AT1G01800 52.632 266 115 4 1 256 31 295 1.64e-90 270
MsG0880043246.01.T01 AT1G01800 54.106 207 85 3 59 256 55 260 1.12e-69 215
MsG0880043246.01.T01 AT5G51030 39.583 240 118 8 28 254 88 313 6.30e-45 153
MsG0880043246.01.T01 AT5G61830 38.340 253 130 6 11 251 73 311 2.12e-43 149
MsG0880043246.01.T01 AT5G61830 38.340 253 130 6 11 251 73 311 2.12e-43 149
MsG0880043246.01.T01 AT3G59710 37.154 253 137 7 1 248 45 280 2.36e-43 149
MsG0880043246.01.T01 AT3G59710 37.154 253 137 7 1 248 55 290 3.18e-43 149
MsG0880043246.01.T01 AT3G59710 37.154 253 137 7 1 248 74 309 1.20e-42 147

Find 48 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACTGCAGGACTATTAA+AGG 0.190820 8:-21174354 MsG0880043246.01.T01:intron
AATTCTCTCCAATTAATAAT+TGG 0.203142 8:+21174500 None:intergenic
TCGAGTGATTCCACCACTTC+TGG 0.273816 8:-21173219 MsG0880043246.01.T01:CDS
AGGTGGAACCAATTATTAAT+TGG 0.289077 8:-21174508 MsG0880043246.01.T01:CDS
ACCAATGAAGCAATACTAAA+AGG 0.304929 8:+21175475 None:intergenic
GGTATATTATCTGTTGAAAA+TGG 0.316053 8:-21173273 MsG0880043246.01.T01:CDS
TTCAATTAATCATCTATCTA+TGG 0.338982 8:-21175795 MsG0880043246.01.T01:exon
ACGCAATTAATACATAAGTT+TGG 0.345471 8:+21173336 None:intergenic
ATGTATTAATTGCGTTTGTC+CGG 0.349424 8:-21173328 MsG0880043246.01.T01:CDS
GTTTGGAAGACTTGATATCT+TGG 0.353617 8:-21175434 MsG0880043246.01.T01:intron
TGGTTTCAACACAGACGATA+TGG 0.383538 8:-21174628 MsG0880043246.01.T01:intron
AATAAACAGCAATGAACAAA+TGG 0.385084 8:+21173090 None:intergenic
GAATTTGTTAAGACTCAGTT+TGG 0.409572 8:-21175451 MsG0880043246.01.T01:CDS
TGCATAATAACAAATTACTA+TGG 0.411597 8:-21174449 MsG0880043246.01.T01:CDS
GTAAATAATGCAGGAATCAA+TGG 0.443701 8:-21174648 MsG0880043246.01.T01:CDS
GTTAATGTTTCTTCTACTGC+AGG 0.448645 8:-21174365 MsG0880043246.01.T01:CDS
ATATGATAGGATATGTGTCT+TGG 0.480820 8:-21173131 MsG0880043246.01.T01:three_prime_UTR
TGTATTAATTGCGTTTGTCC+GGG 0.494685 8:-21173327 MsG0880043246.01.T01:CDS
CTATGGCAATTTCAAAAGAA+AGG 0.498850 8:-21175778 MsG0880043246.01.T01:intron
TACCTCGTCTATTAGCTCTT+CGG 0.500938 8:+21173488 None:intergenic
ATTAATTGAATCTAAGTGAA+TGG 0.503048 8:+21175808 None:intergenic
TTATTAATCAAATGAAATGT+GGG 0.504860 8:-21173072 MsG0880043246.01.T01:three_prime_UTR
TTCAGTACATATCAAACGAA+TGG 0.506130 8:-21173545 MsG0880043246.01.T01:intron
