Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024719.01.T01 | XP_003611549.2 | 91.176 | 102 | 4 | 2 | 1 | 97 | 1 | 102 | 3.06E-56 | 180 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048347.01 | MsG0580024719.01 | 0.806587 | 7.653267e-50 | 4.169690e-47 |
| MsG0080048352.01 | MsG0580024719.01 | 0.809784 | 1.594159e-50 | 9.446663e-48 |
| MsG0180001691.01 | MsG0580024719.01 | 0.803000 | 4.296190e-49 | 2.134687e-46 |
| MsG0180003830.01 | MsG0580024719.01 | 0.823598 | 1.270777e-53 | 1.099156e-50 |
| MsG0180003993.01 | MsG0580024719.01 | 0.815079 | 1.110775e-51 | 7.583166e-49 |
| MsG0180005356.01 | MsG0580024719.01 | 0.836314 | 1.006127e-56 | 1.263441e-53 |
| MsG0180005358.01 | MsG0580024719.01 | 0.823954 | 1.048513e-53 | 9.161082e-51 |
| MsG0180005648.01 | MsG0580024719.01 | 0.810953 | 8.920658e-51 | 5.452204e-48 |
| MsG0280011238.01 | MsG0580024719.01 | 0.809696 | 1.665897e-50 | 9.848314e-48 |
| MsG0280011267.01 | MsG0580024719.01 | 0.805597 | 1.236261e-49 | 6.567146e-47 |
| MsG0380013300.01 | MsG0580024719.01 | 0.800890 | 1.165609e-48 | 5.490246e-46 |
| MsG0480019532.01 | MsG0580024719.01 | 0.832596 | 8.639336e-56 | 9.701755e-53 |
| MsG0480022310.01 | MsG0580024719.01 | 0.833735 | 4.495167e-56 | 5.222871e-53 |
| MsG0480022736.01 | MsG0580024719.01 | 0.800947 | 1.134718e-48 | 5.352461e-46 |
| MsG0580024218.01 | MsG0580024719.01 | 0.841708 | 4.034279e-58 | 5.977298e-55 |
| MsG0580024219.01 | MsG0580024719.01 | 0.807194 | 5.693257e-50 | 3.151023e-47 |
| MsG0580024719.01 | MsG0580025682.01 | 0.823492 | 1.345734e-53 | 1.160511e-50 |
| MsG0580024719.01 | MsG0580030133.01 | 0.829385 | 5.301832e-55 | 5.418344e-52 |
| MsG0580024719.01 | MsG0780036661.01 | 0.806836 | 6.778906e-50 | 3.717271e-47 |
| MsG0580024719.01 | MsG0780040613.01 | 0.822019 | 2.963495e-53 | 2.450791e-50 |
| MsG0580024719.01 | MsG0780041316.01 | 0.827106 | 1.878684e-54 | 1.796124e-51 |
| MsG0580024719.01 | MsG0780041556.01 | 0.800816 | 1.206832e-48 | 5.673320e-46 |
| MsG0280009232.01 | MsG0580024719.01 | 0.800590 | 1.342032e-48 | 6.272979e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024719.01.T01 | MTR_5g015190 | 91.176 | 102 | 4 | 2 | 1 | 97 | 1 | 102 | 3.70e-60 | 180 |
| MsG0580024719.01.T01 | MTR_4g097350 | 54.839 | 62 | 27 | 1 | 9 | 69 | 32 | 93 | 1.98e-14 | 66.2 |
| MsG0580024719.01.T01 | MTR_4g097280 | 54.839 | 62 | 27 | 1 | 9 | 69 | 31 | 92 | 2.79e-13 | 63.2 |
