Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030559.01.T01 | XP_013451087.1 | 93.952 | 463 | 26 | 1 | 1 | 461 | 1 | 463 | 0 | 888 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030559.01.T01 | Q9ZWR8 | 35.43 | 477 | 268 | 16 | 9 | 461 | 8 | 468 | 2.11E-85 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030559.01.T01 | A0A072U623 | 93.952 | 463 | 26 | 1 | 1 | 461 | 1 | 463 | 0.0 | 888 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048036.01 | MsG0680030559.01 | 0.800382 | 1.479651e-48 | 6.880032e-46 |
| MsG0080048967.01 | MsG0680030559.01 | 0.804274 | 2.337311e-49 | 1.200030e-46 |
| MsG0180003240.01 | MsG0680030559.01 | 0.805352 | 1.391663e-49 | 7.346240e-47 |
| MsG0180005358.01 | MsG0680030559.01 | 0.806311 | 8.749578e-50 | 4.733638e-47 |
| MsG0180005996.01 | MsG0680030559.01 | 0.820265 | 7.516910e-53 | 5.919203e-50 |
| MsG0380011613.01 | MsG0680030559.01 | 0.821847 | 3.246898e-53 | 2.672371e-50 |
| MsG0380012008.01 | MsG0680030559.01 | 0.813529 | 2.443711e-51 | 1.599878e-48 |
| MsG0380013300.01 | MsG0680030559.01 | 0.833576 | 4.925152e-56 | 5.695278e-53 |
| MsG0380013449.01 | MsG0680030559.01 | 0.813311 | 2.729466e-51 | 1.776315e-48 |
| MsG0380013452.01 | MsG0680030559.01 | 0.822984 | 1.767986e-53 | 1.502967e-50 |
| MsG0380013458.01 | MsG0680030559.01 | 0.809045 | 2.297252e-50 | 1.334706e-47 |
| MsG0480022125.01 | MsG0680030559.01 | 0.843113 | 1.710753e-58 | 2.649115e-55 |
| MsG0480022735.01 | MsG0680030559.01 | 0.801340 | 9.429218e-49 | 4.491975e-46 |
| MsG0480022736.01 | MsG0680030559.01 | 0.803726 | 3.037895e-49 | 1.537945e-46 |
| MsG0480023107.01 | MsG0680030559.01 | 0.804024 | 2.634929e-49 | 1.344227e-46 |
| MsG0480023819.01 | MsG0680030559.01 | 0.809576 | 1.767661e-50 | 1.041700e-47 |
| MsG0580024218.01 | MsG0680030559.01 | 0.817475 | 3.234541e-52 | 2.358095e-49 |
| MsG0580025683.01 | MsG0680030559.01 | 0.844569 | 6.971007e-59 | 1.130150e-55 |
| MsG0380017690.01 | MsG0680030559.01 | 0.828124 | 1.070249e-54 | 1.054128e-51 |
| MsG0380018026.01 | MsG0680030559.01 | 0.819781 | 9.696522e-53 | 7.533947e-50 |
| MsG0580026925.01 | MsG0680030559.01 | 0.817843 | 2.672704e-52 | 1.968154e-49 |
| MsG0580030133.01 | MsG0680030559.01 | 0.827160 | 1.823539e-54 | 1.746232e-51 |
| MsG0580030145.01 | MsG0680030559.01 | 0.814981 | 1.168162e-51 | 7.953764e-49 |
| MsG0680030559.01 | MsG0680030996.01 | 0.845187 | 4.748929e-59 | 7.850149e-56 |
| MsG0680030559.01 | MsG0680031429.01 | 0.831888 | 1.293292e-55 | 1.422501e-52 |
| MsG0680030559.01 | MsG0780039190.01 | 0.805788 | 1.127245e-49 | 6.017724e-47 |
| MsG0680030559.01 | MsG0780039576.01 | 0.832641 | 8.419168e-56 | 9.467429e-53 |
| MsG0680030559.01 | MsG0780039968.01 | 0.820235 | 7.636331e-53 | 6.008133e-50 |
| MsG0680030559.01 | MsG0880043828.01 | 0.826657 | 2.404901e-54 | 2.269525e-51 |
| MsG0680030559.01 | MsG0880045677.01 | 0.812326 | 4.484819e-51 | 2.843068e-48 |
| MsG0280008405.01 | MsG0680030559.01 | 0.810956 | 8.907341e-51 | 5.444467e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030559.01.T01 | MTR_6g015815 | 93.952 | 463 | 26 | 1 | 1 | 461 | 1 | 463 | 0.0 | 888 |
| MsG0680030559.01.T01 | MTR_6g015850 | 80.043 | 461 | 91 | 1 | 1 | 461 | 1 | 460 | 0.0 | 763 |
| MsG0680030559.01.T01 | MTR_6g015830 | 76.940 | 464 | 103 | 3 | 1 | 461 | 1 | 463 | 0.0 | 729 |