TTTATTAATCAAATGAAATG+TGG 0.512170 8:-21173073 MsG0880043246.01.T01:three_prime_UTR
CATGTGCAGGTAAATAATGC+AGG 0.516920 8:-21174657 MsG0880043246.01.T01:intron
ACATATCAAACGAATGGGCT+AGG 0.522049 8:-21173539 MsG0880043246.01.T01:CDS
GAATCAGATAACTGAAGAAG+AGG 0.526731 8:+21174398 None:intergenic
TCAGTACATATCAAACGAAT+GGG 0.528974 8:-21173544 MsG0880043246.01.T01:intron
GAAGAAACATTAACAATCAC+AGG 0.532332 8:+21174374 None:intergenic
TTATAGGCACAAAGATATGT+TGG 0.548863 8:+21173411 None:intergenic
ACAAGGTTCACTTGAAAACA+AGG 0.549206 8:-21173439 MsG0880043246.01.T01:CDS
ATAAACAGCAATGAACAAAT+GGG 0.555758 8:+21173091 None:intergenic
GCTAATAGACGAGGTACTGA+AGG 0.563423 8:-21173481 MsG0880043246.01.T01:CDS
TTTCAATGTATTAAAATGGG+GGG 0.578258 8:-21173154 MsG0880043246.01.T01:three_prime_UTR
ATTGAGTCAAACTTATGAGA+TGG 0.582165 8:-21174480 MsG0880043246.01.T01:CDS
GATAGGATATGTGTCTTGGA+TGG 0.587395 8:-21173127 MsG0880043246.01.T01:three_prime_UTR
AGCGAAGAAGTGTCCAGAAG+TGG 0.605903 8:+21173206 None:intergenic
GTTCACTTGAAAACAAGGGT+TGG 0.606906 8:-21173434 MsG0880043246.01.T01:CDS
TATTAATCAAATGAAATGTG+GGG 0.615096 8:-21173071 MsG0880043246.01.T01:three_prime_UTR
ATAAGCTAGAAGCCTTGTGT+AGG 0.622776 8:+21173365 None:intergenic
ATAATAACAAATTACTATGG+TGG 0.628363 8:-21174446 MsG0880043246.01.T01:CDS
CAGCTGTGAATTCCTACACA+AGG 0.629636 8:-21173377 MsG0880043246.01.T01:CDS
TTTCTGAAAGACTTCAAACA+AGG 0.631673 8:-21173456 MsG0880043246.01.T01:CDS
ACAGATATGAATAGAAACAC+AGG 0.645826 8:-21173294 MsG0880043246.01.T01:CDS
GAAGAAGTGTCCAGAAGTGG+TGG 0.653264 8:+21173209 None:intergenic
CAAGGTTCACTTGAAAACAA+GGG 0.660578 8:-21173438 MsG0880043246.01.T01:CDS
ATCCGAAGAGCTAATAGACG+AGG 0.706099 8:-21173490 MsG0880043246.01.T01:CDS
TGGTGGTAAAGAAACTACTG+AGG 0.726056 8:-21174429 MsG0880043246.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATTTTTTTAGTTATCT+TGG - Chr8:21173631-21173650 MsG0880043246.01.T01:intron 10.0%
!! CTTTGATAAAAGAAATTATA+AGG + Chr8:21173125-21173144 None:intergenic 15.0%
!! TTATTAATCAAATGAAATGT+GGG - Chr8:21175765-21175784 MsG0880043246.01.T01:intron 15.0%
!! TTTATTAATCAAATGAAATG+TGG - Chr8:21175764-21175783 MsG0880043246.01.T01:intron 15.0%
!!! AATTTCTACTAAATTTTAGT+TGG - Chr8:21175808-21175827 MsG0880043246.01.T01:five_prime_UTR 15.0%
!!! ATGTTTTTCATTAGTTAATT+TGG + Chr8:21175094-21175113 None:intergenic 15.0%