| MsG0580024719.01.T01 | MTR_4g097360 | 56.364 | 55 | 23 | 1 | 16 | 69 | 35 | 89 | 1.98e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024719.01.T01 | AT2G22880 | 39.091 | 110 | 60 | 4 | 1 | 110 | 1 | 103 | 1.60e-12 | 59.3 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACCCTAACTGCTGTTAA+AGG | 0.195754 | 5:+8767592 | None:intergenic |
| CAACAATTTGTTAGTGATAA+AGG | 0.212595 | 5:-8767493 | MsG0580024719.01.T01:CDS |
| GACAAAGCAATCAGGTTTAT+TGG | 0.268685 | 5:-8767443 | MsG0580024719.01.T01:CDS |
| TGTTGTTGAGGCTTGAATTC+TGG | 0.279950 | 5:+8767517 | None:intergenic |
| AACAATTTGTTAGTGATAAA+GGG | 0.288388 | 5:-8767492 | MsG0580024719.01.T01:CDS |
| CACAACCTTTAACAGCAGTT+AGG | 0.395073 | 5:-8767597 | MsG0580024719.01.T01:CDS |
| CTTCACCATCTTCTCAGAGT+TGG | 0.407398 | 5:-8767408 | MsG0580024719.01.T01:CDS |
| CACCATCTTCTCAGAGTTGG+TGG | 0.431356 | 5:-8767405 | MsG0580024719.01.T01:CDS |
| ATTATGAGACTTAGGATAAG+AGG | 0.439722 | 5:+8767684 | None:intergenic |
| AATGAATTATTATGAGACTT+AGG | 0.448016 | 5:+8767676 | None:intergenic |
| ACAACCTTTAACAGCAGTTA+GGG | 0.458936 | 5:-8767596 | MsG0580024719.01.T01:CDS |
| AGTGGTGTGACAAAGCAATC+AGG | 0.507552 | 5:-8767451 | MsG0580024719.01.T01:CDS |
| CTAACTGCTGTTAAAGGTTG+TGG | 0.514313 | 5:+8767598 | None:intergenic |
| GACCACCAACTCTGAGAAGA+TGG | 0.520594 | 5:+8767403 | None:intergenic |
| ATTGTGGCTTTCTAACGGAA+TGG | 0.529161 | 5:+8767650 | None:intergenic |
| AGACTTAGGATAAGAGGTGG+TGG | 0.557065 | 5:+8767690 | None:intergenic |
| GGTGGTGGAATAAGCTTCCA+TGG | 0.561828 | 5:+8767705 | None:intergenic |
| ACAATTTGTTAGTGATAAAG+GGG | 0.570288 | 5:-8767491 | MsG0580024719.01.T01:CDS |
| ACAAATTGTTGATGTTGTTG+AGG | 0.598417 | 5:+8767505 | None:intergenic |
| GAAAGCCACAATCAAAACCA+TGG | 0.621885 | 5:-8767639 | MsG0580024719.01.T01:CDS |
| GAGTTAGTTCAACAACTCAC+AGG | 0.631511 | 5:-8767544 | MsG0580024719.01.T01:CDS |
| GTGCAATATCAAGAGAGTAG+TGG | 0.659499 | 5:-8767469 | MsG0580024719.01.T01:CDS |
| ATGAGACTTAGGATAAGAGG+TGG | 0.667730 | 5:+8767687 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAATTATTATGAGACTT+AGG | + | Chr5:8767420-8767439 | None:intergenic | 20.0% |
| !!! | AACAATTTGTTAGTGATAAA+GGG | - | Chr5:8767601-8767620 | MsG0580024719.01.T01:CDS | 20.0% |
| !!! | ACAATTTGTTAGTGATAAAG+GGG | - | Chr5:8767602-8767621 | MsG0580024719.01.T01:CDS | 25.0% |
| !!! | CAACAATTTGTTAGTGATAA+AGG | - | Chr5:8767600-8767619 | MsG0580024719.01.T01:CDS | 25.0% |
| ACAAATTGTTGATGTTGTTG+AGG | + | Chr5:8767591-8767610 | None:intergenic | 30.0% | |