| MsG0680030559.01.T01 | MTR_6g015315 | 57.511 | 466 | 182 | 9 | 1 | 461 | 1 | 455 | 5.26e-178 | 506 |
| MsG0680030559.01.T01 | MTR_6g015320 | 57.516 | 459 | 179 | 8 | 9 | 461 | 9 | 457 | 7.18e-177 | 503 |
| MsG0680030559.01.T01 | MTR_7g014230 | 46.781 | 466 | 228 | 10 | 8 | 461 | 4 | 461 | 2.85e-143 | 418 |
| MsG0680030559.01.T01 | MTR_7g014330 | 45.474 | 464 | 235 | 9 | 8 | 460 | 2 | 458 | 5.26e-141 | 412 |
| MsG0680030559.01.T01 | MTR_7g014250 | 45.494 | 466 | 232 | 10 | 8 | 460 | 2 | 458 | 1.55e-139 | 408 |
| MsG0680030559.01.T01 | MTR_7g014200 | 44.176 | 455 | 226 | 10 | 25 | 458 | 21 | 468 | 1.31e-114 | 345 |
| MsG0680030559.01.T01 | MTR_7g014160 | 42.251 | 471 | 231 | 13 | 20 | 458 | 12 | 473 | 4.27e-111 | 336 |
| MsG0680030559.01.T01 | MTR_6g015310 | 50.704 | 355 | 127 | 8 | 84 | 436 | 25 | 333 | 5.77e-108 | 323 |
| MsG0680030559.01.T01 | MTR_7g014610 | 40.870 | 460 | 247 | 9 | 22 | 458 | 18 | 475 | 2.20e-103 | 317 |
| MsG0680030559.01.T01 | MTR_7g013850 | 41.009 | 456 | 232 | 12 | 35 | 458 | 30 | 480 | 1.09e-100 | 310 |
| MsG0680030559.01.T01 | MTR_7g014360 | 41.237 | 388 | 200 | 9 | 80 | 460 | 22 | 388 | 5.71e-95 | 292 |
| MsG0680030559.01.T01 | MTR_7g014620 | 38.964 | 444 | 246 | 7 | 24 | 458 | 5 | 432 | 4.74e-89 | 278 |
| MsG0680030559.01.T01 | MTR_2g089765 | 35.685 | 482 | 268 | 15 | 9 | 461 | 17 | 485 | 3.69e-83 | 265 |
| MsG0680030559.01.T01 | MTR_2g089670 | 36.384 | 448 | 261 | 8 | 30 | 461 | 41 | 480 | 1.57e-82 | 263 |
| MsG0680030559.01.T01 | MTR_3g028210 | 34.177 | 474 | 276 | 12 | 9 | 461 | 9 | 467 | 7.28e-77 | 248 |
| MsG0680030559.01.T01 | MTR_8g012750 | 34.647 | 482 | 276 | 11 | 9 | 461 | 4 | 475 | 3.54e-75 | 243 |
| MsG0680030559.01.T01 | MTR_2g089735 | 35.056 | 445 | 240 | 14 | 47 | 461 | 39 | 464 | 4.14e-74 | 240 |
| MsG0680030559.01.T01 | MTR_3g087640 | 34.591 | 477 | 251 | 13 | 22 | 459 | 19 | 473 | 2.68e-72 | 236 |
| MsG0680030559.01.T01 | MTR_2g089650 | 34.183 | 471 | 252 | 15 | 25 | 461 | 20 | 466 | 4.11e-72 | 235 |
| MsG0680030559.01.T01 | MTR_7g015860 | 32.983 | 476 | 272 | 11 | 5 | 454 | 2 | 456 | 4.44e-69 | 227 |
| MsG0680030559.01.T01 | MTR_2g089755 | 34.120 | 466 | 245 | 16 | 34 | 461 | 37 | 478 | 1.19e-68 | 226 |
| MsG0680030559.01.T01 | MTR_7g014310 | 37.117 | 326 | 151 | 8 | 117 | 439 | 30 | 304 | 9.52e-57 | 190 |
| MsG0680030559.01.T01 | MTR_7g014630 | 40.702 | 285 | 149 | 9 | 9 | 281 | 10 | 286 | 6.31e-55 | 187 |
| MsG0680030559.01.T01 | MTR_2g089665 | 30.804 | 448 | 190 | 14 | 9 | 443 | 3 | 343 | 2.06e-47 | 167 |
| MsG0680030559.01.T01 | MTR_7g014180 | 53.548 | 155 | 68 | 2 | 25 | 175 | 19 | 173 | 9.40e-46 | 157 |
| MsG0680030559.01.T01 | MTR_7g014670 | 41.758 | 182 | 102 | 2 | 282 | 459 | 79 | 260 | 1.69e-39 | 143 |
| MsG0680030559.01.T01 | MTR_3g435300 | 40.580 | 207 | 102 | 4 | 25 | 218 | 15 | 213 | 8.91e-39 | 142 |
| MsG0680030559.01.T01 | MTR_8g075610 | 26.265 | 415 | 267 | 18 | 50 | 453 | 30 | 416 | 1.23e-26 | 111 |
| MsG0680030559.01.T01 | MTR_7g014205 | 42.748 | 131 | 61 | 3 | 298 | 426 | 2 | 120 | 5.53e-24 | 97.1 |
| MsG0680030559.01.T01 | MTR_7g014210 | 45.690 | 116 | 59 | 3 | 316 | 428 | 24 | 138 | 7.02e-24 | 98.2 |
| MsG0680030559.01.T01 | MTR_2g064495 | 25.481 | 416 | 274 | 13 | 50 | 454 | 39 | 429 | 1.04e-23 | 103 |