!!! TATAATTTCTTTTATCAAAG+TGG - Chr8:21173125-21173144 MsG0880043246.01.T01:three_prime_UTR 15.0%
!! AAAAAATGATGAACTCTATA+TGG + Chr8:21174761-21174780 None:intergenic 20.0%
!! AATTCTCTCCAATTAATAAT+TGG + Chr8:21174340-21174359 None:intergenic 20.0%
!! ATAATAACAAATTACTATGG+TGG - Chr8:21174391-21174410 MsG0880043246.01.T01:CDS 20.0%
!! ATTAATTGAATCTAAGTGAA+TGG + Chr8:21173032-21173051 None:intergenic 20.0%
!! ATTCTGAATTACCATTTATT+TGG - Chr8:21173543-21173562 MsG0880043246.01.T01:CDS 20.0%
!! CTAAGTTATTTATCAAAACA+AGG + Chr8:21173694-21173713 None:intergenic 20.0%
!! CTCAAAAAATTATCCTATAA+AGG - Chr8:21174673-21174692 MsG0880043246.01.T01:intron 20.0%
!! TATTAATCAAATGAAATGTG+GGG - Chr8:21175766-21175785 MsG0880043246.01.T01:intron 20.0%
!! TGAATTTCTCAAATTATTAG+TGG + Chr8:21174858-21174877 None:intergenic 20.0%
!! TGCATAATAACAAATTACTA+TGG - Chr8:21174388-21174407 MsG0880043246.01.T01:CDS 20.0%
!! TTCAATTAATCATCTATCTA+TGG - Chr8:21173042-21173061 MsG0880043246.01.T01:three_prime_UTR 20.0%
!! TTCTTGATTCTTAATTATCT+AGG - Chr8:21173604-21173623 MsG0880043246.01.T01:intron 20.0%
!!! AATTGGTACTTTTCATTTTT+CGG - Chr8:21174732-21174751 MsG0880043246.01.T01:intron 20.0%
!!! AGGTTTTCAATGTATTAAAA+TGG - Chr8:21175679-21175698 MsG0880043246.01.T01:intron 20.0%
!!! ATTATCACAAATTTTAGTCA+TGG + Chr8:21174123-21174142 None:intergenic 20.0%
!!! CAATATTTTTCGTTTTTTAG+AGG + Chr8:21174594-21174613 None:intergenic 20.0%
!!! GGTTTTCAATGTATTAAAAT+GGG - Chr8:21175680-21175699 MsG0880043246.01.T01:intron 20.0%
!!! GTTTTCAATGTATTAAAATG+GGG - Chr8:21175681-21175700 MsG0880043246.01.T01:intron 20.0%
!!! TTTCTTTGTTATGAATATTC+AGG - Chr8:21174309-21174328 MsG0880043246.01.T01:intron 20.0%
!!! TTTTCAATGTATTAAAATGG+GGG - Chr8:21175682-21175701 MsG0880043246.01.T01:intron 20.0%
! AATAAACAGCAATGAACAAA+TGG + Chr8:21175750-21175769 None:intergenic 25.0%
! ACAAACTTTGGATAAACTAA+TGG - Chr8:21174075-21174094 MsG0880043246.01.T01:intron 25.0%
! ACAGTTTATTTATGTTGTTC+AGG + Chr8:21175255-21175274 None:intergenic 25.0%
! ACGCAATTAATACATAAGTT+TGG + Chr8:21175504-21175523 None:intergenic 25.0%
! ACTAACATAAGACAGATATA+AGG + Chr8:21175028-21175047 None:intergenic 25.0%
! ACTAATGAAATACACATCAA+AGG - Chr8:21175067-21175086 MsG0880043246.01.T01:intron 25.0%
! ACTGTTTGAAAGAACTTATA+GGG + Chr8:21173890-21173909 None:intergenic 25.0%