| ATTATGAGACTTAGGATAAG+AGG | + | Chr5:8767412-8767431 | None:intergenic | 30.0% | |
| !! | TTTTGATTGTGGCTTTCTAA+CGG | + | Chr5:8767451-8767470 | None:intergenic | 30.0% |
| !!! | TTTTTCCATGGTTTTGATTG+TGG | + | Chr5:8767462-8767481 | None:intergenic | 30.0% |
| ACAACCTTTAACAGCAGTTA+GGG | - | Chr5:8767497-8767516 | MsG0580024719.01.T01:CDS | 35.0% | |
| GACAAAGCAATCAGGTTTAT+TGG | - | Chr5:8767650-8767669 | MsG0580024719.01.T01:CDS | 35.0% | |
| TAAACCCTAACTGCTGTTAA+AGG | + | Chr5:8767504-8767523 | None:intergenic | 35.0% | |
| ACCATGGAAAAAAGCTTCAG+TGG | - | Chr5:8767470-8767489 | MsG0580024719.01.T01:CDS | 40.0% | |
| ATGAGACTTAGGATAAGAGG+TGG | + | Chr5:8767409-8767428 | None:intergenic | 40.0% | |
| ATTGTGGCTTTCTAACGGAA+TGG | + | Chr5:8767446-8767465 | None:intergenic | 40.0% | |
| CACAACCTTTAACAGCAGTT+AGG | - | Chr5:8767496-8767515 | MsG0580024719.01.T01:CDS | 40.0% | |
| CTAACTGCTGTTAAAGGTTG+TGG | + | Chr5:8767498-8767517 | None:intergenic | 40.0% | |
| GAAAGCCACAATCAAAACCA+TGG | - | Chr5:8767454-8767473 | MsG0580024719.01.T01:CDS | 40.0% | |
| GAGTTAGTTCAACAACTCAC+AGG | - | Chr5:8767549-8767568 | MsG0580024719.01.T01:CDS | 40.0% | |
| GTGCAATATCAAGAGAGTAG+TGG | - | Chr5:8767624-8767643 | MsG0580024719.01.T01:CDS | 40.0% | |
| TGTTGTTGAGGCTTGAATTC+TGG | + | Chr5:8767579-8767598 | None:intergenic | 40.0% | |
| AGACTTAGGATAAGAGGTGG+TGG | + | Chr5:8767406-8767425 | None:intergenic | 45.0% | |
| AGTGGTGTGACAAAGCAATC+AGG | - | Chr5:8767642-8767661 | MsG0580024719.01.T01:CDS | 45.0% | |
| CTTCACCATCTTCTCAGAGT+TGG | - | Chr5:8767685-8767704 | MsG0580024719.01.T01:CDS | 45.0% | |
| !!! | GCCACTGAAGCTTTTTTCCA+TGG | + | Chr5:8767474-8767493 | None:intergenic | 45.0% |
| CACCATCTTCTCAGAGTTGG+TGG | - | Chr5:8767688-8767707 | MsG0580024719.01.T01:CDS | 50.0% | |
| ! | GACCACCAACTCTGAGAAGA+TGG | + | Chr5:8767693-8767712 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 8767390 | 8767725 | 8767390 | ID=MsG0580024719.01;Name=MsG0580024719.01 |
| Chr5 | mRNA | 8767390 | 8767725 | 8767390 | ID=MsG0580024719.01.T01;Parent=MsG0580024719.01;Name=MsG0580024719.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|111 |
| Chr5 | exon | 8767390 | 8767725 | 8767390 | ID=MsG0580024719.01.T01:exon:26397;Parent=MsG0580024719.01.T01 |
| Chr5 | CDS | 8767390 | 8767725 | 8767390 | ID=MsG0580024719.01.T01:cds;Parent=MsG0580024719.01.T01 |
| Gene Sequence |
| Protein sequence |