| MsG0680030559.01.T01 | MTR_2g105330 | 24.940 | 417 | 275 | 14 | 50 | 454 | 39 | 429 | 1.39e-22 | 100 |
| MsG0680030559.01.T01 | MTR_4g007540 | 25.243 | 412 | 273 | 13 | 50 | 450 | 39 | 426 | 2.42e-22 | 99.4 |
| MsG0680030559.01.T01 | MTR_7g014340 | 59.155 | 71 | 27 | 1 | 8 | 78 | 2 | 70 | 8.90e-22 | 90.1 |
| MsG0680030559.01.T01 | MTR_8g041770 | 23.871 | 465 | 302 | 15 | 11 | 461 | 4 | 430 | 6.07e-21 | 95.5 |
| MsG0680030559.01.T01 | MTR_4g094842 | 24.558 | 452 | 289 | 14 | 22 | 450 | 15 | 437 | 1.24e-20 | 94.4 |
| MsG0680030559.01.T01 | MTR_3g101930 | 26.205 | 477 | 273 | 18 | 11 | 450 | 10 | 444 | 1.72e-20 | 94.0 |
| MsG0680030559.01.T01 | MTR_6g045553 | 26.269 | 453 | 269 | 18 | 34 | 447 | 35 | 461 | 3.64e-20 | 93.2 |
| MsG0680030559.01.T01 | MTR_4g094835 | 24.609 | 447 | 264 | 15 | 34 | 450 | 32 | 435 | 2.58e-18 | 87.4 |
| MsG0680030559.01.T01 | MTR_7g014220 | 69.811 | 53 | 16 | 0 | 28 | 80 | 12 | 64 | 1.10e-17 | 78.2 |
| MsG0680030559.01.T01 | MTR_8g096530 | 25.121 | 414 | 272 | 14 | 46 | 450 | 38 | 422 | 3.84e-17 | 84.0 |
| MsG0680030559.01.T01 | MTR_7g084940 | 26.799 | 403 | 246 | 16 | 70 | 454 | 69 | 440 | 4.52e-17 | 83.6 |
| MsG0680030559.01.T01 | MTR_4g022800 | 24.256 | 437 | 276 | 16 | 42 | 450 | 22 | 431 | 2.91e-16 | 81.3 |
| MsG0680030559.01.T01 | MTR_3g070170 | 23.598 | 428 | 264 | 17 | 58 | 447 | 54 | 456 | 2.05e-15 | 78.6 |
| MsG0680030559.01.T01 | MTR_3g435290 | 34.146 | 123 | 78 | 2 | 339 | 461 | 1 | 120 | 2.24e-15 | 72.8 |
| MsG0680030559.01.T01 | MTR_7g086000 | 24.769 | 432 | 248 | 16 | 73 | 461 | 63 | 460 | 4.38e-15 | 77.8 |
| MsG0680030559.01.T01 | MTR_3g026950 | 23.095 | 420 | 268 | 15 | 67 | 454 | 51 | 447 | 4.73e-15 | 77.4 |
| MsG0680030559.01.T01 | MTR_4g094828 | 23.457 | 405 | 261 | 14 | 30 | 413 | 25 | 401 | 5.41e-15 | 77.4 |
| MsG0680030559.01.T01 | MTR_5g026320 | 26.179 | 424 | 250 | 18 | 34 | 434 | 41 | 424 | 5.85e-15 | 77.0 |
| MsG0680030559.01.T01 | MTR_3g026310 | 23.110 | 463 | 287 | 17 | 23 | 450 | 9 | 437 | 6.08e-15 | 77.0 |
| MsG0680030559.01.T01 | MTR_0190s0020 | 24.318 | 403 | 253 | 12 | 67 | 449 | 60 | 430 | 1.21e-14 | 76.3 |
| MsG0680030559.01.T01 | MTR_7g085990 | 24.537 | 432 | 270 | 15 | 65 | 461 | 50 | 460 | 1.00e-13 | 73.6 |
| MsG0680030559.01.T01 | MTR_7g027175 | 23.919 | 347 | 235 | 9 | 70 | 410 | 68 | 391 | 1.21e-13 | 73.2 |
| MsG0680030559.01.T01 | MTR_4g127650 | 22.609 | 460 | 291 | 17 | 32 | 458 | 29 | 456 | 2.24e-13 | 72.4 |
| MsG0680030559.01.T01 | MTR_3g049150 | 23.239 | 426 | 265 | 13 | 66 | 458 | 51 | 447 | 4.88e-13 | 71.2 |
| MsG0680030559.01.T01 | MTR_2g089745 | 45.205 | 73 | 40 | 0 | 389 | 461 | 8 | 80 | 9.18e-13 | 64.3 |
| MsG0680030559.01.T01 | MTR_7g084910 | 23.770 | 366 | 230 | 12 | 55 | 411 | 55 | 380 | 2.87e-12 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030559.01.T01 | AT5G39090 | 38.767 | 454 | 233 | 13 | 26 | 459 | 20 | 448 | 4.47e-93 | 289 |
| MsG0680030559.01.T01 | AT3G29635 | 38.298 | 470 | 257 | 13 | 9 | 461 | 4 | 457 | 3.74e-91 | 285 |
| MsG0680030559.01.T01 | AT5G39080 | 36.325 | 468 | 264 | 15 | 7 | 454 | 4 | 457 | 1.34e-88 | 278 |
| MsG0680030559.01.T01 | AT3G29670 | 35.667 | 457 | 278 | 10 | 10 | 460 | 5 | 451 | 3.27e-84 | 266 |
| MsG0680030559.01.T01 | AT5G39050 | 38.075 | 478 | 263 | 16 | 3 | 460 | 5 | 469 | 7.53e-82 | 261 |