! AGGTACCTTTAATGTTTATT+TGG - Chr8:21173079-21173098 MsG0880043246.01.T01:three_prime_UTR 25.0%
! ATAAACAGCAATGAACAAAT+GGG + Chr8:21175749-21175768 None:intergenic 25.0%
! ATCATAATGATCCAGAAAAT+TGG + Chr8:21173455-21173474 None:intergenic 25.0%
! ATTGGAAACTGTTAAAAGAT+TGG - Chr8:21173209-21173228 MsG0880043246.01.T01:CDS 25.0%
! GGTATATTATCTGTTGAAAA+TGG - Chr8:21175564-21175583 MsG0880043246.01.T01:CDS 25.0%
! TTCAGTGTAATTATATGTGT+TGG - Chr8:21174874-21174893 MsG0880043246.01.T01:intron 25.0%
! TTGATGTGTATTTCATTAGT+CGG + Chr8:21175067-21175086 None:intergenic 25.0%
! TTGGTCCAAATAAACATTAA+AGG + Chr8:21173087-21173106 None:intergenic 25.0%
! TTTCAATGTATTAAAATGGG+GGG - Chr8:21175683-21175702 MsG0880043246.01.T01:intron 25.0%
!! AAGGATCTTTTAGCATTTAT+TGG + Chr8:21173106-21173125 None:intergenic 25.0%
!! AAGTCTTTGAATTTTTCTGA+AGG + Chr8:21174564-21174583 None:intergenic 25.0%
!! ATCAAATAAAGGTATAGGAT+TGG - Chr8:21173191-21173210 MsG0880043246.01.T01:CDS 25.0%
!! TTAATGTAAAGCCAATTTTC+TGG - Chr8:21173441-21173460 MsG0880043246.01.T01:CDS 25.0%
!! TTGTTTTCTTTAAACTGTCT+GGG - Chr8:21174241-21174260 MsG0880043246.01.T01:intron 25.0%
!! TTTTATCCAGTGTTCAAAAA+TGG + Chr8:21173774-21173793 None:intergenic 25.0%
!!! AGAAAATTGGCTTTACATTA+AGG + Chr8:21173442-21173461 None:intergenic 25.0%
!!! ATATCTGTTTTAACAAAACC+CGG + Chr8:21175531-21175550 None:intergenic 25.0%
!!! ATTGGTACTTTTCATTTTTC+GGG - Chr8:21174733-21174752 MsG0880043246.01.T01:intron 25.0%
AAAAGTCCAGGCTATTATTA+TGG + Chr8:21174160-21174179 None:intergenic 30.0%
AATAGCCTTTGATTAAGCTT+TGG - Chr8:21174286-21174305 MsG0880043246.01.T01:intron 30.0%
ACAGATATGAATAGAAACAC+AGG - Chr8:21175543-21175562 MsG0880043246.01.T01:CDS 30.0%
ACATACAAGAAAAACATTCG+AGG - Chr8:21175131-21175150 MsG0880043246.01.T01:intron 30.0%
ACATTGTAACTGATGTAAAG+TGG + Chr8:21175231-21175250 None:intergenic 30.0%
ACCAATGAAGCAATACTAAA+AGG + Chr8:21173365-21173384 None:intergenic 30.0%
AGAAACCAAAGCTTAATCAA+AGG + Chr8:21174294-21174313 None:intergenic 30.0%
AGGTGGAACCAATTATTAAT+TGG - Chr8:21174329-21174348 MsG0880043246.01.T01:intron 30.0%
ATATGATAGGATATGTGTCT+TGG - Chr8:21175706-21175725 MsG0880043246.01.T01:intron 30.0%
ATGTATTAATTGCGTTTGTC+CGG - Chr8:21175509-21175528 MsG0880043246.01.T01:CDS 30.0%
