AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022240.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022240.01.T01 MTR_4g082900 89.474 57 5 1 1 56 1 57 4.10e-21 78.6
MsG0480022240.01.T01 MTR_4g082883 68.519 54 14 2 8 58 26 79 1.17e-14 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTGGACCTAACGATATTT+TGG 0.245207 4:-70038499 MsG0480022240.01.T01:CDS
AAAACCAAAGGTGCGGTTAA+TGG 0.283737 4:-70038317 None:intergenic
AGCACCATTAACCGCACCTT+TGG 0.426753 4:+70038313 None:intergenic
GCACTTGCCAAAATATCGTT+AGG 0.455143 4:+70038492 None:intergenic
TAAAACCAAACCAGATCCTA+TGG 0.485210 4:-70038341 MsG0480022240.01.T01:CDS
TAAACAACAAAATCGATGGC+TGG 0.548436 4:-70038516 None:intergenic
GCAGGTGTTCATAAGACTGT+AGG 0.564597 4:-70038393 MsG0480022240.01.T01:CDS
ACTGTAGGAAATGCTGCAGC+TGG 0.565617 4:-70038441 MsG0480022240.01.T01:CDS
GCTTCAGCACAGAAAACTGT+AGG 0.594928 4:-70038456 MsG0480022240.01.T01:CDS
AAAACTGTGGGAAATGCTGC+AGG 0.601750 4:-70038411 MsG0480022240.01.T01:CDS
AGATCCTATGGCAAAACCAA+AGG 0.621329 4:-70038329 MsG0480022240.01.T01:CDS
AGCTGGTGCCCAGAAAACTG+TGG 0.654418 4:-70038424 MsG0480022240.01.T01:CDS
TGTAGGAGACTATGCTACCA+TGG 0.655972 4:-70038376 MsG0480022240.01.T01:CDS
CTATGGCAAAACCAAAGGTG+CGG 0.678266 4:-70038324 MsG0480022240.01.T01:CDS
GCTGGTGCCCAGAAAACTGT+GGG 0.683027 4:-70038423 MsG0480022240.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAAAACCAAACCAGATCCTA+TGG - Chr4:70038480-70038499 MsG0480022240.01.T01:CDS 35.0%
! TTTTACATAATCAGCAGCCA+TGG + Chr4:70038465-70038484 None:intergenic 35.0%
AGATCCTATGGCAAAACCAA+AGG - Chr4:70038492-70038511 MsG0480022240.01.T01:CDS 40.0%
GCACTTGCCAAAATATCGTT+AGG + Chr4:70038332-70038351 None:intergenic 40.0%
! AGCATTTCCCACAGTTTTCT+GGG + Chr4:70038408-70038427 None:intergenic 40.0%
! TTTTGCCATAGGATCTGGTT+TGG + Chr4:70038488-70038507 None:intergenic 40.0%
!!! CGATATTTTGGCAAGTGCAA+AGG - Chr4:70038334-70038353 MsG0480022240.01.T01:CDS 40.0%
!!! TTTGGTTTTGCCATAGGATC+TGG + Chr4:70038493-70038512 None:intergenic 40.0%
AAAACTGTGGGAAATGCTGC+AGG - Chr4:70038410-70038429 MsG0480022240.01.T01:CDS 45.0%
CTATGGCAAAACCAAAGGTG+CGG - Chr4:70038497-70038516 MsG0480022240.01.T01:CDS 45.0%
GCAGGTGTTCATAAGACTGT+AGG - Chr4:70038428-70038447 MsG0480022240.01.T01:CDS 45.0%
GCTTCAGCACAGAAAACTGT+AGG - Chr4:70038365-70038384 MsG0480022240.01.T01:CDS 45.0%
TGTAGGAGACTATGCTACCA+TGG - Chr4:70038445-70038464 MsG0480022240.01.T01:CDS 45.0%
! CAGCATTTCCCACAGTTTTC+TGG + Chr4:70038409-70038428 None:intergenic 45.0%
! GGCTGGACCTAACGATATTT+TGG - Chr4:70038322-70038341 MsG0480022240.01.T01:CDS 45.0%
ACTGTAGGAAATGCTGCAGC+TGG - Chr4:70038380-70038399 MsG0480022240.01.T01:CDS 50.0%
!!! CGCACCTTTGGTTTTGCCAT+AGG + Chr4:70038499-70038518 None:intergenic 50.0%
AGCTGGTGCCCAGAAAACTG+TGG - Chr4:70038397-70038416 MsG0480022240.01.T01:CDS 55.0%
GCTGGTGCCCAGAAAACTGT+GGG - Chr4:70038398-70038417 MsG0480022240.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70038320 70038523 70038320 ID=MsG0480022240.01;Name=MsG0480022240.01
Chr4 mRNA 70038320 70038523 70038320 ID=MsG0480022240.01.T01;Parent=MsG0480022240.01;Name=MsG0480022240.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|67
Chr4 exon 70038320 70038523 70038320 ID=MsG0480022240.01.T01:exon:11098;Parent=MsG0480022240.01.T01
Chr4 CDS 70038320 70038523 70038320 ID=MsG0480022240.01.T01:cds;Parent=MsG0480022240.01.T01
Gene Sequence

>MsG0480022240.01.T01

ATGGCTGGACCTAACGATATTTTGGCAAGTGCAAAGGATGCAGCAGCTTCAGCACAGAAAACTGTAGGAAATGCTGCAGCTGGTGCCCAGAAAACTGTGGGAAATGCTGCAGGTGTTCATAAGACTGTAGGAGACTATGCTACCATGGCTGCTGATTATGTAAAACCAAACCAGATCCTATGGCAAAACCAAAGGTGCGGTTAA

Protein sequence

>MsG0480022240.01.T01

MAGPNDILASAKDAAASAQKTVGNAAAGAQKTVGNAAGVHKTVGDYATMAADYVKPNQILWQNQRCG*