| MsG0680030559.01.T01 | AT3G29680 | 37.923 | 443 | 245 | 12 | 26 | 454 | 17 | 443 | 4.44e-81 | 258 |
| MsG0680030559.01.T01 | AT5G61160 | 33.043 | 460 | 285 | 10 | 9 | 458 | 4 | 450 | 1.04e-80 | 257 |
| MsG0680030559.01.T01 | AT3G29590 | 34.105 | 475 | 271 | 14 | 1 | 459 | 1 | 449 | 1.69e-76 | 246 |
| MsG0680030559.01.T01 | AT1G03940 | 32.708 | 480 | 287 | 14 | 1 | 459 | 1 | 465 | 1.77e-71 | 234 |
| MsG0680030559.01.T01 | AT1G03495 | 33.524 | 349 | 200 | 13 | 1 | 332 | 1 | 334 | 1.06e-44 | 160 |
| MsG0680030559.01.T01 | AT5G23940 | 25.216 | 464 | 275 | 19 | 21 | 448 | 12 | 439 | 2.87e-24 | 105 |
| MsG0680030559.01.T01 | AT3G29720 | 35.323 | 201 | 117 | 7 | 91 | 288 | 11 | 201 | 3.93e-22 | 94.7 |
| MsG0680030559.01.T01 | AT5G48930 | 25.647 | 425 | 278 | 17 | 38 | 450 | 28 | 426 | 3.26e-21 | 96.3 |
| MsG0680030559.01.T01 | AT5G07850 | 26.115 | 471 | 286 | 19 | 9 | 452 | 9 | 444 | 6.19e-19 | 89.4 |
| MsG0680030559.01.T01 | AT5G67150 | 25.694 | 432 | 285 | 12 | 34 | 452 | 29 | 437 | 2.17e-18 | 87.8 |
| MsG0680030559.01.T01 | AT5G42830 | 25.416 | 421 | 244 | 16 | 64 | 452 | 58 | 440 | 2.53e-18 | 87.4 |
| MsG0680030559.01.T01 | AT2G39980 | 23.218 | 435 | 279 | 13 | 59 | 452 | 51 | 471 | 1.18e-17 | 85.9 |
| MsG0680030559.01.T01 | AT2G19070 | 24.832 | 447 | 269 | 18 | 47 | 458 | 37 | 451 | 1.42e-17 | 85.1 |
| MsG0680030559.01.T01 | AT3G29690 | 30.323 | 155 | 102 | 2 | 307 | 460 | 31 | 180 | 4.34e-17 | 79.3 |
| MsG0680030559.01.T01 | AT3G29636 | 30.000 | 230 | 90 | 7 | 246 | 461 | 23 | 195 | 8.81e-17 | 79.0 |
| MsG0680030559.01.T01 | AT3G48720 | 23.797 | 395 | 264 | 12 | 70 | 454 | 61 | 428 | 3.00e-16 | 81.3 |
| MsG0680030559.01.T01 | AT5G41040 | 24.029 | 412 | 248 | 13 | 70 | 456 | 69 | 440 | 1.55e-15 | 79.0 |
| MsG0680030559.01.T01 | AT5G41040 | 24.029 | 412 | 248 | 13 | 70 | 456 | 85 | 456 | 2.32e-15 | 78.6 |
| MsG0680030559.01.T01 | AT5G57840 | 23.144 | 458 | 291 | 18 | 27 | 458 | 16 | 438 | 6.06e-15 | 77.0 |
| MsG0680030559.01.T01 | AT3G50270 | 26.329 | 414 | 250 | 16 | 21 | 410 | 15 | 397 | 1.09e-14 | 76.3 |
| MsG0680030559.01.T01 | AT1G31490 | 26.168 | 428 | 249 | 20 | 31 | 434 | 26 | 410 | 1.95e-14 | 75.5 |
| MsG0680030559.01.T01 | AT5G38130 | 23.697 | 422 | 275 | 15 | 37 | 442 | 52 | 442 | 7.43e-13 | 70.9 |
| MsG0680030559.01.T01 | AT3G50280 | 27.240 | 279 | 164 | 9 | 147 | 413 | 14 | 265 | 1.83e-12 | 68.6 |
| MsG0680030559.01.T01 | AT3G50280 | 27.240 | 279 | 164 | 9 | 147 | 413 | 144 | 395 | 2.72e-12 | 68.9 |
| MsG0680030559.01.T01 | AT5G67160 | 23.077 | 403 | 258 | 14 | 24 | 410 | 16 | 382 | 8.56e-12 | 67.4 |
| MsG0680030559.01.T01 | AT5G63560 | 22.933 | 375 | 244 | 13 | 47 | 410 | 40 | 380 | 2.09e-11 | 66.2 |
| MsG0680030559.01.T01 | AT1G03390 | 25.056 | 447 | 262 | 22 | 22 | 439 | 30 | 432 | 5.15e-11 | 65.1 |
Find 63 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTTCTCCTATGTATTT+TGG | 0.099812 | 6:-5288214 | MsG0680030559.01.T01:CDS |
| CCAAAGTTTGAAGCTTTAAA+TGG | 0.182323 | 6:+5287799 | None:intergenic |
| CTCTTGCTGGTAACATAATT+TGG | 0.192500 | 6:-5288930 | MsG0680030559.01.T01:CDS |
| TCACTTTCTTTGATCTTCTA+TGG | 0.215533 | 6:-5289077 | MsG0680030559.01.T01:CDS |
| GTTGATGCTTTGATGAATTT+TGG | 0.216352 | 6:-5288505 | MsG0680030559.01.T01:CDS |
| ATTGAGATTGGTTTGGCTTT+AGG | 0.240774 | 6:-5287872 | MsG0680030559.01.T01:CDS |