ATTGAGTCAAACTTATGAGA+TGG - Chr8:21174357-21174376 MsG0880043246.01.T01:CDS 30.0%
CTATCAGAGATTATTTAGAC+TGG + Chr8:21174641-21174660 None:intergenic 30.0%
CTATGGCAATTTCAAAAGAA+AGG - Chr8:21173059-21173078 MsG0880043246.01.T01:three_prime_UTR 30.0%
CTGATGTAAAGTGGTAATTT+CGG + Chr8:21175222-21175241 None:intergenic 30.0%
GAAGAAACATTAACAATCAC+AGG + Chr8:21174466-21174485 None:intergenic 30.0%
GACTGTTTGAAAGAACTTAT+AGG + Chr8:21173891-21173910 None:intergenic 30.0%
GGTTAATTCAGAGTCTATAA+CGG - Chr8:21175170-21175189 MsG0880043246.01.T01:intron 30.0%
GTAAATAATGCAGGAATCAA+TGG - Chr8:21174189-21174208 MsG0880043246.01.T01:intron 30.0%
GTTGTTACAGGATCAAATAA+AGG - Chr8:21173180-21173199 MsG0880043246.01.T01:exon 30.0%
GTTTATCCAAAGTTTGTAAC+AGG + Chr8:21174072-21174091 None:intergenic 30.0%
TCAGTACATATCAAACGAAT+GGG - Chr8:21175293-21175312 MsG0880043246.01.T01:intron 30.0%
TCATAGGTGAAAACACATTT+CGG + Chr8:21174790-21174809 None:intergenic 30.0%
TCTCAAATTATTAGTGGTGT+TGG + Chr8:21174852-21174871 None:intergenic 30.0%
TGATAAAAGCTCTTAGCAAA+AGG + Chr8:21173723-21173742 None:intergenic 30.0%
TTATAGGCACAAAGATATGT+TGG + Chr8:21175429-21175448 None:intergenic 30.0%
TTCAGTACATATCAAACGAA+TGG - Chr8:21175292-21175311 MsG0880043246.01.T01:intron 30.0%
TTCTTCATTCTCATTCTCAT+AGG - Chr8:21173668-21173687 MsG0880043246.01.T01:intron 30.0%
! ACAGGATCAAATAAAGGTAT+AGG - Chr8:21173186-21173205 MsG0880043246.01.T01:CDS 30.0%
! CTTTGTTATGAATATTCAGG+TGG - Chr8:21174312-21174331 MsG0880043246.01.T01:intron 30.0%
! GTAATTATATGTGTTGGTGT+CGG - Chr8:21174880-21174899 MsG0880043246.01.T01:intron 30.0%
! TACCATTTATTTGGTGAATC+TGG - Chr8:21173552-21173571 MsG0880043246.01.T01:intron 30.0%
! TCCTTTTAGTATTGCTTCAT+TGG - Chr8:21173361-21173380 MsG0880043246.01.T01:CDS 30.0%
! TTTCTGAAAGACTTCAAACA+AGG - Chr8:21175381-21175400 MsG0880043246.01.T01:intron 30.0%
!! AGATTTGTCTTGCTTTTGAA+AGG - Chr8:21175659-21175678 MsG0880043246.01.T01:intron 30.0%
!! CGAGGATGTTTTTATAAGTT+TGG - Chr8:21175149-21175168 MsG0880043246.01.T01:intron 30.0%
!! CTTGTTTTCTTTAAACTGTC+TGG - Chr8:21174240-21174259 MsG0880043246.01.T01:intron 30.0%
!! GAATTTGTTAAGACTCAGTT+TGG - Chr8:21173386-21173405 MsG0880043246.01.T01:CDS 30.0%
!! GTTAAAAGATTGGCTTCAAA+TGG - Chr8:21173219-21173238 MsG0880043246.01.T01:CDS 30.0%
!! TTGGTACTTTTCATTTTTCG+GGG - Chr8:21174734-21174753 MsG0880043246.01.T01:intron 30.0%