| TGGTTAGCTACTATGTTTAA+TGG | 0.256858 | 6:-5288052 | MsG0680030559.01.T01:CDS |
| ATTCAAGTCCTTGAACAAAA+AGG | 0.268365 | 6:+5287822 | None:intergenic |
| GAAGTCATATCAACTTTCTT+AGG | 0.271346 | 6:+5287950 | None:intergenic |
| TACTATGTTTAATGGTAAAA+AGG | 0.281901 | 6:-5288044 | MsG0680030559.01.T01:CDS |
| GTTAAAATTTCCTCTATTGC+TGG | 0.309053 | 6:-5288019 | MsG0680030559.01.T01:CDS |
| AAGATCTTTGAAACTTTCAT+TGG | 0.324573 | 6:+5288837 | None:intergenic |
| GAAAGTGAGTGTTTGAGATT+TGG | 0.329141 | 6:+5288976 | None:intergenic |
| ATTGCAAACTTCAACAACTT+TGG | 0.345314 | 6:+5289155 | None:intergenic |
| GAGGTTTATAGTATTGATTT+TGG | 0.348010 | 6:-5287983 | MsG0680030559.01.T01:CDS |
| TAATAAAAGTCTCAAGGTTT+GGG | 0.355038 | 6:-5288473 | MsG0680030559.01.T01:CDS |
| TCACACCATGCTGGTTTAGA+TGG | 0.356316 | 6:-5288682 | MsG0680030559.01.T01:CDS |
| CAACACTTTCTCCCTCTTGC+TGG | 0.361140 | 6:-5288943 | MsG0680030559.01.T01:CDS |
| AAGAAATTAGTTGGAAAAGA+TGG | 0.370504 | 6:-5288154 | MsG0680030559.01.T01:CDS |
| TACTATAAACCTCAAACCTT+GGG | 0.372685 | 6:+5287993 | None:intergenic |
| AACAATGGTGGAATTGAGAT+TGG | 0.373662 | 6:-5287884 | MsG0680030559.01.T01:CDS |
| ACCACTCATAAACTATGTTA+AGG | 0.373728 | 6:-5288890 | MsG0680030559.01.T01:CDS |
| ATAATAAAAGTCTCAAGGTT+TGG | 0.375427 | 6:-5288474 | MsG0680030559.01.T01:CDS |
| AGAATTAGAACATGCAATAT+AGG | 0.378155 | 6:+5288627 | None:intergenic |
| TGCTGGATCCCCAAGGTTTG+AGG | 0.379703 | 6:-5288002 | MsG0680030559.01.T01:CDS |
| CTTGATACAAAGAAATTAGT+TGG | 0.386537 | 6:-5288163 | MsG0680030559.01.T01:CDS |
| GGATGAATGAAGCTTTGAAT+AGG | 0.393461 | 6:-5288111 | MsG0680030559.01.T01:CDS |
| GATGAATGAAGCTTTGAATA+GGG | 0.427868 | 6:-5288110 | MsG0680030559.01.T01:CDS |
| GGCTTTAGGTAAGGAAGAGA+TGG | 0.436688 | 6:-5287858 | MsG0680030559.01.T01:CDS |
| CCTTGGGGATCCAGCAATAG+AGG | 0.437743 | 6:+5288009 | None:intergenic |
| ATACTATAAACCTCAAACCT+TGG | 0.440758 | 6:+5287992 | None:intergenic |
| AAAGTGAGAGGAAGTGAGGT+TGG | 0.449935 | 6:+5289093 | None:intergenic |
| ATGACTTCAACTGATAAAAC+AGG | 0.450314 | 6:-5287935 | MsG0680030559.01.T01:CDS |
| TGTTGAATGGAGCTAAAGAT+TGG | 0.451673 | 6:-5288072 | MsG0680030559.01.T01:CDS |
| CCACTCATAAACTATGTTAA+GGG | 0.455542 | 6:-5288889 | MsG0680030559.01.T01:CDS |
| ATTGGACTCAACAACATTGA+AGG | 0.466727 | 6:+5288855 | None:intergenic |
| TAGAAGAAAAGACGTTCAAC+TGG | 0.473498 | 6:+5289042 | None:intergenic |
| AAATTGTGTTACAGGACAAA+AGG | 0.481421 | 6:-5288191 | MsG0680030559.01.T01:CDS |
| TTGAATAGGGTGAAGAATGA+TGG | 0.499745 | 6:-5288097 | MsG0680030559.01.T01:CDS |
| ATCACTTTGTTTCCAAACAA+TGG | 0.504774 | 6:-5288724 | MsG0680030559.01.T01:CDS |
| GATTGGTTTGGCTTTAGGTA+AGG | 0.510243 | 6:-5287867 | MsG0680030559.01.T01:CDS |
| AAGAATGATGGAGTGTTGAA+TGG | 0.511427 | 6:-5288085 | MsG0680030559.01.T01:CDS |
| TGGTGGAATTGAGATTGGTT+TGG | 0.519435 | 6:-5287879 | MsG0680030559.01.T01:CDS |
| AAAGAAAGTGAGAGGAAGTG+AGG | 0.528013 | 6:+5289089 | None:intergenic |
| GGACCATGATTTCATAAAAG+TGG | 0.528105 | 6:+5288648 | None:intergenic |
| CCTCTATTGCTGGATCCCCA+AGG | 0.546894 | 6:-5288009 | MsG0680030559.01.T01:CDS |
| GGAATATCATCACACCATGC+TGG | 0.550715 | 6:-5288691 | MsG0680030559.01.T01:CDS |
| TGATTCTTCATGTTGTGCCA+TGG | 0.552657 | 6:+5289179 | None:intergenic |
| AAGAAAAGACGTTCAACTGG+TGG | 0.567805 | 6:+5289045 | None:intergenic |
| AGCAAAAGCTGTGCAACCAA+AGG | 0.568923 | 6:-5288290 | MsG0680030559.01.T01:CDS |
| CCCTTAACATAGTTTATGAG+TGG | 0.569875 | 6:+5288889 | None:intergenic |
| ACCAAATAATAAAAGTCTCA+AGG | 0.572931 | 6:-5288479 | MsG0680030559.01.T01:CDS |
| GAAAGTAGGAATAACAATGG+TGG | 0.573498 | 6:-5287896 | MsG0680030559.01.T01:CDS |
| ACACAATTTCCAAAATACAT+AGG | 0.574370 | 6:+5288205 | None:intergenic |
| AGAAGATCAAAGAAAGTGAG+AGG | 0.592860 | 6:+5289081 | None:intergenic |
| CAAATTATGTTACCAGCAAG+AGG | 0.594605 | 6:+5288931 | None:intergenic |
| AAATACATAGGAGAAATTGA+AGG | 0.601552 | 6:+5288217 | None:intergenic |
| CCATGATTTCATAAAAGTGG+TGG | 0.604119 | 6:+5288651 | None:intergenic |
| AGTGAAAGTAGGAATAACAA+TGG | 0.647980 | 6:-5287899 | MsG0680030559.01.T01:CDS |
| ACTATAAACCTCAAACCTTG+GGG | 0.653071 | 6:+5287994 | None:intergenic |
| AAATTATGTTACCAGCAAGA+GGG | 0.657111 | 6:+5288932 | None:intergenic |
| TTGATAGAAGATCATCATGG+TGG | 0.686075 | 6:-5288544 | MsG0680030559.01.T01:CDS |
| TCATTGATAGAAGATCATCA+TGG | 0.705951 | 6:-5288547 | MsG0680030559.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATAAAAAAGATGAATCA+AGG | + | Chr6:5288392-5288411 | None:intergenic | 20.0% |
| !! | ATCAATGATCTATCAAAAAA+AGG | + | Chr6:5288432-5288451 | None:intergenic | 20.0% |
| !! | TACTATGTTTAATGGTAAAA+AGG | - | Chr6:5288947-5288966 | MsG0680030559.01.T01:CDS | 20.0% |
| !!! | TATAGTATTGATTTTGGATT+TGG | - | Chr6:5289014-5289033 | MsG0680030559.01.T01:CDS | 20.0% |
| !!! | TTAAGCTTTTGAATATTTGA+AGG | + | Chr6:5288597-5288616 | None:intergenic | 20.0% |
| ! | AAATACATAGGAGAAATTGA+AGG | + | Chr6:5288777-5288796 | None:intergenic | 25.0% |
| ! | AAGAAATTAGTTGGAAAAGA+TGG | - | Chr6:5288837-5288856 | MsG0680030559.01.T01:CDS | 25.0% |
| ! | AAGAGTTTCATAGAAAAAAG+AGG | + | Chr6:5287992-5288011 | None:intergenic | 25.0% |
| ! | ACACAATTTCCAAAATACAT+AGG | + | Chr6:5288789-5288808 | None:intergenic | 25.0% |
| ! | ACCAAATAATAAAAGTCTCA+AGG | - | Chr6:5288512-5288531 | MsG0680030559.01.T01:CDS | 25.0% |
| ! | AGAATTAGAACATGCAATAT+AGG | + | Chr6:5288367-5288386 | None:intergenic | 25.0% |
| ! | AGAGTTTCATAGAAAAAAGA+GGG | + | Chr6:5287991-5288010 | None:intergenic | 25.0% |
| ! | ATAATAAAAGTCTCAAGGTT+TGG | - | Chr6:5288517-5288536 | MsG0680030559.01.T01:CDS | 25.0% |
| ! | CTTGATACAAAGAAATTAGT+TGG | - | Chr6:5288828-5288847 | MsG0680030559.01.T01:CDS | 25.0% |
| ! | TAATAAAAGTCTCAAGGTTT+GGG | - | Chr6:5288518-5288537 | MsG0680030559.01.T01:CDS | 25.0% |
| !! | ACCTTGAGACTTTTATTATT+TGG | + | Chr6:5288516-5288535 | None:intergenic | 25.0% |
| !! | AGTGTTTTTCATATTTAGTG+TGG | - | Chr6:5288731-5288750 | MsG0680030559.01.T01:CDS | 25.0% |
| !! | TCAATTTCTCCTATGTATTT+TGG | - | Chr6:5288777-5288796 | MsG0680030559.01.T01:CDS | 25.0% |
| !!! | AAGATCTTTGAAACTTTCAT+TGG | + | Chr6:5288157-5288176 | None:intergenic | 25.0% |
| !!! | ATCTATCAAAAAAAGGTGTT+AGG | + | Chr6:5288425-5288444 | None:intergenic | 25.0% |
| !!! | GAGGTTTATAGTATTGATTT+TGG | - | Chr6:5289008-5289027 | MsG0680030559.01.T01:CDS | 25.0% |
| !!! | GATTTTTAAGTGCTTTAGAA+GGG | - | Chr6:5288859-5288878 | MsG0680030559.01.T01:CDS | 25.0% |
| !!! | TATTTTGGAAATTGTGTTAC+AGG | - | Chr6:5288792-5288811 | MsG0680030559.01.T01:CDS | 25.0% |