!!! ACTTATTTTAGCTGATCTAC+TGG + Chr8:21173929-21173948 None:intergenic 30.0%
ACAAGGTTCACTTGAAAACA+AGG - Chr8:21175398-21175417 MsG0880043246.01.T01:intron 35.0%
AGCTAGATCAGAGAAAAAAG+AGG - Chr8:21173260-21173279 MsG0880043246.01.T01:CDS 35.0%
AGTTCACCATAATAATAGCC+TGG - Chr8:21174151-21174170 MsG0880043246.01.T01:intron 35.0%
ATCCAAAGTTTGTAACAGGT+TGG + Chr8:21174068-21174087 None:intergenic 35.0%
CAAGGTTCACTTGAAAACAA+GGG - Chr8:21175399-21175418 MsG0880043246.01.T01:intron 35.0%
CACCAGATTCACCAAATAAA+TGG + Chr8:21173557-21173576 None:intergenic 35.0%
CAGGCAAATTAGTCCTTTAT+AGG + Chr8:21174689-21174708 None:intergenic 35.0%
CCTCAAAAGTCTCAAATATC+AGG + Chr8:21174708-21174727 None:intergenic 35.0%
GAATCAGATAACTGAAGAAG+AGG + Chr8:21174442-21174461 None:intergenic 35.0%
GCACAAACACTTCAGATTAA+AGG - Chr8:21174022-21174041 MsG0880043246.01.T01:intron 35.0%
GCTAGATCAGAGAAAAAAGA+GGG - Chr8:21173261-21173280 MsG0880043246.01.T01:CDS 35.0%
GTTAATGTTTCTTCTACTGC+AGG - Chr8:21174472-21174491 MsG0880043246.01.T01:CDS 35.0%
GTTTGGAAGACTTGATATCT+TGG - Chr8:21173403-21173422 MsG0880043246.01.T01:CDS 35.0%
TCCAAAGTTTGTAACAGGTT+GGG + Chr8:21174067-21174086 None:intergenic 35.0%
TGTATTAATTGCGTTTGTCC+GGG - Chr8:21175510-21175529 MsG0880043246.01.T01:CDS 35.0%
TTAAAATGGGGGGATATGAT+AGG - Chr8:21175693-21175712 MsG0880043246.01.T01:intron 35.0%
TTCAGATATGCTGTTGTTAC+AGG - Chr8:21173168-21173187 MsG0880043246.01.T01:exon 35.0%
TTCTACTGCAGGACTATTAA+AGG - Chr8:21174483-21174502 MsG0880043246.01.T01:CDS 35.0%
! TAAACTGTCTGGGATGATAA+TGG - Chr8:21174251-21174270 MsG0880043246.01.T01:intron 35.0%
! TTATTTGGTGAATCTGGTGA+AGG - Chr8:21173558-21173577 MsG0880043246.01.T01:intron 35.0%
!! CCTGATATTTGAGACTTTTG+AGG - Chr8:21174705-21174724 MsG0880043246.01.T01:intron 35.0%
!! GAGACTTTTGAGGACTAAAT+TGG - Chr8:21174715-21174734 MsG0880043246.01.T01:intron 35.0%
!!! AGCTGCTTTTGAGAGTTTAT+AGG + Chr8:21175445-21175464 None:intergenic 35.0%
!!! CATAAGCCATTTTTGAACAC+TGG - Chr8:21173765-21173784 MsG0880043246.01.T01:intron 35.0%
CATGTGCAGGTAAATAATGC+AGG - Chr8:21174180-21174199 MsG0880043246.01.T01:intron 40.0%
CTGCAGGACTATTAAAGGTA+AGG - Chr8:21174488-21174507 MsG0880043246.01.T01:CDS 40.0%
GATAGGATATGTGTCTTGGA+TGG - Chr8:21175710-21175729 MsG0880043246.01.T01:intron 40.0%
GCACGAACACTTATCAGATT+AGG + Chr8:21174935-21174954 None:intergenic 40.0%
GTTCACTTGAAAACAAGGGT+TGG - Chr8:21175403-21175422 MsG0880043246.01.T01:intron 40.0%
TCACCTATGAAGCATGGATA+CGG - Chr8:21174800-21174819 MsG0880043246.01.T01:intron 40.0%
TCCCAACCTGTTACAAACTT+TGG - Chr8:21174063-21174082 MsG0880043246.01.T01:intron 40.0%
TGGTGGTAAAGAAACTACTG+AGG - Chr8:21174408-21174427 MsG0880043246.01.T01:CDS 40.0%
TGGTTTCAACACAGACGATA+TGG - Chr8:21174209-21174228 MsG0880043246.01.T01:intron 40.0%
! ACATATCAAACGAATGGGCT+AGG - Chr8:21175298-21175317 MsG0880043246.01.T01:intron 40.0%
! ATAAGCTAGAAGCCTTGTGT+AGG + Chr8:21175475-21175494 None:intergenic 40.0%
! CTTCAGATTAAAGGCGTGTT+TGG - Chr8:21174031-21174050 MsG0880043246.01.T01:intron 40.0%
! GTGTTTTCACCTATGAAGCA+TGG - Chr8:21174794-21174813 MsG0880043246.01.T01:intron 40.0%
! TACCTCGTCTATTAGCTCTT+CGG + Chr8:21175352-21175371 None:intergenic 40.0%
ATCCGAAGAGCTAATAGACG+AGG - Chr8:21175347-21175366 MsG0880043246.01.T01:intron 45.0%
ATGAAGCATGGATACGGACA+CGG - Chr8:21174806-21174825 MsG0880043246.01.T01:intron 45.0%
CAGCTGTGAATTCCTACACA+AGG - Chr8:21175460-21175479 MsG0880043246.01.T01:CDS 45.0%
TACCTGCACATGAAAAGTCC+AGG + Chr8:21174172-21174191 None:intergenic 45.0%
TGTCCGTATCCATGCTTCAT+AGG + Chr8:21174806-21174825 None:intergenic 45.0%
TTATCAGATTAGGCGTGTCC+AGG + Chr8:21174925-21174944 None:intergenic 45.0%
!! GCTAATAGACGAGGTACTGA+AGG - Chr8:21175356-21175375 MsG0880043246.01.T01:intron 45.0%
TCGAGTGATTCCACCACTTC+TGG - Chr8:21175618-21175637 MsG0880043246.01.T01:intron 50.0%
! AGCCTGGACTTTTCATGTGC+AGG - Chr8:21174167-21174186 MsG0880043246.01.T01:intron 50.0%
!! AGCGAAGAAGTGTCCAGAAG+TGG + Chr8:21175634-21175653 None:intergenic 50.0%
!! GAAGAAGTGTCCAGAAGTGG+TGG + Chr8:21175631-21175650 None:intergenic 50.0%
GATTAGGCGTGTCCAGGTGT+CGG + Chr8:21174919-21174938 None:intergenic 55.0%
TGTTGGCGTCTCAGTGTGTC+TGG + Chr8:21174835-21174854 None:intergenic 55.0%
CACGACACGTGTCCGACACC+TGG - Chr8:21174904-21174923 MsG0880043246.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 21173025 21175834 21173025 ID=MsG0880043246.01;Name=MsG0880043246.01
Chr8 mRNA 21173025 21175834 21173025 ID=MsG0880043246.01.T01;Parent=MsG0880043246.01;Name=MsG0880043246.01.T01;_AED=0.48;_eAED=0.48;_QI=36|0.75|0.8|1|0.75|0.6|5|157|256