| AAAAACACTTCTTCAACCTT+TGG | + | Chr6:5288720-5288739 | None:intergenic | 30.0% | |
| AAATTATGTTACCAGCAAGA+GGG | + | Chr6:5288062-5288081 | None:intergenic | 30.0% | |
| AAATTGTGTTACAGGACAAA+AGG | - | Chr6:5288800-5288819 | MsG0680030559.01.T01:CDS | 30.0% | |
| ACCACTCATAAACTATGTTA+AGG | - | Chr6:5288101-5288120 | MsG0680030559.01.T01:CDS | 30.0% | |
| AGTGAAAGTAGGAATAACAA+TGG | - | Chr6:5289092-5289111 | MsG0680030559.01.T01:CDS | 30.0% | |
| ATACTATAAACCTCAAACCT+TGG | + | Chr6:5289002-5289021 | None:intergenic | 30.0% | |
| ATGACTTCAACTGATAAAAC+AGG | - | Chr6:5289056-5289075 | MsG0680030559.01.T01:CDS | 30.0% | |
| ATTCAAGTCCTTGAACAAAA+AGG | + | Chr6:5289172-5289191 | None:intergenic | 30.0% | |
| ATTGCAAACTTCAACAACTT+TGG | + | Chr6:5287839-5287858 | None:intergenic | 30.0% | |
| CCACTCATAAACTATGTTAA+GGG | - | Chr6:5288102-5288121 | MsG0680030559.01.T01:CDS | 30.0% | |
| GAAGTCATATCAACTTTCTT+AGG | + | Chr6:5289044-5289063 | None:intergenic | 30.0% | |
| GTTAAAATTTCCTCTATTGC+TGG | - | Chr6:5288972-5288991 | MsG0680030559.01.T01:CDS | 30.0% | |
| TACTATAAACCTCAAACCTT+GGG | + | Chr6:5289001-5289020 | None:intergenic | 30.0% | |
| TCACTTTCTTTGATCTTCTA+TGG | - | Chr6:5287914-5287933 | MsG0680030559.01.T01:CDS | 30.0% | |
| TCATTGATAGAAGATCATCA+TGG | - | Chr6:5288444-5288463 | MsG0680030559.01.T01:CDS | 30.0% | |
| TTCAAGTCCTTGAACAAAAA+GGG | + | Chr6:5289171-5289190 | None:intergenic | 30.0% | |
| ! | ATCACTTTGTTTCCAAACAA+TGG | - | Chr6:5288267-5288286 | MsG0680030559.01.T01:CDS | 30.0% |
| ! | CATTTTCTCTAAGTGAAAGT+AGG | - | Chr6:5289081-5289100 | MsG0680030559.01.T01:CDS | 30.0% |
| ! | GTTGATGCTTTGATGAATTT+TGG | - | Chr6:5288486-5288505 | MsG0680030559.01.T01:CDS | 30.0% |
| ! | TCATGTGAAGTTTTCAAAAG+CGG | + | Chr6:5288222-5288241 | None:intergenic | 30.0% |
| ! | TGGTTAGCTACTATGTTTAA+TGG | - | Chr6:5288939-5288958 | MsG0680030559.01.T01:CDS | 30.0% |
| ! | TTTTCTACCCTTTTTGTTCA+AGG | - | Chr6:5289161-5289180 | MsG0680030559.01.T01:CDS | 30.0% |
| !! | GATGAATGAAGCTTTGAATA+GGG | - | Chr6:5288881-5288900 | MsG0680030559.01.T01:CDS | 30.0% |
| !! | TTTAATTGCATCGTTTTTCG+TGG | + | Chr6:5288556-5288575 | None:intergenic | 30.0% |
| !!! | AAAAAAGGTGTTAGGTTTTC+AGG | + | Chr6:5288417-5288436 | None:intergenic | 30.0% |
| !!! | GGATTTTTAAGTGCTTTAGA+AGG | - | Chr6:5288858-5288877 | MsG0680030559.01.T01:CDS | 30.0% |
| !!! | GTTTTTGTTGGTTCATGAAA+TGG | + | Chr6:5287868-5287887 | None:intergenic | 30.0% |
| ACTATAAACCTCAAACCTTG+GGG | + | Chr6:5289000-5289019 | None:intergenic | 35.0% | |
| AGAAGATCAAAGAAAGTGAG+AGG | + | Chr6:5287913-5287932 | None:intergenic | 35.0% | |
| ATAGAAAAAAGAGGGTGAGT+TGG | + | Chr6:5287983-5288002 | None:intergenic | 35.0% | |
| ATTGGACTCAACAACATTGA+AGG | + | Chr6:5288139-5288158 | None:intergenic | 35.0% | |
| CAAATTATGTTACCAGCAAG+AGG | + | Chr6:5288063-5288082 | None:intergenic | 35.0% | |
| CCAATGCAAAAACCATTGTT+TGG | + | Chr6:5288282-5288301 | None:intergenic | 35.0% | |
| CCATGATTTCATAAAAGTGG+TGG | + | Chr6:5288343-5288362 | None:intergenic | 35.0% | |
| CCCTTAACATAGTTTATGAG+TGG | + | Chr6:5288105-5288124 | None:intergenic | 35.0% | |
| CTCTTGCTGGTAACATAATT+TGG | - | Chr6:5288061-5288080 | MsG0680030559.01.T01:CDS | 35.0% | |
| GAAAGTAGGAATAACAATGG+TGG | - | Chr6:5289095-5289114 | MsG0680030559.01.T01:CDS | 35.0% | |
| GGACCATGATTTCATAAAAG+TGG | + | Chr6:5288346-5288365 | None:intergenic | 35.0% | |
| TAGAAGAAAAGACGTTCAAC+TGG | + | Chr6:5287952-5287971 | None:intergenic | 35.0% | |
| TGTTGAATGGAGCTAAAGAT+TGG | - | Chr6:5288919-5288938 | MsG0680030559.01.T01:CDS | 35.0% | |
| TTGAATAGGGTGAAGAATGA+TGG | - | Chr6:5288894-5288913 | MsG0680030559.01.T01:CDS | 35.0% | |
| TTGATAGAAGATCATCATGG+TGG | - | Chr6:5288447-5288466 | MsG0680030559.01.T01:CDS | 35.0% | |
| ! | AACAATGGTGGAATTGAGAT+TGG | - | Chr6:5289107-5289126 | MsG0680030559.01.T01:CDS | 35.0% |
| ! | ATTTTCCATCTAAACCAGCA+TGG | + | Chr6:5288317-5288336 | None:intergenic | 35.0% |
| ! | CCAAACAATGGTTTTTGCAT+TGG | - | Chr6:5288279-5288298 | MsG0680030559.01.T01:CDS | 35.0% |
| ! | GAAAGTGAGTGTTTGAGATT+TGG | + | Chr6:5288018-5288037 | None:intergenic | 35.0% |
| ! | GATGCTTTGATGAATTTTGG+TGG | - | Chr6:5288489-5288508 | MsG0680030559.01.T01:CDS | 35.0% |
| ! | GGTGATTGAGAAGTTTTTGT+TGG | + | Chr6:5287880-5287899 | None:intergenic | 35.0% |
| !! | AAGAATGATGGAGTGTTGAA+TGG | - | Chr6:5288906-5288925 | MsG0680030559.01.T01:CDS | 35.0% |
| !! | ATTGAGATTGGTTTGGCTTT+AGG | - | Chr6:5289119-5289138 | MsG0680030559.01.T01:CDS | 35.0% |
| !! | CCACCACTTTTATGAAATCA+TGG | - | Chr6:5288340-5288359 | MsG0680030559.01.T01:CDS | 35.0% |
| !! | GGATGAATGAAGCTTTGAAT+AGG | - | Chr6:5288880-5288899 | MsG0680030559.01.T01:CDS | 35.0% |
| !!! | AGTGGTTTTGATGAATCACT+AGG | + | Chr6:5288087-5288106 | None:intergenic | 35.0% |
| !!! | CTCAAGGTTTGGGATTTTTT+CGG | - | Chr6:5288528-5288547 | MsG0680030559.01.T01:CDS | 35.0% |
| AAAGAAAGTGAGAGGAAGTG+AGG | + | Chr6:5287905-5287924 | None:intergenic | 40.0% | |
| AAGAAAAGACGTTCAACTGG+TGG | + | Chr6:5287949-5287968 | None:intergenic | 40.0% | |
| ! | TGGTGGAATTGAGATTGGTT+TGG | - | Chr6:5289112-5289131 | MsG0680030559.01.T01:CDS | 40.0% |
| !! | GATTGGTTTGGCTTTAGGTA+AGG | - | Chr6:5289124-5289143 | MsG0680030559.01.T01:CDS | 40.0% |
| AAAGTGAGAGGAAGTGAGGT+TGG | + | Chr6:5287901-5287920 | None:intergenic | 45.0% | |
| AGCAAAAGCTGTGCAACCAA+AGG | - | Chr6:5288701-5288720 | MsG0680030559.01.T01:CDS | 45.0% | |
| GGAATATCATCACACCATGC+TGG | - | Chr6:5288300-5288319 | MsG0680030559.01.T01:CDS | 45.0% | |
| !! | GGCTTTAGGTAAGGAAGAGA+TGG | - | Chr6:5289133-5289152 | MsG0680030559.01.T01:CDS | 45.0% |
| !! | TCACACCATGCTGGTTTAGA+TGG | - | Chr6:5288309-5288328 | MsG0680030559.01.T01:CDS | 45.0% |
| CAACACTTTCTCCCTCTTGC+TGG | - | Chr6:5288048-5288067 | MsG0680030559.01.T01:CDS | 50.0% | |
| CCTCTATTGCTGGATCCCCA+AGG | - | Chr6:5288982-5289001 | MsG0680030559.01.T01:CDS | 55.0% | |
| CCTTGGGGATCCAGCAATAG+AGG | + | Chr6:5288985-5289004 | None:intergenic | 55.0% | |
| TGCTGGATCCCCAAGGTTTG+AGG | - | Chr6:5288989-5289008 | MsG0680030559.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 5287814 | 5289199 | 5287814 | ID=MsG0680030559.01;Name=MsG0680030559.01 |
| Chr6 | mRNA | 5287814 | 5289199 | 5287814 | ID=MsG0680030559.01.T01;Parent=MsG0680030559.01;Name=MsG0680030559.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|461 |
| Chr6 | exon | 5287814 | 5289199 | 5287814 | ID=MsG0680030559.01.T01:exon:4363;Parent=MsG0680030559.01.T01 |
| Chr6 | CDS | 5287814 | 5289199 | 5287814 | ID=MsG0680030559.01.T01:cds;Parent=MsG0680030559.01.T01 |
| Gene Sequence |
| Protein sequence |