Chr8 exon 21173025 21173562 21173025 ID=MsG0880043246.01.T01:exon:5657;Parent=MsG0880043246.01.T01
Chr8 exon 21174355 21174528 21174355 ID=MsG0880043246.01.T01:exon:5656;Parent=MsG0880043246.01.T01
Chr8 exon 21174629 21174670 21174629 ID=MsG0880043246.01.T01:exon:5655;Parent=MsG0880043246.01.T01
Chr8 exon 21175435 21175588 21175435 ID=MsG0880043246.01.T01:exon:5654;Parent=MsG0880043246.01.T01
Chr8 exon 21175779 21175834 21175779 ID=MsG0880043246.01.T01:exon:5653;Parent=MsG0880043246.01.T01
Chr8 five_prime_UTR 21175799 21175834 21175799 ID=MsG0880043246.01.T01:five_prime_utr;Parent=MsG0880043246.01.T01
Chr8 CDS 21175779 21175798 21175779 ID=MsG0880043246.01.T01:cds;Parent=MsG0880043246.01.T01
Chr8 CDS 21175435 21175588 21175435 ID=MsG0880043246.01.T01:cds;Parent=MsG0880043246.01.T01
Chr8 CDS 21174629 21174670 21174629 ID=MsG0880043246.01.T01:cds;Parent=MsG0880043246.01.T01
Chr8 CDS 21174355 21174528 21174355 ID=MsG0880043246.01.T01:cds;Parent=MsG0880043246.01.T01
Chr8 CDS 21173182 21173562 21173182 ID=MsG0880043246.01.T01:cds;Parent=MsG0880043246.01.T01
Chr8 three_prime_UTR 21173025 21173181 21173025 ID=MsG0880043246.01.T01:three_prime_utr;Parent=MsG0880043246.01.T01
Gene Sequence

>MsG0880043246.01.T01

ATGGCAATTTCAAAAGAAAGAGAAAAAAGAGGGATTCAAGCTTTTGAGAAATTGAAGAAAGAATTTGAATTTTGTAATCTTGTTGTTTTTCATCAACTTGATGTTACTGATCCTTTTAGTATTGCTTCATTGGTTGAATTTGTTAAGACTCAGTTTGGAAGACTTGATATCTTGGTAAATAATGCAGGAATCAATGGTTTCAACACAGACGATATGGTGGAACCAATTATTAATTGGAGAGAATTGAGTCAAACTTATGAGATGGCAGAAAATTGCATAATAACAAATTACTATGGTGGTAAAGAAACTACTGAGGCTTTTCTTCCTCTTCTTCAGTTATCTGATTCACCTGTGATTGTTAATGTTTCTTCTACTGCAGGACTATTAAAGTACATATCAAACGAATGGGCTAGGAGTGTGTTAGACGATACTGAAAATCTATCCGAAGAGCTAATAGACGAGGTACTGAAGGAGTTTCTGAAAGACTTCAAACAAGGTTCACTTGAAAACAAGGGTTGGCCAACATATCTTTGTGCCTATAAACTCTCAAAAGCAGCTGTGAATTCCTACACAAGGCTTCTAGCTTATAGACATCCAAACTTATGTATTAATTGCGTTTGTCCGGGTTTTGTTAAAACAGATATGAATAGAAACACAGGTATATTATCTGTTGAAAATGGTGCTGCTAGTGTTGTCAGATTAGCATTACTTTCGAGTGATTCCACCACTTCTGGACACTTCTTCGCTCGTCAAGATTTGTCTTGCTTTTGA
Protein sequence

>MsG0880043246.01.T01

MAISKEREKRGIQAFEKLKKEFEFCNLVVFHQLDVTDPFSIASLVEFVKTQFGRLDILVNNAGINGFNTDDMVEPIINWRELSQTYEMAENCIITNYYGGKETTEAFLPLLQLSDSPVIVNVSSTAGLLKYISNEWARSVLDDTENLSEELIDEVLKEFLKDFKQGSLENKGWPTYLCAYKLSKAAVNSYTRLLAYRHPNLCINCVCPGFVKTDMNRNTGILSVENGAASVVRLALLSSDSTTSGHFFARQDLSCF*